Pub Date : 2024-12-12eCollection Date: 2024-01-01DOI: 10.1155/ijod/8260559
Bao-Ngoc Thi Nguyen, Chi-Tam Nguyen-Le, Bich-Ly Thi Nguyen, Son Hoang Le
Objective: The correlation between anatomy features of impacted mandibular third molars (M3Ms) and prevalence of pericoronitis was only interpreted using univariate analysis. This study investigated this correlation using multivariable analysis to determine the relationship between pericoronitis prevalence and the M3Ms' anatomical features. Methods: This cross-sectional study recruited 245 patients with 338 impacted M3Ms. One researcher collected participants' demographic characteristics such as sexes, age, side, and pericoronitis condition. The radiographic characteristics of M3Ms, including eruption direction and impacted levels according to Pell-Gregory classification, were assessed based on their orthopantomograms. Initially, univariate analyses were used to determine potential demographic and radiographic factors that correlated to pericoronitis. These factors were, then, analyzes using Firth's logistic regression. Results: No significant difference was found between non- and pericoronitis groups about sexes, age, side (p > 0.05). The univariate analyses showed that proportion of vertical impacted levels and eruption direction of M3Ms were significantly different between non- and pericoronitis groups. Firth's logistic regression analysis indicated that M3Ms with impacted level A were more likely to suffer from pericoronitis than ones at level B (odds ratio (OR) = 3.34), wheraes M3Ms impacted level II had higher risk of pericorinitis than ones at level I (OR = 1.63). Vertical M3Ms were more likely to develop pericoronitis than horizontal (OR = 5.78) ones. Conclusion: M3M angulation and impacted level are significant factors relating to pericoronitis prevalence. M3Ms with vertical eruption, vertically level A, and horizontally level B are more likely to have pericoronitis.
{"title":"Multivariable Analysis of Correlation Between Anatomical Features of Mandibular Third Molars and Pericoronitis.","authors":"Bao-Ngoc Thi Nguyen, Chi-Tam Nguyen-Le, Bich-Ly Thi Nguyen, Son Hoang Le","doi":"10.1155/ijod/8260559","DOIUrl":"10.1155/ijod/8260559","url":null,"abstract":"<p><p><b>Objective:</b> The correlation between anatomy features of impacted mandibular third molars (M3Ms) and prevalence of pericoronitis was only interpreted using univariate analysis. This study investigated this correlation using multivariable analysis to determine the relationship between pericoronitis prevalence and the M3Ms' anatomical features. <b>Methods:</b> This cross-sectional study recruited 245 patients with 338 impacted M3Ms. One researcher collected participants' demographic characteristics such as sexes, age, side, and pericoronitis condition. The radiographic characteristics of M3Ms, including eruption direction and impacted levels according to Pell-Gregory classification, were assessed based on their orthopantomograms. Initially, univariate analyses were used to determine potential demographic and radiographic factors that correlated to pericoronitis. These factors were, then, analyzes using Firth's logistic regression. <b>Results:</b> No significant difference was found between non- and pericoronitis groups about sexes, age, side (<i>p</i> > 0.05). The univariate analyses showed that proportion of vertical impacted levels and eruption direction of M3Ms were significantly different between non- and pericoronitis groups. Firth's logistic regression analysis indicated that M3Ms with impacted level A were more likely to suffer from pericoronitis than ones at level B (odds ratio (OR) = 3.34), wheraes M3Ms impacted level II had higher risk of pericorinitis than ones at level I (OR = 1.63). Vertical M3Ms were more likely to develop pericoronitis than horizontal (OR = 5.78) ones. <b>Conclusion:</b> M3M angulation and impacted level are significant factors relating to pericoronitis prevalence. M3Ms with vertical eruption, vertically level A, and horizontally level B are more likely to have pericoronitis.</p>","PeriodicalId":13947,"journal":{"name":"International Journal of Dentistry","volume":"2024 ","pages":"8260559"},"PeriodicalIF":1.9,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11658846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-05eCollection Date: 2024-01-01DOI: 10.1155/ijod/8227152
Shabnam Milani, Mehdi Shahrabi, Nazanin M-Abdi
Objectives: This study aimed to assess the effect of temperature of 1.23% acidulated phosphate fluoride (APF) gel on enamel microhardness of permanent teeth. Materials and Methods: This in vitro experimental study was conducted on 36 surgically extracted impacted human third molars. The teeth were mounted in acrylic resin and mesiodistally sectioned into buccal and lingual halves by a high-speed cutting saw. The buccal halves of the teeth were polished with silicone carbide abrasive paper and subjected to pH cycling (8 h of immersion in demineralizing agent and 16 h of immersion in remineralizing agent) for 10 days. Their primary enamel microhardness was subsequently measured by a Vickers microhardness tester. The specimens were then randomly assigned to three groups (n = 12) for application of 1.23% APF gel at 4, 25, and 37°C for 4 min. The specimens were then rinsed and incubated for 24 h. Their secondary enamel microhardness was subsequently measured, and the change in microhardness of the specimens was calculated and analyzed by one-way analysis of variance (ANOVA; α = 0.05). Results: The mean percentage of change in microhardness was -0.73% ± 16.8%, -5.28% ± 27.32%, and -7.27% ± 32.28% following the application of APF gel at 4, 25, and 37°C, respectively. The difference among the three groups was not significant regarding the percentage of change in microhardness (p > 0.05). Conclusion: Increasing the temperature of 1.23% APF gel from 4 to 25°C and 37°C did not cause a significant change in enamel microhardness of permanent teeth.
{"title":"Effect of Temperature of 1.23% Acidulated Phosphate Fluoride Gel on Enamel Microhardness of Permanent Teeth.","authors":"Shabnam Milani, Mehdi Shahrabi, Nazanin M-Abdi","doi":"10.1155/ijod/8227152","DOIUrl":"10.1155/ijod/8227152","url":null,"abstract":"<p><p><b>Objectives:</b> This study aimed to assess the effect of temperature of 1.23% acidulated phosphate fluoride (APF) gel on enamel microhardness of permanent teeth. <b>Materials and Methods:</b> This in vitro experimental study was conducted on 36 surgically extracted impacted human third molars. The teeth were mounted in acrylic resin and mesiodistally sectioned into buccal and lingual halves by a high-speed cutting saw. The buccal halves of the teeth were polished with silicone carbide abrasive paper and subjected to pH cycling (8 h of immersion in demineralizing agent and 16 h of immersion in remineralizing agent) for 10 days. Their primary enamel microhardness was subsequently measured by a Vickers microhardness tester. The specimens were then randomly assigned to three groups (<i>n</i> = 12) for application of 1.23% APF gel at 4, 25, and 37°C for 4 min. The specimens were then rinsed and incubated for 24 h. Their secondary enamel microhardness was subsequently measured, and the change in microhardness of the specimens was calculated and analyzed by one-way analysis of variance (ANOVA; <i>α</i> = 0.05). <b>Results:</b> The mean percentage of change in microhardness was -0.73% ± 16.8%, -5.28% ± 27.32%, and -7.27% ± 32.28% following the application of APF gel at 4, 25, and 37°C, respectively. The difference among the three groups was not significant regarding the percentage of change in microhardness (<i>p</i> > 0.05). <b>Conclusion:</b> Increasing the temperature of 1.23% APF gel from 4 to 25°C and 37°C did not cause a significant change in enamel microhardness of permanent teeth.</p>","PeriodicalId":13947,"journal":{"name":"International Journal of Dentistry","volume":"2024 ","pages":"8227152"},"PeriodicalIF":1.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-05eCollection Date: 2024-01-01DOI: 10.1155/ijod/4990295
Francesco Inchingolo, Angelo Michele Inchingolo, Giulia Latini, Elisabetta de Ruvo, Merigrazia Campanelli, Andrea Palermo, Massimo Del Fabbro, Marco Di Blasio, Alessio Danilo Inchingolo, Gianna Dipalma
Objective: Bone regeneration plays a pivotal role in modern oral surgery, particularly in facilitating successful implant-prosthetic rehabilitation. This systematic review explores the regenerative potential of growth factors, such as platelet-rich fibrin (PRF) and concentrated growth factors (CGFs), when combined with various types of scaffolds in bone augmentation procedures, including guided bone regeneration, split crest, sinus lift (SL), and alveolar ridge preservation. Method: A comprehensive search strategy yielded 18 relevant studies, which were analyzed for bone formation and stabilization outcomes. Results: Results indicate that PRF enhances bone regeneration and stabilization in SL and ridge augmentation procedures, while CGFs facilitate surgical techniques and augment bone. However, some studies did not report significant differences. Growth factors also demonstrate benefits in wound healing, reducing bone resorption, and enhancing socket preservation. Conclusion: Despite valuable insights, further research is needed to comprehensively understand the characteristics of growth factors in various surgical interventions, ensuring informed decision-making in bone regeneration surgery.
{"title":"Guided Bone Regeneration: CGF and PRF Combined With Various Types of Scaffolds-A Systematic Review.","authors":"Francesco Inchingolo, Angelo Michele Inchingolo, Giulia Latini, Elisabetta de Ruvo, Merigrazia Campanelli, Andrea Palermo, Massimo Del Fabbro, Marco Di Blasio, Alessio Danilo Inchingolo, Gianna Dipalma","doi":"10.1155/ijod/4990295","DOIUrl":"10.1155/ijod/4990295","url":null,"abstract":"<p><p><b>Objective:</b> Bone regeneration plays a pivotal role in modern oral surgery, particularly in facilitating successful implant-prosthetic rehabilitation. This systematic review explores the regenerative potential of growth factors, such as platelet-rich fibrin (PRF) and concentrated growth factors (CGFs), when combined with various types of scaffolds in bone augmentation procedures, including guided bone regeneration, split crest, sinus lift (SL), and alveolar ridge preservation. <b>Method:</b> A comprehensive search strategy yielded 18 relevant studies, which were analyzed for bone formation and stabilization outcomes. <b>Results:</b> Results indicate that PRF enhances bone regeneration and stabilization in SL and ridge augmentation procedures, while CGFs facilitate surgical techniques and augment bone. However, some studies did not report significant differences. Growth factors also demonstrate benefits in wound healing, reducing bone resorption, and enhancing socket preservation. <b>Conclusion:</b> Despite valuable insights, further research is needed to comprehensively understand the characteristics of growth factors in various surgical interventions, ensuring informed decision-making in bone regeneration surgery.</p>","PeriodicalId":13947,"journal":{"name":"International Journal of Dentistry","volume":"2024 ","pages":"4990295"},"PeriodicalIF":1.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-27eCollection Date: 2024-01-01DOI: 10.1155/ijod/8888815
Yi-Chu Wu, Fernanda L Schumacher, Dimitris N Tatakis
Objective: Despite the plethora of published periodontal interventional and experimental studies, there are no reports evaluating the experience of the participants as a research subject or their willingness for repeat participation in a similar study. This study aimed to determine the participants' experience and willingness to participate again in periodontal experimental studies and to explore factors that affect the participants' research experience. Materials and Methods: Questionnaires from four completed experimental wound healing studies with 76 total participants were extracted and analyzed. The participants answered the same specific questions at their last study visit. The questions asked were "overall experience in the study" and "willingness to participate in the study again," with each question providing five levels/possible answers. Questionnaires also provided an opportunity for participants to offer open-ended comments about their participation. Frequency distribution and logistic regression analyses were performed to evaluate the association between the participant's responses and study characteristics. Results: All 76 participants answered the specific questions. Overall, 88.2% of participants had a positive experience from their research participation, and 65.8% of them would participate again in such a study. Of the 76 participants, 50 were in studies that included multiple (≥2) experimental wounds, while 26 received only a single experimental wound. Participation experience was negatively associated with the number of wounds received (p < 0.001), while willingness to participate again was positively associated with participation experience (p < 0.001). Conclusions: Within the present study limitations, volunteers participating in periodontal experimental studies have an overall positive experience and express willingness for repeat participation.
{"title":"Participant Experience of Taking Part in Periodontal Experimental Studies.","authors":"Yi-Chu Wu, Fernanda L Schumacher, Dimitris N Tatakis","doi":"10.1155/ijod/8888815","DOIUrl":"10.1155/ijod/8888815","url":null,"abstract":"<p><p><b>Objective:</b> Despite the plethora of published periodontal interventional and experimental studies, there are no reports evaluating the experience of the participants as a research subject or their willingness for repeat participation in a similar study. This study aimed to determine the participants' experience and willingness to participate again in periodontal experimental studies and to explore factors that affect the participants' research experience. <b>Materials and Methods:</b> Questionnaires from four completed experimental wound healing studies with 76 total participants were extracted and analyzed. The participants answered the same specific questions at their last study visit. The questions asked were \"overall experience in the study\" and \"willingness to participate in the study again,\" with each question providing five levels/possible answers. Questionnaires also provided an opportunity for participants to offer open-ended comments about their participation. Frequency distribution and logistic regression analyses were performed to evaluate the association between the participant's responses and study characteristics. <b>Results:</b> All 76 participants answered the specific questions. Overall, 88.2% of participants had a positive experience from their research participation, and 65.8% of them would participate again in such a study. Of the 76 participants, 50 were in studies that included multiple (≥2) experimental wounds, while 26 received only a single experimental wound. Participation experience was negatively associated with the number of wounds received (<i>p</i> < 0.001), while willingness to participate again was positively associated with participation experience (<i>p</i> < 0.001). <b>Conclusions:</b> Within the present study limitations, volunteers participating in periodontal experimental studies have an overall positive experience and express willingness for repeat participation.</p>","PeriodicalId":13947,"journal":{"name":"International Journal of Dentistry","volume":"2024 ","pages":"8888815"},"PeriodicalIF":1.9,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The impact of the deep margin elevation (DME) technique and its associated materials on the stress distribution in ceramic endocrowns remains to be fully understood. This finite element analysis (FEA) aimed to assess the effects of flowable composite and resin-modified glass ionomer (RMGI) as DME materials on the maximum Von Mises stress (VMS) values and overall stress distribution within ceramic endocrowns and the surrounding tooth structure. Materials and Methods: A mandibular molar featuring a class II mesio occlusal (MO) cavity with the gingival margin of the mesial cavity positioned 2 mm below the cementoenamel junction (CEJ) was prepared and scanned using a Medit i500 scanner. The digital file was then transferred to computer-aided design (CAD) software to create the models. The study generated four scenarios: an intact tooth model (model of intact tooth (MIT)), a prepared tooth model without a DME layer (model without DME (MWD)), a model with a 2 mm DME layer using composite material (model with DME of composite (MDC)), and a model employing RMGI (model with DME of RMGI (MDR)). Stress distribution under axial loads was evaluated based on the Von Mises criterion. Results: The MIT model demonstrated the highest stress concentration at the CEJ region yet exhibited lower stress levels than others. The MWD model showed the highest stress levels. No significant differences in stress distribution patterns were observed between the MDR and MDC models. All models displayed similar stress distributions in the bone. Conclusion: Regardless of the material used, incorporating a DME layer in cavities extending below the CEJ is advisable to achieve uniform stress distribution. Minimizing tooth preparation and preserving tooth structure are recommended. Clinical Significance: Employing a DME layer in cavities with margins below the CEJ is beneficial for reducing stress, irrespective of the material choice.
目的:深缘提升技术(deep margin elevation, DME)及其相关材料对陶瓷内冠应力分布的影响尚不清楚。本文通过有限元分析(FEA)研究了可流动复合材料和树脂改性玻璃离聚体(RMGI)作为DME材料对陶瓷内冠和周围牙齿结构内最大Von Mises应力(VMS)值和总应力分布的影响。材料和方法:制备一颗具有II类近中牙合(MO)腔的下颌磨牙,近中牙合腔的龈缘位于牙骨质-牙釉质交界处(CEJ)下方2mm处,并使用Medit i500扫描仪进行扫描。然后将数字文件传输到计算机辅助设计(CAD)软件中以创建模型。本研究生成了四种场景:完整牙齿模型(完整牙齿模型(MIT))、没有DME层的制备牙齿模型(没有DME层的模型(MWD))、使用复合材料的2 mm DME层模型(复合材料的DME模型(MDC))和使用RMGI的模型(RMGI的DME模型(MDR))。基于Von Mises准则对轴向载荷作用下的应力分布进行了评价。结果:MIT模型在CEJ区域表现出最高的应力集中,但其应力水平低于其他模型。MWD模型显示出最高的应力水平。应力分布模式在MDR和MDC模型之间没有显著差异。所有模型在骨骼中显示出相似的应力分布。结论:无论使用何种材料,在延伸到CEJ以下的空腔中加入二甲醚层是可取的,可以实现均匀的应力分布。建议尽量减少牙齿的准备工作,保持牙齿的结构。临床意义:无论选择何种材料,在边缘低于CEJ的空腔中使用二甲醚层都有利于减轻应力。
{"title":"Influence of Deep Margin Elevation Technique With Two Restorative Materials on Stress Distribution of e.max Endocrown Restorations: A Finite Element Analysis.","authors":"Fariba MahmoudiYamchi, Mahdi Abbasi, Faezeh Atri, Elham Ahmadi","doi":"10.1155/ijod/6753069","DOIUrl":"10.1155/ijod/6753069","url":null,"abstract":"<p><p><b>Objective:</b> The impact of the deep margin elevation (DME) technique and its associated materials on the stress distribution in ceramic endocrowns remains to be fully understood. This finite element analysis (FEA) aimed to assess the effects of flowable composite and resin-modified glass ionomer (RMGI) as DME materials on the maximum Von Mises stress (VMS) values and overall stress distribution within ceramic endocrowns and the surrounding tooth structure. <b>Materials and Methods:</b> A mandibular molar featuring a class II mesio occlusal (MO) cavity with the gingival margin of the mesial cavity positioned 2 mm below the cementoenamel junction (CEJ) was prepared and scanned using a Medit i500 scanner. The digital file was then transferred to computer-aided design (CAD) software to create the models. The study generated four scenarios: an intact tooth model (model of intact tooth (MIT)), a prepared tooth model without a DME layer (model without DME (MWD)), a model with a 2 mm DME layer using composite material (model with DME of composite (MDC)), and a model employing RMGI (model with DME of RMGI (MDR)). Stress distribution under axial loads was evaluated based on the Von Mises criterion. <b>Results:</b> The MIT model demonstrated the highest stress concentration at the CEJ region yet exhibited lower stress levels than others. The MWD model showed the highest stress levels. No significant differences in stress distribution patterns were observed between the MDR and MDC models. All models displayed similar stress distributions in the bone. <b>Conclusion:</b> Regardless of the material used, incorporating a DME layer in cavities extending below the CEJ is advisable to achieve uniform stress distribution. Minimizing tooth preparation and preserving tooth structure are recommended. <b>Clinical Significance:</b> Employing a DME layer in cavities with margins below the CEJ is beneficial for reducing stress, irrespective of the material choice.</p>","PeriodicalId":13947,"journal":{"name":"International Journal of Dentistry","volume":"2024 ","pages":"6753069"},"PeriodicalIF":1.9,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617049/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-25eCollection Date: 2024-01-01DOI: 10.1155/ijod/4285984
Emma Rachmawati, Shinta Asarina, Gabriel Bagus Kennardi, Akeyla Tabina Tawangalun, Candra Arumimaniyah, Kartika Indah Sari, Hening Tjaturina Pramesti, Ratu Safitri, Ani Melani Maskoen
Thermophilic bacteria living in extreme areas with high temperatures are capable of producing secondary metabolites, such as antimicrobial peptides (AMPs). AMPs are stable at high temperatures and show good antibacterial activity. Therefore, this study aimed to identify thermophilic bacteria from the crater of Mount Tangkuban Perahu around West Java and assess antibacterial effectiveness of AMPs against Streptococcus mutans, which contribute to oral biofilm formation. The isolate obtained was identified using 16S ribosomal ribonucleic acid (rRNA) gene sequencing, and the supernatant of the isolate was tested against S. mutans American Type Culture Collection (ATCC) 25175 using the disc assay method. To determine AMPs-coding genes, its genome was uploaded to antibiotic and secondary metabolite analysis shell (antiSMASH) 5.0.0 platform and biofilm inhibition was tested using the microtiter plate technique (with a 96-well bottom). Subsequently, the results were assessed using a microplate reader operating at 595 nm wavelength. The isolate was identified as Geobacillus subterraneus, with antibacterial activity against S. mutans, and produced an inhibition zone of 8.40 mm at an optimum pH of 8. The output of AMPs-coding gene showed that AMPs of the isolate were a member of the lanthipeptide class I, or bacteriocin-I group. AMPs of G. subterraneus suppressed the growth of S. mutans biofilm at a supernatant concentration of 5%, with the lowest optical density (OD) value of 0.061 and the highest percentage of biofilm growth inhibition at 28.24%. Based on the results, G. subterraneus derived from the crater of Mount Tangkuban Perahu showed potent antibacterial properties against S. mutans, making it a promising novel S. mutans anti-biofilm candidate.
生活在极端高温地区的嗜热细菌能够产生次生代谢产物,如抗菌肽(AMPs)。amp在高温下稳定,具有良好的抗菌活性。因此,本研究旨在鉴定来自西爪哇附近的唐库班佩拉胡火山火山口的嗜热细菌,并评估amp对促进口腔生物膜形成的变形链球菌的抗菌效果。采用16S核糖体核糖核酸(rRNA)基因测序对分离物进行鉴定,并用圆盘法对分离物的上清液与S. mutans American Type Culture Collection (ATCC) 25175进行检测。为了确定amps编码基因,将其基因组上传到抗生素和次级代谢物分析shell (antiSMASH) 5.0.0平台,采用微滴板技术(96孔底)检测生物膜抑制作用。随后,使用595 nm波长的微孔板读取器对结果进行评估。经鉴定,该菌株为地下地杆菌,对变形链球菌具有抑菌活性,在最适pH为8时产生了8.40 mm的抑菌带。编码基因输出结果表明,该分离物的AMPs属I类细菌肽,即细菌素-I族。在上清浓度为5%时,地下G. AMPs对变形链球菌生物膜的生长有抑制作用,其光密度(OD)值最低为0.061,生物膜生长抑制率最高为28.24%。结果表明,产自Perahu Tangkuban山火山口的地下葡萄球菌对变形链球菌具有较强的抗菌活性,是一种很有前景的新型变形链球菌抗生物膜候选物。
{"title":"Isolation of Thermophilic Bacteria <i>Geobacillus subterraneus</i> From Mount Tangkuban Perahu and the Novelty as a Candidate for <i>Streptococcus mutans</i> Anti-Biofilm.","authors":"Emma Rachmawati, Shinta Asarina, Gabriel Bagus Kennardi, Akeyla Tabina Tawangalun, Candra Arumimaniyah, Kartika Indah Sari, Hening Tjaturina Pramesti, Ratu Safitri, Ani Melani Maskoen","doi":"10.1155/ijod/4285984","DOIUrl":"10.1155/ijod/4285984","url":null,"abstract":"<p><p>Thermophilic bacteria living in extreme areas with high temperatures are capable of producing secondary metabolites, such as antimicrobial peptides (AMPs). AMPs are stable at high temperatures and show good antibacterial activity. Therefore, this study aimed to identify thermophilic bacteria from the crater of Mount Tangkuban Perahu around West Java and assess antibacterial effectiveness of AMPs against <i>Streptococcus mutans</i>, which contribute to oral biofilm formation. The isolate obtained was identified using 16S ribosomal ribonucleic acid (rRNA) gene sequencing, and the supernatant of the isolate was tested against <i>S. mutans</i> American Type Culture Collection (ATCC) 25175 using the disc assay method. To determine AMPs-coding genes, its genome was uploaded to antibiotic and secondary metabolite analysis shell (antiSMASH) 5.0.0 platform and biofilm inhibition was tested using the microtiter plate technique (with a 96-well bottom). Subsequently, the results were assessed using a microplate reader operating at 595 nm wavelength. The isolate was identified as <i>Geobacillus subterraneus</i>, with antibacterial activity against <i>S. mutans</i>, and produced an inhibition zone of 8.40 mm at an optimum pH of 8. The output of AMPs-coding gene showed that AMPs of the isolate were a member of the lanthipeptide class I, or bacteriocin-I group. AMPs of <i>G. subterraneus</i> suppressed the growth of <i>S. mutans</i> biofilm at a supernatant concentration of 5%, with the lowest optical density (OD) value of 0.061 and the highest percentage of biofilm growth inhibition at 28.24%. Based on the results, <i>G. subterraneus</i> derived from the crater of Mount Tangkuban Perahu showed potent antibacterial properties against <i>S. mutans</i>, making it a promising novel <i>S. mutans</i> anti-biofilm candidate.</p>","PeriodicalId":13947,"journal":{"name":"International Journal of Dentistry","volume":"2024 ","pages":"4285984"},"PeriodicalIF":1.9,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11614514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The study of working conditions and the quality of life (QoL) of medical workers has not lost its relevance worldwide. This highlights the need to understand the many aspects of life that may characterize physician performance and satisfaction with working conditions. This is extremely important for providing quality and safe medical care. This research aims to study dentists' lifestyles and working conditions in the Republic of Kazakhstan and the impact of these indicators on their QoL. Methods: This article presents the results of a cross-sectional survey of a sociological questionnaire conducted with the participation of 254 dentists in Kazakhstan. Results: Only a third of dentists follow a healthy lifestyle. In total, 39% of dentists currently smoke, and 24% drink alcohol. Dentists with more than 20 years of experience have a worse QoL and have the lowest scores in all domains compared to dentists with less experience. The lowest score was scored for mental health (mean = 51.81). On all scales, dentists who worked more than 40 h a week reported decreased QoL; the lowest score was in the "mental health" domain (mean = 52.80). Conclusion: The QoL of dentists in Kazakhstan is negatively affected by their working hours and long working experience. Measures are necessary to improve their working conditions, work schedule planning, and mental health.
{"title":"Lifestyle, Working Conditions, and Quality of Life Among Dentists in Kazakhstan.","authors":"Azhar Syzdykova, Karlygash Toguzbayeva, Aiman Syzdykova, Danara Bekkazinova, Ainur B Qumar, Akmaral Abikulova, Aigulsum Izekenova","doi":"10.1155/ijod/4290036","DOIUrl":"10.1155/ijod/4290036","url":null,"abstract":"<p><p><b>Background:</b> The study of working conditions and the quality of life (QoL) of medical workers has not lost its relevance worldwide. This highlights the need to understand the many aspects of life that may characterize physician performance and satisfaction with working conditions. This is extremely important for providing quality and safe medical care. This research aims to study dentists' lifestyles and working conditions in the Republic of Kazakhstan and the impact of these indicators on their QoL. <b>Methods:</b> This article presents the results of a cross-sectional survey of a sociological questionnaire conducted with the participation of 254 dentists in Kazakhstan. <b>Results:</b> Only a third of dentists follow a healthy lifestyle. In total, 39% of dentists currently smoke, and 24% drink alcohol. Dentists with more than 20 years of experience have a worse QoL and have the lowest scores in all domains compared to dentists with less experience. The lowest score was scored for mental health (mean = 51.81). On all scales, dentists who worked more than 40 h a week reported decreased QoL; the lowest score was in the \"mental health\" domain (mean = 52.80). <b>Conclusion:</b> The QoL of dentists in Kazakhstan is negatively affected by their working hours and long working experience. Measures are necessary to improve their working conditions, work schedule planning, and mental health.</p>","PeriodicalId":13947,"journal":{"name":"International Journal of Dentistry","volume":"2024 ","pages":"4290036"},"PeriodicalIF":1.9,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11614501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-22eCollection Date: 2024-01-01DOI: 10.1155/ijod/1257136
Manjusha Nambiar, Bhavya Shetty, Ibrahim Fazal, Safiya Fatima Khan, Mehul A Shah, Vignesh Kamath, Shahaziya Faruk, Vaishnavi Jalaj, Sowmya N
Objective: In office methods for immediate relief of dentinal hypersensitivity (DH) has long been an area of research. This study compared the efficacy of 660 nm diode laser, 980 nm diode laser, and amorphous calcium phosphate-casein phosphopeptide (ACP-CPP) agent in the treatment of DH. Materials and Methods: A total of 39 patients with minimum three hypersensitive teeth in at least one quadrant were selected and randomly divided into three groups; Group A, B, and C patients were treated by 660 nm diode laser, 980 nm diode laser, and ACP-CPP agent, respectively. All the hypersensitive teeth were stimulated with tactile; thermal and air stimuli, and the pretreatment and posttreatment hypersensitivity scores were evaluated with the numeric rating scale (NRS) at baseline, 30 min, 1 week, 1 month, 3 months, and 6 months. The Shapiro-Wilk test was used to determine the uniformity of the data. The Chi-square (χ2) test of significance was used to compare proportions. For intergroup comparison, the Kruskal-Wallis test and the Mann-Whitney U test were utilized. Results: At 30 min and 1 week, the 980 nm laser was more effective than the 660 nm laser, but there was no statistically significant difference between the two groups at 1, 3, and 6 months. Throughout the trial period, the 660 nm and 980 nm diode lasers were more effective than the ACP-CPP agent in lowering DH. Conclusions: 660 nm diode laser, 980 nm diode laser, and ACP-CPP agent showed positive prospective as effective desensitizers when utilized as a clinical procedure.
{"title":"Effectiveness of Two Wavelengths of Diode Laser and Amorphous Calcium Phosphate-Casein Phosphopeptide Mousse in the Treatment of Dentinal Hypersensitivity: A Randomized Clinical Study.","authors":"Manjusha Nambiar, Bhavya Shetty, Ibrahim Fazal, Safiya Fatima Khan, Mehul A Shah, Vignesh Kamath, Shahaziya Faruk, Vaishnavi Jalaj, Sowmya N","doi":"10.1155/ijod/1257136","DOIUrl":"https://doi.org/10.1155/ijod/1257136","url":null,"abstract":"<p><p><b>Objective:</b> In office methods for immediate relief of dentinal hypersensitivity (DH) has long been an area of research. This study compared the efficacy of 660 nm diode laser, 980 nm diode laser, and amorphous calcium phosphate-casein phosphopeptide (ACP-CPP) agent in the treatment of DH. <b>Materials and Methods:</b> A total of 39 patients with minimum three hypersensitive teeth in at least one quadrant were selected and randomly divided into three groups; Group A, B, and C patients were treated by 660 nm diode laser, 980 nm diode laser, and ACP-CPP agent, respectively. All the hypersensitive teeth were stimulated with tactile; thermal and air stimuli, and the pretreatment and posttreatment hypersensitivity scores were evaluated with the numeric rating scale (NRS) at baseline, 30 min, 1 week, 1 month, 3 months, and 6 months. The Shapiro-Wilk test was used to determine the uniformity of the data. The Chi-square (<i>χ</i> <sup>2</sup>) test of significance was used to compare proportions. For intergroup comparison, the Kruskal-Wallis test and the Mann-Whitney <i>U</i> test were utilized. <b>Results:</b> At 30 min and 1 week, the 980 nm laser was more effective than the 660 nm laser, but there was no statistically significant difference between the two groups at 1, 3, and 6 months. Throughout the trial period, the 660 nm and 980 nm diode lasers were more effective than the ACP-CPP agent in lowering DH. <b>Conclusions:</b> 660 nm diode laser, 980 nm diode laser, and ACP-CPP agent showed positive prospective as effective desensitizers when utilized as a clinical procedure.</p>","PeriodicalId":13947,"journal":{"name":"International Journal of Dentistry","volume":"2024 ","pages":"1257136"},"PeriodicalIF":1.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11608297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-15eCollection Date: 2024-01-01DOI: 10.1155/2024/2540174
Ali Dhahee Malallah, Nadia H Hasan, Mohammed Hazim Qasim
Aims: Careful selection of materials and resin cement shade can minimize color changes in laminate veneers. This study aimed to evaluate the influence of two ceramic material types, lithium disilicate glass-ceramic material (Ivoclar, Schaan/Liechtenstein; IPS e.max), computer aided design (CAD) and IPS e.max Press, and four different resin cement shades on color changes in lithium disilicate ceramic laminate veneers. Methods: Forty extracted human maxillary first premolars were prepared to receive a laminate veneer. Optical scanning and digital designing were used to prepare 20 IPS e.max CAD and 20 IPS e.max Press veneer samples. These samples were divided into four groups based on resin cement shade and material: Group CAD A1 : (IPS e.max CAD with A1 cement shade), Group CAD T : (IPS e.max CAD with translucent cement shade), Group CAD M : (IPS e.max CAD with milky bright cement shade), Group CAD B1 : (IPS e.max CAD with B1 cement shade) and the same divisions for IPS e.max Press. Before cementation, color measurements were obtained using precision colorimeter NR110. After cementation, the ∆E (color change) value was recorded and tabulated. One-way analysis of variance (ANOVA) and Duncan's post hoc test were used to evaluate the influence of ceramic material types and cement shade on ∆E. Results: There were significant differences between the IPS e.max CAD and e.max Press veneers for the four tested cement shades. The highest color change was observed in press M veneers (8.84 ± 0.63931) while the lowest color change was observed in CAD M veneers (0.5 ± 0.16371). There were significant differences in color change based on cement shade, with B1 showing the greatest change (8.84 ± 0.47440 for Press veneers and 2.3 ± 0.11992 for CAD veneers). Conclusions: Different shades of resin cements and different manufacturing techniques produce different levels of color changes therefore careful selection of materials and cement shade can minimize color changes in laminate veneers.
{"title":"Influence of Ceramic Material Type and Cement Shade on the Translucency of Lithium Disilicate Ceramic Veneers.","authors":"Ali Dhahee Malallah, Nadia H Hasan, Mohammed Hazim Qasim","doi":"10.1155/2024/2540174","DOIUrl":"10.1155/2024/2540174","url":null,"abstract":"<p><p><b>Aims:</b> Careful selection of materials and resin cement shade can minimize color changes in laminate veneers. This study aimed to evaluate the influence of two ceramic material types, lithium disilicate glass-ceramic material (Ivoclar, Schaan/Liechtenstein; IPS e.max), computer aided design (CAD) and IPS e.max Press, and four different resin cement shades on color changes in lithium disilicate ceramic laminate veneers. <b>Methods:</b> Forty extracted human maxillary first premolars were prepared to receive a laminate veneer. Optical scanning and digital designing were used to prepare 20 IPS e.max CAD and 20 IPS e.max Press veneer samples. These samples were divided into four groups based on resin cement shade and material: Group CAD A1 : (IPS e.max CAD with A1 cement shade), Group CAD T : (IPS e.max CAD with translucent cement shade), Group CAD M : (IPS e.max CAD with milky bright cement shade), Group CAD B1 : (IPS e.max CAD with B1 cement shade) and the same divisions for IPS e.max Press. Before cementation, color measurements were obtained using precision colorimeter NR110. After cementation, the <i>∆E</i> (color change) value was recorded and tabulated. One-way analysis of variance (ANOVA) and Duncan's post hoc test were used to evaluate the influence of ceramic material types and cement shade on <i>∆E</i>. <b>Results:</b> There were significant differences between the IPS e.max CAD and e.max Press veneers for the four tested cement shades. The highest color change was observed in press M veneers (8.84 ± 0.63931) while the lowest color change was observed in CAD M veneers (0.5 ± 0.16371). There were significant differences in color change based on cement shade, with B1 showing the greatest change (8.84 ± 0.47440 for Press veneers and 2.3 ± 0.11992 for CAD veneers). <b>Conclusions:</b> Different shades of resin cements and different manufacturing techniques produce different levels of color changes therefore careful selection of materials and cement shade can minimize color changes in laminate veneers.</p>","PeriodicalId":13947,"journal":{"name":"International Journal of Dentistry","volume":"2024 ","pages":"2540174"},"PeriodicalIF":1.9,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objectives: The presence of parents during dental treatment in children is a controversial concern in dental practice. This is because of conflicting views and practices regarding the presence of parents. Therefore, the objective of this study was to evaluate parental response to their presence/absence during their child's dental treatment and to determine the factors that would influence their decision. Methods: A cross-sectional survey questionnaire was administered to United Arab Emirates (UAE) parents. A total of 240 parents participated in the survey, which contained 15 questions that analyzed the participants' demographic details, dental procedures influencing parental separation, and factors influencing their desire to be present during their child's dental treatment. Results: Data were analyzed using descriptive statistics and chi-square tests (p < 0.05). The majority (78%) of the parents chose to stay with their children during dental treatment, with a higher prevalence of female parents. The results showed that more parents opted to be with their children during invasive procedures. Younger parents are more likely to stay with their children during dental treatment. The factors influencing parental presence/absence in the dental treatment room depended on the age and nationality of the parent and the type of dental procedure (p < 0.05). Conclusions: Dental practitioners must provide parents with sufficient opportunities to be present during their children's dental procedures. The factors influencing parental presence/absence should be considered before deciding whether to include or exclude parents in the dental treatment room.
{"title":"Understanding Parental Emotions: Children's Dental Visits and the Separation Experience.","authors":"Ruba Odeh, Carel Brigi, Tarun Walia, Raghad Hashim","doi":"10.1155/2024/5893717","DOIUrl":"10.1155/2024/5893717","url":null,"abstract":"<p><p><b>Objectives:</b> The presence of parents during dental treatment in children is a controversial concern in dental practice. This is because of conflicting views and practices regarding the presence of parents. Therefore, the objective of this study was to evaluate parental response to their presence/absence during their child's dental treatment and to determine the factors that would influence their decision. <b>Methods:</b> A cross-sectional survey questionnaire was administered to United Arab Emirates (UAE) parents. A total of 240 parents participated in the survey, which contained 15 questions that analyzed the participants' demographic details, dental procedures influencing parental separation, and factors influencing their desire to be present during their child's dental treatment. <b>Results:</b> Data were analyzed using descriptive statistics and chi-square tests (<i>p</i> < 0.05). The majority (78%) of the parents chose to stay with their children during dental treatment, with a higher prevalence of female parents. The results showed that more parents opted to be with their children during invasive procedures. Younger parents are more likely to stay with their children during dental treatment. The factors influencing parental presence/absence in the dental treatment room depended on the age and nationality of the parent and the type of dental procedure (<i>p</i> < 0.05). <b>Conclusions:</b> Dental practitioners must provide parents with sufficient opportunities to be present during their children's dental procedures. The factors influencing parental presence/absence should be considered before deciding whether to include or exclude parents in the dental treatment room.</p>","PeriodicalId":13947,"journal":{"name":"International Journal of Dentistry","volume":"2024 ","pages":"5893717"},"PeriodicalIF":1.9,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11581791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}