Pub Date : 2018-01-01DOI: 10.22034/IJPS.2018.32052
Y. Shakiba, S. E. Rezatofighi, S. M. Seyyednejad, M. Ardakani
Foot-and-mouth disease (FMD) is a major infectious disease of cloven-hoofed animals that is caused by the FMD virus (FMDV). This disease has significantly adverse economic impacts; therefore, rapid control measures are urgently. Traditional ranchers in Iran use Alhagi maurorum Medik. to treat FMD; therefore, we aimed to examine the antiviral activity of methanolic, ethanolic, and aqueous-acetic acid extracts of this plant against FMDV. The cytotoxicity of the extracts was assayed by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) method and the antiviral activity of the extracts was evaluated by measuring the percentage of viable FMDV infected-cells via the MTT assay at different stages of the virus replication cycle. The results indicated that the plant extracts exhibit antiviral activity against FMDV at all stages of the experiment, although the most significant effects were observed in virucidal and pre-treatment assays. These findings represent the anti-FMDV activities of A. maurorum extracts at several stages of the virus replication cycle; therefore, it could be considered for the potential development of anti-FMDV therapeutics. This creates the basis for further analyses of the molecular mechanisms of the antiviral activity of this herbal plant.
{"title":"Inhibition of Foot-and-Mouth Disease Virus Replication by Hydro-alcoholic and Aqueous-Acetic Acid Extracts of Alhagi maurorum","authors":"Y. Shakiba, S. E. Rezatofighi, S. M. Seyyednejad, M. Ardakani","doi":"10.22034/IJPS.2018.32052","DOIUrl":"https://doi.org/10.22034/IJPS.2018.32052","url":null,"abstract":"Foot-and-mouth disease (FMD) is a major infectious disease of cloven-hoofed animals that is caused by the FMD virus (FMDV). This disease has significantly adverse economic impacts; therefore, rapid control measures are urgently. Traditional ranchers in Iran use Alhagi maurorum Medik. to treat FMD; therefore, we aimed to examine the antiviral activity of methanolic, ethanolic, and aqueous-acetic acid extracts of this plant against FMDV. The cytotoxicity of the extracts was assayed by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) method and the antiviral activity of the extracts was evaluated by measuring the percentage of viable FMDV infected-cells via the MTT assay at different stages of the virus replication cycle. The results indicated that the plant extracts exhibit antiviral activity against FMDV at all stages of the experiment, although the most significant effects were observed in virucidal and pre-treatment assays. These findings represent the anti-FMDV activities of A. maurorum extracts at several stages of the virus replication cycle; therefore, it could be considered for the potential development of anti-FMDV therapeutics. This creates the basis for further analyses of the molecular mechanisms of the antiviral activity of this herbal plant.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"14 1","pages":"85-96"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68024399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-01DOI: 10.22034/ijps.2017.31131
V. Rajurkar, Sagar M. Shirsath
The green synthesis of 5-(4-aminophenyl)-4-aryl-4H-1,2,4-triazole-3-thiol was achieved in four steps, In first step, 4-amino benzoic acid refluxed in ethanol along with catalyst Conc. Sulphuric acid to produce ethyl-4-amino benzoate I. Further compound I refluxed with hydrazine hydrate in ethanol to produce 4-amino benzohydrazide II. Compound II refluxed in ethanolic potassium hydroxide with carbon disulfide to produce 5-(4-aminophenyl)-1,3,4-oxadiazole-2-thiol III. Compound III refluxed in ethanol with different substituted primary aryl amine gave title compounds 5-(4-aminophenyl)-4-aryl-4H-1,2,4-triazole-3-thiol IVa-o. The compounds obtained were identified by FT-IR, 1H-NMR, GC- mass spectroscopy data, and elemental analysis and also screened for in-vivo antimicrobial activity. In vitro antibacterial activity was carried out against organisms like E.coli. K.pneumonia, S.aureus, and B.subtilis as well as in vitro antifungal activity were carried out against organisms like A.niger and S.cerevisiae. In vitro antimicrobial evaluation, the most potent broad spectrum compound IVc, IVd and IVf were found significant agent against standard drug Norfloxacin and Ketoconazole.
{"title":"Green synthesis and evaluation of 5-(4-aminophenyl)-4-aryl-4H-1, 2, 4-triazole-3-thiol derivatives","authors":"V. Rajurkar, Sagar M. Shirsath","doi":"10.22034/ijps.2017.31131","DOIUrl":"https://doi.org/10.22034/ijps.2017.31131","url":null,"abstract":"The green synthesis of 5-(4-aminophenyl)-4-aryl-4H-1,2,4-triazole-3-thiol was achieved in four steps, In first step, 4-amino benzoic acid refluxed in ethanol along with catalyst Conc. Sulphuric acid to produce ethyl-4-amino benzoate I. Further compound I refluxed with hydrazine hydrate in ethanol to produce 4-amino benzohydrazide II. Compound II refluxed in ethanolic potassium hydroxide with carbon disulfide to produce 5-(4-aminophenyl)-1,3,4-oxadiazole-2-thiol III. Compound III refluxed in ethanol with different substituted primary aryl amine gave title compounds 5-(4-aminophenyl)-4-aryl-4H-1,2,4-triazole-3-thiol IVa-o. The compounds obtained were identified by FT-IR, 1H-NMR, GC- mass spectroscopy data, and elemental analysis and also screened for in-vivo antimicrobial activity. In vitro antibacterial activity was carried out against organisms like E.coli. K.pneumonia, S.aureus, and B.subtilis as well as in vitro antifungal activity were carried out against organisms like A.niger and S.cerevisiae. In vitro antimicrobial evaluation, the most potent broad spectrum compound IVc, IVd and IVf were found significant agent against standard drug Norfloxacin and Ketoconazole.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"13 1","pages":"37-50"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44889023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-01DOI: 10.22034/IJPS.2017.31129
Mostafa Notarki, M. Setorki, Z. Hooshmandi
Medicinal plants have attracted global attention due to their high levels of antioxidant components for prohibiting or improving various diseases including brain and memory disorders. This study was designed to investigate if the antioxidant effect of Sidr (Zizyphusspina-christi) extract can help to improve the scopolamine induced impairment of memory in Wistar rats. Animals were randomly divided into 6 groups: Control, Scopolamine at 1 mg/kg, Scopolamine+50 mg/kg Sidr extract (Zsc50+sco), Scopolamine+100 mg/kg Sidr extract (Zsc100+sco), Scopolamine+200 mg/kg Sidr extract (Zsc200+sco) and Scopolamine+diazepam at 1 mg/kgbw (D+sco). Antioxidant activity and total phenolic content of Z. spina-christi as well as the Passive avoidance test, antioxidant capacity and MDA level of plasma and brain were evaluated.Scopolamine increased the recorded initial latencies but decrease the step-through latency in comparison with those of control group. The use of Sidr at 50, 100 and 200 mg/kg attenuate these abnormalities and the impaired passive avoidance memory was improved. Although scopolamine didn’t cause any change in the antioxidant capacity plasma and brain, it increased the MDA level of plasma and brain tissues. Also use of Sidr increased the antioxidant capacity and decreased the MDA level of plasma and brain tissues.The results suggest that Z. SC might act to attenuate scopolamine induced brain and memory impairments due to its high antioxidant contents. The separation of the specific compound responsible for observed antioxidant activity with protective effect against brain and memory disorders still need further researches.
{"title":"The antioxidant effect of Sidr (Zizyphusspina-christi) leaf extract helping to improve thescopolamine induced memory impairment in male rats","authors":"Mostafa Notarki, M. Setorki, Z. Hooshmandi","doi":"10.22034/IJPS.2017.31129","DOIUrl":"https://doi.org/10.22034/IJPS.2017.31129","url":null,"abstract":"Medicinal plants have attracted global attention due to their high levels of antioxidant components for prohibiting or improving various diseases including brain and memory disorders. This study was designed to investigate if the antioxidant effect of Sidr (Zizyphusspina-christi) extract can help to improve the scopolamine induced impairment of memory in Wistar rats. Animals were randomly divided into 6 groups: Control, Scopolamine at 1 mg/kg, Scopolamine+50 mg/kg Sidr extract (Zsc50+sco), Scopolamine+100 mg/kg Sidr extract (Zsc100+sco), Scopolamine+200 mg/kg Sidr extract (Zsc200+sco) and Scopolamine+diazepam at 1 mg/kgbw (D+sco). Antioxidant activity and total phenolic content of Z. spina-christi as well as the Passive avoidance test, antioxidant capacity and MDA level of plasma and brain were evaluated.Scopolamine increased the recorded initial latencies but decrease the step-through latency in comparison with those of control group. The use of Sidr at 50, 100 and 200 mg/kg attenuate these abnormalities and the impaired passive avoidance memory was improved. Although scopolamine didn’t cause any change in the antioxidant capacity plasma and brain, it increased the MDA level of plasma and brain tissues. Also use of Sidr increased the antioxidant capacity and decreased the MDA level of plasma and brain tissues.The results suggest that Z. SC might act to attenuate scopolamine induced brain and memory impairments due to its high antioxidant contents. The separation of the specific compound responsible for observed antioxidant activity with protective effect against brain and memory disorders still need further researches.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"13 1","pages":"13-24"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47075606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-01DOI: 10.22034/ijps.2017.31107
S. Nasoohi, P. Naserzadeh, Nafiseh Rahmanabadi, Banafsheh Saiid, Jalal Pourahmad Jaktaji
Background: Although the bio kinetics, metabolism and chemical toxicity of Atorvastatin are well known, until recently little attention was paid to the potential neurotoxic effect of Atorvastatin (Atv). Regarding the concrete evidences indicating Atv may reduce Coenzyme Q10 (CoQ10) levels through blockage of metalonate cycle, the present work aims to determine if Atorvastatin may provide toxic effects on brain mitochondria and whether its supplementation with two lipidicantioxidants, CoQ10 and Vit E may improve such outcomes. Methods: to evaluate mitochondrial toxicity, male NMRI mice were first treated with Atorvastatin(bo; 20 and 60 mg/kg) every other day with or without supplementation with CoQ10 (200 mg/kg) or Vit E (40 u/kg). After a period of 4 weeks, the animals were euthanized and brain cortices were harvested ad subjected to mitochondria isolation procedure. ROS release, mitochondrial membrane potential (MMP) and cytochrome c release were performed to precisely address the probable mitochondrial injury. Findings: Our data showed increased ROS formation, MMP collapse, mitochondrial swelling and cytochrome c release in Atorvastatin treated mice, suggesting that mitochondrial ROS formation and apoptosis signaling are likely involved in Atorvastatin neurotoxicity. Importantly according to the data from animals receiving either CoQ10 or Vit E, supplementation with these lipophilic antioxidants, remarkable reverted the corresponding Atorvastatin mitochondrial toxicity markers. Concusion: Conclusively the present work addresses chronic Atorvastatin toxic effects on brain mitochondria and supports advantages of Vit E or CoQ10 supplementation. Whether such neurotoxic effect is correlated with CoQ10 depletion or if the improving effects of the due supplementation contribute toAtorvastatin receiving patients, needs more investigations.
{"title":"Toxicity of atorvastatin on isolated brain mitochondria","authors":"S. Nasoohi, P. Naserzadeh, Nafiseh Rahmanabadi, Banafsheh Saiid, Jalal Pourahmad Jaktaji","doi":"10.22034/ijps.2017.31107","DOIUrl":"https://doi.org/10.22034/ijps.2017.31107","url":null,"abstract":"Background: Although the bio kinetics, metabolism and chemical toxicity of Atorvastatin are well known, until recently little attention was paid to the potential neurotoxic effect of Atorvastatin (Atv). Regarding the concrete evidences indicating Atv may reduce Coenzyme Q10 (CoQ10) levels through blockage of metalonate cycle, the present work aims to determine if Atorvastatin may provide toxic effects on brain mitochondria and whether its supplementation with two lipidicantioxidants, CoQ10 and Vit E may improve such outcomes. Methods: to evaluate mitochondrial toxicity, male NMRI mice were first treated with Atorvastatin(bo; 20 and 60 mg/kg) every other day with or without supplementation with CoQ10 (200 mg/kg) or Vit E (40 u/kg). After a period of 4 weeks, the animals were euthanized and brain cortices were harvested ad subjected to mitochondria isolation procedure. ROS release, mitochondrial membrane potential (MMP) and cytochrome c release were performed to precisely address the probable mitochondrial injury. Findings: Our data showed increased ROS formation, MMP collapse, mitochondrial swelling and cytochrome c release in Atorvastatin treated mice, suggesting that mitochondrial ROS formation and apoptosis signaling are likely involved in Atorvastatin neurotoxicity. Importantly according to the data from animals receiving either CoQ10 or Vit E, supplementation with these lipophilic antioxidants, remarkable reverted the corresponding Atorvastatin mitochondrial toxicity markers. Concusion: Conclusively the present work addresses chronic Atorvastatin toxic effects on brain mitochondria and supports advantages of Vit E or CoQ10 supplementation. Whether such neurotoxic effect is correlated with CoQ10 depletion or if the improving effects of the due supplementation contribute toAtorvastatin receiving patients, needs more investigations.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"13 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45428950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-01DOI: 10.22034/IJPS.2017.31146
S. Emami, Rezvan Yazdian, A. Arab, M. Sadeghi, Z. Tayarani‐Najaran
Nepeta glomerulosa Boiss. is a medicinal plant has been used in traditional medicine. The aim of this study was to evaluate the anti-melanogenesis inhibitory activity of MeOH, n-hexane, ethyl Acetate (EtAc), dichloromethane (CH2Cl2), n-butanol (BuOH) and H2O extracts isolated from N. glomerulosa in B16 melanoma cell line. The B16F10 cell line viability after treatment with increasing concentrations of different extracts of the plant (50-200 µg/ml) was measured by using MTT, the inhibitory effect on synthesis of melanin, mushroom tyrosinase activity, cellular tyrosinase and effect on oxidative stress was determined by the colorimetric and fluorometric method. The data showed that at concentrations our results showed the melanogenesis inhibitory and antioxidant effects of N. glomerulosa on B16F10 cells may recommend a novel agent in diminishing skin pigmentation and skin aging in cosmetic industry.
{"title":"Anti-melanogenic activity of different extracts from aerial parts of Nepeta glomeruloasin on murine melanoma B16F10 cells","authors":"S. Emami, Rezvan Yazdian, A. Arab, M. Sadeghi, Z. Tayarani‐Najaran","doi":"10.22034/IJPS.2017.31146","DOIUrl":"https://doi.org/10.22034/IJPS.2017.31146","url":null,"abstract":"Nepeta glomerulosa Boiss. is a medicinal plant has been used in traditional medicine. The aim of this study was to evaluate the anti-melanogenesis inhibitory activity of MeOH, n-hexane, ethyl Acetate (EtAc), dichloromethane (CH2Cl2), n-butanol (BuOH) and H2O extracts isolated from N. glomerulosa in B16 melanoma cell line. The B16F10 cell line viability after treatment with increasing concentrations of different extracts of the plant (50-200 µg/ml) was measured by using MTT, the inhibitory effect on synthesis of melanin, mushroom tyrosinase activity, cellular tyrosinase and effect on oxidative stress was determined by the colorimetric and fluorometric method. The data showed that at concentrations our results showed the melanogenesis inhibitory and antioxidant effects of N. glomerulosa on B16F10 cells may recommend a novel agent in diminishing skin pigmentation and skin aging in cosmetic industry.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"13 1","pages":"61-74"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42433912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-01DOI: 10.22034/IJPS.2017.31144
N. Tanideh, M. Yavari, A. Seddighi, O. K. Hosseinabadi, M. Farshad, M. Mokhtari, B. Yeganeh, A. Jamshidzadeh, Sajad Daneshi, Behrouz Nikahval
In this study, we compared the effects of Calendula and Alpha ointment in the treatment of burn wounds and compare its results with silver sulfadiazine (SSD). Seventy-five male Sprague-Dawley rats were divided into five groups, and similar burn ulcers were produced on anterior surface of thigh of rats. The first group of rats no treatment was applied, base gel was applied topically to group II, in groups III-V, Alpha, SSD and Calendula preparations were applied, respectively. Wound healing, contraction and histopathological evaluation were evaluated at the end of 7, 14, and 21 days. Alpha ointment was equally effective as Calendula gel, and had better efficacy compared to SSD for all markers of wound healing at days of 7, 14 and 21. Alpha and Calendula preparations are less expensive drugs and significantly improve the quality of wound healing and scar formation and are more appropriate treatment choices than SSD. Therefore, we recommend them as alternative to SSD, especially in patients with low economical backgrounds or in those who show adverse reactions to SSD.
{"title":"Comparison between Alpha and Calendula for healing of third-degree burn in rats","authors":"N. Tanideh, M. Yavari, A. Seddighi, O. K. Hosseinabadi, M. Farshad, M. Mokhtari, B. Yeganeh, A. Jamshidzadeh, Sajad Daneshi, Behrouz Nikahval","doi":"10.22034/IJPS.2017.31144","DOIUrl":"https://doi.org/10.22034/IJPS.2017.31144","url":null,"abstract":"In this study, we compared the effects of Calendula and Alpha ointment in the treatment of burn wounds and compare its results with silver sulfadiazine (SSD). Seventy-five male Sprague-Dawley rats were divided into five groups, and similar burn ulcers were produced on anterior surface of thigh of rats. The first group of rats no treatment was applied, base gel was applied topically to group II, in groups III-V, Alpha, SSD and Calendula preparations were applied, respectively. Wound healing, contraction and histopathological evaluation were evaluated at the end of 7, 14, and 21 days. Alpha ointment was equally effective as Calendula gel, and had better efficacy compared to SSD for all markers of wound healing at days of 7, 14 and 21. Alpha and Calendula preparations are less expensive drugs and significantly improve the quality of wound healing and scar formation and are more appropriate treatment choices than SSD. Therefore, we recommend them as alternative to SSD, especially in patients with low economical backgrounds or in those who show adverse reactions to SSD.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"13 1","pages":"51-60"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44434978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-01DOI: 10.22034/ijps.2017.31154
M. Nakhjavani, F. Shirazi
In vitro studies on cancer cell lines still constitutes a major part of cancer research. It is very important that the researchers consider the importance of basic conditions of working with different cell lines. To the best of authors knowledge, this study is the first one to report the effect of seeding density on the growth curve of three human cancer cell lines. These cell lines include human breast adenocarcinoma MCF-7 cell line, human ovary carcinoma A2780 cell line and human melanoma A375 cell line. The cell lines were seeded at 4 different seeding densities: 4000, 5000, 15000 and 50000 cells/cm2 of culture surface. Following seeding the cells and on daily basis, cell count was done with trypan blue assay. The experiment was done in triplicate. The growth curves of the cells were constructed using Microsoft Excel and population doubling time (PDT), lag time (LT) and saturation density (SD) were calculated. The results showed that the seeding density affects the general pattern of the growth curve. At lower seeding densities, the growth curves have a more expected pattern rather than in higher seeding densities. Also, seeding density does not affect the LT, while it has some effects on SD and PDT.
{"title":"Reporting the Effect of Cell Seeding Density on Growth Pattern of Cancer Cell Lines","authors":"M. Nakhjavani, F. Shirazi","doi":"10.22034/ijps.2017.31154","DOIUrl":"https://doi.org/10.22034/ijps.2017.31154","url":null,"abstract":"In vitro studies on cancer cell lines still constitutes a major part of cancer research. It is very important that the researchers consider the importance of basic conditions of working with different cell lines. To the best of authors knowledge, this study is the first one to report the effect of seeding density on the growth curve of three human cancer cell lines. These cell lines include human breast adenocarcinoma MCF-7 cell line, human ovary carcinoma A2780 cell line and human melanoma A375 cell line. The cell lines were seeded at 4 different seeding densities: 4000, 5000, 15000 and 50000 cells/cm2 of culture surface. Following seeding the cells and on daily basis, cell count was done with trypan blue assay. The experiment was done in triplicate. The growth curves of the cells were constructed using Microsoft Excel and population doubling time (PDT), lag time (LT) and saturation density (SD) were calculated. The results showed that the seeding density affects the general pattern of the growth curve. At lower seeding densities, the growth curves have a more expected pattern rather than in higher seeding densities. Also, seeding density does not affect the LT, while it has some effects on SD and PDT.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"13 1","pages":"87-94"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41357420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-01DOI: 10.22034/IJPS.2017.31148
R. Badalzadeh, L. Chodari, V. Ghorbanzadeh
Type 1 diabetes is a chronic disease characterized by the body's inability to produce insulin due to destruction of the beta cells. There is increasing evidence that reactive oxygen species (ROS) play a major role in the development of diabetic complications. The purpose of this study is to investigate the effects of troxerutin administration on oxidative stress markers in blood of STZ-induced diabetic rats. Male Wistar rats were divided into 4 groups as: control (con), control-troxerutin (CON-TRX), diabetes (Dia), diabetic-troxerutin (DIA-TRX). Type 1 diabetes was induced by injection of streptozotocin (STZ) (i.p, 55mg/kg) and lasted for 10 weeks. Animals were received oral administration of troxerutin (150 mg/kg) for 4 weeks. At the end of study, malondialdehyde (MDA, the main product of lipid peroxidation), activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) were measured spectrophotometrically. Induction of diabetes with STZ resulted in increased MDA levels and decreased blood antioxidant capacity as compared with those of controls (P<0.05). Pre-treatment of diabetic rats with troxerutin significantly decreased the levels of MDA (P<0.01) and increased the activity of antioxidant enzymes SOD, GPX and CAT compared to untreated-diabetic groups. Troxerutin had no significant influence on non-diabetic rats. These findings showed that troxerutin may prevent oxidative complications of diabetic circumstances by elevating antioxidant enzymes activities and reducing lipid peroxidation.
{"title":"Troxerutin, a bioflavonoid, improves oxidative stress in blood of streptozotocin-induced type-1 diabetic rats","authors":"R. Badalzadeh, L. Chodari, V. Ghorbanzadeh","doi":"10.22034/IJPS.2017.31148","DOIUrl":"https://doi.org/10.22034/IJPS.2017.31148","url":null,"abstract":"Type 1 diabetes is a chronic disease characterized by the body's inability to produce insulin due to destruction of the beta cells. There is increasing evidence that reactive oxygen species (ROS) play a major role in the development of diabetic complications. The purpose of this study is to investigate the effects of troxerutin administration on oxidative stress markers in blood of STZ-induced diabetic rats. Male Wistar rats were divided into 4 groups as: control (con), control-troxerutin (CON-TRX), diabetes (Dia), diabetic-troxerutin (DIA-TRX). Type 1 diabetes was induced by injection of streptozotocin (STZ) (i.p, 55mg/kg) and lasted for 10 weeks. Animals were received oral administration of troxerutin (150 mg/kg) for 4 weeks. At the end of study, malondialdehyde (MDA, the main product of lipid peroxidation), activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) were measured spectrophotometrically. Induction of diabetes with STZ resulted in increased MDA levels and decreased blood antioxidant capacity as compared with those of controls (P<0.05). Pre-treatment of diabetic rats with troxerutin significantly decreased the levels of MDA (P<0.01) and increased the activity of antioxidant enzymes SOD, GPX and CAT compared to untreated-diabetic groups. Troxerutin had no significant influence on non-diabetic rats. These findings showed that troxerutin may prevent oxidative complications of diabetic circumstances by elevating antioxidant enzymes activities and reducing lipid peroxidation.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"13 1","pages":"75-86"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41516284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-01DOI: 10.22034/IJPS.2017.31130
J. Salamzadeh, M. Kamalinejad, B. Mofid, S. Mortazavi, Alireza Sheikhlar, M. Babaeian
Radiotherapy, a highly effective way to destroy breast cancer, causes skin adverse effects. A considerable amount of studies have been conducted to find a way to alleviate or relieve dermal adverse effects of radiation. The aim of this study was to observe the clinical effect of Elaeagnus angustifolia L. cream to treat radiotherapy-induced skin destruction in breast cancer patients. thirty two patients suffering from different stages of radiotherapy-induced skin reactions were evaluated in a double-blind randomized study; 16 patients in the treatment group received Elaeagnus angustifolia cream, while the other 16 patients, served as control group. Two weeks after the treatment, patients in Elaeagnus angustifolia group showed significantly lower skin reaction grade compared with placebo group. The attitude of the patients of Elaeagnus angustifolia group about dryness, itching, pain, burning, blisters and sores was significantly improved after two weeks of treatment. Elaeagnus angustifolia cream may effectively reduce the radiotherapy-induced dermal injury.
{"title":"The Effect of Elaeagnus angustifolia L. Cream on Radiation-Induced Skin Reactions in Women with Breast Cancer; A Preliminary Clinical Trial","authors":"J. Salamzadeh, M. Kamalinejad, B. Mofid, S. Mortazavi, Alireza Sheikhlar, M. Babaeian","doi":"10.22034/IJPS.2017.31130","DOIUrl":"https://doi.org/10.22034/IJPS.2017.31130","url":null,"abstract":"Radiotherapy, a highly effective way to destroy breast cancer, causes skin adverse effects. A considerable amount of studies have been conducted to find a way to alleviate or relieve dermal adverse effects of radiation. The aim of this study was to observe the clinical effect of Elaeagnus angustifolia L. cream to treat radiotherapy-induced skin destruction in breast cancer patients. thirty two patients suffering from different stages of radiotherapy-induced skin reactions were evaluated in a double-blind randomized study; 16 patients in the treatment group received Elaeagnus angustifolia cream, while the other 16 patients, served as control group. Two weeks after the treatment, patients in Elaeagnus angustifolia group showed significantly lower skin reaction grade compared with placebo group. The attitude of the patients of Elaeagnus angustifolia group about dryness, itching, pain, burning, blisters and sores was significantly improved after two weeks of treatment. Elaeagnus angustifolia cream may effectively reduce the radiotherapy-induced dermal injury.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"13 1","pages":"25-36"},"PeriodicalIF":0.0,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48274225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.22034/IJPS.2017.26642
seyed Abolfazli Abolfazli, M. Mohammadzadeh, F. Peiravian, Hesam Sharifnia
Cancer can be treated by surgery, chemotherapy, radiation therapy, hormonal therapy, and targeted therapy. The choice of treatment dependson the type and stage of the cancer. Chemotherapy drugs play an important role in cancer treatment. The objective of this interview-questionnaire study is to explore what influences Iranian hematologists-oncologists’ prescription when prescribing chemotherapy drug. Data collection can be divided into two categories: the interview and the questionnaire. The qualitative part consists of depthinterviews, utilizing closed- and open-ended questions, were conducted with oncologists, selected through theoretical sampling. The interview questions consisted of two parts, in the first part; open-ended questions were posed about factors influencing prescription. In the second part, the oncologists were asked to rate predefined factors that might influence their prescription decisions. The quantitative part of the study consists of questionnaire included two parts; the first part consisted of general questions including gender, age, membership in scientific committees and professional background. The second part consisted of 24 specialized questions that covered all the indexes of the effective factors on oncologists ‘prescription decisions.Ten oncologists were interviewed. Factors affecting chemotherapy drug' prescription were grouped into three categories based on qualitative studies carried out and is presented as they were mentioned and rated by the hematologists-oncologists: 1) Products properties, 2) Pharmaceutical marketing, 3) Scientific, experimental confirmation. The findings from the questionnaire suggest products properties particularly (clinical effectiveness) is the most important factor considered in drug prescription choice.Also, the socialized medicine sponsorship and clinical trial respectively are the most effective on increasing the frequency of prescribing. Whilst, prescribing by brand name or higher prices for adrug are not necessarily justified. The results of this study provide new insights to the nature prescribing behavior driven to develop measures which could be used to achieve greater clinical effectiveness and economic efficiency from drug prescribing.
{"title":"Factors influencing hematologists-oncologists’ prescription of chemotherapy drugs in cancer treatment in Iran: aninterview-questionnaire study","authors":"seyed Abolfazli Abolfazli, M. Mohammadzadeh, F. Peiravian, Hesam Sharifnia","doi":"10.22034/IJPS.2017.26642","DOIUrl":"https://doi.org/10.22034/IJPS.2017.26642","url":null,"abstract":"Cancer can be treated by surgery, chemotherapy, radiation therapy, hormonal therapy, and targeted therapy. The choice of treatment dependson the type and stage of the cancer. Chemotherapy drugs play an important role in cancer treatment. The objective of this interview-questionnaire study is to explore what influences Iranian hematologists-oncologists’ prescription when prescribing chemotherapy drug. Data collection can be divided into two categories: the interview and the questionnaire. The qualitative part consists of depthinterviews, utilizing closed- and open-ended questions, were conducted with oncologists, selected through theoretical sampling. The interview questions consisted of two parts, in the first part; open-ended questions were posed about factors influencing prescription. In the second part, the oncologists were asked to rate predefined factors that might influence their prescription decisions. The quantitative part of the study consists of questionnaire included two parts; the first part consisted of general questions including gender, age, membership in scientific committees and professional background. The second part consisted of 24 specialized questions that covered all the indexes of the effective factors on oncologists ‘prescription decisions.Ten oncologists were interviewed. Factors affecting chemotherapy drug' prescription were grouped into three categories based on qualitative studies carried out and is presented as they were mentioned and rated by the hematologists-oncologists: 1) Products properties, 2) Pharmaceutical marketing, 3) Scientific, experimental confirmation. The findings from the questionnaire suggest products properties particularly (clinical effectiveness) is the most important factor considered in drug prescription choice.Also, the socialized medicine sponsorship and clinical trial respectively are the most effective on increasing the frequency of prescribing. Whilst, prescribing by brand name or higher prices for adrug are not necessarily justified. The results of this study provide new insights to the nature prescribing behavior driven to develop measures which could be used to achieve greater clinical effectiveness and economic efficiency from drug prescribing.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"13 1","pages":"7-22"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68023267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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