Pub Date : 2021-01-01DOI: 10.22034/IJPS.2021.245261
M. Mehdizadeh, Maria Tavakoli-Ardakani, Shayan Zamani, G. Zamani, N. Nikpour
Hematopoietic stem cell transplantation (HSCT) is an effective treatment for many hematological malignancies. Engraftment is the foremost step in the autologous hematopoietic stem cell transplantation (AHSCT) process in which different granulocyte colony-stimulating factors with various administration are used. In this study, we evaluated and compared the efficacy and side effects of two forms of recombinant granulocyte colony-stimulating growth factors (GCSF) ,GCSF and Peg GCSF, In this randomized clinical trial, 60 consecutive patients with multiple myeloma, Hodgkin's and non-Hodgkin's lymphoma who underwent AHSCT were included, the average age of the patients was 55; the patients were then divided into two groups so the comparison of efficacy and side effects between the two methods become achievable. The local ethical committee approved the study with the code of SB2019:210291, and the Helsinki declaration was respected across the study. In the first group, patients received peg GCSF at a dose of 6 mg on day five, and the second group received GCSF started with 5μg/kg from day 5. We compared engraftment time and adverse effects in the two groups. Our study demonstrated no difference between the two groups regarding need for transfusion and infection complications; also, the two groups did not differ in terms of the flue-like syndrome, the type of infection and the recorded number of febrile neutropenia. Mean leukocyte engraftment days were 10.97 ± 1 and 11.1 ± 1.1 that was similar in both groups (P=0.328). Mean platelet engraftment days were 11.03 ± 2.4 and 11.1 ± 2.4 without significant difference (P=0.714).it was concluded that Pegfilgrastimhas the same efficacy and safety profile in comparison to Filgrastim. Therefore, since Pegfilgrastim has a easier method of injection and can simplify the HSCT process.
{"title":"Evaluation of Engraftment and Adverse Effects of Granulocyte Colony Stimulating Factor versus PEG Granulocyte Colony Stimulating Factor in Patients Undergoing Autologous Hematopoietic Stem Cell Transplantation","authors":"M. Mehdizadeh, Maria Tavakoli-Ardakani, Shayan Zamani, G. Zamani, N. Nikpour","doi":"10.22034/IJPS.2021.245261","DOIUrl":"https://doi.org/10.22034/IJPS.2021.245261","url":null,"abstract":"Hematopoietic stem cell transplantation (HSCT) is an effective treatment for many hematological malignancies. Engraftment is the foremost step in the autologous hematopoietic stem cell transplantation (AHSCT) process in which different granulocyte colony-stimulating factors with various administration are used. In this study, we evaluated and compared the efficacy and side effects of two forms of recombinant granulocyte colony-stimulating growth factors (GCSF) ,GCSF and Peg GCSF, In this randomized clinical trial, 60 consecutive patients with multiple myeloma, Hodgkin's and non-Hodgkin's lymphoma who underwent AHSCT were included, the average age of the patients was 55; the patients were then divided into two groups so the comparison of efficacy and side effects between the two methods become achievable. The local ethical committee approved the study with the code of SB2019:210291, and the Helsinki declaration was respected across the study. In the first group, patients received peg GCSF at a dose of 6 mg on day five, and the second group received GCSF started with 5μg/kg from day 5. We compared engraftment time and adverse effects in the two groups. Our study demonstrated no difference between the two groups regarding need for transfusion and infection complications; also, the two groups did not differ in terms of the flue-like syndrome, the type of infection and the recorded number of febrile neutropenia. Mean leukocyte engraftment days were 10.97 ± 1 and 11.1 ± 1.1 that was similar in both groups (P=0.328). Mean platelet engraftment days were 11.03 ± 2.4 and 11.1 ± 2.4 without significant difference (P=0.714).it was concluded that Pegfilgrastimhas the same efficacy and safety profile in comparison to Filgrastim. Therefore, since Pegfilgrastim has a easier method of injection and can simplify the HSCT process.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"68 1","pages":"99-106"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68029324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.22034/IJPS.2020.124282.1656
A. Taleghani, R. Akbari
Pomegranate (Punica granatum L.) is one of the oldest known fruits native to Iran. In addition to multiple studies carried out on different parts of pomegranate, the peels are noted due to various phytochemicals in different colors and regions. In the present study, major anthocyanins and non-anthocyanins of an Iranian black pomegranate cultivar (i.e., pomegranate samples of Ghaemshahr, Iran) were analyzed using high-performance liquid chromatography-mass spectrometry. In total, 30 compounds were obtained from pomegranate peels. Among these compounds, 5 anthocyanins, 1 gallotannin, 15 ellagitannins, 4 hydroxybenzoic acids, 2 gallagyl esters, 1 dihydroflavonol, and 7 hydroxycinnamic acids were identified based on their fragmentation patterns and ultraviolet spectra. Among the varieties of Iranian pomegranates, the anthocyanins of peonidin-hexoside and cyanidin-pentoside were identified in this species for the first time. Peonidin-hexoside, caffeic acid, coumaric acid-hexoside, and galloyl-hexoside were major phenolic compounds. In addition, the antioxidant activity of different fractions was tested using DPPH free radical scavenging in vitro assay (IC50: 133.13-557.23 µg/mL). The obtained results of this study highlighted that this cultivar can be an important candidate for future new drug discoveries.
{"title":"Diversity of Phenolic Profiles in Peel of an Iranian Pomegranate Cultivar (Punica granatum L.)","authors":"A. Taleghani, R. Akbari","doi":"10.22034/IJPS.2020.124282.1656","DOIUrl":"https://doi.org/10.22034/IJPS.2020.124282.1656","url":null,"abstract":"Pomegranate (Punica granatum L.) is one of the oldest known fruits native to Iran. In addition to multiple studies carried out on different parts of pomegranate, the peels are noted due to various phytochemicals in different colors and regions. In the present study, major anthocyanins and non-anthocyanins of an Iranian black pomegranate cultivar (i.e., pomegranate samples of Ghaemshahr, Iran) were analyzed using high-performance liquid chromatography-mass spectrometry. In total, 30 compounds were obtained from pomegranate peels. Among these compounds, 5 anthocyanins, 1 gallotannin, 15 ellagitannins, 4 hydroxybenzoic acids, 2 gallagyl esters, 1 dihydroflavonol, and 7 hydroxycinnamic acids were identified based on their fragmentation patterns and ultraviolet spectra. Among the varieties of Iranian pomegranates, the anthocyanins of peonidin-hexoside and cyanidin-pentoside were identified in this species for the first time. Peonidin-hexoside, caffeic acid, coumaric acid-hexoside, and galloyl-hexoside were major phenolic compounds. In addition, the antioxidant activity of different fractions was tested using DPPH free radical scavenging in vitro assay (IC50: 133.13-557.23 µg/mL). The obtained results of this study highlighted that this cultivar can be an important candidate for future new drug discoveries.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"17 1","pages":"51-68"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68028409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.22034/IJPS.2020.115048.1612
M. Nooranian, Sakineh Saghaeiannejad Isfahani, Hoda Memarzadeh
Information management is considered as one of the keys to the supply chain management (SCM) success. This study intends to explore the factors affecting the knowledge sharing and knowledge quality in the SCM of the drugs and medical consumables. This applied study was conducted using analytical-survey research method. The research population consisted of 78 people involved in the supply chain of the drug and medical consumables in the teaching hospitals of Isfahan University of Medical Sciences (IUMS). Data were collected by a questionnaire whose face and content validity were both confirmed by pooling the ideas of some professors of Health Information Technology and Health-care Services Management faculties of IUMS. The reliability of the questionnaire was validated by estimating Cornbrash’s alpha coefficient (α=0.93). Data were analyzed using multiple regression analysis method and SPSS software version.23. Based on the results, the environmental uncertainty and intra-organizational facilitators had no impact on information sharing and information quality but inter-organizational relationships influenced the information sharing (p = 0.01) and information quality (p = 0.001). To achieve high-quality information sharing, establishing appropriate relationships with the other chain partners is of critical importance. Based on the results of the study, although there was no statistically significant relationship between the intra-organizational facilitators and environmental uncertainty and the information sharing and information quality, top management support, information technology (IT), appropriate selection of supplier and attention to the drug needs of the patients, should not be neglected.
{"title":"Information Sharing and Information Quality in the Drugs and Medical Consumables Supply Chain Management (SCM)","authors":"M. Nooranian, Sakineh Saghaeiannejad Isfahani, Hoda Memarzadeh","doi":"10.22034/IJPS.2020.115048.1612","DOIUrl":"https://doi.org/10.22034/IJPS.2020.115048.1612","url":null,"abstract":"Information management is considered as one of the keys to the supply chain management (SCM) success. This study intends to explore the factors affecting the knowledge sharing and knowledge quality in the SCM of the drugs and medical consumables. This applied study was conducted using analytical-survey research method. The research population consisted of 78 people involved in the supply chain of the drug and medical consumables in the teaching hospitals of Isfahan University of Medical Sciences (IUMS). Data were collected by a questionnaire whose face and content validity were both confirmed by pooling the ideas of some professors of Health Information Technology and Health-care Services Management faculties of IUMS. The reliability of the questionnaire was validated by estimating Cornbrash’s alpha coefficient (α=0.93). Data were analyzed using multiple regression analysis method and SPSS software version.23. Based on the results, the environmental uncertainty and intra-organizational facilitators had no impact on information sharing and information quality but inter-organizational relationships influenced the information sharing (p = 0.01) and information quality (p = 0.001). To achieve high-quality information sharing, establishing appropriate relationships with the other chain partners is of critical importance. Based on the results of the study, although there was no statistically significant relationship between the intra-organizational facilitators and environmental uncertainty and the information sharing and information quality, top management support, information technology (IT), appropriate selection of supplier and attention to the drug needs of the patients, should not be neglected.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"17 1","pages":"27-42"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68027933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.22034/IJPS.2020.128484.1673
A. Salimi, M. Shirani
Background: vulvovaginal candidiasis (VVC) caused by Candida albicans and other Candida species are high-risk event in patients admitted to hospital. Ziziphus genus is a rich source of medicinal compounds, effective for the treatment or prevention of some diseases. Objectives: This study aimed to evaluate the antifungal effect of topical microemulsion containing Ziziphus spina-christi L (ZSC) extracts against Candida spp.Methods: Z. Spina-Christi was collected and extracted in a Soxhlet apparatus using the ethanol and methanol solvents. Microemulsions were formulated by varying the composition of surfactant (Tween 80) along with cosurfactant (propylene glycol), oil, and water and were characterized regarding their stability. The microemulsion without extract as placebo and the active microemulsion containing ZSC extract as active formulation were applied to test the antifungal activity against C. albicans, C. parapsilosis and C. krusei using well diffusion test. The optimal microemulsion was selected and then analyzed for average diameter of droplets, pH, viscosity and zeta potential.Results: The microemulsion containing ZSC ethanolic extract had a significant inhibitory effect on the different species of Candida, but the most inhibitory effect was found against C. albicans. Conclusion: The results of this study indicate that the ethanol extract has interesting antimicrobial properties and can used for the treatment of fungal infections. More research is required to check this plant performance to treat the Candidiasis.
{"title":"Antifungal Activity of Topical Microemulsion Containing Ziziphus spina-christi L. for the Treatment of Fungal Vaginitis","authors":"A. Salimi, M. Shirani","doi":"10.22034/IJPS.2020.128484.1673","DOIUrl":"https://doi.org/10.22034/IJPS.2020.128484.1673","url":null,"abstract":"Background: vulvovaginal candidiasis (VVC) caused by Candida albicans and other Candida species are high-risk event in patients admitted to hospital. Ziziphus genus is a rich source of medicinal compounds, effective for the treatment or prevention of some diseases. Objectives: This study aimed to evaluate the antifungal effect of topical microemulsion containing Ziziphus spina-christi L (ZSC) extracts against Candida spp.Methods: Z. Spina-Christi was collected and extracted in a Soxhlet apparatus using the ethanol and methanol solvents. Microemulsions were formulated by varying the composition of surfactant (Tween 80) along with cosurfactant (propylene glycol), oil, and water and were characterized regarding their stability. The microemulsion without extract as placebo and the active microemulsion containing ZSC extract as active formulation were applied to test the antifungal activity against C. albicans, C. parapsilosis and C. krusei using well diffusion test. The optimal microemulsion was selected and then analyzed for average diameter of droplets, pH, viscosity and zeta potential.Results: The microemulsion containing ZSC ethanolic extract had a significant inhibitory effect on the different species of Candida, but the most inhibitory effect was found against C. albicans. Conclusion: The results of this study indicate that the ethanol extract has interesting antimicrobial properties and can used for the treatment of fungal infections. More research is required to check this plant performance to treat the Candidiasis.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"17 1","pages":"43-50"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68027947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-01DOI: 10.22034/IJPS.2019.106412.1549
Farahnaz Tanbakosazan, P. Derakhshi, P. A. Azar, K. Ashtari, P. Naserzadeh, Enayatollah Seydi, J. Pourahmad
Industrial wastewater is of global concern due to its severe effects on the environment. Compared with municipal wastewater, industrial wastewater generally contains the high concentration of toxic or no biodegradable pollutants. In recently year, scientific showed that scallop could filtration wastewater. Therefore, it was decided to determine the mechanistic toxicity of wastewater contained NPs (Ag and TiO2) towards isolated mitochondria via reliable methods. Isolated muscle scallop mitochondria were obtained by differential ultracentrifugation on before and after exposure to wastewater. Our results showed that two NPs (Ag and TiO2) induced mitochondrial dysfunction via an increase in mitochondrial reactive oxygen species (ROS) generation, lipid peroxidation (LPO) and mitochondrial membrane potential (MMP) collapse. Finally, Ag-NPs and TiO2-NPs have reduced the level of glutathione (GSH) and also induced apoptosis. Our results suggest that wastewater contained NPs -induced toxicity is the result of a disruptive effect on the mitochondrial respiratory chain, increasing the chance of cell death signaling.
{"title":"Selective Toxicity of Ag/TiO2 Nanoparticles of Waste Water of industrial factories on muscle Mitochondria Isolated from Solendactylus scallop","authors":"Farahnaz Tanbakosazan, P. Derakhshi, P. A. Azar, K. Ashtari, P. Naserzadeh, Enayatollah Seydi, J. Pourahmad","doi":"10.22034/IJPS.2019.106412.1549","DOIUrl":"https://doi.org/10.22034/IJPS.2019.106412.1549","url":null,"abstract":"Industrial wastewater is of global concern due to its severe effects on the environment. Compared with municipal wastewater, industrial wastewater generally contains the high concentration of toxic or no biodegradable pollutants. In recently year, scientific showed that scallop could filtration wastewater. Therefore, it was decided to determine the mechanistic toxicity of wastewater contained NPs (Ag and TiO2) towards isolated mitochondria via reliable methods. Isolated muscle scallop mitochondria were obtained by differential ultracentrifugation on before and after exposure to wastewater. Our results showed that two NPs (Ag and TiO2) induced mitochondrial dysfunction via an increase in mitochondrial reactive oxygen species (ROS) generation, lipid peroxidation (LPO) and mitochondrial membrane potential (MMP) collapse. Finally, Ag-NPs and TiO2-NPs have reduced the level of glutathione (GSH) and also induced apoptosis. Our results suggest that wastewater contained NPs -induced toxicity is the result of a disruptive effect on the mitochondrial respiratory chain, increasing the chance of cell death signaling.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"16 1","pages":"57-68"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44057827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-01DOI: 10.22034/IJPS.2019.102748.1524
D. Nurhidayati, V. Yurina, Eka Riza Maula, Gisselia Eurika Wijaya
Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. The α-mangostin in mangosteen pericarp extract can inhibit M. tuberculosis growth. This study examined the potency of α-mangostin in mangosteen pericarp extract to inhibit 38-kDa and Ag85 protein secretion from M. tuberculosis H37Rv. The samples used in this study were divided into three independent variable groups (3.125 µg/ml; 6.25 µg/ml; and 12.5 µg/ml α-mangostin in the mangosteen pericarp extract), a positive control group (rifampin), a negative control group (M. tuberculosis H37Rv), and the Garcia® group (trademark of the mangosteen peel extract capsule). HPLC-MS/MS detected that the mangosteen pericarp extract contained 5,984.55 µg/g α-mangostin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and dot blot analysis were conducted to analyze the profile and specificity of both proteins. This study revealed that 6.25 µg/ml of mangosteen pericarp extract had the greatest potency for inhibiting 38-kDa protein secretion from M. tuberculosis H37Rv, and 12.5 µg/ml mangosteen pericarp extract had greater potency for inhibiting Ag85 protein secretion than did the other doses. Thus, α-mangosteen from mangosteen pericarp extract can inhibit Ag38 and Ag85 secretion.
{"title":"Potency of Mangosteen Pericarp Extract to Inhibit 38-kDa and Ag85 Protein Secretion by Mycobacterium tuberculosis H37Rv","authors":"D. Nurhidayati, V. Yurina, Eka Riza Maula, Gisselia Eurika Wijaya","doi":"10.22034/IJPS.2019.102748.1524","DOIUrl":"https://doi.org/10.22034/IJPS.2019.102748.1524","url":null,"abstract":"Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. The α-mangostin in mangosteen pericarp extract can inhibit M. tuberculosis growth. This study examined the potency of α-mangostin in mangosteen pericarp extract to inhibit 38-kDa and Ag85 protein secretion from M. tuberculosis H37Rv. The samples used in this study were divided into three independent variable groups (3.125 µg/ml; 6.25 µg/ml; and 12.5 µg/ml α-mangostin in the mangosteen pericarp extract), a positive control group (rifampin), a negative control group (M. tuberculosis H37Rv), and the Garcia® group (trademark of the mangosteen peel extract capsule). HPLC-MS/MS detected that the mangosteen pericarp extract contained 5,984.55 µg/g α-mangostin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and dot blot analysis were conducted to analyze the profile and specificity of both proteins. This study revealed that 6.25 µg/ml of mangosteen pericarp extract had the greatest potency for inhibiting 38-kDa protein secretion from M. tuberculosis H37Rv, and 12.5 µg/ml mangosteen pericarp extract had greater potency for inhibiting Ag85 protein secretion than did the other doses. Thus, α-mangosteen from mangosteen pericarp extract can inhibit Ag38 and Ag85 secretion.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"16 1","pages":"19-26"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48083400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-01DOI: 10.22034/IJPS.2019.103550.1531
A. Salimi, E. Moghimipour, Payam Kogani, S. Soleymani
Octyl methoxycinnamate is one of the ingredients in sunscreen products. The main aim of this study was to investigate the effect of different enhancers of in vitro skin permeability of Octyl methoxycinnamate. Octyl methoxycinnamate permeability parameters were evaluated through the whole skin of the rat with and without chemical enhancers including eucalyptus oil, urea, menthol and olive oil by Franz cell diffusion. The effects of enhancers on skin structure were also studied using DSC and FT-IR techniques. The skin prevented the permeability of Octyl methoxycinnamate so that after 24 hours less than 3% of the substance passed through the skin. The results of this study showed that by increasing the time, it is possible to increase the skin permeation and the highest rate of skin absorption were corresponded to olive oil (ERflux=63.074), eucalyptus oil (ERflux=48.78) and menthol (ERflux=33.5), respectively while the least amount of skin absorption was related to urea (ERflux=29.53). Chemical penetration enhancers are substances that interfere with the complex structure of the skin and protein lipids. Two endothermic transitions were obtained at about 67 (Tm1) and 112 ° C (Tm2) in thermogram of the hydrated whole rat skin. Tm1 and Tm2 seems to be due to the melting of the lipids and the irreversible intracellular keratin or melting of the lipid-protein (keratin) complex, respectively. The amount of Tm1, ΔH1 and ΔH2 were decreased by all penetration enhancers compared to the hydrated skin. The FT-IR results suggested the mechanism of increasing absorption effect by lipid fluidization and lipid extraction. All of penetration enhancers used in this study significantly increased the skin permeability of Octyl methoxycinnamate.
{"title":"The Effect of Various Penetration Enhancers on the Octyl Methoxycinnamate Permeability: Mechanisms of Action Study","authors":"A. Salimi, E. Moghimipour, Payam Kogani, S. Soleymani","doi":"10.22034/IJPS.2019.103550.1531","DOIUrl":"https://doi.org/10.22034/IJPS.2019.103550.1531","url":null,"abstract":"Octyl methoxycinnamate is one of the ingredients in sunscreen products. The main aim of this study was to investigate the effect of different enhancers of in vitro skin permeability of Octyl methoxycinnamate. Octyl methoxycinnamate permeability parameters were evaluated through the whole skin of the rat with and without chemical enhancers including eucalyptus oil, urea, menthol and olive oil by Franz cell diffusion. The effects of enhancers on skin structure were also studied using DSC and FT-IR techniques. The skin prevented the permeability of Octyl methoxycinnamate so that after 24 hours less than 3% of the substance passed through the skin. The results of this study showed that by increasing the time, it is possible to increase the skin permeation and the highest rate of skin absorption were corresponded to olive oil (ERflux=63.074), eucalyptus oil (ERflux=48.78) and menthol (ERflux=33.5), respectively while the least amount of skin absorption was related to urea (ERflux=29.53). Chemical penetration enhancers are substances that interfere with the complex structure of the skin and protein lipids. Two endothermic transitions were obtained at about 67 (Tm1) and 112 ° C (Tm2) in thermogram of the hydrated whole rat skin. Tm1 and Tm2 seems to be due to the melting of the lipids and the irreversible intracellular keratin or melting of the lipid-protein (keratin) complex, respectively. The amount of Tm1, ΔH1 and ΔH2 were decreased by all penetration enhancers compared to the hydrated skin. The FT-IR results suggested the mechanism of increasing absorption effect by lipid fluidization and lipid extraction. All of penetration enhancers used in this study significantly increased the skin permeability of Octyl methoxycinnamate.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"16 1","pages":"87-104"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45502360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.22034/IJPS.2019.104391.1533
E. Darmawan, L. Kusumawati
This study aimed to examine the effectiveness of combining salbutamol nebulizer and oral procaterol compared to only oral procaterol on the respiratory rate, temperature (fever), spasm, cough frequency, thoracic retraction, and length of stay (LOS) among young children with pneumonia. This prospective cohort study included 48 consecutive sampling subjects with pneumonia in Indonesia. The subjects were classified based on the type of therapy they received: intervention group used the combination of salbutamol nebulizer and oral procaterol therapy (n=24 subjects) and the control group received oral procaterol only (n=24). The result showed that the combination therapy salbutamol nebulizer and oral procaterol was more effective than the administration of oral procaterol only with the rate of clinical change. Combination therapy nebulized salbutamol and oral procaterol significantly respiratory rate (p=0.006) and temperature (fever) (p=0.002) compared to the only oral procaterol. The combination had clinical change in reducing spasm, cough frequency, thoracic retraction, and length of stay (LOS) however there are was no significant effect of this combination in reducing spasm (p=0.348), cough frequency (p=0.964), thoracic retraction (p=0.666), and length of stay (LOS) (p=0.192) compared to the only oral procaterol among the Indonesian children with pneumonia.
{"title":"The Combination of Salbutamol Nebulizer and Oral Procaterol to the Indonesian Children with Pneumonia","authors":"E. Darmawan, L. Kusumawati","doi":"10.22034/IJPS.2019.104391.1533","DOIUrl":"https://doi.org/10.22034/IJPS.2019.104391.1533","url":null,"abstract":"This study aimed to examine the effectiveness of combining salbutamol nebulizer and oral procaterol compared to only oral procaterol on the respiratory rate, temperature (fever), spasm, cough frequency, thoracic retraction, and length of stay (LOS) among young children with pneumonia. This prospective cohort study included 48 consecutive sampling subjects with pneumonia in Indonesia. The subjects were classified based on the type of therapy they received: intervention group used the combination of salbutamol nebulizer and oral procaterol therapy (n=24 subjects) and the control group received oral procaterol only (n=24). The result showed that the combination therapy salbutamol nebulizer and oral procaterol was more effective than the administration of oral procaterol only with the rate of clinical change. Combination therapy nebulized salbutamol and oral procaterol significantly respiratory rate (p=0.006) and temperature (fever) (p=0.002) compared to the only oral procaterol. The combination had clinical change in reducing spasm, cough frequency, thoracic retraction, and length of stay (LOS) however there are was no significant effect of this combination in reducing spasm (p=0.348), cough frequency (p=0.964), thoracic retraction (p=0.666), and length of stay (LOS) (p=0.192) compared to the only oral procaterol among the Indonesian children with pneumonia.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"16 1","pages":"59-72"},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48668057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.22034/IJPS.2020.114372.1608
J. Sargolzaei, H. Sadeghian, Sanaz Golahmadi, M. Soukhtanloo
Neuroblastoma is one of the nervous system cancers, which approximately consists of 9% of childhood cancers. In this study, we evaluated the toxic effects of prenyl hydroxy coumarin derivatives on apoptosis of the neuroblastoma cell line N2A. N2a cells were cultured in DMEM medium, then the effects of different concentrations (0.75–200 μg/mL) of prenyl hydroxy coumarin derivatives during 24, 48, and 72 h were studied. Cell viability was quantified by MTT assay; apoptotic cells were determined using PI staining of DNA fragmentation by Flow cytometry (sub-G1 peak). The toxic effect of 3- farnesyl oxi coumarin in the N2A cell starts at 6.25 μg/ml and increases relatively depending on rising in concentration and time. The toxicity and apoptosis in 3- farnesyl and 6- farnesyl oxi coumarin is more than 3- Geranyl and 6-Geranyl oxi coumarin. Prenyl hydroxy coumarin induces peak sub-G1in flow cytometry compared to the control group, indicating prenyl hydroxy coumarin-induced toxicity, which is involved in apoptotic cell death. Different concentration of hydroxy coumarin derivatives (0.75-200) µg/mL in lymphocytes, did not induce any anti-proliferative effect in 24 h. In conclusion, prenyl hydroxy coumarin derivatives induce apoptotic effects in the N2A cell line. Thus prenyl hydroxy coumarin derivatives sound to be chemotherapeutic agents for the neuroblastoma cancer cells.
{"title":"Cytotoxic effects of hydroxy coumarin derivations to neuroblastoma N2a cells","authors":"J. Sargolzaei, H. Sadeghian, Sanaz Golahmadi, M. Soukhtanloo","doi":"10.22034/IJPS.2020.114372.1608","DOIUrl":"https://doi.org/10.22034/IJPS.2020.114372.1608","url":null,"abstract":"Neuroblastoma is one of the nervous system cancers, which approximately consists of 9% of childhood cancers. In this study, we evaluated the toxic effects of prenyl hydroxy coumarin derivatives on apoptosis of the neuroblastoma cell line N2A. N2a cells were cultured in DMEM medium, then the effects of different concentrations (0.75–200 μg/mL) of prenyl hydroxy coumarin derivatives during 24, 48, and 72 h were studied. Cell viability was quantified by MTT assay; apoptotic cells were determined using PI staining of DNA fragmentation by Flow cytometry (sub-G1 peak). The toxic effect of 3- farnesyl oxi coumarin in the N2A cell starts at 6.25 μg/ml and increases relatively depending on rising in concentration and time. The toxicity and apoptosis in 3- farnesyl and 6- farnesyl oxi coumarin is more than 3- Geranyl and 6-Geranyl oxi coumarin. Prenyl hydroxy coumarin induces peak sub-G1in flow cytometry compared to the control group, indicating prenyl hydroxy coumarin-induced toxicity, which is involved in apoptotic cell death. Different concentration of hydroxy coumarin derivatives (0.75-200) µg/mL in lymphocytes, did not induce any anti-proliferative effect in 24 h. In conclusion, prenyl hydroxy coumarin derivatives induce apoptotic effects in the N2A cell line. Thus prenyl hydroxy coumarin derivatives sound to be chemotherapeutic agents for the neuroblastoma cancer cells.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"16 1","pages":"95-106"},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46378384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.22034/IJPS.2019.112151.1595
M. Najafzadeh, S. Sajjadi, A. Z. Moghaddam, M. Fereidouni
Silymarin is a safe herbal medicine; however, it has some undesirable properties such as short half-life and poor aqueous solubility. To the best of our knowledge, this study is the first to report utilizing a dual-drug delivery system (DDDS) to enhance the release profile of silymarin from both micelles and hydrogels. In this experimental study, the release profile of micellar silymarin and micelle-hydrogel bounded silymarin during 21 days was examined using Knauer K2600A liquid chromatography. The calibration curve was plotted using the peak-areas of the silymarin at different concentrations. The RP-C18 column allowed a good separation of the components of standard silymarin. LOD and LOQ were 16.5 and 55.02 μg/ml, respectively. The in vitro release profiles of the two compounds showed a rapid release of silymarin, especially in the absence of hydrogel. The cumulative release graph revealed that the hydrogel-bound form has more constant release kinetics than the free micelle form; this means that the hydrogel-bound form may sustain for longer durations. In this study, a dual-drug delivery system based on hydrogel/micelle composites was introduced. The results showed that Puramatrix hydrogel plays an important role in the constant release of silymarin. Furthermore, the RP-HPLC method presented in this study can be used by other researchers to overcome the difficulties associated with the in-vitro separation and quantification of silymarin.
{"title":"The study of silymarin release kinetic in free and hydrogel bound micellar forms: a qualitative and quantitative analysis using RP-HPLC","authors":"M. Najafzadeh, S. Sajjadi, A. Z. Moghaddam, M. Fereidouni","doi":"10.22034/IJPS.2019.112151.1595","DOIUrl":"https://doi.org/10.22034/IJPS.2019.112151.1595","url":null,"abstract":"Silymarin is a safe herbal medicine; however, it has some undesirable properties such as short half-life and poor aqueous solubility. To the best of our knowledge, this study is the first to report utilizing a dual-drug delivery system (DDDS) to enhance the release profile of silymarin from both micelles and hydrogels. In this experimental study, the release profile of micellar silymarin and micelle-hydrogel bounded silymarin during 21 days was examined using Knauer K2600A liquid chromatography. The calibration curve was plotted using the peak-areas of the silymarin at different concentrations. The RP-C18 column allowed a good separation of the components of standard silymarin. LOD and LOQ were 16.5 and 55.02 μg/ml, respectively. The in vitro release profiles of the two compounds showed a rapid release of silymarin, especially in the absence of hydrogel. The cumulative release graph revealed that the hydrogel-bound form has more constant release kinetics than the free micelle form; this means that the hydrogel-bound form may sustain for longer durations. In this study, a dual-drug delivery system based on hydrogel/micelle composites was introduced. The results showed that Puramatrix hydrogel plays an important role in the constant release of silymarin. Furthermore, the RP-HPLC method presented in this study can be used by other researchers to overcome the difficulties associated with the in-vitro separation and quantification of silymarin.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"16 1","pages":"73-80"},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47987880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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