Pub Date : 2017-05-28DOI: 10.17265/2161-6256/2017.05.006
Johanis Ly, O. Sjofjan, I. H. Djunaidi, S. Suyadi
The study aimed at evaluating the effect of supplementing Saccharomyces cerevisiae into low quality local-based feeds on performance and nutrient digestibility of late starter to grower stage local pigs. There were total 16 late starter local pigs fed with four treatment feeds based on block design with four pigs in each treatment. The four treatment feeds offered consisted of: commercial starter feeds Charoen Pokphand 552 (T0), basal feeds + yeast 2% of daily feeds requirement (T1), basal feeds + yeast 4% of daily feeds requirement (T2) and basal feeds + yeast 6% of daily feeds requirement (T3). Feed intake, daily weight gain, feed conversion efficiency, protein and crude fiber digestibility were evaluated in the study. The findings showed that supplementing S. cerevisiae into low quality local-based diet improved 0.9%-2.7% feeds’ crude protein, 10%-19% feeds intake, 1%-6% daily weight gain, 3%-4% crude protein digestibility and 4%-5% crude fiber digestibility, but reduced feeds conversion efficiency by 0.3-0.4. The conclusion drawn is that supplementing S. cerevisiae (yeast) up to 6% improved performances of starter local pigs fed low quality feeds and performed the similar result with feeding commercial starter feeds Charoen Pokphand 552. Further research by widening the range and increasing the level of yeast supplementation could be done.
{"title":"Effect of Supplementing Saccharomyces cerevisiae into Low Quality Local-Based Feeds on Performance and Nutrient Digestibility of Late Starter Local Pigs","authors":"Johanis Ly, O. Sjofjan, I. H. Djunaidi, S. Suyadi","doi":"10.17265/2161-6256/2017.05.006","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.05.006","url":null,"abstract":"The study aimed at evaluating the effect of supplementing Saccharomyces cerevisiae into low quality local-based feeds on performance and nutrient digestibility of late starter to grower stage local pigs. There were total 16 late starter local pigs fed with four treatment feeds based on block design with four pigs in each treatment. The four treatment feeds offered consisted of: commercial starter feeds Charoen Pokphand 552 (T0), basal feeds + yeast 2% of daily feeds requirement (T1), basal feeds + yeast 4% of daily feeds requirement (T2) and basal feeds + yeast 6% of daily feeds requirement (T3). Feed intake, daily weight gain, feed conversion efficiency, protein and crude fiber digestibility were evaluated in the study. The findings showed that supplementing S. cerevisiae into low quality local-based diet improved 0.9%-2.7% feeds’ crude protein, 10%-19% feeds intake, 1%-6% daily weight gain, 3%-4% crude protein digestibility and 4%-5% crude fiber digestibility, but reduced feeds conversion efficiency by 0.3-0.4. The conclusion drawn is that supplementing S. cerevisiae (yeast) up to 6% improved performances of starter local pigs fed low quality feeds and performed the similar result with feeding commercial starter feeds Charoen Pokphand 552. Further research by widening the range and increasing the level of yeast supplementation could be done.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74028482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-05-28DOI: 10.17265/2161-6256/2017.05.008
Malvina María Prieto Laport, Pablo Ernesto Bobadilla Robledo, S. M. H. Cánen
Training and dissemination of good handling practices are key points in reducing animal abuse. Three aspects are considered in the development of education in animal welfare (AW): (1) introduction of AW in curricular education, nevertheless it has not been applied in the Facultad de Veterinaria, Universidad de la República de Uruguay, up to now; (2) promotion of permanent education for graduates; (3) development of plans for dissemination, follow up, assessment and continuous improvement on AW affairs. Aiming to obtain information about the knowledge of veterinarians on AW during the practice of their professional duties, in September 2016, an on-line questionnaire was sent to the veterinarians who were in the Educación Continua database. The questionnaire had four sections: a general gathering information and three additional sections, one for each veterinarian professional field (large animal’s health and production, small animal’s health and food industry). From 1,804 questionnaires sent, 388 answers were obtained. There were 69% attended at least one AW course, and 89.9% were willing to attend another AW course. And 63.5% considered the information received to be very useful for their daily activities, 95% considered that AW should be included in the veterinary studies curriculum, while 53.3% declared not having knowledge about the regional animal welfare strategy (RAWS). The majority of the veterinarians considered the acquired knowledge very useful and are eager to obtain more information. It is necessary to keep working to include AW in the veterinary studies curriculum as well as continuing with the diffusion of good practices and AW principles, based on the national and international norms.
{"title":"Animal Welfare in the Uruguayan Veterinary Profession Field","authors":"Malvina María Prieto Laport, Pablo Ernesto Bobadilla Robledo, S. M. H. Cánen","doi":"10.17265/2161-6256/2017.05.008","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.05.008","url":null,"abstract":"Training and dissemination of good handling practices are key points in reducing animal abuse. Three aspects are considered in the development of education in animal welfare (AW): (1) introduction of AW in curricular education, nevertheless it has not been applied in the Facultad de Veterinaria, Universidad de la República de Uruguay, up to now; (2) promotion of permanent education for graduates; (3) development of plans for dissemination, follow up, assessment and continuous improvement on AW affairs. Aiming to obtain information about the knowledge of veterinarians on AW during the practice of their professional duties, in September 2016, an on-line questionnaire was sent to the veterinarians who were in the Educación Continua database. The questionnaire had four sections: a general gathering information and three additional sections, one for each veterinarian professional field (large animal’s health and production, small animal’s health and food industry). From 1,804 questionnaires sent, 388 answers were obtained. There were 69% attended at least one AW course, and 89.9% were willing to attend another AW course. And 63.5% considered the information received to be very useful for their daily activities, 95% considered that AW should be included in the veterinary studies curriculum, while 53.3% declared not having knowledge about the regional animal welfare strategy (RAWS). The majority of the veterinarians considered the acquired knowledge very useful and are eager to obtain more information. It is necessary to keep working to include AW in the veterinary studies curriculum as well as continuing with the diffusion of good practices and AW principles, based on the national and international norms.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85492825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-05-28DOI: 10.17265/2161-6256/2017.05.003
Meirman Galiolla, Kenenbayev Serik, Yerzhanova Sakysh, A. Serik, T. Saltanat
The collection of alfalfa in the context of ecology-geographical groups was studied, consisting of 1,078 samples, including 985 samples of the cultivated species—M. sativa L. and M. varia Mart., and 93 samples for other wild species. Highly productive samples are allocated in the three-year cycle of using the grass stand in the haymaking regime. Individual genotypes of isolated samples are used for selection of inbred lines required for the formation of synthetic varieties. Inbred lines were created by forced self-pollination on genotypes with signs of self-fertility. Methodical experiments established the efficiency of bringing inbred lines to generations J2 and J3. In this case, the overall combinational ability reaches a potential value, and further incubation outside of J2 and J3 does not significantly affect its value. With the selection of the best inbred lines in J2 and J3, the lucerne varieties: Kokorai, Osimtal and Kokbalausa, which are protected by patents and author’s certificates, have been created in accordance with the general combination ability and their inclusion in the structure of synthetic varieties.
{"title":"Results of Selection Studies of Alfalfa Based on Inbred Lines","authors":"Meirman Galiolla, Kenenbayev Serik, Yerzhanova Sakysh, A. Serik, T. Saltanat","doi":"10.17265/2161-6256/2017.05.003","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.05.003","url":null,"abstract":"The collection of alfalfa in the context of ecology-geographical groups was studied, consisting of 1,078 samples, including 985 samples of the cultivated species—M. sativa L. and M. varia Mart., and 93 samples for other wild species. Highly productive samples are allocated in the three-year cycle of using the grass stand in the haymaking regime. Individual genotypes of isolated samples are used for selection of inbred lines required for the formation of synthetic varieties. Inbred lines were created by forced self-pollination on genotypes with signs of self-fertility. Methodical experiments established the efficiency of bringing inbred lines to generations J2 and J3. In this case, the overall combinational ability reaches a potential value, and further incubation outside of J2 and J3 does not significantly affect its value. With the selection of the best inbred lines in J2 and J3, the lucerne varieties: Kokorai, Osimtal and Kokbalausa, which are protected by patents and author’s certificates, have been created in accordance with the general combination ability and their inclusion in the structure of synthetic varieties.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76249440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, mitochondrial 16S rRNA sequences of snow leopard, gray wolf, domestic horse and Bactrian camel inhabited or domesticated in Mongolian territory were obtained by using polymerase chain reaction (PCR) based on universal primers for 16S rRNA (F-5'-AACGAGCCTGGTGATA-3' and R-5'-CTCCGGTCTGAACTCAGATCACGTA-3’). The 16S rRNA sequence was 1,048 bp to 1,086 bp in length, and each sequence was compared to other related species (Felidae, Camelidae, Equidae and Canidae) by using NCBI Basic Local Alignment Search Tool (BLAST). Results showed that sequences were highly similar to sequences in GenBank database (93%-99%). Then phylogenetic analysis was performed based on about 1,100 bp sequence of 16S rRNA for Panthera uncia, Canis lupus, Equus caballus, Camelus bactrianus and other related species. The result revealed that P. uncia and P. leo were sister species, C. bactrianus and C. ferus were more closely related species, and wolf and dog were the almost similar species. This finding could be important for designing species specific primers for PCR based analysis of animal species identification and forensic veterinary medicine.
{"title":"16S rRNA Gene Sequence Analysis of Snow Leopard, Gray Wolf, Horse and Bactrian Camel in Mongolia","authors":"Munkhtuul Tsogtgerel, Munkhtogtokh Baljijjnyam, Nansalmaa Suren, Lkhagvasuren Sodnom","doi":"10.17265/2161-6256/2017.05.007","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.05.007","url":null,"abstract":"In this study, mitochondrial 16S rRNA sequences of snow leopard, gray wolf, domestic horse and Bactrian camel inhabited or domesticated in Mongolian territory were obtained by using polymerase chain reaction (PCR) based on universal primers for 16S rRNA (F-5'-AACGAGCCTGGTGATA-3' and R-5'-CTCCGGTCTGAACTCAGATCACGTA-3’). The 16S rRNA sequence was 1,048 bp to 1,086 bp in length, and each sequence was compared to other related species (Felidae, Camelidae, Equidae and Canidae) by using NCBI Basic Local Alignment Search Tool (BLAST). Results showed that sequences were highly similar to sequences in GenBank database (93%-99%). Then phylogenetic analysis was performed based on about 1,100 bp sequence of 16S rRNA for Panthera uncia, Canis lupus, Equus caballus, Camelus bactrianus and other related species. The result revealed that P. uncia and P. leo were sister species, C. bactrianus and C. ferus were more closely related species, and wolf and dog were the almost similar species. This finding could be important for designing species specific primers for PCR based analysis of animal species identification and forensic veterinary medicine.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80123051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-28DOI: 10.17265/2161-6256/2017.04.001
René Tato Loua, M. Beavogui, H. Bencherif, A. Barry, Z. Bamba, C. Amory-Mazaudier
N'zerekore is a geographical region of Guinea, which houses a weather observatory located at longitude 8.8°, latitude 7.7° and altitude 475 m. This region is of major socioeconomic interest for the country. The climatological study of this region shows the variability of each parameter. The objective of this study was to analyze meteorological parameters trends and to assess the space-time evolution of some agro-climatic risks related to the main trends observed in the meteorological parameters regime of this region. In this study, programming tools were used for processing and analyzing meteorological parameters data, including temperatures, rains, wind, evaporation and storms measured in this observatory from 1931 to 2014. The interannual, annual and daily variations of these parameters were obtained, as well as temperature, precipitation anomalies and agroclimatic indexes trends. The analyzing of these variations explained that September is the rainiest months, and the year 1932, 1957 and 1970 are normal, rainy and dry year, respectively. The evaporation increased since 1971 from January to March and November to December. A positive temperature anomaly was observed since 1973 with the maximum 26-33 °C and the minimum 16-21 °C. A dominant westerly wind with a speed of 2.6 m/s was determined. Agro-climatic parameters in N'zerekore have high variability. From 1931 to 2014, three major periods can be distinguished: a wet period from 1931 to 1977, a dry period from 1978 to 1994 and rainfall variability from 1995 to 2014. The trend of these parameters explains the impact of climate change in this part of the world. This is exacerbated by human activity (deforestation), thus mitigation measures are necessary. It would be useful to extend this study throughout the country.
{"title":"Climatology of Guinea: Study of Climate Variability in N’zerekore","authors":"René Tato Loua, M. Beavogui, H. Bencherif, A. Barry, Z. Bamba, C. Amory-Mazaudier","doi":"10.17265/2161-6256/2017.04.001","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.04.001","url":null,"abstract":"N'zerekore is a geographical region of Guinea, which houses a weather observatory located at longitude 8.8°, latitude 7.7° and altitude 475 m. This region is of major socioeconomic interest for the country. The climatological study of this region shows the variability of each parameter. The objective of this study was to analyze meteorological parameters trends and to assess the space-time evolution of some agro-climatic risks related to the main trends observed in the meteorological parameters regime of this region. In this study, programming tools were used for processing and analyzing meteorological parameters data, including temperatures, rains, wind, evaporation and storms measured in this observatory from 1931 to 2014. The interannual, annual and daily variations of these parameters were obtained, as well as temperature, precipitation anomalies and agroclimatic indexes trends. The analyzing of these variations explained that September is the rainiest months, and the year 1932, 1957 and 1970 are normal, rainy and dry year, respectively. The evaporation increased since 1971 from January to March and November to December. A positive temperature anomaly was observed since 1973 with the maximum 26-33 °C and the minimum 16-21 °C. A dominant westerly wind with a speed of 2.6 m/s was determined. Agro-climatic parameters in N'zerekore have high variability. From 1931 to 2014, three major periods can be distinguished: a wet period from 1931 to 1977, a dry period from 1978 to 1994 and rainfall variability from 1995 to 2014. The trend of these parameters explains the impact of climate change in this part of the world. This is exacerbated by human activity (deforestation), thus mitigation measures are necessary. It would be useful to extend this study throughout the country.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83561024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-28DOI: 10.17265/2161-6256/2017.04.003
Awatef M. Badr-Elden, I. Ibrahim, H. Emara, M. Gabr, A. Nower, Tamer Mahfouz Abd Elaziem
Date palm, like all other crops, is very sensitive to the injury by many insect pests, which may lead to the death of the affected plant and causes decrease in yield. In the present study, an efficient Agrobacterium for genetic transformation was successfully achieved for well known date palm (Phoenix dactylifera L.) cv. Medjool and Khalas using callus as explant. Embryogenic callus were recorded 100% mortality when cultured on MS medium containing 100 mg/L kanamycin with different cultivars, thus it was chosen for the selection of transformed explants. Embryogenic callus of Medjool and Khalas were incubated with Agrobacterium tumefaciens strain LBA 4404 for 0.5, 1, 2, 4 and 24 h on LB medium. After the incubation periods, embryogenic callus was transferred to MS medium with 0.1 mg/L NAA, 0.05 mg/L BA, 250 mg/L carbenicillin and 100 mg/L kanamycin for detection of transgenic embryogenic callus. Polymerase chain reaction (PCR) was used for the rapid screening of Cry3Aa gene. For screening, total genomic DNA was isolated from transformants. Using primer specific to Cry3Aa gene (forward and reverse), a PCR product with a size of about 2,000 bp was amplified when all nucleic acid from the transformants were utilized as templates. PCR analysis confirmed the appearance of the transgene of 2,000 bp in one individual plantlet. Presence and integration of foreign Cry3Aa gene in regenerated kanamycin resistant embryogenic callus was also confirmed by Southern blot hybridization. It was found that one transgenic embryogenic callus for both Medjool and Khalas showed a single copy of gene integration. These results signify the successful transfer of Cry3Aa gene into date palm plant.
{"title":"Transgenic Date Palm Containing Endotoxin Cry3Aa Gene","authors":"Awatef M. Badr-Elden, I. Ibrahim, H. Emara, M. Gabr, A. Nower, Tamer Mahfouz Abd Elaziem","doi":"10.17265/2161-6256/2017.04.003","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.04.003","url":null,"abstract":"Date palm, like all other crops, is very sensitive to the injury by many insect pests, which may lead to the death of the affected plant and causes decrease in yield. In the present study, an efficient Agrobacterium for genetic transformation was successfully achieved for well known date palm (Phoenix dactylifera L.) cv. Medjool and Khalas using callus as explant. Embryogenic callus were recorded 100% mortality when cultured on MS medium containing 100 mg/L kanamycin with different cultivars, thus it was chosen for the selection of transformed explants. Embryogenic callus of Medjool and Khalas were incubated with Agrobacterium tumefaciens strain LBA 4404 for 0.5, 1, 2, 4 and 24 h on LB medium. After the incubation periods, embryogenic callus was transferred to MS medium with 0.1 mg/L NAA, 0.05 mg/L BA, 250 mg/L carbenicillin and 100 mg/L kanamycin for detection of transgenic embryogenic callus. Polymerase chain reaction (PCR) was used for the rapid screening of Cry3Aa gene. For screening, total genomic DNA was isolated from transformants. Using primer specific to Cry3Aa gene (forward and reverse), a PCR product with a size of about 2,000 bp was amplified when all nucleic acid from the transformants were utilized as templates. PCR analysis confirmed the appearance of the transgene of 2,000 bp in one individual plantlet. Presence and integration of foreign Cry3Aa gene in regenerated kanamycin resistant embryogenic callus was also confirmed by Southern blot hybridization. It was found that one transgenic embryogenic callus for both Medjool and Khalas showed a single copy of gene integration. These results signify the successful transfer of Cry3Aa gene into date palm plant.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87838094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-28DOI: 10.17265/2161-6256/2017.04.005
B. K. Tekelioglu, M. Celik, Aygül Küçükgülmez
The aim of this case report was to demonstrate the effectivity of chitosan as topical agent on the treatment of severely infected canine extremity wound. The dog was hit by a bus and presented severe friction wounds accompanying with bilateral hip and extremity fractures. Local and generalized infections were observed on the post operational period. The dog was unresponsive to the standard therapeutic protocols and health status was getting worsened. The regenerative sutures did not hold the tissues because of the infection and the tissue eruption. Chitosan samples were extracted from shrimp shell wastes. The milled form of chitosan was topically used to treat the open and heavily mix infected wound areas located at skin and rear extremities accompanying with fever and Canine coronavirus diarrhea. It was decided to use the chitosan as a bio barrier and anti-bio affect over the damaged and erupted tissues. Macroscopic findings indicated chitosan promoted the natural blood clotting and absorbed the inflammation fluid. Microscopic findings indicated the infiltration of polymorph nuclear leukocytes (PMN) accelerated with the topical use of chitosan in the early phase of wound healing. The wound healing was observed daily. This veterinary practice wound treatment result indicated that chitosan is effective to medicate topically the canine open and mix infected wounds with bacteria and viruses and promotes the granulomatosis in four weeks period.
{"title":"Canine Extremity Wound Treatment with Chitosan Extracted from Shrimp Shells: A Case Report","authors":"B. K. Tekelioglu, M. Celik, Aygül Küçükgülmez","doi":"10.17265/2161-6256/2017.04.005","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.04.005","url":null,"abstract":"The aim of this case report was to demonstrate the effectivity of chitosan as topical agent on the treatment of severely infected canine extremity wound. The dog was hit by a bus and presented severe friction wounds accompanying with bilateral hip and extremity fractures. Local and generalized infections were observed on the post operational period. The dog was unresponsive to the standard therapeutic protocols and health status was getting worsened. The regenerative sutures did not hold the tissues because of the infection and the tissue eruption. Chitosan samples were extracted from shrimp shell wastes. The milled form of chitosan was topically used to treat the open and heavily mix infected wound areas located at skin and rear extremities accompanying with fever and Canine coronavirus diarrhea. It was decided to use the chitosan as a bio barrier and anti-bio affect over the damaged and erupted tissues. Macroscopic findings indicated chitosan promoted the natural blood clotting and absorbed the inflammation fluid. Microscopic findings indicated the infiltration of polymorph nuclear leukocytes (PMN) accelerated with the topical use of chitosan in the early phase of wound healing. The wound healing was observed daily. This veterinary practice wound treatment result indicated that chitosan is effective to medicate topically the canine open and mix infected wounds with bacteria and viruses and promotes the granulomatosis in four weeks period.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90309151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-28DOI: 10.17265/2161-6256/2017.04.006
W. Oh, V. Nguyen, H. Moon, Jeehoon Lee, H. Kim, Seongjun Park, H. Chung, B. Park
Infectious agents causing aborted fetus problems in domestic pigs were investigated in this study. More than 10 different infectious agents were known to cause abortion in swine and the major eight viruses among them were inspected. One hundred twelve samples of aborted fetuses from nine provinces in South Korea were collected during April to November, 2013 in this study for the diagnosis of infectious agents causing abortions in pigs. Eight major infection viruses were examined in this study mainly using various diagnostic kits and reverse transcriptase polymerase chain reaction (RT-PCR). Positive rate of the detection differed from each viruses. In this study, the main focus was the porcine reproductive and respiratory syndrome virus (PRRSV), which took the second large portion in the positive rate of detection, and then its ORF5 gene was compared with modified live virus (MLV) vaccine strain to figure out the influence of vaccine on disease. Between four positive samples’ sequence, two of them were 99.9%-100% similar to MLV vaccine strain and two other samples were 88.6%-92.7% similar. Similarity rate of the sequences between the vaccine and virus from aborted fetuses are very crucial, because it implies that abortion in swine can be made due to the usage of vaccine not only by the infection of field virus, and if MLV vaccine actually do have an impact on the infection, usage of the vaccine should be reconsidered.
{"title":"Investigation on Infectious Agents of Aborted Pig Fetuses and Its Correlation with PRRSV MLV Vaccine","authors":"W. Oh, V. Nguyen, H. Moon, Jeehoon Lee, H. Kim, Seongjun Park, H. Chung, B. Park","doi":"10.17265/2161-6256/2017.04.006","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.04.006","url":null,"abstract":"Infectious agents causing aborted fetus problems in domestic pigs were investigated in this study. More than 10 different infectious agents were known to cause abortion in swine and the major eight viruses among them were inspected. One hundred twelve samples of aborted fetuses from nine provinces in South Korea were collected during April to November, 2013 in this study for the diagnosis of infectious agents causing abortions in pigs. Eight major infection viruses were examined in this study mainly using various diagnostic kits and reverse transcriptase polymerase chain reaction (RT-PCR). Positive rate of the detection differed from each viruses. In this study, the main focus was the porcine reproductive and respiratory syndrome virus (PRRSV), which took the second large portion in the positive rate of detection, and then its ORF5 gene was compared with modified live virus (MLV) vaccine strain to figure out the influence of vaccine on disease. Between four positive samples’ sequence, two of them were 99.9%-100% similar to MLV vaccine strain and two other samples were 88.6%-92.7% similar. Similarity rate of the sequences between the vaccine and virus from aborted fetuses are very crucial, because it implies that abortion in swine can be made due to the usage of vaccine not only by the infection of field virus, and if MLV vaccine actually do have an impact on the infection, usage of the vaccine should be reconsidered.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73335910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-04-28DOI: 10.17265/2161-6256/2017.04.002
F. Ishola, S. Aboaba, M. Choudhary, O. Ekundayo
The chemical compositions of the essential oils obtained from six tree parts of Chrysophyllum albidum (Sapotaceae) were extracted by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). A total of 65, 33, 45, 21, 25 and 18 compounds, representing 79.49%, 100%, 90.81%, 98.43%, 96.62% and 98.37% of the total oil, were identified in the fruit bark, root bark, stem bark, seed bark, leaf and seed, respectively. The dominant compounds in the essential oils in six tree parts were m-xylene (66.7%; seed), p-xylene (21.4%; seed bark), α-farnesene (38.1%; leaf), hexadecanoic acid (14.7%; stem bark), m-xylene (53.1%; root bark) and hexadecanoic acid (12.7%; fruit bark). The essential oils were evaluated for their antibacterial, antioxidant and insecticidal activities using Alamar blue assay, DPPH radical scavenging activity and contact toxicity test, respectively. The oils displayed moderate antibacterial potentials to some tested organisms and low radical scavenging activity to DPPH. Rhyzopertha dominica was susceptible to C. albidum stem bark essential oil only.
{"title":"Chemical and Biological Assessments of the Essential Oils of Chrysophyllum albidum G. Don","authors":"F. Ishola, S. Aboaba, M. Choudhary, O. Ekundayo","doi":"10.17265/2161-6256/2017.04.002","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.04.002","url":null,"abstract":"The chemical compositions of the essential oils obtained from six tree parts of Chrysophyllum albidum (Sapotaceae) were extracted by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). A total of 65, 33, 45, 21, 25 and 18 compounds, representing 79.49%, 100%, 90.81%, 98.43%, 96.62% and 98.37% of the total oil, were identified in the fruit bark, root bark, stem bark, seed bark, leaf and seed, respectively. The dominant compounds in the essential oils in six tree parts were m-xylene (66.7%; seed), p-xylene (21.4%; seed bark), α-farnesene (38.1%; leaf), hexadecanoic acid (14.7%; stem bark), m-xylene (53.1%; root bark) and hexadecanoic acid (12.7%; fruit bark). The essential oils were evaluated for their antibacterial, antioxidant and insecticidal activities using Alamar blue assay, DPPH radical scavenging activity and contact toxicity test, respectively. The oils displayed moderate antibacterial potentials to some tested organisms and low radical scavenging activity to DPPH. Rhyzopertha dominica was susceptible to C. albidum stem bark essential oil only.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78347276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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