Pub Date : 2018-02-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.67.24
H. Hue, L. T. Nghia, Hoàng Tuyết Minh, L. Anh, L. Trang, T. Khanh
Analysis of genetic diversity of 90 Vietnamese local-colored rice accessions was evaluated by using 40 SSR markers. The numbers of polymorphic alleles ranged from 3 to 12 alleles per locus and average of 7.1 alleles per locus. The similarity coefficients of the rice landraces fluctuated from 0.76 to 0.93; at a genetic correlation level of 0.78. Ninety accessions of rice landraces were divided into five groups based on analysis of genetic relationships. The results have indicated that 11 markers included: M250, RM302, RM10926, RM208, RM227, RM17231, RM23251, RM5647, RM1376, RM339 and RM228 which gave the unique allele. These markers can be used effectively for genetic diversity of colored rice and provided a specific database and useful materials for landraces identification, local germplasm conservation for further colored rice improvement on rice quality via rice breeding programs in Vietnam.
{"title":"Evaluation of Genetic Diversity of Local-Colored Rice Landraces Using SSR Markers","authors":"H. Hue, L. T. Nghia, Hoàng Tuyết Minh, L. Anh, L. Trang, T. Khanh","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.67.24","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.67.24","url":null,"abstract":"Analysis of genetic diversity of 90 Vietnamese local-colored rice accessions was evaluated by using 40 SSR markers. The numbers of polymorphic alleles ranged from 3 to 12 alleles per locus and average of 7.1 alleles per locus. The similarity coefficients of the rice landraces fluctuated from 0.76 to 0.93; at a genetic correlation level of 0.78. Ninety accessions of rice landraces were divided into five groups based on analysis of genetic relationships. The results have indicated that 11 markers included: M250, RM302, RM10926, RM208, RM227, RM17231, RM23251, RM5647, RM1376, RM339 and RM228 which gave the unique allele. These markers can be used effectively for genetic diversity of colored rice and provided a specific database and useful materials for landraces identification, local germplasm conservation for further colored rice improvement on rice quality via rice breeding programs in Vietnam.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48290534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.67.35
R. F. Asafa, W. Akanbi
The experiments were conducted at the Teaching and Research Farm, Ladoke Akintola University of Technology, Ogbomoso, Nigeria in 2010 and 2011 to determine the effect of propagule size and nitrogen levels on the growth and rhizome yield of ginger. The nitrogen source was from Tithonia compost (TC). Three propagule sizes were tested (20, 30 and 40 g) and seven nitrogen levels (0, 40, 60, 80, 100, 120 and 140g kg N/ha). The experimental pots were laid out in a randomized complete block design with three replicates. Data were collected on growth parameters (plant height, number of leaves, leaf length and leaf width); rhizome yield components (number of tillers and rhizomes, and rhizome weight and length); and proximate composition (crude protein (CP), crude fibre (CF), total lipid (TL); Total Ash (TA), Starch and water soluble extract). Data collected were subjected to analysis of variance and significant means were compared using Duncan Multiple Range Test (DMRT) (P ≤ 0.05). Propagule size, N levels and their interactions significantly (P≤ 0.05) influenced most of the parameters assessed. In most cases, the use of 30 and 40 g planting material gave similar results. Plants nourished with 140 kg N/ha significantly and consistently outperformed others fertilized with other nitrogen levels. Crude protein obtained from 40 g propagule size was significantly ( P ≤ 0.05) higher than other tested propagule sizes. The rhizome yields of fertilized plants were significantly higher than the non fertilized plants. Rhizome yields ranged from 1.57 t/ha in none fertilized plants to 3.71 t/ha in 140 kg N/ha. In conclusion, establishment of ginger with 40 g planting materials and its nourishment with 140 kg N/ha enhanced its growth, rhizome yield and proximate compositions of the plant.
实验于2010年和2011年在尼日利亚Ogbomoso Ladoke Akintola理工大学的教学和研究农场进行，以确定繁殖体大小和氮水平对生姜生长和根茎产量的影响。氮的来源为铁香堆肥（TC）。测试了三种繁殖体大小（20、30和40 g）和七种氮水平（0、40、60、80、100、120和140 g kg N/ha）。实验罐采用随机完全块设计，有三个重复。收集有关生长参数（株高、叶数、叶长和叶宽）的数据；根茎产量组成部分（分蘖和根茎的数量，以及根茎的重量和长度）；以及近似组成（粗蛋白（CP）、粗纤维（CF）、总脂质（TL）；总灰分（TA）、淀粉和水溶性提取物）。对收集的数据进行方差分析，并使用Duncan多范围检验（DMRT）比较显著均值（P≤0.05）。繁殖体大小、氮水平及其相互作用显著影响（P≤0.05%）评估的大多数参数。在大多数情况下，使用30克和40克的种植材料会产生类似的结果。用140公斤氮/公顷营养的植物显著且持续地优于用其他氮水平施肥的植物。从40g繁殖体大小获得的粗蛋白显著（P≤0.05）高于其他测试繁殖体大小。受精植株的根茎产量显著高于未受精植株。根状茎产量从未施肥植物的1.57吨/公顷到140公斤N/公顷的3.71吨/公顷不等。综上所述，40g种植材料和140kg N/ha营养液对生姜的生长、根茎产量和主要成分均有促进作用。
{"title":"Growth and Rhizome Yield of Ginger (Zingiber officinale L.) as Influenced by Propagule Size and Nitrogen Levels in Ogbomoso, Southwestern Nigeria","authors":"R. F. Asafa, W. Akanbi","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.67.35","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.67.35","url":null,"abstract":"The experiments were conducted at the Teaching and Research Farm, Ladoke Akintola University of Technology, Ogbomoso, Nigeria in 2010 and 2011 to determine the effect of propagule size and nitrogen levels on the growth and rhizome yield of ginger. The nitrogen source was from Tithonia compost (TC). Three propagule sizes were tested (20, 30 and 40 g) and seven nitrogen levels (0, 40, 60, 80, 100, 120 and 140g kg N/ha). The experimental pots were laid out in a randomized complete block design with three replicates. Data were collected on growth parameters (plant height, number of leaves, leaf length and leaf width); rhizome yield components (number of tillers and rhizomes, and rhizome weight and length); and proximate composition (crude protein (CP), crude fibre (CF), total lipid (TL); Total Ash (TA), Starch and water soluble extract). Data collected were subjected to analysis of variance and significant means were compared using Duncan Multiple Range Test (DMRT) (P ≤ 0.05). Propagule size, N levels and their interactions significantly (P≤ 0.05) influenced most of the parameters assessed. In most cases, the use of 30 and 40 g planting material gave similar results. Plants nourished with 140 kg N/ha significantly and consistently outperformed others fertilized with other nitrogen levels. Crude protein obtained from 40 g propagule size was significantly ( P ≤ 0.05) higher than other tested propagule sizes. The rhizome yields of fertilized plants were significantly higher than the non fertilized plants. Rhizome yields ranged from 1.57 t/ha in none fertilized plants to 3.71 t/ha in 140 kg N/ha. In conclusion, establishment of ginger with 40 g planting materials and its nourishment with 140 kg N/ha enhanced its growth, rhizome yield and proximate compositions of the plant.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42412458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-11-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.66.1
Abdülmelik Aras, Muzaffer Silinsin, Muhammed Nuri Bingol, E. Bursal
The main objective of the current work is to search the antioxidant activities of Origanum acutidens leaves by using DPPH, FRAP and CUPRAC methods severally. Three well known antioxidant compounds (BHA, BHT and ascorbic acid) were used as standards for comparing the extracts. Also, phenolic compounds of Origanum acutidens leaves were identified by UHPLC-ESI-MS/MS. The high concentrations of rosmarinic acid (11158.99 ppb) quinic acid (3200.84 ppb) and naringenin (1238.45 ppb) were detected quantitatively.
{"title":"Identification of Bioactive Polyphenolic Compounds and Assessment of Antioxidant Activity of Origanum acutidens","authors":"Abdülmelik Aras, Muzaffer Silinsin, Muhammed Nuri Bingol, E. Bursal","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.66.1","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.66.1","url":null,"abstract":"The main objective of the current work is to search the antioxidant activities of Origanum acutidens leaves by using DPPH, FRAP and CUPRAC methods severally. Three well known antioxidant compounds (BHA, BHT and ascorbic acid) were used as standards for comparing the extracts. Also, phenolic compounds of Origanum acutidens leaves were identified by UHPLC-ESI-MS/MS. The high concentrations of rosmarinic acid (11158.99 ppb) quinic acid (3200.84 ppb) and naringenin (1238.45 ppb) were detected quantitatively.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45307254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-11-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.66.9
O. Marenkov
Black-striped pipefish Syngnathus abaster Risso, 1827 (Actinopterygii: Syngnathidae) has successfully spread in freshwater ecosystems of Ponto-Caspian basin. Monitoring studies show a steady increase in the number and biomass of pipefish in waters of Dnipro region. It is highly adaptable and breeds in a fresh water. This fact indicates the physiological plasticity of the reproductive system and ability to adapt to new environmental conditions. In the Zaporizke Reservoir pipefish spawns at a temperature of 18.0–22.0°C in the coastal area of the reservoir. Pipefish with a body length of 8.9 to 12.5 cm had gonads with weight of about 0.13 g, and the number of eggs ranged from 26 to 68. The maximum fertility had female 155 mm long and it reached 69 eggs. Size of eggs reached about 1.2±0.05 mm. The histological analysis of the gonads of pipefish and research of histometric measurements of eggs of fish at various stages of development was first conducted. In the period before pipefish spawning, there were eggs in different stages of maturity in the ovaries: oocyte in the phase of primary follicle «С», oocyte in the phase of vacuolation «D3», oocyte in the phase of trophoplasmatic growth «Е», beginning phase of mature follicle «F». Research of pipefish reproduction has important theoretical and practical significance for resolving issues with the spread of this species and its adaptation to new conditions.
{"title":"Aspects of the Reproductive Biology of Black-Striped Pipefish Syngnathus abaster Risso, 1827 (Actinopterygii: Syngnathidae) in the Zaporizke Reservoir, Ukraine","authors":"O. Marenkov","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.66.9","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.66.9","url":null,"abstract":"Black-striped pipefish Syngnathus abaster Risso, 1827 (Actinopterygii: Syngnathidae) has successfully spread in freshwater ecosystems of Ponto-Caspian basin. Monitoring studies show a steady increase in the number and biomass of pipefish in waters of Dnipro region. It is highly adaptable and breeds in a fresh water. This fact indicates the physiological plasticity of the reproductive system and ability to adapt to new environmental conditions. In the Zaporizke Reservoir pipefish spawns at a temperature of 18.0–22.0°C in the coastal area of the reservoir. Pipefish with a body length of 8.9 to 12.5 cm had gonads with weight of about 0.13 g, and the number of eggs ranged from 26 to 68. The maximum fertility had female 155 mm long and it reached 69 eggs. Size of eggs reached about 1.2±0.05 mm. The histological analysis of the gonads of pipefish and research of histometric measurements of eggs of fish at various stages of development was first conducted. In the period before pipefish spawning, there were eggs in different stages of maturity in the ovaries: oocyte in the phase of primary follicle «С», oocyte in the phase of vacuolation «D3», oocyte in the phase of trophoplasmatic growth «Е», beginning phase of mature follicle «F». Research of pipefish reproduction has important theoretical and practical significance for resolving issues with the spread of this species and its adaptation to new conditions.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42125734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-11-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.66.17
Obianuju Orimma Kalu, P. Ojimelukwe, A. Ukom
We evaluated the carotenoid profile and concentration (by HPLC) and the phytochemical content of two OFSP varieties (Umuspo 3 and Ex-Igbariam) planted at three distances (20 cm, 30 cm and 40 cm) and harvested in two different periods (12th and 16th weeks after planting) respectively. Carotene contents of the outer peel and inner flesh of the sweet potato varieties were also determined. The results showed wide variation in the carotenoid and phytochemical content among the varieties at different planting spaces and harvest periods. Umuspo 3 planted at 20 cm, 30 cm and 40 cm had significantly greater carotenoid concentration than Ex-Igbariam variety. The predominant carotenoid was β-carotene with highest concentration obtained from 40 cm planting distance (92.82µg/g) and 30 cm (80.97µg/g) for Umuspo 3. Ex-Igbariam at 30 cm planting distance contained 2.51µg/g β-carotene when harvested after 16th weeks. Also the highest β-carotene concentration was from Umuspo 3 flesh sample planted 30 and 40 cm (409.45 and 441.15 mg/100g) and the peel for samples planted 30 and 40 cm (490.47 and 640.69 mg/100g, respectively) at the 12th week of harvest. Flavonoids were present in significant amounts (310.62mg/100g) in Umuspo 3 planted at 30 cm and harvested after 12th week while in total polyphenol, significant quantities of ≈42.12mg/100g was present in Ex-Igbariam spaced at 30 cm and 40 cm and harvested after 16th week. Provitamin A carotenoid was calculated and Umuspo 3 pro-vitamin A carotenoid was significantly higher (p< 0.05) with highest concentration (742.26 RE/100g) present in samples from 40 cm planting distance. The results showed that planting space and harvesting period had significant impact on the carotenoid and phytochemical concentrations of OFSP varieties. Planting distances of 30 and 40 cm are recommended for high carotenoid content in the two sweet potato varieties.
{"title":"Evaluation of the Effect of Planting Distance and Harvesting Time on the Carotenoids and Phytochemicals of Selected Orange-Fleshed Sweet Potato Varieties","authors":"Obianuju Orimma Kalu, P. Ojimelukwe, A. Ukom","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.66.17","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.66.17","url":null,"abstract":"We evaluated the carotenoid profile and concentration (by HPLC) and the phytochemical content of two OFSP varieties (Umuspo 3 and Ex-Igbariam) planted at three distances (20 cm, 30 cm and 40 cm) and harvested in two different periods (12th and 16th weeks after planting) respectively. Carotene contents of the outer peel and inner flesh of the sweet potato varieties were also determined. The results showed wide variation in the carotenoid and phytochemical content among the varieties at different planting spaces and harvest periods. Umuspo 3 planted at 20 cm, 30 cm and 40 cm had significantly greater carotenoid concentration than Ex-Igbariam variety. The predominant carotenoid was β-carotene with highest concentration obtained from 40 cm planting distance (92.82µg/g) and 30 cm (80.97µg/g) for Umuspo 3. Ex-Igbariam at 30 cm planting distance contained 2.51µg/g β-carotene when harvested after 16th weeks. Also the highest β-carotene concentration was from Umuspo 3 flesh sample planted 30 and 40 cm (409.45 and 441.15 mg/100g) and the peel for samples planted 30 and 40 cm (490.47 and 640.69 mg/100g, respectively) at the 12th week of harvest. Flavonoids were present in significant amounts (310.62mg/100g) in Umuspo 3 planted at 30 cm and harvested after 12th week while in total polyphenol, significant quantities of ≈42.12mg/100g was present in Ex-Igbariam spaced at 30 cm and 40 cm and harvested after 16th week. Provitamin A carotenoid was calculated and Umuspo 3 pro-vitamin A carotenoid was significantly higher (p< 0.05) with highest concentration (742.26 RE/100g) present in samples from 40 cm planting distance. The results showed that planting space and harvesting period had significant impact on the carotenoid and phytochemical concentrations of OFSP varieties. Planting distances of 30 and 40 cm are recommended for high carotenoid content in the two sweet potato varieties.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42658245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.65.16
M. Awasthi, Pragya Gupta, F. Bano, M. Serajuddin
Juvenile of Trichogaster lalius was reared under three photoperiod conditions (0L:24D, 12L:12D, 16L:08D) for 60 days to examine the growth performance under laboratory conditions. The maximum growth was observed in the juvenile of T. lalius exposed to 16:00 hrs. light. The mean body weight of different groups of juvenile fish exposed to different light conditions were significantly different (p<0.05) from each other which was observed from 20thday of the experiment. The absolute, specific, and relative growth rates were found to be maximum in the group exposed to 16:00 hrs. light duration. The present study indicated that 16:00 hrs. light duration was considered to be better for the growth of juvenile of T. lalius under controlled condition.
{"title":"Effects of Photoperiods on the Growth Performance of Juvenile Trichogaster lalius (Hamilton, 1822)","authors":"M. Awasthi, Pragya Gupta, F. Bano, M. Serajuddin","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.65.16","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.65.16","url":null,"abstract":"Juvenile of Trichogaster lalius was reared under three photoperiod conditions (0L:24D, 12L:12D, 16L:08D) for 60 days to examine the growth performance under laboratory conditions. The maximum growth was observed in the juvenile of T. lalius exposed to 16:00 hrs. light. The mean body weight of different groups of juvenile fish exposed to different light conditions were significantly different (p<0.05) from each other which was observed from 20thday of the experiment. The absolute, specific, and relative growth rates were found to be maximum in the group exposed to 16:00 hrs. light duration. The present study indicated that 16:00 hrs. light duration was considered to be better for the growth of juvenile of T. lalius under controlled condition.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45080859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.65.1
T. Iusypiva, G. Miasoid
The paper examines the influence of industrial emissions of sulphur (IV) and nitrogen (IV) oxides on the percentage of stem anatomical characteristics of the autochthonous woody plant species undergrowth of Acer platanoides L. and Fraxinus excelsior L. in the southern industrial zone of the city of Dnipro (Ukraine). It is ascertained, that the ratio of the primary cortex share to the central cylinder share does not change in the stems of annual shoots of the both studied woody plant species when they are exposed to the influence of the toxic gases. However, there are significant changes in the ratio of shares of histological elements in the undergrowth stems of F. excelsior. The use of both absolute and relevant values of anatomic parameters of the plant vegetative organs is needed to analyse the woody plants resistance to technogenic pollution of the environment.
{"title":"The Impact of Industrial Pollution on the Stem Anatomical Characteristics of Woody Plant Undergrowth in the City of Dnipro, Ukraine","authors":"T. Iusypiva, G. Miasoid","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.65.1","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.65.1","url":null,"abstract":"The paper examines the influence of industrial emissions of sulphur (IV) and nitrogen (IV) oxides on the percentage of stem anatomical characteristics of the autochthonous woody plant species undergrowth of Acer platanoides L. and Fraxinus excelsior L. in the southern industrial zone of the city of Dnipro (Ukraine). It is ascertained, that the ratio of the primary cortex share to the central cylinder share does not change in the stems of annual shoots of the both studied woody plant species when they are exposed to the influence of the toxic gases. However, there are significant changes in the ratio of shares of histological elements in the undergrowth stems of F. excelsior. The use of both absolute and relevant values of anatomic parameters of the plant vegetative organs is needed to analyse the woody plants resistance to technogenic pollution of the environment.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46675633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.65.10
H. Tebyanian, A. Bakhtiari, A. Karami, A. Kariminik
Probiotics have antibacterial effects against pathogenic bacteria in the gut while maintaining the balance of intestinal flora such as Lactobacillus. This study aimed to evaluate the antimicrobial activity of four Lactobacillus species against intestinal pathogenic. Four different species of Lactobacillus (Lactobacillus bulgaricus (PTCC 1332), Lactobacillus casei (PTCC 1608), Lactobacillus plantarum (PTCC 1058) and Lactobacillus Fermentum (PTCC 1638)) were experimented to investigate the inhibitory activity against 4 bacterial enteric pathogens (Escherichia coli, Staphylococcus aureus, Shigella dysenteriae and Salmonella paratyphi A) which were separately inoculated in MRS medium (de Man, Rogosa and Sharpe medium) for 48 hours at 37 °C and pH 7. Our results showed that enteropathogens growth was stopped in the presence of all Lactobacillus and inhibition zone was between 12 and 32 millimeter. It can be concluded that these four Lactobacillus strains had potential antimicrobial compounds against human enteric pathogens and should be further studied for their human health benefits.
{"title":"Antimicrobial Activity of some Lactobacillus Species against Intestinal Pathogenic Bacteria","authors":"H. Tebyanian, A. Bakhtiari, A. Karami, A. Kariminik","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.65.10","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.65.10","url":null,"abstract":"Probiotics have antibacterial effects against pathogenic bacteria in the gut while maintaining the balance of intestinal flora such as Lactobacillus. This study aimed to evaluate the antimicrobial activity of four Lactobacillus species against intestinal pathogenic. Four different species of Lactobacillus (Lactobacillus bulgaricus (PTCC 1332), Lactobacillus casei (PTCC 1608), Lactobacillus plantarum (PTCC 1058) and Lactobacillus Fermentum (PTCC 1638)) were experimented to investigate the inhibitory activity against 4 bacterial enteric pathogens (Escherichia coli, Staphylococcus aureus, Shigella dysenteriae and Salmonella paratyphi A) which were separately inoculated in MRS medium (de Man, Rogosa and Sharpe medium) for 48 hours at 37 °C and pH 7. Our results showed that enteropathogens growth was stopped in the presence of all Lactobacillus and inhibition zone was between 12 and 32 millimeter. It can be concluded that these four Lactobacillus strains had potential antimicrobial compounds against human enteric pathogens and should be further studied for their human health benefits.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41454230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.64.1
A. Elzaawely, H. Maswada, M. El-sayed, M. Ahmed
Agricultural wastes cause a serious environmental problem in Egypt. Utilization of these wastes by an environmentally friendly way is a very important issue. The objective of this study was to utilize rice straw into high-value products. Antioxidant activity, total soluble phenols, and flavonoids were evaluated in ethyl acetate extract prepared from rice straw after alkaline hydrolysis. Total phenols were found to be 221.6 mg gallic acid equivalents (GAE), while total flavonoids were 4.9 mg rutin equivalents (RE). The extract exhibited strong antioxidant activity measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and its IC50 value was 0.4 mg/ml. Furthermore, the ethyl acetate extract possessed high antioxidant activity assayed by β-carotene bleaching method and its value of lipid peroxidation inhibition (LPI) was 75.4%. It also exhibited high reducing power and its IC50 value was equal to 0.06 mg/ml. HPLC analysis indicated that this extract contained seven phenolic acids including; protocatechuic, caffeic, syringic, p-coumaric, ferulic, rosmarinic and cinnamic, in addition to two flavonoids; qurecetin and kaempferol. Results indicated that ferulic and p-coumaric acids were the major soluble phenolic acids in rice straw, and their concentrations were 3.9 and 2.9 mg/g DW. It can be summarized that the strong antioxidant activity of ethyl acetate extract, prepared from rice straw, was highly correlated with its high level of phenolic compounds.
{"title":"Phenolic Compounds and Antioxidant Activity of Rice Straw Extract","authors":"A. Elzaawely, H. Maswada, M. El-sayed, M. Ahmed","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.64.1","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.64.1","url":null,"abstract":"Agricultural wastes cause a serious environmental problem in Egypt. Utilization of these wastes by an environmentally friendly way is a very important issue. The objective of this study was to utilize rice straw into high-value products. Antioxidant activity, total soluble phenols, and flavonoids were evaluated in ethyl acetate extract prepared from rice straw after alkaline hydrolysis. Total phenols were found to be 221.6 mg gallic acid equivalents (GAE), while total flavonoids were 4.9 mg rutin equivalents (RE). The extract exhibited strong antioxidant activity measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and its IC50 value was 0.4 mg/ml. Furthermore, the ethyl acetate extract possessed high antioxidant activity assayed by β-carotene bleaching method and its value of lipid peroxidation inhibition (LPI) was 75.4%. It also exhibited high reducing power and its IC50 value was equal to 0.06 mg/ml. HPLC analysis indicated that this extract contained seven phenolic acids including; protocatechuic, caffeic, syringic, p-coumaric, ferulic, rosmarinic and cinnamic, in addition to two flavonoids; qurecetin and kaempferol. Results indicated that ferulic and p-coumaric acids were the major soluble phenolic acids in rice straw, and their concentrations were 3.9 and 2.9 mg/g DW. It can be summarized that the strong antioxidant activity of ethyl acetate extract, prepared from rice straw, was highly correlated with its high level of phenolic compounds.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41637568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.64.32
C. Ishiwu, I. Onoh, Peace Ogechi Nwanya, A. Agulanna
Starch was extracted (isolated) from cocoyam with the aid of water solution of oxalic acid and ammonium oxalate in 8 samples of ratios, 1:3, 1:1, 3:1, 2:3, 2:1, 1:2 and 0:0 respectively. The physiochemical properties were investigated in order to unveil its characteristics and unravel the potentials for industrial applications of the cocoyam starch. The physiochemical properties investigated includes; Amylose and Amylopectin contents, water binding capacity, particle size distribution, swelling power and solubility. The results obtained showed that swelling power and solubility of the starch were temperature dependent. The solubility was found to increase with temperature increase as the cocoyam starch showed highest solubility within the 70-90°C temperature range. The swelling power was found fluctuating between the temperatures of 25-90°C. The swelling power starch sample isolated with blending ratios of 2:1 and 1:2 were temperature dependent. The Amylose content ranges from 3.06 to 31.21%.
{"title":"Physiochemical Properties of Cocoyam Starch Extracted in Two Media","authors":"C. Ishiwu, I. Onoh, Peace Ogechi Nwanya, A. Agulanna","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.64.32","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.64.32","url":null,"abstract":"Starch was extracted (isolated) from cocoyam with the aid of water solution of oxalic acid and ammonium oxalate in 8 samples of ratios, 1:3, 1:1, 3:1, 2:3, 2:1, 1:2 and 0:0 respectively. The physiochemical properties were investigated in order to unveil its characteristics and unravel the potentials for industrial applications of the cocoyam starch. The physiochemical properties investigated includes; Amylose and Amylopectin contents, water binding capacity, particle size distribution, swelling power and solubility. The results obtained showed that swelling power and solubility of the starch were temperature dependent. The solubility was found to increase with temperature increase as the cocoyam starch showed highest solubility within the 70-90°C temperature range. The swelling power was found fluctuating between the temperatures of 25-90°C. The swelling power starch sample isolated with blending ratios of 2:1 and 1:2 were temperature dependent. The Amylose content ranges from 3.06 to 31.21%.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45568076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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