The workability of high fluidity concrete (HFC) also depends on its segregation resistance besides fluidity, and gap-passing ability, etc. Currently, there is a lack of easy, quantitative method for evaluating segregation resistance. Efficient assessment is crucial for construction applications of HFC. This paper aims to propose a simple test method for the segregation resistance of HFC on basis of the J-ring test that has been generally used for evaluating the fluidity and passing ability of HFC. Experiment and numerical simulation of J-ring test were conducted for HFCs with different fluidity and segregation resistance. The fresh concretes were treated as two-phase granular fluids of matrix mortar and coarse aggregate in simulation by a newly developed particle meshless method, called DPMP-MPS. The flow and segregation behaviors of the HFCs during J-ring test under different lifting speeds of slump cone were investigated. The numerical results demonstrate a close correlation between the final flow value to slump value ratio (SF-J/SL-J ratio) and the segregation resistances of HFCs. Consequently, the J-ring test can assess the segregation resistance based on the SF-J/SL-J ratio. Notably, it emphasizes that, for precise evaluation of HFC workability using the J-ring test, the lifting speed of the slump cone should fall within the range of 10 to 15 cm/s.