Pub Date : 2023-04-15DOI: 10.18231/j.ijcbr.2023.008
P. Sagar, S Kumar, Neha Mala Krishna, Manish Kumar, J. Keshari
Breast cancer is the most frequent cancer among women. Prolactin (PRL) is a hormone secreted from anterior pituitary gland which stimulates cell proliferation, survival of cancer cells, its migration, invasion and angiogenesis. Breast cancer is strongly associated with raised plasma Prolactin level (PRL). The Prolactin behaves like Growth Hormone and its actions by the growth-promoting JAK/STAT pathway suggests its tumor-promoting effects.Ki-67 a Nuclear Protein is highest in Luminal B, Ki67 are not related to age but is correlated with tumor size. Fraction of Ki-67 positive tumor cells correlates with the clinical course of cancer cells. Hence, Ki- 67 can be an effective target in cancer therapy. Ki-67% and Serum Prolactin level may be considered a valuable biomarker in breast cancer patients and be used in treatment, follow-up and Prognosis.
{"title":"Evaluation of role of serum prolactin & ki-67 in different stages of breast cancer","authors":"P. Sagar, S Kumar, Neha Mala Krishna, Manish Kumar, J. Keshari","doi":"10.18231/j.ijcbr.2023.008","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.008","url":null,"abstract":"Breast cancer is the most frequent cancer among women. Prolactin (PRL) is a hormone secreted from anterior pituitary gland which stimulates cell proliferation, survival of cancer cells, its migration, invasion and angiogenesis. Breast cancer is strongly associated with raised plasma Prolactin level (PRL). The Prolactin behaves like Growth Hormone and its actions by the growth-promoting JAK/STAT pathway suggests its tumor-promoting effects.Ki-67 a Nuclear Protein is highest in Luminal B, Ki67 are not related to age but is correlated with tumor size. Fraction of Ki-67 positive tumor cells correlates with the clinical course of cancer cells. Hence, Ki- 67 can be an effective target in cancer therapy. Ki-67% and Serum Prolactin level may be considered a valuable biomarker in breast cancer patients and be used in treatment, follow-up and Prognosis.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85721509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.18231/j.ijcbr.2023.015
Arshi Anjum, S. Bagade, P. Boyella
: Monoclonal gammopathy include several clinical variants ranging from asymptomatic MGUS, asymptomatic smouldering myeloma, multiple myeloma to aggressive plasma cell leukemia. The characteristic property of myeloma cells is the production and secretion of M protein. Due to its diverse structure, no single test can identify M protein accurately in all patients. A simple, non-invasive combination of tests is necessary for the screening of monoclonal gammopathy. The present study aims to assess the diagnostic sensitivity of the biochemical tests used in the screening of monoclonal gammopathy.: This study was conducted retrospectively of the newly diagnosed cases of monoclonal gammopathy screened with SPE and ISUB/IT using sebia capillary electrophoresis and serum free light chain ratio using immunoturbidimetry method.: Out of the 142 patients included in the study, 120 had M band in SPE, 125 had monoclonal gammopathy by ISUB/IT and 121 patients had abnormal sFLCR. The diagnostic sensitivity obtained was 84.5% 88% and 85.2% for SPE, ISUB/IT and sFLCR respectively. Addition of sFLCR to SPE and ISUB/IT could identify 20 patients who had no abnormality in either of these tests. The combined sensitivity of SPE and ISUB/IT was 88%, while that of SPE and sFLCR and a combination of all the three tests was 98.6%. The simple, non-invasive, cost-effective screening panel of SPE plus sFLC ratio could be used as the initial screening method for patients with suspected monoclonal gammopathy with increased reliability.
{"title":"Comparision of the diagnostic sensitivity of biochemical tests in the screening of monoclonal gammopathy","authors":"Arshi Anjum, S. Bagade, P. Boyella","doi":"10.18231/j.ijcbr.2023.015","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.015","url":null,"abstract":": Monoclonal gammopathy include several clinical variants ranging from asymptomatic MGUS, asymptomatic smouldering myeloma, multiple myeloma to aggressive plasma cell leukemia. The characteristic property of myeloma cells is the production and secretion of M protein. Due to its diverse structure, no single test can identify M protein accurately in all patients. A simple, non-invasive combination of tests is necessary for the screening of monoclonal gammopathy. The present study aims to assess the diagnostic sensitivity of the biochemical tests used in the screening of monoclonal gammopathy.: This study was conducted retrospectively of the newly diagnosed cases of monoclonal gammopathy screened with SPE and ISUB/IT using sebia capillary electrophoresis and serum free light chain ratio using immunoturbidimetry method.: Out of the 142 patients included in the study, 120 had M band in SPE, 125 had monoclonal gammopathy by ISUB/IT and 121 patients had abnormal sFLCR. The diagnostic sensitivity obtained was 84.5% 88% and 85.2% for SPE, ISUB/IT and sFLCR respectively. Addition of sFLCR to SPE and ISUB/IT could identify 20 patients who had no abnormality in either of these tests. The combined sensitivity of SPE and ISUB/IT was 88%, while that of SPE and sFLCR and a combination of all the three tests was 98.6%. The simple, non-invasive, cost-effective screening panel of SPE plus sFLC ratio could be used as the initial screening method for patients with suspected monoclonal gammopathy with increased reliability.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81585848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.18231/j.ijcbr.2023.012
Preeclampsia is a human pregnancy specific multisystem disease of unknown etiology. It is a major cause of maternal mortality, morbidities, perinatal deaths, preterm birth and intrauterine growth restriction. It was aimed to find the role of Uric Acid, CRP and Nitric Oxide in preeclampsia by comparing their levels with normal pregnant females. As its etiology is unknown, so it’s early detection and its follow up is required to prevent maternal and fetal complicationsA case control study was conducted in the Department of Biochemistry and Department of Obstetrics and Gynaecology, UPUMS Medical College and Hospital, Saifai, Etawah, Uttar Pradesh, India. The study group includes 50 normal healthy pregnant females and 50 preeclampsia patients who were between 20 - 40 years of age and more than 20 weeks of gestation.There was significantly (p<0.0001) raised serum uric acid, C-reactive protein and decreased nitric oxide levels in preeclampsia patients as compared to normal healthy pregnant females.: It concludes that high serum levels of uric acid, C-reactive protein and low levels of nitric oxide shows their important role in pathogenesis of preeclampsia. Their serum levels can be considered as an indicative markers for disease and its severity of preeclampsia to prevent maternal and fetal complications.
{"title":"A comparison of serum levels of uric acid, c-reactive protein and nitric oxide in preeclampsia patients and normal healthy pregnant females","authors":"","doi":"10.18231/j.ijcbr.2023.012","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.012","url":null,"abstract":"Preeclampsia is a human pregnancy specific multisystem disease of unknown etiology. It is a major cause of maternal mortality, morbidities, perinatal deaths, preterm birth and intrauterine growth restriction. It was aimed to find the role of Uric Acid, CRP and Nitric Oxide in preeclampsia by comparing their levels with normal pregnant females. As its etiology is unknown, so it’s early detection and its follow up is required to prevent maternal and fetal complicationsA case control study was conducted in the Department of Biochemistry and Department of Obstetrics and Gynaecology, UPUMS Medical College and Hospital, Saifai, Etawah, Uttar Pradesh, India. The study group includes 50 normal healthy pregnant females and 50 preeclampsia patients who were between 20 - 40 years of age and more than 20 weeks of gestation.There was significantly (p<0.0001) raised serum uric acid, C-reactive protein and decreased nitric oxide levels in preeclampsia patients as compared to normal healthy pregnant females.: It concludes that high serum levels of uric acid, C-reactive protein and low levels of nitric oxide shows their important role in pathogenesis of preeclampsia. Their serum levels can be considered as an indicative markers for disease and its severity of preeclampsia to prevent maternal and fetal complications.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82587085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.18231/j.ijcbr.2023.007
R. Devi, Niangngaih Lian, T. O. Singh, Chungkham Rebika Devi
Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) causes coronavirus disease 2019 (COVID-19) with clinical outcome ranging from asymptomatic to severe disease, and even death to some. It posed a terrifying challenge to healthcare system worldwide. Several observational and clinical trials has reported that, Vitamin D deficiency has contributed to acute respiratory distress syndrome. Case fatality rate increases with age and comorbidities, both of which are associated with decreased Vitamin D level. Therefore, this study is done to study the prevalence of 25(OH)Vitamin D in RT-PCR positive COVID-19 cases and RT-PCR negative controls.This is a hospital based cross-sectional study conducted at Jawaharlal Nehru Institute of Medical Sciences, Manipur on 88 RT-PCR positive Covid-19 cases and 88 COVID-19 negative controls over a period of 2 years. Analysis of the sample was done by Liaison 25(OH) Vitamin D Total Chemiluminescence assay(CLIA).In this study, statistically significant (p-0.018) lower plasma 25(OH)Vitamin D level is seen in COVID-19 positive cases (median 28±20.47) when compared to Covid-19 negative controls(median 33.50±10.66). The number of 25(OH) Vitamin D deficient is higher in Covid-19 positive cases when compared to Covid-19 negative controls -46(52.3%) and 30(34%) respectively with a high statistically significant value (p-0.015).COVID-19 positive cases have higher tendency to have suboptimal plasma 25(OH) Vitamin D level which may contribute to the high hospitalization risk in COVID-19 infection. This finding is important as it can identify population at risk, and contribute to interventions in reducing the risk of hospitalization associated with COVID-19 infection.
{"title":"Vitamin D in COVID-19","authors":"R. Devi, Niangngaih Lian, T. O. Singh, Chungkham Rebika Devi","doi":"10.18231/j.ijcbr.2023.007","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.007","url":null,"abstract":"Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) causes coronavirus disease 2019 (COVID-19) with clinical outcome ranging from asymptomatic to severe disease, and even death to some. It posed a terrifying challenge to healthcare system worldwide. Several observational and clinical trials has reported that, Vitamin D deficiency has contributed to acute respiratory distress syndrome. Case fatality rate increases with age and comorbidities, both of which are associated with decreased Vitamin D level. Therefore, this study is done to study the prevalence of 25(OH)Vitamin D in RT-PCR positive COVID-19 cases and RT-PCR negative controls.This is a hospital based cross-sectional study conducted at Jawaharlal Nehru Institute of Medical Sciences, Manipur on 88 RT-PCR positive Covid-19 cases and 88 COVID-19 negative controls over a period of 2 years. Analysis of the sample was done by Liaison 25(OH) Vitamin D Total Chemiluminescence assay(CLIA).In this study, statistically significant (p-0.018) lower plasma 25(OH)Vitamin D level is seen in COVID-19 positive cases (median 28±20.47) when compared to Covid-19 negative controls(median 33.50±10.66). The number of 25(OH) Vitamin D deficient is higher in Covid-19 positive cases when compared to Covid-19 negative controls -46(52.3%) and 30(34%) respectively with a high statistically significant value (p-0.015).COVID-19 positive cases have higher tendency to have suboptimal plasma 25(OH) Vitamin D level which may contribute to the high hospitalization risk in COVID-19 infection. This finding is important as it can identify population at risk, and contribute to interventions in reducing the risk of hospitalization associated with COVID-19 infection.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"188 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73371925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.18231/j.ijcbr.2023.014
M. Coulibaly, Adama Kondé, D. Traoré, O. Bah, Valentin Sagara, B. Maiga
Blood cardiometabolic impairments are associated to high blood pressure which is a pivot cardiovascular risk factor. The objective of this study was to assess cardiometabolic risk factors rates in subjects with high blood pressure in the steady state. A total of 292subjects, 107 cases and 185 controls were enrolled in cross-sectional study. Clinical and biological data were assessed during visits and after overnight fasting. Data were analyzed on R. A p-value < 0.05 was considered for statistical significance.Univariate analysis showed thatage > 50 years, visceral obesity, metabolic syndrome and hs- CRP ≥ 3 mg/L were significant predictors of high blood pressure: OR = 2.1, 95% CI [1.3-3.5], p = 0.003; OR = 1.6, 95% CI [1.0-2.6], p = 0.05; OR = 3.3; 95% CI [2.0-5.4], p < 0.001; OR = 16.8; 95% CI [9.4-31.4], p < 0.001, respectively. Multivariate analysis showed a positive association between obesity, metabolic syndrome, hs-CRP and high blood pressure: aOR = 2.29; 95% CI [1.14-4.69], p = 0.02; aOR = 3.47; 95% CI [1.64-7.61], p = 0.001; aOR = 18.10; CI, 2.5% to 95% [9.40-36.99], p < 0.001, respectively. In contrast, female sex was negatively associated with high blood pressure aOR = 0.31; CI, 95% CI [0.13-0.72], p < 0.008. Prevention policiesshould take into account blood cardiometabolic level for subjects with high blood pressure even though in the steady sate.
血液心血管代谢障碍与高血压有关，而高血压是心血管疾病的主要危险因素。本研究的目的是评估处于稳定状态的高血压患者的心脏代谢危险因素率。横断面研究共纳入292名受试者，107例病例和185例对照。在访问期间和禁食过夜后评估临床和生物学数据。数据以r进行分析，以p < 0.05为差异有统计学意义。单因素分析显示，年龄0 ~ 50岁、内脏肥胖、代谢综合征和hs- CRP≥3mg /L是高血压的显著预测因素:OR = 2.1, 95% CI [1.3 ~ 3.5]， p = 0.003;OR = 1.6, 95% CI [1.0 ~ 2.6]， p = 0.05;Or = 3.3;95% CI [2.0 ~ 5.4]， p < 0.001;或= 16.8;95% CI [9.4 ~ 31.4]， p < 0.001。多因素分析显示肥胖、代谢综合征、hs-CRP与高血压呈正相关:aOR = 2.29;95% CI [1.14-4.69]， p = 0.02;aOR = 3.47;95% CI [1.64-7.61]， p = 0.001;aOR = 18.10;CI为2.5% ~ 95% [9.40 ~ 36.99]，p < 0.001。相反，女性与高血压呈负相关(or = 0.31);CI, 95% CI [0.13-0.72]， p < 0.008。预防政策应考虑到高血压患者的血液心脏代谢水平，即使处于稳定的安全状态。
{"title":"Effects of cardiometabolic risk factors on blood pressure in outpatients at Sominé DOLO hospital, Mopti, Mali","authors":"M. Coulibaly, Adama Kondé, D. Traoré, O. Bah, Valentin Sagara, B. Maiga","doi":"10.18231/j.ijcbr.2023.014","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.014","url":null,"abstract":"Blood cardiometabolic impairments are associated to high blood pressure which is a pivot cardiovascular risk factor. The objective of this study was to assess cardiometabolic risk factors rates in subjects with high blood pressure in the steady state. A total of 292subjects, 107 cases and 185 controls were enrolled in cross-sectional study. Clinical and biological data were assessed during visits and after overnight fasting. Data were analyzed on R. A p-value < 0.05 was considered for statistical significance.Univariate analysis showed thatage > 50 years, visceral obesity, metabolic syndrome and hs- CRP ≥ 3 mg/L were significant predictors of high blood pressure: OR = 2.1, 95% CI [1.3-3.5], p = 0.003; OR = 1.6, 95% CI [1.0-2.6], p = 0.05; OR = 3.3; 95% CI [2.0-5.4], p < 0.001; OR = 16.8; 95% CI [9.4-31.4], p < 0.001, respectively. Multivariate analysis showed a positive association between obesity, metabolic syndrome, hs-CRP and high blood pressure: aOR = 2.29; 95% CI [1.14-4.69], p = 0.02; aOR = 3.47; 95% CI [1.64-7.61], p = 0.001; aOR = 18.10; CI, 2.5% to 95% [9.40-36.99], p < 0.001, respectively. In contrast, female sex was negatively associated with high blood pressure aOR = 0.31; CI, 95% CI [0.13-0.72], p < 0.008. Prevention policiesshould take into account blood cardiometabolic level for subjects with high blood pressure even though in the steady sate.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87887140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.18231/j.ijcbr.2023.013
Nibedita Sarma, Subhosmito Chakraborty
Quality assurance is need of the hour in a laboratory. Clinician’s decision regarding treatment modality is based on the laboratory results on most occasions. So to ensure accuracy of results reported periodic evaluation as per standard criteria and audit is necessary. Six sigma is one such tool to apply in day to day practice for monitoring and enhancing performance of a laboratory. A new machine is a new challenge for a laboratory from validation to reporting of quality result with assurance for which six sigma is invariably a necessary method. So this study was taken up to check for month wise status of six sigma and performance of clinical chemistry analyser for 20 different analytes.: It was a retrospective study and data required for the study were collected from March 2020 to November 2020 in clinical biochemistry laboratory of Tata Medical Centre Kolkata, West Bengal. Test parameters were analysed on Vitros 7600 automated analyser. Data collected were IQC-coefficient of variation percent (CV %) and proficiency test -Bias%. Six sigma score were analysed monthly using standard formula applicable. We obtained an excellent performance (> 6 sigma) for test parameters CK, Urea, Creatinine, Uric acid, albumin, Calcium, Phosphorus, Magnesium, AST, ALTV, ALKP, GGT, Lipase, LDH in both level of IQC and for sodium and amylase in level 2 IQC. However we noticed poor performance (<3 sigma) for test parameters Glucose, potassium, chloride, TP in both level IQC and for sodium and amylase in level 2 IQC.QGI score analysed to find out root cause and corrective action. Monitoring IQC and EQAS with six sigma method helps evaluation and improvement of performance of a laboratory even with a new machine. It supports root cause analysis and necessary corrective and preventive action.
{"title":"Effectiveness of six sigma score analysis of clinical biochemistry parameters in a newly installed automated analyser– Retrospective analysis in a tertiary cancer care centre","authors":"Nibedita Sarma, Subhosmito Chakraborty","doi":"10.18231/j.ijcbr.2023.013","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.013","url":null,"abstract":"Quality assurance is need of the hour in a laboratory. Clinician’s decision regarding treatment modality is based on the laboratory results on most occasions. So to ensure accuracy of results reported periodic evaluation as per standard criteria and audit is necessary. Six sigma is one such tool to apply in day to day practice for monitoring and enhancing performance of a laboratory. A new machine is a new challenge for a laboratory from validation to reporting of quality result with assurance for which six sigma is invariably a necessary method. So this study was taken up to check for month wise status of six sigma and performance of clinical chemistry analyser for 20 different analytes.: It was a retrospective study and data required for the study were collected from March 2020 to November 2020 in clinical biochemistry laboratory of Tata Medical Centre Kolkata, West Bengal. Test parameters were analysed on Vitros 7600 automated analyser. Data collected were IQC-coefficient of variation percent (CV %) and proficiency test -Bias%. Six sigma score were analysed monthly using standard formula applicable. We obtained an excellent performance (> 6 sigma) for test parameters CK, Urea, Creatinine, Uric acid, albumin, Calcium, Phosphorus, Magnesium, AST, ALTV, ALKP, GGT, Lipase, LDH in both level of IQC and for sodium and amylase in level 2 IQC. However we noticed poor performance (<3 sigma) for test parameters Glucose, potassium, chloride, TP in both level IQC and for sodium and amylase in level 2 IQC.QGI score analysed to find out root cause and corrective action. Monitoring IQC and EQAS with six sigma method helps evaluation and improvement of performance of a laboratory even with a new machine. It supports root cause analysis and necessary corrective and preventive action.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85461804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.18231/j.ijcbr.2023.010
C-peptide levels can initially be normal or increased in type 2 diabetes mellitus (T2DM), which is connected to insulin resistance; however, as the condition progresses, these levels may fall. According to studies, kidney failure has diabetes as its primary cause, accounting for 44% of all new cases. In addition, it's critical to strictly regulate blood sugar levels and lower protein intake. Along with insulin, the pancreas produces a molecule called C-peptide. The hormone insulin regulates the body's glucose levels. A total of 300 subjects divided into 150 CKD progression diabetic nephropathy and 150 non-progressions diabetic nephropathy based on clinical parameter assessment. All patients had their body weight and body mass index (BMI) assessed. Fasting serum C peptide, HbA1C, serum creatinine, blood urea nitrogen, urine albumin, and creatinine were among the laboratory tests performed. Using the MDRD algorithm, creatinine clearance was determined from serum creatinine value. The parameters were statistically compared with respective subjects. The small case-control study found a no relationship between serum C-peptide levels and both microalbuminuria and HbA1C. Risk of microalbuminuria may be higher in patients with low serum C peptide levels. In patients with T2DM, replacing C-peptide and administering insulin may be useful. The possibility that C-peptide may play a part in the prevention and treatment of diabetic nephropathy will need to be investigated in studies including longer-term C-peptide administration.
{"title":"Role of circulating C-peptide in the Indian population with Type 2 diabetics with nephropathy: A triangular relationship between C-peptide, HbA1C and microalbuminuria","authors":"","doi":"10.18231/j.ijcbr.2023.010","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.010","url":null,"abstract":"C-peptide levels can initially be normal or increased in type 2 diabetes mellitus (T2DM), which is connected to insulin resistance; however, as the condition progresses, these levels may fall. According to studies, kidney failure has diabetes as its primary cause, accounting for 44% of all new cases. In addition, it's critical to strictly regulate blood sugar levels and lower protein intake. Along with insulin, the pancreas produces a molecule called C-peptide. The hormone insulin regulates the body's glucose levels. A total of 300 subjects divided into 150 CKD progression diabetic nephropathy and 150 non-progressions diabetic nephropathy based on clinical parameter assessment. All patients had their body weight and body mass index (BMI) assessed. Fasting serum C peptide, HbA1C, serum creatinine, blood urea nitrogen, urine albumin, and creatinine were among the laboratory tests performed. Using the MDRD algorithm, creatinine clearance was determined from serum creatinine value. The parameters were statistically compared with respective subjects. The small case-control study found a no relationship between serum C-peptide levels and both microalbuminuria and HbA1C. Risk of microalbuminuria may be higher in patients with low serum C peptide levels. In patients with T2DM, replacing C-peptide and administering insulin may be useful. The possibility that C-peptide may play a part in the prevention and treatment of diabetic nephropathy will need to be investigated in studies including longer-term C-peptide administration.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87064157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.18231/j.ijcbr.2023.009
Vaddiparti Aruna, N. Chowdeswari, Tummalagunta Nrusimha Murthy
Obesity, Metabolic syndrome and dyslipidaemia are gaining prevalence as noncommunicable causes of health hazards like CVD, IHD. National surveys under the leadership of ICMR were in progress to detect causes of high fat content in the body and means to detect the same early are underway. We made an attempt to know the importance of body fat percentage in Type 2 Diabetes Mellitus as marker of dyslipidaemia. : To study relationship of BMI, body fat percent (BFP) and lipid profile in type 2 Diabetes Mellitus.: 35 Type2DM (normotensive) subjects in the age group of 30-55yrs in both sexes and 35 age & sex matched healthy controls were selected for the study after obtaining informed consent. Anthropometric data was recorded. Their fasting venous blood samples were analysed for sugar & Lipid profile ERBA Chem 5 semiautoanalyser. BMI and BFP were calculated.: BFP was well above normal levels in both cases and controls (38.03±12.13 &44.9±7.66 respectively) for the age group of 30-55yrs normal limits being 19,3% -26.3% according to Jackson & Pollard. The mean BMI of cases was 33.22±8.59 compared to 37.07±4.1 of controls, indicating both groups were class II obese individuals. BMI & BFP showed statistically significant correlation between cases and controls by Student’s ‘t’ test (p <0.009 & <0.003 respectively) and also within the groups (P <0.00001) by way of ANOVA. Analysis was done to detect gender difference. In males and females there was strongly positive correlation of BMI as detected by Pearson’s Correlation coefficient (r = 0.914 & 0.976). TC, TG & HDL showed weak positive correlation with BMI & BFP in diabetic subjects, whereas LDL correlated negatively with BMI & BFP.: We could not conclude superiority of BFP over BMI in assessing dyslipidaemia. Nevertheless, the study can be done using other methods of evaluation of BFP in T2DM like ultrasound, computerised tomography.
{"title":"Body fat percentage utility over lipid profile as marker of dyslipidemia in type 2 diabetes mellitus","authors":"Vaddiparti Aruna, N. Chowdeswari, Tummalagunta Nrusimha Murthy","doi":"10.18231/j.ijcbr.2023.009","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.009","url":null,"abstract":"Obesity, Metabolic syndrome and dyslipidaemia are gaining prevalence as noncommunicable causes of health hazards like CVD, IHD. National surveys under the leadership of ICMR were in progress to detect causes of high fat content in the body and means to detect the same early are underway. We made an attempt to know the importance of body fat percentage in Type 2 Diabetes Mellitus as marker of dyslipidaemia. : To study relationship of BMI, body fat percent (BFP) and lipid profile in type 2 Diabetes Mellitus.: 35 Type2DM (normotensive) subjects in the age group of 30-55yrs in both sexes and 35 age & sex matched healthy controls were selected for the study after obtaining informed consent. Anthropometric data was recorded. Their fasting venous blood samples were analysed for sugar & Lipid profile ERBA Chem 5 semiautoanalyser. BMI and BFP were calculated.: BFP was well above normal levels in both cases and controls (38.03±12.13 &44.9±7.66 respectively) for the age group of 30-55yrs normal limits being 19,3% -26.3% according to Jackson & Pollard. The mean BMI of cases was 33.22±8.59 compared to 37.07±4.1 of controls, indicating both groups were class II obese individuals. BMI & BFP showed statistically significant correlation between cases and controls by Student’s ‘t’ test (p <0.009 & <0.003 respectively) and also within the groups (P <0.00001) by way of ANOVA. Analysis was done to detect gender difference. In males and females there was strongly positive correlation of BMI as detected by Pearson’s Correlation coefficient (r = 0.914 & 0.976). TC, TG & HDL showed weak positive correlation with BMI & BFP in diabetic subjects, whereas LDL correlated negatively with BMI & BFP.: We could not conclude superiority of BFP over BMI in assessing dyslipidaemia. Nevertheless, the study can be done using other methods of evaluation of BFP in T2DM like ultrasound, computerised tomography.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"263 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75045782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.18231/j.ijcbr.2023.011
Akhilesh Kumar Singh, M. Kumar, Manvendra Singh
The major objective of this research was to study the effect of different levels of kiwi () fruit pulp on physio-chemical properties of yogurt. During the study goat (capra hircus) milk yogurt incorporated with kiwi fruit pulp was analyzed for physico- chemical properties like fat, protein, moisture, total solids, ash, pH, synersis, acidity and colour properties etc. Yogurt was prepared by using different levels of kiwi fruit pulp. The data were statistically analysed by using Central composite rotatable design. The yogurt incorporated by kiwi fruit pulp different levels (0.20 to 2.50) showed overall acceptability sensory score. The chemical composition of yogurt prepared by addition of (0.20 to 2.50) kiwi fruit pulp having fat 0.20%, protein 0.17%, moisture 83.07%, total solids 16.70%, ash 0.15%, pH 3.26, Titrable acidity 1.43% and carbohydrate 14.67 percent.
{"title":"A study on physic-chemical properties of goat milk yoghurt incorporated with kiwi fruit () pulp","authors":"Akhilesh Kumar Singh, M. Kumar, Manvendra Singh","doi":"10.18231/j.ijcbr.2023.011","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.011","url":null,"abstract":"The major objective of this research was to study the effect of different levels of kiwi () fruit pulp on physio-chemical properties of yogurt. During the study goat (capra hircus) milk yogurt incorporated with kiwi fruit pulp was analyzed for physico- chemical properties like fat, protein, moisture, total solids, ash, pH, synersis, acidity and colour properties etc. Yogurt was prepared by using different levels of kiwi fruit pulp. The data were statistically analysed by using Central composite rotatable design. The yogurt incorporated by kiwi fruit pulp different levels (0.20 to 2.50) showed overall acceptability sensory score. The chemical composition of yogurt prepared by addition of (0.20 to 2.50) kiwi fruit pulp having fat 0.20%, protein 0.17%, moisture 83.07%, total solids 16.70%, ash 0.15%, pH 3.26, Titrable acidity 1.43% and carbohydrate 14.67 percent.","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"137 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89212480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-15DOI: 10.18231/j.ijcbr.2023.003
Sudhir Kumar Khuntia, Reena Thakur
A dangerous fungal infection that has recently emerged as a hazard for COVID-19 survivors, mucormycosis was first identified in Bangladesh on May 8, 2021, and is now commonly referred to as "black fungus" in the COVID-19 period. During the COVID-19 epidemic, black fungi are eighty times more common in India. The COVID-19 black fungi has spread to numerous nations, including Bangladesh, Pakistan, Russia, Iran, and Brazil. Being physically close to India, Bangladesh is concerned about the worrisome rise in COVID-19-related black fungus infections in India, which have a high fatality rate of about 50%. Pre-experimental design with one-group pre-test and post-test study method was used to examine staff nurses' understanding of black fungus avoidance and control; the participants were chosen through handy sampling. The data collected was analyzed using descriptive and inferential statistics in terms of frequency, mean, standard deviation, and associated by chi square test. Among 60 staff nurse in pre-test, 29 (48.33%) have poor knowledge, 27 (45%) have average knowledge, 4 (6.67%) having good, and in post-test 44 (33.33%) having good knowledge, 16 (26.67%) having average knowledge.Under Pre-test the Mean of the Scores was 8.3 (55.33%) under the Post- test the Mean was observed as 12.2 (81.33%).
{"title":"The effect of video assisted teaching programme on knowledge regarding prevention and management of black fungus among staff nurse","authors":"Sudhir Kumar Khuntia, Reena Thakur","doi":"10.18231/j.ijcbr.2023.003","DOIUrl":"https://doi.org/10.18231/j.ijcbr.2023.003","url":null,"abstract":"A dangerous fungal infection that has recently emerged as a hazard for COVID-19 survivors, mucormycosis was first identified in Bangladesh on May 8, 2021, and is now commonly referred to as \"black fungus\" in the COVID-19 period. During the COVID-19 epidemic, black fungi are eighty times more common in India. The COVID-19 black fungi has spread to numerous nations, including Bangladesh, Pakistan, Russia, Iran, and Brazil. Being physically close to India, Bangladesh is concerned about the worrisome rise in COVID-19-related black fungus infections in India, which have a high fatality rate of about 50%. Pre-experimental design with one-group pre-test and post-test study method was used to examine staff nurses' understanding of black fungus avoidance and control; the participants were chosen through handy sampling. The data collected was analyzed using descriptive and inferential statistics in terms of frequency, mean, standard deviation, and associated by chi square test. Among 60 staff nurse in pre-test, 29 (48.33%) have poor knowledge, 27 (45%) have average knowledge, 4 (6.67%) having good, and in post-test 44 (33.33%) having good knowledge, 16 (26.67%) having average knowledge.Under Pre-test the Mean of the Scores was 8.3 (55.33%) under the Post- test the Mean was observed as 12.2 (81.33%).","PeriodicalId":13899,"journal":{"name":"International Journal of Clinical Biochemistry and Research","volume":"100 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76082106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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