Pub Date : 2022-08-26DOI: 10.1177/08839115221119210
Asal Ebrahimzadeh, Elnaz Khanalizadeh, Shahla Khodabakhshaghdam, D. Kazemi, Ali Baradar Khoshfetrat
Injectable in situ-forming hydrogels appears to be a promising approach for tissue engineering applications. In this study, the effect of phenol moiety (Ph) addition to gelatin in enzymatically-gellable modified pectin hydrogel (Pec-Ph) was studied. Addition of gelatin-Ph to Pec-Ph (Pec-Ph/Gel-Ph) altered the physical properties of Pec-Ph-based hydrogels as compared to unmodified gelatin (Pec-Ph/Gel) addition. Swelling ratio and degradation rates of the Pec-Ph/Gel-Ph hydrogel decreased 35% and 50%, respectively, and the elasticity of Pec-Ph/Gel-Ph hydrogel was higher than the Pec-Ph/Gel hydrogels. Scanning electron microscopy images showed that the existence of phenolic groups in gelatin decreased the pore size of Pec-Ph/Gel-Ph hydrogels. Culture of chondrocyte cells in the Pec-Ph/Gel-Ph hydrogels showed more metabolic activity (4×) during a 14-day culture period. Hydrogels subcutaneously implanted in rats could also be identified readily without complete absorption and signs of toxicity or any untoward reactions after 1 month. The work showed the potential of Pec-Ph/Gel-Ph hydrogels as a promising in situ injectable hydrogel for soft tissue engineering applications.
{"title":"Influence of gelatin modification on enzymatically-gellable pectin-gelatin hydrogel properties for soft tissue engineering applications","authors":"Asal Ebrahimzadeh, Elnaz Khanalizadeh, Shahla Khodabakhshaghdam, D. Kazemi, Ali Baradar Khoshfetrat","doi":"10.1177/08839115221119210","DOIUrl":"https://doi.org/10.1177/08839115221119210","url":null,"abstract":"Injectable in situ-forming hydrogels appears to be a promising approach for tissue engineering applications. In this study, the effect of phenol moiety (Ph) addition to gelatin in enzymatically-gellable modified pectin hydrogel (Pec-Ph) was studied. Addition of gelatin-Ph to Pec-Ph (Pec-Ph/Gel-Ph) altered the physical properties of Pec-Ph-based hydrogels as compared to unmodified gelatin (Pec-Ph/Gel) addition. Swelling ratio and degradation rates of the Pec-Ph/Gel-Ph hydrogel decreased 35% and 50%, respectively, and the elasticity of Pec-Ph/Gel-Ph hydrogel was higher than the Pec-Ph/Gel hydrogels. Scanning electron microscopy images showed that the existence of phenolic groups in gelatin decreased the pore size of Pec-Ph/Gel-Ph hydrogels. Culture of chondrocyte cells in the Pec-Ph/Gel-Ph hydrogels showed more metabolic activity (4×) during a 14-day culture period. Hydrogels subcutaneously implanted in rats could also be identified readily without complete absorption and signs of toxicity or any untoward reactions after 1 month. The work showed the potential of Pec-Ph/Gel-Ph hydrogels as a promising in situ injectable hydrogel for soft tissue engineering applications.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"13 1","pages":"381 - 391"},"PeriodicalIF":1.7,"publicationDate":"2022-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85972169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-22DOI: 10.1177/08839115221119211
Yaocheng Wang, Chengxiong Lin
Biological 3D printing is a reliable technology for 3D printing bone repair scaffolds with simple operation, high efficiency, and relatively low cost. Gelatin methacryloyl (GelMA) hydrogels have attracted much attention due to their good biocompatibility, but the poor mechanical properties limit their application in bone reconstruction engineering. In this study, nano-hydroxyapatite (nHA) particles were added to GelMA hydrogels, and the performances of composite hydrogel scaffolds with different nHA contents were investigated in terms of rheological properties, light transmission properties, surface morphology, mechanical properties, and biocompatibility. The experimental results showed that the incorporation of nHA particles could effectively improve the printability and mechanical properties of the scaffolds, the scaffold fibers had better resistance to deformation, improved degradation rate, and biological experiments confirmed that nHA particles had no significant cytotoxicity. However, the addition of HA particles also reduced the light transmission properties of the slurry, and when its content exceeds a certain value, the hydrogel scaffolds show incomplete curing and eventually affect their test performance. The results can offer guidance and reference for the selection of ink and function for 3D printing bone repair scaffold.
{"title":"Study on properties of 3D-printed GelMA hydrogel scaffolds with different nHA contents","authors":"Yaocheng Wang, Chengxiong Lin","doi":"10.1177/08839115221119211","DOIUrl":"https://doi.org/10.1177/08839115221119211","url":null,"abstract":"Biological 3D printing is a reliable technology for 3D printing bone repair scaffolds with simple operation, high efficiency, and relatively low cost. Gelatin methacryloyl (GelMA) hydrogels have attracted much attention due to their good biocompatibility, but the poor mechanical properties limit their application in bone reconstruction engineering. In this study, nano-hydroxyapatite (nHA) particles were added to GelMA hydrogels, and the performances of composite hydrogel scaffolds with different nHA contents were investigated in terms of rheological properties, light transmission properties, surface morphology, mechanical properties, and biocompatibility. The experimental results showed that the incorporation of nHA particles could effectively improve the printability and mechanical properties of the scaffolds, the scaffold fibers had better resistance to deformation, improved degradation rate, and biological experiments confirmed that nHA particles had no significant cytotoxicity. However, the addition of HA particles also reduced the light transmission properties of the slurry, and when its content exceeds a certain value, the hydrogel scaffolds show incomplete curing and eventually affect their test performance. The results can offer guidance and reference for the selection of ink and function for 3D printing bone repair scaffold.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"1 1","pages":"392 - 405"},"PeriodicalIF":1.7,"publicationDate":"2022-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76548994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-07DOI: 10.1177/08839115221110284
H. Mahajan, V. Jadhao, Sachin M. Chandankar
The current work seeks to use Pullulan and Pluronic F-127 (PF-127), a new gel-forming material, for sildenafil citrate (SLC) intranasal delivery. The cold approach was used to develop an SLC-loaded in situ gel based on thermoreversible polymer PF-127 and mucoadhesive polymer Pullulan. In situ gel systems based on Pullulan responds intelligently to environmental stimuli like charge, pH, temperature, light, and redox. To achieve gelation at physiological temperature formulations were modified to have gelation temperatures lower than 34.1°C. Physical appearance and rheological measurements were used to calculate the temperature of gelation. With the addition of increasing quantities of Pullulan, the gelation temperatures fell (from 34.1°C for 8% w/v, 10% w/v, and 12% w/v 0.5% Pullulan). In the goat nasal mucosal membrane, Pullulan concentration increased the mucoadhesive force in terms of detachment stress. The results of drug permeation testing in vitro investigations over the goat nasal mucosa showed that utilizing an in situ gelling formulation with a Pullulan content of 0.5% or higher can greatly boost the effective penetration coefficient. The formulation was shown to be safe for the nasal mucosa after a histological investigation. Conclusively, Pullulan and PF-127 may be appropriate carriers for SLC intranasal administration.
{"title":"Pullulan and Pluronic F-127 based in situ gel system for intranasal delivery: Development, in vitro and in vivo evaluation","authors":"H. Mahajan, V. Jadhao, Sachin M. Chandankar","doi":"10.1177/08839115221110284","DOIUrl":"https://doi.org/10.1177/08839115221110284","url":null,"abstract":"The current work seeks to use Pullulan and Pluronic F-127 (PF-127), a new gel-forming material, for sildenafil citrate (SLC) intranasal delivery. The cold approach was used to develop an SLC-loaded in situ gel based on thermoreversible polymer PF-127 and mucoadhesive polymer Pullulan. In situ gel systems based on Pullulan responds intelligently to environmental stimuli like charge, pH, temperature, light, and redox. To achieve gelation at physiological temperature formulations were modified to have gelation temperatures lower than 34.1°C. Physical appearance and rheological measurements were used to calculate the temperature of gelation. With the addition of increasing quantities of Pullulan, the gelation temperatures fell (from 34.1°C for 8% w/v, 10% w/v, and 12% w/v 0.5% Pullulan). In the goat nasal mucosal membrane, Pullulan concentration increased the mucoadhesive force in terms of detachment stress. The results of drug permeation testing in vitro investigations over the goat nasal mucosa showed that utilizing an in situ gelling formulation with a Pullulan content of 0.5% or higher can greatly boost the effective penetration coefficient. The formulation was shown to be safe for the nasal mucosa after a histological investigation. Conclusively, Pullulan and PF-127 may be appropriate carriers for SLC intranasal administration.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":" 3","pages":"406 - 418"},"PeriodicalIF":1.7,"publicationDate":"2022-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72382176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-01DOI: 10.1177/08839115221106869
M. Hausen, A. Melero, J. Asami, L. M. Ferreira, Guilherme Borges Gomes da Silva, Mariana Cesar de Azeredo Bissoli, V. R. Marcato, B. D. Nani, P. Rosalen, S. M. Alencar, V. Botaro, D. Komatsu, A. Senna, E. Duek
An increasing interest in regenerative medicine has been an approach with natural products used for assorted skin treatments. Propolis from Apis mellifera species of bees have shown high acceptance due to antimicrobial and anti-inflammatory properties. However, just a few propolis types presents stronger effects in controlling inflammation. The current work describes an organic propolis recently isolated, named as OP6, that presented strong anti-inflammatory influences in vivo when associated with EDTA cross-linked hydrogel, used as a curative device in second-degree burns in a murine model. We developed a cellulose acetate hydrogel cross-linked with ethylenediaminetetraacetic dianhydride (HAC-EDTA) as a polymeric matrix for a bandage based on an ethanolic extract of propolis at 15%, 30%, and 60% (w/v) for treating second-degree burns. In vivo studies were carried out in Wistar rats divided into three groups: negative control (only lesion), positive control (lesion with HAC-EDTA film), and treatment group (lesion with the HAC-EDTA + OP6 at 15%, 30%, and 60%). Each group was randomized and equally subdivided into two subgroups according to the period of bandage wearing (7 and 14 days). Previous work of this research group selected the propolis OP6 sample source as the best candidate for the in vivo study. HAC-EDTA + OP6 15%, 30%, and 60% films demonstrated a concentration-dependent release rate, with the highest amount of propolis released after tests (484.3 mg) by HAC-EDTA enriched with the highest concentrated extract of propolis. HAC-EDTA + OP6 films were efficient in preventing infections, promoting lesion retraction, and tissue regeneration. The HAC-EDTA + OP6 30% treatment was more efficient, revealing a reduced inflammatory process and stimulating skin regeneration. The designed HAC-EDTA + propolis films were shown as promising tools for second-degree burns treatment, accelerating healing process to a full recovery tissue repair after 14 days.
{"title":"In vivo therapeutic evaluation of a cellulose acetate hydrogel cross linked with ethylenediaminetetraacetic-dianhydride containing propolis ethanolic-extract for treating burns","authors":"M. Hausen, A. Melero, J. Asami, L. M. Ferreira, Guilherme Borges Gomes da Silva, Mariana Cesar de Azeredo Bissoli, V. R. Marcato, B. D. Nani, P. Rosalen, S. M. Alencar, V. Botaro, D. Komatsu, A. Senna, E. Duek","doi":"10.1177/08839115221106869","DOIUrl":"https://doi.org/10.1177/08839115221106869","url":null,"abstract":"An increasing interest in regenerative medicine has been an approach with natural products used for assorted skin treatments. Propolis from Apis mellifera species of bees have shown high acceptance due to antimicrobial and anti-inflammatory properties. However, just a few propolis types presents stronger effects in controlling inflammation. The current work describes an organic propolis recently isolated, named as OP6, that presented strong anti-inflammatory influences in vivo when associated with EDTA cross-linked hydrogel, used as a curative device in second-degree burns in a murine model. We developed a cellulose acetate hydrogel cross-linked with ethylenediaminetetraacetic dianhydride (HAC-EDTA) as a polymeric matrix for a bandage based on an ethanolic extract of propolis at 15%, 30%, and 60% (w/v) for treating second-degree burns. In vivo studies were carried out in Wistar rats divided into three groups: negative control (only lesion), positive control (lesion with HAC-EDTA film), and treatment group (lesion with the HAC-EDTA + OP6 at 15%, 30%, and 60%). Each group was randomized and equally subdivided into two subgroups according to the period of bandage wearing (7 and 14 days). Previous work of this research group selected the propolis OP6 sample source as the best candidate for the in vivo study. HAC-EDTA + OP6 15%, 30%, and 60% films demonstrated a concentration-dependent release rate, with the highest amount of propolis released after tests (484.3 mg) by HAC-EDTA enriched with the highest concentrated extract of propolis. HAC-EDTA + OP6 films were efficient in preventing infections, promoting lesion retraction, and tissue regeneration. The HAC-EDTA + OP6 30% treatment was more efficient, revealing a reduced inflammatory process and stimulating skin regeneration. The designed HAC-EDTA + propolis films were shown as promising tools for second-degree burns treatment, accelerating healing process to a full recovery tissue repair after 14 days.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"367 1","pages":"343 - 355"},"PeriodicalIF":1.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82583159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guided tissue regeneration (GTR) membranes not only can hamper undesirable tissues down-growth into the defects but also can selectively promote the in-growth of regenerative bone tissue, playing a critical role in periodontal regeneration. Herein, a bi-layered electrospun membrane with different sized pores was designed and fabricated by adjusting electrospinning parameters combing with facile two-step electrospinning. The small-sized pore layer (SL) as occlusive layer consisted of electrospun poly (lactic-co-glycolic acid) (PLGA) nanofibers, while the macroporous osteoconductive layer (ML) was attained via introducing the nano-hydroxyapatite (nHA) particles into PLGA nanofibers during electrospinning. Morphological results such as surface topography, nanofiber size, and pore size distribution, showed that the SL exhibited a dense structure with pore size mainly from 4 to 7 μm. In contrast, the ML possessed a loosely packed structure with pore size mainly from 20 to 28 μm, which was beneficial to the infiltration of the cells. Fourier transform infrared spectroscopy (FTIR), Energy dispersive spectrometer (EDS), and X-ray diffractometry (XRD) results showed that nHA particles were evenly loaded in PLGA nanofibers. In vitro biodegradation tests suggested that the bi-layered membrane possessed a proper degradation timeframe, which must function for at least 4 to 6 weeks. The cell experiments indicated that the bi-layered electrospun membrane possessed good cytocompatibility and proved the effective barrier potency of the small-sized pore layer. Furthermore, as revealed by the alkaline phosphate activity test, the PLGA/nHA layer possessed an improved osteogenic capacity for Human osteosarcoma cells (MG63). These results indicate that the bi-layered electrospun membrane may have potential for periodontal tissue regeneration. Graphical Abstract
{"title":"Bi-layered PLGA electrospun membrane with occlusive and osteogenic properties for periodontal regeneration","authors":"Meiling Zhong, Jixia Lin, Zhimin He, Wuchao Wu, De-hui Ji, Richao Zhang, Jiali Zhang","doi":"10.1177/08839115221095257","DOIUrl":"https://doi.org/10.1177/08839115221095257","url":null,"abstract":"Guided tissue regeneration (GTR) membranes not only can hamper undesirable tissues down-growth into the defects but also can selectively promote the in-growth of regenerative bone tissue, playing a critical role in periodontal regeneration. Herein, a bi-layered electrospun membrane with different sized pores was designed and fabricated by adjusting electrospinning parameters combing with facile two-step electrospinning. The small-sized pore layer (SL) as occlusive layer consisted of electrospun poly (lactic-co-glycolic acid) (PLGA) nanofibers, while the macroporous osteoconductive layer (ML) was attained via introducing the nano-hydroxyapatite (nHA) particles into PLGA nanofibers during electrospinning. Morphological results such as surface topography, nanofiber size, and pore size distribution, showed that the SL exhibited a dense structure with pore size mainly from 4 to 7 μm. In contrast, the ML possessed a loosely packed structure with pore size mainly from 20 to 28 μm, which was beneficial to the infiltration of the cells. Fourier transform infrared spectroscopy (FTIR), Energy dispersive spectrometer (EDS), and X-ray diffractometry (XRD) results showed that nHA particles were evenly loaded in PLGA nanofibers. In vitro biodegradation tests suggested that the bi-layered membrane possessed a proper degradation timeframe, which must function for at least 4 to 6 weeks. The cell experiments indicated that the bi-layered electrospun membrane possessed good cytocompatibility and proved the effective barrier potency of the small-sized pore layer. Furthermore, as revealed by the alkaline phosphate activity test, the PLGA/nHA layer possessed an improved osteogenic capacity for Human osteosarcoma cells (MG63). These results indicate that the bi-layered electrospun membrane may have potential for periodontal tissue regeneration. Graphical Abstract","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"337 1","pages":"284 - 298"},"PeriodicalIF":1.7,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72849629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-22DOI: 10.1177/08839115221106700
J. Bakshi, M. Mehra, S. Grewal, D. Dhingra, S. Kumari
In the present study, the anti-diabetic and antimicrobial properties of berberine were improved using non-ionic guar gum and ionic acacia gum as nanocarriers. Berberine loaded guar-acacia gum nanocomplexes were synthesized by employing ionic complexation method. The formulation was characterized by dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), Scanning electron microscopy (SEM) and evaluated for in vitro dissolution study, anti-diabetic activity and antimicrobial activity. The optimized berberine loaded guar-acacia gum nanocomplexes had a particle size of 290.2 nm as indicated by DLS and drug entrapment efficiency of 96.5%. Morphological analysis revealed that berberine nanocomplexes were spherical-shaped with a smooth surface and size in the range of 100–250 nm. Moreover, berberine loaded guar-acacia nanocomplexes showed good stability and controlled released property in vitro. Antimicrobial activity against bacterial strains and fungal strains demonstrated the higher antimicrobial potential of berberine loaded gum nanocomplexes than gum nanocomplexes (blank) and pure berberine as indicated by the greater zone of inhibition diameter. In vitro anti-diabetic assessment showed higher percentage inhibition of the α-amylase enzyme by berberine loaded gum nanocomplexes as compared to pure berberine and blank nanocomplexes. In conclusion, the improved biological potency of berberine upon encapsulation into gum nanocomplexes indicates that berberine loaded guar-acacia gum nanocomplexes can be used as a promising candidate against diabetes and pathogenic microorganisms in the near future.
{"title":"Synthesis, characterization and evaluation of in vitro antimicrobial and anti-diabetic activity of berberine encapsulated in guar-acacia gum nanocomplexes","authors":"J. Bakshi, M. Mehra, S. Grewal, D. Dhingra, S. Kumari","doi":"10.1177/08839115221106700","DOIUrl":"https://doi.org/10.1177/08839115221106700","url":null,"abstract":"In the present study, the anti-diabetic and antimicrobial properties of berberine were improved using non-ionic guar gum and ionic acacia gum as nanocarriers. Berberine loaded guar-acacia gum nanocomplexes were synthesized by employing ionic complexation method. The formulation was characterized by dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), Scanning electron microscopy (SEM) and evaluated for in vitro dissolution study, anti-diabetic activity and antimicrobial activity. The optimized berberine loaded guar-acacia gum nanocomplexes had a particle size of 290.2 nm as indicated by DLS and drug entrapment efficiency of 96.5%. Morphological analysis revealed that berberine nanocomplexes were spherical-shaped with a smooth surface and size in the range of 100–250 nm. Moreover, berberine loaded guar-acacia nanocomplexes showed good stability and controlled released property in vitro. Antimicrobial activity against bacterial strains and fungal strains demonstrated the higher antimicrobial potential of berberine loaded gum nanocomplexes than gum nanocomplexes (blank) and pure berberine as indicated by the greater zone of inhibition diameter. In vitro anti-diabetic assessment showed higher percentage inhibition of the α-amylase enzyme by berberine loaded gum nanocomplexes as compared to pure berberine and blank nanocomplexes. In conclusion, the improved biological potency of berberine upon encapsulation into gum nanocomplexes indicates that berberine loaded guar-acacia gum nanocomplexes can be used as a promising candidate against diabetes and pathogenic microorganisms in the near future.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"46 1","pages":"233 - 251"},"PeriodicalIF":1.7,"publicationDate":"2022-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74463747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-17DOI: 10.1177/08839115221104072
Lai Suo, Zhijun Xue, Puyu Wang, Hongshan Wu, Yao Chen, Jing Shen
Oral and maxillofacial tumors, trauma and infections are the main causes of jaw defects, whose clinical treatment is very complicated. With the development of biological tissue engineering, many biological materials have been widely used in various fields of stomatology, and they play a very important role in the repair and replacement of maxillofacial bone defects. In this study, we intended to prepare a graphene oxide/hyaluronic acid/chitosan (GO/HA/CS) composite hydrogel with different mass ratios of GO: 0.1% (0.1% GO/HA/CS), 0.25% (0.25% GO/HA/CS), 0.5% (0.5% GO/HA/CS), and 1% (1% GO/HA/CS), prepare it into a multilayered and stable composite scaffold through 3D-printing technology, observe the surface morphology of the composite scaffold through scanning electron microscopy (SEM), and then test its physical and chemical properties, mechanical properties, water swelling rate, in vitro degradation and other material properties. Moreover, the biological performance of the GO/HA/CS composite scaffold was studied through experiments, such as cell morphology observation, cell adhesion, cell proliferation, and live-dead cell staining. The results showed that through chemical cross-linking and 3D-printing technology, a porous (pore size: 450–580 μm) and multilayered GO/HA/CS biological scaffold could be successfully constructed, and its surface was an interconnected microporous structure, and the porosity decreased (94%−40%) gradually with the increase of GO. Meanwhile, with the change in GO concentration, some mechanical properties of the scaffold could be improved, such as water swelling rate, degradation rate, and elastic modulus. In addition, the composite scaffold with the appropriate amount of GO had almost no cytotoxicity and could promote cell growth and proliferation, especially 0.25% GO/HA/CS composite scaffold. Consequently, the 0.25% GO/HA/CS composite scaffold had excellent biological material properties and good biocompatibility with osteoblasts, which may provide a new idea for the repair of jaw defects.
{"title":"Improvement of osteogenic properties using a 3D-printed graphene oxide/hyaluronic acid/chitosan composite scaffold","authors":"Lai Suo, Zhijun Xue, Puyu Wang, Hongshan Wu, Yao Chen, Jing Shen","doi":"10.1177/08839115221104072","DOIUrl":"https://doi.org/10.1177/08839115221104072","url":null,"abstract":"Oral and maxillofacial tumors, trauma and infections are the main causes of jaw defects, whose clinical treatment is very complicated. With the development of biological tissue engineering, many biological materials have been widely used in various fields of stomatology, and they play a very important role in the repair and replacement of maxillofacial bone defects. In this study, we intended to prepare a graphene oxide/hyaluronic acid/chitosan (GO/HA/CS) composite hydrogel with different mass ratios of GO: 0.1% (0.1% GO/HA/CS), 0.25% (0.25% GO/HA/CS), 0.5% (0.5% GO/HA/CS), and 1% (1% GO/HA/CS), prepare it into a multilayered and stable composite scaffold through 3D-printing technology, observe the surface morphology of the composite scaffold through scanning electron microscopy (SEM), and then test its physical and chemical properties, mechanical properties, water swelling rate, in vitro degradation and other material properties. Moreover, the biological performance of the GO/HA/CS composite scaffold was studied through experiments, such as cell morphology observation, cell adhesion, cell proliferation, and live-dead cell staining. The results showed that through chemical cross-linking and 3D-printing technology, a porous (pore size: 450–580 μm) and multilayered GO/HA/CS biological scaffold could be successfully constructed, and its surface was an interconnected microporous structure, and the porosity decreased (94%−40%) gradually with the increase of GO. Meanwhile, with the change in GO concentration, some mechanical properties of the scaffold could be improved, such as water swelling rate, degradation rate, and elastic modulus. In addition, the composite scaffold with the appropriate amount of GO had almost no cytotoxicity and could promote cell growth and proliferation, especially 0.25% GO/HA/CS composite scaffold. Consequently, the 0.25% GO/HA/CS composite scaffold had excellent biological material properties and good biocompatibility with osteoblasts, which may provide a new idea for the repair of jaw defects.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"3 1","pages":"267 - 283"},"PeriodicalIF":1.7,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90295419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-10DOI: 10.1177/08839115221104074
Linli Li, Fengjuan Wang
Anethum graveolens extract (AGE) is known for its anti-inflammatory, antioxidative, and antibacterial activities. As wound infection, hyperactivity of inflammatory responses, and high oxidative stress are the leading causes of delayed wound healing, we were encouraged to design a delivery vehicle for AGE to develop a potential wound dressing material. In the current study, AGE was incorporated into the polyvinyl alcohol (PVA) scaffolds matrix via the electrospinning method. Various characterization methods were applied to assess the physicochemical and biological properties of the dressings. Cell culture studies with fibroblast cell line showed that AGE-loaded dressings could significantly promote cell viability under normal and oxidative stress conditions. The prepared wound dressings’ wound healing and anti-inflammatory properties were investigated on an excisional injury rat model. Wound healing assay showed that AGE-delivering wound dressings could significantly improve the wound healing response, as evidenced by a significantly higher rate of wound closure, epithelial thickness, and collagen deposition. Gene expression analysis revealed that the produced dressings downregulated inflammation-associated genes such as IL-1β and NFK-β. This preliminary research suggests the potential applicability of AGE-loaded PVA dressings in the clinic.
{"title":"Wound healing and anti-inflammatory effects of Anethum graveolens extract loaded in PVA fibers: An in vitro and in vivo study","authors":"Linli Li, Fengjuan Wang","doi":"10.1177/08839115221104074","DOIUrl":"https://doi.org/10.1177/08839115221104074","url":null,"abstract":"Anethum graveolens extract (AGE) is known for its anti-inflammatory, antioxidative, and antibacterial activities. As wound infection, hyperactivity of inflammatory responses, and high oxidative stress are the leading causes of delayed wound healing, we were encouraged to design a delivery vehicle for AGE to develop a potential wound dressing material. In the current study, AGE was incorporated into the polyvinyl alcohol (PVA) scaffolds matrix via the electrospinning method. Various characterization methods were applied to assess the physicochemical and biological properties of the dressings. Cell culture studies with fibroblast cell line showed that AGE-loaded dressings could significantly promote cell viability under normal and oxidative stress conditions. The prepared wound dressings’ wound healing and anti-inflammatory properties were investigated on an excisional injury rat model. Wound healing assay showed that AGE-delivering wound dressings could significantly improve the wound healing response, as evidenced by a significantly higher rate of wound closure, epithelial thickness, and collagen deposition. Gene expression analysis revealed that the produced dressings downregulated inflammation-associated genes such as IL-1β and NFK-β. This preliminary research suggests the potential applicability of AGE-loaded PVA dressings in the clinic.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"160 1","pages":"299 - 315"},"PeriodicalIF":1.7,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77852695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-07DOI: 10.1177/08839115221104071
Simin Nazarnezhad, M. Salehi, H. Samadian, Arian Ehtermi, N. Kasaiyan, H. Khastar, Ghasem Abbaszadeh-Goudarzi, Nariman Rezaei Kolarijani, Hodays Yeganehfard, H. Ziaei
The current study’s main aim was to fabricate and evaluate alginate (Alg) hydrogel containing retinoic acid (RA) as wound healing materials. Different RA concentrations (2, 10, and 50% w/w) were incorporated into the hydrogel. The results showed that the prepared hydrogels had a porous structure with a pore size of 69.69 ± 22.1 µm for pure Alg hydrogel and 78.44 ± 27.8 µm for Alg/RA hydrogel. The swelling measurement showed that the hydrogels swelled up to 65% and the incorporation of RA reduced the degree of swelling . The in vitro studies confirmed the hemo- and biocompatibility of the Alg/RA 2% and increasing the RA concentration induced hemolysis and toxic effects. The animal studies showed that the lowest RA concentration resulted in the best treatment outcome while increasing the RA concentration suppressed the healing process. In conclusion, these results showed that RA induced wound healing process at low concentration, and the prepared hydrogel could be used as the wound healing materials.
{"title":"In vitro and in vivo evaluation of porous alginate hydrogel containing retinoic acid for skin wound healing applications","authors":"Simin Nazarnezhad, M. Salehi, H. Samadian, Arian Ehtermi, N. Kasaiyan, H. Khastar, Ghasem Abbaszadeh-Goudarzi, Nariman Rezaei Kolarijani, Hodays Yeganehfard, H. Ziaei","doi":"10.1177/08839115221104071","DOIUrl":"https://doi.org/10.1177/08839115221104071","url":null,"abstract":"The current study’s main aim was to fabricate and evaluate alginate (Alg) hydrogel containing retinoic acid (RA) as wound healing materials. Different RA concentrations (2, 10, and 50% w/w) were incorporated into the hydrogel. The results showed that the prepared hydrogels had a porous structure with a pore size of 69.69 ± 22.1 µm for pure Alg hydrogel and 78.44 ± 27.8 µm for Alg/RA hydrogel. The swelling measurement showed that the hydrogels swelled up to 65% and the incorporation of RA reduced the degree of swelling . The in vitro studies confirmed the hemo- and biocompatibility of the Alg/RA 2% and increasing the RA concentration induced hemolysis and toxic effects. The animal studies showed that the lowest RA concentration resulted in the best treatment outcome while increasing the RA concentration suppressed the healing process. In conclusion, these results showed that RA induced wound healing process at low concentration, and the prepared hydrogel could be used as the wound healing materials.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"115 1","pages":"332 - 342"},"PeriodicalIF":1.7,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87817394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-07DOI: 10.1177/08839115221098058
Phuong Le Thi, T. Tran, H. Luu, Dieu Linh Tran, T. H. Thi, D. Nguyen
Injectable hydrogels offer a wide range of attractive benefits in drug delivery applications, such as non-invasive administration, easy drug incorporation and locally controlled release at the target sites. Herein, we designed a simple and efficient method to prepare injectable hydrogels composed of gelatin and cyclodextrin (CD) for high loading capacity of hydrophobic drugs. The hydrogels were formed by thiol-functionalized gelatin (GSH) and βCD-vinyl sulfone (βCD-VS) as cross-linker, via thiol-ene “click” chemistry. Hydrogels comprising of different cross-linker feed amount were investigated in terms of their physico-chemical properties, such as gelation time, mechanical strength, swelling ratio, porosity and degradation rates. For the use as a drug delivery vehicle, dexamethasone (DEX), a commonly anti-inflammatory, immunosuppressive but poorly water soluble drug was chosen to show the high drug loading capacity and prolonged drug release of hydrogels. The drug release was found to be depended on the concentration of βCD-VS due to the drug-CD interaction. In vitro cytotoxicity experiment also showed the cell compatibility of these hydrogels against human dermal fibroblasts. In summary, we expect this gelatin-CD “click” hydrogel will be a promising candidate for localized and long-term delivery of hydrophobic drugs. Graphical Abstract
可注射水凝胶在药物递送应用中提供了广泛的吸引力，例如非侵入性给药，易于药物合并和在目标部位局部控制释放。本研究设计了一种简单、高效的方法制备由明胶和环糊精(CD)组成的可注射型水凝胶，以提高疏水药物的负载能力。以巯基功能化明胶(GSH)为交联剂，以β cd -乙烯基砜(βCD-VS)为交联剂，通过巯基“咔嗒”反应形成水凝胶。研究了不同交联剂投加量的水凝胶的理化性质，如凝胶时间、机械强度、溶胀率、孔隙率和降解率。地塞米松(dexamethasone, DEX)是一种常见的抗炎、免疫抑制但水溶性较差的药物，具有较高的载药量和较长的水凝胶释药时间。由于药物- cd相互作用，药物释放依赖于βCD-VS的浓度。体外细胞毒性实验也显示了水凝胶对人真皮成纤维细胞的细胞相容性。总之，我们预计这种明胶- cd“点击”水凝胶将成为一种有希望的局部和长期递送疏水药物的候选者。图形抽象
{"title":"In situ forming gelatin: Cyclodextrin hydrogels prepared by “click chemistry” to improve the sustained release of hydrophobic drugs","authors":"Phuong Le Thi, T. Tran, H. Luu, Dieu Linh Tran, T. H. Thi, D. Nguyen","doi":"10.1177/08839115221098058","DOIUrl":"https://doi.org/10.1177/08839115221098058","url":null,"abstract":"Injectable hydrogels offer a wide range of attractive benefits in drug delivery applications, such as non-invasive administration, easy drug incorporation and locally controlled release at the target sites. Herein, we designed a simple and efficient method to prepare injectable hydrogels composed of gelatin and cyclodextrin (CD) for high loading capacity of hydrophobic drugs. The hydrogels were formed by thiol-functionalized gelatin (GSH) and βCD-vinyl sulfone (βCD-VS) as cross-linker, via thiol-ene “click” chemistry. Hydrogels comprising of different cross-linker feed amount were investigated in terms of their physico-chemical properties, such as gelation time, mechanical strength, swelling ratio, porosity and degradation rates. For the use as a drug delivery vehicle, dexamethasone (DEX), a commonly anti-inflammatory, immunosuppressive but poorly water soluble drug was chosen to show the high drug loading capacity and prolonged drug release of hydrogels. The drug release was found to be depended on the concentration of βCD-VS due to the drug-CD interaction. In vitro cytotoxicity experiment also showed the cell compatibility of these hydrogels against human dermal fibroblasts. In summary, we expect this gelatin-CD “click” hydrogel will be a promising candidate for localized and long-term delivery of hydrophobic drugs. Graphical Abstract","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"36 12 1","pages":"252 - 266"},"PeriodicalIF":1.7,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90664018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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