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Microbiological integration for qualitative improvement of vermicompost 微生物整合对蚯蚓堆肥的定性改进
IF 1.5 Q1 Agricultural and Biological Sciences Pub Date : 2021-03-25 DOI: 10.30486/IJROWA.2021.1902019.1087
S.V.Vi mal, G. Chattopadhyay, K. Chakrabarti
Purpose This study was carried out to assess the effect of integrating microbiological fortification with vermicomposting process on biofertilizing quality of vermicompost as well as improvement of the availability of nitrogen and phosphorus in the product. Method A mixture of cow dung and vegetable market waste (1:1) was used for vermicomposting with Eisenia foetida as the decomposer earthworm @ 10 no.of worm kg-1 substrate. Nitrogen fixing and phosphate solubilizing bacteria (NFB and PSB)Azotobacter chroococcum and Pseudomonas fluorescens were integrated with this composting process at varying  doses viz. 0,5 and 10 g kg-1 substrate under different combinations. Changes in the population of these two bio-fertilizing microorganisms and the availability of relevant nutrients in the substrates were monitored periodically to assess the behaviors of these microorganisms and their effects on the produced vermicompost. Results All the inoculations resulted in substantial increments in population of both NFB and PSB over the control. However, the increments were more prominent for NFB than the PSB. Significant increments in the amount of mineralized nitrogen and solubilized phosphate over the control were observed in almost all the treatment combinations. Conclusion Integration of nitrogen fixing and phosphate solubilizing bacteria with vermicomposting process resulted in substantial enrichment of the product. This benefit was observed not only in terms of increased availability of the two major plant nutrients of concern viz. N and P, but also in significant improvement in the population of the inoculated microorganisms turning the product into a potential source of bio-fertilizers.
目的评价微生物强化与蚯蚓堆肥工艺相结合对蚯蚓堆肥生物肥料品质及提高产品氮磷有效性的影响。方法采用牛粪与菜市场垃圾按1:1比例混合进行蚯蚓堆肥,腐熟爱森虫为分解者,蚯蚓@ 10号。蠕虫kg-1基质。固氮和增磷细菌(NFB和PSB)、绿球菌固氮细菌和荧光假单胞菌在不同组合下以不同剂量(0、5和10 g kg-1底物)加入堆肥过程。定期监测这两种生物施肥微生物的种群变化和基质中相关营养物质的有效性,以评估这些微生物的行为及其对产生的蚯蚓堆肥的影响。结果所有接种均导致NFB和PSB的种群数量较对照组显著增加。然而,与PSB相比,NFB的增量更为显著。在几乎所有的处理组合中,矿化氮和溶解磷酸盐的量都比对照显著增加。结论固氮溶磷菌与蚯蚓堆肥工艺相结合,可使产物大量富集。这种益处不仅体现在两种主要植物营养物质(氮和磷)的可用性增加,而且还体现在接种微生物种群的显著改善,使产品成为生物肥料的潜在来源。
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引用次数: 0
Investigating the effects of cow manure, vermicompost and Azolla fertilizers on hydraulic properties of saline-sodic soils 研究了牛粪、蚯蚓堆肥和氮肥对盐碱土水力学特性的影响
IF 1.5 Q1 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.30486/IJROWA.2020.1893432.1039
Parastoo Sharifi, M. Shorafa, M. Mohammadi
Purpose Soil salinity and sodicity are among the factors involved in soil degradation, especially in arid and semi-arid areas of the world. The use of modifiers, including organic matters, can be considered as an appropriate strategy to improve the fertility of saline-sodic soils.  Method In this study, saline-sodic control soil was collected from Karaj and mixed with three levels of 1%, 3% and 5% of cow manure, vermicompost and Azolla. The soil columns were then incubated at 20 °C and field capacity moisture for 5 months. The physical and chemical properties of the treatments were evaluated before and after incubation. Results After the incubation period, the lowest salinity level was observed in 5% Azolla and vermicompost treatments. The highest amount of change in sodium absorption ratio was related to 5% cow manure treatment. There was an insignificant difference in moisture levels in a given suction among the different treatments. After the incubation period, salinity and sodium absorption decreased and increased in most of the treatments, respectively. Moreover, the decrease of saturation dehydration coefficient in the treatments revealed the disruption of soil structure and conversion of large pores to fine grains as a result of adding the mentioned organic matters. Conclusion According to the results, cow manure at 1% level had no significant effect on soil properties. However, at higher levels, it had a negative effect on quality and conditions of the saline-sodic soil in terms of physical and chemical properties. In contrast, Azolla and vermicompost fertilizers at 5% proved to be suitable for correcting the saline-sodic soil.
土壤盐分和碱度是导致土壤退化的因素之一,特别是在世界干旱和半干旱地区。使用改良剂,包括有机质,可以被认为是提高盐碱地肥力的适当策略。方法在卡拉伊采盐碱化对照土,分别添加1%、3%和5%的牛粪、蚯蚓堆肥和杜鹃花3个水平。然后将土柱在20°C和田间水分条件下孵育5个月。对培养前后处理的理化性质进行了评价。结果孵育期结束后,5%杜鹃和蚯蚓堆肥处理的含盐量最低。在5%牛粪处理下,钠吸收比变化最大。在不同处理之间,同一吸力下的水分水平差异不显著。孵育期结束后,大部分处理的盐度和钠吸收分别下降和增加。饱和脱水系数的降低反映了有机质对土壤结构的破坏和大孔隙向细颗粒的转化。结论1%水平的牛粪对土壤性质无显著影响。然而,在较高水平上,它在物理和化学性质方面对盐碱土的质量和条件产生了负面影响。相比之下,5%的氮肥和蚯蚓堆肥对盐碱化土壤的修复效果较好。
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引用次数: 2
Sawdust as an ameliorant to decontaminate Arsenic in potato soils 木屑对马铃薯土壤砷的改良净化作用
IF 1.5 Q1 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.30486/IJROWA.2021.1899077.1068
Md. Nousad Hossain, T. Roy, M. Mostofa
Purpose Bangladesh is an agriculture-based country, where about 3 million ha is covered by groundwater sources for irrigation, but a significant portion of irrigation water is Arsenic contaminated. To produce Potato under Arsenic contaminated soil, an experiment was conducted to find out the effect of sawdust as an adsorbent to decontaminate Arsenic toxicity in soil. Method The research was conducted in pot experiment. It consisted of two factors. Factor A: Arsenic levels (4) viz., As0: control (0 mg/kgsoil), As1: 25 mg/kg soil, As2: 50 mg/kg soil, and As3: 75 mg/kg soil. Factor B: Sawdust levels (4) viz., S0: control (0 g/kg soil), S1: 10 g/kg soil, S2: 50 g/kg soil, and S3: 100 g/kg soil. Results Arsenic content in Potato tuber peel and flesh gradually increased with the increase of Arsenic levels. As3 was found for the highest accumulation of Arsenic in tuber peel (3.867 mg/kgfresh weight) and flesh (0.6236 mg/kgfresh weight). Arsenic content in both peel and flesh of Potato tuber decreased with increasing sawdust levels. The soil treated with S3 reduced 86.41% and 51.44% Arsenic accumulation from tuber peel and flesh, respectively, compared to control (S0). Potato produced from the treatment As1S1 accumulated a lower amount of Arsenic (0.15 mg/kgfresh weight) in Potato flesh compared to those of other treatments. Conclusion Therefore, Potato growers can produce Potato in 25 mg/kg Arsenic contaminated soil treated with 10 g sawdust/kg soil, which contains a minimum than the critical level of Arsenic for human consumption.
孟加拉国是一个以农业为基础的国家,大约有300万公顷的地下水用于灌溉,但很大一部分灌溉用水受到砷污染。为了在砷污染土壤下生产马铃薯,研究了木屑作为吸附剂对土壤砷毒性的去除效果。方法采用盆栽试验方法进行研究。它由两个因素组成。因子A:砷水平(4),即As0:对照(0 mg/kg土壤),As1: 25 mg/kg土壤,As2: 50 mg/kg土壤,As3: 75 mg/kg土壤。因素B:锯末水平(4),即,S0:对照(0克/公斤土壤),S1: 10克/公斤土壤,S2: 50克/公斤土壤,S3: 100克/公斤土壤。结果马铃薯块茎皮和果肉中砷含量随砷含量的增加而逐渐升高。As3在块茎皮(3.867 mg/kg鲜重)和果肉(0.6236 mg/kg鲜重)中砷积累量最高。马铃薯块茎中砷含量随木屑浓度的增加而降低。与对照(S0)相比,S3处理的块茎皮和块茎肉中砷的累积量分别减少了86.41%和51.44%。与其他处理相比,As1S1处理生产的马铃薯在马铃薯果肉中积累的砷含量较低(0.15 mg/kg鲜重)。结论马铃薯种植户可在含砷量为25 mg/kg、含砷量为10 g/kg的木屑土壤中生产马铃薯。
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引用次数: 0
Soil aggregation indexes and chemical and physical attributes of aggregates in a Typic Hapludult fertilized with swine manure and mineral fertilizer 猪粪与矿肥混合施用典型单倍体土壤团聚体指标及团聚体理化性质
IF 1.5 Q1 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.30486/IJROWA.2021.1896960.1051
Guilherme Wilbert Ferreira, Lucas Benedet, T. Trapp, A. P. Lima, V. M. Junior, A. Loss, C. R. Lourenzi, J. J. Comin
Purpose The objective was to evaluate the effects of mineral fertilizers and swine manure fertilization on soil aggregation indexes and on chemical and physical attributes of aggregates in Typic Hapludult managed under minimum tillage system. Method Experiment was implemented in 2013, in southern Brazil. The treatments were control, mineral fertilization (MF), swine manure compost + MF (SMC+MF); pig slurry + MF (PS+MF); PS to supply N for maize and black oat (PS100). In May 2015 and 2016, undisturbed soil samples were collected to obtain soil aggregates. Aggregates stability was evaluated through the mean weight diameter (MWD) and mean geometric diameter (MGD) indexes and aggregate distribution by diameter classes. In macroaggregates, total organic carbon (TOC), total nitrogen (TN), clay flocculation degree (CF%) and ∆pH were evaluated. Results The use of swine manure, associated or not to MF, increases TOC and TN contents in soil aggregates in 67.02 and 125.87%, respectively, for SMC+MF treatment. However, it was not efficient in improving soil physical attributes, reducing soil aggregation indexes, mainly in the 5-10 cm layer, by the decreased values of MWD and MGD. This result corroborates with the increase in microaggregates in all treatments. This was a result of the negative ∆pH values and the increased CF%. Conclusion Despite the increase in organic matter contents observed in this study, this was not enough to guarantee an improvement in soil physical attributes over 4 years. These results show that management must be supported by several conservationist techniques in order to have soil quality.
目的评价施用矿肥和猪粪对典型单倍体土壤团聚体指标及团聚体理化性状的影响。方法实验于2013年在巴西南部实施。处理为对照、矿物肥(MF)、猪粪堆肥+MF (SMC+MF);猪浆+MF (PS+MF);PS为玉米和黑燕麦提供氮素(PS100)。2015年5月和2016年5月采集原状土样,获取土壤团聚体。通过平均重量直径(MWD)、平均几何直径(MGD)指标和粒径级分布对团聚体稳定性进行评价。测定大团聚体中总有机碳(TOC)、总氮(TN)、粘土絮凝度(CF%)和pH值。结果猪粪处理对土壤团聚体TOC和TN含量的影响,无论是否与有机肥相关,分别为67.2%和125.87%。然而,通过降低MWD和MGD值,对改善土壤物理属性、降低土壤团聚指数(主要在5 ~ 10 cm层)没有效果。这一结果证实了所有处理中微聚集体的增加。这是负的∆pH值和增加的CF%的结果。结论尽管本研究中观察到有机质含量的增加,但这并不足以保证4年内土壤物理属性的改善。这些结果表明,为了保持土壤质量,管理必须辅以几种保护技术。
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引用次数: 3
Evaluation of the sensitivity and production of flowers in Tagetes erecta L. exposed to high doses of sodium from irrigation with landfill leachates 垃圾渗滤液灌溉高剂量钠对万寿菊(Tagetes erecta L.)花的敏感性及产量评价
IF 1.5 Q1 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.30486/IJROWA.2021.1900504.1078
M. Abrile, M. L. Fiasconaro, S. Gervasio, M. Antolín, M. Lovato
Purpose Reuse of landfill leachate is an effective alternative for their nutrients to mitigate decrease in freshwater. On the other hand, the growth of vegetation in the final disposal areas provides many benefits such as improving the visual impact, controlling of hydric erosion, etc. The purpose of this work was to evaluate landfill leachate as irrigation water and source of nutrients for growth of Tagetes erecta L., an ornamental plant with phytoremediation capacities. Method Vegetal growth, physiological responses and mineral elements uptake of the ornamental plant Tagetes erecta L. were studied with different levels of landfill leachate irrigation. The landfill leachate was provided by the municipal waste treatment. Experimental period covered 34 days of daily watering between the beginning and end of the flowering stage. Three different irrigation treatments were used: T1: 10% leachate; T2: 25% leachate; T3: 50% leachate. Hoagland's solution served as the control treatment. Results The irrigation treatment with a dose of 50% leachate causes a clear deterioration in the plant and its flowers. In lower doses, the plant responds favorably to both the production of flowers and the main features of them. Also, the water-use efficiency (WUE) is diminished in those plants irrigated with the major dose of leachate. Conclusion Maintaining controlled doses, landfill leachates can be used as an alternative source of water and nutrients. Irrigation with leachates of these characteristics should be done in dosages not higher than 25% to avoid possible damage of Tagetes erecta L. growth.
目的垃圾渗滤液的再利用是一种有效的替代其营养物质的方法,以缓解淡水的减少。另一方面,最终处置区植被的生长提供了许多好处,如改善视觉影响,控制水蚀等。研究了垃圾渗滤液作为具有植物修复能力的观赏植物万寿菊(Tagetes erecta L.)生长的灌溉用水和养分来源。方法研究不同渗滤液灌溉水平下观赏植物万寿菊的生长、生理反应及对矿质元素的吸收。垃圾渗滤液由城市垃圾处理提供。试验期从开花开始到开花结束,每天浇水34 d。采用三种不同的灌溉处理:T1: 10%渗滤液;T2: 25%渗滤液;T3: 50%渗滤液。霍格兰溶液作为对照处理。结果用50%渗滤液灌溉后,植株和花明显变质。在较低剂量下,植物对花的产生和花的主要特征都有良好的反应。同时,大剂量渗滤液灌溉的植物水分利用效率降低。结论在控制剂量的情况下,垃圾渗滤液可作为水体和营养物的替代来源。用这些特性的渗滤液灌溉时,用量不应超过25%,以免对万寿菊生长造成损害。
{"title":"Evaluation of the sensitivity and production of flowers in Tagetes erecta L. exposed to high doses of sodium from irrigation with landfill leachates","authors":"M. Abrile, M. L. Fiasconaro, S. Gervasio, M. Antolín, M. Lovato","doi":"10.30486/IJROWA.2021.1900504.1078","DOIUrl":"https://doi.org/10.30486/IJROWA.2021.1900504.1078","url":null,"abstract":"Purpose Reuse of landfill leachate is an effective alternative for their nutrients to mitigate decrease in freshwater. On the other hand, the growth of vegetation in the final disposal areas provides many benefits such as improving the visual impact, controlling of hydric erosion, etc. The purpose of this work was to evaluate landfill leachate as irrigation water and source of nutrients for growth of Tagetes erecta L., an ornamental plant with phytoremediation capacities. Method Vegetal growth, physiological responses and mineral elements uptake of the ornamental plant Tagetes erecta L. were studied with different levels of landfill leachate irrigation. The landfill leachate was provided by the municipal waste treatment. Experimental period covered 34 days of daily watering between the beginning and end of the flowering stage. Three different irrigation treatments were used: T1: 10% leachate; T2: 25% leachate; T3: 50% leachate. Hoagland's solution served as the control treatment. Results The irrigation treatment with a dose of 50% leachate causes a clear deterioration in the plant and its flowers. In lower doses, the plant responds favorably to both the production of flowers and the main features of them. Also, the water-use efficiency (WUE) is diminished in those plants irrigated with the major dose of leachate. Conclusion Maintaining controlled doses, landfill leachates can be used as an alternative source of water and nutrients. Irrigation with leachates of these characteristics should be done in dosages not higher than 25% to avoid possible damage of Tagetes erecta L. growth.","PeriodicalId":14373,"journal":{"name":"International Journal Of Recycling of Organic Waste in Agriculture","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90634222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Solubility and physical composition of rice husk ash silica as a function of calcination temperature and duration 稻壳灰二氧化硅的溶解度和物理组成随煅烧温度和时间的变化
IF 1.5 Q1 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.30486/IJROWA.2021.1899156.1069
Ryoko Sekifuji, V. C. Le, M. Tateda, H. Takimoto
Purpose The solubility of silica is a key parameter affecting its suitability as a rice fertilizer. Therefore, this study determined the effect of calcination temperature and duration on the physical composition of silica derived from rice husk ash. Method Rice husks were calcined at 100–900 °C for 15–120 min in an electric furnace. The solubility and physical composition of the samples were measured, and the appearance of the calcined rice husk ash was determined via visual observations. Results The appearance of the rice husk ash changed drastically at a calcination temperature of 300 °C. The husk exhibited the whitest color at 500 °C and 120 min of calcination. The solubility of silica in the rice husk ash increased up to a calcination temperature of 500 °C and then started to decrease. The silica in the rice husk ash exhibited a gray zone between the amorphous and crystalline structures. Conclusion For actual field applications of silica derived from rice husk ash as a fertilizer, calcination conditions of 400–800 °C and 15 min are proposed to improve solubility. The optimal calcination temperature should be determined based on the heat recovery efficiency.
目的二氧化硅的溶解度是影响其作为水稻肥料适用性的关键参数。因此,本研究确定了煅烧温度和煅烧时间对稻壳灰制备的二氧化硅物理组成的影响。方法稻壳在电炉中于100-900℃下煅烧15-120 min。测定了样品的溶解度和物理组成,并通过目测测定了煅烧稻壳灰的外观。结果在300℃的煅烧温度下,稻壳灰分的外观发生了很大的变化。在500℃、120 min煅烧时,稻壳呈现出最白的颜色。稻壳灰中二氧化硅的溶解度在煅烧温度为500℃时呈上升趋势,随后开始下降。稻壳灰中的二氧化硅呈现出介于无定形和结晶结构之间的灰色地带。结论稻壳灰二氧化硅作为肥料在实际应用中,建议在400 ~ 800℃、15 min的煅烧条件下提高其溶解度。最佳煅烧温度应根据热回收效率确定。
{"title":"Solubility and physical composition of rice husk ash silica as a function of calcination temperature and duration","authors":"Ryoko Sekifuji, V. C. Le, M. Tateda, H. Takimoto","doi":"10.30486/IJROWA.2021.1899156.1069","DOIUrl":"https://doi.org/10.30486/IJROWA.2021.1899156.1069","url":null,"abstract":"Purpose The solubility of silica is a key parameter affecting its suitability as a rice fertilizer. Therefore, this study determined the effect of calcination temperature and duration on the physical composition of silica derived from rice husk ash. Method Rice husks were calcined at 100–900 °C for 15–120 min in an electric furnace. The solubility and physical composition of the samples were measured, and the appearance of the calcined rice husk ash was determined via visual observations. Results The appearance of the rice husk ash changed drastically at a calcination temperature of 300 °C. The husk exhibited the whitest color at 500 °C and 120 min of calcination. The solubility of silica in the rice husk ash increased up to a calcination temperature of 500 °C and then started to decrease. The silica in the rice husk ash exhibited a gray zone between the amorphous and crystalline structures. Conclusion For actual field applications of silica derived from rice husk ash as a fertilizer, calcination conditions of 400–800 °C and 15 min are proposed to improve solubility. The optimal calcination temperature should be determined based on the heat recovery efficiency.","PeriodicalId":14373,"journal":{"name":"International Journal Of Recycling of Organic Waste in Agriculture","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90823947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Detoxification of soybean oil mill effluent using anaerobic digestion and the suitability of the digestate for fertigation 厌氧消化对豆油厂废水的解毒及厌氧消化液的适宜性
IF 1.5 Q1 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.30486/IJROWA.2021.1896893.1050
Ezinne E. Okorie, A. Ibrahim
Purpose The upsurge of vegetable oil production in Nigeria and the equally increasing concern for the environment which arises due to indiscriminate disposal of phytotoxic vegetable oil mill effluent in a less regulated country like Nigeria makes the re-use of the effluent for fertigation a suitable strategy for its disposal if the appropriate treatment technique is adopted. Method  A laboratory experiment was set up to study how the detoxification of soybean oil mill effluent (SOME) under different anaerobic digestion time (0, 20 and 40 days) and application rates (0, 50 and 100 m3/ha) will influence the suitability of the effluent for fertigation. Results SOME has a pH of 8.3, which decreased steadily to 7.4 as digestion time increased to 40 days. EC and potassium levels also decreased as digestion time increased, from 0.37 dS/m and 120.6 g/l to 0.28 dS/m and 70.1 g/l, respectively. However, levels of organic carbon, nitrogen and phosphorus did not change significantly. The effluent impacted negatively on germination after a one-time application, with the germination index going as low as 22% for untreated SOME and gradually increasing to 66% as digestion time increased. Continuous application of the untreated effluent also affected soil microbial activity negatively when compared to the treated effluent. Conclusion Anaerobic digestion detoxifies SOME and the efficiency of the treatment increased with increasing digestion time. The effluent also contains low to moderate amounts of NPK and therefore has a potential for fertigation.
尼日利亚植物油生产的激增以及对环境的同样日益关注,这是由于在尼日利亚这样一个管制较少的国家不分青红皂白地处置植物毒性植物油厂废水而引起的,因此,如果采用适当的处理技术,将废水重新用于施肥是一项适当的处理战略。方法通过室内试验,研究了不同厌氧消化时间(0、20、40 d)和施用量(0、50、100 m3/ha)对大豆油厂出水解毒效果的影响。结果SOME的pH值为8.3,随着消化时间增加至40 d, pH稳定下降至7.4。EC和钾含量也随消化时间的延长而降低,分别从0.37 dS/m和120.6 g/l降至0.28 dS/m和70.1 g/l。然而,有机碳、氮和磷的水平没有显著变化。一次性施用后,污水对发芽产生负面影响,未经处理的发芽指数低至22%,随着消化时间的增加,萌发指数逐渐上升至66%。与处理过的污水相比,连续施用未经处理的污水也会对土壤微生物活性产生负面影响。结论厌氧消化对SOME的解毒作用随着消化时间的延长而增强。废水还含有低至中等量的氮磷钾,因此具有施肥潜力。
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引用次数: 0
Valorization of vermicompost with bacterial fermented chicken feather hydrolysate for the yield improvement of tomato plant: A novel organic combination 细菌发酵鸡毛水解液与蚯蚓堆肥复合提高番茄产量:一种新的有机组合
IF 1.5 Q1 Agricultural and Biological Sciences Pub Date : 2021-03-01 DOI: 10.30486/IJROWA.2020.1904599.1104
Ishita Biswas, Debasis Mitra, A. Senapati, D. Mitra, Sourav Chattaraj, Mursheda K. Ali, G. Basak, P. Panneerselvam, P. Mohapatra
Purpose Chicken feather protein hydrolysate (CFPH) has drawn a significant attention as a component/type of biofertilizer in recent years, because of the beneficial impact on the growth of the plant. The current study aims to evaluate the potential influence of the combination of CFPH with vermicompost (VC) on growth-promotion and yield improvement in tomato plants. Method Feather degrading bacteria were isolated and characterized using 16s-rDNA sequencing, and assessed for biochemical reactions, growth-promoting attributes and keratinase activity. The medium used for feather degradation studies consisted of 0.75% (w/v) of raw feather, with 1% (v/v) of inoculum at 37°C, pH 7.5 and at 120 rpm. A field study was done by randomized block design (RBD) with five treatments in tomato. Results Keratinolytic and feather degrading bacteria isolated and used in this study were identified as Bacillus cereus PKID1 with accession number MT158702. The bacterium gave the highest keratinase activity of 80±0.28 U/ml. The CFPH showed the potential to promote remarkably the germination % of tomato (84.13), rice (87.24), onion (84.13), chilli (84.13), chickpea (73.24) seeds ; field experiment significantly increased plant growth and yield compared with control. Conclusion The principal component analysis of the field experiment as a result of tomato plant-growth, the order of best treatment efficacy for improvement of parameter estimates was as follows: CFPH and VC > CFPH > VC > recommended dose of fertilizers (RDF) > control. Thus, the application of CFPH with VC could improve the productivity of crops and decrease the use of chemical fertilizers.
鸡毛蛋白水解物(CFPH)因其对植物生长的有益影响,近年来作为一种生物肥料备受关注。本研究旨在评价CFPH与蚯蚓堆肥(VC)联合施用对番茄植株生长和增产的潜在影响。方法分离羽毛降解菌,采用16s-rDNA测序对其进行鉴定,并对其生化反应、促生长特性和角化酶活性进行评价。用于羽毛降解研究的培养基为0.75% (w/v)的生羽毛和1% (v/v)的接种量,温度为37℃,pH为7.5,转速为120 rpm。采用随机区组设计(RBD)对番茄进行了5个处理的田间试验。结果本研究分离到的角质分解和羽毛降解细菌鉴定为蜡样芽孢杆菌PKID1,登录号为MT158702。该细菌的角化酶活性最高,为80±0.28 U/ml。CFPH对番茄(84.13)、水稻(87.24)、洋葱(84.13)、辣椒(84.13)、鹰嘴豆(73.24)种子的发芽率有显著的促进作用;田间试验与对照相比,显著提高了植株的生长和产量。结论田间试验主成分分析结果表明,对番茄植株生长状况进行改善的最佳处理效果参数估计顺序为:CFPH和VC > CFPH > VC >推荐用量肥料(RDF) >控制。综上所述,CFPH与VC配合施用可以提高作物生产力,减少化肥用量。
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引用次数: 9
Innovations in market crop waste compost production: Use of black soldier fly larvae and biochar 市场作物废弃物堆肥生产的创新:使用黑虻幼虫和生物炭
IF 1.5 Q1 Agricultural and Biological Sciences Pub Date : 2021-02-22 DOI: 10.30486/IJROWA.2021.1899111.1071
A. M. Akumah, E. Nartey, G. K. Ofosu-Budu, E. Ewusie, B. Offei, N. Adamtey
Purpose Compost production technology and use are known among Ghanaians farmers. However, the long composting period averaging three months has had adverse effect on adoption. The black soldier fly (Hermetia illucens) larvae (BSFL) feed voraciously and breaks down organic wastes fast. Addition of rice husk biochar (RHB) neutralises acidity and aerates the compost. Combined application of BSFL and RHB to feedstocks could therefore reduce composting period and improve compost quality. Method Two studies were conducted with market crop waste (MCW) as feedstock. Firstly, feedstocks at two particle sizes (x 10 mm), with and without BSFL were evaluated to determine degradability, chemical content of the degraded residue and to select appropriate feedstock size ideal for composting. Secondly, the selected feedstocks size of between 5 mm and 10 mm were inoculated with or without BSFL and RHB of 0%, 5% 10% and 15% added. Composting trials were conducted in barrels inclined at 30o to facilitate drainage. Physicochemical and biological parameters of feedstock were monitored until maturity. Results Degradability of MCW by BSFL was feedstock specific rather than feedstock size. Inoculation of BSFL and biochar addition reduced composting period from 76 to 45 days. Biochar addition at 15% increased P availability to 1882 mg kg-1 but reduced total N to 10.5 g kg-1. E coli levels decreased in the BSFL-biochar composts to acceptable limits. Conclusion Composting MCW with BSFL and biochar reduced composting period and improved compost quality.
目的堆肥生产技术和使用在加纳农民中是众所周知的。然而,平均三个月的长堆肥期对采用产生了不利影响。黑兵蝇(Hermetia illucens)幼虫(BSFL)食性强,能快速分解有机废物。添加稻壳生物炭(RHB)可以中和酸度并使堆肥透气。因此,BSFL和RHB在原料中联合施用可以缩短堆肥周期,提高堆肥质量。方法以市售作物废弃物为原料进行两项研究。首先,对两种粒径(x 10 mm)的原料(有和没有BSFL)进行了评估,以确定降解残渣的可降解性、化学成分,并选择适合堆肥的合适原料粒度。其次,选取粒径为5 ~ 10 mm的原料,分别以0%、5%、10%和15%的添加量分别接种BSFL和RHB。堆肥试验在倾斜300度的桶中进行,以方便排水。对原料的理化和生物学参数进行监测,直至原料成熟。结果BSFL对MCW的降解能力与原料粒度无关。接种BSFL和添加生物炭可将堆肥周期从76天缩短至45天。添加15%的生物炭使速效磷增加到1882 mg kg-1,但使全氮减少到10.5 g kg-1。bsfl -生物炭堆肥中的大肠杆菌水平下降到可接受的限度。结论BSFL和生物炭混合堆肥可缩短堆肥周期,提高堆肥质量。
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引用次数: 4
Hydrolysis of fish waste using fruit wastes of Ananas comosus and Carica papaya for the formulation of liquid fertilizers 用菊苣和番木瓜果渣水解鱼渣制备液体肥料
IF 1.5 Q1 Agricultural and Biological Sciences Pub Date : 2021-02-20 DOI: 10.30486/IJROWA.2021.1891960.1034
R. Ranasinghe, Btsdp Kannagara, R. Ratnayake
PurposeFish waste is a protein-rich source that can be used as a value-added product in the formulation of organic liquid fertilizers. This study carried out to investigate the utilizing potential of fruit wastes of Ananas comosus and Carica papaya that contain bromelain and papain as major proteases in order to hydrolyze fish waste proteins to fulfill plant nitrogen requirements.  MethodProteases were extracted from A. comosus (leaves, crown, unripe fruit pulp, ripe fruit peels and pulp) and C. papaya (leaves, ripe fruit peels and unripe fruit peels). The optimum temperature and pH for the enzymatic activities were determined. Four liquid organic fertilizers were produced with hydrolyzed fish waste that enriched by adding Gliricidia sepium, Chromolaena odorata, Tithonia diversifolia, Mikania scandens and coconut husk-ash. Fertilizers were tested on the growth of Basella alba comparing with a standard fertilizer.  Results Ananas comosus ripe fruit peels and the mixture of (A. comosus + C. papaya) showed the highest enzyme activities (0.33±0.02 and 0.36±0.01 U mL-1 enzyme respectively) at 55 oC and 70 oC. The optimum pH for all the studied extracts was 7.5 at 37 oC. The highest plant fresh and dry weights were recorded in the foliar-applied fertilizer produced by hydrolyzing the fish waste using A. comosus and C. papaya, showing no significant differences to the standard fertilizer.  Conclusion Hydrolysis of fish waste using the fruit wastes ofA. comosus and C. papaya could be effectively used as an organic fertilizer for the growth of B. alba that leads towards sustainable waste management.
目的鱼粪是一种富含蛋白质的来源,可以作为有机液体肥料配方中的增值产品。本研究旨在探讨以菠萝蛋白酶和木瓜蛋白酶为主要蛋白酶的菊苣(Ananas comosus)和番木瓜(Carica papaya)果实废弃物在水解鱼类废弃物蛋白以满足植物氮需求方面的利用潜力。方法分别从山茱萸(叶片、树冠、未成熟果肉、成熟果皮和果肉)和木瓜(叶片、成熟果皮和未成熟果皮)中提取蛋白酶。确定了酶活性的最佳温度和pH。以鱼粪为原料,在鱼粪中添加水杨花、臭臭草、金柳、薇甘菊和椰壳灰,进行了液体有机肥的生产。与标准肥料进行了白僵菌生长试验。结果在55℃和70℃时,菖蒲成熟果皮和菖蒲+番木瓜的混合物酶活性最高,分别为0.33±0.02和0.36±0.01 U mL-1。所有提取物在37℃下的最适pH为7.5。鱼粪水解液和木瓜叶施肥料的植株鲜重和干重最高,与标准肥料差异不显著。结论利用牛蒡果渣水解鱼废。菊苣和番木瓜可以有效地作为白藻生长的有机肥,从而实现废物的可持续管理。
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引用次数: 1
期刊
International Journal Of Recycling of Organic Waste in Agriculture
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