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Biochemical Analysis Based on Zinc Uptake of Chickpea (Cicer arietinum L.) Varieties Infected by Meloidogyne incognita 鹰嘴豆(Cicer arietinum L.)锌吸收的生化分析被不明丝虫病感染的品种
Pub Date : 2021-12-14 DOI: 10.9734/ijpss/2021/v33i2430767
Pranaya Pradhan, D. Nayak, Manaswini Mahapatra
The significant constraints in Chickpea (Cicer arietinum L.) production hampers a bit more than 14% global yield loss due to plant-parasitic nematodes. Root-knot nematode (Meloidogyne sp.) is an endoparasite and a significant species affecting the chickpea plant. So, the chemical basis of management is more cost-effective, and pest resurgence building is enhanced in the pathogen. So, ecological-based nematode management is requisite, which also is got hampered due to breeding for resistance against such plant-parasitic nematodes. This was the primary reason to conduct this experiment to enhance resistance in the chickpea plants based on Zinc uptake by using bioagent, Pseudomonas fluorescens alone or in combination. where Different treatments including nematode, bacterium, and chemicals were used sustaining the enhancement of disease resistance in chickpea cultivars, RSG 974, GG 5, GNG 2144. Zinc content of chickpea variety GNG 2144 was found the highest in treatment, when only bacterium (P. fluorescens) was inoculated, i.e., 3.14 mg/100g of root followed by GG 5, i.e., 2.79 mg/100g of root and RSG 974 was, i.e., 2.35 mg/100g of root respectively in a descending order. Application of P. fluorescence combined or alone gradually increased the Zn concentration in roots of chickpea plants compared to healthy check followed by chemical treated plants.
鹰嘴豆(Cicer arietinum L.)生产受到严重限制,导致全球因植物寄生线虫造成的产量损失略高于14%。根结线虫(Meloidogyne sp.)是影响鹰嘴豆植物的一种重要内寄生虫。因此,化学基础管理更具成本效益,并在病原体中加强了害虫的复生建设。因此,基于生态的线虫管理是必要的,但由于对这种植物寄生线虫的抗性育种而受到阻碍。因此,本试验旨在通过单独或联合使用荧光假单胞菌对锌的吸收来增强鹰嘴豆植株的抗性。其中,采用线虫、细菌和化学药剂等不同处理,持续增强了鹰嘴豆品种RSG 974、GG 5、GNG 2144的抗病性。鹰嘴豆品种GNG 2144的锌含量最高,仅接种细菌(P. fluorescens)时为3.14 mg/100g,其次为GG 5,为2.79 mg/100g, RSG 974为2.35 mg/100g。与先化学处理后健康对照相比,P.荧光联合或单独施用使鹰嘴豆植株根系锌浓度逐渐升高。
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引用次数: 0
Impact of Organic and Inorganic Sources of Nutrients on Root Architecture, Soil Microbial Biomass and Yield on Low Land Rice Ecosystem 有机和无机营养源对水稻根系构型、土壤微生物量和产量的影响
Pub Date : 2021-12-14 DOI: 10.9734/ijpss/2021/v33i2430773
V. R. Senthamizhkumaran, P. Santhy, D. Selvi, T. Kalaiselvi, K. Sabarinathan
To study the impact of vermicompost, arbuscular mycorrhizae and FYM application on the rice ecosystem at low land, a field experiment was conducted with rice CO(R) 51 at the Department of Soil Science and Agricultural Chemistry, Tamil Nadu Agricultural University in Coimbatore during the winter of 2020. The experiment was framed in Randomized Block Design comprising of 8 treatments viz., Recommended Dose of Fertilizer Soil Test Crop Response approach (T1), RDF 75 % + Farm Yard Manure @ 12.5 t ha-1 (T2), T2 + Seed treatment with Azospirillum and Phosphobacteria + Soil application of AM fungi (T3), RDF 75 % + Vermicompost @ 5 t ha-1 (T4), T4 + Seed treatment with Azospirillum and Phosphobacteria + Soil application of AM fungi (T5), FYM @ 12.5 t ha-1 + Seed treatment with Azospirillum and Phosphobacteria + Soil application of AM fungi (T6), Vermicompost @ 5 t ha-1+ Seed treatment with Azospirillum and Phosphobacteria + Soil application of AM fungi (T7) and Absolute control (T8) and replicated thrice. The maximum microbial population were registered in the plots that received integrated nutrient application of RDF 75 % STCR approach + Vermicompost 5 t ha-1 + seed treatment with Azospirillum and Phosphobacteria + Soil application of AM fungi. Rice root architecture has changed significantly as a result of mycorrhizal inoculation. Mycorrhizal rice plants have more root volume, length, and spread than plants without mycorrhizae. Nutrient retention and availability influenced the presence of microbial-mediated metabolic activities and nutrient transformations during crop growth. Bacteria, fungus, and actinomycetes became less abundant as the crop reached harvest. The population density of mycorrhizospheres that utilize both organic and inorganic fertilizers is higher. The treatments that received Vermicompost or FYM with Vesicular Arbuscular Mycorrhizae and Nitrogen, Phosphorous & Potassium fertilizers obtained the highest yields of rice grain and straw (6740 and 7840 kg ha-1) respectively, and it was clear that the combination of Vermicompost or FYM, VAM and along with NPK fertilizers produced significantly higher yields than their individual applications and absolute control.
为研究蚯蚓堆肥、丛集菌根和FYM施用对低洼地水稻生态系统的影响,于2020年冬季在哥印拜陀泰米尔纳德邦农业大学土壤科学与农业化学系以水稻CO(R) 51进行了田间试验。试验采用随机区组设计,包括8个处理,即肥料推荐剂量土壤试验作物反应法(T1)、RDF 75% +农场厩肥@ 12.5 t ha-1 (T2)、T2 +氮螺旋体和磷细菌种子处理+ AM真菌土壤施用(T3)、RDF 75% +蚯蚓堆肥@ 5 t ha-1 (T4)、T4 +氮螺旋体和磷细菌种子处理+ AM真菌土壤施用(T5)。FYM @ 12.5 t hm -1+氮螺旋体和磷细菌种子处理+土壤施用AM真菌(T6),蚯蚓堆肥@ 5 t hm -1+氮螺旋体和磷细菌种子处理+土壤施用AM真菌(T7)和绝对对照(T8),重复三次。采用RDF 75% STCR +蚯蚓堆肥5 t hm -1 +固氮螺旋菌和磷细菌种子处理+ AM真菌土壤施用的综合施肥方式,土壤微生物数量最多。接种菌根后,水稻根系结构发生了显著变化。有菌根的水稻植株比没有菌根的植株有更大的根体积、长度和扩展。养分的保留和有效性影响作物生长过程中微生物介导的代谢活动和养分转化的存在。随着作物丰收,细菌、真菌和放线菌数量减少。同时施用有机肥和无机肥的菌根圈种群密度较高。蚯蚓堆肥或FYM配泡状菌根和氮、磷、钾肥处理水稻籽粒和秸秆产量最高(分别为6740和7840 kg hm -1),蚯蚓堆肥或FYM、VAM配施氮磷钾肥的产量显著高于单独施用和绝对对照。
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引用次数: 0
Site Specific Nutrient Management Options for Achieving Higher Yields in Cotton and Chickpea under Rainfed Condition 在旱作条件下实现棉花和鹰嘴豆高产的定点营养管理选择
Pub Date : 2021-12-14 DOI: 10.9734/ijpss/2021/v33i2430769
S. Nayak, D. Balaguravaiah, K. Ramana, T. Giridharakrishna, P. Munirathnam, B. Reddy
A study was undertaken to delineation of spatial variability of soil fertility status in order to prepare soil available nutrient maps for improved productivity in different crops grown in the study area of Kurnool revenue division in Kurnool district of Andhra Pradesh state using remote sensing and GIS techniques. The Knowledge of spatial-variability is critical for site specific nutrient management in soil fertility to obtain higher yields. Soil sample (350) were collected from surface from 350 selected sites for preparing precise digital maps using point, line and polygon tools of the Geographic Information System (GIS) with ArcGIS software 10.3 was used for database creation and for creating the union of various thematic maps. The spatial variability maps were generated and delineated into different zones for N, P and K. Soil available Nitrogen, Phosphorus and Potassium spatial variability values generated from the thematic maps of Kurnool division were used to establish fertilizer recommendations for cotton in kharif and Chickpea in rabi during  2018-19 seasons. The recommended doses of Nitrogen (RDN) that worked for cotton were 401 to 450, 351 to 400, > 450 and < 350 kg ha-1 for the areas with Nitrogen availability of 140 to 210, 210 to 280, <140 and > 280 kg/ha, respectively. The Phosphorous fertilizer recommendation for soils with available P of < 30 kg/ha and > 30 kg/ha was figured out as > 250 kg ha-1 and < 250 kg ha-1, respectively. For soil available Potassium recorded 230 to 560, < 230 and > 560 kg/ha, the K recommendation was figured out as 301 to 400, > 401 and < 300 kg/ha, respectively. Recommended doses of Nitrogen (RDN) was worked out for chickpea were 51 to 75, > 76 and < 50 kg/ha for the areas with available N ranges of 184 to 280, < 184 and > 280 kg/ha, respectively. The Phosphatic fertilizer recommendation for soils of available P of < 23.5 and 23.5 to 40 kg/ha was figured out as > 200 kg/ha and 171 to 200 kg/ha, respectively. For the soil available potassium recorded 253 to 412, 413 to 570, < 253 and > 570 kg/ha, the K recommendation was figured out as 66 to 100, 31 to 65, > 100 and < 30 kg/ha, respectively.
利用遥感和地理信息系统技术,对安得拉邦库尔努尔地区库尔努尔收入区划研究区土壤肥力状况的空间变异性进行了研究,以编制土壤有效养分图,提高不同作物的生产力。空间变异性的知识对土壤肥力的特定地点养分管理至关重要,以获得更高的产量。利用地理信息系统(GIS)的点、线、多边形工具,利用ArcGIS软件(10.3)建立数据库和创建各专题地图的联合,从350个选定地点的地表采集土壤样品(350份),制作精确的数字地图。利用Kurnool分区专题图生成的土壤速效氮、磷、钾空间变异值,建立了2018-19季棉花和鹰嘴豆的施肥建议。氮素效价为140 ~ 210、210 ~ 280、280 kg/ha时,棉花推荐施氮量分别为401 ~ 450、351 ~ 400、> 450和< 350 kg/ha。计算出有效磷< 30 kg/ha和> 30 kg/ha土壤的磷肥推荐量分别为> 250 kg ha-1和< 250 kg ha-1。土壤速效钾为230 ~ 560、< 230和> 560 kg/ha时,推荐钾量分别为301 ~ 400、> 401和< 300 kg/ha。在有效氮含量为184 ~ 280、< 184和> 280 kg/ha的地区,鹰嘴豆的推荐施氮量分别为51 ~ 75、> 76和< 50 kg/ha。计算出有效磷< 23.5、23.5 ~ 40 kg/ha土壤磷肥推荐量分别为> 200 kg/ha和171 ~ 200 kg/ha。土壤速效钾为253 ~ 412、413 ~ 570、< 253和> 570 kg/ha时,K推荐值分别为66 ~ 100、31 ~ 65、> 100和< 30 kg/ha。
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引用次数: 0
Effect of Integrated Nutrient Management on Plant Growth and Tuber Yield of Dahlia (Dahlia variabilis) cv. Kenya White 综合养分管理对大丽花植株生长和块茎产量的影响肯尼亚的白色
Pub Date : 2021-12-13 DOI: 10.9734/ijpss/2021/v33i2430753
T. Raghunandan, J. Collis, S. Saravanan, K. Barman
The experiment was carried out at Experimental field, Department of Horticulture, Sam Higginbottom Institute of Agriculture Technology and Sciences [formerly known as Allahabad Agriculture Institute Deemed University, AAI-DU] during the Spring season 2014-2015. The experiment consists of Thirteen treatments viz., (T0) Control-RDF (100:120:100 N P K  kg ha-1,), (T1) 75% RDF + AZ@ 2.5 kg ha-1,, (T2) 75% RDF + FYM @ 10 ton ha-1, (T3) 75% RDF + VC@ 10 ton ha-1, (T4) 75% RDF + AZ @ 2.5 kg/ha +FYM @ 10 ton ha-1+VC @ 10 ton ha-1, (T5) 50% RDF +AZ@ 2.5 kg ha-1, (T6) 50% RDF + FYM@ 10 ton ha-1, (T7) 50% RDF + VC@ 10 ton ha-1, (T8) 50% RDF +  AZ @ 2.5 kg ha-1+FYM @ 10 ton ha-1+VC @ 10 ton ha-1, (T9) 25% RDF + AZ@ 2.5 kg ha-1, (T10) 25% RDF + FYM@ 10 ton ha-1, (T11) 25% RDF + VC@ 10 ton ha-1+, (T12) 25% RDF +  AZ @ 2.5 kg ha-1+FYM @ 10 ton ha-1+VC @ 10 ton ha-1. The treatments were replicated thrice in a Randomized Complete Block Design. The results revealed that Plants treated with 75% RDF + AZ @ 2.5 kg/ha +FYM @ 10 t/ha +VC @ 10 t/ha (T4) significantly recorded maximum vegetative and Tuber yield attributes like plant height (91.87 cm), plant spread (92.38 cm), Number of Branches per plant (7.27), number of leaves per plant (26.53), number of tubers per plant (4.80), maximum tuber weight (958.53) and Maximum tuber yield per plant was recorded in T4 (75% RDF + AZ @ 2.5 kg/ha +FYM @ 10 t/ha +VC @ 10 t/ha) (43.33g) followed by T3 (75% RDF + VC @ 10 t/ha) (40.95g).
该试验于2014-2015年春季在Sam Higginbottom农业技术与科学研究所(前身为阿拉哈巴德农业研究所,AAI-DU)园艺系实验田进行。实验由13治疗即。,(T0) Control-RDF (100:120:100 N P K公斤农业,),(T1) 75% RDF + AZ@ 2.5公斤是,,(T2) RDF +施厩肥@ 10吨是75%,(T3) RDF + VC@ 10吨是75%,(T4) 75% RDF + AZ @ 2.5公斤/公顷农业+ +施厩肥@ 10吨VC @ 10吨农业(T5) RDF + AZ@ 2.5公斤是50%,(T6) RDF + FYM@ 10吨是50%,(T7) RDF + VC@ 10吨是50%,(T8) 50% RDF + AZ @ 2.5公斤是农业+ +施厩肥@ 10吨VC @ 10吨农业(T9) 25% RDF + AZ@ 2.5公斤农业,农业(T10) 25% RDF + FYM@ 10吨,(T11) 25% RDF +VC @ 10吨ha-1+, (T12) 25% RDF + AZ @ 2.5公斤ha-1+FYM @ 10吨ha-1+VC @ 10吨ha-1。在随机完全区组设计中重复了三次治疗。结果表明,75% RDF + AZ @ 2.5 kg/ha +FYM @ 10 t/ha +VC @ 10 t/ha (T4)处理植株的最高营养和块茎产量指标为株高(91.87 cm)、株宽(92.38 cm)、单株分枝数(7.27)、单株叶数(26.53)、单株块茎数(4.80)、单株分枝数(7.27)、单株叶数(26.53)、单株块茎数(4.80)、单株分枝数(7.27)和单株块茎数(4.80)。T4 (75% RDF + AZ @ 2.5 kg/ha +FYM @ 10 t/ha +VC @ 10 t/ha) (43.33g)和T3 (75% RDF +VC @ 10 t/ha) (40.95g)的单株最大块茎重(958.53)和单株最大块茎产量。
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引用次数: 0
Alternative Thermal Processing Technique for Liquid Foods-membrane Processing 液体食品的替代热加工技术——膜加工
Pub Date : 2021-12-13 DOI: 10.9734/ijpss/2021/v33i2430752
S. Shahir
Liquid foods are sensitive to temperature and concentration by conventional methods results in product deterioration. Alternative processes, such as freeze concentration, have the drawback with respect to the maximum achievable concentration (only up to 40 to 45°Brix). In recent years membrane processes such as Microfiltration, Ultrafiltration and reverse osmosis are gaining importance for the concentration of liquid foods. Since heat is not involved in this process, it is also called Alternate thermal processing technique. This process can be employed as a pre-concentration step to reduce water load on subsequent processing steps and can be easily scaled up. Liquid foods such as fruit juices are of high nutritive value as they are naturally enriched with minerals, vitamins and other beneficial components required for human health. When extracted from their sources fruit juices have low solid content, color strength and high-water load. Recent advances and developments in this membrane processing used for the concentration of liquid foods are discussed here.
液体食品对温度和浓度敏感,常规方法会导致产品变质。替代工艺,如冷冻浓缩,在可达到的最大浓度(仅高达40至45°白利度)方面具有缺点。近年来,微滤、超滤和反渗透等膜工艺在液体食品的浓缩中越来越受到重视。由于该过程不涉及热量,因此也称为交替热加工技术。该工艺可以用作预浓缩步骤,以减少后续处理步骤的水负荷,并且可以很容易地扩大规模。果汁等液态食物营养价值很高,因为它们天然富含矿物质、维生素和其他人体健康所需的有益成分。当果汁从它们的来源中提取出来时,果汁的固体含量低,颜色强度高,含水量高。本文讨论了该膜法用于液体食品浓缩的最新进展和发展。
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引用次数: 0
Anti-hyperglycemic Efficiency of the Aqueous Seed Extract of Mucuna pruriens in Nicotinamide-Streptozotocin-induced Diabetic Wistar Rats 麻豆水提取物对烟酰胺-链脲佐菌素诱导的糖尿病大鼠的降糖作用
Pub Date : 2021-12-13 DOI: 10.9734/ijpss/2021/v33i2430751
R. J. I., R. R.
This study was undertaken to evaluate the remedial effect of the aqueous seed extract of Mucuna pruriens (ASEMP) on the endocrine region of pancreas of nicotinamide-streptozotocin-induced diabetes in Wistar rats. The anti-hyperglycemic efficiency of two varieties of Mucuna pruriens var utilis; IIHR Selection 3 and Arka Dhanvantari was investigated. Oral administration of ASEMP against the nicotinamide-streptozotocin-induced diabetes in the Wistar rats showed anti-hyperglycemic effect on the blood glucose level (94 ± 0.2 mg/dl ASEMP 400 mg/kg) when compared with the control group (92 ± 0.2 mg/dl glibenclamide 5 mg/kg), (248 ± 0.3 mg/dl Diabetic control) and (90 ± 0.3 mg/dl Normal control). Significant reduction in creatinine level of the nicotinamide-streptozotocin-induced diabetic rats treated with ASEMP was also recorded. Histopathology examination of the endocrine region of pancreas of the rats revealed restoration of pancreatic islet cells in the diabetic-ASEMP treated rats as the beta cell mass increased and necrotic changes was reduced significantly in contrast with the diabetic control group which showed degenerated pancreatic islet cells. Therefore, this study supports and recommends the exploration of the aqueous seed extract of M. pruriens as an alternative medicine for the management of type-2 diabetes.
本研究探讨了麻豆水提物(ASEMP)对烟酰胺-链脲佐菌素诱导的Wistar大鼠糖尿病胰腺内分泌区的治疗作用。两种麻瓜的抗高血糖作用对IIHR选择3和Arka Dhanvantari进行了研究。与对照组(格列苯脲5 mg/kg)、糖尿病对照组(248±0.3 mg/dl)和正常对照组(90±0.3 mg/dl)相比,口服ASEMP对烟酰胺-链状佐菌素诱导的糖尿病大鼠血糖水平(94±0.2 mg/dl ASEMP 400 mg/kg)有降糖作用。ASEMP对烟酰胺-链脲佐菌素诱导的糖尿病大鼠肌酐水平也有显著降低。胰腺内分泌区组织病理学检查显示,糖尿病- asemp治疗大鼠胰岛细胞恢复,与糖尿病对照组相比,β细胞体积增加,坏死改变明显减少。因此,本研究支持并建议探索花楸水提取物作为治疗2型糖尿病的替代药物。
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引用次数: 0
Variation in Calcium Content of Ginger Germplasm Inoculated with Bio-Agent, Trichoderma viride against Root-Knot Nematode, Meloidogyne incognita 接种绿色木霉防治根结线虫的姜种质钙含量变化研究
Pub Date : 2021-12-13 DOI: 10.9734/ijpss/2021/v33i2430756
Manaswini Mahapatra, D. Nayak, Pranaya Pradhan, A. Mishra
The study was aimed to control nematodes in ginger crop in a sustainable way through inoculation of bio-agent, Trichoderma viride. The outcome of our research revealed that Trichoderma viride alone (T2) was the most effective treatment among all. This bio-agent has lowered the root-knot nematode populations by suppressing the disease intensity and responsible for enhancement of plant growth, increases the availability of nutrients to host plant. Meloidogyne incognita alone (T1) infected rhizomes resulted in highest reduction of calcium content amounting 2.26 and 2.43 mg/100g dry weight whereas maximum content was recorded in Trichoderma viride alone (T2) inoculated rhizomes as 2.84 and 3.08 mg 100g-1 dry weight in suravi and suprabha over control (T7) respectively. Trichoderma viride inoculated 15 days prior to Meloidogyne incognita (TV→MI) was found to be most efficient one among all combined treatments. Susceptibility towards nematode induced more nutrient deposition in rhizomes of ginger crop due to poor translocation process through xylem.
本研究旨在通过接种生物制剂绿木霉(Trichoderma viride)对生姜线虫进行可持续防治。我们的研究结果表明,单独使用绿色木霉(T2)是最有效的治疗方法。该生物制剂通过抑制根结线虫的发病强度,降低了根结线虫的数量,促进了植物的生长,增加了寄主植物的营养利用率。单独侵染黑心木霉(T1)的根状茎中钙含量下降最多,分别为2.26和2.43 mg/100g干重,而单独接种绿木霉(T2)的根状茎中钙含量最高,分别为2.84和3.08 mg/100g干重。以绿木霉(TV→MI)早接种15 d的组合处理效果最好。对线虫的敏感性导致生姜根状茎中养分沉积较多,主要是由于木质部转运过程较差。
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引用次数: 0
Effect of Nutrient Management through Bio Organic Manures on Quality of Acid Lime (Citrus aurantifolia Swingle) 生物有机肥养分管理对酸石灰品质的影响
Pub Date : 2021-12-13 DOI: 10.9734/ijpss/2021/v33i2430754
V. V. Prabha, Avikal Kumar, Y. Angel, S. Abinaya, G. Kumar
Acid lime (Citrus aurantifolia Swingle) is one of the commercial fruit crops which occupies important place in the fruit industry, but yield levels of citrus orchards are still very low. Alternate nutrient management system could help in achieving high yield and quality of acid lime. Thus, an investigation was undertaken on the “Effect of nutrient management through bio-organic manures on quality of acid lime (Citrus aurantifolia Swingle)” in the Department of Horticulture, Faculty of Agriculture, Annamalai University, Annamalainagar, Tamil Nadu, India during 2016 – 2018. The experiment was laid out in randomized block design with twelve treatments, various organic manures, biofertilizers along with inorganic fertilizers including recommended dose of fertilization @ 100%, 75%, 50%, farm yard manure @ 100%, 50%, Vermicompost @ 100%, 50%, Biofertilizers ( 25g Azotobacter + 25g phosphate solubilising bacteria + 150g vesicular arbuscular mycorrhizal) were applied. The quality characters of acid lime were studied at different stages. The observations recorded viz., plant height (m), canopy spread east-west (m), canopy spread north-south (m), canopy height (m), no. of leaves per canopy, leaf area (cm2). The results revealed that plants treated with of T8 -75% RDF + 50% FYM + 50% Vermicompost + Biofertilizers (25g Azotobacter + 25g PSB + 150g VAM) was observed maximum plant height (m), canopy spread east-west (m), canopy spread north-south (m), canopy height (m), no. of leaves per canopy, leaf area (cm2).
酸橙(Citrus aurantifolia Swingle)是我国水果产业中占有重要地位的经济水果作物之一,但我国柑橘果园的产量水平仍然很低。交替养分管理制度有助于提高酸石灰的产量和品质。因此,2016 - 2018年在印度泰米尔纳德邦安纳玛莱大学农学院园林系进行了一项关于“生物有机肥料营养管理对酸石灰(Citrus aurantifolia Swingle)质量的影响”的调查。试验采用随机区组设计,共设12个处理,施用各种有机肥、生物肥料和无机肥料,包括推荐施肥剂量@ 100%、75%、50%,农场厩肥@ 100%、50%,蚯蚓堆肥@ 100%、50%,生物肥料(25g固氮菌+ 25g增磷菌+ 150g囊状菌根)。对酸性石灰在不同阶段的品质特性进行了研究。观测值为:株高(m)、冠层东西向展布(m)、冠层南北向展布(m)、冠层高度(m);每冠层的叶片,叶面积(cm2)。结果表明:T8 -75% RDF + 50% FYM + 50%蚯蚓堆肥+生物肥料(25g固氮菌+ 25g PSB + 150g VAM)处理的植株最高株高(m),冠层东西向展布(m),冠层南北展布(m),冠层高度(m);每冠层的叶片,叶面积(cm2)。
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引用次数: 0
Genome Wide Association Studies to Dissect Genetic Factors Conferring Sheath Blight Resistance in Rice (Oryza sativa L.) 水稻抗纹枯病遗传因子的全基因组关联研究
Pub Date : 2021-12-13 DOI: 10.9734/ijpss/2021/v33i2430755
M. ., K. Ganesamurthy, Sayan Das, R. Saraswathi, C. Gopalakrishnan, R. Gnanam
Rice Sheath blight (ShB) is one of the most serious fungal diseases caused by Rhizoctonia solani. Breeding for sheath blight resistance has been ineffective exercise so far, mainly because of lack of good number of reliable sources of resistance in rice germplasm. In this context our studies indicated that the lines Tetep, Jasmine 85 and MTU 9992 confer resistant to moderately resistant reaction against the pathogen. The current investigation was carried out to dissect the genetic factors governing resistance to sheath blight through genome wide association study (GWAS) from the mapping populations developed by design where in, each of the resistant parents were crossed to three to four highly susceptible parents to generate eleven populations (Jasmine 85XTN1, Jasmine 85XSwarnaSub1, Jasmine 85XII32B, Jasmine 85XIR54, TetepXTN1, TetepXSwarnaSub1, TetepXII32B, TetepXIR54, MTU 9992XTN1, MTU 9992XII32B and MTU 9992XIRBB4). A total of 1545 Recombinant inbred lines (RILs) derived from eleven crosses were used for the study. During rainy 2020 the F7 RILs were screened for their reaction to Sheath blight in two hot spot locations. The genotyping was done with Illumina platform having 6564 SNP markers. Genome wide association study was done with two models Generalized Linear Model (GLM) and Mixed Linear Model (MLM). Results clearly indicate the superiority of MLM over GLM in correcting the population structure. With MLM model, in Jasmine 85 half-sib populations with 565 RILs analyzed, five QTLs (Quantitative Trait Loci) were detected on Chr1, Chr3, Chr9, Chr10 and Chr11 with –log10 (P-Value) more than 3. In TETEP half-sib populations with 714 RILs examined, seven QTLs were observed on Chr1, Chr2, Chr5, Chr6, Chr7, Chr8, and Chr11 with –log10 (P-Value) more than 4. Whereas in MTU 9992 half-sib populations with 266 RILs studied, three novel QTLs were identified on Chr2, Chr6 and Chr11 with –log10 (P-Value) more than 3. Some of these QTLs were reported by researches earlier. In the current research, some novel QTLs were detected in Jasmine 85 (Chr10) and Tetep (Chr2, Chr5 and Chr6) apart from three new QTLs discovered in MTU 9992. The results facilitated to have better understanding of the genetic basis for sheath blight resistance in rice. Pyramiding all the QTL identified so far into a susceptible varieties is complicated affair as resistance is governed by not only several large effect QTLs but also medium to small effect QTLs as well, hence genomic selection approach could be rewarding for breeding for sheath blight resistance.
水稻纹枯病(ShB)是由枯丝核菌引起的最严重的真菌病害之一。水稻抗纹枯病育种至今成效不佳,主要原因是水稻种质资源中缺乏大量可靠的抗性来源。在这种情况下,我们的研究表明,testep, Jasmine 85和MTU 9992系对病原体具有中等抗性反应。本研究通过基因组全关联研究(GWAS)从设计开发的定位群体中分析控制鞘枯病抗性的遗传因素,其中每个抗性亲本与3至4个高易感亲本杂交,产生11个群体(Jasmine 85XTN1, Jasmine 85XSwarnaSub1, Jasmine 85XII32B, Jasmine 85XIR54, TetepXTN1, TetepXSwarnaSub1, TetepXII32B, TetepXIR54, MTU 9992XTN1,MTU 9992XII32B和MTU 9992XIRBB4)。利用11个杂交组合的1545个重组自交系(RILs)进行研究。在多雨的2020年,在两个热点地区筛选了F7 ril对鞘疫病的反应。基因分型采用具有6564个SNP标记的Illumina平台。采用广义线性模型(GLM)和混合线性模型(MLM)进行全基因组关联研究。结果清楚地表明,MLM在校正种群结构方面优于GLM。利用MLM模型,在茉莉85个半同胞群体中,共分析了565个ril,在Chr1、Chr3、Chr9、Chr10和Chr11上检测到5个qtl(数量性状位点),其-log10 (p值)大于3。在共有714个RILs的TETEP半同胞群体中,在Chr1、Chr2、Chr5、Chr6、Chr7、Chr8和Chr11上共观察到7个qtl,且-log10 (p值)大于4。在MTU 9992同母同胞群体中,共鉴定出266个rls,在Chr2、Chr6和Chr11上鉴定出3个新的qtl, p值大于3。其中一些qtl早前已被报道。在目前的研究中,除了在MTU 9992中发现3个新的qtl外,还在Jasmine 85 (Chr10)和Tetep (Chr2, Chr5和Chr6)中发现了一些新的qtl。研究结果有助于更好地了解水稻抗白叶枯病的遗传基础。由于抗性不仅由几个大效QTL控制,而且由中效到小效QTL控制,因此基因组选择方法可能对抗鞘疫病育种有益,因此将迄今已鉴定的所有QTL金字塔化到一个易感品种是一件复杂的事情。
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引用次数: 0
Using Zeolite and Vermicompost Amendments to Improve Water Productivity of Wheat Irrigated by Low-quality Water in the Northern Nile Delta 利用沸石和蚯蚓堆肥改良剂提高北尼罗河三角洲低质水灌溉小麦的水分生产力
Pub Date : 2021-12-13 DOI: 10.9734/ijpss/2021/v33i2430760
T. Khalifa, M. Ramadan, Mona S. M. Eid
Aims: In the long run, reusing low-quality water in Egypt's agricultural sector directly or after mixing with fresh water to compensate for water supply constraints can be hazardous to plants and soil. As a result, some appropriate management must be considered. For this reason, a field experiment was implemented in winter seasons 2018/2019 and 2019/2020 at Sakha Agric. Res. Station Farm, Kafr El-Sheikh Gov., Egypt. This study aims to assess the impacts of zeolite and vermicompost as well their combinations on alleviation of low-quality water impacts on physicochemical properties of clayey soil and wheat productivity. Study Design:  complete randomized block design with three replicates. Results: The application of 2.40 Mg Z ha-1 was found to be the most effective on soil properties and plant growth. This treatment reduced soil EC, Na+, Ca2+, Mg2+, Cl-, SO42-, and ESP values the most (52.90 percent, 83.21 percent, 30.43 percent, 6.04 percent, 91.82 percent, 19.83 percent, and 70.73 percent, respectively), while increasing the K+ value by 32.47 percent. It also achieved the highest increases in plant height, 1000-grain weight, grain, and straw yields (35.92%, 9.60%, 42.77%, and 25.61%, respectively) when compared to untreated soil. With 2.40 Mg VC ha-1, the greatest changes in bulk density, total porosity, and CEC (-9.23, 9.30, and 10.54 percent, respectively) were obtained. The applications of 1.80 Mg Z with 0.6 Mg VC ha-1 and 0.6 Mg Z ha-1 with 1.80 Mg VC ha-1, on the other hand, resulted in the greatest increases in soil moisture content, drainable pores (DP), and water holding pores (WHP). Furthermore, 0.6 Mg Z combined with 1.80 Mg VC ha-1 significantly increased the available N, P, and K in the soil. The addition of 2.4 tons Z/ha increased the WP and resulted in a high economically appealing wheat. Conclusion: It could be concluded that the application of Z and VC is a new strategy for alleviating abiotic stress and improving wheat growth. Z application was more effective than VC on improving soil physicochemical properties and improving the water productivity and achieve high economical attractiveness wheat irrigated by low-quality water.
目的:从长远来看,在埃及的农业部门直接或混合淡水后再利用低质量的水来弥补供水限制可能对植物和土壤有害。因此,必须考虑适当的管理。为此,Sakha农业公司于2018/2019和2019/2020冬季进行了一项田间试验。埃及Kafr El-Sheikh Gov的Res. Station农场。本研究旨在评估沸石和蚯蚓堆肥及其组合对缓解低质水对粘土土壤理化性质和小麦生产力的影响。研究设计:完全随机区组设计,有3个重复。结果:施用2.40 Mg zha -1对土壤性质和植物生长最有效。该处理降低土壤EC、Na+、Ca2+、Mg2+、Cl-、SO42-和ESP值最多(分别为52.90%、83.21%、30.43%、6.04%、91.82%、19.83%和70.73%),K+值增加32.47%。在株高、千粒重、籽粒和秸秆产量方面,与未处理土壤相比,分别提高了35.92%、9.60%、42.77%和25.61%。当VC ha-1含量为2.40 Mg时,堆密度、总孔隙度和CEC的变化最大(分别为- 9.23%、9.30%和10.54%)。1.80 Mg Z + 0.6 Mg VC ha-1和0.6 Mg Z ha-1 + 1.80 Mg VC ha-1处理土壤含水量、疏水孔(DP)和持水孔(WHP)增加幅度最大。此外,0.6 Mg Z配以1.80 Mg VC ha-1显著提高了土壤速效氮、磷和钾。添加2.4吨氮/公顷提高了小麦的水分利用率,从而获得了具有较高经济吸引力的小麦。结论:应用Z和VC是缓解非生物胁迫、促进小麦生长的新策略。施用Z比VC更有效地改善土壤理化性质和提高水分生产力,实现低质水灌溉小麦的高经济吸引力。
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引用次数: 2
期刊
International Journal of Plant & Soil Science
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