Pub Date : 2003-02-20DOI: 10.2330/JORALBIOSCI1965.45.8
Tadaoki Fukuizumi, T. Ohkubo, K. Kitamura
Streptozotocin-induced diabetic mice displayed a significantly lower mechanical nociceptive threshold than age-matched control mice. In centrast, there was no difference between diabetic mice and control mice in thermal nociceptive threshold. Intrathecal (i. t.) administration of ω-agatoxin IVA (0.33-10 pmol/mouse), a selective blocker of P/Q-type voltage-dependent calcium channels (VDCCs), produced dosedependent inhibition of the mechanical nociceptive response, and its antinociceptive effect at lower doses was greater in diabetic mice than in control mice. The antinociceptive effects of an N-type blocker ω-conotoxin GVIA (0.33-10pmol/mouse, i. t.) and an L-type blocker calciseptine (1-10pmol/mouse, i. t.) were both slightly, but not significantly greater in mice receiving streptozotocin. The antinociception of intrathecal morphine was not different between the experimental groups. Our results indicate that a selective alteration in the role of P/Q-type channels may occur in the spinal processing of mechanic l hypersensitivity in diabetic mice.
{"title":"Involvement of P/Q-type Voltage-dependent Calcium Channels in the Streptozotocin-induced Hyperalgesia in Mice","authors":"Tadaoki Fukuizumi, T. Ohkubo, K. Kitamura","doi":"10.2330/JORALBIOSCI1965.45.8","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.45.8","url":null,"abstract":"Streptozotocin-induced diabetic mice displayed a significantly lower mechanical nociceptive threshold than age-matched control mice. In centrast, there was no difference between diabetic mice and control mice in thermal nociceptive threshold. Intrathecal (i. t.) administration of ω-agatoxin IVA (0.33-10 pmol/mouse), a selective blocker of P/Q-type voltage-dependent calcium channels (VDCCs), produced dosedependent inhibition of the mechanical nociceptive response, and its antinociceptive effect at lower doses was greater in diabetic mice than in control mice. The antinociceptive effects of an N-type blocker ω-conotoxin GVIA (0.33-10pmol/mouse, i. t.) and an L-type blocker calciseptine (1-10pmol/mouse, i. t.) were both slightly, but not significantly greater in mice receiving streptozotocin. The antinociception of intrathecal morphine was not different between the experimental groups. Our results indicate that a selective alteration in the role of P/Q-type channels may occur in the spinal processing of mechanic l hypersensitivity in diabetic mice.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"102 1 1","pages":"8-15"},"PeriodicalIF":0.0,"publicationDate":"2003-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90992267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-12-20DOI: 10.2330/JORALBIOSCI1965.44.541
S. Ohba, T. T. Baba, T. Nemoto, T. Inokuchi
Scar formation, caused by the abnormal expression and accumulation of collagen molecules accompanying the expression of heat shock protein 47 (Hsp47), a collagen-specific molecular chaperone, is a serious problem after surgery in the postnate, whereas in the fetus, the wound heals without scarring. In this study, we compared the expression patterns of Hsp47 and type I and type III collagens induced by TGF-β1 between fetal and neonatal fibroblasts in the primary cultures of rat tongues. In the neonate, high level expressions of both Hsp47 and collagen mRNAs and proteins were observed to be induced by TGF-β1, but not in the fetus. We performed a reporter assay using pLUC 5.5 (III), which carried the enhancer/promoter region of the mouse Hsp47 gene, to compare promoter/reporter activity. In the neonate, high promoter/ reporter activity in fibroblasts treated with TGF-β1 was observed, but was unchanged in the fetus. Thus, the expression of Hsp47 is enhanced by TGF-β1 in the neonate but not in the fetus. This different Hsp47 expression pattern between the fetus and neonate appears to be attributed to the different transcriptional regulation of the gene. Elucidation of the regulatory mechanism of Hsp47 production in developmental processes may provide a therapeutic modality for the scarless healing of postnatal wounds.
{"title":"Expression of Hsp47 in Fibroblasts Derived from Fetal and Neonatal Rat Tongues","authors":"S. Ohba, T. T. Baba, T. Nemoto, T. Inokuchi","doi":"10.2330/JORALBIOSCI1965.44.541","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.541","url":null,"abstract":"Scar formation, caused by the abnormal expression and accumulation of collagen molecules accompanying the expression of heat shock protein 47 (Hsp47), a collagen-specific molecular chaperone, is a serious problem after surgery in the postnate, whereas in the fetus, the wound heals without scarring. In this study, we compared the expression patterns of Hsp47 and type I and type III collagens induced by TGF-β1 between fetal and neonatal fibroblasts in the primary cultures of rat tongues. In the neonate, high level expressions of both Hsp47 and collagen mRNAs and proteins were observed to be induced by TGF-β1, but not in the fetus. We performed a reporter assay using pLUC 5.5 (III), which carried the enhancer/promoter region of the mouse Hsp47 gene, to compare promoter/reporter activity. In the neonate, high promoter/ reporter activity in fibroblasts treated with TGF-β1 was observed, but was unchanged in the fetus. Thus, the expression of Hsp47 is enhanced by TGF-β1 in the neonate but not in the fetus. This different Hsp47 expression pattern between the fetus and neonate appears to be attributed to the different transcriptional regulation of the gene. Elucidation of the regulatory mechanism of Hsp47 production in developmental processes may provide a therapeutic modality for the scarless healing of postnatal wounds.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"26 1","pages":"541-548"},"PeriodicalIF":0.0,"publicationDate":"2002-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82277710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-12-20DOI: 10.2330/JORALBIOSCI1965.44.522
Kiyoshi Daito, Y. Azuma, M. Daito, K. Ohura
Prostaglandin D2 (PGD2) acts via the adenyl cyclase-coupled receptor for PGD2 (DP receptor). Here we present evidence that BW245C, a DP receptor agonist, modulates macrophage functions related to natunal immunity. BW245C inhibited macrophage chemotaxis at concentrations of 0.1 to 10μM and phagocytosis of Escherichia coli by macrophages at a concentration of 10μM. In addition, BW245C inhibited the production of superoxide anions by PMA-stimulated macrophages at concentrations of 0.1 to 10μM and nitrite production by LPS-stimulated macrophages at a concentration of 10μM. In contrast, BW245C potentiated the production of TNF-α, a pro-inflammatory cytokine, by LPS-stimulated macrophages at concentrations of 1 to 10μM. These results suggest that PGD2 may modulate macrophage functions related to natural immunity via the DP receptor.
{"title":"Simultaneous Determination of Alteration of a Variety of Macrophage Functions Related to Natural Immunity Following Treatment with a DP Receptor Agonist","authors":"Kiyoshi Daito, Y. Azuma, M. Daito, K. Ohura","doi":"10.2330/JORALBIOSCI1965.44.522","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.522","url":null,"abstract":"Prostaglandin D2 (PGD2) acts via the adenyl cyclase-coupled receptor for PGD2 (DP receptor). Here we present evidence that BW245C, a DP receptor agonist, modulates macrophage functions related to natunal immunity. BW245C inhibited macrophage chemotaxis at concentrations of 0.1 to 10μM and phagocytosis of Escherichia coli by macrophages at a concentration of 10μM. In addition, BW245C inhibited the production of superoxide anions by PMA-stimulated macrophages at concentrations of 0.1 to 10μM and nitrite production by LPS-stimulated macrophages at a concentration of 10μM. In contrast, BW245C potentiated the production of TNF-α, a pro-inflammatory cytokine, by LPS-stimulated macrophages at concentrations of 1 to 10μM. These results suggest that PGD2 may modulate macrophage functions related to natural immunity via the DP receptor.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"24 1","pages":"522-529"},"PeriodicalIF":0.0,"publicationDate":"2002-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81822578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-12-20DOI: 10.2330/JORALBIOSCI1965.44.530
T. Miyamoto, M. Mizuno, M. Tamura, M. Kawanami
Bone marrow stromal cells contain multipotent stem cells that differentiate into several types of cells under certain conditions in culture. Previously, we reported that a type I collagen matrix induced the osteoblastic differentiation of rat bone marrow stromal cells in culture. In this study, we performed a three dimensional culture within type I collagen gel to elucidate the relationship between the extracellular matrix of the cell culture system and the osteoblastic differentiation of rat bone marrow stromal cells. We compared the osteoblastic gene expressions of the culture cells; (i) culture in conventional culture dish (2D group), (ii) culture in type I collagen-coated culture dish (COAT group), and (iii) culture in three-dimensional type I collagen gel (3D group). In the 3D group of cell culture, the alkaline phosphatase activities of cells were higher than those of the other groups over 3 weeks of culture. Moreover, bone sialoprotein and osteocalcin mRNA expressions were found to be induced by the three-dimensional type I collagen gel culture of rat bone marrow stromal cells. These results suggested that the three dimensional culture of rat bone marrow cells is a crucial factor for inducing osteoblastic differentiation. 抄 録:高 等 動物 の骨髄 間質 細胞 の なか には,多 分化 能 を有す る間葉 系幹 細胞 が存 在 し,培 養 下 にお いて も,種 々 の 条件 に よ りさま ざまな細胞 に分 化 し うる と考 え られ て いる。 われ われ は,こ れ まで に ラ ッ ト骨 髄 間質細 胞 をI 型 コ ラーゲ ン と ともに培 養 す る ことに よ り,骨 芽細 胞 へ の分 化 が誘 導 され る ことを報告 して きた。本 研 究 で は, 〒060-8586 北海 道札 幌市 北 区北13条 西7丁 目 宮本哲朗ほか:ラ ット骨髄間質細胞のコラーゲンゲル内三次元培養 531 細 胞 の三 次元的配 置 と細 胞分 化 との関連 を明 らかにす る 目的 で,ラ ッ ト骨髄 間質細 胞 を通常 の培 養用 デ ィシ ュに て単層 培養 した群(2D群),I型 コ ラーゲ ンを コー トしたデ ィシ ュにて培養 した群(COAT群)な らび にI型 コ ラー ゲ ンゲル内 にて三 次元培 養 を行 った群(3D群)に て それ ぞれ培 養 し,遺 伝子 発現 を比較検 討 した。3D群 に おい て,ほ か の2群 と比較 して,ア ルカ リホス フ ァターゼ活性 が高 く,骨 シアロプ ロテイ ンお よびオ ステオ カル シ ンの遺 伝子 発現 が誘 導 された 。本研 究 に よ り,ラ ッ ト骨髄 間質細胞 をI型 コラーゲ ンゲル 内で三 次元 的 に培 養 す る ことに よ り,骨 芽 細胞 の分化 が誘 導 され る ことを明 らか に した。
{"title":"Osteoblast-Related Gene Expression of Rat Bone Marrow Cells Induced by Three-dimensional Cell Culture in Type I Collagen Gel","authors":"T. Miyamoto, M. Mizuno, M. Tamura, M. Kawanami","doi":"10.2330/JORALBIOSCI1965.44.530","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.530","url":null,"abstract":"Bone marrow stromal cells contain multipotent stem cells that differentiate into several types of cells under certain conditions in culture. Previously, we reported that a type I collagen matrix induced the osteoblastic differentiation of rat bone marrow stromal cells in culture. In this study, we performed a three dimensional culture within type I collagen gel to elucidate the relationship between the extracellular matrix of the cell culture system and the osteoblastic differentiation of rat bone marrow stromal cells. We compared the osteoblastic gene expressions of the culture cells; (i) culture in conventional culture dish (2D group), (ii) culture in type I collagen-coated culture dish (COAT group), and (iii) culture in three-dimensional type I collagen gel (3D group). In the 3D group of cell culture, the alkaline phosphatase activities of cells were higher than those of the other groups over 3 weeks of culture. Moreover, bone sialoprotein and osteocalcin mRNA expressions were found to be induced by the three-dimensional type I collagen gel culture of rat bone marrow stromal cells. These results suggested that the three dimensional culture of rat bone marrow cells is a crucial factor for inducing osteoblastic differentiation. 抄 録:高 等 動物 の骨髄 間質 細胞 の なか には,多 分化 能 を有す る間葉 系幹 細胞 が存 在 し,培 養 下 にお いて も,種 々 の 条件 に よ りさま ざまな細胞 に分 化 し うる と考 え られ て いる。 われ われ は,こ れ まで に ラ ッ ト骨 髄 間質細 胞 をI 型 コ ラーゲ ン と ともに培 養 す る ことに よ り,骨 芽細 胞 へ の分 化 が誘 導 され る ことを報告 して きた。本 研 究 で は, 〒060-8586 北海 道札 幌市 北 区北13条 西7丁 目 宮本哲朗ほか:ラ ット骨髄間質細胞のコラーゲンゲル内三次元培養 531 細 胞 の三 次元的配 置 と細 胞分 化 との関連 を明 らかにす る 目的 で,ラ ッ ト骨髄 間質細 胞 を通常 の培 養用 デ ィシ ュに て単層 培養 した群(2D群),I型 コ ラーゲ ンを コー トしたデ ィシ ュにて培養 した群(COAT群)な らび にI型 コ ラー ゲ ンゲル内 にて三 次元培 養 を行 った群(3D群)に て それ ぞれ培 養 し,遺 伝子 発現 を比較検 討 した。3D群 に おい て,ほ か の2群 と比較 して,ア ルカ リホス フ ァターゼ活性 が高 く,骨 シアロプ ロテイ ンお よびオ ステオ カル シ ンの遺 伝子 発現 が誘 導 された 。本研 究 に よ り,ラ ッ ト骨髄 間質細胞 をI型 コラーゲ ンゲル 内で三 次元 的 に培 養 す る ことに よ り,骨 芽 細胞 の分化 が誘 導 され る ことを明 らか に した。","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"46 1","pages":"530-540"},"PeriodicalIF":0.0,"publicationDate":"2002-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89956488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-08-20DOI: 10.2330/JORALBIOSCI1965.44.274
Kiyoshi Daito, Y. Azuma, Pao‐Li Wang, S. Shirasu, Mikio Kato, M. Shinohara, M. Daito, K. Ohura
De novo protein synthesis in eukaryotes is mainly controlled at the level of gene transcription by transcription factors in the nucleus. In this communication, the expression and nuclear localization of the Rel/NF-κB family member p65 in nuclear and cytosolic fractions of the brain and retina from streptozotocin (STZ) -injected diabetic rats were determined in order to evaluate the possibility that the transportation of Rel/NF-κB family members from the cytoplasm to the nucleus could play a role in diabetes. Immunoblotting assays with a specific antibody revealed a much higher expression of immunoreactive p65 in nuclear fractions than in cytosolic fractions of the cerebral cortex, hippocampus, midbrain and cerebellum after diabetes induction by STZ administration. In contrast, STZ administration suppressed the p65 nuclear localization detected in the striatum, hypothalamus, medulla-pons and retina. These results suggest that STZ-inlected diabetic rats show differential alterations in the nuclear localization of p65 protein in the brain and retina.
{"title":"Expression and Nuclear Localization of p65 in Brain and Retina of Streptozotocin-induced Diabetic Rats with Hyperglycemia","authors":"Kiyoshi Daito, Y. Azuma, Pao‐Li Wang, S. Shirasu, Mikio Kato, M. Shinohara, M. Daito, K. Ohura","doi":"10.2330/JORALBIOSCI1965.44.274","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.274","url":null,"abstract":"De novo protein synthesis in eukaryotes is mainly controlled at the level of gene transcription by transcription factors in the nucleus. In this communication, the expression and nuclear localization of the Rel/NF-κB family member p65 in nuclear and cytosolic fractions of the brain and retina from streptozotocin (STZ) -injected diabetic rats were determined in order to evaluate the possibility that the transportation of Rel/NF-κB family members from the cytoplasm to the nucleus could play a role in diabetes. Immunoblotting assays with a specific antibody revealed a much higher expression of immunoreactive p65 in nuclear fractions than in cytosolic fractions of the cerebral cortex, hippocampus, midbrain and cerebellum after diabetes induction by STZ administration. In contrast, STZ administration suppressed the p65 nuclear localization detected in the striatum, hypothalamus, medulla-pons and retina. These results suggest that STZ-inlected diabetic rats show differential alterations in the nuclear localization of p65 protein in the brain and retina.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"8 1","pages":"274-281"},"PeriodicalIF":0.0,"publicationDate":"2002-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78126997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2002-08-20DOI: 10.2330/JORALBIOSCI1965.44.265
Yasuhito Yamamoto
{"title":"Cloning and Characterization of the Streptococcus mutans Pyruvate Formate-Lyase Gene (plf) and Pyruvate Formate-Lyase-Activating Enzyme Gene (act)","authors":"Yasuhito Yamamoto","doi":"10.2330/JORALBIOSCI1965.44.265","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.44.265","url":null,"abstract":"","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"123 1","pages":"265-273"},"PeriodicalIF":0.0,"publicationDate":"2002-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82241368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：