Pub Date : 2020-01-15DOI: 10.3760/CMA.J.ISSN.1673-4378.2020.01.009
Yuchao Wu, Wu Yinghui, L. Zhong, Tao Huang
Objective To evaluate the use of gabapentin in preemptive analgesia for adenotonsillectomy in children with obstructive sleep apnea hypopnea syndrome (OSAS). Methods Eighty OSAHS patients, American Society of Anesthesiologists (ASA)Ⅰ or Ⅱ , undergoing adenotonsillectomy were divided into two groups, according to a random number table:an observation group or a control group, with 40 patients in each group. Patients in the observation group were orally administered with gabapentin 30 mg/kg 3 h before entry in the operation room. In the control group, placebo was given 3 h before entry in the room. All patients received patient controlled intravenous analgesia (PCIA) with fentanyl, where 8 μg/kg fentanyl was diluted to 50 ml. The initial dose was 4 ml, without background dose, and the bolus was 2 ml, with a lockout time of 30 min. The bolus would be adjusted to 3 ml, if analgesia was inadequate 2 h later. Those with vomiting were intravenously injected with 0.1 mg/kg granisetron. The Children's and Infants Postoperative Pain Scale (CHIPPS) scores and the consumption of fentanyl were recorded 1, 2, 4, 8, 16 h and 24 h after surgery. The adverse reactions of both groups were recorded. Results The CHIPPS scores 1, 2, 4, 8, 16 h and 24 h after surgery and fentanyl doses were significantly lower in the observation group than those in the control group (P<0.05). Patients in the observation group reported less incidences of nausea, vomiting, anxiety and itching than the control (P<0.05). Conclusions The use of gabapentin in preemptive analgesia for adenotonsillectomy in children with OSAS can effectively relieve postoperative pain and reduce the consumption of opioids after operation. Key words: Gabapentin; Obstructive sleep apnea hypopnea syndrome; Preemptive analgesia; Postoperative analgesia; Children
{"title":"Clinical observation of preemptive analgesia with gabapentin for adenotonsillectomy in children with obstructive sleep apnea hypopnea syndrome","authors":"Yuchao Wu, Wu Yinghui, L. Zhong, Tao Huang","doi":"10.3760/CMA.J.ISSN.1673-4378.2020.01.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4378.2020.01.009","url":null,"abstract":"Objective \u0000To evaluate the use of gabapentin in preemptive analgesia for adenotonsillectomy in children with obstructive sleep apnea hypopnea syndrome (OSAS). \u0000 \u0000 \u0000Methods \u0000Eighty OSAHS patients, American Society of Anesthesiologists (ASA)Ⅰ or Ⅱ , undergoing adenotonsillectomy were divided into two groups, according to a random number table:an observation group or a control group, with 40 patients in each group. Patients in the observation group were orally administered with gabapentin 30 mg/kg 3 h before entry in the operation room. In the control group, placebo was given 3 h before entry in the room. All patients received patient controlled intravenous analgesia (PCIA) with fentanyl, where 8 μg/kg fentanyl was diluted to 50 ml. The initial dose was 4 ml, without background dose, and the bolus was 2 ml, with a lockout time of 30 min. The bolus would be adjusted to 3 ml, if analgesia was inadequate 2 h later. Those with vomiting were intravenously injected with 0.1 mg/kg granisetron. The Children's and Infants Postoperative Pain Scale (CHIPPS) scores and the consumption of fentanyl were recorded 1, 2, 4, 8, 16 h and 24 h after surgery. The adverse reactions of both groups were recorded. \u0000 \u0000 \u0000Results \u0000The CHIPPS scores 1, 2, 4, 8, 16 h and 24 h after surgery and fentanyl doses were significantly lower in the observation group than those in the control group (P<0.05). Patients in the observation group reported less incidences of nausea, vomiting, anxiety and itching than the control (P<0.05). \u0000 \u0000 \u0000Conclusions \u0000The use of gabapentin in preemptive analgesia for adenotonsillectomy in children with OSAS can effectively relieve postoperative pain and reduce the consumption of opioids after operation. \u0000 \u0000 \u0000Key words: \u0000Gabapentin; Obstructive sleep apnea hypopnea syndrome; Preemptive analgesia; Postoperative analgesia; Children","PeriodicalId":13847,"journal":{"name":"国际麻醉学与复苏杂志","volume":"41 1","pages":"39-42"},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48534792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-15DOI: 10.3760/CMA.J.ISSN.1673-4378.2020.01.025
Ya-jun Bai
With the development of surgical technology, more and more infants and pregnant women require surgery under general anesthesia. The neurotoxic effects of general anesthetics on late pregnancy and the development of infants and babies and related mechanisms have always been a hot topic. The excitotoxicity of γ-aminobutyric acid A (GABAA) receptor is one of the possible mechanisms involved in the neurotoxic effects of general anesthetics on developmental neurons. This review is designed to investigate the possible mechanism of GABAA receptor and its signaling pathways in the neurotoxic effects of general anesthetics on developmental neurons. Meanwhile, in-depth study and understanding of the relationship between GABAA receptor and its upstream and downstream signal pathways, especially the with-no-lysine (WNKs)-SPS1-related proline/alanine-rich kinase (SPAK)/oxidative stress-responsive 1 protein (OSR1) signal pathway, and the neurotoxic effects of general anesthetics on developmental neurons is done, so as to provide new ideas and targets for reducing the neurotoxicity of general anesthetics in the future. Key words: General anesthetics; Developmental phase; Neuron; Neurotoxicity; γ-aminobutyric acid A receptor; With-no-lysine-SPS1-related proline/alanine-rich kinase/oxidative stress-responsive 1 protein signal pathway
{"title":"Neurotoxic effects of γ-aminobutyric acid A receptor and its signal pathways and general anesthetics on developmental neurons","authors":"Ya-jun Bai","doi":"10.3760/CMA.J.ISSN.1673-4378.2020.01.025","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4378.2020.01.025","url":null,"abstract":"With the development of surgical technology, more and more infants and pregnant women require surgery under general anesthesia. The neurotoxic effects of general anesthetics on late pregnancy and the development of infants and babies and related mechanisms have always been a hot topic. The excitotoxicity of γ-aminobutyric acid A (GABAA) receptor is one of the possible mechanisms involved in the neurotoxic effects of general anesthetics on developmental neurons. This review is designed to investigate the possible mechanism of GABAA receptor and its signaling pathways in the neurotoxic effects of general anesthetics on developmental neurons. Meanwhile, in-depth study and understanding of the relationship between GABAA receptor and its upstream and downstream signal pathways, especially the with-no-lysine (WNKs)-SPS1-related proline/alanine-rich kinase (SPAK)/oxidative stress-responsive 1 protein (OSR1) signal pathway, and the neurotoxic effects of general anesthetics on developmental neurons is done, so as to provide new ideas and targets for reducing the neurotoxicity of general anesthetics in the future. \u0000 \u0000 \u0000Key words: \u0000General anesthetics; Developmental phase; Neuron; Neurotoxicity; γ-aminobutyric acid A receptor; With-no-lysine-SPS1-related proline/alanine-rich kinase/oxidative stress-responsive 1 protein signal pathway","PeriodicalId":13847,"journal":{"name":"国际麻醉学与复苏杂志","volume":"41 1","pages":"116-120"},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44812222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-15DOI: 10.3760/CMA.J.ISSN.1673-4378.2020.01.021
Qiqi Xu, Chen Liu, Fangfang Zhang, Zijian Zhao, Yao Chen, Yuan Han, Junli Cao
Chronic pain is one of the important factors that reduces the quality of life in breast cancer patients. With the development of biopsychosocial models of pain, the role of psychosocial factors has gradually attracted the attention of researchers. This article summarizes the latest studies concerning the psychosocial factors of breast cancer patients, reviews the psychosocial risk factors and protective factors related to chronic pain after breast cancer surgery, and introduces physiological mechanisms by which pain is closely associated with social psychology. At last, the psychotherapy currently used in clinical practice were mentioned as well. It is expected that clinicians pay more attention to the abnormal psychological condition of breast cancer patients, so as to reduce the occurrence of chronic pain and improve the overall prognosis of patients by adopting active and effective psychological interventions. Key words: Breast cancer; Chronic pain; Psychosocial; Psychotherapy
{"title":"Psychosocial factors and chronic pain after breast cancer surgery","authors":"Qiqi Xu, Chen Liu, Fangfang Zhang, Zijian Zhao, Yao Chen, Yuan Han, Junli Cao","doi":"10.3760/CMA.J.ISSN.1673-4378.2020.01.021","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4378.2020.01.021","url":null,"abstract":"Chronic pain is one of the important factors that reduces the quality of life in breast cancer patients. With the development of biopsychosocial models of pain, the role of psychosocial factors has gradually attracted the attention of researchers. This article summarizes the latest studies concerning the psychosocial factors of breast cancer patients, reviews the psychosocial risk factors and protective factors related to chronic pain after breast cancer surgery, and introduces physiological mechanisms by which pain is closely associated with social psychology. At last, the psychotherapy currently used in clinical practice were mentioned as well. It is expected that clinicians pay more attention to the abnormal psychological condition of breast cancer patients, so as to reduce the occurrence of chronic pain and improve the overall prognosis of patients by adopting active and effective psychological interventions. \u0000 \u0000 \u0000Key words: \u0000Breast cancer; Chronic pain; Psychosocial; Psychotherapy","PeriodicalId":13847,"journal":{"name":"国际麻醉学与复苏杂志","volume":"41 1","pages":"93-97"},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47388704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-15DOI: 10.3760/CMA.J.ISSN.1673-4378.2020.01.016
Xiaoli Wu, Qiang Li, Yue Li
Sedation in gastrointestinal endoscopy is an important requirement for safe and comfortable medical treatment. At present, the important steps in sedation treatment of gastrointestinal examination include standardization of sedation depth standards; on the basis of preoperative evaluation of patients and the requirements for sedation depth, sedation/anaesthesia staff are selected to monitor indicators and determine individualized sedation/analgesic regimen. This is very important for achieving programmatic sedation. Sedative treatment in gastrointestinal surgery can ensure patient safety, and require more cost-effectiveness and comfort. Key words: Sedation; Sedative drugs; Anesthesia; Monitoring; Gastrointestinal endoscopy
{"title":"New advances in sedation/anesthesia in the diagnosis and treatment of gastrointestinal endoscopy","authors":"Xiaoli Wu, Qiang Li, Yue Li","doi":"10.3760/CMA.J.ISSN.1673-4378.2020.01.016","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4378.2020.01.016","url":null,"abstract":"Sedation in gastrointestinal endoscopy is an important requirement for safe and comfortable medical treatment. At present, the important steps in sedation treatment of gastrointestinal examination include standardization of sedation depth standards; on the basis of preoperative evaluation of patients and the requirements for sedation depth, sedation/anaesthesia staff are selected to monitor indicators and determine individualized sedation/analgesic regimen. This is very important for achieving programmatic sedation. Sedative treatment in gastrointestinal surgery can ensure patient safety, and require more cost-effectiveness and comfort. \u0000 \u0000 \u0000Key words: \u0000Sedation; Sedative drugs; Anesthesia; Monitoring; Gastrointestinal endoscopy","PeriodicalId":13847,"journal":{"name":"国际麻醉学与复苏杂志","volume":"41 1","pages":"71-75"},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43362426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-15DOI: 10.3760/CMA.J.ISSN.1673-4378.2020.01.022
Ke Ding, Yuan Han, Zhenfeng Zhang, Han Liu, Cao Jun-li
Alzheimer's disease (AD) is a common chronic neurodegenerative disease in the central nervous system. Its pathogenesis and mechanism have not been identified. Studies indicate that an individual carrying certain genotypes of susceptibility gene has a higher risk of AD. With the completion of the Human Genome Project and the development of genome-wide association studies(GWAS), a series of susceptibility genes associated with late-onset AD (LOAD) have been discovered. In this paper, the development of susceptibility genes associated with LOAD is reviewed, so as to provide readers with current research progress and clues for future research. Key words: Alzheimer's disease; Susceptibility gene; Neurodegenerative diseases
{"title":"Research progresses in the genetic susceptibility of late-onset Alzheimer's disease","authors":"Ke Ding, Yuan Han, Zhenfeng Zhang, Han Liu, Cao Jun-li","doi":"10.3760/CMA.J.ISSN.1673-4378.2020.01.022","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4378.2020.01.022","url":null,"abstract":"Alzheimer's disease (AD) is a common chronic neurodegenerative disease in the central nervous system. Its pathogenesis and mechanism have not been identified. Studies indicate that an individual carrying certain genotypes of susceptibility gene has a higher risk of AD. With the completion of the Human Genome Project and the development of genome-wide association studies(GWAS), a series of susceptibility genes associated with late-onset AD (LOAD) have been discovered. In this paper, the development of susceptibility genes associated with LOAD is reviewed, so as to provide readers with current research progress and clues for future research. \u0000 \u0000 \u0000Key words: \u0000Alzheimer's disease; Susceptibility gene; Neurodegenerative diseases","PeriodicalId":13847,"journal":{"name":"国际麻醉学与复苏杂志","volume":"41 1","pages":"98-104"},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43465719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-15DOI: 10.3760/CMA.J.ISSN.1673-4378.2020.01.015
R. Tang
Ventilator induced lung injury (VILI) is caused or aggravated by invasive mechanical ventilation. With the continuous exploration of the pathogenesis of VILI, increasing studies have been carried out to explain the strategies of lung protective ventilation that can alleviate VILI injury. Currently, how to determine the parameters of individualized lung protective ventilation is an urgent clinical problem to be solved. Recently proposed driving pressure-guided lung protective ventilation strategy can significantly improve patient prognosis, and is expected to become a new type of lung protection strategy in perioperative application. This review is to analyze the concept and meaning of driving pressure, summarize its application in perioperative period, so as to provide new ideas for lung protective ventilation strategy. Key words: Lung; Mechanical ventilation; Driving pressure; Protective ventilation strategy; Complication
{"title":"Progresses in driving pressure-guided pulmonary protective ventilation strategy in perioperative period","authors":"R. Tang","doi":"10.3760/CMA.J.ISSN.1673-4378.2020.01.015","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4378.2020.01.015","url":null,"abstract":"Ventilator induced lung injury (VILI) is caused or aggravated by invasive mechanical ventilation. With the continuous exploration of the pathogenesis of VILI, increasing studies have been carried out to explain the strategies of lung protective ventilation that can alleviate VILI injury. Currently, how to determine the parameters of individualized lung protective ventilation is an urgent clinical problem to be solved. Recently proposed driving pressure-guided lung protective ventilation strategy can significantly improve patient prognosis, and is expected to become a new type of lung protection strategy in perioperative application. This review is to analyze the concept and meaning of driving pressure, summarize its application in perioperative period, so as to provide new ideas for lung protective ventilation strategy. \u0000 \u0000 \u0000Key words: \u0000Lung; Mechanical ventilation; Driving pressure; Protective ventilation strategy; Complication","PeriodicalId":13847,"journal":{"name":"国际麻醉学与复苏杂志","volume":"41 1","pages":"67-70"},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47648394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-15DOI: 10.3760/CMA.J.ISSN.1673-4378.2020.01.007
Siyuan Liu, Binbin Wang, Jie Gao, Yongtao Gao
Objective To observe the effects of apneic oxygen insufflation (AOI) on the oxygenation and intra-alveolar pressure of patients with one lung ventilation (OLV). Methods Ninety patients undergoing open thoracotomy were enrolled, where 45 patients were divided into three groups according to a random number table: a control group (group A), a test 1 group (group B) and a test 2 group (group C), with 15 patients in each group; and another 45 patients were divided into three groups according to the pattern of double-lumen tubes: a 35 Fr group (group D), a 37 Fr group (group E), and a 39 Fr group (group F), with 15 patients in each group. During OLV, the non-ventilated lungs in groups A, B, and C were delivered with oxygen at a rate of 0, 3 and 5 L/min, respectively, and arterial blood samples were taken 1 min before OLV (T1), 30 min after the beginning of OLV (T2), and 60 min after the beginning of OLV (T3) for blood gas analysis, so as to calculate oxygenation index (OI), intrapulmonary shunt rate (Qs/Qt) and the satisfaction of surgeons towards collapsed lung. The intra-alveolar pressure at different oxygen flow rates (2-9 L/min) was measured in groups D, E and F after the beginning of OLV. Results OI decreased and Qs/Qt increased in groups A, B and C at T2 and T3 compared with those at T1 (P< 0.05). OI was remarkably higher in group B at T2 and higher in group C at T2 and T3 than those in group A, and Qs/Qt was significantly lower in group B at T2 and lower in group C at T2 and T3than those in group A (P<0.05). Compared with group B, group C presented remarkable increases in OI and significant decreases in Qs/Qt at T2 and T3 (P<0.05). The intra-alveolar pressure was lower in groups E and group F at different oxygen flow rates than that in group D (P<0.05); the intra-alveolar pressure was lower in group F at different oxygen flow rates than that in group E (P<0.05). Conclusions AOI at 5 L/min during OLV can maintain gas exchange to some extent in the alveoli, improve oxygenation and reduce Qs/Qt, which may be related to the intra-alveolar pressure produced by AOI in the operated lung. Key words: One lung ventilation; Apneic oxygen insufflation; Oxygenation index; Intra-alveolar pressure
{"title":"Effects of apneic oxygen insufflation on oxygenation and intra-alveolar pressure during one lung ventilation","authors":"Siyuan Liu, Binbin Wang, Jie Gao, Yongtao Gao","doi":"10.3760/CMA.J.ISSN.1673-4378.2020.01.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4378.2020.01.007","url":null,"abstract":"Objective \u0000To observe the effects of apneic oxygen insufflation (AOI) on the oxygenation and intra-alveolar pressure of patients with one lung ventilation (OLV). \u0000 \u0000 \u0000Methods \u0000Ninety patients undergoing open thoracotomy were enrolled, where 45 patients were divided into three groups according to a random number table: a control group (group A), a test 1 group (group B) and a test 2 group (group C), with 15 patients in each group; and another 45 patients were divided into three groups according to the pattern of double-lumen tubes: a 35 Fr group (group D), a 37 Fr group (group E), and a 39 Fr group (group F), with 15 patients in each group. During OLV, the non-ventilated lungs in groups A, B, and C were delivered with oxygen at a rate of 0, 3 and 5 L/min, respectively, and arterial blood samples were taken 1 min before OLV (T1), 30 min after the beginning of OLV (T2), and 60 min after the beginning of OLV (T3) for blood gas analysis, so as to calculate oxygenation index (OI), intrapulmonary shunt rate (Qs/Qt) and the satisfaction of surgeons towards collapsed lung. The intra-alveolar pressure at different oxygen flow rates (2-9 L/min) was measured in groups D, E and F after the beginning of OLV. \u0000 \u0000 \u0000Results \u0000OI decreased and Qs/Qt increased in groups A, B and C at T2 and T3 compared with those at T1 (P< 0.05). OI was remarkably higher in group B at T2 and higher in group C at T2 and T3 than those in group A, and Qs/Qt was significantly lower in group B at T2 and lower in group C at T2 and T3than those in group A (P<0.05). Compared with group B, group C presented remarkable increases in OI and significant decreases in Qs/Qt at T2 and T3 (P<0.05). The intra-alveolar pressure was lower in groups E and group F at different oxygen flow rates than that in group D (P<0.05); the intra-alveolar pressure was lower in group F at different oxygen flow rates than that in group E (P<0.05). \u0000 \u0000 \u0000Conclusions \u0000AOI at 5 L/min during OLV can maintain gas exchange to some extent in the alveoli, improve oxygenation and reduce Qs/Qt, which may be related to the intra-alveolar pressure produced by AOI in the operated lung. \u0000 \u0000 \u0000Key words: \u0000One lung ventilation; Apneic oxygen insufflation; Oxygenation index; Intra-alveolar pressure","PeriodicalId":13847,"journal":{"name":"国际麻醉学与复苏杂志","volume":"41 1","pages":"31-34"},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46083005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-15DOI: 10.3760/CMA.J.ISSN.1673-4378.2020.01.003
Guiping Xu, D. Hu, Xiaoli Wang, Xuan Zhao, Juan-juan Fu
Objective To observe the effect of nuclear factor-erythroid 2-related factor 2 (Nrf2) in diabetic rats preconditioning with resveratrol (Res) against myocardial ischemia/reperfusion injury (MI/RI). Methods Healthy adult male SD rats weighing 200- 220 g and aged from 4 to 5 months were fed with high-fat diet for 4 weeks and then intraperitoneally injected with streptozotocin (STZ) 30 mg/kg to establish a diabetic rat model. Thirty successfully modeled rats were divided into three groups according to a random number table (n=10): a sham operation group (a Sham group), a myocardial ischemia/reperfusion group (an MI/R group) and a myocardial ischemia/reperfusion+resveratrol group (an MI/R+Res group). A rat model of MI/RI was established by ligation of the left anterior descending coronary artery for 30 min before reperfusion for 120 min. In the MI/R+Res group, 15 mg/kg Res was intraperitoneally injected 7 d before establishment of the MI/RI model, once a day for 7 consecutive days. The Sham and MI/R groups were intraperitoneally injected with an equal volume of dimethyl sulfoxide (DMSO) and PBS mixture. Left ventricular ejection fraction (LVEF) and left ventricular short axis shortening (FS) were measured by B-mode ultrasonography. Five rats were sacrificed 120 min after reperfusion. Myocardial infarction size was measured by 2, 3, 5-triphenyl-2H-tetrazolium chloride (TTC) staining. Another five rats were sacrificed to collect blood samples from the abdominal aorta for measurement of lactate dehydrogenase (LDH), a marker of myocardial injury, and creatine kinase isoenzyme (CK-MB). Their myocardial tissues were collected to determine the levels of superoxide dismutase(SOD), malondialdehyde (MDA) and reactive oxygen species (ROS) by enzyme-linked immunosorbent assay (ELISA). The contents of Nrf2 were observed by immunohistochemistry. The expression of Nrf2, n-Nrf2 and heme oxygenase-1 (HO-1) were determined by Western blot. Results Compared with those in the Sham group, LDH, CK-MB concentration, ROS and MDA content in the MI/R and MI/R+ Res groups significantly increased while LVEF, FS, and SOD activity in the MI/R and MI/R+Res groups significantly decreased. Mean-while, the myocardial infarction sizes in the MI/R and MI/R+Res groups were increased, and the expression of Nrf2, n-Nrf2 and HO-1 protein was down-regulated (all P<0.05). Compared with those in the MI/R group, LDH, CK-MB concentration, ROS and MDA content significantly decreased in the MI/R+Res group, while LVEF, FS and SOD activity significantly increased in the MI/R+Res group. Also, in the MI/R+Res group, the myocardial infarction size decreased while the expression of Nrf2, n-Nrf2 and HO-1 protein increased (all P< 0.05). Conclusions Resveratrol preconditioning relieves myocardial injury in diabetic rats with ischemia/reperfusion, which may attribute to activation of Nrf2. Key words: Resveratrol; Transcription factor-related factor 2; Heme oxygenase-1; Diabetes; M
{"title":"Role of nuclear factor-erythroid 2-related factor 2 in diabetic rats with resveratrol preconditioning against myocardial isch- emia-reperfusion injury","authors":"Guiping Xu, D. Hu, Xiaoli Wang, Xuan Zhao, Juan-juan Fu","doi":"10.3760/CMA.J.ISSN.1673-4378.2020.01.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4378.2020.01.003","url":null,"abstract":"Objective \u0000To observe the effect of nuclear factor-erythroid 2-related factor 2 (Nrf2) in diabetic rats preconditioning with resveratrol (Res) against myocardial ischemia/reperfusion injury (MI/RI). \u0000 \u0000 \u0000Methods \u0000Healthy adult male SD rats weighing 200- 220 g and aged from 4 to 5 months were fed with high-fat diet for 4 weeks and then intraperitoneally injected with streptozotocin (STZ) 30 mg/kg to establish a diabetic rat model. Thirty successfully modeled rats were divided into three groups according to a random number table (n=10): a sham operation group (a Sham group), a myocardial ischemia/reperfusion group (an MI/R group) and a myocardial ischemia/reperfusion+resveratrol group (an MI/R+Res group). A rat model of MI/RI was established by ligation of the left anterior descending coronary artery for 30 min before reperfusion for 120 min. In the MI/R+Res group, 15 mg/kg Res was intraperitoneally injected 7 d before establishment of the MI/RI model, once a day for 7 consecutive days. The Sham and MI/R groups were intraperitoneally injected with an equal volume of dimethyl sulfoxide (DMSO) and PBS mixture. Left ventricular ejection fraction (LVEF) and left ventricular short axis shortening (FS) were measured by B-mode ultrasonography. Five rats were sacrificed 120 min after reperfusion. Myocardial infarction size was measured by 2, 3, 5-triphenyl-2H-tetrazolium chloride (TTC) staining. Another five rats were sacrificed to collect blood samples from the abdominal aorta for measurement of lactate dehydrogenase (LDH), a marker of myocardial injury, and creatine kinase isoenzyme (CK-MB). Their myocardial tissues were collected to determine the levels of superoxide dismutase(SOD), malondialdehyde (MDA) and reactive oxygen species (ROS) by enzyme-linked immunosorbent assay (ELISA). The contents of Nrf2 were observed by immunohistochemistry. The expression of Nrf2, n-Nrf2 and heme oxygenase-1 (HO-1) were determined by Western blot. \u0000 \u0000 \u0000Results \u0000Compared with those in the Sham group, LDH, CK-MB concentration, ROS and MDA content in the MI/R and MI/R+ Res groups significantly increased while LVEF, FS, and SOD activity in the MI/R and MI/R+Res groups significantly decreased. Mean-while, the myocardial infarction sizes in the MI/R and MI/R+Res groups were increased, and the expression of Nrf2, n-Nrf2 and HO-1 protein was down-regulated (all P<0.05). Compared with those in the MI/R group, LDH, CK-MB concentration, ROS and MDA content significantly decreased in the MI/R+Res group, while LVEF, FS and SOD activity significantly increased in the MI/R+Res group. Also, in the MI/R+Res group, the myocardial infarction size decreased while the expression of Nrf2, n-Nrf2 and HO-1 protein increased (all P< 0.05). \u0000 \u0000 \u0000Conclusions \u0000Resveratrol preconditioning relieves myocardial injury in diabetic rats with ischemia/reperfusion, which may attribute to activation of Nrf2. \u0000 \u0000 \u0000Key words: \u0000Resveratrol; Transcription factor-related factor 2; Heme oxygenase-1; Diabetes; M","PeriodicalId":13847,"journal":{"name":"国际麻醉学与复苏杂志","volume":"41 1","pages":"11-17"},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43839752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-15DOI: 10.3760/CMA.J.ISSN.1673-4378.2020.01.019
Nan Li, Shuangling Li, Dong-Xin Wang, Li Yang
Acute kidney injury (AKI) is a common complication after non-cardiac surgery with a variety of risk factors during perioperative period, resulting in adverse short and long-term outcomes of patients. Nowadays, early detection of injury in kidney is the first priori, development of novel biomarker making this possible. However, research in this field are mainly focused on cardiac surgery and ICU patients. In this article, it was reviewed the available literature on characteristics of promising AKI biomarkers that are currently the focus of preclinical and clinical investigations in non-cardiac surgery patients. These biomarkers include neutrophil gelatinase-associated lipocalin (NGAL), liver-type fatty acid-binding protein (L-FABP), insulin-like growth factor-binding protein 7 (IGFBP-7) and tissue inhibitor of metalloproteinase 2 (TIMP-2). It was then described the clinical performance of these biomarkers for diagnosis and prognostication of AKI. Finally, the study on biomarker guided interventional therapy for patients at high risk of postoperative AKI was reviewed. Novel biomarker will play a more important role in the diagnosis and treatment for AKI in patients after non-cardiac surgery. Key words: Non-cardiac surgery; Acute kidney injury; Biomarker; Early diagnosis; Prognosis
{"title":"Advances of biomarkers in acute kidney injury after non-cardiac surgery","authors":"Nan Li, Shuangling Li, Dong-Xin Wang, Li Yang","doi":"10.3760/CMA.J.ISSN.1673-4378.2020.01.019","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4378.2020.01.019","url":null,"abstract":"Acute kidney injury (AKI) is a common complication after non-cardiac surgery with a variety of risk factors during perioperative period, resulting in adverse short and long-term outcomes of patients. Nowadays, early detection of injury in kidney is the first priori, development of novel biomarker making this possible. However, research in this field are mainly focused on cardiac surgery and ICU patients. In this article, it was reviewed the available literature on characteristics of promising AKI biomarkers that are currently the focus of preclinical and clinical investigations in non-cardiac surgery patients. These biomarkers include neutrophil gelatinase-associated lipocalin (NGAL), liver-type fatty acid-binding protein (L-FABP), insulin-like growth factor-binding protein 7 (IGFBP-7) and tissue inhibitor of metalloproteinase 2 (TIMP-2). It was then described the clinical performance of these biomarkers for diagnosis and prognostication of AKI. Finally, the study on biomarker guided interventional therapy for patients at high risk of postoperative AKI was reviewed. Novel biomarker will play a more important role in the diagnosis and treatment for AKI in patients after non-cardiac surgery. \u0000 \u0000 \u0000Key words: \u0000Non-cardiac surgery; Acute kidney injury; Biomarker; Early diagnosis; Prognosis","PeriodicalId":13847,"journal":{"name":"国际麻醉学与复苏杂志","volume":"41 1","pages":"83-88"},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47351404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cerebral ischemia/reperfusion injury (CI/RI) is a common clinical pathophysiological phenomenon after thrombus recanalization in stroke patients. Its pathogenetic mechanism involves multiple steps, of which mitochondria play a key role as "energy stations". Cerebral ischemic stroke induces remarkable changes in the expression of microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in the brain, which indicates the association of miRNAs and lncRNAs of the complex pathological process after ischemic stroke. In addition, some related miRNAs and lncRNAs are found to participate in the development of CI/RI by regulating mitochondria. The current review is designed to summarize the effects of mitochondria on CI/RI, and the latest research findings about the involvement of miRNAs and lncRNAs to regulate CI/RI, so as to provide new targets and ideas for the prevention and treatment of CI/RI, as well as new thoughts and ideas for the prevention and treatment of CI/RI. Key words: Cerebral ischemia; Reperfusion injury; Mitochondria; MicroRNAs; Long non-coding RNAs
{"title":"Research progress in the effects of regulation of mitochondria by microRNAs and long non-coding RNAs on cerebral ischemia/reperfusion injury","authors":"Yanan Tang, Qian Zhang, Gaofeng Zhang, Huai-long Chen, Xiao-peng Sun, Fei Shi","doi":"10.3760/CMA.J.ISSN.1673-4378.2020.01.024","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4378.2020.01.024","url":null,"abstract":"Cerebral ischemia/reperfusion injury (CI/RI) is a common clinical pathophysiological phenomenon after thrombus recanalization in stroke patients. Its pathogenetic mechanism involves multiple steps, of which mitochondria play a key role as \"energy stations\". Cerebral ischemic stroke induces remarkable changes in the expression of microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in the brain, which indicates the association of miRNAs and lncRNAs of the complex pathological process after ischemic stroke. In addition, some related miRNAs and lncRNAs are found to participate in the development of CI/RI by regulating mitochondria. The current review is designed to summarize the effects of mitochondria on CI/RI, and the latest research findings about the involvement of miRNAs and lncRNAs to regulate CI/RI, so as to provide new targets and ideas for the prevention and treatment of CI/RI, as well as new thoughts and ideas for the prevention and treatment of CI/RI. \u0000 \u0000 \u0000Key words: \u0000Cerebral ischemia; Reperfusion injury; Mitochondria; MicroRNAs; Long non-coding RNAs","PeriodicalId":13847,"journal":{"name":"国际麻醉学与复苏杂志","volume":"41 1","pages":"111-115"},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45896254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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