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Key locations of oxidative damage in human hair keratins after heat and ultraviolet light exposure 高温和紫外线照射后人体头发角蛋白氧化损伤的关键部位。
IF 2.5 4区 医学 Q2 DERMATOLOGY Pub Date : 2025-09-10 DOI: 10.1111/ics.13081
Jeffrey E. Plowman, Anita J. Grosvenor, Santanu Deb-Choudhury, Fraser Bell, Julie Roberts, Stefan Clerens

Objective

This study investigated the locations of amino acid modifications within two major human hair keratins (Type I K31 and Type II K85) with probable implications for protein and hair structural component integrity. The particular focus was on cysteine modifications that disrupt intra-protein and inter-protein disulphide bonds.

Methods

Human hair was exposed to accelerated, sequential heat or UV treatments, simulating effects resulting from the use of heated styling tools and environmental exposure over a time frame approximating one year. The proteins were extracted and detected using LC–MS/MS and identified by matching to protein databases. The sites of disulphide-disrupting amino acid residue modifications were mapped to known structural domains of the keratins.

Results

Disulphide bond-disrupting cysteine modifications (formation of cysteic acid and dehydroalanine) arose in K31 and K85 after treatments with UV and with heat. These modifications were mapped to both the relatively amorphous (head) regions of the keratins, which bind with keratin-associated proteins, and the more structured coil regions, which bind to other keratins.

Conclusion

These data provide confirmation that heat- and UV-induced disulphide bond disruption resulted in post-translational modifications that are crucial for secondary structural interactions, the loss of which has the potential to affect the loss of structural integrity observed in human hair after the use of repeated styling processes or prolonged weathering from sunlight exposure.

目的:本研究研究了两种主要的人类头发角蛋白(I型K31和II型K85)中氨基酸修饰的位置,这可能对蛋白质和头发结构成分的完整性有影响。特别关注的是半胱氨酸修饰,破坏蛋白质内和蛋白质间的二硫键。方法:将人的头发暴露在加速的、连续的加热或紫外线处理中,模拟使用加热造型工具和环境暴露在大约一年的时间内所产生的效果。采用LC-MS/MS提取和检测蛋白质,并与蛋白质数据库进行比对鉴定。二硫化物破坏氨基酸残基修饰的位点被映射到角蛋白的已知结构域。结果:K31和K85在紫外线和高温处理后出现了破坏二硫键的半胱氨酸修饰(半胱酸和脱氢丙氨酸的形成)。这些修饰被映射到角蛋白的相对无定形(头部)区域,它与角蛋白相关蛋白结合,以及更结构化的线圈区域,它与其他角蛋白结合。结论:这些数据证实,热和紫外线诱导的二硫化物键破坏导致翻译后修饰,这对二级结构相互作用至关重要,二级结构相互作用的丧失有可能影响在使用重复定型过程或长时间暴露于阳光下后观察到的人类头发结构完整性的丧失。
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引用次数: 0
Correction to International Journal of Cosmetic Science: Volume 47, Issue 4 修正国际化妆品科学杂志:第47卷,第4期
IF 2.5 4区 医学 Q2 DERMATOLOGY Pub Date : 2025-09-08 DOI: 10.1111/ics.70022

The image caption on the cover of Volume 47, Issue 4 was incorrect.

It should have been:

Keratin liposomes for the recovery of photo-damaged hair

We apologize for this error.

第47卷第4期封面上的图片说明不正确。它应该是:修复光损伤头发的角蛋白脂质体我们为这个错误道歉。
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引用次数: 0
Bioretinoids from microalgae: Boosting retinol performance and tolerability 来自微藻的类生物维生素a:提高视黄醇性能和耐受性。
IF 2.5 4区 医学 Q2 DERMATOLOGY Pub Date : 2025-09-04 DOI: 10.1111/ics.70027
Silvia Sánchez-Díez, Alexandre Lapeyre, Noemí García-Delgado, Jordi Ayats

Objective

Skin ageing, hyperpigmentation, and texture are common dermatological concerns. Retinoids, including retinol, are widely used for their efficacy in treating these conditions. However, their side effects, such as irritation, often limit their use. Bakuchiol, a plant-derived retinoid alternative, has gained attention for its gentler profile, but its efficacy remains a subject of comparison with traditional retinoids. The aim of the present study was to evaluate the efficacy of a novel microalgae-derived bioretinoid (MBR) in comparison to retinol and bakuchiol in promoting skin regeneration, reducing ageing signs, and addressing hyperpigmentation.

Methods

The effects of MBR were evaluated in vitro for cell proliferation, melanin production, and extracellular matrix modulation. Clinically, MBR was applied to assess improvements in skin texture, firmness, elasticity, and pigmentation compared with reference treatments.

Results

In vitro, MBR significantly outperformed both retinol and bakuchiol in cell proliferation, melanin reduction, and ECM modulation. In vivo, MBR improved skin texture, firmness, and pigmentation, showing results comparable to retinol. MBR also synergistically enhanced retinol's effects, improving hydration and reducing irritation.

Conclusion

MBR is a promising alternative to traditional retinoids, offering enhanced efficacy in skin regeneration and pigmentation control with a more favourable safety profile, especially when combined with retinol.

目的:皮肤老化,色素沉着和纹理是常见的皮肤病学问题。类维生素a,包括视黄醇,因其治疗这些疾病的功效而被广泛使用。然而,它们的副作用,如刺激,往往限制了它们的使用。Bakuchiol是一种植物衍生的类维甲酸替代品,因其温和的特性而受到关注,但其功效仍然是与传统类维甲酸进行比较的主题。本研究的目的是评估一种新型微藻衍生的生物类维甲酸(MBR)在促进皮肤再生、减少衰老迹象和解决色素沉着方面的功效,与视黄醇和bakuchiol进行比较。方法:体外观察MBR对细胞增殖、黑色素生成和细胞外基质调节的影响。在临床上,与参考治疗相比,MBR应用于评估皮肤质地、紧致度、弹性和色素沉着的改善。结果:在体外,MBR在细胞增殖、黑色素减少和ECM调节方面明显优于视黄醇和bakuchiol。在体内,MBR改善了皮肤质地、紧致度和色素沉着,其结果与视黄醇相当。MBR还协同增强视黄醇的作用,改善水合作用,减少刺激。结论:MBR是一种很有前景的替代传统类维生素a的方法,在皮肤再生和色素沉着控制方面具有更强的功效,并且更有利的安全性,特别是与视黄醇联合使用时。
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引用次数: 0
Recovery effects of various skin lipid components and their structural derivatives on skin barrier damage 各种皮肤脂质成分及其结构衍生物对皮肤屏障损伤的恢复作用。
IF 2.5 4区 医学 Q2 DERMATOLOGY Pub Date : 2025-09-04 DOI: 10.1111/ics.70023
Sang Gwon Seo, Tae Geun Kwon, Ji Won Ha, Jooyoung Oh, Moon Joo Kim, Sohyun In

Objective

Skin lipids, such as ceramides (CERs), play an important role in maintaining the skin barrier. Although the benefits of CERs are well known, few studies have investigated the effects of other skin lipid components on the skin barrier. Therefore, this study compared the efficacy of several skin lipid components and their structural derivatives, including CERs, on the skin barrier.

Methods

The water-holding capacity and occlusion factor were determined to evaluate the moisture barrier and sealing effects, respectively, of creams containing various skin lipid components. A human study using sodium dodecyl sulfate (SDS) to induce skin damage was also conducted to explore skin barrier recovery. Epidermal thickness and density were measured using an ultrasonic instrument.

Results

The cream containing behenic acid (BA) had the best water-holding capacity, and the creams with CER and BA significantly increased the occlusion factor. The BA-containing cream had a cubic structural pattern in the small-angle X-ray scattering analysis, which may explain the high occlusion factor. In SDS-damaged skin, transepidermal water loss recovered significantly faster in skin areas treated with cream containing BA and CER compared with non-treated skin.

Conclusion

This study compares the skin barrier recovery effects of skin lipid components, demonstrating that BA elicits a high skin barrier recovery effect similar to that of CER, which has known efficacy. BA also had an excellent sealing effect through lipid packaging, whereas CER had a combined sealing effect and increased the epidermal thickness and density. These results provide foundational evidence for formulating products that help restore the skin barrier by supplying lipids to the skin surface.

目的:神经酰胺(CERs)等皮肤脂质在维持皮肤屏障中起重要作用。虽然cer的益处是众所周知的,但很少有研究调查其他皮肤脂质成分对皮肤屏障的影响。因此,本研究比较了几种皮肤脂质成分及其结构衍生物(包括cer)对皮肤屏障的作用。方法:测定不同脂质成分面霜的保水性和封闭系数,分别评价其保湿效果和封闭效果。使用十二烷基硫酸钠(SDS)诱导皮肤损伤的人体研究也进行了探讨皮肤屏障恢复。用超声仪测定表皮厚度和密度。结果:含褪黑酸(BA)的乳膏保水能力最好,含CER和BA的乳膏明显增加了闭塞因子。在小角度x射线散射分析中,含ba乳膏呈立方结构,这可能是高遮挡因子的原因。在sds损伤的皮肤中,与未处理的皮肤相比,使用含有BA和CER的乳膏处理的皮肤区域经皮失水恢复明显更快。结论:本研究比较了皮肤脂质成分的皮肤屏障恢复效果,表明BA具有与CER相似的高皮肤屏障恢复效果,具有已知的功效。BA通过脂质包装也具有良好的密封效果,而CER具有复合密封效果,增加了表皮厚度和密度。这些结果为配方产品提供了基础证据,这些产品通过向皮肤表面提供脂质来帮助恢复皮肤屏障。
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引用次数: 0
Influence of anionic surfactants on coacervation with cationic guar gum for hair care applications 阴离子表面活性剂对护发用阳离子瓜尔胶凝聚的影响。
IF 2.5 4区 医学 Q2 DERMATOLOGY Pub Date : 2025-09-04 DOI: 10.1111/ics.70025
Gael Alexander López-Sucilla, Sergio Alberto Bernal-Chávez

Objective

To assess the impact of anionic surfactants on the formation of coacervates with cationic guar gum and their subsequent effects on hair care.

Methods

Coacervates were prepared using ionic precipitation techniques involving four anionic surfactants: sodium lauryl sulfate (SLS), sodium lauroyl sarcosinate (SNL), sodium lauryl sulfoacetate (SLSA), sodium cocoyl isethionate (SCI) and cationic guar gum. Viscosity, spreadability and stickiness sensory analysis were conducted with a panel of volunteers. The coacervates were freeze-dried, and their physicochemical properties were analysed using Raman and Fourier transform infrared spectroscopy, scanning electron microscopy and thermogravimetric analysis.

Results

Coacervate formation and stability were dependent on the CGG-to-surfactant ratio, with 0.5% CGG/0.5% surfactant producing the most stable, uniform structures. Spectroscopic analyses confirmed stronger electrostatic and hydrogen bonding interactions at higher CGG concentrations, facilitating greater polymer-surfactant integration. Thermal analysis showed that balanced CGG/surfactant ratios enhanced coacervate stability, while excess surfactant led to lower thermal resistance and weaker deposition. SEM images revealed superior adhesion and homogeneous coverage on damaged hair fibres at 0.5% CGG/0.5% surfactant, suggesting better conditioning effects compared to lower polymer content.

Conclusion

The findings emphasize that coacervate stability, deposition efficiency and adhesion to hair fibres are optimized when CGG and surfactants are present in balanced ratios. The 0.5% CGG/0.5% surfactant formulation consistently exhibited superior stability, coverage and interaction with damaged hair, reinforcing its potential as an effective conditioning and protective system. These insights provide a foundation for improving coacervate-based hair care formulations, ensuring enhanced performance and long-term benefits.

目的:探讨阴离子表面活性剂对瓜尔胶与阳离子瓜尔胶凝聚形成的影响及其对护发的影响。方法:采用离子沉淀法,采用四种阴离子表面活性剂:月桂基硫酸钠(SLS)、月桂基肌酸钠(SNL)、月桂基硫乙酸钠(SLSA)、椰油基异硫酸钠(SCI)和阳离子瓜尔胶制备凝聚体。对一组志愿者进行了粘度、铺展性和粘性感官分析。用拉曼光谱、傅里叶变换红外光谱、扫描电镜和热重分析等方法分析了凝聚体的理化性质。结果:聚簇的形成和稳定性取决于CGG与表面活性剂的比例,0.5% CGG/0.5%表面活性剂的聚簇结构最稳定、均匀。光谱分析证实,在较高的CGG浓度下,静电和氢键相互作用更强,促进了聚合物与表面活性剂的结合。热分析表明,平衡的CGG/表面活性剂比例增强了凝聚稳定性,而过量的表面活性剂导致热阻降低,沉积减弱。SEM图像显示,在0.5% CGG/0.5%表面活性剂的情况下,损伤的头发纤维具有较好的粘附性和均匀覆盖,表明与低聚合物含量相比,调节效果更好。结论:CGG和表面活性剂以平衡比例存在时,凝聚稳定性、沉积效率和对毛纤维的粘附性均得到优化。0.5% CGG/0.5%表面活性剂配方始终表现出优异的稳定性,覆盖范围和与受损头发的相互作用,增强了其作为有效调理和保护系统的潜力。这些见解为改进基于凝聚体的护发配方提供了基础,确保提高性能和长期效益。
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引用次数: 0
Strengthening benefits of panthenol for hair: Mechanistic evidence from advanced spectroscopic techniques 泛醇对头发的强化益处:来自先进光谱技术的机理证据。
IF 2.5 4区 医学 Q2 DERMATOLOGY Pub Date : 2025-09-04 DOI: 10.1111/ics.70024
Jennifer M. Marsh, Scott Cron, Tian Chen, Allison Talley, Shane Whitaker, Haibo Jiang, Kai Xue

Objective

Small molecules, like panthenol have been shown to penetrate hair. However, their interactions with the protein structures of hair have not been well studied. In this study, the penetration of panthenol into protein structures of hair was confirmed, and NMR methods were utilized to understand the protein–panthenol interaction. This interaction is likely the source of the tensile strength benefits measured with panthenol for hair.

Methods

Cross-sections of hair treated with deuterium-labelled panthenol were studied using NanoSIMS to visualize the location of panthenol within hair. Solid-state NMR (both 1H–13C and 1H–2H) was employed to measure panthenol interactions inside hair using deuterium-labelled panthenol. Stress/strain curves—where stress is defined as average force per unit area—were measured for hair treated with and without panthenol to generate tensile strength data.

Results

Penetration of panthenol into hair was demonstrated, with images indicating specific penetration into cortical protein regions. NMR results from various experiments provided evidence of direct interaction between the N–H of panthenol and protein aromatic side chains. A ‘shared’ proton was identified between panthenol and hair proteins, indicating spatial proximity and interaction. Break stress and elastic modulus of hair treated with panthenol were higher than those of control hair, suggesting that bonds—likely hydrogen bonds—were formed between panthenol and hair.

Conclusion

Panthenol can deliver tensile strength benefits to hair. This benefit is proposed to result from hydrogen bonds formed between panthenol and hair proteins.

目的:像泛醇这样的小分子已经被证明可以穿透头发。然而,它们与头发蛋白质结构的相互作用还没有得到很好的研究。在本研究中,证实了泛醇对头发蛋白质结构的渗透,并利用NMR方法了解了蛋白质-泛醇的相互作用。这种相互作用很可能是泛醇对头发抗拉强度有益的来源。方法:利用NanoSIMS对经氘标记的泛醇处理过的头发的横截面进行研究,以显示泛醇在头发中的位置。采用固体核磁共振(1H-13C和1H-2H)测量头发内氘标记的泛醇相互作用。应力/应变曲线——其中应力被定义为单位面积的平均力——测量了用和不用泛醇处理的头发,以产生抗拉强度数据。结果:证实了泛醇对头发的渗透,图像显示了对皮层蛋白质区域的特异性渗透。各种实验的核磁共振结果提供了泛醇的N-H与蛋白质芳香侧链之间直接相互作用的证据。在泛醇和毛发蛋白之间发现了一个“共享”质子,表明空间接近和相互作用。用泛醇处理过的头发的断裂应力和弹性模量比对照组的头发要高,这表明泛醇和头发之间形成了键——很可能是氢键。结论:泛醇可以提高头发的抗拉强度。这种益处被认为是由于泛醇和头发蛋白之间形成的氢键。
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引用次数: 0
Sunscreen sensory attribute prediction via textural, rheological and tribological tests 通过纹理、流变学和摩擦学测试预测防晒霜的感官属性。
IF 2.5 4区 医学 Q2 DERMATOLOGY Pub Date : 2025-09-04 DOI: 10.1111/ics.70021
Günel Nabiyeva, Baran Teoman, Andrei Potanin, Gennady Y. Gor

This study aims to find the quick ways to predict sensorial attributes of sunscreens using instrumental measurements. We specifically focus on the attributes most relevant to consumer ‘liking’ and the easiest measurements to implement in industrial labs for product development. Of the latter, we consider three types of measurements: emulative ‘textural’ tests typically performed with a texture analyser as well as rheological and tribological measurements. Textural tests are generally preferred but may be paralleled by rheological measurements provided they are implementable on simple viscometers used in industry (albeit with additional data processing). We found that both textural and simple rheological/viscometrical tests work well for predicting sensorial attributes about flow before (thickness) and during (spreadability) application of the products. Tribology provides useful additional information for the latter but is primarily useful for after-feel or residue attributes (absorption and skin stickiness). These findings show that simple lab tests relying on these techniques can predict human sensory attributes, thus efficiently guide product developers while reducing the need for costly and time-consuming panel studies.

本研究旨在通过仪器测量,找到快速预测防晒霜感官属性的方法。我们特别关注与消费者“喜欢”最相关的属性,以及最容易在工业实验室实施的产品开发测量。对于后者,我们考虑了三种类型的测量:模拟“纹理”测试,通常使用纹理分析仪进行,以及流变学和摩擦学测量。纹理测试通常是首选,但也可以与流变测量并行,前提是它们可以在工业中使用的简单粘度计上实现(尽管需要额外的数据处理)。我们发现,质地和简单的流变/粘度测试都可以很好地预测产品应用前(厚度)和应用期间(涂抹性)的流动感官属性。摩擦学为后者提供了有用的附加信息,但主要用于后感或残留属性(吸收和皮肤粘性)。这些发现表明,依靠这些技术的简单实验室测试可以预测人类的感官属性,从而有效地指导产品开发人员,同时减少对昂贵和耗时的面板研究的需要。
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引用次数: 0
Test design and results of a method performance characterization study for SPF and UVA-PF testing SPF和UVA-PF测试方法性能表征研究的试验设计和结果
IF 2.5 4区 医学 Q2 DERMATOLOGY Pub Date : 2025-09-01 DOI: 10.1111/ics.70019
Bertrand Colson, Jürgen Vollhardt, Curtis Cole, Ludger Kolbe, Paul Matts, Benoit Muller, Luciano Nogueira, Sergio Oliveira, Marc Pissavini, Florence Pouradier, Gerald Renner, Mathias Rohr, Eduardo Ruvolo, Caroline Tricaud, ALT-SPF Consortium, Steffen Uhlig

Objective

This paper presents the experimental design, the statistical model, performance criteria and results of a method performance study conducted in order to characterize the performance of alternative methods for SPF and UVA-PF testing. The performance of alternative methods is characterized against the performance of the ISO 24444 and ISO 24443 methods.

Methods

A factorial experimental design was implemented. For a given method, such a design allows good statistical reliability of precision estimates with as few as four participating laboratories. To cover the wide range of sunscreen products on the market, 32 sunscreens were tested. These 32 products were classified into eight product groups. The product groups differ in terms of SPF level, application viscosity and type of UV filters and matrix. In co-operation with a WG7 ISO ad hoc group performance criteria for the alternative methods were developed for reproducibility precision, between-laboratory variation, bias and the variation of bias across products and across product groups.

Results

Performance characteristics for the reference method along with reproducibility and repeatability limits are provided. The results for the participating alternative methods will be presented in separate articles, following this paper.

Conclusions

The precision of ISO 24444 and ISO 24443 was characterized for each of the eight product groups. The main sources of variation were identified for each of the two reference methods. Reproducibility and repeatability limits were derived and are provided.

目的介绍SPF和UVA-PF两种测试方法的实验设计、统计模型、性能标准和测试结果。替代方法的性能与ISO 24444和ISO 24443方法的性能相比较。方法采用因子试验设计。对于一个给定的方法,这样的设计允许只有四个参与实验室的精度估计具有良好的统计可靠性。为了覆盖市场上各种各样的防晒产品,我们对32种防晒霜进行了测试。这32种产品被分为8个产品组。产品组在SPF级别,应用粘度和紫外线过滤器和基质的类型方面有所不同。与WG7 ISO特设小组合作,开发了可选方法的可重复性精度,实验室间差异,偏差以及产品和产品组之间偏差的变化。结果提供了参考方法的性能特征及重现性和重复性限制。参与替代方法的结果将在本文之后的单独文章中介绍。结论ISO 24444和ISO 24443对8个产品组的精密度进行了表征。确定了两种参考方法的主要变异源。推导并给出了再现性和重复性极限。
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引用次数: 0
Are there alternatives to the traditional in-vivo SPF test (ISO 24444)? Comparison and statistical analysis of 5 proposed methods 有替代传统体内SPF测试(ISO 24444)的方法吗?提出的5种方法的比较与统计分析
IF 2.5 4区 医学 Q2 DERMATOLOGY Pub Date : 2025-09-01 DOI: 10.1111/ics.70026
Marc Pissavini, John Chave, Majella Lane

Sun protection products play a critical role in defending consumers' skin against the harmful effects of ultraviolet radiation during sun overexposure. Regular use of these products is essential, but it is equally important to ensure that the sun protection factor (SPF) communicated to consumers is accurate and allows consistent comparison between products. This is particularly vital since the vast majority of consumers base their purchasing decisions on the SPF value displayed on the label.

The current international standard for SPF testing, ISO 24444:2019, relies on testing conducted with human volunteers. Until 2024, this was the sole accepted method for SPF evaluation. However, this approach is invasive, complex, time-consuming, costly, and – as a biological test – has a high variability. Over many years, the cosmetics industry has been working to develop suitable alternative methods that give comparable results to ISO 24444:2019.

Validating such new methods is done through ring tests that benchmark the results of the new test methods against the gold standard. This requires the availability of robust in vivo reference SPF data on a large number of sun protection products – a significant experimental undertaking that cannot be repeated for each proposed method.

Recognizing this need, a consortium of sunscreen testing users and developers launched the ALT-SPF initiative in 2018 to identify, characterize, and evaluate alternative sunscreen testing methods against the ISO 24444:2019 gold standard. It included SPF testing methods that were expected to be less invasive, more precise, and more cost-effective. As part of this effort, ALT-SPF conducted an extensive study using 32 sunscreen samples. These were tested with the current ISO 24444:2019 standard and five alternative methods, including in vitro, in silico, and non-invasive in vivo testing approaches.

Each alternative method was benchmarked against the gold standard, with results analysed through statistically robust models. This rigorous approach not only evaluated the alternative methods but also laid the foundation for ISO's landmark publication in December 2024 of two alternative SPF testing methods – marking the first time non-in vivo approaches have been formally recognized as viable options to ISO 24444.

The editors, Professor Majella Lane and Professor Marc Pissavini, are honoured to welcome Mr. John Chave, Director General of Cosmetics Europe, as the guest editor of this special edition of the International Journal of Cosmetic Science. His participation underscores the importance of collaborative efforts and expertise in advancing SPF testing methodologies.

This edition features seven groundbreaking publications authored by leading experts. These papers detail the protocols, findings, and conclusions of the ALT-SPF initiative. Their contributions have been instrumental in advancing the science of SPF testing and shaping a future where in

在过度暴露在阳光下时,防晒产品在保护消费者皮肤免受紫外线辐射的有害影响方面发挥着关键作用。定期使用这些产品是必不可少的,但同样重要的是要确保向消费者传达的防晒系数(SPF)是准确的,并允许产品之间的一致比较。这一点尤其重要,因为绝大多数消费者的购买决定都是基于标签上显示的SPF值。目前的国际SPF测试标准ISO 24444:2019依赖于对人类志愿者进行的测试。直到2024年,这是唯一被接受的SPF评估方法。然而,这种方法是侵入性的、复杂的、耗时的、昂贵的,而且作为一种生物学测试,具有很高的可变性。多年来,化妆品行业一直在努力开发合适的替代方法,以提供与ISO 24444:2019相当的结果。通过环形测试来验证这些新方法,将新测试方法的结果与金标准进行基准测试。这需要大量防晒产品的体内参考SPF数据的可用性,这是一项重要的实验工作,不能对每种提出的方法重复。认识到这一需求,一个由防晒霜测试用户和开发者组成的联盟于2018年发起了ALT-SPF倡议,以根据ISO 24444:2019黄金标准识别、表征和评估替代防晒霜测试方法。它包括SPF测试方法,这些方法被认为侵入性更小,更精确,更具成本效益。作为这项工作的一部分,ALT-SPF使用32种防晒霜样本进行了广泛的研究。这些测试采用现行的ISO 24444:2019标准和五种替代方法进行测试,包括体外、计算机和非侵入性体内测试方法。每种替代方法都以金标准为基准,并通过统计稳健模型对结果进行分析。这种严格的方法不仅评估了替代方法,而且为ISO于2024年12月发布的两种替代SPF测试方法奠定了基础,这标志着非体内方法首次被正式认可为ISO 24444的可行选择。编辑Majella Lane教授和Marc Pissavini教授荣幸地欢迎欧洲化妆品总干事John Chave先生担任本期《国际化妆品科学杂志》特刊的客座编辑。他的参与强调了合作努力和专业知识在推进SPF测试方法方面的重要性。这个版本有七个开创性的出版物由领先的专家撰写。这些论文详细介绍了ALT-SPF计划的方案、发现和结论。他们的贡献有助于推动SPF测试科学的发展,并塑造一个创新、可靠和合乎道德的方法占据中心地位的未来。我们希望这个特别版能为您提供有关SPF测试未来的深刻见解,并强调合作在推动科学进步中的重要性。我们祝你读得愉快。真诚,编辑器:
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引用次数: 0
Characterization of LED-based hybrid diffuse reflectance spectroscopy method for determination of SPF and UVA-PF in blinded multi-centre study (ALT-SPF) 基于led的混合漫反射光谱法测定SPF和UVA-PF的盲法多中心研究(ALT-SPF)
IF 2.5 4区 医学 Q2 DERMATOLOGY Pub Date : 2025-09-01 DOI: 10.1111/ics.70007
Carina Reble, Michael Bayer, Bertrand Colson, Tanja Emmler, Jean-Claude Hubaud, Matthias Seise, Eva Perroux-David, Georg Wiora, Daniela F. Zamudio Diaz, ALT-SPF Consortium, Martina C. Meinke, Stephan Bielfeldt

Objective

The consortium ALT-SPF performed an international round robin test to characterize non-invasive methods as alternatives to the erythema-based testing of sun protection factor (SPF) according to ISO 24444:2019.

Methods

Hybrid diffuse reflectance spectroscopy (HDRS) based on a multi-lambda LED light source uses in vivo reflectance spectra on skin to determine sunscreens in vivo absorbance spectra, which are fused with respective in vitro absorbance spectra measured as thin films transmission as described in ISO 24443:2019. As a part of the ALT-SPF consortium initiative, a blinded study on 64 samples was performed in four European laboratories. After further improvements of the method, a blinded re-evaluation based on 16 samples was performed. Five statistical acceptance criteria for new methods were assessed by an independent statistical institute to compare the obtained results to the reference methods for SPF and UVA-PF.

Results

The initial ALT-SPF study 1 showed that the bias criterion was acceptable, while the reproducibility and interlaboratory variability needed further improvement. The re-evaluation study 2 showed that the reproducibility and interlaboratory variability could be considerably improved. Using only n = 10 volunteers and a bias correction based on the initial ALT-SPF study 1 data, the SPF results of the re-evaluation study 2 were close to the acceptance criteria of the ALT-SPF study with criterion 1 (reproducibility) and only 11% over the limit defined by the performance of the gold standard. The UVA-PF results were within the acceptance limits for the acceptance criteria, except criterion 3 being in the ‘almost met’ range. The re-evaluation study indicates that the method has a comparable precision to the gold standard methods ISO 24444 and ISO 24443.

Conclusion

This study showed that the LED-HDRS method is capable of providing reasonable non-invasive SPF and UVA-PF results and that the performance shows close alignment to the reference method. Thus, it can be proposed as an alternative method to the current standards ISO 24444 and ISO 24443.

目的ALT-SPF联盟根据ISO 24444:2019进行了一项国际循环试验,以表征非侵入性方法作为基于红斑的防晒系数(SPF)测试的替代方法。方法基于多λ LED光源的混合漫反射光谱(HDRS)利用皮肤上的体内反射光谱测定防晒霜的体内吸收光谱,并将其与ISO 24443:2019中以薄膜透射方式测量的各自体外吸收光谱融合。作为ALT-SPF联盟倡议的一部分,在四个欧洲实验室对64个样本进行了盲法研究。在进一步改进方法后,对16个样本进行盲法再评价。由独立的统计机构评估新方法的5个统计可接受标准,将所得结果与SPF和UVA-PF的参考方法进行比较。结果初步的ALT-SPF研究1表明,偏倚标准是可以接受的,但重复性和实验室间变异性有待进一步改进。重新评价研究2表明,可重复性和实验室间变异性可显著改善。仅使用n = 10名志愿者,并基于初始ALT-SPF研究1的数据进行偏倚校正,重新评估研究2的SPF结果接近ALT-SPF研究标准1(可重复性)的接受标准,仅超过金标准性能定义的极限11%。UVA-PF结果在接受标准的接受范围内,除了标准3处于“几乎满足”的范围。重新评价研究表明,该方法的精密度与金标准方法ISO 24444和ISO 24443相当。结论本研究表明,LED-HDRS方法能够提供合理的无创SPF和UVA-PF结果,其性能与参考方法接近。因此,它可以作为现行标准ISO 24444和ISO 24443的替代方法提出。
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引用次数: 0
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International Journal of Cosmetic Science
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