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Microbiochemical reactors for enzymatic reactions including cell-free mRNA translation 用于酶促反应的微生化反应器,包括无细胞mRNA翻译
Pub Date : 1900-01-01 DOI: 10.1163/156856300744687
T. Fujii, S. Shoji, T. Nojima, A. Yotsumoto, K. Hosokawa, I. Endo
As the first step toward the 'lab on a chip' or 'cell on a chip' concept, several microbiochemical reactors were fabricated using conventional MEMS (microelectromechanical systems) techniques: silicon anisotropic etching and glass-silicon anodic bonding. The microreactors have main reaction channels which are 14 mm long, 200-800 μm wide, and 20 μm deep. In these channels, enzymatic reactions - luciferin-luciferase reaction and cell-free mRNA translation - were demonstrated. Luminescence induced by the luciferin-luciferase reaction was observed in two ways, by photograph on a high-speed film and sensitive video imaging. In both cases, diffusion-based mixing in the microreactors was clearly visualized. In the cell-free protein synthesis experiment, the amount of polyphenylalanine, which was synthesized by translating polyuridylic acid template in the microreactors, was determined using radioisotope assay.
作为迈向“芯片上的实验室”或“芯片上的细胞”概念的第一步,使用传统的MEMS(微机电系统)技术制造了几个微生化反应器:硅各向异性蚀刻和玻璃硅阳极键合。微反应器主反应通道长14 mm,宽200 ~ 800 μm,深20 μm。在这些通道中,酶促反应-荧光素-荧光素酶反应和无细胞mRNA翻译-被证实。荧光素-荧光素酶反应诱导的发光以两种方式观察,一种是高速胶片照相,另一种是灵敏视频成像。在这两种情况下,微反应器中基于扩散的混合都清晰可见。在无细胞蛋白合成实验中,采用放射性同位素法测定多尿苷酸模板在微反应器中翻译合成的聚苯丙氨酸的量。
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引用次数: 5
Study on an injection quantity sensor. I: Evaluation of the integration procedure 一种注射量传感器的研究。1:对整合程序的评价
Pub Date : 1900-01-01 DOI: 10.1163/156856305323383892
Seidel, Schmid
For 'on board' diagnosis purposes of the injected fuel quantity, ceramic flow sensor chips with a diameter of 2.2 mm were integrated into finished Common Rail injection nozzles of the valve covered orifice (VCO) or mini-sac hole (MSH) class. The influence of this integration step on the hydraulic performance of the high-precision injector nozzles which led to an optimized process flow with a minimized disturbance of the fluidic properties was investigated. Electron-discharge machining and electron-beam welding technology proved to be the key technologies for this integration process, providing a reliable solution for injection pressures ranging up to 135 MPa (1350 bar). With a high-pressure hydraulic test bench the decrease in the injected fuel quantity as a function of welding parameters was determined for both nozzle classes. The geometrical distortion of the nozzle was mainly attributed to a radial bending of the nozzle tip and is dominated by the heat concentration during the welding process. In comparison, the integration of the ceramic sensor chip causes lower distortion values. By reducing the thermal energy input during welding a negligible influence of the complete integration process with respect to the hydraulic performance of original orifices is demonstrated at MSH injection nozzles.
为了对喷射燃油量进行“机载”诊断,将直径为2.2 mm的陶瓷流量传感器芯片集成到阀盖孔(VCO)或微囊孔(MSH)级的成品共轨喷射喷嘴中。研究了该集成步骤对高精度喷油器水力性能的影响,从而实现了流体性能扰动最小的优化工艺流程。事实证明,电火花加工和电子束焊接技术是这一集成过程的关键技术,为高达135 MPa (1350 bar)的注射压力提供了可靠的解决方案。利用高压液压试验台,确定了两种喷嘴类型的喷油量随焊接参数的变化规律。喷嘴的几何变形主要是由于喷嘴尖端的径向弯曲造成的,焊接过程中的热集中是主要原因。相比之下,陶瓷传感器芯片的集成导致更低的失真值。通过减少焊接过程中的热能输入,证明了在MSH注射喷嘴中,完整的集成过程对原始孔板水力性能的影响可以忽略不计。
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引用次数: 5
Real-time object tracking for the robot-based nanohandling in a scanning electron microscope 扫描电子显微镜下机器人纳米处理的实时目标跟踪
Pub Date : 1900-01-01 DOI: 10.1163/156856306777924644
S. Fatikow, T. Sievers
In this paper, current research work on an automated nanohandling station using mobile microrobots is presented. For the automated positioning of mobile microrobots a closed-loop control system is necessary, usually using data from pose sensors for the degrees of freedom (DOF) of the microrobot are needed. Mobile microrobots with piezo slip-stick actuation mostly do not have internal pose sensors to determine a global pose. This paper focuses on the continuous pose estimation (tracking) of mobile microrobots by external visual sensors. One possibility for fast pose estimation is the application of video cameras in combination with image processing algorithms as global sensors. However, for pose estimation with accuracy in the nanometer range high-resolution sensors are necessary. In consideration of resolution, image acquisition time and depth of focus a scanning electron microscope (SEM) is a powerful sensor for high-resolution pose estimation of a microrobot. On the other hand, the use of a SEM requires high demands on the image processing. High update rates of the pose data for the robot control require a short image acquisition time of the SEM images. As a result, the image noise increases as frame averaging or averaging of the detector signal is time consuming. This paper presents two approaches to tracking a micro-object in a SEM image stream. First, a cross-correlation algorithm is described, which enables pose estimation (x, y, ϕ) in extremely noised images in real-time. Afterwards object tracking with active contours is presented. This approach allows real-time tracking with more than 3 DOF by using shape spaces, instead of defining large model sets as it is necessary for correlation-based pattern matching.
本文介绍了移动微型机器人自动化纳米处理站的研究现状。为了实现移动微型机器人的自动定位,需要一个闭环控制系统,通常需要利用位姿传感器的数据来确定微型机器人的自由度。具有压电滑棒驱动的移动微型机器人大多没有内部姿态传感器来确定全局姿态。本文主要研究了基于外部视觉传感器的移动微型机器人连续姿态估计(跟踪)问题。快速姿态估计的一种可能性是将摄像机与图像处理算法结合起来作为全局传感器。然而,要在纳米范围内精确估计姿态,需要高分辨率传感器。从分辨率、图像采集时间和聚焦深度等方面考虑,扫描电子显微镜是实现微型机器人高分辨率姿态估计的有力传感器。另一方面,扫描电镜的使用对图像处理的要求很高。为了提高机器人姿态数据的更新速度,需要较短的扫描电镜图像采集时间。结果,由于对检测信号进行帧平均或平均耗时,图像噪声增大。本文提出了在扫描电镜图像流中跟踪微目标的两种方法。首先,描述了一种相互关联算法,该算法可以实时地在极噪图像中进行姿态估计(x, y, ϕ)。然后提出了基于活动轮廓的目标跟踪方法。这种方法允许使用形状空间进行超过3自由度的实时跟踪,而不是定义基于关联的模式匹配所必需的大型模型集。
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引用次数: 76
Micro-motion selective-actuation X Y Z flexure parallel mechanism: design and modeling 微动选择性驱动X Y Z挠性并联机构:设计与建模
Pub Date : 1900-01-01 DOI: 10.1163/156856305323383919
H. Pham, I. Chen, H. Yeh
This paper presents the design of a selective-actuation flexure parallel mechanism that can provide three de-coupled micro-motions along the X -, Y - and Z -axes. This mechanism can be used as an ultra-precision positioning system. The modeling of this flexure parallel mechanism is then established based on a pseudo-rigid-body model with consideration of deformation of the flexure member. The factor of deformation allows us to formulate the accurate kinematics analysis of the flexure mechanism. Via this modeling, dimension and free shape of the mechanism are determined based on the criteria of isotropic resolution transmission scale. An experiment was set up to verify the modeling and the design. The experiment shows the advantage of the proposed model versus the model currently used. The similarity of the resolutions obtained from the experiment and predicted by the optimal design shows the validity of the resolution evaluation and the optimal design.
本文设计了一种选择驱动柔性并联机构,该机构可以沿X、Y和Z轴进行三种解耦微运动。该机构可作为超精密定位系统。在考虑挠性构件变形的拟刚体模型的基础上,建立了该挠性并联机构的模型。变形因素使我们能够对挠性机构进行精确的运动学分析。通过该模型,根据各向同性分辨率传输尺度准则确定了机构的尺寸和自由形状。通过实验验证了模型和设计的正确性。实验结果表明,本文提出的模型相对于目前使用的模型具有一定的优越性。实验得到的分辨率与优化设计预测的分辨率相似,表明了分辨率评价和优化设计的有效性。
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引用次数: 19
Design of a flexible conveyer microrobot with electromagnetic field-based friction drive control for microfactory stations 基于电磁场摩擦驱动控制的微型工厂工位柔性输送机器人设计
Pub Date : 1900-01-01 DOI: 10.1163/156856300744669
Antoine Ferreira
This paper proposes and demonstrates the feasibility of a new generation of compliant piezoelectric microconveyers for microobjects based on the cooperation of arrayed direct-drive standing wave ultrasonic motors (microSWUMs). Their operating principles, which combine active frictional contact mechanisms with electromagnetic field-based friction drive control, are analyzed. A prototype of a planar-type ultrasonic motor incorporating 48 arrayed microSWUMs, whose overall dimensions are 47 × 29 mm2, has been realized through the use of a flexible active metallic sheet (stator) which acts as a mobile platform. An experimental characterization has been carried out in order to identify the main physical and technological limitations specific to millimeter scale standing wave motors. The corresponding results indicate that powerful force/speed characteristics on the millimeter scale can be expected, i.e. high output force density around 20 mN · mm-2, a carried load ratio reaching 1 kg · cm-2, a traveling distance without displacement restrictions, and a nanometer resolution of positioning.
本文提出并论证了基于阵列直驱驻波超声电机(microSWUMs)合作的新一代柔性微物体压电输送机的可行性。分析了主动摩擦接触机构与基于电磁场的摩擦驱动控制相结合的工作原理。利用柔性有源金属片(定子)作为移动平台,实现了一个整体尺寸为47 × 29 mm2、包含48个阵列微型swum的平面型超声电机原型。为了确定毫米尺度驻波电机的主要物理和技术限制,进行了实验表征。结果表明,在毫米尺度上具有强大的力/速度特性,即输出力密度约为20 mN·mm-2,承载载荷比达到1 kg·cm-2,移动距离不受位移限制,定位分辨率达到纳米级。
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引用次数: 9
Integrated micromachined components for biological analysis systems 用于生物分析系统的集成微机械组件
Pub Date : 1900-01-01 DOI: 10.1163/156856300744678
A. B. Frazier, I. Papautsky, B. Gale, K. Caldwell
In this paper, we provide an overview of miniaturized biochemical analysis system components and specifically discuss the micromachined metallic pipette arrays (MPA) and the micromachined electrical field-flow fractionation system (μ-EFFF). The MPA are arrays of hollow metallic pipettes extending from a silicon substrate. These arrays are used in macroscale spatial manipulation of samples in the pl to μl range. The μ-EFFF system is composed of a micromachined flow channel with integrated electrodes for application of static electric fields. The system is used for separation and analysis of biological molecules and colloids. The design, fabrication, and characterization of the MPA and the μ-EFFF are described. In addition, the integration of the components using micromechatronics technologies is discussed.
本文综述了小型化生化分析系统的组成,重点讨论了微机械金属移液阵列(MPA)和微机械电场流分馏系统(μ-EFFF)。MPA是从硅衬底延伸出来的中空金属移液器阵列。这些阵列用于在pl到μl范围内的样品的宏观尺度空间操作。μ-EFFF系统由微机械流道和集成电极组成,用于静电作用。该系统用于生物分子和胶体的分离和分析。介绍了MPA和μ-EFFF的设计、制作和表征。此外,还讨论了利用微机电一体化技术实现各部件的集成。
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引用次数: 1
Fascination of down scaling — Alice the sugar cube robot 缩小比例的魅力——方糖机器人爱丽丝
Pub Date : 1900-01-01 DOI: 10.1163/156856301760132097
G. Caprari, T. Estier, R. Siegwart
Design of mobile micro-robot (MMR) is still a challenge due to the restricted availability of basic components. However, the number of highly integrated microelectronic and micromechanical components is growing fast. Nevertheless, its integration to a micro-system requires a good knowledge of all the interactions between sensor, actuator, computation and energy source. Often compromises between performance and power consumption have to be found. This paper gives the basic considerations for building mobile micro-robots. The major scaling effects are presented and their impact on micro-system design is discussed. The mobile micro-robot Alice (Fig. 1), having the size of a sugar cube, is presented and discussed in the context of scaling laws. It has an autonomy of around 10 hours and is able to navigate based on simple behaviors like obstacle avoidance or wall following.
由于基本部件的可用性有限,移动微型机器人的设计仍然是一个挑战。然而,高度集成的微电子和微机械元件的数量正在快速增长。然而,将其集成到微系统中需要很好地了解传感器,执行器,计算和能源之间的所有相互作用。通常需要在性能和功耗之间找到折衷方案。本文给出了构建移动微型机器人的基本考虑。介绍了主要的尺度效应,并讨论了它们对微系统设计的影响。本文提出并讨论了大小为方糖大小的移动微型机器人Alice(图1)。它有大约10个小时的自主性,能够根据简单的行为进行导航,比如避开障碍物或跟着墙走。
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引用次数: 44
Networked human/robot cooperative environment for tele-assembly of MEMS devices 面向MEMS器件远程装配的网络化人机协作环境
Pub Date : 1900-01-01 DOI: 10.1163/156856306777924626
Yantao Shen, N. Xi, Bo Song, W. Li, C. Pomeroy
The objective of this paper is to develop a networked cooperative environment to achieve human/robot cooperation for reliable and dependable remote microassembly. At a microscale, surface adhesion forces, such as van der Waals, surface tension and electrostatic forces, become stronger than the downward gravitational force. For a reliable and dependable tele-microassembly, it is absolutely necessary to allow close monitoring of the magnitude and direction of those micro-forces interacting with microdevices during the assembly process. In this paper, based on integrating an in situ polyvinylidene fluoride piezoelectric micro-force sensing tool with a resolution in the range of μN, and using event-synchronization for the feedback of assembly video and micro-force, the developed networked human/robot cooperative platform can greatly advance applications in tele-microassembly. As a result, the reliable and dependable human/robot cooperative assembly operations can be achieved and extended to the single or multiple remote work-cells through a local area network or the Internet. This platform has been used successfully to perform a remote assembly of surface MEMS structures with the event-synchronized micro-force/visual feedback via the Internet between USA and Hong Kong.
本文的目标是开发一个网络化的协作环境,以实现可靠、可靠的远程微装配的人/机器人协作。在微观尺度下,表面附着力,如范德华力、表面张力和静电力,变得比向下的重力更强。为了实现可靠的远程微装配,在装配过程中对与微器件相互作用的微力的大小和方向进行密切监测是绝对必要的。本文在集成分辨率为μN的聚偏氟乙烯压电微力传感工具的基础上,利用事件同步对装配视频和微力进行反馈,开发了网络化的人/机器人协作平台,可大大推进远程微装配的应用。通过局域网或Internet,可以实现可靠、可靠的人/机器人协同装配操作,并将其扩展到单个或多个远程工作单元。该平台已成功地通过互联网在美国和香港之间进行了具有事件同步微力/视觉反馈的表面MEMS结构的远程组装。
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引用次数: 7
Design and control of a pneumatic microrobot for in-pipe inspection of nuclear pipes 核能管道内检测气动微型机器人的设计与控制
Pub Date : 1900-01-01 DOI: 10.1163/156856301753398172
M. Bétemps, C. Anthierens
Micro Electro Mechanical Systems (MEMS) are integrated in many current products and are not only the concern of military defence or medicine. Nowadays, many different types of microactuators exist which use different types of energy and perform a variety of movements. Several applications require small systems to inspect confined and hostile places. Vapour generators in nuclear plants comprise of 3000 to 5000 vertical pipes of 17 mm diameter. These pipes endure high mechanical constraints and have to be inspected to detect possible cracks. Our study is based on the design, modelling and implementation of a microrobot able to move upwards within and carry sensors into these pipes. It moves as an inchworm and is composed of 2 blocking modules that brace the robot on the pipe sides, and one stretching module that creates a step. This actuator is pneumatic and composed of metal bellows. By this original design, the microrobot has a good power-to-volume ratio and it can carry a load of 600 g. Its good positioning accuracy has been proved over a 90 mm course where the error of positioning is less than 60 μm.
微机电系统(MEMS)集成在当今许多产品中,不仅仅是军事防御或医学关注的问题。目前,存在许多不同类型的微致动器,它们使用不同类型的能量并执行各种运动。一些应用需要小型系统来检查密闭和恶劣的场所。核电站的蒸汽发生器由3000至5000根直径为17毫米的垂直管道组成。这些管道承受着很高的机械约束,必须进行检查以发现可能的裂缝。我们的研究是基于一个微型机器人的设计、建模和实现,它能够在这些管道中向上移动,并将传感器携带到这些管道中。它像尺蠖一样移动,由2个阻塞模块组成,用于支撑机器人在管道两侧,一个拉伸模块用于创建台阶。这种执行器是气动的,由金属波纹管组成。通过这种原始设计,微型机器人具有良好的功率体积比,可以承载600克的负载。在90 mm的航线上,定位误差小于60 μm,具有良好的定位精度。
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引用次数: 6
Automatic dextrous microhandling based on a 6-DOF microgripper 基于六自由度微夹持器的自动灵巧微处理
Pub Date : 1900-01-01 DOI: 10.1163/156856306777924662
Quan Zhou, P. Korhonen, J. Laitinen, S. Sjövall
The demand for automatic quality inspection of individual micro-components has increased strongly in the micro-optoelectronics industry. In many cases, quality inspection of those micro-components needs dexterous microhandling. However, automatic dexterous handling of microcomponents is a very challenging issue which is barely explored. This paper presents a high-DOF (degrees of freedom) automatic dexterous handling and inspection system for micro-optoelectronic components using a novel 6-DOF piezoelectric microgripper. The control system includes three hierarchical layers: an actuator control layer, a motion planning layer and a mission control layer. Machine vision is applied in automatic manipulation. The performance of the system is demonstrated in a vision-based automatic inspection task for 300 × 300 × 100- μ m-sized optoelectronics components and reached a cycle time of 7.1 ± 0.3 s.
在微光电子工业中,对单个微元件的自动质量检测的需求日益增长。在许多情况下,这些微型部件的质量检测需要灵巧的微处理。然而,微型部件的自动灵巧处理是一个非常具有挑战性的问题,很少有人探索。介绍了一种新型的六自由度压电微夹持器,用于微光电元件的高自由度自动灵巧搬运与检测系统。控制系统包括三个层次:执行器控制层、运动规划层和任务控制层。机器视觉在自动操作中的应用。在300 × 300 × 100 μ m尺寸的光电元件的视觉自动检测任务中,系统的性能得到了验证,周期时间为7.1±0.3 s。
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引用次数: 39
期刊
Journal of Micromechatronics
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