Pub Date : 2023-11-20DOI: 10.33647/2713-0428-19-3e-36-42
O. B. Zhukova, E. Koloskova
Hormonal preparation of female egg donors is one of the first stages in obtaining genetically modified animals by microinjected zygote transplantation. A large number of embryos synchronized in development can be obtained by a superovulation procedure based on treating female animals with gonadotropins. As a rule, drugs from different manufacturers differ markedly in terms of their efficacy. In the context of sanctions imposed on the Russian Federation, attention should be paid to domestic hormonal drugs, thus substantiating their objective assessment. It was shown that different batches of the FSH-SUPER drug, used mainly for hormonal treatment of farm animals, differed in stimulating effect on superovulation in female mice. At the same time, the efficacy of this drug surpassed that of Follimag (Mosagrogen, Russia).
{"title":"Response of Mouse Ovaries to Stimulation witch ihe FSH-SU-PER Drug","authors":"O. B. Zhukova, E. Koloskova","doi":"10.33647/2713-0428-19-3e-36-42","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-36-42","url":null,"abstract":"Hormonal preparation of female egg donors is one of the first stages in obtaining genetically modified animals by microinjected zygote transplantation. A large number of embryos synchronized in development can be obtained by a superovulation procedure based on treating female animals with gonadotropins. As a rule, drugs from different manufacturers differ markedly in terms of their efficacy. In the context of sanctions imposed on the Russian Federation, attention should be paid to domestic hormonal drugs, thus substantiating their objective assessment. It was shown that different batches of the FSH-SUPER drug, used mainly for hormonal treatment of farm animals, differed in stimulating effect on superovulation in female mice. At the same time, the efficacy of this drug surpassed that of Follimag (Mosagrogen, Russia).","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"44 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139259049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-20DOI: 10.33647/2713-0428-19-3e-71-76
N. S. Tropskaya, E. A. Kislyаkova, I. G. Vilkova, Yu. V. Gurman, O. S. Kislitsyna, Ye. N. Borodina, T. V. Chernen'kaya, T. S. Popova
A scheme for correcting intestinal motor disorders and microbiocenosis is proposed. Experiments on rats included a sequential jejunum administration of pharmaconutrient enteral nutrition (Intestamine, containing glutamine and tributyrin) and inulin, a prebiotic preparation. The application of this scheme in the postoperative period in the setting of endotoxemia prevents excessive bacterial growth in the jejunum, stimulates the appearance of propulsive peristalsis, eliminates pathological motility patterns and promotes early recovery of coordinated peristalsis of the small intestine.
{"title":"Experimental Study of the Efficacy of Pharmaconutrient Enteral Nutrition and Inulin in the Correction of Intestinal Motor Disorders and Microbiocenosis in the Postoperative Period Under Endotoxemia Conditions","authors":"N. S. Tropskaya, E. A. Kislyаkova, I. G. Vilkova, Yu. V. Gurman, O. S. Kislitsyna, Ye. N. Borodina, T. V. Chernen'kaya, T. S. Popova","doi":"10.33647/2713-0428-19-3e-71-76","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-71-76","url":null,"abstract":"A scheme for correcting intestinal motor disorders and microbiocenosis is proposed. Experiments on rats included a sequential jejunum administration of pharmaconutrient enteral nutrition (Intestamine, containing glutamine and tributyrin) and inulin, a prebiotic preparation. The application of this scheme in the postoperative period in the setting of endotoxemia prevents excessive bacterial growth in the jejunum, stimulates the appearance of propulsive peristalsis, eliminates pathological motility patterns and promotes early recovery of coordinated peristalsis of the small intestine.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"66 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139259178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-20DOI: 10.33647/2713-0428-19-3e-43-46
T. A. Kudryashov, M. V. Trunilina, V. V. Bykov, I. S. Boldaevsky, A. S. Sokolov, Yu. S. Lapteva
N-terminal acetyltransferases (NATs) of bacteria acetylate the alpha-amino group in amino acids and proteins, participate in the biosynthesis of lantibiotics, and inactivate a number of antibiotics. NATs are used in biotechnology for targeted acetylation of recombinant proteins and peptides. In this regard, the search for NATs that differ in terms of substrate specificity and are also capable of functioning in the reaction at elevated temperatures, a wide pH range, etc., seems relevant. In this work, we develop specific characteristics and a search algorithm for the identification of N-terminal acetyltransferases using the Thermus thermophilus thermophilic bacterium as an example. Out of 14 Abs annotated in the genome, we selected six «putative» NATs. Some of the genes encoding the selected NATs were successfully cloned, generated, and purified from E. coli cells. The specific enzymatic activity of a number of enzymes was confirmed.
{"title":"Development of an Algorithm for Identification of N-terminal Acetyltransferases and Verification of Their Functional Activity","authors":"T. A. Kudryashov, M. V. Trunilina, V. V. Bykov, I. S. Boldaevsky, A. S. Sokolov, Yu. S. Lapteva","doi":"10.33647/2713-0428-19-3e-43-46","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-43-46","url":null,"abstract":"N-terminal acetyltransferases (NATs) of bacteria acetylate the alpha-amino group in amino acids and proteins, participate in the biosynthesis of lantibiotics, and inactivate a number of antibiotics. NATs are used in biotechnology for targeted acetylation of recombinant proteins and peptides. In this regard, the search for NATs that differ in terms of substrate specificity and are also capable of functioning in the reaction at elevated temperatures, a wide pH range, etc., seems relevant. In this work, we develop specific characteristics and a search algorithm for the identification of N-terminal acetyltransferases using the Thermus thermophilus thermophilic bacterium as an example. Out of 14 Abs annotated in the genome, we selected six «putative» NATs. Some of the genes encoding the selected NATs were successfully cloned, generated, and purified from E. coli cells. The specific enzymatic activity of a number of enzymes was confirmed.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"44 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139255070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-20DOI: 10.33647/2713-0428-19-3e-81-84
A. A. Bondarenko, A. S. Melehova, A. Y. Bespalov, T. L. Gorchakova, L. I. Prokopenko
Daphnia magna Straus was used as an experimental zoohydrobiont model for primary screening of compounds of the valproic acid aminoether group with anticonvulsant and cholinoblocking activity. On this basis, the most effective compounds for further trials in warm-blooded animals were selected. The selection criterion included their minimum effective concentration for the prevention of atypical motor hyperactivity induced by phenylcarbamate.
{"title":"Daphnia magna Straus as a Test Object for Screening Cholinergic Pharmacological Substances","authors":"A. A. Bondarenko, A. S. Melehova, A. Y. Bespalov, T. L. Gorchakova, L. I. Prokopenko","doi":"10.33647/2713-0428-19-3e-81-84","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-81-84","url":null,"abstract":"Daphnia magna Straus was used as an experimental zoohydrobiont model for primary screening of compounds of the valproic acid aminoether group with anticonvulsant and cholinoblocking activity. On this basis, the most effective compounds for further trials in warm-blooded animals were selected. The selection criterion included their minimum effective concentration for the prevention of atypical motor hyperactivity induced by phenylcarbamate.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"2 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139257676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-20DOI: 10.33647/2713-0428-19-3e-47-51
Yu. S. Lapteva, V. V. Bykov, M. V. Trunilina, I. S. Boldaevsky, T. A. Kudryashov, А. A. Vologzhannikova, A. S. Sokolov
Cleavage of the N-terminal initiating methionine (Met1) is a critical coand post-translational modification affecting 50–70% of cellular proteins. During the production of recombinant proteins in the heterologous system of E. coli expression, Met1 cleavage often fails to occur, which leads to heterogeneity of the preparations obtained, changes in their activity and stability. This problem can be solved by treating recombinant proteins in vitro with a specific enzyme, methionine aminopeptidase (MAP). Currently available MAPs exhibit limited specificities and reaction conditions. We cloned a MAP from a hyperthermophilic bacterium, developed a method for enzyme purification, and studied a number of physicochemical properties. The new MAP enzyme is resistant to elevated temperatures. The MAP maintains a stable native state in a pH range from 3 to 11 units. The novel MAP enzyme can be used to remove N-terminal Met1 from recombinant proteins in vitro over a wide pH range and at elevated temperatures.
{"title":"Obtaining Overstable Methionine Aminopeptidase for the Removal of Methionine From Recombinant Proteins","authors":"Yu. S. Lapteva, V. V. Bykov, M. V. Trunilina, I. S. Boldaevsky, T. A. Kudryashov, А. A. Vologzhannikova, A. S. Sokolov","doi":"10.33647/2713-0428-19-3e-47-51","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-47-51","url":null,"abstract":"Cleavage of the N-terminal initiating methionine (Met1) is a critical coand post-translational modification affecting 50–70% of cellular proteins. During the production of recombinant proteins in the heterologous system of E. coli expression, Met1 cleavage often fails to occur, which leads to heterogeneity of the preparations obtained, changes in their activity and stability. This problem can be solved by treating recombinant proteins in vitro with a specific enzyme, methionine aminopeptidase (MAP). Currently available MAPs exhibit limited specificities and reaction conditions. We cloned a MAP from a hyperthermophilic bacterium, developed a method for enzyme purification, and studied a number of physicochemical properties. The new MAP enzyme is resistant to elevated temperatures. The MAP maintains a stable native state in a pH range from 3 to 11 units. The novel MAP enzyme can be used to remove N-terminal Met1 from recombinant proteins in vitro over a wide pH range and at elevated temperatures.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"33 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139256284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-20DOI: 10.33647/2713-0428-19-3e-52-58
S. A. Machulin, T. Astrelina, D. Usupzhanova, A. O. Zavialov, T. Malivanova, А. I. Golovkova, I. Kobzeva, Y. Suchkova, V. Brunchukov, A. Rastorgueva, V. Nikitina, E. E. Lomonosova, O. G. Mihadarkina, A. Samoilov
This article describes the process of developing a tissue-engineered structure that meets the biocompatibility and biodegradation parameters necessary for replacing cartilage tissue defects. The study was carried out using 3D bioprinting technology, which represents a promising research direction in the biomedical field. It is known that, due to the specifics of its structure, cartilage tissue is not capable of complete regeneration of damage. The methods currently used for treating arthrosis are associated with a number of limitations and disadvantages, which makes research aimed at developing alternative methods for arthrosis treatment particularly relevant. The development of tissue-engineered structures by 3D bioprinting requires the materials not only certified for medical use but also exhibiting biocompatibility and biodegradation properties. Polylactide (PLA) and sodium alginate satisfy the above requirements; moreover, their availability and economic affordability make them one of the most popular materials for 3D bioprinting.
{"title":"3D Printing of a Tissue-Engineered Structure Intended to Replace Cartilage Defects","authors":"S. A. Machulin, T. Astrelina, D. Usupzhanova, A. O. Zavialov, T. Malivanova, А. I. Golovkova, I. Kobzeva, Y. Suchkova, V. Brunchukov, A. Rastorgueva, V. Nikitina, E. E. Lomonosova, O. G. Mihadarkina, A. Samoilov","doi":"10.33647/2713-0428-19-3e-52-58","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-52-58","url":null,"abstract":"This article describes the process of developing a tissue-engineered structure that meets the biocompatibility and biodegradation parameters necessary for replacing cartilage tissue defects. The study was carried out using 3D bioprinting technology, which represents a promising research direction in the biomedical field. It is known that, due to the specifics of its structure, cartilage tissue is not capable of complete regeneration of damage. The methods currently used for treating arthrosis are associated with a number of limitations and disadvantages, which makes research aimed at developing alternative methods for arthrosis treatment particularly relevant. The development of tissue-engineered structures by 3D bioprinting requires the materials not only certified for medical use but also exhibiting biocompatibility and biodegradation properties. Polylactide (PLA) and sodium alginate satisfy the above requirements; moreover, their availability and economic affordability make them one of the most popular materials for 3D bioprinting.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"42 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139258056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-20DOI: 10.33647/2713-0428-19-3e-77-80
M. Shevelyova, E. Nemashkalova, E. Deryusheva
In this work, we studied the effect of heparin on the binding process of VEGF-A165, the most common and important growth factor, and the monoclonal antibody bevacizumab by bio-layer interferometry. A complex of the full-length bevacizumab antibody with VEGF-A165 was modeled. The data obtained can be used in therapy with this antibody, as well as in the development of other therapeutic antibodies with maximum target specificity under various conditions.
{"title":"Effect of Heparin on Antigen-Antibody Interaction in a Model System of the Bevacizumab Therapeutic Antibody And its Target VEGF-А165","authors":"M. Shevelyova, E. Nemashkalova, E. Deryusheva","doi":"10.33647/2713-0428-19-3e-77-80","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-77-80","url":null,"abstract":"In this work, we studied the effect of heparin on the binding process of VEGF-A165, the most common and important growth factor, and the monoclonal antibody bevacizumab by bio-layer interferometry. A complex of the full-length bevacizumab antibody with VEGF-A165 was modeled. The data obtained can be used in therapy with this antibody, as well as in the development of other therapeutic antibodies with maximum target specificity under various conditions.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"30 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139255945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-20DOI: 10.33647/2713-0428-19-3e-59-65
O. Stepanova, R. A. Klesov, Kh. Kh. Semenov, I. A. Pomytkin, V. Karkischenko
We studied the effect of infrared radiation (IR) course exposure on pathological changes in the tissues of C57BL/KsJYLeprdb/+ (db/db) mutant mice with type 2 diabetes mellitus using a LASMA ST device. This apparatus allows simultaneous non-invasive monitoring of blood and lymph microcirculation, as well as determining the activity of mitochondrial oxidative coenzymes in tissues before and after IR therapy. IR waves (690 nm) were found to have a positive therapeutic effect on the functional state of db/db mice. This included a rapid healing of skin wounds caused by maceration and prolonged normalization of oxidative metabolism parameters (NADH, FAD, POM) in body tissues. IR therapy has a cumulative effect, leading to a 2.3–2.5-fold increase in life expectancy and life quality, compared to the control group.
{"title":"Effect of Infrared Radiation on the Regulation of Oxidative Netabolism in C57BL/KsJYLeprdb/+ Mice","authors":"O. Stepanova, R. A. Klesov, Kh. Kh. Semenov, I. A. Pomytkin, V. Karkischenko","doi":"10.33647/2713-0428-19-3e-59-65","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-59-65","url":null,"abstract":"We studied the effect of infrared radiation (IR) course exposure on pathological changes in the tissues of C57BL/KsJYLeprdb/+ (db/db) mutant mice with type 2 diabetes mellitus using a LASMA ST device. This apparatus allows simultaneous non-invasive monitoring of blood and lymph microcirculation, as well as determining the activity of mitochondrial oxidative coenzymes in tissues before and after IR therapy. IR waves (690 nm) were found to have a positive therapeutic effect on the functional state of db/db mice. This included a rapid healing of skin wounds caused by maceration and prolonged normalization of oxidative metabolism parameters (NADH, FAD, POM) in body tissues. IR therapy has a cumulative effect, leading to a 2.3–2.5-fold increase in life expectancy and life quality, compared to the control group.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139257884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-20DOI: 10.33647/2713-0428-19-3e-31-35
A. A. Deltsov, S. V. Pozyabin
Systemic response to stress, aimed at eliminating or reducing stress, is accompanied by changes in behavioral, vegetative, motor, sensory, cognitive, and other bodily functions. Stress behavior is an integral part of general behavior. In this work, we set out to study the behavioral activity of rats in an open field test following administration of various lithium salts. The conducted experiments showed that a course application of organic lithium salts improves the motivational characteristics of behavior and the appearance of rats, significantly increases the tentative research behavior of experimental animals, and reduces the level of anxiety, fear, and uncertainty, which can be characterized as an adaptive-stimulating effect. At the same time, the drug demonstrates a cumulative effect. The anabolic effect of lithium orotate is particularly pronounced, which makes it possible to increase body weight gains in the setting of stress of various etiologies.
{"title":"Comparative Analysis of the Stress Protective Effect of Lithium Salts in an Open Field Test","authors":"A. A. Deltsov, S. V. Pozyabin","doi":"10.33647/2713-0428-19-3e-31-35","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-31-35","url":null,"abstract":"Systemic response to stress, aimed at eliminating or reducing stress, is accompanied by changes in behavioral, vegetative, motor, sensory, cognitive, and other bodily functions. Stress behavior is an integral part of general behavior. In this work, we set out to study the behavioral activity of rats in an open field test following administration of various lithium salts. The conducted experiments showed that a course application of organic lithium salts improves the motivational characteristics of behavior and the appearance of rats, significantly increases the tentative research behavior of experimental animals, and reduces the level of anxiety, fear, and uncertainty, which can be characterized as an adaptive-stimulating effect. At the same time, the drug demonstrates a cumulative effect. The anabolic effect of lithium orotate is particularly pronounced, which makes it possible to increase body weight gains in the setting of stress of various etiologies.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"73 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139255688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-20DOI: 10.33647/2713-0428-19-3e-66-70
N. S. Strelnikova, O. Rozanova, H. N. Smirnova, T. A. Belyakova, A. V. Smirnov
We study the induction patterns of the solid form of Ehrlich ascitic carcinoma (EAC) in mice in the short(1 month) and long-term (18 months) periods after inoculation of ascitic cells irradiated ex vivo with a proton beam at doses of 30, 60, 90, 120, and 150 Gr. The growth rate of solid tumors after inoculation of irradiated cells ex vivo coincided with the growth of tumors in the control group. The frequency of tumor induction in mice transplanted with EAC cells was dose-dependent and decreased from 80% to 0% along with an increase in radiation dose. Thus, using the ex vivo model of tumor cell irradiation followed by in vivo grafting allowed us to determine the parameters of the antitumor effect of new proton therapy regimens.
{"title":"Efficacy of High Doses of Protons on the Induction of Solid Tumors in Mice by Ex vivo Irradiation of Ehrlich Ascitic Carcinoma Cells","authors":"N. S. Strelnikova, O. Rozanova, H. N. Smirnova, T. A. Belyakova, A. V. Smirnov","doi":"10.33647/2713-0428-19-3e-66-70","DOIUrl":"https://doi.org/10.33647/2713-0428-19-3e-66-70","url":null,"abstract":"We study the induction patterns of the solid form of Ehrlich ascitic carcinoma (EAC) in mice in the short(1 month) and long-term (18 months) periods after inoculation of ascitic cells irradiated ex vivo with a proton beam at doses of 30, 60, 90, 120, and 150 Gr. The growth rate of solid tumors after inoculation of irradiated cells ex vivo coincided with the growth of tumors in the control group. The frequency of tumor induction in mice transplanted with EAC cells was dose-dependent and decreased from 80% to 0% along with an increase in radiation dose. Thus, using the ex vivo model of tumor cell irradiation followed by in vivo grafting allowed us to determine the parameters of the antitumor effect of new proton therapy regimens.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"21 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139258492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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