Pub Date : 2022-12-12DOI: 10.33647/2074-5982-18-4-112-122
V. Kokhan, P. Anokhin, E. P. Pakhlova, N. Sarycheva, I. Shamakina
Disruption of normal epigenetic reprogramming during the prenatal period under the influence of exogenous factors affects fetus development and adult phenotype formation. The mechanisms through which determinants, such as maternal alcohol intake, contribute to the formation of an alcohol-vulnerable phenotype later in life still remain unclear. In this paper, we suggest that alteration in the reinforcing properties of ethanol in prenatally alcohol-exposed subjects may be associated with transcriptional dysregulation of the brain opioid receptor genes. We compared voluntary alcohol intake and levels of mRNA coding for μ- (MOP) and κ-opioid (KOP) receptors in the mesolimbic areas of adult male offspring of the female Wistar rats having received 10% ethanol as the only source of liquid throughout pregnancy or water (control). We found that prenatally alcohol exposed rats had higher alcohol preference on PND60 (free-choice paradigm) and lower mRNA expression for both MOP and KOP in the midbrain compared to the control. This suggests a potential link between prenatal alcohol, dysfunction of the brain opiate system and adult vulnerability for alcohol use disorder.
{"title":"Impaired Expression of Mu- and Kappa-Opioid Receptor mRNA in the Midbrain of Rats with Prenatal Alcohol Intoxication","authors":"V. Kokhan, P. Anokhin, E. P. Pakhlova, N. Sarycheva, I. Shamakina","doi":"10.33647/2074-5982-18-4-112-122","DOIUrl":"https://doi.org/10.33647/2074-5982-18-4-112-122","url":null,"abstract":"Disruption of normal epigenetic reprogramming during the prenatal period under the influence of exogenous factors affects fetus development and adult phenotype formation. The mechanisms through which determinants, such as maternal alcohol intake, contribute to the formation of an alcohol-vulnerable phenotype later in life still remain unclear. In this paper, we suggest that alteration in the reinforcing properties of ethanol in prenatally alcohol-exposed subjects may be associated with transcriptional dysregulation of the brain opioid receptor genes. We compared voluntary alcohol intake and levels of mRNA coding for μ- (MOP) and κ-opioid (KOP) receptors in the mesolimbic areas of adult male offspring of the female Wistar rats having received 10% ethanol as the only source of liquid throughout pregnancy or water (control). We found that prenatally alcohol exposed rats had higher alcohol preference on PND60 (free-choice paradigm) and lower mRNA expression for both MOP and KOP in the midbrain compared to the control. This suggests a potential link between prenatal alcohol, dysfunction of the brain opiate system and adult vulnerability for alcohol use disorder.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81502859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-12DOI: 10.33647/2074-5982-18-4-94-101
M. Sultanly
This paper investigates the state of axodendritic (ADS) and axosomatic (ASS) synapses in orbitofrontal cortex (OFC) layers I-IV of adult white laboratory rats associated with balanced and low-protein food after acute sound exposure. Experiments were performed on 64 white non-linear sexually mature laboratory male rats weighing 180–230 g (eight intact, 56 experimental). After a continuous call with an intensity of 120 db for 120 seconds, 56 rats were divided into two groups: those receiving balanced (control – 28) and low-protein food (basic – 28). Each of these two groups was divided into two subgroups: stress-resistant (12 animals in each) and stress-unstable animals (16 animals in each). Water intake was unlimited. The animals were removed from the experiment on the 10th, 20th, 30th, and 40th day after sound exposure. Along with histological and immunohistochemical analysis, the samples of OFC layers I–IV were studied by transmission electron microscopy. Electron microscopic changes in the structure of ADS and ASS were found in all OFC layers. Violations of the fine structure of both the presynaptic and postsynaptic poles were noted. The maximum severity of ultrastructural changes was observed in the ADS of the surface (I, molecular), outer granular (II), pyramidal (III), and inner granular (IV) OFC layers. ASS disorganization was noted mainly in contacts, the postsynaptic pole of which was formed by the bodies of small pyramidal neurons of layer III, as well as pyramidal and stellate neurons of layer IV of the OFC. During all periods of observation, violations of fine organization were most pronounced in stress-unstable animals of the main experimental group, especially on the 10th and 20th day after exposure. Acute sound exposure initiates disturbances in the fine organization of axodendritic and axosomatic synapses in OFC layers I–IV of white laboratory rats. Focal destruction of a part of the noted synapses in rats with a low-protein diet after acute auditory stress is irreversible.
{"title":"Ultrastructural Characteristics of Axodendritic and Axosomatic Synapses in the Orbitofrontal Cortex of White Laboratory Rats Associated with Low-Protein Food after Acute Sound Exposure","authors":"M. Sultanly","doi":"10.33647/2074-5982-18-4-94-101","DOIUrl":"https://doi.org/10.33647/2074-5982-18-4-94-101","url":null,"abstract":"This paper investigates the state of axodendritic (ADS) and axosomatic (ASS) synapses in orbitofrontal cortex (OFC) layers I-IV of adult white laboratory rats associated with balanced and low-protein food after acute sound exposure. Experiments were performed on 64 white non-linear sexually mature laboratory male rats weighing 180–230 g (eight intact, 56 experimental). After a continuous call with an intensity of 120 db for 120 seconds, 56 rats were divided into two groups: those receiving balanced (control – 28) and low-protein food (basic – 28). Each of these two groups was divided into two subgroups: stress-resistant (12 animals in each) and stress-unstable animals (16 animals in each). Water intake was unlimited. The animals were removed from the experiment on the 10th, 20th, 30th, and 40th day after sound exposure. Along with histological and immunohistochemical analysis, the samples of OFC layers I–IV were studied by transmission electron microscopy. Electron microscopic changes in the structure of ADS and ASS were found in all OFC layers. Violations of the fine structure of both the presynaptic and postsynaptic poles were noted. The maximum severity of ultrastructural changes was observed in the ADS of the surface (I, molecular), outer granular (II), pyramidal (III), and inner granular (IV) OFC layers. ASS disorganization was noted mainly in contacts, the postsynaptic pole of which was formed by the bodies of small pyramidal neurons of layer III, as well as pyramidal and stellate neurons of layer IV of the OFC. During all periods of observation, violations of fine organization were most pronounced in stress-unstable animals of the main experimental group, especially on the 10th and 20th day after exposure. Acute sound exposure initiates disturbances in the fine organization of axodendritic and axosomatic synapses in OFC layers I–IV of white laboratory rats. Focal destruction of a part of the noted synapses in rats with a low-protein diet after acute auditory stress is irreversible.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87148093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-11DOI: 10.33647/2074-5982-18-4-63-73
V. V. Vorobieva, O. S. Levchenkova, P. Shabanov
The effect of nifedipine, a calcium channel blocker (CCB), at a dose of 7.5 mg/kg on the energy metabolism of rabbit cardiomyocytes was evaluated in a vibration-mediated model of cellular hypoxia (56 sessions of vibration 44 Hz, amplitude 0.5 mm). The energy metabolism of native heart mitochondria in a 30% tissue homogenate was assessed using a polarographic method, by recording the rate of oxygen uptake by mitochondria at 37°C in 1 ml of a saline incubation medium, equilibrated with atmospheric oxygen. In the animals exposed to vibration against the background of CCB, the rate of endogenous respiration (Ve) remained at the level of intact animals, with the amytal sensitivity increasing by 39% (p<0.05) and the malonate sensitivity decreasing by 40% (p<0.05). The malate oxidase activity at rest increased, and hyperactivation of the endogenous succinic acid oxidation system decreased as compared to the indices of the animals exposed to vibration without pharmacological protection. The observed results indicate the cardioprotective effect of nifedipine, which prevented the development of cardiomyocyte necrosis.
{"title":"Blockade of Rabbit Cardiomyocyte Calcium Channels Restores the Activity of Enzyme-Substrate Complexes of the Respiratory Chain in a Model of Vibration-Mediated Hypoxia","authors":"V. V. Vorobieva, O. S. Levchenkova, P. Shabanov","doi":"10.33647/2074-5982-18-4-63-73","DOIUrl":"https://doi.org/10.33647/2074-5982-18-4-63-73","url":null,"abstract":"The effect of nifedipine, a calcium channel blocker (CCB), at a dose of 7.5 mg/kg on the energy metabolism of rabbit cardiomyocytes was evaluated in a vibration-mediated model of cellular hypoxia (56 sessions of vibration 44 Hz, amplitude 0.5 mm). The energy metabolism of native heart mitochondria in a 30% tissue homogenate was assessed using a polarographic method, by recording the rate of oxygen uptake by mitochondria at 37°C in 1 ml of a saline incubation medium, equilibrated with atmospheric oxygen. In the animals exposed to vibration against the background of CCB, the rate of endogenous respiration (Ve) remained at the level of intact animals, with the amytal sensitivity increasing by 39% (p<0.05) and the malonate sensitivity decreasing by 40% (p<0.05). The malate oxidase activity at rest increased, and hyperactivation of the endogenous succinic acid oxidation system decreased as compared to the indices of the animals exposed to vibration without pharmacological protection. The observed results indicate the cardioprotective effect of nifedipine, which prevented the development of cardiomyocyte necrosis.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81005531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-11DOI: 10.33647/2074-5982-18-4-74-85
O. Rybalkina, T. Razina, E. A. Kiseleva, G. I. Kalinkina, N. V. Isaikina, E. Zueva, V. V. Zhdanov
Mountain ash (Sorbus aucuparia L.) is a prominent representative of phenolic medicinal plants. A widespread and cultivated plant, it has a sufficient raw material base not only of fruits, but also of other parts of the plant (leaves, flowers); it is a promising source of biologically active complexes for the development of new medical drugs. In the work, the content of the main groups of phenolic compounds in plant extracts from fruits, leaves and flowers of S. aucuparia L. was determined. Extracts were obtained using the original technology with acidified 95% ethanol. The content of the sum of phenolic compounds was determined, as well as the content of anthocyanins, flavonoids, phenolic acids, tannins. The effect of these phenol-containing complexes on the development of transplanted tumors (Lewis lung carcinoma, lung cancer-67) and the effectiveness of cyclophosphane treatment were studied. It was revealed that the use of plant complexes leads to a significant inhibition of the development of metastases in the lungs, as well as an increase in the antitumor and anti-metastatic activity of cyclophosphane in combined treatment. The new data obtained are of interest for further study of these phenol-containing complexes in order to create drugs based on them to increase the effectiveness of chemotherapy for malignant neoplasms.
{"title":"Evaluation of the Efficiency of Phenol-Containing Complexes Extracted from Different Parts of Sorbus Aucuparia L. in an Oncological Experiment","authors":"O. Rybalkina, T. Razina, E. A. Kiseleva, G. I. Kalinkina, N. V. Isaikina, E. Zueva, V. V. Zhdanov","doi":"10.33647/2074-5982-18-4-74-85","DOIUrl":"https://doi.org/10.33647/2074-5982-18-4-74-85","url":null,"abstract":"Mountain ash (Sorbus aucuparia L.) is a prominent representative of phenolic medicinal plants. A widespread and cultivated plant, it has a sufficient raw material base not only of fruits, but also of other parts of the plant (leaves, flowers); it is a promising source of biologically active complexes for the development of new medical drugs. In the work, the content of the main groups of phenolic compounds in plant extracts from fruits, leaves and flowers of S. aucuparia L. was determined. Extracts were obtained using the original technology with acidified 95% ethanol. The content of the sum of phenolic compounds was determined, as well as the content of anthocyanins, flavonoids, phenolic acids, tannins. The effect of these phenol-containing complexes on the development of transplanted tumors (Lewis lung carcinoma, lung cancer-67) and the effectiveness of cyclophosphane treatment were studied. It was revealed that the use of plant complexes leads to a significant inhibition of the development of metastases in the lungs, as well as an increase in the antitumor and anti-metastatic activity of cyclophosphane in combined treatment. The new data obtained are of interest for further study of these phenol-containing complexes in order to create drugs based on them to increase the effectiveness of chemotherapy for malignant neoplasms.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88409620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-11DOI: 10.33647/2074-5982-18-4-24-38
А . М . Зубалий, М . С . Нестеров, В . Н . Каркищенко, Дмитрий Вадимович Хвостов, Р . А . Агельдинов, С . В . Огнев, A. Zubalii, M. S. Nesterov, V. Karkischenko, D. Khvostov, R. Ageldinov, Sergey V. Ognev
The content of biologically active substances in the gland secretion of Siberian musk deer obtained in vivo was studied by gas chromatography-mass spectrometry using derivatization by silylation. The musk chemical composition of animals held under natural conditions and in captivity was compared. The food spectrum of Siberian musk deer was found to affect the content of biologically active components in the musk, primarily of androsteroid substances. The results of evaluating the content of musk components exhibiting the most pronounced biological effects are presented. An increase in the diversity of food spectrum was established to improve the qualitative composition of biologically active substances in the musk obtained from the adult males of Siberian musk deer held in captivity.
{"title":"Content of Biologically Active Substances in the Musk of Siberian Musk Deer Depending on Food Spectrum of Animal Nutrition","authors":"А . М . Зубалий, М . С . Нестеров, В . Н . Каркищенко, Дмитрий Вадимович Хвостов, Р . А . Агельдинов, С . В . Огнев, A. Zubalii, M. S. Nesterov, V. Karkischenko, D. Khvostov, R. Ageldinov, Sergey V. Ognev","doi":"10.33647/2074-5982-18-4-24-38","DOIUrl":"https://doi.org/10.33647/2074-5982-18-4-24-38","url":null,"abstract":"The content of biologically active substances in the gland secretion of Siberian musk deer obtained in vivo was studied by gas chromatography-mass spectrometry using derivatization by silylation. The musk chemical composition of animals held under natural conditions and in captivity was compared. The food spectrum of Siberian musk deer was found to affect the content of biologically active components in the musk, primarily of androsteroid substances. The results of evaluating the content of musk components exhibiting the most pronounced biological effects are presented. An increase in the diversity of food spectrum was established to improve the qualitative composition of biologically active substances in the musk obtained from the adult males of Siberian musk deer held in captivity.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"19 35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90255145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-11DOI: 10.33647/2074-5982-18-4-39-47
N. Stankova, O. V. Alimkina, I. A. Pomytkin, V. Karkischenko
Extreme physical exertion (work to failure) was simulated in untrained mini pigs. The results of complete blood tests, lactate measurements and clinical urine analysis revealed variations in the basic parameters of animals similar to those observed in people after extreme exertion.
{"title":"Analysis of Variations in the Basic Indicators of Mini Pigs under Extreme Physical Exertion","authors":"N. Stankova, O. V. Alimkina, I. A. Pomytkin, V. Karkischenko","doi":"10.33647/2074-5982-18-4-39-47","DOIUrl":"https://doi.org/10.33647/2074-5982-18-4-39-47","url":null,"abstract":"Extreme physical exertion (work to failure) was simulated in untrained mini pigs. The results of complete blood tests, lactate measurements and clinical urine analysis revealed variations in the basic parameters of animals similar to those observed in people after extreme exertion.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75184189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-11DOI: 10.33647/2074-5982-18-4-10-23
E. S. Savchenko, N. S. Ogneva, N. Karkischenko
The accumulation of scientific data in the field of pharmacogenetics requires the creation of adequate biomodels to reflect the immunogenetic characteristics of different population groups. We have obtained the ancestors of a new humanized transgenic mouse line carrying the human HLA-A*02:01:01:01 gene, which is characteristic of the Russian population. The new biomodels was created using the pronuclei microinjection method of a linearized fragment of genetically engineered DNA construct into zygotes, followed by overnight cultivation in CO2 incubator and transfer of potentially modified embryos at the stage of two 4.0 blastomere to pseudopregnant foster females. A total of 91 living offspring were obtained and analyzed, with 18 pups carrying the target genome modification. The resulting transgenic animals were used to create a new line of mouse biomodels carrying the human HLA-A*02:01:01:01 gene.
{"title":"Embryological Aspects of Creating a New Humanized Transgenic Mouse Line with an Integrated Human Gene HLA A*02:01:01:01","authors":"E. S. Savchenko, N. S. Ogneva, N. Karkischenko","doi":"10.33647/2074-5982-18-4-10-23","DOIUrl":"https://doi.org/10.33647/2074-5982-18-4-10-23","url":null,"abstract":"The accumulation of scientific data in the field of pharmacogenetics requires the creation of adequate biomodels to reflect the immunogenetic characteristics of different population groups. We have obtained the ancestors of a new humanized transgenic mouse line carrying the human HLA-A*02:01:01:01 gene, which is characteristic of the Russian population. The new biomodels was created using the pronuclei microinjection method of a linearized fragment of genetically engineered DNA construct into zygotes, followed by overnight cultivation in CO2 incubator and transfer of potentially modified embryos at the stage of two 4.0 blastomere to pseudopregnant foster females. A total of 91 living offspring were obtained and analyzed, with 18 pups carrying the target genome modification. The resulting transgenic animals were used to create a new line of mouse biomodels carrying the human HLA-A*02:01:01:01 gene.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"278 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73239260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-18DOI: 10.33647/2074-5982-18-3-109-112
D. Ivkin, A. A. Karpov
An entire cycle of preclinical studies of 4-[(3-ethoxy-3-oxopropanoyl)amino]benzoic acid (etmaben) exhibiting cardiotropic action was carried out. Its potential action mechanism and pharmacological effects, general toxic effects in acute and chronic experiments, specific types of toxicity (allergenicity, immunotoxicity, reproductive toxicity, mutagenicity), and pharmacokinetics were studied. The drug was found to have a pronounced cardiotropic effect and a positive safety profile, which justifies the launch of phase I clinical trials.
{"title":"Experimental Evaluation of the Effectiveness and Safety of a New Propandic Acid Derivative Exhibiting Cardiotropic Action","authors":"D. Ivkin, A. A. Karpov","doi":"10.33647/2074-5982-18-3-109-112","DOIUrl":"https://doi.org/10.33647/2074-5982-18-3-109-112","url":null,"abstract":"An entire cycle of preclinical studies of 4-[(3-ethoxy-3-oxopropanoyl)amino]benzoic acid (etmaben) exhibiting cardiotropic action was carried out. Its potential action mechanism and pharmacological effects, general toxic effects in acute and chronic experiments, specific types of toxicity (allergenicity, immunotoxicity, reproductive toxicity, mutagenicity), and pharmacokinetics were studied. The drug was found to have a pronounced cardiotropic effect and a positive safety profile, which justifies the launch of phase I clinical trials.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82079619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-18DOI: 10.33647/2074-5982-18-3-99-103
Y. S. Alekseeva, Yu. N. Meshcheryakova, Ya. V. Shmakova, V. Bolotova
The effect of Eleutherococcus senticosus, Rhaponticum carthamoides and Schisandra chinensis extracts in doses of 10, 25, 50 and 75 mg/kg were studied. The actoprotective activity the studied adaptogens was assessed on outbred female mice in a forced swim test with a load of 10% of the animal's body weight. Dry extracts of Eleutherococcus senticosus (50 mg/kg), Rhaponticum carthamoides (10 mg/kg) and Schisandra chinensis (25 mg/kg) were found to exhibit the most pronounced actoprotective activity.
{"title":"Evaluation of the Effect of Phyto-Adaptogens on the Performance Capability of Outbred Female Mice","authors":"Y. S. Alekseeva, Yu. N. Meshcheryakova, Ya. V. Shmakova, V. Bolotova","doi":"10.33647/2074-5982-18-3-99-103","DOIUrl":"https://doi.org/10.33647/2074-5982-18-3-99-103","url":null,"abstract":"The effect of Eleutherococcus senticosus, Rhaponticum carthamoides and Schisandra chinensis extracts in doses of 10, 25, 50 and 75 mg/kg were studied. The actoprotective activity the studied adaptogens was assessed on outbred female mice in a forced swim test with a load of 10% of the animal's body weight. Dry extracts of Eleutherococcus senticosus (50 mg/kg), Rhaponticum carthamoides (10 mg/kg) and Schisandra chinensis (25 mg/kg) were found to exhibit the most pronounced actoprotective activity.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75924787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-18DOI: 10.33647/2074-5982-18-3-113-117
M. Melnikova, V. Kashuro, E. G. Batotsyrenova, L. G. Kubarskaya, E. A. Zolotoverkhaia, T. A. Kolbasova, O. Vakunenkova, A. Gladchuk
An experimental study of antioxidant parameters in rats after exposure to carbendazim was carried out to study the effect of their pharmacological correction by an intragastric administration of Saccharina latissima extract at a dose of 250 mg/kg for 14 days. The extract under study was found to reduce the activity of lipid peroxidation and promote the neutralization of hydrogen peroxide.
{"title":"Effect of Saccharina latissima Extract on the Antioxidant System Parameters of Rat Blood After Exposure to Carbendazim","authors":"M. Melnikova, V. Kashuro, E. G. Batotsyrenova, L. G. Kubarskaya, E. A. Zolotoverkhaia, T. A. Kolbasova, O. Vakunenkova, A. Gladchuk","doi":"10.33647/2074-5982-18-3-113-117","DOIUrl":"https://doi.org/10.33647/2074-5982-18-3-113-117","url":null,"abstract":"An experimental study of antioxidant parameters in rats after exposure to carbendazim was carried out to study the effect of their pharmacological correction by an intragastric administration of Saccharina latissima extract at a dose of 250 mg/kg for 14 days. The extract under study was found to reduce the activity of lipid peroxidation and promote the neutralization of hydrogen peroxide.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76375442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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