One of the most popular and useable of Aromatic plants are kinds of Basilica (Ocimum sp., Lamiaceae). This genius has different characteristics in behavior germination. Biodynamic agriculture is a new science in the research and especially, the position of stars and planet relative to each other, position of moon around the earth and seasonal change and Solar and lunar eclipses. We must to demonstrative of this theory. In this experiment, we want to record the effect of based on two calendars (biodynamic and Astronomic) on 3 ecotype of Basilica seed germination. The based on this experiment randomized block design with 3 replications. We use 50 seeds in Falcon tube (value=15ml) and record the weight and value of seed in tube. Then added 5ml of water in tube and shacked after moisturized and control and record of Mucilage value and another behavior of seed germination in lab condition. Results showed that different position of some planets such as moon and Mars and Jupiter had the highest effect, positive and significant effect on mucilage percent (p>95%), germination speed and length of root. Therefore, we could be express the lunar position had the highest effect on root length of local type and the lowest effect on purple basil. Mucilage percentage of seed coat in green basil had the highest content relative to another ecotype and this content was significant (p>95%). Then, the best suggest for produce of mucilage from the basil seeds, the best time is the first quadrature of moon.
{"title":"Investigation of the Biodynamic Commands Use Effect on Mucilage Content and Germination Behavior in Three Ecotypes of Basil (Ocimum Sp.)","authors":"M. Sabet Teimouri","doi":"10.56431/p-9g6w2z","DOIUrl":"https://doi.org/10.56431/p-9g6w2z","url":null,"abstract":"One of the most popular and useable of Aromatic plants are kinds of Basilica (Ocimum sp., Lamiaceae). This genius has different characteristics in behavior germination. Biodynamic agriculture is a new science in the research and especially, the position of stars and planet relative to each other, position of moon around the earth and seasonal change and Solar and lunar eclipses. We must to demonstrative of this theory. In this experiment, we want to record the effect of based on two calendars (biodynamic and Astronomic) on 3 ecotype of Basilica seed germination. The based on this experiment randomized block design with 3 replications. We use 50 seeds in Falcon tube (value=15ml) and record the weight and value of seed in tube. Then added 5ml of water in tube and shacked after moisturized and control and record of Mucilage value and another behavior of seed germination in lab condition. Results showed that different position of some planets such as moon and Mars and Jupiter had the highest effect, positive and significant effect on mucilage percent (p>95%), germination speed and length of root. Therefore, we could be express the lunar position had the highest effect on root length of local type and the lowest effect on purple basil. Mucilage percentage of seed coat in green basil had the highest content relative to another ecotype and this content was significant (p>95%). Then, the best suggest for produce of mucilage from the basil seeds, the best time is the first quadrature of moon.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2019-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47017381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.73.17
N. Ryzhenko
In this paper, the influence of trace metals(Cd, Pb, Cu, Co, Ni, Zn) on plants of spring barley (Hordeum vulgare L.) was investigated in polluted sod podzolic sandy loam on layered glacial sands and calcareous deep chernozem on loamy loess soils. We propose to estimate the phytotoxicity with help of phytotoxicological classification. The phytotoxicological classification of trace metals gives the possibility to assess their hazard for plants. On the base of indicators such as: plant up-taking index (UI), phytoletal dose (PhLD50), Dipole moment (µ), Phyto Maximum Allowable Concentration (PMAC) a phytotoxicological classification of hazardous trace metals was suggested. The four classes of danger in phytotoxicological classification of hazardous trace metals were offered. According to phytotoxicological classification, Cd, Co, Ni belong to the first class of hazard, Cu – to second class of hazard, Zn – to third class of hazard, Pb – to fourth class of hazard. Phytotoxicological classification of hazardous trace metals gives the possibility to comprehensively estimate the danger of trace metals for plants as a biological object that plays a very important role in the life of ecosystem. This approach may be applied for another trace metals risk assessment for other plants.
{"title":"Metals Phytotoxicity Assessment and Classification","authors":"N. Ryzhenko","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.73.17","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.73.17","url":null,"abstract":"In this paper, the influence of trace metals(Cd, Pb, Cu, Co, Ni, Zn) on plants of spring barley (Hordeum vulgare L.) was investigated in polluted sod podzolic sandy loam on layered glacial sands and calcareous deep chernozem on loamy loess soils. We propose to estimate the phytotoxicity with help of phytotoxicological classification. The phytotoxicological classification of trace metals gives the possibility to assess their hazard for plants. On the base of indicators such as: plant up-taking index (UI), phytoletal dose (PhLD50), Dipole moment (µ), Phyto Maximum Allowable Concentration (PMAC) a phytotoxicological classification of hazardous trace metals was suggested. The four classes of danger in phytotoxicological classification of hazardous trace metals were offered. According to phytotoxicological classification, Cd, Co, Ni belong to the first class of hazard, Cu – to second class of hazard, Zn – to third class of hazard, Pb – to fourth class of hazard. Phytotoxicological classification of hazardous trace metals gives the possibility to comprehensively estimate the danger of trace metals for plants as a biological object that plays a very important role in the life of ecosystem. This approach may be applied for another trace metals risk assessment for other plants.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48745011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.73.26
Ramin Rayee, T. Xuan, Kifayatullah Kakar, M. I. Haqani
The present study was conducted to evaluate the antioxidant activity, grain traits and quality contents in Afghanistan’s selected rice varieties. The antioxidant capacity was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl) and Reducing Power methods. Grain length, grain width and grain thickness were in the range of 5.4-7.6, 1.7-3.2, and 1.5-2.2 mm respectively. Selah Zoodras, Taram Irani and Attai-1 had the longest grain length whereas Luke was associated with high width and thickness among the varieties. Protein, amylose and lipid content varied from 6.5-9.9, 20.3-23.5, and 10-18% respectively. Barah Kunduz showed the highest protein and lipid content while Selah zoodras was associated highest amylose content. The selected rice varieties possessed moderate antioxidant activity and their physiochemical properties were not correlated with antioxidant capacity. Results of this study provided useful information on the selection and production of rice varieties with high nutritional and pharmaceutical values in Afghanistan.
{"title":"Antioxidant Activity, Quality Parameters and Grain Characteristics of Rice Varieties of Afghanistan","authors":"Ramin Rayee, T. Xuan, Kifayatullah Kakar, M. I. Haqani","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.73.26","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.73.26","url":null,"abstract":"The present study was conducted to evaluate the antioxidant activity, grain traits and quality contents in Afghanistan’s selected rice varieties. The antioxidant capacity was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl) and Reducing Power methods. Grain length, grain width and grain thickness were in the range of 5.4-7.6, 1.7-3.2, and 1.5-2.2 mm respectively. Selah Zoodras, Taram Irani and Attai-1 had the longest grain length whereas Luke was associated with high width and thickness among the varieties. Protein, amylose and lipid content varied from 6.5-9.9, 20.3-23.5, and 10-18% respectively. Barah Kunduz showed the highest protein and lipid content while Selah zoodras was associated highest amylose content. The selected rice varieties possessed moderate antioxidant activity and their physiochemical properties were not correlated with antioxidant capacity. Results of this study provided useful information on the selection and production of rice varieties with high nutritional and pharmaceutical values in Afghanistan.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44595599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.73.36
M. Teimouri
One of the most popular and useable of Aromatic plants are kinds of Basilica (Ocimumsp., Lamiaceae). This genius has different characteristics in behavior germination. Biodynamic agriculture is a new science in the research and especially, the position of stars and planet relative to each other, position of moon around the earth and seasonal change and Solar and lunar eclipses. We must to demonstrative of this theory. In this experiment, we want to record the effect of based on two calendars (biodynamic and Astronomic) on 3 ecotype of Basilica seed germination. The based on this experiment randomized block design with 3 replications. We use 50 seeds in Falcon tube (value=15ml) and record the weight and value of seed in tube. Then added 5ml of water in tube and shacked after moisturized and control and record of Mucilage value and another behavior of seed germination in lab condition. Results showed that different position of some planets such as moon and Mars and Jupiter had the highest effect, positive and significant effect on mucilage percent (p>95%), germination speed and length of root. Therefore, we could be express the lunar position had the highest effect on root length of local type and the lowest effect on purple basil. Mucilage percentage of seed coat in green basil had the highest content relative to another ecotype and this content was significant (p>95%). Then, the best suggest for produce of mucilage from the basil seeds, the best time is the first quadrature of moon.
{"title":"Investigation of the Biodynamic Commands Use Effect on Mucilage Content and Germination Behavior in Three Ecotypes of Basil (Ocimum Sp.)","authors":"M. Teimouri","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.73.36","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.73.36","url":null,"abstract":"One of the most popular and useable of Aromatic plants are kinds of Basilica (Ocimumsp., Lamiaceae). This genius has different characteristics in behavior germination. Biodynamic agriculture is a new science in the research and especially, the position of stars and planet relative to each other, position of moon around the earth and seasonal change and Solar and lunar eclipses. We must to demonstrative of this theory. In this experiment, we want to record the effect of based on two calendars (biodynamic and Astronomic) on 3 ecotype of Basilica seed germination. The based on this experiment randomized block design with 3 replications. We use 50 seeds in Falcon tube (value=15ml) and record the weight and value of seed in tube. Then added 5ml of water in tube and shacked after moisturized and control and record of Mucilage value and another behavior of seed germination in lab condition. Results showed that different position of some planets such as moon and Mars and Jupiter had the highest effect, positive and significant effect on mucilage percent (p>95%), germination speed and length of root. Therefore, we could be express the lunar position had the highest effect on root length of local type and the lowest effect on purple basil. Mucilage percentage of seed coat in green basil had the highest content relative to another ecotype and this content was significant (p>95%). Then, the best suggest for produce of mucilage from the basil seeds, the best time is the first quadrature of moon.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47187384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.73.1
E. Desoky, A. Elrys, M. Rady
Natural supplementations are used in agriculture nowadays not only for improving plant performance but also for reducing the contamination of plant edible parts. Two field trials were conducted to study the potential effects of licorice root extract (LRE; 0.5%) on performance, physio-biochemical components, antioxidant defense system, and contaminants concentrations ofCapsicum annuumL. plants grown on a saline soil contaminated with heavy metals. LRE was applied in single (i.e., as rhizosphere application with drip irrigation water; -RA or as foliar spray; -FA) or in integration (i.e., LRE-RA + LRE-FA) treatment. The results showed that both single or integrative treatments significantly increased plant growth and yield, leaf concentrations of photosynthetic pigments, free proline, total soluble sugars, N, P, and K+, ratio of K+/Na+, and activities of CAT, POX, APX, SOD and GR, while significantly reduced contaminants; Na+, Cd, Cu, Pb and Ni concentrations in plant leaves and fruits on heavy metals-contaminated saline soil compared to the control (without LRE). Additionally, the integrative LRE-RA + LRE-FA treatment significantly exceeded both single treatments in this concern, which had been recommended for maximizing pepper plant performances with minimizing heavy metals in fruits on contaminated saline soils.
{"title":"Licorice Root Extract Boosts Capsicum annuum L. Production and Reduces Fruit Contamination on a Heavy Metals-Contaminated Saline Soil","authors":"E. Desoky, A. Elrys, M. Rady","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.73.1","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.73.1","url":null,"abstract":"Natural supplementations are used in agriculture nowadays not only for improving plant performance but also for reducing the contamination of plant edible parts. Two field trials were conducted to study the potential effects of licorice root extract (LRE; 0.5%) on performance, physio-biochemical components, antioxidant defense system, and contaminants concentrations ofCapsicum annuumL. plants grown on a saline soil contaminated with heavy metals. LRE was applied in single (i.e., as rhizosphere application with drip irrigation water; -RA or as foliar spray; -FA) or in integration (i.e., LRE-RA + LRE-FA) treatment. The results showed that both single or integrative treatments significantly increased plant growth and yield, leaf concentrations of photosynthetic pigments, free proline, total soluble sugars, N, P, and K+, ratio of K+/Na+, and activities of CAT, POX, APX, SOD and GR, while significantly reduced contaminants; Na+, Cd, Cu, Pb and Ni concentrations in plant leaves and fruits on heavy metals-contaminated saline soil compared to the control (without LRE). Additionally, the integrative LRE-RA + LRE-FA treatment significantly exceeded both single treatments in this concern, which had been recommended for maximizing pepper plant performances with minimizing heavy metals in fruits on contaminated saline soils.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43702637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.72.7
M. Rady, A. A. El-Shewy, M. El-Yazal, Kareman E.S. Abdelaal
The study objective is to evaluate the effect of mono-ammonuim phosphate (MAP; 0, 10, and 20 mM) applied as foliar application on the growth traits, green and dry yields characteristics, leaf photosynthetic pigments, chlorophyll fluorescence, and leaf contents of nutrients of common bean (Phaseolus vulgaris L., cv. “Bronco”) plants grown under saline soil conditions. To perform this objective, two field trials were conducted at the Experimental Farm of Faculty of Agriculture, Fayoum University during the 2016 and 2017 summer seasons. The obtained results showed that, Na+ content was significantly declined, while the all other tested parameters such as growth characteristics (i.e., shoot length, number of leaves per plant, area of leaves per plant, and shoot fresh and dry weights), yield characteristics of green pods and dry seeds (i.e., average pod weight, number of pods per plant, pods weight per plant, dry seed weight per plant and 100-seed weight), leaf photosynthetic pigments (i.e., total chlorophylls, total carotenoids) contents and leaf chlorophyll fluorescence (i.e., Fv/Fm and PI), leaf contents of N, P, K+, and Ca2+, and the ratios of K+/Na+, Ca2+/Na+ and K++Ca2+/Na+ were significantly increased by the two levels (i.e., 10 and 20 mM) of MAP compared to the controls (without MAP). The two MAP levels conferred the same results for most of the all tested parameters; particularly growth and yields characteristics, with some exceptions. Therefore, results of this study recommend using 10 mM MAP as foliar application to optimize the common bean performances in saline soils. Keywords: Common beans, Salinity, Phosphorus, Plant performance, Antioxidant defense systems, Photosynthesis, Water relations.
{"title":"Response of Salt-Stressed Common Bean Plant Performances to Foliar Application of Phosphorus (MAP)","authors":"M. Rady, A. A. El-Shewy, M. El-Yazal, Kareman E.S. Abdelaal","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.72.7","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.72.7","url":null,"abstract":"The study objective is to evaluate the effect of mono-ammonuim phosphate (MAP; 0, 10, and 20 mM) applied as foliar application on the growth traits, green and dry yields characteristics, leaf photosynthetic pigments, chlorophyll fluorescence, and leaf contents of nutrients of common bean (Phaseolus vulgaris L., cv. “Bronco”) plants grown under saline soil conditions. To perform this objective, two field trials were conducted at the Experimental Farm of Faculty of Agriculture, Fayoum University during the 2016 and 2017 summer seasons. The obtained results showed that, Na+ content was significantly declined, while the all other tested parameters such as growth characteristics (i.e., shoot length, number of leaves per plant, area of leaves per plant, and shoot fresh and dry weights), yield characteristics of green pods and dry seeds (i.e., average pod weight, number of pods per plant, pods weight per plant, dry seed weight per plant and 100-seed weight), leaf photosynthetic pigments (i.e., total chlorophylls, total carotenoids) contents and leaf chlorophyll fluorescence (i.e., Fv/Fm and PI), leaf contents of N, P, K+, and Ca2+, and the ratios of K+/Na+, Ca2+/Na+ and K++Ca2+/Na+ were significantly increased by the two levels (i.e., 10 and 20 mM) of MAP compared to the controls (without MAP). The two MAP levels conferred the same results for most of the all tested parameters; particularly growth and yields characteristics, with some exceptions. Therefore, results of this study recommend using 10 mM MAP as foliar application to optimize the common bean performances in saline soils. Keywords: Common beans, Salinity, Phosphorus, Plant performance, Antioxidant defense systems, Photosynthesis, Water relations.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49412110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.72.37
Ja'afar Umar, A. A. Aliero, K. Shehu, L. Abubakar
Abstract Salinity inhibition of plant growth is the result of osmotic and ionic effect and different plant species have developed different mechanisms to cope with those effects. With the discovery of molecular markers and marker assisted selection technology, it is possible to develop markers that identify salt tolerance. The genetic diversity of tomato genotypes were analyzed using SSRs polymorphic markers and Unweighted Pair Group Method with Arithmetic Mean. Leaves of the twenty tomato genotypes (landraces/accessions in Nigeria) were used to isolate their DNA using Bioland Plant Genomic DNA protocols. Primers were designed from 15 different salt responsive candidate genes, using Vector NTI and the sequence genes were obtained from ncbi genomice web site. All 15 primers sets generated shows clear distinct polymorphic profiles as evident from the 6% agarose gel profile. Dendrogram generated shows three groups, none of the panel intermixed in a subgroup. The genetic distance information reported in this study might be used by breeders when planning future crosses among tomato genotypes. From the result obtained UC82B recorded the highest vegetative and yield parameters, therefore, adoption of this genotype could be help increasing the tomato production in Sokoto agro-climatic area. Abstract Salinity inhibition of plant growth is the result of osmotic and ionic effect and different plant species have developed different mechanisms to cope with those effects. With the discovery of molecular markers and marker assisted selection technology, it is possible to develop markers that identify salt tolerance. The genetic diversity of tomato genotypes were analyzed using SSRs polymorphic markers and Unweighted Pair Group Method with Arithmetic Mean. Leaves of the twenty tomato genotypes (landraces/accessions in Nigeria) were used to isolate their DNA using Bioland Plant Genomic DNA protocols. Primers were designed from 15 different salt responsive candidate genes, using Vector NTI and the sequence genes were obtained from ncbi genomice web site. All 15 primers sets generated shows clear distinct polymorphic profiles as evident from the 6% agarose gel profile. Dendrogram generated shows three groups, none of the panel intermixed in a subgroup. The genetic distance information reported in this study might be used by breeders when planning future crosses among tomato genotypes. From the result obtained UC82B recorded the highest vegetative and yield parameters, therefore, adoption of this genotype could be help increasing the tomato production in Sokoto agro-climatic area.
{"title":"Genetic Diversity in Tomato Genotypes (Solanum lycopersicum) Based on Salinity Responsive Candidate Gene Using Simple Sequence Repeats","authors":"Ja'afar Umar, A. A. Aliero, K. Shehu, L. Abubakar","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.72.37","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.72.37","url":null,"abstract":"Abstract Salinity inhibition of plant growth is the result of osmotic and ionic effect and different plant species have developed different mechanisms to cope with those effects. With the discovery of molecular markers and marker assisted selection technology, it is possible to develop markers that identify salt tolerance. The genetic diversity of tomato genotypes were analyzed using SSRs polymorphic markers and Unweighted Pair Group Method with Arithmetic Mean. Leaves of the twenty tomato genotypes (landraces/accessions in Nigeria) were used to isolate their DNA using Bioland Plant Genomic DNA protocols. Primers were designed from 15 different salt responsive candidate genes, using Vector NTI and the sequence genes were obtained from ncbi genomice web site. All 15 primers sets generated shows clear distinct polymorphic profiles as evident from the 6% agarose gel profile. Dendrogram generated shows three groups, none of the panel intermixed in a subgroup. The genetic distance information reported in this study might be used by breeders when planning future crosses among tomato genotypes. From the result obtained UC82B recorded the highest vegetative and yield parameters, therefore, adoption of this genotype could be help increasing the tomato production in Sokoto agro-climatic area. Abstract Salinity inhibition of plant growth is the result of osmotic and ionic effect and different plant species have developed different mechanisms to cope with those effects. With the discovery of molecular markers and marker assisted selection technology, it is possible to develop markers that identify salt tolerance. The genetic diversity of tomato genotypes were analyzed using SSRs polymorphic markers and Unweighted Pair Group Method with Arithmetic Mean. Leaves of the twenty tomato genotypes (landraces/accessions in Nigeria) were used to isolate their DNA using Bioland Plant Genomic DNA protocols. Primers were designed from 15 different salt responsive candidate genes, using Vector NTI and the sequence genes were obtained from ncbi genomice web site. All 15 primers sets generated shows clear distinct polymorphic profiles as evident from the 6% agarose gel profile. Dendrogram generated shows three groups, none of the panel intermixed in a subgroup. The genetic distance information reported in this study might be used by breeders when planning future crosses among tomato genotypes. From the result obtained UC82B recorded the highest vegetative and yield parameters, therefore, adoption of this genotype could be help increasing the tomato production in Sokoto agro-climatic area.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48634390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.72.21
L. Cheban, Iryna Malishchuk, M. Marchenko
Various studies of the components of the antioxidant protection system of microalgae D. armatus under the influence of osmotic stress and active forms of oxygen will allow to develop methods for controlling carotenogenesis in a given culture and to obtain carotenoid enriched feed for zooplankton. These studies made it possible to evaluate the activity of catalase, peroxidase enzymes in cells that are cultured under the induction of carotenogenesis by free radical oxidation promoters and osmotic stress on the background of physiological changes. It is established that under these conditions, there is an increase in volumes and aggregation of vegetative cells. At the same time, the amount of biomass remains at the level of the first day of inductors application. Against the background of a decrease in growth activity, a decrease in the number of metabolically active cells in cytochrome oxidase was observed. It is also shown that, when iron sulfate is introduced with hydrogen peroxide and sodium chloride against the background of enhanced carotenogenesis, antioxidant systems are activated by increasing the activity of catalase and peroxidase. Under such conditions, it is possible to achieve increased production of carotenoids in Desmodesmus armatus culture.
{"title":"Reaction of Cells Desmodesmus armatus (Chod.) Hegew. on the Induction of Carotynogenesis","authors":"L. Cheban, Iryna Malishchuk, M. Marchenko","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.72.21","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.72.21","url":null,"abstract":"Various studies of the components of the antioxidant protection system of microalgae D. armatus under the influence of osmotic stress and active forms of oxygen will allow to develop methods for controlling carotenogenesis in a given culture and to obtain carotenoid enriched feed for zooplankton. These studies made it possible to evaluate the activity of catalase, peroxidase enzymes in cells that are cultured under the induction of carotenogenesis by free radical oxidation promoters and osmotic stress on the background of physiological changes. It is established that under these conditions, there is an increase in volumes and aggregation of vegetative cells. At the same time, the amount of biomass remains at the level of the first day of inductors application. Against the background of a decrease in growth activity, a decrease in the number of metabolically active cells in cytochrome oxidase was observed. It is also shown that, when iron sulfate is introduced with hydrogen peroxide and sodium chloride against the background of enhanced carotenogenesis, antioxidant systems are activated by increasing the activity of catalase and peroxidase. Under such conditions, it is possible to achieve increased production of carotenoids in Desmodesmus armatus culture.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43867311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.72.28
Muhammed Nuri Bingol, E. Bursal
The identification and quantification of phenolic compounds of Stachys lavandulifolia Vahl. var. brachydon Boiss. by LC-MS/MS (Liquid Chromatography- tandem Mass Spectrometry) technique is the main purpose of the current study. The high concentrations of quinic acid (2534±12 ppb) and chlorogenic acid (1882±92 ppb) were detected by LC-MS/MS.. Another goal of the study is to evaluate the antioxidant activities of both ethanol and aqueous extracts of the plant material. The antioxidant potentials of extracts were determined by using five different in vitro methods including; ABTS (2,2′-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), DPPH(1,1-diphenyl-2-picryl-hydrazyl), FRAP (Ferric ions Reducing Antioxidant Power), CUPRAC (Cupric ions Reducing Antioxidant Power), and ferric thiocyanate methods. The results revealed that the aqueous and ethanol extracts of S. lavandulifolia leaves have good antioxidant potential with high phenolic content.
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Pub Date : 2018-11-01DOI: 10.18052/WWW.SCIPRESS.COM/ILNS.72.1
A. Dayan, N. Sarı, F. Özoğul
In this study, Nusem and Beppo snack seed pumpkin cultivars were used to determine the effects of different plant activators on seed protein, lipid and fatty acids contents. In the context of study, plant activators consist of Crop-set (CR), EM1, ERS, Vitormone-Plus Drip (VIT), Bacillus subtilis (OSU 142), Bacillus megatorium (M3), Azospirillum sp. (SP 245), Spirulina platensis (SIP), Ecocompost (EKO), Camli Botanica liquid organic fertilizer (BOT) and Zincon (ZIN) were used as organic fertilizer. In the experiment, the plant activators were applied to the plants alone or in combination with each other and organic fertilizer. Two separate control groups which were organic and conventional (CONV.) fertilizer have been identified. As a result of the use of different plant activators, the highest protein content was obtained from CONV. application (35.50%), M3+SP 245 (33.09%) and M3 (33.04%); the highest lipid content was observed from SP 245+OG (45.90%), CR (44.48%) and SIP+OG (44.26%) applications. The use of different plant activators effected the fatty acid contents of seeds. Total 11 fatty acids were identified. Among the fatty acids, C16:0 (Palmitic acid), C18:0 (Stearic acid), C18:1 (Oleic acid) and C18:2 (Linoleic acid) were found dominant.
本研究以Nusem和Beppo休闲南瓜种子品种为材料，测定了不同植物激活剂对种子蛋白质、脂质和脂肪酸含量的影响。在研究背景下，使用由Crop set（CR）、EM1、ERS、Vitormone Plus Drip（VIT）、枯草芽孢杆菌（OSU 142）、巨型芽孢杆菌（M3）、氮螺菌属（sp 245）、螺旋藻（SIP）、生态堆肥（EKO）、Camli Botanica液体有机肥（BOT）和Zincon（ZIN）组成的植物活化剂作为有机肥料。在实验中，将植物活化剂单独或与有机肥料联合施用于植物。已经确定了两个单独的对照组，即有机肥料和常规肥料。由于使用了不同的植物激活剂，从CONV.施用中获得了最高的蛋白质含量（35.50%）、M3+SP245（33.09%）和M3（33.04%）；从SP 245+OG（45.90%）、CR（44.48%）和SIP+OG（44.26%）应用中观察到最高的脂质含量。不同植物激活剂的使用对种子的脂肪酸含量有影响。共鉴定出11种脂肪酸。脂肪酸中，棕榈酸C16:0、硬脂酸C18:0、油酸C18:1和亚油酸C18:2占优势。
{"title":"The Effects of Different Plant Activators on Protein, Lipid and Fatty Acids in Snack-Seed Pumpkin","authors":"A. Dayan, N. Sarı, F. Özoğul","doi":"10.18052/WWW.SCIPRESS.COM/ILNS.72.1","DOIUrl":"https://doi.org/10.18052/WWW.SCIPRESS.COM/ILNS.72.1","url":null,"abstract":"In this study, Nusem and Beppo snack seed pumpkin cultivars were used to determine the effects of different plant activators on seed protein, lipid and fatty acids contents. In the context of study, plant activators consist of Crop-set (CR), EM1, ERS, Vitormone-Plus Drip (VIT), Bacillus subtilis (OSU 142), Bacillus megatorium (M3), Azospirillum sp. (SP 245), Spirulina platensis (SIP), Ecocompost (EKO), Camli Botanica liquid organic fertilizer (BOT) and Zincon (ZIN) were used as organic fertilizer. In the experiment, the plant activators were applied to the plants alone or in combination with each other and organic fertilizer. Two separate control groups which were organic and conventional (CONV.) fertilizer have been identified. As a result of the use of different plant activators, the highest protein content was obtained from CONV. application (35.50%), M3+SP 245 (33.09%) and M3 (33.04%); the highest lipid content was observed from SP 245+OG (45.90%), CR (44.48%) and SIP+OG (44.26%) applications. The use of different plant activators effected the fatty acid contents of seeds. Total 11 fatty acids were identified. Among the fatty acids, C16:0 (Palmitic acid), C18:0 (Stearic acid), C18:1 (Oleic acid) and C18:2 (Linoleic acid) were found dominant.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48540196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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