{"title":"The Effect of Streptozotocin-induced Diabetes on Bone Tissue around Endosseous Implants after Osseointegration","authors":"Toshitake Obata, A. Yokoyama, Satoru Yamamoto, T. Kawasaki, Mitsutaka Oda, T. Iizuka, T. Kohgo","doi":"10.2330/JORALBIOSCI1965.44.19","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.19","url":null,"abstract":"本研究では, 糖尿病がosseointegration獲得後のチタンインプラント周囲の骨組織に与える影響について, streptozotocin誘発糖尿病ラットを用いて組織学的, 組織計量学的に検討を加えた. ラット大腿骨にチタンンプラントを埋入し, 8週後にstreptozotocinを腹腔内に投与することにより糖尿病を誘発させ, 4週後に安楽死させた. 対照はチタンインプラント埋入後8週および12週のラットとした. 骨-インプラント体の接触率は, 糖尿病ラットと対照群のラットの間に有意差は認められず, 糖尿病ラットではインプラント体周囲の新生骨量が有意に低い値を示した. 以上の結果から, 4週間の高血糖状態は, 獲得されたosseointegrationを破壊することはなく, インプラント周囲の骨形成を抑制することが示された.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"28 1","pages":"19-28"},"PeriodicalIF":0.0,"publicationDate":"2002-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75609066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-02-20DOI: 10.2330/JORALBIOSCI1965.44.48
T. Hiratsuka, Kaori Sato, T. Aoba
Much attention has been given to the growth mechanism of enamel mineral by which the unique features (i. e., morphology, size and alignment) of enamel crystallites in mammals are achieved. In this paper, we report our experimental and theoretical approaches to a long-debated and unsettled issue, i. e., the involvement and role of an acidic precursor in enamel mineralization. Analysis of nucleation events, which were induced in neutral media at 37°C and 1.8% partial CO2 pressure, substantiated the involvement of octacalcium phosphate (OCP) in precipitation reactions. We also developed a new crystal growth model using miniaturized columns in tandem, in which OCP itself was proved to be equally competent, in comparison with hydroxyapatite (HA), as a template for crystal precipitation and as an adsorbent for interaction with enamel proteins. Simulation of the precipitation process taking place in situ with calcium transport (its increment) into enamel fluid also allowed us to predict that OCP is more kinetically favorable than HA, if Ca ions are supplied through the cellular activity of ameloblasts. The results obtained support the theory that OCP is involved as an inevitable intermediate phase to yield large enamel carbonatoapatite during amelogenesis.
{"title":"Thermodynamic and Kinetic Interpretation of Enamel Mineralization Octacalcium Phosphate (OCP) as an Inevitable Intermediate Phase","authors":"T. Hiratsuka, Kaori Sato, T. Aoba","doi":"10.2330/JORALBIOSCI1965.44.48","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.48","url":null,"abstract":"Much attention has been given to the growth mechanism of enamel mineral by which the unique features (i. e., morphology, size and alignment) of enamel crystallites in mammals are achieved. In this paper, we report our experimental and theoretical approaches to a long-debated and unsettled issue, i. e., the involvement and role of an acidic precursor in enamel mineralization. Analysis of nucleation events, which were induced in neutral media at 37°C and 1.8% partial CO2 pressure, substantiated the involvement of octacalcium phosphate (OCP) in precipitation reactions. We also developed a new crystal growth model using miniaturized columns in tandem, in which OCP itself was proved to be equally competent, in comparison with hydroxyapatite (HA), as a template for crystal precipitation and as an adsorbent for interaction with enamel proteins. Simulation of the precipitation process taking place in situ with calcium transport (its increment) into enamel fluid also allowed us to predict that OCP is more kinetically favorable than HA, if Ca ions are supplied through the cellular activity of ameloblasts. The results obtained support the theory that OCP is involved as an inevitable intermediate phase to yield large enamel carbonatoapatite during amelogenesis.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"29 1","pages":"48-65"},"PeriodicalIF":0.0,"publicationDate":"2002-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82291207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-02-20DOI: 10.2330/JORALBIOSCI1965.44.66
Y. Seki, N. Ishii, K. Toda, K. Soma
It has been critical in clinical dentistry to understand the response properties of periodontal mechanoreceptors (PMRs) when the occlusal condition was changed, because the PMRs are important sensory receptors controlling jaw movement. Therefore, we investigated the effect of opposed tooth loss on the response properties of PMRs in rat molars. All mandibular molars in an in vitro jaw-nerve preparation, made from a rat without opposed teeth, were carefully extracted before electrophysiological recordings. Single-unit discharges evoked by direct mechanical stimuli with von Frey hairs applied to the PMRs were recorded from the inferior alveolar nerve on day 3, and 1, 2, 4, 8, and 12 weeks after opposed tooth extraction. The following results were obtained; (1) in both the 3-day and 1-week post-extraction groups, the von Frey threshold value was significantly lower, although in the 12-week group, it was significantly higher than that in the control group and (2) in the 3-day to 8-week groups, the conduction velocity of nerve fibers innervating PMRs was significantly slower than that in the control group. These results showed that sustained functional changes could be clearly elicited in PMRs by occlusal hypofunction, suggesting that these changes may have critical influences on both jaw reflexes and masticatory movements.
{"title":"Influence of Occlusal Hypofunction Induced by Opposed Tooth Loss on Periodontal Mechanoreceptors in Rat Molars","authors":"Y. Seki, N. Ishii, K. Toda, K. Soma","doi":"10.2330/JORALBIOSCI1965.44.66","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.66","url":null,"abstract":"It has been critical in clinical dentistry to understand the response properties of periodontal mechanoreceptors (PMRs) when the occlusal condition was changed, because the PMRs are important sensory receptors controlling jaw movement. Therefore, we investigated the effect of opposed tooth loss on the response properties of PMRs in rat molars. All mandibular molars in an in vitro jaw-nerve preparation, made from a rat without opposed teeth, were carefully extracted before electrophysiological recordings. Single-unit discharges evoked by direct mechanical stimuli with von Frey hairs applied to the PMRs were recorded from the inferior alveolar nerve on day 3, and 1, 2, 4, 8, and 12 weeks after opposed tooth extraction. The following results were obtained; (1) in both the 3-day and 1-week post-extraction groups, the von Frey threshold value was significantly lower, although in the 12-week group, it was significantly higher than that in the control group and (2) in the 3-day to 8-week groups, the conduction velocity of nerve fibers innervating PMRs was significantly slower than that in the control group. These results showed that sustained functional changes could be clearly elicited in PMRs by occlusal hypofunction, suggesting that these changes may have critical influences on both jaw reflexes and masticatory movements.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"1 1","pages":"66-74"},"PeriodicalIF":0.0,"publicationDate":"2002-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89668746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-02-20DOI: 10.2330/JORALBIOSCI1965.44.1
Y. Asada, Yukie Sato, Takehiko Shimizu, T. Sakae, Hirotsugu Yamamoto, T. Maeda
The aim of this study was to elucidate the three genotypes (op/op, op/+ and +/+) of heterogenous osteopetrotic mice using microsatellite markers. The genotyping analysis was performed on genetic crosses using B6C3Fe-op/+ and A/WySnJ mice. In this study, we used five MIT markers, D3Mit11, 75, 101, 102 and 189 that are closely linked to the op locus and which segregate polymorphic variants in mating between B6C3Fe-op/+ and A/WySnJ mice, and the homozygous op/op F3 mice were obtained by F2 mice mating. From the results of F3 genotyping, it was possible to determine the genotype of the op gene without a complicated molecular process. Histopathological studies revealed that the F3-op/op mice showed similar femur bone structures to those of B6C3Fe-op/op mice. Based on these findings, it is suggested that genotyping of heterogenous osteopetrotic mice using microsatellite markers is a powerful technique for investigating the mechanism of osteoclast development and genetic studies of osteopetrosis. 抄録:大 理石 骨病 交雑系 モ デルマ ウス を用 い,マ イク ロサ テ ライ トマー カー によ る遺伝子 型 を検 出 す るこ とで op遺 伝子 の3つ の遺 伝子型(op/op, op/+, +/+)を 判定 で きるか否 か を 目的 と し本実験 を行 った。B6C3Feop/+マ ウス とA/WySnJマ ウ ス との交配 よ り得 られ た すべ て のF2マ ウス の遺 伝 子型 をマ イ ク ロサ テ ライ ト マー カー にて判 定 した と ころ,す べて のマ ウスで表現型(切 歯 の萌 出の有無)と 一 致 していた。 さらに,肉 眼 的 に は+/+と 判別 困難 なヘ テ ロ型(op/+)マ ウス をマイ クロサ テライ トマー カー よ り判定 し,選 抜 したF2ヘ テ ロ 型 マ ウス雌雄 を交 配 した ところ,F3マ ウス においてop/opマ ウスを作 成す るこ とがで きた。また,組 織 学的所 見 よ り従 来 のopマ ウス とF3-op/opマ ウスで は大 腿骨 にお ける形 態学 的特徴 が類似 して いた。以 上 の結果 よ り,大 理 石骨病 交雑 系 モデル マ ウスはマ イ クロサテ ライ トマーカー を用 いる こ とで簡 便 に遺 伝子 型 を判 定で きる ことが 明 らか とな り,破 骨 細胞 の分化 に対 す るメ カニ ズム解明 な らび に大理 石骨病 の遺伝 学的研 究 に有 用で あ る ことが
{"title":"Genotyping of Heterogenous Osteopetrotic Mice Using Microsatellite Markers","authors":"Y. Asada, Yukie Sato, Takehiko Shimizu, T. Sakae, Hirotsugu Yamamoto, T. Maeda","doi":"10.2330/JORALBIOSCI1965.44.1","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.1","url":null,"abstract":"The aim of this study was to elucidate the three genotypes (op/op, op/+ and +/+) of heterogenous osteopetrotic mice using microsatellite markers. The genotyping analysis was performed on genetic crosses using B6C3Fe-op/+ and A/WySnJ mice. In this study, we used five MIT markers, D3Mit11, 75, 101, 102 and 189 that are closely linked to the op locus and which segregate polymorphic variants in mating between B6C3Fe-op/+ and A/WySnJ mice, and the homozygous op/op F3 mice were obtained by F2 mice mating. From the results of F3 genotyping, it was possible to determine the genotype of the op gene without a complicated molecular process. Histopathological studies revealed that the F3-op/op mice showed similar femur bone structures to those of B6C3Fe-op/op mice. Based on these findings, it is suggested that genotyping of heterogenous osteopetrotic mice using microsatellite markers is a powerful technique for investigating the mechanism of osteoclast development and genetic studies of osteopetrosis. 抄録:大 理石 骨病 交雑系 モ デルマ ウス を用 い,マ イク ロサ テ ライ トマー カー によ る遺伝子 型 を検 出 す るこ とで op遺 伝子 の3つ の遺 伝子型(op/op, op/+, +/+)を 判定 で きるか否 か を 目的 と し本実験 を行 った。B6C3Feop/+マ ウス とA/WySnJマ ウ ス との交配 よ り得 られ た すべ て のF2マ ウス の遺 伝 子型 をマ イ ク ロサ テ ライ ト マー カー にて判 定 した と ころ,す べて のマ ウスで表現型(切 歯 の萌 出の有無)と 一 致 していた。 さらに,肉 眼 的 に は+/+と 判別 困難 なヘ テ ロ型(op/+)マ ウス をマイ クロサ テライ トマー カー よ り判定 し,選 抜 したF2ヘ テ ロ 型 マ ウス雌雄 を交 配 した ところ,F3マ ウス においてop/opマ ウスを作 成す るこ とがで きた。また,組 織 学的所 見 よ り従 来 のopマ ウス とF3-op/opマ ウスで は大 腿骨 にお ける形 態学 的特徴 が類似 して いた。以 上 の結果 よ り,大 理 石骨病 交雑 系 モデル マ ウスはマ イ クロサテ ライ トマーカー を用 いる こ とで簡 便 に遺 伝子 型 を判 定で きる ことが 明 らか とな り,破 骨 細胞 の分化 に対 す るメ カニ ズム解明 な らび に大理 石骨病 の遺伝 学的研 究 に有 用で あ る ことが","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"22 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2002-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91545338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-02-20DOI: 10.2330/JORALBIOSCI1965.44.29
Y. Kitano, T. Hara, Y. Ide
The purpose of this study was to clarify the distribution of the inferior alveolar artery (IAA) and its dental branches in the mandibular canal and surrounding tissues in both dentulous and edent ulous mandibles using a Micro-CT system. Twenty-six sides of 18 mandibles from Japanese cadavers were examined . Contrast medium was injected into the maxillary artery of the examined specimens to obtain the Micro-CT and 3-D reconstruction images . Maximum vertical length and horizontal breadth of the mandibular canal were measured to determi ne the position of IAA in the canal. The angle of the dental branches against IAA and the inside diameter of IAA were also examined. The vertical position of IAA in the molar area was higher in the edentulous than that in the dent ulous mandible. There was, however, no significant difference in the horizontal position . The retromolar ramus , i n which the dental branches were distributed at the retromolar region , was usually observed to run toward the buccal just after separating from IAA. The inside diameter of IAA was smaller in the edentulous tha n that in the dentulous mandible. These results suggest that positional change of IAA in the mandibular canal and decrease of inside diameter may be caused by absorption of alveolar bone following tooth loss and subsequently morphological change of the mandibular canal. However, the running direction of the retromolar ramus did not change . 抄録:有 歯顎と無歯顎における下歯槽動脈の走行形態および歯枝の分岐形態を把握することを目的として, Micro-CTを 用いた三次元的観察および解析を行った。試料として顎動脈より造影剤の注入を行った日本人成人 遺体18体26側 を用いた。下顎管を基準 とした下歯槽動脈の垂直的位置,水 平的な位置,血 管内径および下歯槽 動脈を基準 とした歯枝の分岐する角度の計測を行ったところ,以 下の結果を得た。下顎管内部における下歯槽動 〒261-8502 千 葉 県千葉 市美浜 区真 砂1-2-2 30 歯 基 礎 誌44: 29-39, 2002. 脈は,無 歯顎になると垂直的には有歯顎と比較して相対的に上方に位置したが,水 平的には差が認められなかっ た。血管内径は無歯顎になると細くなる傾向が認められた。歯枝は歯牙の喪失により分岐する数の減少がみられ たが,臼 後領域に向かう臼後枝は変化が認められなかった。また,そ の臼後枝は頬側方向に分岐していた。これ らは,歯 牙喪失による歯槽骨の吸収,そ れに伴う内部構造,特 に下顎管の構造の変化が大きく関与していた。
{"title":"Three-dimensional Observation of the Distribution of the Inferior Alveolar Artery Using Micro-CT","authors":"Y. Kitano, T. Hara, Y. Ide","doi":"10.2330/JORALBIOSCI1965.44.29","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.29","url":null,"abstract":"The purpose of this study was to clarify the distribution of the inferior alveolar artery (IAA) and its dental branches in the mandibular canal and surrounding tissues in both dentulous and edent ulous mandibles using a Micro-CT system. Twenty-six sides of 18 mandibles from Japanese cadavers were examined . Contrast medium was injected into the maxillary artery of the examined specimens to obtain the Micro-CT and 3-D reconstruction images . Maximum vertical length and horizontal breadth of the mandibular canal were measured to determi ne the position of IAA in the canal. The angle of the dental branches against IAA and the inside diameter of IAA were also examined. The vertical position of IAA in the molar area was higher in the edentulous than that in the dent ulous mandible. There was, however, no significant difference in the horizontal position . The retromolar ramus , i n which the dental branches were distributed at the retromolar region , was usually observed to run toward the buccal just after separating from IAA. The inside diameter of IAA was smaller in the edentulous tha n that in the dentulous mandible. These results suggest that positional change of IAA in the mandibular canal and decrease of inside diameter may be caused by absorption of alveolar bone following tooth loss and subsequently morphological change of the mandibular canal. However, the running direction of the retromolar ramus did not change . 抄録:有 歯顎と無歯顎における下歯槽動脈の走行形態および歯枝の分岐形態を把握することを目的として, Micro-CTを 用いた三次元的観察および解析を行った。試料として顎動脈より造影剤の注入を行った日本人成人 遺体18体26側 を用いた。下顎管を基準 とした下歯槽動脈の垂直的位置,水 平的な位置,血 管内径および下歯槽 動脈を基準 とした歯枝の分岐する角度の計測を行ったところ,以 下の結果を得た。下顎管内部における下歯槽動 〒261-8502 千 葉 県千葉 市美浜 区真 砂1-2-2 30 歯 基 礎 誌44: 29-39, 2002. 脈は,無 歯顎になると垂直的には有歯顎と比較して相対的に上方に位置したが,水 平的には差が認められなかっ た。血管内径は無歯顎になると細くなる傾向が認められた。歯枝は歯牙の喪失により分岐する数の減少がみられ たが,臼 後領域に向かう臼後枝は変化が認められなかった。また,そ の臼後枝は頬側方向に分岐していた。これ らは,歯 牙喪失による歯槽骨の吸収,そ れに伴う内部構造,特 に下顎管の構造の変化が大きく関与していた。","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"1 1","pages":"29-39"},"PeriodicalIF":0.0,"publicationDate":"2002-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87465213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-12-20DOI: 10.2330/JORALBIOSCI1965.43.659
Tomokazu Nakajima, R. Fujisawa, Y. Kuboki
Phosphophoryn (PP) is a unique phosphoprotein of dentin . Previous investigators have reported that PP degrades during tooth maturation, but its degradation mechanism has not been determined . In this study, we attempted to examine the susceptibility of PP to proteases , using SDS-PAGE with sensitive staining, and we discussed the mechanisms of PP degradation . A human molar, divided into crown and root, was analyzed by SDS-PAGE. The crown fragment contained more lower molecular weight components than the root fragment, indicating that human PP is degraded in vivo during maturation . To examine the mechanisms of the degradation, we investigated the susceptibility of PP to proteases and heat treatment . Rat PP preparations were subjected to matrix metalloproteinase-2 (MMP-2) , trypsin or heat treatment. Major bands of rat PP were observed at 74 and 70kDa. These two bands were clearly observed in the sample treated with MMP-2 or trypsin, but in the heat-treated sample, the proteins corresponding to the major bands were degraded and only diffuse staining was observed . Enzymatic PP degradation was not observed for MMP-2 and trypsin. The non-enzymatic degradation, such as β elimination , as well as enzymatic processes by other MMPs, might contribute to PP degradation. 抄録:象 牙 質 ホス ホホ リン(PP)は,象 牙質 特有 の タンパ ク質 で あ る。 これ までの研 究 に よ り,歯 の成熟 に伴 いPPが 分 解 を受 け る ことが報 告 され てい るが,そ の分 解機構 は明 らか で はない。本研 究 で は,PPの プ ロテ アー ゼ に対 す る感受 性 をゲル 電気泳 動で 高感度 染色法 を用 い て検 討 し,PPの 分 解機構 につ いて考察 した 。ヒ ト第3大 臼歯 を歯冠 と歯 根 に分割 し,ゲ ル電気 泳動 で分析 した ところ,歯 冠 の ほ うが歯 根 よ り分 子量 分布 が,低 分子 量側 ヘ シフ トして いた。 これ は,ヒ トPPが,in vivoで 分 解 を受 ける こ とを示 して い る 。分 解機構 の解 明 のた め,PP の プロテ アーゼ(MMP-2,ト リプシ ン)ま た は,熱 処理 に対 す る感 受性 を検討 した。 ラ ッ トPPの 主 なバ ン ドは, 74と70kDaに み られ た。MMP-2,ト リプ シン を作 用 させ たサ ンプル は,主 要 なバ ン ドに変化 はな くPPの 分解 はみ られな か った。 熱処 理 した サ ンプルで は主要 なバ ン ドは形 成 されず,diffuseな 染色性 を示 し,PPの 分 解が み られ た。本 研究 で は,MMP-2,ト リプシ ンに関 して,酵 素的分 解 はみ られな かっ た。PPの 分 解 はほか のMMPs によ る酵 素的分 解 と同様 に,非 酵 素的分 解,β 脱 離反 応が重 要 であ る と思わ れた。
{"title":"Evaluation of the Susceptibility of Dentin Phosphoproteins to Proteases and Heat Treatment.","authors":"Tomokazu Nakajima, R. Fujisawa, Y. Kuboki","doi":"10.2330/JORALBIOSCI1965.43.659","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.659","url":null,"abstract":"Phosphophoryn (PP) is a unique phosphoprotein of dentin . Previous investigators have reported that PP degrades during tooth maturation, but its degradation mechanism has not been determined . In this study, we attempted to examine the susceptibility of PP to proteases , using SDS-PAGE with sensitive staining, and we discussed the mechanisms of PP degradation . A human molar, divided into crown and root, was analyzed by SDS-PAGE. The crown fragment contained more lower molecular weight components than the root fragment, indicating that human PP is degraded in vivo during maturation . To examine the mechanisms of the degradation, we investigated the susceptibility of PP to proteases and heat treatment . Rat PP preparations were subjected to matrix metalloproteinase-2 (MMP-2) , trypsin or heat treatment. Major bands of rat PP were observed at 74 and 70kDa. These two bands were clearly observed in the sample treated with MMP-2 or trypsin, but in the heat-treated sample, the proteins corresponding to the major bands were degraded and only diffuse staining was observed . Enzymatic PP degradation was not observed for MMP-2 and trypsin. The non-enzymatic degradation, such as β elimination , as well as enzymatic processes by other MMPs, might contribute to PP degradation. 抄録:象 牙 質 ホス ホホ リン(PP)は,象 牙質 特有 の タンパ ク質 で あ る。 これ までの研 究 に よ り,歯 の成熟 に伴 いPPが 分 解 を受 け る ことが報 告 され てい るが,そ の分 解機構 は明 らか で はない。本研 究 で は,PPの プ ロテ アー ゼ に対 す る感受 性 をゲル 電気泳 動で 高感度 染色法 を用 い て検 討 し,PPの 分 解機構 につ いて考察 した 。ヒ ト第3大 臼歯 を歯冠 と歯 根 に分割 し,ゲ ル電気 泳動 で分析 した ところ,歯 冠 の ほ うが歯 根 よ り分 子量 分布 が,低 分子 量側 ヘ シフ トして いた。 これ は,ヒ トPPが,in vivoで 分 解 を受 ける こ とを示 して い る 。分 解機構 の解 明 のた め,PP の プロテ アーゼ(MMP-2,ト リプシ ン)ま た は,熱 処理 に対 す る感 受性 を検討 した。 ラ ッ トPPの 主 なバ ン ドは, 74と70kDaに み られ た。MMP-2,ト リプ シン を作 用 させ たサ ンプル は,主 要 なバ ン ドに変化 はな くPPの 分解 はみ られな か った。 熱処 理 した サ ンプルで は主要 なバ ン ドは形 成 されず,diffuseな 染色性 を示 し,PPの 分 解が み られ た。本 研究 で は,MMP-2,ト リプシ ンに関 して,酵 素的分 解 はみ られな かっ た。PPの 分 解 はほか のMMPs によ る酵 素的分 解 と同様 に,非 酵 素的分 解,β 脱 離反 応が重 要 であ る と思わ れた。","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"85 1","pages":"659-665"},"PeriodicalIF":0.0,"publicationDate":"2001-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77607365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-12-20DOI: 10.2330/JORALBIOSCI1965.43.690
Kazuyuki Saito, Y. Asada
The purpose of this study was to identify the major candidate chromosome and to detect the region that included the candidate gene (s) causing absence of the third molars in EL/sea mice. The candidate chromosomal analysis was performed on genetic crosses using two strains of mice, EL with absence of the third molars, and DDY with a normal complement of teeth. Linkage analysis by interval mapping with microsatellite markers suggested that mouse chromosome 13 was one of the candidate chromosomes and therefore this chromosome was investigated in detail by individual genotyping of F 2 intercross mice with missing third molars. The highest scores were found at D13Mit78 and D13Mit35 markers (xx2=11.8, p< 0.01). Based on these findings, it is suggested that the gene causing absent third molars in EL mice maps close to these microsatellite loci.
{"title":"Mapping of a Gene Causing Mouse Hypodontia to Chromosome 13","authors":"Kazuyuki Saito, Y. Asada","doi":"10.2330/JORALBIOSCI1965.43.690","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.690","url":null,"abstract":"The purpose of this study was to identify the major candidate chromosome and to detect the region that included the candidate gene (s) causing absence of the third molars in EL/sea mice. The candidate chromosomal analysis was performed on genetic crosses using two strains of mice, EL with absence of the third molars, and DDY with a normal complement of teeth. Linkage analysis by interval mapping with microsatellite markers suggested that mouse chromosome 13 was one of the candidate chromosomes and therefore this chromosome was investigated in detail by individual genotyping of F 2 intercross mice with missing third molars. The highest scores were found at D13Mit78 and D13Mit35 markers (xx2=11.8, p< 0.01). Based on these findings, it is suggested that the gene causing absent third molars in EL mice maps close to these microsatellite loci.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"4 1","pages":"690-699"},"PeriodicalIF":0.0,"publicationDate":"2001-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83247286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-12-20DOI: 10.2330/JORALBIOSCI1965.43.666
H. Inokuchi, M. Mizuno-Kamiya, A. Fujita
{"title":"Comparison of Ca2+-Independent Phospholipase A2 Activity in Apical Plasma Membranes and Secretory Granules from the Rat Parotid Gland","authors":"H. Inokuchi, M. Mizuno-Kamiya, A. Fujita","doi":"10.2330/JORALBIOSCI1965.43.666","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.666","url":null,"abstract":"","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"10 1","pages":"666-675"},"PeriodicalIF":0.0,"publicationDate":"2001-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73121930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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