Pub Date : 2023-12-04DOI: 10.33647/2074-5982-19-4-56-69
E. I. Katorkina, I. V. Lyagoskin, E. N. Bocharova, M. V. Bolotnikova, A. A. Borzov, N. V. Belskaya, A. E. Lisova, K. V. Ulyanova, N. K. Kudina, N. A. Belyanina, O. M. Strizhakova, A. S. Pershin, Ya. A. Bahareva, P. E. Kargopolova, A. P. Vasiliev, A. A. Kazarov, M. Lykov, R. A. Khamitov
In this work, we set out to compare the immunogenicity of the Gam-COVID-Vac two-component vaccine produced by GENERIUM JSC (Russia) following its intranasal or intramuscular administration of the first or both components to BALB/c mice. The immunogenicity was evaluated according to antigen-specific IgG and IgA antibodies in the blood and bronchoalveolar fluid, the number of antigen-specific IFN-γ-producing T-lymphocytes, the number of antigen-specific IFN-γ-producing CD4 and CD8 T-lymphocytes. Intranasal administration was shown to induce the mucosal immunity, significantly exceeding both qualitatively and quantitatively the effect of intramuscular administration (by the number of animals with antigen-specific IgA antibodies and the titer of antibodies in blood serum and bronchoalveolar lavage). At the same time, intramuscular administration slightly exceeded the effect obtained under intranasal administration in terms of the total number of CD8-IFN-γ-producing lymphocytes.
{"title":"Comparative Immunogenicity of the Gam-COVID-Vac Vaccine under Intranasal and Intramuscular Administration","authors":"E. I. Katorkina, I. V. Lyagoskin, E. N. Bocharova, M. V. Bolotnikova, A. A. Borzov, N. V. Belskaya, A. E. Lisova, K. V. Ulyanova, N. K. Kudina, N. A. Belyanina, O. M. Strizhakova, A. S. Pershin, Ya. A. Bahareva, P. E. Kargopolova, A. P. Vasiliev, A. A. Kazarov, M. Lykov, R. A. Khamitov","doi":"10.33647/2074-5982-19-4-56-69","DOIUrl":"https://doi.org/10.33647/2074-5982-19-4-56-69","url":null,"abstract":"In this work, we set out to compare the immunogenicity of the Gam-COVID-Vac two-component vaccine produced by GENERIUM JSC (Russia) following its intranasal or intramuscular administration of the first or both components to BALB/c mice. The immunogenicity was evaluated according to antigen-specific IgG and IgA antibodies in the blood and bronchoalveolar fluid, the number of antigen-specific IFN-γ-producing T-lymphocytes, the number of antigen-specific IFN-γ-producing CD4 and CD8 T-lymphocytes. Intranasal administration was shown to induce the mucosal immunity, significantly exceeding both qualitatively and quantitatively the effect of intramuscular administration (by the number of animals with antigen-specific IgA antibodies and the titer of antibodies in blood serum and bronchoalveolar lavage). At the same time, intramuscular administration slightly exceeded the effect obtained under intranasal administration in terms of the total number of CD8-IFN-γ-producing lymphocytes.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"47 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138602354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-21DOI: 10.33647/2713-0428-19-3e-110-113
A. Kim, E. B. Shustov
A new derivative of dimethylaminoethanol, butanedioic and trans-butenedioic acids (laboratory code ADK-17) was synthesized at the Organic Chemistry Department of the Saint Petersburg State Chemical and Pharmaceutical University (SPSPU). This promising nootropic and anti-asthmatic agent is planned for use in an oral dosage form. The aim was to calculate the pharmacokinetic parameters of the synthesized compound and to determine its possible biotransformation pathways. Rabbits of the Soviet Chinchilla breed were used as a test system in the preclinical study of the compound pharmacokinetics. In accordance with the designed schedule, blood was taken from the marginal vein of the ear, and urine samples were taken. Quantitative determination in biological media was carried out using an Ultimate 3000 liquid chromatograph with a Q-Exactive mass-selective detector with electrospray ionization in accordance with OFS 1.2.1.100015 (Chromatography) of the State Pharmacopoeia by ultra-performance liquid chromatography–mass spectrometry (UPLC-MS). To study the pharmacokinetics of the ADK-17 preparation, a measurement procedure was developed with a detection limit in biological media ranging from 10-6 to 10-3 mg/ml. The determined harmacokinetic parameters show that the absorption of ADK-17 into the systemic circulation from the gastrointestinal tract occurs at an average rate, with its maximum concentration in blood plasma during the oral route of administration being observed between 60–90 min. The elimination half-life from the systemic circulation comprises 4.3 h. Urinary excretion in the first 4 h proceeds at a high rate; however, the compound is detected in the urine within 3 days and in the blood within 2 days from the moment of administration.
{"title":"Pharmacokinetics of a New Dimethylamineethanol Derivative Administered Orally to Laboratory Animals","authors":"A. Kim, E. B. Shustov","doi":"10.33647/2713-0428-19-3e-110-113","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-110-113","url":null,"abstract":"A new derivative of dimethylaminoethanol, butanedioic and trans-butenedioic acids (laboratory code ADK-17) was synthesized at the Organic Chemistry Department of the Saint Petersburg State Chemical and Pharmaceutical University (SPSPU). This promising nootropic and anti-asthmatic agent is planned for use in an oral dosage form. The aim was to calculate the pharmacokinetic parameters of the synthesized compound and to determine its possible biotransformation pathways. Rabbits of the Soviet Chinchilla breed were used as a test system in the preclinical study of the compound pharmacokinetics. In accordance with the designed schedule, blood was taken from the marginal vein of the ear, and urine samples were taken. Quantitative determination in biological media was carried out using an Ultimate 3000 liquid chromatograph with a Q-Exactive mass-selective detector with electrospray ionization in accordance with OFS 1.2.1.100015 (Chromatography) of the State Pharmacopoeia by ultra-performance liquid chromatography–mass spectrometry (UPLC-MS). To study the pharmacokinetics of the ADK-17 preparation, a measurement procedure was developed with a detection limit in biological media ranging from 10-6 to 10-3 mg/ml. The determined harmacokinetic parameters show that the absorption of ADK-17 into the systemic circulation from the gastrointestinal tract occurs at an average rate, with its maximum concentration in blood plasma during the oral route of administration being observed between 60–90 min. The elimination half-life from the systemic circulation comprises 4.3 h. Urinary excretion in the first 4 h proceeds at a high rate; however, the compound is detected in the urine within 3 days and in the blood within 2 days from the moment of administration.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"25 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139252010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-21DOI: 10.33647/2713-0428-19-3e-124-129
K. Shchepetkova, E. G. Batotsyrenova, E. B. Shustov, V. Kashuro, A. V. Sharabanov, N. P. Ramenskaya
Research into the consequences of human exposure to heavy metals acquires particular significance in the context of life and working age prolongation. Such consequences can be manifested either in the form of selective damage to any organ, organ system or function, or in the form of a general violation of the functions of a number of systems and organs and the organism as a whole, in the form of “disintegration of functions”. In this work, we set out to study the neuroprotective effect of etomerzol fumarate after a subchronic low-dose administration of mercury acetate to Wistar rats. After an intragastric administration of an aqueous solution of mercury acetate at a dose of 4 mg/kg for 30 days, a violation of the learning process was identified in an active avoidance by swimming test. The use of the test substance at a dose of 12.5 mg/kg for 14 days had a positive effect on the long-term memory of animals, contributing to the preservation and reproduction of mercury information received.
{"title":"Influence of Etomersol Fumarate on Cognitive Functions of Rats During Mercury Acetate Poisoning","authors":"K. Shchepetkova, E. G. Batotsyrenova, E. B. Shustov, V. Kashuro, A. V. Sharabanov, N. P. Ramenskaya","doi":"10.33647/2713-0428-19-3e-124-129","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-124-129","url":null,"abstract":"Research into the consequences of human exposure to heavy metals acquires particular significance in the context of life and working age prolongation. Such consequences can be manifested either in the form of selective damage to any organ, organ system or function, or in the form of a general violation of the functions of a number of systems and organs and the organism as a whole, in the form of “disintegration of functions”. In this work, we set out to study the neuroprotective effect of etomerzol fumarate after a subchronic low-dose administration of mercury acetate to Wistar rats. After an intragastric administration of an aqueous solution of mercury acetate at a dose of 4 mg/kg for 30 days, a violation of the learning process was identified in an active avoidance by swimming test. The use of the test substance at a dose of 12.5 mg/kg for 14 days had a positive effect on the long-term memory of animals, contributing to the preservation and reproduction of mercury information received.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"113 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139253208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-21DOI: 10.33647/2713-0428-19-3e-114-118
E. Litus, E. Nemashkalova, A. A. Vologzhannikova, E. Deryusheva
Ligands of human serum albumin (HSA) are capable of modulating its interaction with β-amyloid peptide (Aβ), which is a key factor in the pathogenesis of Alzheimer’s disease (AD). L-thyroxine (L-Tr), a natural HSA ligand, is associated with the pathogenesis of AD according to epidemiological and animal model studies. In this work, we studied the kinetics of Aβ fibril formation in the presence of L-Tr and HSA using a fluorescent test with thioflavin T. L-Tr had no significant effect on the inhibitory effect of HSA on fibril growth. At the same time, L-Tr itself had an inhibitory effect similar to that of HSA. Our data can partially explain the relationship between AD and thyroid pathologies.
{"title":"Effect of L-thyroxine, a Human Serum Albumin Natural Ligand, on the Kinetics of β-amyloid Peptide Fibril Formation","authors":"E. Litus, E. Nemashkalova, A. A. Vologzhannikova, E. Deryusheva","doi":"10.33647/2713-0428-19-3e-114-118","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-114-118","url":null,"abstract":"Ligands of human serum albumin (HSA) are capable of modulating its interaction with β-amyloid peptide (Aβ), which is a key factor in the pathogenesis of Alzheimer’s disease (AD). L-thyroxine (L-Tr), a natural HSA ligand, is associated with the pathogenesis of AD according to epidemiological and animal model studies. In this work, we studied the kinetics of Aβ fibril formation in the presence of L-Tr and HSA using a fluorescent test with thioflavin T. L-Tr had no significant effect on the inhibitory effect of HSA on fibril growth. At the same time, L-Tr itself had an inhibitory effect similar to that of HSA. Our data can partially explain the relationship between AD and thyroid pathologies.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"24 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139252687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-21DOI: 10.33647/2713-0428-19-3e-104-109
P. A. Karalkin, K. G. Kudrin, D. Svyatoslavov, D. Usatov, I. V. Reshetov
This paper presents a technology for creating customized porous titanium implants with bioactive coatings, manufactured using additive technologies. The stages of creating an implant include obtaining primary data from the bone defect area using computed tomography; 3D modeling of the defect area and the corresponding implant; production of a customized implant from titanium alloys using selective laser fusion technology; application of bioactive coatings. The as-created customized implant can have several functional structures. Samples of titanium implants with bioactive coatings were subjected to extensive testing. Mathematical modeling and experiments were used to verify the correspondence of the mechanical properties of the developed structures to natural bone tissue. In vitro tests of the studied samples showed the absence of acute toxicity along with high levels of biocompatibility. In vivo tests of the studied samples on Soviet chinchilla rabbits and Anubis baboon monkeys approved by local ethical committees showed their adequate biomechanical and high osteoinductive properties. The successful results of preclinical studies, as well as toxicological and technical tests in certified laboratories, made it possible to create a registration dossier for state registration of customized porous titanium implants with bioactive coatings, manufactured using additive technologies.
{"title":"Development and Preclinical Trials of Castomized Porous Titanium Implants with Bioactive Coatings in Model Systems","authors":"P. A. Karalkin, K. G. Kudrin, D. Svyatoslavov, D. Usatov, I. V. Reshetov","doi":"10.33647/2713-0428-19-3e-104-109","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-104-109","url":null,"abstract":"This paper presents a technology for creating customized porous titanium implants with bioactive coatings, manufactured using additive technologies. The stages of creating an implant include obtaining primary data from the bone defect area using computed tomography; 3D modeling of the defect area and the corresponding implant; production of a customized implant from titanium alloys using selective laser fusion technology; application of bioactive coatings. The as-created customized implant can have several functional structures. Samples of titanium implants with bioactive coatings were subjected to extensive testing. Mathematical modeling and experiments were used to verify the correspondence of the mechanical properties of the developed structures to natural bone tissue. In vitro tests of the studied samples showed the absence of acute toxicity along with high levels of biocompatibility. In vivo tests of the studied samples on Soviet chinchilla rabbits and Anubis baboon monkeys approved by local ethical committees showed their adequate biomechanical and high osteoinductive properties. The successful results of preclinical studies, as well as toxicological and technical tests in certified laboratories, made it possible to create a registration dossier for state registration of customized porous titanium implants with bioactive coatings, manufactured using additive technologies.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139253188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-21DOI: 10.33647/2713-0428-19-3e-130-135
O. M. Kudelina, E. Gantsgorn, Yu. S. Maklyakov, D. L. Tkachenko, L. A. Avakyan, P. A. Chubyreva, Y. G. Amirova, Yu. A. Karpova, A. N. Dulskaya, I. B. Meshi
Aquagenic urticaria is one of the rarest forms of allergic reaction in the body. Aquagenic urticaria is clinically manifested by itching and urticarial rashes 5–10 min after contact with water. The signs disappear within 30–60 min. The standard therapy regimen includes the administration of second-generation H1-histamine receptor blockers and barrier creams with ceramides. In recent years, the use of monoclonal antibody therapy (omalizumab) has been considered in cases where antihistamines are ineffective.
{"title":"Clinical Course and Therapy Methods of Aquagenic Urticaria","authors":"O. M. Kudelina, E. Gantsgorn, Yu. S. Maklyakov, D. L. Tkachenko, L. A. Avakyan, P. A. Chubyreva, Y. G. Amirova, Yu. A. Karpova, A. N. Dulskaya, I. B. Meshi","doi":"10.33647/2713-0428-19-3e-130-135","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-130-135","url":null,"abstract":"Aquagenic urticaria is one of the rarest forms of allergic reaction in the body. Aquagenic urticaria is clinically manifested by itching and urticarial rashes 5–10 min after contact with water. The signs disappear within 30–60 min. The standard therapy regimen includes the administration of second-generation H1-histamine receptor blockers and barrier creams with ceramides. In recent years, the use of monoclonal antibody therapy (omalizumab) has been considered in cases where antihistamines are ineffective.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"68 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139254607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-21DOI: 10.33647/2713-0428-19-3e-119-123
A. Ovcharova, N. V. Belova
Probiotics are microbial preparations, which have a positive effect on the composition of the intestinal microflora and the immune system of animals. These preparations are widely used for correcting and preventing violations of the microflora of the gastrointestinal tract. Lactobacilli play a special role in the formation of normal microflora. They exhibit a pronounced antagonistic activity against pathogenic and opportunistic microorganisms, as well as possess a number of other positive effects. This study is aimed at determining the effect of new probiotic strains of L. reuteri on the indicators of nonspecific resistance, hematological and biochemical parameters of laboratory rabbits. The studied strains were found to have a pronounced positive effect on the indicators of nonspecific resistance. Thus, compared to the control, the indicators of phagocytic activity and bactericidal activity in the experimental group were by 22% and 32% higher, respectively. The content of hemoglobin, erythrocytes and total protein in the blood serum also increased.
益生菌是一种微生物制剂，对动物肠道微生物区系的组成和免疫系统有积极作用。这些制剂被广泛用于纠正和预防胃肠道微生物菌群失调。乳酸菌在正常微生物区系的形成过程中发挥着特殊作用。乳酸菌对病原微生物和机会性微生物具有明显的拮抗活性，并具有许多其他积极作用。本研究旨在确定新的 L. reuteri 益生菌株对实验兔的非特异性抵抗力指标、血液学和生化指标的影响。研究发现，所研究的菌株对非特异性抵抗力指标有明显的积极影响。因此，与对照组相比，实验组的吞噬活性和杀菌活性指标分别提高了 22% 和 32%。血清中的血红蛋白、红细胞和总蛋白含量也有所增加。
{"title":"The effect of L. reuteri on Nonspecific Resistance and Hematological Parameters of Laboratory Animals","authors":"A. Ovcharova, N. V. Belova","doi":"10.33647/2713-0428-19-3e-119-123","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-119-123","url":null,"abstract":"Probiotics are microbial preparations, which have a positive effect on the composition of the intestinal microflora and the immune system of animals. These preparations are widely used for correcting and preventing violations of the microflora of the gastrointestinal tract. Lactobacilli play a special role in the formation of normal microflora. They exhibit a pronounced antagonistic activity against pathogenic and opportunistic microorganisms, as well as possess a number of other positive effects. This study is aimed at determining the effect of new probiotic strains of L. reuteri on the indicators of nonspecific resistance, hematological and biochemical parameters of laboratory rabbits. The studied strains were found to have a pronounced positive effect on the indicators of nonspecific resistance. Thus, compared to the control, the indicators of phagocytic activity and bactericidal activity in the experimental group were by 22% and 32% higher, respectively. The content of hemoglobin, erythrocytes and total protein in the blood serum also increased.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"104 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139253110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-21DOI: 10.33647/2713-0428-19-3e-99-103
A. Ivkina, D. Ivkin, E. B. Shustov, M. N. Povydysh
A comparative evaluation of the effectiveness of dry alcoholic extracts of Dioscorea from pharmacopoeial raw materials and cell cultures was carried out on a model of combined exposure to hypoxic and temperature factors and swimming of laboratory animals (mice) with a load of 5% of body weight. Dioscorea preparations had a pronounced thermoprotective effect and increased physical performance; however, the aftereffect was confirmed only for the cell culture extract.
{"title":"Influence of Dioscorea Extracts on Physical Load Tolerance in the Setting of Hypoxic and Temperature Factors","authors":"A. Ivkina, D. Ivkin, E. B. Shustov, M. N. Povydysh","doi":"10.33647/2713-0428-19-3e-99-103","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-99-103","url":null,"abstract":"A comparative evaluation of the effectiveness of dry alcoholic extracts of Dioscorea from pharmacopoeial raw materials and cell cultures was carried out on a model of combined exposure to hypoxic and temperature factors and swimming of laboratory animals (mice) with a load of 5% of body weight. Dioscorea preparations had a pronounced thermoprotective effect and increased physical performance; however, the aftereffect was confirmed only for the cell culture extract.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"20 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139254340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-20DOI: 10.33647/2713-0428-19-3e-90-94
S. A. Zozulya, O. Sokolov, N. Kost, N. V. Baimeeva, A. K. Dadayan, Yu. A. Zolotarev
In this work, we investigate the anti-inflammatory effect of the HLDF-6-H peptide (Thr-Gly-Glu-HseHis-Arg) in a model of the presymptomatic stage of Parkinson’s disease (PD). This peptide is a bioactive fragment of the HLDF leukocyte differentiation factor. The presymptomatic stage of PD was modelled by introducing moderate doses of MPTP toxin to C57Bl/6 mice. HLDF-6-H was administered intranasally at a dose of 300 μg/kg daily for three weeks. The severity of depression was determined by the time of immobilization in a Porsolt test. The serum levels of 10 steroids were determined using MS analysis. The activity of inflammatory markers leukocyte elastase (LE) and α1-proteinase inhibitor (α1-PI) was determined using kinetic methods. The development of an inflammatory process under the influence of MPTP is evidenced by an increase in the activity of α1-PI; the anti-inflammatory effect of the peptide is manifested in a decreased activity of LE and α1-PI. At the same time, the immobilization time of mice in a Porsolt test positively correlated with α1-PI and LE, which may indicate the role of inflammation in the development of depression. A positive correlation between estradiol and LE was found in the general sample of mice. In the group of mice treated with the peptide, α1-PI correlated positively with corticosterone and negatively with estradiol and androstenedione. It can be assumed that the anti-inflammatory effect of the peptide is associated with the regulation of corticosteroids and sex hormones. Hence, the applied experimental model confirmed the development of a depressive-like syndrome and neuroendocrine disorders in the presymptomatic stage of PD. The long-term use of HLDF-6-H has antidepressant and anti-inflammatory effects.
{"title":"HLDF-6-H Peptide Exhibits an Anti-inflammatory Effect in the Development of Depression in an MPTP Model of Parkinson's Disease","authors":"S. A. Zozulya, O. Sokolov, N. Kost, N. V. Baimeeva, A. K. Dadayan, Yu. A. Zolotarev","doi":"10.33647/2713-0428-19-3e-90-94","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-90-94","url":null,"abstract":"In this work, we investigate the anti-inflammatory effect of the HLDF-6-H peptide (Thr-Gly-Glu-HseHis-Arg) in a model of the presymptomatic stage of Parkinson’s disease (PD). This peptide is a bioactive fragment of the HLDF leukocyte differentiation factor. The presymptomatic stage of PD was modelled by introducing moderate doses of MPTP toxin to C57Bl/6 mice. HLDF-6-H was administered intranasally at a dose of 300 μg/kg daily for three weeks. The severity of depression was determined by the time of immobilization in a Porsolt test. The serum levels of 10 steroids were determined using MS analysis. The activity of inflammatory markers leukocyte elastase (LE) and α1-proteinase inhibitor (α1-PI) was determined using kinetic methods. The development of an inflammatory process under the influence of MPTP is evidenced by an increase in the activity of α1-PI; the anti-inflammatory effect of the peptide is manifested in a decreased activity of LE and α1-PI. At the same time, the immobilization time of mice in a Porsolt test positively correlated with α1-PI and LE, which may indicate the role of inflammation in the development of depression. A positive correlation between estradiol and LE was found in the general sample of mice. In the group of mice treated with the peptide, α1-PI correlated positively with corticosterone and negatively with estradiol and androstenedione. It can be assumed that the anti-inflammatory effect of the peptide is associated with the regulation of corticosteroids and sex hormones. Hence, the applied experimental model confirmed the development of a depressive-like syndrome and neuroendocrine disorders in the presymptomatic stage of PD. The long-term use of HLDF-6-H has antidepressant and anti-inflammatory effects.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"57 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139255971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-20DOI: 10.33647/2713-0428-19-3e-85-89
O. V. Buyuklinskayа, S. M. Napalkova, E. D. Kubasova, D. S. Makarova
Despite the diversity of modern treatment methods, infertility rates remain high both in Russian and globally. In the present experimental study, we study the effect of a nanopreparation based on a selenoxanthene derivative and cyclodextrin on the reproduction of white outbred rats (females) after the administration of mifepristone. Selenoxanthene at a dose of 0.35 mg/kg and 0.7 mg/kg intragastrically for 5 days increased the reproductive activity of animals.
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