Maize crop is the third most important cereal crop, mostly grown for food, feed and fodder purpose. In spite of the fact the crop is susceptible to salt stress but exhibits a considerable genotypic variability for salt tolerance. The present study was carried out to determine to which extent the maize genotypes with contrasting morphological traits respond to salt stress. Seven days old seedlings of thirty maize genotypes were exposed to NaCl stress of EC less than 4 (control), equal to 8 and 12 dS m-1 for further 28 days in a temperature controlled greenhouse. The salt stress imposition was completed in two increments every other day from the date of planting. At the age of 35 days, the seedlings were evaluated for contrasting morphological traits. The genotypes exhibited considerable variations for each of the 10 measured morphological and ionic traits. PCA was employed to identify the most suitable morphological trait to be used as selection criterion for salt tolerance. Based on the PCA results, dry shoot weight (DSW) was used to classify thirty maize genotypes into salt-tolerant-T, moderately tolerant-MT and salt sensitive-S groups. Two (2) out of thirty genotypes i.e. SB-9617 and FH-949 had the highest average of percent dry shoot weight (PDSW) values > 70% were classified as salt tolerant (T). The fifteen (15) genotypes showed average of PDSW values in the range of 55-69.9 % were classified as moderately tolerant (MT) by indicating the sequence order of salt tolerance as YH-1898 > MMRI-yellow > S-2002 > FH-988 > FH-1292 > HC-12 > MS-2018 > NCEV-1270-7 > Pahari > Pearl White > FH-1114 > NCEV-1270-3 > Iqbal > NCEV-1297 > DK-6724. However, thirteen (13) out of total thirty (30) genotypes attained the average PDSW values <55% were classified as salt sensitive (S) following the sequence order as NCEV-1530-9 > Composite > FRI-22 > Azam > MS-2015 > P-1543 > Neelum > Afgoi > Malka-2016 > MS-1501 > HNG > NCEV-1270-5 > NCEV-7004. It was also noticed that the declared salt tolerance was positively correlated with dry shoot weight (DSW), shoot length (SL), stem diameter (SD), fresh shoot weight (FSW), fresh root weight (FRW), dry roots weight (DRW), root length (RL), leaf area per plant (LA), number of leaves per plant (NL) and K+/Na+ ratio. The highest positive Pearson correlation coefficients were determined in LA vs SD (r=0.900), DSW vs SD (r=0.899), SL vs DSW (r=0.891), SL vs FSW (r=0.890) and DSW vs FSW (r=0.887). Additionally, the salt tolerant (T) group of genotypes maintained higher K+/Na+ ratios compared to moderately tolerant (MT) and salt sensitive (S) groups. The results clearly showed that dry shoot weight (DSW) could be viable option to classify maize genotypes into different salt tolerance groups and to identify the most suited and best adapted salt tolerant genotype for cultivation in saline soils. Furthermore, this scientific information could help the plant scientists to improve and develop the new salt tolerant cultivar.
玉米是第三大谷物作物，主要用于食品、饲料和饲料。尽管作物易受盐胁迫，但耐盐性表现出相当大的基因型变异。本研究旨在确定具有相反形态特征的玉米基因型对盐胁迫的反应程度。30个玉米基因型的7天龄幼苗在温控温室中暴露于EC小于4（对照）、等于8和12dSm-1的NaCl胁迫28天。从种植之日起，每隔一天以两个增量完成盐胁迫施加。在35天龄时，对幼苗进行对比形态特征评估。基因型在10个测量的形态和离子性状中的每一个都表现出相当大的变异。采用主成分分析法确定了最适合作为耐盐性选择标准的形态性状。基于主成分分析结果，利用干茎重（DSW）将30个玉米基因型分为耐盐性T组、耐中度MT组和耐盐性S组。30个基因型中，SB-9617和FH-949中有两（2）个具有最高平均干枝重百分比（PDSW）值>70%的基因型被归类为耐盐（T）。十五（15）个基因型的PDSW值平均在55-69.9%之间，通过表明耐盐性的顺序为YH-1898>MMRI黄>S-2002>FH-988>FH-1292>HC-12>MS-2018>NCEV-1270-7>Pahari>珍珠白>FH-1114>NCEV-1270-3>Iqbal>NCEV-1297>DK-6724，将其归类为中度耐受（MT）。然而，在总共三十（30）个基因型中，有十三（13）个获得了平均PDSW值Composite>FRI-22>Azam>MS-2015>P-1543>Neelum>Afgoi>Malka-2016>MS-1501>HNG>NCEV-1270-5>NCEV-7004。还注意到，所宣布的耐盐性与干茎重（DSW）、茎长（SL）、茎径（SD）、鲜茎重（FSW）、鲜根重（FRW）、干根重（DRW）、根长（RL）、单株叶面积（LA）、单株叶片数（NL）和K+/Na+比率呈正相关。LA与SD（r=0.900）、DSW与SD（r=0.899）、SL与DSW（r=0.891）、SL vs FSW（r=0.8 90）和DSW与FSW（r=0.887）的Pearson正相关系数最高。此外，与中度耐受（MT）和盐敏感（S）组相比，耐盐（T）基因型组保持较高的K+/Na+比率。结果清楚地表明，干茎重（DSW）可以将玉米基因型分为不同的耐盐组，并确定最适合和最适合在盐碱地种植的耐盐基因型。此外，这些科学信息可以帮助植物科学家改进和开发耐盐新品种。
{"title":"Characterizing Maize Genotypes for Salt Tolerance Using Morphological and Ionic Traits at Seedling Stage","authors":"S. S. Hussain, M. Rasheed, Z. I. Ahmed, G. Jilani","doi":"10.56431/p-03pzu8","DOIUrl":"https://doi.org/10.56431/p-03pzu8","url":null,"abstract":"Maize crop is the third most important cereal crop, mostly grown for food, feed and fodder purpose. In spite of the fact the crop is susceptible to salt stress but exhibits a considerable genotypic variability for salt tolerance. The present study was carried out to determine to which extent the maize genotypes with contrasting morphological traits respond to salt stress. Seven days old seedlings of thirty maize genotypes were exposed to NaCl stress of EC less than 4 (control), equal to 8 and 12 dS m-1 for further 28 days in a temperature controlled greenhouse. The salt stress imposition was completed in two increments every other day from the date of planting. At the age of 35 days, the seedlings were evaluated for contrasting morphological traits. The genotypes exhibited considerable variations for each of the 10 measured morphological and ionic traits. PCA was employed to identify the most suitable morphological trait to be used as selection criterion for salt tolerance. Based on the PCA results, dry shoot weight (DSW) was used to classify thirty maize genotypes into salt-tolerant-T, moderately tolerant-MT and salt sensitive-S groups. Two (2) out of thirty genotypes i.e. SB-9617 and FH-949 had the highest average of percent dry shoot weight (PDSW) values > 70% were classified as salt tolerant (T). The fifteen (15) genotypes showed average of PDSW values in the range of 55-69.9 % were classified as moderately tolerant (MT) by indicating the sequence order of salt tolerance as YH-1898 > MMRI-yellow > S-2002 > FH-988 > FH-1292 > HC-12 > MS-2018 > NCEV-1270-7 > Pahari > Pearl White > FH-1114 > NCEV-1270-3 > Iqbal > NCEV-1297 > DK-6724. However, thirteen (13) out of total thirty (30) genotypes attained the average PDSW values <55% were classified as salt sensitive (S) following the sequence order as NCEV-1530-9 > Composite > FRI-22 > Azam > MS-2015 > P-1543 > Neelum > Afgoi > Malka-2016 > MS-1501 > HNG > NCEV-1270-5 > NCEV-7004. It was also noticed that the declared salt tolerance was positively correlated with dry shoot weight (DSW), shoot length (SL), stem diameter (SD), fresh shoot weight (FSW), fresh root weight (FRW), dry roots weight (DRW), root length (RL), leaf area per plant (LA), number of leaves per plant (NL) and K+/Na+ ratio. The highest positive Pearson correlation coefficients were determined in LA vs SD (r=0.900), DSW vs SD (r=0.899), SL vs DSW (r=0.891), SL vs FSW (r=0.890) and DSW vs FSW (r=0.887). Additionally, the salt tolerant (T) group of genotypes maintained higher K+/Na+ ratios compared to moderately tolerant (MT) and salt sensitive (S) groups. The results clearly showed that dry shoot weight (DSW) could be viable option to classify maize genotypes into different salt tolerance groups and to identify the most suited and best adapted salt tolerant genotype for cultivation in saline soils. Furthermore, this scientific information could help the plant scientists to improve and develop the new salt tolerant cultivar.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43893368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shahbaz Khan, M. Khan, D. Ibrar, S. Irshad, Nabila Rashid, Madad Ali
This research was carried out at Khyber Pakhtunkhwa Agricultural University Peshawar to evaluate the performance of maize inbred lines derived from maize variety Sarhad White regarding grain yield and morphological traits and identifying superior inbred lines based on test cross performance. Test crosses were evaluated in replicated trial along with two tester, one check and 16 parental inbred lines for comparison in a 7×7 partially balanced lattice square design with two replications. Results revealed highly significant differences among the genotypes for most of the traits. Jalal TC-230-a and TC-63-1 took the maximum days to tasseling and Jalal TC-101-2 showed maximum days to anthesis. Maximum day to silking and anthesis silking interval were recorded in Jalal TC-94. Maximum plant height was observed in Jalal TC-33 (164.5 cm) and Kiramat TC-9-6-1 (141.50 cm). Maximum 100 kernal weight was recorded in Jalal TC-101-2 (40.55 g). Kiramat TC-89-b produced the highest grain yield (6808 kg ha-1). From these results, it can be concluded that Kiramat TC-89-b, having highest yield, could be promoted for further selfing. Jalal TC-9-6, TC-9-6-1, TC-12-1, TC-63-1, TC-76-2b, TC-89b, TC-163, TC-205, TC-230a, TC-211 and Kiramat TC-9-6, TC-9-6-1, TC-12-1, TC-33, TC-76-2b, TC-89b, TC-94, TC-101-2, 150-c, TC-163, TC-230-a, 205, 211, 226-2 in addition to having satisfactory grain yield could be used for selfing. Among parental lines (selfed lines) 9-6-1, 9-6, 10-2, 226-2, 33, 63-1, 76-2, 163, 89-b, 205 and 211 were promising for grain yield and morphological traits.
{"title":"Performance of Test Crosses of Maize Variety Sarhad White with Jalal and Kiramat for Morphological Traits","authors":"Shahbaz Khan, M. Khan, D. Ibrar, S. Irshad, Nabila Rashid, Madad Ali","doi":"10.56431/p-d2a433","DOIUrl":"https://doi.org/10.56431/p-d2a433","url":null,"abstract":"This research was carried out at Khyber Pakhtunkhwa Agricultural University Peshawar to evaluate the performance of maize inbred lines derived from maize variety Sarhad White regarding grain yield and morphological traits and identifying superior inbred lines based on test cross performance. Test crosses were evaluated in replicated trial along with two tester, one check and 16 parental inbred lines for comparison in a 7×7 partially balanced lattice square design with two replications. Results revealed highly significant differences among the genotypes for most of the traits. Jalal TC-230-a and TC-63-1 took the maximum days to tasseling and Jalal TC-101-2 showed maximum days to anthesis. Maximum day to silking and anthesis silking interval were recorded in Jalal TC-94. Maximum plant height was observed in Jalal TC-33 (164.5 cm) and Kiramat TC-9-6-1 (141.50 cm). Maximum 100 kernal weight was recorded in Jalal TC-101-2 (40.55 g). Kiramat TC-89-b produced the highest grain yield (6808 kg ha-1). From these results, it can be concluded that Kiramat TC-89-b, having highest yield, could be promoted for further selfing. Jalal TC-9-6, TC-9-6-1, TC-12-1, TC-63-1, TC-76-2b, TC-89b, TC-163, TC-205, TC-230a, TC-211 and Kiramat TC-9-6, TC-9-6-1, TC-12-1, TC-33, TC-76-2b, TC-89b, TC-94, TC-101-2, 150-c, TC-163, TC-230-a, 205, 211, 226-2 in addition to having satisfactory grain yield could be used for selfing. Among parental lines (selfed lines) 9-6-1, 9-6, 10-2, 226-2, 33, 63-1, 76-2, 163, 89-b, 205 and 211 were promising for grain yield and morphological traits.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48304159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yield reduction in okra production is mostly associated with cultivation of marginal lands or continuous land cultivation. Hence, the need for sustainable approach to resuscitating soil fertility through Tithonia diversifolia manure application may alter microbial population. Two soil depths [Topsoil (0-15 cm) and Subsoil (15-30 cm)], three levels of Tithonia manure applications (control, half recommended and recommended at 120 kg N/ha) and three okras varieties (Clemson spineless, LD88 and NHAe 47-4) were evaluated in completely randomized design replicated thrice. Soil bacterial and fungal colonies were higher in the topsoil compared to the subsoil and increased with increase in level of tithonia manure application under topsoil, while the inverse was observed under subsoil condition for bacteria colony-forming units and mycorrhizal spore count. Under topsoil, LD88 variety treated with recommended rate of tithonia manure had the highest biomass dry matter (79.4 g). Significantly higher leaf area (395.92 mm2) and biomass dry matter (66.89 g) were produced by NHAe 47-4 and Clemson spineless treated with recommended rate of tithonia manure compared to their respective controls (181.37 mm2 and 29.63 g) under subsoil. Varieties LD88 and Clemson spineless combined with recommended rates of tithonia manure were suggested for topsoil and subsoil conditions, respectively.
{"title":"Responses of Okra and Soil Microbial Population Changes to the Application of Tithonia Manure\u0000 ","authors":"Tajudeen Akinrinola, I. Nwagboso, O. Fagbola","doi":"10.56431/p-8l2von","DOIUrl":"https://doi.org/10.56431/p-8l2von","url":null,"abstract":"Yield reduction in okra production is mostly associated with cultivation of marginal lands or continuous land cultivation. Hence, the need for sustainable approach to resuscitating soil fertility through Tithonia diversifolia manure application may alter microbial population. Two soil depths [Topsoil (0-15 cm) and Subsoil (15-30 cm)], three levels of Tithonia manure applications (control, half recommended and recommended at 120 kg N/ha) and three okras varieties (Clemson spineless, LD88 and NHAe 47-4) were evaluated in completely randomized design replicated thrice. Soil bacterial and fungal colonies were higher in the topsoil compared to the subsoil and increased with increase in level of tithonia manure application under topsoil, while the inverse was observed under subsoil condition for bacteria colony-forming units and mycorrhizal spore count. Under topsoil, LD88 variety treated with recommended rate of tithonia manure had the highest biomass dry matter (79.4 g). Significantly higher leaf area (395.92 mm2) and biomass dry matter (66.89 g) were produced by NHAe 47-4 and Clemson spineless treated with recommended rate of tithonia manure compared to their respective controls (181.37 mm2 and 29.63 g) under subsoil. Varieties LD88 and Clemson spineless combined with recommended rates of tithonia manure were suggested for topsoil and subsoil conditions, respectively.\u0000 ","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2022-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47298493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Huslystyi, V. Nedzvetsky, S. Yermolenko, V. Gasso, V. Petrushevskyi, E. Sukharenko
Imidacloprid is a widely used pesticide that belongs to the class of neonicotinoids. There is a piece of rising evidence that neonicotinoids exert cytotoxic effects in non-target organisms including vertebrate species such as mammals. Nevertheless, dose-limiting toxicity and molecular mechanisms of neonicotinoids' deleterious effects are still poorly understood. In accord to imidacloprid fate in the environment, the most of used pesticide is absorbed in the soil. Therefore, earthworms, which are prevailing soil organisms, could be considered as a target of neonicotinoids toxicity. The earthworm’s simple nervous system is a prospective model for neurotoxicological studies. We exposed earthworms to imidacloprid in a paper contact test with a doses range of 0.1‑0.4 µg/cm2 for 14 days. In the present work, we studied the imidacloprid effect on oxidative stress generation and neuronal marker neuron-specific enolase (NSE) expression. The exposure to imidacloprid induced a dose-dependent decrease in NSE. Both reactive oxygen species production and lipid peroxidation level were upregulated as well. Observed NSE decline suggests imidacloprid-caused disturbance in earthworm neuron cells. Obtained data have shown that relatively low doses of imidacloprid are potent to induce cytotoxicity in neurons. Furthermore, neurotoxicity could be recognized as one of an individual scenario of the general imidacloprid toxicity. Thus, presented results suggest the cytotoxicity of imidacloprid low doses in non-target organisms and hypothesize that NSE downregulation could be estimated as a biomarker of neonicotinoid cytotoxicity in a nervous system of non-insect species.
{"title":"Low Doses of Imidacloprid Induce Oxidative Stress and Neural Cell Disruption in Earthworm Eisenia fetida","authors":"A. Huslystyi, V. Nedzvetsky, S. Yermolenko, V. Gasso, V. Petrushevskyi, E. Sukharenko","doi":"10.56431/p-af973e","DOIUrl":"https://doi.org/10.56431/p-af973e","url":null,"abstract":"Imidacloprid is a widely used pesticide that belongs to the class of neonicotinoids. There is a piece of rising evidence that neonicotinoids exert cytotoxic effects in non-target organisms including vertebrate species such as mammals. Nevertheless, dose-limiting toxicity and molecular mechanisms of neonicotinoids' deleterious effects are still poorly understood. In accord to imidacloprid fate in the environment, the most of used pesticide is absorbed in the soil. Therefore, earthworms, which are prevailing soil organisms, could be considered as a target of neonicotinoids toxicity. The earthworm’s simple nervous system is a prospective model for neurotoxicological studies. We exposed earthworms to imidacloprid in a paper contact test with a doses range of 0.1‑0.4 µg/cm2 for 14 days. In the present work, we studied the imidacloprid effect on oxidative stress generation and neuronal marker neuron-specific enolase (NSE) expression. The exposure to imidacloprid induced a dose-dependent decrease in NSE. Both reactive oxygen species production and lipid peroxidation level were upregulated as well. Observed NSE decline suggests imidacloprid-caused disturbance in earthworm neuron cells. Obtained data have shown that relatively low doses of imidacloprid are potent to induce cytotoxicity in neurons. Furthermore, neurotoxicity could be recognized as one of an individual scenario of the general imidacloprid toxicity. Thus, presented results suggest the cytotoxicity of imidacloprid low doses in non-target organisms and hypothesize that NSE downregulation could be estimated as a biomarker of neonicotinoid cytotoxicity in a nervous system of non-insect species.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2021-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46760009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Field experiment was conducted in Giza, Egypt, during two growing seasons of 2017-2018 and 2018-2019 on garlic crop, with the objective of investigating the effect of different irrigation levels (60, 80 and 100 % of water requirements and their combination with the foliar spraying applications of agrispon (with 0.5 and 1.0 ml/ liter) on growth and yield. The results indicated that increased irrigation level up to 100% led to increased vegetative characters of garlic and that the lowest growth and productivity was obtained by 60% irrigation level. When considering spray application of agrispon; with 1.0 ml/L increased growth and productivity followed by 0.5 ml/L; while control treatment gave the lowest productivity during the both seasons. Interaction effect between irrigation level and agrispon treatments indicated that 100% irrigation level combined with 1.0 ml/L spray application of agrispon gave the highest garlic productivity followed by 100% irrigation level combined with 0.5 ml/L spray application. The chemical analysis showed that the highest NPK was obtained by 100% irrigation level combined with 1.0 ml/L agrispon application during the both seasons. Regarding water footprint, the highest irrigation water footprint was obtained by 80% irrigation level followed by 60% irrigation level, while the lowest footprint was obtained by 100% irrigation level due to high garlic productivity under 100% irrigation level. The estimate water footprint for garlic was 525 m3/ton. The blue water footprint for garlic was 422 m3/ton about 80% form total water footprint, while gray water percentage about 20% with value of 103 m3/ton.
{"title":"Water Footprint for Garlic under Irrigation Levels and Agrispon Application","authors":"A. Farag, M. Abdrabbo, Z. Maharik, R. El-Morshedy","doi":"10.56431/p-i9ucn3","DOIUrl":"https://doi.org/10.56431/p-i9ucn3","url":null,"abstract":"Field experiment was conducted in Giza, Egypt, during two growing seasons of 2017-2018 and 2018-2019 on garlic crop, with the objective of investigating the effect of different irrigation levels (60, 80 and 100 % of water requirements and their combination with the foliar spraying applications of agrispon (with 0.5 and 1.0 ml/ liter) on growth and yield. The results indicated that increased irrigation level up to 100% led to increased vegetative characters of garlic and that the lowest growth and productivity was obtained by 60% irrigation level. When considering spray application of agrispon; with 1.0 ml/L increased growth and productivity followed by 0.5 ml/L; while control treatment gave the lowest productivity during the both seasons. Interaction effect between irrigation level and agrispon treatments indicated that 100% irrigation level combined with 1.0 ml/L spray application of agrispon gave the highest garlic productivity followed by 100% irrigation level combined with 0.5 ml/L spray application. The chemical analysis showed that the highest NPK was obtained by 100% irrigation level combined with 1.0 ml/L agrispon application during the both seasons. Regarding water footprint, the highest irrigation water footprint was obtained by 80% irrigation level followed by 60% irrigation level, while the lowest footprint was obtained by 100% irrigation level due to high garlic productivity under 100% irrigation level. The estimate water footprint for garlic was 525 m3/ton. The blue water footprint for garlic was 422 m3/ton about 80% form total water footprint, while gray water percentage about 20% with value of 103 m3/ton.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2021-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45537521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-27DOI: 10.18052/www.scipress.com/ilns.83.1
S. Ghabour, S. Mohamed, S. A. S. El-Yazal, H. M. Moawad
The aim of this study was to clarify the phytochemical property evaluation of Roselle plants grown under bio Azotobacterine (Azotobacterchroococcum) and phosphorein (Bacillus polymyxa) and mineral (N, P and K fertilizers at the rates of 25, 50 and 100% from the doses recommended by Ministry of Agriculture) fertilizers applied in different types of soil. Samples of Roselle plants were obtained from different soils (clay soil at Dar El-Ramad farm, sandy loam and saline loamy sand soil at Demo farm, Faculty of Agriculture) at El-Fayoum governorate conditions. Moreover, some of the phytochemical properties (N, P and K percentage in roselle herb and its uptake, photosynthetic pigments (chlorophyll A, chlorophyll B and carotenoids), anthocyanin pigment and pH value) of roselle plants (Hibiscus sabdariffa, L.) under different soils were determined. The data obtained showed that, bio and mineral (NPK) fertilizers increased the above compositions of roselle plants under different soils of experiment. The maximum increase of these compositions was obtained by the treatment clay soil × 100% NPK + bio fertilizers, followed by clay soil × 50% NPK + bio fertilizers as compared to saline loamy sand soil × non fertilizer treatment, although, the differences between these treatments and mineral fertilizer at the rate of 100% NPK alone were insignificantly. Therefore, it is economically and environmentally recommended to inoculate roselle seeds with mixture of Azotobacter + Bacillus and fertilize these inoculated plants with 50% NPK for improve chemical compositions (N, P and K percentage in roselle herb and its uptake, photosynthetic pigments (chlorophyll A, chlorophyll B and carotenoids), anthocyanin pigment and pH value) of roselle plants under clay soil. Key words: Roselle, Hibiscus sabdariffa L., nitrogen, phosphorus, potassium, biofertilization, soil type, salinity, chemical composition.
本研究的目的是阐明在不同类型土壤中施用生物固氮菌（Azotobacterchroococcum）、磷蛋白（Bacillus polymyxa）和矿物肥料（农业部推荐剂量的25%、50%和100%的N、P和K肥料）的玫瑰茄植物的植物化学性质评估。玫瑰茄植物的样品是从El Fayoum省条件下的不同土壤（Dar El Ramad农场的粘土、农业学院Demo农场的沙壤土和含盐壤土）中获得的。此外，还测定了不同土壤条件下玫瑰茄（Hibiscus sabdariffa，L.）的一些植物化学特性（玫瑰茄中N、P、K的百分比及其吸收、光合色素（叶绿素A、叶绿素B和类胡萝卜素）、花青素色素和pH值）。结果表明，在不同的试验土壤条件下，生物和矿物肥料均能提高玫瑰茄植株的上述组成。粘土×100%NPK+生物肥料处理的这些成分增加幅度最大，其次是粘土×50%NPK+生态肥料处理，与含盐壤土×无肥料处理相比，尽管这些处理与单独使用100%NPK的矿物肥料之间的差异不显著。因此，在经济和环境上建议用固氮菌+芽孢杆菌的混合物接种玫瑰茄种子，并用50%的NPK对这些接种的植物进行施肥，以改善玫瑰茄草本植物的化学成分（N、P和K百分比及其吸收、光合色素（叶绿素A、叶绿素B和类胡萝卜素），花青素色素和pH值）。关键词：玫瑰茄，芙蓉，氮，磷，钾，生物肥料，土壤类型，盐度，化学成分。
{"title":"Phytochemical Properties of Roselle (Hibiscus sabdariffa, L.) Plants Grown under Bio and Mineral Fertilizers in Different Types of Soil","authors":"S. Ghabour, S. Mohamed, S. A. S. El-Yazal, H. M. Moawad","doi":"10.18052/www.scipress.com/ilns.83.1","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.83.1","url":null,"abstract":"The aim of this study was to clarify the phytochemical property evaluation of Roselle plants grown under bio Azotobacterine (Azotobacterchroococcum) and phosphorein (Bacillus polymyxa) and mineral (N, P and K fertilizers at the rates of 25, 50 and 100% from the doses recommended by Ministry of Agriculture) fertilizers applied in different types of soil. Samples of Roselle plants were obtained from different soils (clay soil at Dar El-Ramad farm, sandy loam and saline loamy sand soil at Demo farm, Faculty of Agriculture) at El-Fayoum governorate conditions. Moreover, some of the phytochemical properties (N, P and K percentage in roselle herb and its uptake, photosynthetic pigments (chlorophyll A, chlorophyll B and carotenoids), anthocyanin pigment and pH value) of roselle plants (Hibiscus sabdariffa, L.) under different soils were determined. The data obtained showed that, bio and mineral (NPK) fertilizers increased the above compositions of roselle plants under different soils of experiment. The maximum increase of these compositions was obtained by the treatment clay soil × 100% NPK + bio fertilizers, followed by clay soil × 50% NPK + bio fertilizers as compared to saline loamy sand soil × non fertilizer treatment, although, the differences between these treatments and mineral fertilizer at the rate of 100% NPK alone were insignificantly. Therefore, it is economically and environmentally recommended to inoculate roselle seeds with mixture of Azotobacter + Bacillus and fertilize these inoculated plants with 50% NPK for improve chemical compositions (N, P and K percentage in roselle herb and its uptake, photosynthetic pigments (chlorophyll A, chlorophyll B and carotenoids), anthocyanin pigment and pH value) of roselle plants under clay soil. Key words: Roselle, Hibiscus sabdariffa L., nitrogen, phosphorus, potassium, biofertilization, soil type, salinity, chemical composition.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49414197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-27DOI: 10.18052/www.scipress.com/ilns.83.55
A. Makarenko, P. Shevchenko, I. S. Kononenko, Vadym M. Kondratyk, D. Khrystenko, V. Grubinko
The growth of man-caused load on natural objects, including surface waters, gave impetus to the studying for issue of water pollution in Ukraine by various pollutants. Especially relevant for us are the studies of fishery reservoirs as a direct environment for growing quality aquaculture products. Among the toxic compounds contained in reservoirs, one of the first places is occupied by heavy metals. On the one hand, heavy metals, as pollutants in natural waters, pose a great danger because even in relatively small concentrations they can adversely affect aquatic organisms. On the other hand, in microquantities most heavy metals (except mercury, cadmium and lead) are a natural and even essential component of living cells of aquatic organisms, including fish [17,19].Zn, Mn, Fe, Cu, Pb, Co, Ni, Cd in the organs and tissues of the bighead carphybrid form, which was cultivated in ponds from highly urbanized territory, are distributed heterogeneously and their level of content depends not only on the physical and chemical characteristics of the aquatic environment or this heavy metals biochemical activities force in fish organisms, but also on functional features of concrete organs and tissues of the latter. Results of our investigations showed that above mentioned heavy metals were characterised by high levels of content in the organs and tissues of hybrid silver x bigheadcarp. Highest content in organism of hybrid are lead, cobalt, and cadmium. Highest concentration of heavy metals in our investigations was recorded in gills, because it is the first defence line of fish organism from environmental pollutants.
{"title":"Heavy Metals in Organs and Tissues of Silver X Bigheads Carp Hybrid as Indices of Anthropogenic Pressure in Areas with a High Level of Urbanization","authors":"A. Makarenko, P. Shevchenko, I. S. Kononenko, Vadym M. Kondratyk, D. Khrystenko, V. Grubinko","doi":"10.18052/www.scipress.com/ilns.83.55","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.83.55","url":null,"abstract":"The growth of man-caused load on natural objects, including surface waters, gave impetus to the studying for issue of water pollution in Ukraine by various pollutants. Especially relevant for us are the studies of fishery reservoirs as a direct environment for growing quality aquaculture products. Among the toxic compounds contained in reservoirs, one of the first places is occupied by heavy metals. On the one hand, heavy metals, as pollutants in natural waters, pose a great danger because even in relatively small concentrations they can adversely affect aquatic organisms. On the other hand, in microquantities most heavy metals (except mercury, cadmium and lead) are a natural and even essential component of living cells of aquatic organisms, including fish [17,19].Zn, Mn, Fe, Cu, Pb, Co, Ni, Cd in the organs and tissues of the bighead carphybrid form, which was cultivated in ponds from highly urbanized territory, are distributed heterogeneously and their level of content depends not only on the physical and chemical characteristics of the aquatic environment or this heavy metals biochemical activities force in fish organisms, but also on functional features of concrete organs and tissues of the latter. Results of our investigations showed that above mentioned heavy metals were characterised by high levels of content in the organs and tissues of hybrid silver x bigheadcarp. Highest content in organism of hybrid are lead, cobalt, and cadmium. Highest concentration of heavy metals in our investigations was recorded in gills, because it is the first defence line of fish organism from environmental pollutants.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47504108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-27DOI: 10.18052/www.scipress.com/ilns.83.22
J. Vergara, M. Demetillo, L. Ombat, Jashin Rosal
Plants are reservoirs of bioactive compounds with the potential for pharmaceutical use. In this study, the secondary metabolites of Neonauclea formicaria leaf crude ethanolic extract were determined using phytochemical screening. The plant's leaf extract was then used to test its angiogenesis activity using the chorioallantoic membrane (CAM) assay. Four concentrations of the extract were prepared—0.1 mg/L, 1.0 mg/L, 10.0 mg/L, and 100.0 mg/L and were topically applied on the CAM. Phytochemical screening revealed that N. formicaria leaves contain heavy amounts of flavonoids and tannins, while alkaloids, saponins, and steroids were present in trace amounts. The crude ethanolic extract was anti-angiogenic, as indicated by the significant decrease of vascular density at higher concentrations (P<0.05). The 100 mg/L extract concentration showed the highest vascular inhibition (50.93%) among the other concentrations, suggesting its angiopreventive potential (P<0.05). Further investigation on the embryo's gross morphometry revealed no significant effects in the weight, crown-rump length, head-beak length, forelimb length, and hind limb length. Also, these indices were not associated with the angiogenesis activity on the CAM. Further studies exploring the specific metabolites of the different plant parts of N. formicaria and the plant's angiopreventive potential are recommended.
{"title":"Neonauclea formicaria (Rubiaceae) Leaf Extract Inhibits Vascularization in the Chorioallantoic Membrane of Duck Embryos","authors":"J. Vergara, M. Demetillo, L. Ombat, Jashin Rosal","doi":"10.18052/www.scipress.com/ilns.83.22","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.83.22","url":null,"abstract":"Plants are reservoirs of bioactive compounds with the potential for pharmaceutical use. In this study, the secondary metabolites of Neonauclea formicaria leaf crude ethanolic extract were determined using phytochemical screening. The plant's leaf extract was then used to test its angiogenesis activity using the chorioallantoic membrane (CAM) assay. Four concentrations of the extract were prepared—0.1 mg/L, 1.0 mg/L, 10.0 mg/L, and 100.0 mg/L and were topically applied on the CAM. Phytochemical screening revealed that N. formicaria leaves contain heavy amounts of flavonoids and tannins, while alkaloids, saponins, and steroids were present in trace amounts. The crude ethanolic extract was anti-angiogenic, as indicated by the significant decrease of vascular density at higher concentrations (P<0.05). The 100 mg/L extract concentration showed the highest vascular inhibition (50.93%) among the other concentrations, suggesting its angiopreventive potential (P<0.05). Further investigation on the embryo's gross morphometry revealed no significant effects in the weight, crown-rump length, head-beak length, forelimb length, and hind limb length. Also, these indices were not associated with the angiogenesis activity on the CAM. Further studies exploring the specific metabolites of the different plant parts of N. formicaria and the plant's angiopreventive potential are recommended.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45008260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-27DOI: 10.18052/www.scipress.com/ilns.83.32
B. Usharani, N. Vasudevan
Sewage treatment through constructed wetland is an ecofriendly and sustainable approach proven effective worldwide. Constructed wetland with appropriate species is capable of eliminating all pollutants in sewage, except pathogen removal. An additional polishing treatment is required to eliminate pathogen. Optimization of HLR in CWS was executed by applying first order kinetics. Nanocomposite clay filter with economically viable materials was synthesized and disinfection ability was evaluated. A novel approach integrating constructed wetland system tailed by nanocomposite clay filter was designed. Control was setup with constructed wetland system devoid of plants integrated with clay filter devoid of nanoparticles. The constructed wetland system devoid of plants was used as plants play a vital role in the removal of pollutants. The quality of the influent for (n=20) BOD, COD, TKN, TP, TSS, TDS, SO4, Cl, lead and iron were 248, 345, 26, 4.8, 350, 450, 50, 48, 0.2, 5 mg/L respectively. The quality of effluent in the control was 145, 225, 18, 3.8, 185, 345, 31, 30, 0.6, 2 mg/L for BOD,COD, TKN, TP, TSS, TDS, SO4, Cl, lead and iron respectively. While in the test, 10, 30, 2, 1, 30, 128, 13, 12, BDL, BDL mg/L for BOD, COD, TKN, TP,TSS, TDS, SO4, Cl, lead and iron respectively. The inlet concentration of T.C, F.C and E.coli were 42.1x106-6.3x108, 4.9x105-14.4x106 and 7.8x103-3.8x105 respectively. The pathogen reduction in log removal for test and control units were 5.4 and 1.1 for T.C, 4.4 and 1.2 for F.C and 3 and 1 for E.coli. Thus it is a clean green initiative combating the limitations of disinfection surpassing the existing barriers.
{"title":"Sewage Treatment through Constructed Wetland System Tailed by Nanocomposite Clay Filter: A Clean Green Initiative","authors":"B. Usharani, N. Vasudevan","doi":"10.18052/www.scipress.com/ilns.83.32","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.83.32","url":null,"abstract":"Sewage treatment through constructed wetland is an ecofriendly and sustainable approach proven effective worldwide. Constructed wetland with appropriate species is capable of eliminating all pollutants in sewage, except pathogen removal. An additional polishing treatment is required to eliminate pathogen. Optimization of HLR in CWS was executed by applying first order kinetics. Nanocomposite clay filter with economically viable materials was synthesized and disinfection ability was evaluated. A novel approach integrating constructed wetland system tailed by nanocomposite clay filter was designed. Control was setup with constructed wetland system devoid of plants integrated with clay filter devoid of nanoparticles. The constructed wetland system devoid of plants was used as plants play a vital role in the removal of pollutants. The quality of the influent for (n=20) BOD, COD, TKN, TP, TSS, TDS, SO4, Cl, lead and iron were 248, 345, 26, 4.8, 350, 450, 50, 48, 0.2, 5 mg/L respectively. The quality of effluent in the control was 145, 225, 18, 3.8, 185, 345, 31, 30, 0.6, 2 mg/L for BOD,COD, TKN, TP, TSS, TDS, SO4, Cl, lead and iron respectively. While in the test, 10, 30, 2, 1, 30, 128, 13, 12, BDL, BDL mg/L for BOD, COD, TKN, TP,TSS, TDS, SO4, Cl, lead and iron respectively. The inlet concentration of T.C, F.C and E.coli were 42.1x106-6.3x108, 4.9x105-14.4x106 and 7.8x103-3.8x105 respectively. The pathogen reduction in log removal for test and control units were 5.4 and 1.1 for T.C, 4.4 and 1.2 for F.C and 3 and 1 for E.coli. Thus it is a clean green initiative combating the limitations of disinfection surpassing the existing barriers.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42288740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-27DOI: 10.18052/www.scipress.com/ilns.83.15
O. Nesterenko, O. Marenkov, Yehor Pylypenko
The article presents the results of studies physiology of nutrition of juveniles individuals of pumpkinseed Lepomis gibbosus (Linnaeus, 1758) from the Zaporizhzhia (Dnipro) reservoir. histological structure, morphometric parameters of digestive system and nutrition biology were investigated. The material for work were collected during scientific research in June-July 2016–2017. The ichthyological sampling was carried out in the Samara Bay of the Zaporizhzhia (Dnipro) reservoir. Researches of structure of digestive system were conducted using histological methods, researches of nutrition were conducted using ichthyological and hydrobiological methods The height of the intestinal epithelium of the pumpkinseed`s juveniles varies from 15.5 microns to 51.9 microns and on average is 32.59 ± 9.03 microns, the area of secretory goblet cells of the intestine reaches 198.61 ± 36.55 μm2. It was revealed that the pumkinseed’s juveniles only feed on phytoplankton. Research results can be useful for further study on the fish nutrition, especially sexual matured. Also, nutrition studies can help to find biomeliorative methods to combat the pumpkinseed, because the species is in competition with industrially valuable species of fish for fodder.
{"title":"Nutrition Physiology of Juveniles Individuals of Pumpkinseed Lepomis gibbosus (Linnaeus, 1758) from the Samara Bay (Ukraine)","authors":"O. Nesterenko, O. Marenkov, Yehor Pylypenko","doi":"10.18052/www.scipress.com/ilns.83.15","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.83.15","url":null,"abstract":"The article presents the results of studies physiology of nutrition of juveniles individuals of pumpkinseed Lepomis gibbosus (Linnaeus, 1758) from the Zaporizhzhia (Dnipro) reservoir. histological structure, morphometric parameters of digestive system and nutrition biology were investigated. The material for work were collected during scientific research in June-July 2016–2017. The ichthyological sampling was carried out in the Samara Bay of the Zaporizhzhia (Dnipro) reservoir. Researches of structure of digestive system were conducted using histological methods, researches of nutrition were conducted using ichthyological and hydrobiological methods The height of the intestinal epithelium of the pumpkinseed`s juveniles varies from 15.5 microns to 51.9 microns and on average is 32.59 ± 9.03 microns, the area of secretory goblet cells of the intestine reaches 198.61 ± 36.55 μm2. It was revealed that the pumkinseed’s juveniles only feed on phytoplankton. Research results can be useful for further study on the fish nutrition, especially sexual matured. Also, nutrition studies can help to find biomeliorative methods to combat the pumpkinseed, because the species is in competition with industrially valuable species of fish for fodder.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48080262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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