The negative effects of water deficiency in sugarcane production caused by climate change on the productivity of sugarcane can be mitigated by drought tolerant varieties. A 14 × 2 factorial arrangement in completely randomised design replicated three times was used to screen 14 varieties for drought tolerance at the Zimbabwe Sugar Experiment Station (ZSAES). The first factor was the sugarcane varieties viz ZN1, ZN2, ZN3, ZN4, ZN5, ZN6, ZN7, ZN8, ZN9, ZN10, CP72–1312, NCo376, N14, and CP72–2086. The second factor comprised of two levels of irrigation, namely, well-watered (100% by volume) and water-deficit stressed (30% by volume). The parameters measured in this study which included tiller count, leaf SPAD index, total plant dry mass, photosynthetic rate, and leaf temperature were found not suitable for screening sugarcane for tolerance to water-deficit stress. Water-deficit stressed varieties ZN1, ZN8, ZN10, and N14 had the tallest stalks. Varieties CP72–2086, ZN2, ZN5, CP72–1312, ZN4, ZN6, and ZN9 were stunted, indicating that they were probably drought-sensitive. Leaf vapour pressure deficits of varieties ZN8, ZN10 and N14 were higher in water-stressed plants than in the well-watered ones. The vapour pressure deficit of well-watered NCo376 plants was higher than that of water-stressed plants. Furthermore, the stomatal conductance of water-stressed NCo376 plants was greater than that of the other varieties tested, showing more tolerance to drought. Based on stem height, stomatal conductance, vapour pressure deficit, transpiration rate and dry matter parameters measured in the present study, sugarcane varieties that are recommended to cane farmers in Zimbabwe when faced with drought are NCo376, ZN1, ZN8, ZN10 and ZN14.
{"title":"Screening of Sugarcane Varieties for Tolerance to Water Deficiency Using Containers","authors":"Misheck Chandiposha, G. E. Zharare, M. Nzima","doi":"10.1155/2023/5705785","DOIUrl":"https://doi.org/10.1155/2023/5705785","url":null,"abstract":"The negative effects of water deficiency in sugarcane production caused by climate change on the productivity of sugarcane can be mitigated by drought tolerant varieties. A 14 × 2 factorial arrangement in completely randomised design replicated three times was used to screen 14 varieties for drought tolerance at the Zimbabwe Sugar Experiment Station (ZSAES). The first factor was the sugarcane varieties viz ZN1, ZN2, ZN3, ZN4, ZN5, ZN6, ZN7, ZN8, ZN9, ZN10, CP72–1312, NCo376, N14, and CP72–2086. The second factor comprised of two levels of irrigation, namely, well-watered (100% by volume) and water-deficit stressed (30% by volume). The parameters measured in this study which included tiller count, leaf SPAD index, total plant dry mass, photosynthetic rate, and leaf temperature were found not suitable for screening sugarcane for tolerance to water-deficit stress. Water-deficit stressed varieties ZN1, ZN8, ZN10, and N14 had the tallest stalks. Varieties CP72–2086, ZN2, ZN5, CP72–1312, ZN4, ZN6, and ZN9 were stunted, indicating that they were probably drought-sensitive. Leaf vapour pressure deficits of varieties ZN8, ZN10 and N14 were higher in water-stressed plants than in the well-watered ones. The vapour pressure deficit of well-watered NCo376 plants was higher than that of water-stressed plants. Furthermore, the stomatal conductance of water-stressed NCo376 plants was greater than that of the other varieties tested, showing more tolerance to drought. Based on stem height, stomatal conductance, vapour pressure deficit, transpiration rate and dry matter parameters measured in the present study, sugarcane varieties that are recommended to cane farmers in Zimbabwe when faced with drought are NCo376, ZN1, ZN8, ZN10 and ZN14.","PeriodicalId":13844,"journal":{"name":"International Journal of Agronomy","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41573492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Hamane, Anass El yemlahi, M. Hassani Zerrouk, O. El Galiou, A. Laglaoui, M. Bakkali, A. Arakrak
Legumes, native to the Mediterranean, harbor reservoirs of endophytes that help plants adapt to various environmental stresses. The current study was carried out to evaluate the plant growth characteristics and antifungal activity of root nodule endophytes as biocontrol agents and plant growth promoters. Eleven bacterial endophytes isolated from root nodules of Sulla flexuosa L. grown in Northwest Morocco were assessed for their plant growth-promoting (PGP), and antifungal properties. Four endophytic bacteria were selected for their efficiency in solubilizing inorganic phosphate. The selected strains were positive for more than 2 PGP traits, including indole acetic acid, ACC deaminase, siderophore, and ammonia production. The screening for lytic enzyme production revealed that all strains were capable of producing chitinase, cellulase, catalase, and protease, while the secretion of amylase and urease was not detected. The HFB11 was the only strain incapable of producing pectinase. In vitro experiments revealed the strains’ potential to withstand salt and drought stresses by being able to grow in high concentrations of NaCl and PEG. Based on 16S rRNA gene sequencing, the strains were identified as Enterobacter and Serratia. The antagonistic activity of the strains against Botrytis cinerea, Aspergillus ochraceus, and Fusarium oxysporum was detected and they were shown to inhibit the fungal growth with various percentages. The highest percentage of inhibition was observed for HFB3 against B. cinerea with 50% inhibition followed by HFB8 which was able to inhibit 47% of F. oxysporum’s growth. In contrast, a weak inhibition was observed against A. ochraceus. All these findings indicate that the chosen endophytes, halotolerant Serratia inhibens HFB8 and Enterobacter hormaechei HFB11, might be used as candidates for effective biocontrol and growth promotion of legumes.
{"title":"Plant Growth Promotion and Biocontrol Potentiality of Endophytes Isolated from Root Nodules of Sulla flexuosa L. Plants","authors":"S. Hamane, Anass El yemlahi, M. Hassani Zerrouk, O. El Galiou, A. Laglaoui, M. Bakkali, A. Arakrak","doi":"10.1155/2023/2451806","DOIUrl":"https://doi.org/10.1155/2023/2451806","url":null,"abstract":"Legumes, native to the Mediterranean, harbor reservoirs of endophytes that help plants adapt to various environmental stresses. The current study was carried out to evaluate the plant growth characteristics and antifungal activity of root nodule endophytes as biocontrol agents and plant growth promoters. Eleven bacterial endophytes isolated from root nodules of Sulla flexuosa L. grown in Northwest Morocco were assessed for their plant growth-promoting (PGP), and antifungal properties. Four endophytic bacteria were selected for their efficiency in solubilizing inorganic phosphate. The selected strains were positive for more than 2 PGP traits, including indole acetic acid, ACC deaminase, siderophore, and ammonia production. The screening for lytic enzyme production revealed that all strains were capable of producing chitinase, cellulase, catalase, and protease, while the secretion of amylase and urease was not detected. The HFB11 was the only strain incapable of producing pectinase. In vitro experiments revealed the strains’ potential to withstand salt and drought stresses by being able to grow in high concentrations of NaCl and PEG. Based on 16S rRNA gene sequencing, the strains were identified as Enterobacter and Serratia. The antagonistic activity of the strains against Botrytis cinerea, Aspergillus ochraceus, and Fusarium oxysporum was detected and they were shown to inhibit the fungal growth with various percentages. The highest percentage of inhibition was observed for HFB3 against B. cinerea with 50% inhibition followed by HFB8 which was able to inhibit 47% of F. oxysporum’s growth. In contrast, a weak inhibition was observed against A. ochraceus. All these findings indicate that the chosen endophytes, halotolerant Serratia inhibens HFB8 and Enterobacter hormaechei HFB11, might be used as candidates for effective biocontrol and growth promotion of legumes.","PeriodicalId":13844,"journal":{"name":"International Journal of Agronomy","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42281012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The production of soybean is restricted in sub-Saharan Africa by several stress conditions, including drought because its production is exclusively rain-fed. Identifying drought resistant varieties is of paramount importance. Thus, the objectives of this work were to (i) evaluate the effect of polyethylene glycol 6000 (PEG6000) on soybean at the seedling stage, (ii) determine the root system architecture and physiological characters to water deficit stress, and (iii) establish the correlation among the quantitative variables responsible for drought tolerance in soybean varieties. Twenty soybean accessions (G1 to G20) were subjected to 10% PEG6000 concentration at seedling stages under a controlled environment using a randomized complete block design with 3 replicates. Vegetative growth data were collected. Highly significant differences P <