IP International Journal of Medical Microbiology and Tropical Diseases最新文献

{"title":"Surgical wound infection by Pluralibacter gergoviae in a diabetic patient","authors":"Srujana Prabhala, Aarthi Sundaresan, Ami Varaiya, Garvit Chitkara","doi":"10.18231/j.ijmmtd.2023.038","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2023.038","url":null,"abstract":"is reported to cause infections in immunocompromised patients and also nosocomial outbreaks. This is a case of soft tissue infection in a diabetic patient who underwent a surgery on the left breast and a bilateral reduction mammoplasty following all preliminary investigations. Post operatively, she presented with the complaints of poor wound healing and a wound gap. Immunocompromised state, uncontrolled diabetes and topical application of cream or ointment during post-operative period could be risk factors for an infection by Little is known about this organism and its clinical significance. Hence documentation of such case scenarios is essential to gather more clinically relevant information.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135485770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity of Lactobacillus acidophilus bacteriocin against clinical isolates","authors":"Ahmed Mohammed Al-Haddad, Wedad Mohammed Al-Haik, Yasser Mansour Matran, Ibrahim Mohammed Bawazir","doi":"10.18231/j.ijmmtd.2023.035","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2023.035","url":null,"abstract":"Lactic acid bacteria serve as important human probiotics, as they can produce bacteriocins from different dairy sources, and the antimicrobial activity of their bacteriocins have been documented in the scientific field. This study aimed to assess the antimicrobial activities of bacteriocin obtained from different dairy sources. An experimental analytical design was used to isolate species on de Man, Rogosa and Sharpe Media, and identify them phenotypically and evaluated their bacteriocin's antimicrobial activity against four clinical isolates. SPSS Version 22 was used for Statistical purposes. A total of 14 isolates were verified from various sample sources. These included 6 isolates from camel's milk, 2 from goat's milk, 3 from local yogurt, and 3 from Omani yogurt. The supernatants of most isolates exhibited varying levels of antimicrobial activity during the investigation. Furthermore, the action of the bacteriocin was concentration dependent. The highest level of inhibition was 14 mm for Staphylococcus aureus, 12 mm for, 12 mm for and 10 mm for when using 100μl of raw bacteriocin. Moreover, the correlation between bacteriocin activity, temperatures, and incubation times was found to be irreversible. The study reveals inherent antibacterial traits in species from local milk and milk products in Al-Shihr Town. Specifically, variant, synthesizing bacteriocin, effectively suppressed the clinical isolates, showcasing their pathogen-fighting ability. These findings offer potential for developing efficient antimicrobial methods, utilizing bacteriocin as an antibiotic in food and medicine.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135484711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of Hepatitis B and C co-infection in HIV seropositive and HIV seronegative cases in a Tertiary care hospital in Southern Haryana","authors":"Shreya Behl, Aaditya Behl, Prof. Vanita Mane, Jyoti Sangwan, P. Vohra","doi":"10.18231/j.ijmmtd.2023.019","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2023.019","url":null,"abstract":"HBV (Hepatitis B virus) and HCV (Hepatitis C virus) are the causative agents of acute as well as chronic hepatitis. Nearly, two billion people are suffering with HBV and approximately 170 million people are infected with HCV infection around the world. While patients who are infected with HIV (Human Immunodeficiency Virus) 2-4 million are found to be having chronic HBV co-infection and 4-5 million are having HCV coinfection. Due to common mode of transmission of HIV, HBV and HCV like using shared needles, syringes, other injectable devices, sexual intercourse, or even mother to baby transmission, it is common to see HBV and HCV co-infection in HIV positive individuals. This was a hospital based observational cross-sectional study. This study was conducted in Department of Microbiology, SHKM GMC, Nalhar, Nuh, Haryana. It was for one year. The sample size for HIV seropostive cases was 80 including 40 HIV positive and 40 HIV negative samples. Seroprevalence of HBV and HCV was identified on HIV positive and HIV negative samples. Seroprevalence of HBV and HCV was found to be higher in HIV positive individuals than HIV negative individuals. In HIV positive patients 10% individuals were HBsAg positive, 5% were HBeAg positive, 10% were positive by HBV RT-PCR. None of the HIV negative were coinfected with HBV. Similarly HCV-HIV coinfection was seen in 12.5% of individuals with Rapid test, ELISA and RT-PCR. None of the HIV negative were coinfected with HCV. In HIV positive individuals HBV and HCV coinfection was seen and Co-infection of HBV and HCV was absent in HIV negative individuals. Regular screening is recommended for HBV and HCV in HIV positive individuals.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74081310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, antibiotic susceptibility and plasmid profile of bacteria isolated from door handles of washrooms of a hospital in Bhopal, Madhya Pradesh","authors":"S. Shrivastava, Naveen Khare","doi":"10.18231/j.ijmmtd.2023.016","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2023.016","url":null,"abstract":"This study was conducted to determine the bacterial contamination in door handles of washrooms of a hospital, to determine the prevalence and antibiotic susceptibility pattern of the isolates. Plasmid profile was done to observe the presence or absence of plasmid among isolated bacteria from door handles of washrooms of a hospital.Washrooms of four different wards of a hospital were included in this study. Manual of Systematic Bacteriology and antibiotic susceptibility test was done by Kirby-Bauer method. Plasmid extraction was done according to modified hot alkaline method and staining technique and biochemixcal testing.Out of the 16 samples processed, 16 (100%) of them showed bacterial growth. A total of forty three (43) isolates were obtained. The bacteria isolated were spp. (37.21%), spp.(18.6%), .(16.28%), Fecal Coliform (13.95%), Micrococcus spp.(6.98%), spp.(4.65%), spp.(2.33%).Plasmid profiling revealed (11 out of 43) bacterial isolates contained 1 or more plasmids with different profiles.. Among the 43 isolates, 83.72% were found resistant to more than two antibiotics. Highest resistance percentage of the isolates was observed against Penicillin G (95.35%) followed by SXT (74.42%) and amoxicillin (65.12%), rifampicin (55.81%), tetracycline (18.60%), ciprofloxacin (23.26%), chloramphenicol (4.65%), gentamycin, (2.33%) and streptomycin (6.98%).Findings of this study indicate the presence of bacterial strains resistant to more than two antibiotics in door handles of washrooms of a hospital which can serve as potential source of diseases.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84762011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Empyema and haemothorax due to ruptured pulmonary hydatid cyst- A rare presentation","authors":"A. Marathe, M. Tadvi, Vaidehi J. Mehta","doi":"10.18231/j.ijmmtd.2023.024","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2023.024","url":null,"abstract":"Hydatidosis is a parasitic infection caused by Echinococcus granulosus (Dog Tape-worm). India is endemic for hydatid disease. The lung is the second most frequently affected organ by hydatid cysts after the liver. Hydatid cysts are mostly seen in the right lower lobe of the lung. We report an unusual presentation of ruptured pulmonary hydatid cyst as empyema and haemothorax in a 24-year-old boy who presented with dyspnoea on exertion with right-sided chest pain. Pleural fluid was suggestive of empyema. The pleural fluid examination revealed plenty of neutrophils and the presence of hooklets of hydatid cyst. Ruptured hydatid cyst is clinically and radiologically confused with many diseases, especially lung cancer. Surgical removal of hydatid cyst is accepted as primary treatment. Thoracotomy was done to excise hydatid cyst. Albendazole was given post-surgery. The patient was discharged on postoperative day 6 with good respiratory capacity. During two months follow up the patient remained asymptomatic.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87117749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symptomatic prevalence of covid-19 in vaccinated and non-vaccinated population","authors":"Jayesh Pandya, Nirali Milind Shethia, Divya Bangera, S. Saxena","doi":"10.18231/j.ijmmtd.2023.021","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2023.021","url":null,"abstract":"Coronavirus disease (COVID-19) is an infectious disease caused by the SARS-CoV-2 virus. The world has witnessed havoc with the rapid emergence of COVID-19. The unpreparedness towards the pandemic caused the whole world to face severe consequences. Emerging economies like India with a population of more than 1.3 billion faced heavy toll of life due to the rapid spread of highly contagious COVID-19 and shortage of medical supplies and infrastructure. The testing, tracing, and isolation of COVID-19 positive patients helped greatly to break the chain of COVID-19 infection, this later boosted by the vaccine production and administration on a war scale by Government of India.In this study, we have tried to understand the symptomatic effects of COVID-19 on Indian population, its prevalence, and significance. Many individuals developed symptoms like COVID-19 but tested negative for the same and vice versa. The cases of individuals not developing symptoms of COVID-19 but infected by COVID-19 act as a carrier of the virus possibly infecting other individuals unknowingly. These individuals may have weaker immunity and more submissive health conditions due to age, under surgical treatment or are infected by other chronic or acute diseases. This makes it essential to correlate and study the symptomatic incidence of COVID-19 with respect to age and vaccination status of Indian population. This study was undertaken to determine the symptomatic prevalence of COVID-19 in vaccinated and non-vaccinated patients that were tested in our laboratory. There were 86,761 samples tested out of which 49,371 samples were positives and 37,390 were negative. The samples from different parts of India were tested between1st July 2020 and 14th November 2022, dividing the total period in 5 phases for better correlation as vaccine administration started during early 2021 primarily for health workers and till it reached common people by mid of July 2021. The observations and statistical study have described the significance of vaccination and have given better insights about the working class of individuals between 18 and 45 years of age which were more exposed to infection. The study also identifies various parameters which aggravated and conciliated the symptoms of COVID-19. The study helps us to identify the prevalence and incidence of COVID-19 virus in Indian Population and can be beneficial in devising a better strategies in future to tackle symptomatic adversaries of any infection.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91474177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streptomyces tetracycline’s computational behavior against polyketide synthase of aflatoxigenic fungi","authors":"A. Kumari, Medha Jyoti, Maneesh Kumar, Ratnesh Kumar","doi":"10.18231/j.ijmmtd.2023.017","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2023.017","url":null,"abstract":"produces a variety of bioactive compounds that prevent fungal growth, including aflatoxins. Aflatoxigenic fungi ( and ) are being researched concerning spp. and can prevent the spread of aflatoxins-producing fungi. Aflatoxin-degrading enzymes, which can convert poisonous aflatoxins into less dangerous compounds, are also produced by spp. The processes through which these microorganisms can be used to reduce aflatoxins in food and agricultural systems are still the subject of active research. To evaluate the novelty of tetracycline against the biosynthesis of aflatoxin in aflatoxigenic fungi via computational approach. In this study, we performed molecular docking of polyketide synthase (Pks-A), an enzyme that initiates aflatoxin biosynthesis using tetracycline, using the online SeamDock server. Our results showed that tetracycline had a strong affinity for Pks-A in the binding pocket. The binding energy of tetracycline was -12.7 kcal/mol, indicating a strong binding affinity between the two molecules. Furthermore, the binding site was located in the active site, which is a conserved region in Pks-A and is essential for catalysing the formation of aflatoxin. The results of our docking study suggest that tetracycline may be an effective inhibitor of aflatoxin biosynthesis.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76157762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Schizophyllum commune – A rare isolate from fungal sinusitis","authors":"Viji Muthiah, Malathi Murugesan","doi":"10.18231/j.ijmmtd.2023.025","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2023.025","url":null,"abstract":"is a basidiomycete that has been occasionally reported to cause sinusitis, allergic bronchopulmonary mycosis, and co-infection with allergic bronchopulmonary aspergillosis. Even though it is an environmental fungus, cases have been reported with vascular and tissue invasion and even presented as brain abscess in immunocompromised individuals. As there are reports of as an emerging fungal pathogen causing rhinosinusitis, laboratories should not ignore this as an environmental contaminant. Microbiologists must consider this as a possible pathogen and should report with clinical correlation. We present a case report of fungal sinus infection, as it is a rare occurrence and since we have noticed it for the first time in our hospital.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80304344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of second (delta) wave and third (omicron) wave of Covid -19 based on positivity rates at a sub-district hospital in Goa","authors":"Pradnya Naik, A. Jose","doi":"10.18231/j.ijmmtd.2023.020","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2023.020","url":null,"abstract":"The covid-19 outbreak was due to a virus which emerged in china at the end of December 2019, and was widespread in more than 200 countries worldwide. In India, the virus was introduced first by travelers returning home from various countries followed by local transmission. The SARS-CoV-2 variants B.1.1.7 (Alpha), B.1.617.2 (Delta), and B.1.1.529 (Omicron) caused rapid increase of infections worldwide. A retrospective study was carried out in a sub district hospital of south Goa during second covid wave of delta variant and third covid wave of omicron variant. Throat and nasopharngeal swabs were collected in flu OPD and sent to covid lab for RTPCR by truenat and rapid antigen tests. The positivity rates were calculated and data was used to find out various differences observed in both the waves. The peak positivity rate was 61% in mid april during delta wave and 66% in mid January during omicron wave. Signs and symptoms of fever, shortness of breadth/difficult in breathing, sore throat, cough and fatigue were seen along with minor symptoms such as malaise, headache, loss of sense of smell and taste, nausea/vomiting and diarrhea. Males were more affected than females. Rate of infection were less in immunised individuals. Adults and youth were affected more in number as compared to elderly and children. In both the waves, travellers going outside state showed low positivity.The arm of the sea variant confirmed to pose a important health burden to the society on account of allure pulmonary belongings and reduced immunization coverage all along the epidemic. Due to the communicable character of two together waves, Covid experiment far surpassed the capacity of workshops to process sample books, procrastinating newsgathering and situation. The study climaxes the burden of the pandemic on states and thus the significance of voters following COVID-19 contracts for fear that this transmission.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83286671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beta-D-glucan assay as a tool for antifungal stewardship","authors":"P. Thakkar, T. Singhal, Sweta Shah, S. Ladi","doi":"10.18231/j.ijmmtd.2023.026","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2023.026","url":null,"abstract":"","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87952794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}