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Foliar Fertilization of Dual-Labeled Organic and Inorganic N in Rice; Mechanisms of Transport and Assimilation 有机氮和无机氮双重标记在水稻叶面施肥中的应用;迁移和同化机制
Q2 Agricultural and Biological Sciences Pub Date : 2022-04-01 DOI: 10.17957/ijab/15.1923
Zhaohui Zou
The absorption, transport, and assimilation mechanism of organic and inorganic nitrogen(N) in rice organs were analyzed by applying 13C-15N dual-labeled organic and inorganic N directly to the leaves of rice plants (variety: C Liangyou 266) at tillering stage based on isotope tracing. The findings suggest that the dry weight and N accumulation of various rice organs under glycine N and ammonium N treatments were significantly higher than other treatments; the dry weight and N accumulation in rice organs followed the pattern of “leaf > root > stem”, and there were no significant differences between control and nitrate N treatment. The 15N increments were detected in the roots, stems and leaves of all treatments, showing a certain pattern of “leaf > stem > root”; there were significant differences between the 15N increments of various organs (P < 0.01). The 13C increment/15N increment ratios of rice root, stem, leaf, and whole plant were 0.108, 0.158, 0.178, and 0.161 respectively. For rice plants treated by glycine N and ammonium N, the activity of GOT, GPT and GDH peaked in leaves, followed by stems and then roots; while the pattern of activity for GOT, GPT and GDH in the control (Ck) and nitrate N group was in following order “leaf > root > stem”. The results showed that rice leaves directly absorb and utilize molecular glycine and the absorption rate of glycine is significantly higher than ammonium N or nitrate N. Molecular organic N absorbed into rice leaves would be transported to roots; the transportability of N in rice plants ranked in descending order is as follows: amino acid N > ammonium N > nitrate N. © 2022 Friends Science Publishers
采用13C-15N双标记有机和无机氮在水稻分蘖期直接施用于品种C两优266叶片上的同位素示踪方法,分析了有机和无机氮在水稻各器官中的吸收、转运和同化机制。结果表明:甘氨酸氮和铵态氮处理水稻各器官干重和氮积累量显著高于其他处理;水稻各器官的干重和氮积累均呈现“叶>根>茎”的格局,对照与硝态氮处理间差异不显著。各处理的根、茎、叶均有15N增量,呈现出一定的“叶>茎>根”的格局;各脏器15N增量间差异极显著(P < 0.01)。水稻根、茎、叶和全株的13C增量/15N增量比值分别为0.108、0.158、0.178和0.161。在甘氨酸N和铵态氮处理下,水稻叶片中GOT、GPT和GDH活性最高,茎部次之,根部次之;对照(Ck)和硝态氮组的GOT、GPT和GDH活性为“叶>根>茎”。结果表明:水稻叶片直接吸收和利用分子甘氨酸,且甘氨酸的吸收率显著高于铵态氮和硝态氮;水稻植株中N的可转运性由高到低依次为:氨基酸N >铵态N >硝态N©2022 Friends Science Publishers
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引用次数: 0
Potential of Bipolaris bicolor in Combination with Ametryn for Inhibition of Glyphosate-Resistant Goosegrass (Eleusine indica) Biotypes 双色双孢菌与阿米特灵联合抑制草甘膦抗性鹅膏草(Eleusine indica)生物型的潜力
Q2 Agricultural and Biological Sciences Pub Date : 2022-03-01 DOI: 10.17957/ijab/15.1908
M. Fakri
Fungal pathogens have been investigated previously for biological control of goosegrass (Eleusine indica), but there is little research on the compatibility of herbicides with fungal pathogen Bipolaris bicolor for control of glyphosate-resistant E. indica. The aims of this study were to 1) examine the in vitro compatibility of Bipolaris bicolor and three herbicides (ametryn, flumiozaxin and sodium chlorate) and 2) determine the efficacy of a combination of B. bicolor and ametryn for inhibiting glyphosate-resistant E. indica biotypes in the greenhouse. Mycelial growth inhibition area was measured after B. bicolor spores were treated with ametryn, flumiozaxin, or sodium chlorate at one-fifth of their recommended rates. The findings showed that ametryn had the lowest inhibitory effect (10%), while flumioxazin and sodium chlorate had 80 and 30% inhibition, respectively, indicating that ametryn is the most compatible herbicide with B. bicolor. E. indica seedlings were then subjected to four treatments, including non-treated plants, at the 3 to 4-leaf stage where T1: negative control plant, T2: one-fifteenth of the recommended dose of ametryn, T3: B. bicolor spore suspension and T4: ametryn plus B. bicolor spore suspension. B. bicolor in combination with ametryn was the most effective treatment for E. indica inhibition one week after treatment. The treatment reduced seedling height, leaf greenness and shoot dry weight by 50 to 94% and caused plant death seven days after treatment. These findings suggest that B. bicolor in combination with ametryn is a viable integrated strategy to control the glyphosate-resistant E. indica biotype. © 2022 Friends Science Publishers
以前已经研究过真菌病原体对鹅草(Eleusine indica)的生物防治,但很少研究除草剂与真菌病原体双色双孢菌(Bipolaris bicolor)对草甘膦抗性E.indica的兼容性。本研究的目的是:1)检验双色双孢菌与三种除草剂(屈曲明、氟米扎新和氯酸钠)的体外兼容性;2)确定双色双胞菌和屈曲明组合在温室中抑制草甘膦抗性E.indica生物型的效力。用屈光不正、氟米奥扎新或氯酸钠以其推荐速率的五分之一处理双色芽孢杆菌孢子后,测量菌丝生长抑制面积。研究结果表明,屈霉素的抑制作用最低(10%),氟米恶嗪和氯酸钠的抑制作用分别为80%和30%,表明屈霉素是与双色B.bicolor最相容的除草剂。然后,在3-4叶期对E.indica幼苗进行四种处理,包括未处理的植物,其中T1:阴性对照植物,T2:推荐剂量的十五分之一屈光不正,T3:双色芽孢杆菌悬浮液和T4:屈光不正氏杆菌加双色芽孢菌悬浮液。双色B.bicolor联合屈光不正是治疗后一周对印度洋地黄抑制作用最有效的治疗方法。该处理使幼苗高度、叶绿度和茎干重降低了50%至94%,并在处理后7天导致植株死亡。这些发现表明,双色B.bicolor与屈光不正联合使用是控制草甘膦抗性E.indica生物型的可行综合策略。©2022 Friends Science出版社
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引用次数: 0
Review on Genomics, Production Potential and Usefulness of Camel as the Animal of Future 骆驼作为未来动物的基因组学、生产潜力和用途综述
Q2 Agricultural and Biological Sciences Pub Date : 2022-03-01 DOI: 10.17957/ijab/15.1916
T. Hussain
Food security issues are persistently emerging in proportionate to the growth of human population. This scenario demands a search for alternate and development of new food sources. Camel can therefore be the best alternative and beneficial addition to food supply chain providing milk and meat for humans. It is an imperative component of desert ecosystem with much better feed conversion ratio. Hence in arid zones, it provides more milk and meat with less consumption of feed and fodder. Moreover, Heat Shock proteins present in camel serve as molecular chaperones and strengthen its resistance capability against hostile desert environment ultimately facilitating its survival. Distinctive nutritional value of its milk is thought to have therapeutic attributes. Furthermore, camel milk inherently possesses antimicrobial agents which promote its antiviral and antibacterial capacity. Its unique adaptability and superiority over other livestock has compelled scientists for the last few decades to explore its hidden potential. Its proper breeding and farming infrastructure, well backed by scientific advancements, does not exist which need to be essentially developed and established, the initiative not possible without collaborative research efforts. Under present conditions and keeping in view the requirements of the masses, the investigative work should focus on its therapeutic, biological, and functional properties instead of pursuing trivial aspects. Successful management and efficient handling of these researchable avenues can facility efficient utilization of this animal to meet the ever-increasing food demands of the masses. This review article will highlight its wonderful adaptive features, genetic make-up, usefulness at present, and its potential for future food security. © 2022 Friends Science Publishers
与人口增长成比例的粮食安全问题不断出现。这种情况要求寻找替代和开发新的食物来源。因此,骆驼可以成为为人类提供牛奶和肉类的食品供应链的最佳替代品和有益的补充。它是荒漠生态系统的重要组成部分,具有较好的饲料转化率。因此,在干旱地区,它提供了更多的牛奶和肉类,消耗更少的饲料和饲料。此外,骆驼体内存在的热休克蛋白作为分子伴侣,增强了骆驼对恶劣沙漠环境的抵抗能力,最终促进了骆驼的生存。牛奶独特的营养价值被认为具有治疗作用。此外,骆驼奶本身具有促进其抗病毒和抗菌能力的抗菌剂。它独特的适应性和优于其他牲畜的优势迫使科学家们在过去的几十年里探索它隐藏的潜力。它不存在适当的育种和农业基础设施,这些基础设施得到了科学进步的充分支持,这些基础设施基本上需要发展和建立,没有合作研究努力,该倡议是不可能的。在目前条件下,从群众需求出发,研究工作应着眼于其治疗、生物学和功能特性,而不是追求鸡毛蒜皮的鸡毛蒜皮。成功地管理和有效地处理这些可研究的途径,可以促进这种动物的有效利用,以满足群众日益增长的食物需求。这篇综述文章将重点介绍其奇妙的适应特性、基因组成、目前的用途及其对未来粮食安全的潜力。©2022朋友科学出版社
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引用次数: 0
Estimating the Breeding Potency of a Soybean Core Set 大豆核心组合育种效能的估计
Q2 Agricultural and Biological Sciences Pub Date : 2022-03-01 DOI: 10.17957/ijab/15.1915
E. Vijayakumar
Multi-environment evaluation of a core set helps in the identification of trait-specific genetic stocks for further exploitation to sustain soybean productivity. TNAU soybean core set comprising of 50 soybean germplasm (thirty national and twenty international lines) was evaluated for variability pattern, trait association and adaptability using ten quantitative and twenty-one qualitative characters over environments, years and seasons to identify potential parents for utilization. Both quantitative and qualitative traits-based diversity analyses indicated the presence of adequate variability. The principal component analysis based on quantitative traits reduced the total variation into three major components (73.72%). Genotypes JS9305, JS(SH)99-02, IC16009 and TNAU20048 were tagged as highly divergent lines. Single plant yield (0.92) and number of pods per plant (0.92) contributed the maximum for the rotated PC1. The association analysis revealed that number of pods per plant (r = 0.88) and number of clusters per plant (r = 0.81) contributed significantly to the single plant yield. The qualitative cluster analysis divided the core set into eight clusters. A good variability was observed for plant type, pod color, seed coat color, and hilum color. In the validation experiments, a higher yield was witnessed in the hybridization involving distantly related accessions (14.1%) than the closely related parents (7.5%). It is concluded that the TNAU soybean core set has real breeding potency for future exploitation. The parents JS9305, JS(SH)99-02, IC16009 and TNAU20048 possessed a higher degree of divergence, heterosis with better adaptability and offers scope of breeding utility. © 2022 Friends Science Publishers
核心集的多环境评估有助于识别性状特异性遗传资源,以进一步开发,维持大豆生产力。利用环境、年份和季节的10个定量和21个定性特征,对由50个大豆种质(30个国家和20个国际品系)组成的TNAU大豆核心集的变异模式、性状关联和适应性进行了评估,以确定潜在的利用亲本。基于数量和质量性状的多样性分析都表明存在足够的变异性。基于数量性状的主成分分析将总变异减少为三个主要成分(73.72%)。基因型JS9305、JS(SH)99-02、IC16009和TNAU20048被标记为高度分化系。单株产量(0.92)和单株荚数(0.92。关联分析表明,单株荚数(r=0.88)和单株簇数(r=0.85)对单株产量有显著贡献。定性聚类分析将核心集合划分为八个聚类。观察到植物类型、荚色、种皮颜色和脐部颜色具有良好的变异性。在验证实验中,远缘材料的杂交产量(14.1%)高于近缘亲本(7.5%)。亲本JS9305、JS(SH)99-02、IC16009和TNAU20048具有较高的分化程度,杂种优势具有较好的适应性,具有一定的育种应用范围。©2022 Friends Science出版社
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引用次数: 0
High-Frequency Direct Organogenesis from Cotyledonary Node Explants and Plantlet Regeneration of Peanut (Arachis hypogaea) Cultivars 花生子叶节外植体高频直接器官发生及植株再生
Q2 Agricultural and Biological Sciences Pub Date : 2022-03-01 DOI: 10.17957/ijab/15.1906
Abraham Lamboro
The efficient plantlets regeneration of peanut is the most important and a primary step to successfully transform gene and apply recently developed genome editing techniques for crop yield improvement. The purpose of this research is to develop protocol for peanut cultivars applying different concentration of hormones for selected peanut cultivars and develop plantlets regeneration protocol. There is no previously reported protocol for the Chinese peanut cultivar N3 and Yu-hua-14. We optimized shoot and root regeneration protocol for two peanut cultivars. Both cultivars showed positive response for the cytokinin plant growth hormone 6-benzylaminopurine (BAP) and thidiazuron (TDZ). The highest shooting rate (97%) was found in a medium supplemented with 4 mg/L BAP and (94.33%) for 1 mg/L TDZ. Hence, more shoot initiation was observed at higher concentration of BAP as compared to TDZ. However, the maximum root regeneration (81%) was found on medium containing 0.3 mg/L 2, 4-dichlorophenoxyacetic acid (2,4-D) and the highest rooting rate (96.33%) was found in a medium containing 1 mg/L α-naphthalene acetic acid (NAA), indicating lower concentration of NAA induce more rooting rate than 2,4-D treatment. In this study, cotyledonary node was used, and this method was found to be efficient and rapid for in vitro peanut regeneration. © 2022 Friends Science Publishers
花生植株的高效再生是成功转化基因和应用最新开发的基因组编辑技术提高作物产量的最重要和第一步。本研究的目的是为选定的花生品种制定不同浓度的激素处理方案,并制定植株再生方案。中国花生品种N3和育花14的配种方案以前没有报道。对2个花生品种的茎、根再生方案进行了优化。两个品种对细胞分裂素、植物生长激素6-苄基氨基嘌呤(BAP)和硫代氮脲(TDZ)均呈阳性反应。在添加4 mg/L BAP和1 mg/L TDZ的培养基中,成活率最高,分别为97%和94.33%。因此,与TDZ相比,BAP浓度越高,嫩枝萌发率越高。而在含有0.3 mg/L 2,4-二氯苯氧乙酸(2,4- d)的培养基上生根率最高(81%),在含有1 mg/L α-萘乙酸(NAA)的培养基上生根率最高(96.33%),说明较低浓度NAA处理的生根率高于2,4- d处理。本研究采用子叶结法对花生进行体外再生,结果表明该方法高效、快速。©2022朋友科学出版社
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引用次数: 0
Agrobacterium-Mediated Genetic Transformation System of Amorpha fruticosa using Callus from the Cotyledonary Node 农杆菌介导的紫穗槐子叶结愈伤组织遗传转化体系研究
Q2 Agricultural and Biological Sciences Pub Date : 2022-03-01 DOI: 10.17957/ijab/15.1917
Yiteng Zhang
We used Agrobacterium-mediated infection of callus induced from the cotyledonary nodes of Amorpha fruticosa L. to study the β-glucuronidase gene (GUS)-integrated genetic transformation system. Transformed calluses were selected under 40 mg·L−1 kanamycin, differentiated into resistant adventitious buds, and developed into transformants. A single copy of gus was integrated in the genome of most T0 generation plants. Gus chemical staining analysis showed blue color in resistant calluses, adventitious buds, and the roots and leaves of transformed plants. This indicated gus overexpression driven by the 35S promotor and resultant β-glucuronidase activity. The genetic transformation system in this study could be used to study other functional genes of A. fruticosa and facilitate transgenic breeding for strain improvement. © 2022 Friends Science Publishers
利用农杆菌介导的紫穗槐子叶节愈伤组织侵染,研究了β-葡萄糖醛酸酶基因(GUS)整合的遗传转化体系。在40 mg·L−1卡那霉素作用下,转化愈伤组织分化为抗性不定芽,并发育为转化体。大多数第0代植物的基因组中都有gus的一个拷贝。Gus化学染色分析表明,转化植株的抗性愈伤组织、不定芽、根和叶呈蓝色。这表明gus过表达是由35S启动子驱动的,并由此产生β-葡萄糖醛酸酶活性。本研究建立的遗传转化体系可用于研究金银花的其他功能基因,为品种改良的转基因育种提供依据。©2022朋友科学出版社
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引用次数: 0
Interference of Herbicides in Association of Diazotrophic Bacterium Nitrospirillum amazonense and Sugarcane Pre-Sprouted Seedlings 除草剂对重氮营养细菌亚马孙硝化螺旋菌与甘蔗预芽苗关联的干扰
Q2 Agricultural and Biological Sciences Pub Date : 2022-03-01 DOI: 10.17957/ijab/15.1914
Luana Carolina Gomes Jonck
Microbial inoculant containing cells of Nitrospirillum amazonense is a recent technology that has been used in association with pre-sprouted seedlings to sustainably increase the productivity of sugarcane. This study aimed to assess the sensitivity of the rhizobacterium N. amazonense to the herbicides imazapic and indaziflam and the effect of this inoculation and herbicide treatments on sugarcane pre-sprouted seedlings. The In vitro sensitivity of the N. amazonense to the herbicides was assessed using the minimum inhibitory concentration technique (first assay). In this research, we evaluated imazapic (200 g a.i. ha-1) and indaziflam (100 g a.i. ha-1) at five doses: recommended dose (1×D), twice the recommended dose (2×D), one and a half of the recommended dose (1.5×D), half the recommended dose (0.5×CD), a quarter of the recommended dose (0.25×CD) and control treatment. The sensitivity of N. amazonense to imazapic and indaziflam applied at commercial doses on autoclaved soil was assessed in the second assay. The bacterial population count was performed using the most probable number technique (McCrady Table). The third assay assessed five herbicide treatments (clomazone (720 g a.i. ha−1), imazapic (200 g a.i. ha−1), tebuthiuron (800 g a.i. ha−1), indaziflam (75 g a.i. ha−1), sulfentrazone (800 g a.i. ha−1) and control without herbicide) applied in pre-planting of pre-sprouted seedlings of the variety RB 966928 in the presence and absence of the inoculant N. amazonense. The results showed that the presence of indaziflam did not interfere with the In vitro growth of the bacterium N. amazonense, regardless of the dose. Imazapic caused significant inhibition of bacterial In vitro growth from the recommended dose (200 g a.i. ha-1). The N. amazonense count in the soil of treatments that received indaziflam and imazapic application did not differ compared to the soil without herbicide. The pre-sprouted seedlings of the variety RB966928 showed high sensitivity to the herbicide imazapic, regardless of N. amazonense inoculation. Clomazone, tebuthiuron, and sulfentrazone did not interfere with the growth-promoting effect of N. amazonense. The results showed that the recommended dose of the herbicides tested does not impair the growth promoting effect of N. amazonense, and the inoculation of the pre-sprouted seedlings does not alter their sensitivity to herbicides, although the selectivity of the seedlings is differential among herbicides. Therefore, it may be concluded that the combined use of these technologies is a viable alternative to increase sugarcane productivity in a more sustainable way. © 2022 Friends Science Publishers
含有亚马逊氮螺菌细胞的微生物接种剂是一项最新技术,已与发芽前的幼苗结合使用,以可持续地提高甘蔗的生产力。本研究旨在评估根际细菌N.amazonese对除草剂imazapic和indaziflam的敏感性,以及这种接种和除草剂处理对甘蔗发芽前幼苗的影响。使用最小抑制浓度技术(第一次测定)评估了N.amazonese对除草剂的体外敏感性。在这项研究中,我们评估了五种剂量的伊玛扎匹克(200 g a.i.ha-1)和吲唑福林(100 g a.i.ha-1):推荐剂量(1×D)、推荐剂量(2×D)的两倍、推荐剂量的1.5×D的1.5倍、推荐量的0.5×CD的一半、推荐剂量四分之一(0.25×CD)和对照治疗。在第二次测定中评估了N.amazonese对在高压灭菌土壤上以商业剂量施用的imazapic和indaziflam的敏感性。使用最可能数技术(McCrady表)进行细菌种群计数。第三次试验评估了在有无接种剂N.amazonese的情况下,在品种RB 966928的预发芽幼苗的预种植中应用的五种除草剂处理(氯马宗(720 g a.i.ha−1)、伊玛扎匹克(200 g a.i.ha−1。结果表明,无论剂量如何,吲唑福明的存在都不会干扰细菌N.amazonese的体外生长。Imazapic在推荐剂量(200 g a.i.ha-1)下对细菌体外生长产生显著抑制。与未使用除草剂的土壤相比,使用吲唑福明和伊玛扎普处理的土壤中亚马逊N.amazonese含量没有差异。品种RB966928的预发芽幼苗对除草剂伊玛扎匹克表现出高敏感性,无论是否接种亚马逊农杆菌。Clomazone、tebuthiuron和sulfuentrazone不干扰N.amazonese的促生长作用。结果表明,推荐剂量的除草剂不会削弱亚马逊宁的促生长作用,接种预发芽幼苗不会改变其对除草剂的敏感性,尽管不同除草剂对幼苗的选择性不同。因此,可以得出结论,综合使用这些技术是以更可持续的方式提高甘蔗生产力的可行替代方案。©2022 Friends Science出版社
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引用次数: 0
Age-Stage, Two-Sex Life Table Study of the Effects of Sub-Lethal Concentrations of Novaluron on Earias vittella (Lepidoptera: Noctuidae) 异氰脲亚致死浓度对褐家鼠(鳞翅目:夜蛾科)影响的年龄、阶段、两性生命表研究
Q2 Agricultural and Biological Sciences Pub Date : 2022-03-01 DOI: 10.17957/ijab/15.1911
D. Khan
Earias vittella (F) is an important insect pest of cotton (Gossypium hirsutum) and okra (Abelmoschus esculentus) in Pakistan. The current study was carried out to explore the effects of sub-lethal concentrations of novaluron on the life table parameters of the pest. Bioassays were performed to assess the sub-lethal concentrations (LC20 and LC50) of the novaluron and its effects on the demographic parameters of the E. vittella. Age-stage, two-sex life table theory was applied to interpret the data for population parameters of E. vitella. In the current study, the LC20 and LC50 were calculated as 2.224 ppm and 9.837 ppm, respectively. The results showed that in novaluron treated samples rates of all biological parameters decreased whereas the larval, pupal period and mean generation time were increased. The intrinsic rate of increase remained high in control as 0.166 d-1 in comparison with LC50 as 0.128 d-1. The net reproductive rate ranged from 94.542 offsprings per individual (control) to 61.228 offsprings per individual (LC50). Fecundity was dropped in insects treated with sub-lethal concentrations from 330.9 eggs per female (control) to 238.11 eggs per female (LC50). This study revealed that the sub-lethal concentrations of novaluron significantly decreased the biological rate of E. vitella under laboratory conditions and suggests that such doses should be practiced in the fields for proper integrated pest management strategies. © 2022 Friends Science Publishers
卵圆螟(Earias vittella, F)是巴基斯坦棉花和秋葵的重要害虫。本研究旨在探讨亚致死浓度诺伐隆对害虫生命表参数的影响。采用生物测定法测定了新伐隆的亚致死浓度(LC20和LC50)及其对维氏乳杆菌种群参数的影响。应用年龄阶段、两性生命表理论解释了黄颡鱼种群参数数据。本研究计算LC20和LC50分别为2.224 ppm和9.837 ppm。结果表明:新伐威龙处理后,各生物指标率均降低,幼虫量、蛹期和平均产卵时间均增加;对照组的内在增长率为0.166 d-1,而LC50为0.128 d-1。净繁殖率在94.542 ~ 61.228之间(LC50)。亚致死浓度处理后,产卵量由330.9个/雌(对照)降至238.11个/雌(LC50)。本研究表明,在实验室条件下,亚致死浓度的诺伐威龙显著降低了牛肠杆菌的生物率,并建议在田间实施该剂量,以采取适当的害虫综合治理策略。©2022朋友科学出版社
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引用次数: 1
Oak gall extract: Molecular docking of wound healing and control of the skin pathogens Staphylococcus aureus and Candida albicans 橡树胆提取物:伤口愈合的分子对接和皮肤病原体金黄色葡萄球菌和白色念珠菌的控制
Q2 Agricultural and Biological Sciences Pub Date : 2022-03-01 DOI: 10.17957/ijab/15.1912
Shymaa A Elbuckley
Staphylococcus aureus and Candida albicans are common pathogens causing some health problems, however, the increase of their resistance to variety of medications increases the demand of searching for new antimicrobial agents. In this regard, the crude extract of Quercus infectoria (QIE) was verified as active antimicrobial with MBC of 1 mg/mL and MFC 10 mg/mL against S. aureus and C. albicans, respectively. SEM imaging of QIE-Treated-S. aureus and C. albicans cells showed fully lysed shrunk pathogen cells after 12 h. QIE showed good efficacy as potent anti-S. aureus and anti-C. albicans agents using Extract-Treated Cotton-Textiles. QIE ointment formulation showed faster rate of wound and burn healing in mice, with skin tissue development, at the 5th day, as compared to untreated control. A high significant wound closure (from 10 to 0 mm) and burn healing (from 25 to 2.3 mm) occurred after 6 days of treatment. Molecular docking predicted that ten major components in QIE namely (G-gallayol, Isocryptomerin, 10.7-methyl-3-hydroxymethylene-4,5,6,7,8-pentahydrox-h-thalene, Syringic acid, Gallotannic acid, Tannic acid, Pentagalloylglucose 1,β-sitosterol, Methyl oleanate, and Amentoflavone hexamethyl ether) are highly integrated in healing by promoting cell proliferation, keratinocyte migration, inhibiting collagenase, converting prothrombin to thrombin, increasing collagens function, enhancing immunity and DNA repair enzymes, as well as reducing inflammation. The combination of more than one bioactive compound in the extract and their synergetic action recommend the usage of QIE as effective topical applications for healing and skin disinfection. © 2022 Friends Science Publishers
金黄色葡萄球菌和白色念珠菌是引起一些健康问题的常见病原体,然而,它们对各种药物耐药性的增加增加了寻找新的抗菌剂的需求。在这方面,感染Quercus infectioria(QIE)的粗提取物被证实分别具有1mg/mL的MBC和10mg/mL的MFC对金黄色葡萄球菌和白色念珠菌具有活性抗菌作用。QIE-Treated-S的SEM成像。金黄色葡萄球菌和白色念珠菌细胞在12小时后显示出完全裂解的萎缩病原体细胞。金黄色葡萄球菌和抗C。使用经提取物处理的棉纺织品的白色念珠菌制剂。与未经治疗的对照组相比,QIE软膏制剂在第5天显示出小鼠的伤口和烧伤愈合速度更快,皮肤组织发育也更快。治疗6天后出现高度显著的伤口闭合(从10到0mm)和烧伤愈合(从25到2.3mm)。分子对接预测,QIE中的十种主要成分,即G-gallayol、异隐蛋白、10.7-甲基-3-羟基亚甲基-4,5,6,7,8-五氢x-h-thalene、丁香酸、Gallotaninic acid、Tannic acid、Pentagaloyl glucose 1、β-谷甾醇、油酸甲酯和Amentoflavone hexamethyl ether,通过促进细胞增殖、角质形成细胞迁移,抑制胶原酶,将凝血酶原转化为凝血酶,增加胶原蛋白功能,增强免疫力和DNA修复酶,以及减少炎症。提取物中一种以上生物活性化合物的组合及其协同作用建议将QIE用作治疗和皮肤消毒的有效局部应用。©2022 Friends Science出版社
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引用次数: 1
Mineralogically Well Characterized Bentonite Sources Controlled Aflatoxin Contamination in Poultry 矿物学特征良好的膨润土源控制家禽黄曲霉毒素污染
Q2 Agricultural and Biological Sciences Pub Date : 2022-03-01 DOI: 10.17957/ijab/15.1921
Ahmad Masood Khan
Aflatoxin in poultry feed reduces production, and is a health hazard to the consumers. Its toxicity is reduced through addition of clays. Evaluation of the country’s bentonites may promote their use as toxin binder. After detailed mineralogical and aflatoxin adsorption characteristics of large number of indigenous quarries effectiveness of two bentonites was determined through feeding trial. Bentonites from Bhimber and Dina quarries were applied in three replications at 1 and 2% (w/w) with positive and negative controls to a feed containing 250 μg kg-1 aflatoxin B1. Ten birds initially fed on clean starter feed for two weeks, fed on the experimental feeds and slaughtered after recording live weight on day 35 and dress weight along with internal organs weight were recorded with any apparent disorders. While addition of the bentonites had no detrimental effect, aflatoxin contamination had the lowest body weight and weight gain rate, reduced feed intake and feed conversion ratio with apparent disordered liver. The live body weight and weight gain rate increased with 2% bentonite addition to the contaminated feed from both the sources and was statistically similar to the clean feed though the values for these parameters were highest when the addition was to the clean feed. A 42% reduction occurred in weight gain without smectite addition in the toxin feed. The toxin feed had the lowest absolute liver and heart weight with dark coloration. The bentonites use in feed industry may reduce aflatoxin incidences at the application rate of 2%. © 2021 Friends Science Publishers
家禽饲料中的黄曲霉毒素降低了产量,对消费者的健康构成危害。其毒性通过添加粘土而降低。对该国膨润土的评估可能会促进其作为毒素粘合剂的使用。经过详细的矿物学和黄曲霉毒素吸附特性研究,通过投料试验确定了两种膨润土的有效性。将Bhimber和Dina采石场的膨润土以1%和2%(w/w)的浓度进行三次重复施用,阳性和阴性对照施用于含有250μg kg-1黄曲霉毒素B1的饲料中。10只最初用清洁的起始饲料喂养两周的鸟,用实验饲料喂养,并在记录第35天的活重和内脏重量后屠宰,记录下任何明显的疾病。虽然添加膨润土没有有害影响,但黄曲霉毒素污染的体重和增重率最低,饲料摄入量和饲料转化率降低,肝脏明显紊乱。在两种来源的污染饲料中添加2%的膨润土后,活体重和增重率增加,并且在统计上与清洁饲料相似,尽管当添加到清洁饲料中时,这些参数的值最高。在毒素饲料中不添加蒙脱石的情况下,体重增加减少了42%。毒素饲料的绝对肝脏和心脏重量最低,呈深色。饲料工业中使用的膨润土可以降低黄曲霉毒素的发生率,施用率为2%。©2021 Friends Science出版社
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引用次数: 0
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International Journal of Agriculture and Biology
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