Journal of Biomaterials Applications最新文献

Building on our innovative approach to combatting cancer, this study explores the development of a sophisticated hybrid nanocarrier system leveraging the unique properties of allyl oxide cucurbit[6]uril with galactose clusters (AOQ[6]@Gal) to modify ZIF-8 nanoparticles. These nanoparticles are designed to encapsulate and efficiently deliver the anticancer drugs doxorubicin (DOX) and curcumin (CUR), enhancing their water solubility and stability, while also providing active targeting towards hepatocellular carcinoma cells. The comprehensive characterization of AOQ[6]@Gal@ZIF-8@Drug nanoparticles revealed promising outcomes, including drug loading efficiencies of 9.7% for DOX and 8.3% for CUR, alongside a pH-responsive release profile that ensures effective drug delivery in the tumor microenvironment. Cytotoxicity studies underscored the hybrid system's superior safety profile, exhibiting minimal toxicity towards normal hepatocytes HL7702 and pronounced cytotoxic effects against hepatocellular carcinoma cells HepG2. These results highlight the hybrid nanocarrier's potential as a targeted, efficient, and safe platform for the delivery of chemotherapy agents in the treatment of liver cancer.

In this study, silicon phthalocyanine dichloride (SiCl₂Pc) was successfully encapsulated in β-cyclodextrin (β-CD) and hydroxy-propyl-β-cyclodextrin (HP-β-CD) using the kneading method. Dynamic Light Scattering (DLS) demonstrated complexes of various hydrodynamic diameters with moderate stability in aqueous solutions. Their structural characterization by Infrared Spectroscopy (FT- IR) indicated that a part of phthalocyanine is located inside the cyclodextrin cavity. Both photophysical and photochemical studies showed that phthalocyanine's encapsulation in cyclodextrins increased its aqueous solubility. The photodynamic studies against A431 cancer cell line indicated that the complexes are more effective than pure SiCl₂Pc. Pure SiCl₂Pc's photodynamic effect is characterized as dose-dependent, whereas both complexes presented a biphasic dose-response photodynamic effect. For the highest energy dose of 3.24 J/cm², pure SiCl₂Pc induced mild cell toxicity. SiCl₂Pc-β-CD complex was the most promising photosensitizer, exhibiting the highest photodynamic effect when irradiated at 2.16 J/cm².

Drospirenone (DROP) is a highly effective, low-toxicity, safe new generation progestin that counteracts estrogen-related sodium retention, is well tolerated, and has a positive effect on premenstrual syndrome (PMS). However, the low water solubility of DROP and its chemical instability resulted in low bioavailability. In this study, we developed a two-step delivery system to enhance drospirenone's solubility and stability. We prepared a drospirenone liposome complex to optimize the encapsulation process and achieve an encapsulation efficiency of (84.9 ± 0.73) %, with an 878-fold increase in solubility under optimal conditions. To address the instability of high drug-loading liposomes, we immobilized the drospirenone liposome inclusion complex using a cellulose-based hydrogel. The system achieved uniform loading of liposomes in the hydrogel, as confirmed by SEM and FTIR analysis. 0.5 g hydrogel can be loaded with up to 96.48 mg drospirenone, and the encapsulation efficiency is (80.4 ± 1.17%). It was indicating the potential for wider application of drospirenone with enhanced water solubility and improved stability. At the same time, it also provides support for sustained-release systems or large dose drug delivery.

Fabricating scaffolds using three-dimensional (3D) printing is an emerging approach in tissue engineering (TE), where filaments with a controlled arrangement are printed. Using fused deposition modeling in bone replacement enables the simulation of bone structure. However, the microenvironment created by the scaffold must meet specific requirements. These requirements aim to create an environment that promotes adhesion, proliferation, differentiation, and cell migration. One of the challenges in creating polylactic acid scaffolds is controlling the degradation rate to match the target tissue. This study investigates the degradation of scaffolds with different geometries and the relationship between scaffolds' geometry and degradation rate. These scaffolds are made of polylactic acid and prepared using 3D printing. The lattice geometry was exposed to acidic media with varying pH levels for 1 month, and pH2 was selected for all geometries for further investigation. The five selected geometries were then immersed in the desired acid for 2 months, and measurements were taken for wet weight, dry weight, morphology, molecular weight, and crystallinity during degradation. The results showed that the hexagonal sample had a 1.5% increase in wet weight, and the gyroid sample had a 1.2% increase, indicating that the wavy shapes had a higher fluid-holding capacity. The degradation analysis indicated that the hexagonal geometry had accelerated degradation compared to the other geometries. Based on these findings, it can be concluded that filament separation not only results in rapid cooling and prevents the recovery of the crystalline arrangement but also increases the surface area to volume ratio, allowing for more acid penetration and faster degradation. Finally, mechanical properties and in vitro evaluation were assessed for three selected geometries. On the 60th day, the hexagonal scaffold had the highest elastic modulus value of 105 ± 0.45 MPa, while the gyroid scaffold had the lowest value of 58.8 ± 0.40 MPa. The lattice scaffold had the highest amount of cell attachment, with 210.88 ± 0.35 cells surviving after 24 hours and 94.01 ± 0.18 cells surviving after 72 hours. These high viability rates indicate that the three scaffolds with the selected geometries are suitable for promoting cell growth.

Collagens are abundant structural proteins found in both mammalian and marine species, and attractive biomaterials used in various fields. Jellyfish collagen-based products have become increasingly popular because of their clinically proven health benefits such as the effects of skin wound healing and immune stimulation. To develop detection tools for jellyfish collagen, we generated four monoclonal antibodies, MCOL1, 2, 3, and 4, by immunizing mice with moon jellyfish collagen. The nucleotide and amino acid sequences of the variable regions of the monoclonal antibodies were determined. The antibody-binding kinetics toward collagens from moon jellyfish were evaluated using a surface plasmon resonance (SPR) biosensor, and the binding specificity was evaluated in comparison with binding to collagens from edible jellyfish, fish scales, and pig and cow skins. MCOL1, 3, and 4 specifically bound to moon jellyfish collagen, whereas MCOL2 bound to both moon and edible jellyfish collagens. Considering the results showing that the SPR responses of MCOL2 binding were greater than those seen with the other antibodies, MCOL2 could recognize the common and repetitive sequences of the two jellyfish collagens. Therefore, this monoclonal antibody will be most applicable for detecting jellyfish collagen.

Osteoarthritis (OA) presents a significant global health burden, necessitating innovative therapeutic strategies to address its multifaceted challenges. This study explores the potential of Citrus trifoliata extract-loaded chitosan nanoparticles (CTECNPs) as a novel treatment modality for OA. The encapsulation of Citrus trifoliata extract (CTE) within chitosan nanoparticles offers advantages such as enhanced bioavailability, sustained release kinetics, and targeted delivery to affected joints. In vitro evaluations demonstrate the biocompatibility and anti-inflammatory properties of CTECNPs, with significant anti-inflammatory and antioxidative effects observed. Moreover, in vivo studies in an OA-induced mouse model reveal promising therapeutic outcomes, including improvements in histological features and locomotor function. These findings highlight the potential of CTECNPs as a promising therapeutic approach for OA, offering hope for improved patient outcomes and quality of life. Further research is warranted to elucidate additional signaling pathways and potential synergistic effects of CTECNPs in OA management.

Due to the absence of blood vessels, cartilage exhibits extremely limited self-repair capacity. Currently, repairing laryngeal cartilage defects, resulting from conditions such as laryngeal tumors, injury, and congenital structural abnormalities, remains a significant challenge in the Department of Otolaryngology, Head and Neck Surgery. Previous research has often focused on enhancing the mechanical properties of synthetic materials. However, their low biological activity and weak cell adhesion necessitate compensatory measures. This study aims to capitalize on the advantages of natural materials in cartilage tissue engineering. Sodium alginate, gelatin, tannic acid, and calcium chloride were utilized to prepare bioinks through cross-linking for application in 3D printing cartilage scaffolds. Bone marrow mesenchymal stem cells with multidirectional differentiation potential were chosen as seed cells, with appropriate growth factors incorporated to promote their differentiation into cartilage during in vitro culture. The scaffold laden cells was subsequently implanted into rabbit thyroid cartilage plate defects at the appropriate time. HE staining, toluidine blue staining, Masson staining, and collagen type II staining were employed to assess cartilage defect repair at 4, 8, and 12 weeks, respectively. Results demonstrated that scaffolds made from natural materials could emulate the mechanical properties of fresh cartilage with commendable biocompatibility. Stained sections further confirmed the efficacy of the composite hydrogel scaffolds identified in this study in promoting rabbit thyroid cartilage plate restoration. In summary, this study successfully fabricated a natural material scaffold for rabbit laryngeal cartilage tissue engineering, thereby furnishing a new idea and experience for the clinical application of laryngeal cartilage defect reconstruction.

Bacterial infection has always been a severe challenge for mankind. The use of antibacterial nonwoven materials provides a lot of convenience in daily life and clinical practice grammar revision, it has become an important solution to avoid bacterial infection in clinical and daily life. This review systematically examines the spin bonding, melt blown, hydroneedling and electrospinning methods of nonwoven fabrication materials, and summarizes the antibacterial nonwoven materials fabrication methods. Finally, the review discusses the applications of antibacterial nonwoven materials for medical protection, external medical and healthcare, external circulation medical care implantable medical and healthcare and intelligent protection and detection. This comprehensive overview aims to provide valuable insights for the advancement of antibacterial nonwoven materials in the domain of medicine and health care. In the future, antibacterial nonwoven materials are expected to evolve towards biodegradability, composite materials, functionalization, minimally invasive techniques, diversification, and intelligence, thereby holding immense potential in healthcare.

Although KI24RGDS peptide hydrogel that acts as a cell adhesion has been reported to repair tissue in meniscus injury, its effect on tendon injuries remains unknown. The purpose of this study was to clarify the effect of KI24RGDS for tendon repair based on histological and biomechanical evaluation. After introducing defects (length: 10 mm; width: 3 mm) at the centers of rabbits' patellar tendons, and the KI24RGDS group was implanted with KI24RGDS and observed for 8 weeks. KI24RGDS implantation resulted in limited tendon elongation and better histological scores with uniformed collagen fiber orientation and early vascularization. The failure load of the patellar tendon was higher in the KI24RGDS group than that in the defect group (p < 0.05) and no significant difference with the control group (intact patellar tendon) at 8 weeks postoperatively. In conclusion, KI24RGDS administration might have therapeutic potential for tendon injuries by accelerating collagen remodeling.

Sciatic nerve damage, a common condition affecting approximately 2.8% of the US population, can lead to significant disability due to impaired nerve signal transmission, resulting in loss of sensation and motor function in the lower extremities. In this study, a neural guidance channel was developed by rolling a nanofibrous scaffold produced via electrospinning. The scaffold's microstructure, biocompatibility, biodegradation rate, porosity, mechanical properties, and hemocompatibility were evaluated. Platelet-rich plasma (PRP) activated with 30,000 allogeneic Schwann cells (SCs) was injected into the lumen of the channels following implantation into a rat model of sciatic nerve injury. Recovery of motor function, sensory function, and muscle re-innervation was assessed using the sciatic function index (SFI), hot plate latency time, and gastrocnemius muscle wet weight loss. Results showed mean hot plate latency times of Autograft: 7.03, PCL/collagen scaffolds loaded with PRP and SCs (PCLCOLPRPSCs): 8.34, polymer-only scaffolds (PCLCOL): 10.66, and untreated animals (Negative Control): 12.00. The mean SFI values at week eight were Autograft: -49.30, PCLCOLPRPSCs: -64.29, PCLCOL: -75.62, and Negative Control: -77.14. The PCLCOLPRPSCs group showed a more negative SFI compared to the Autograft group but performed better than both the PCLCOL and Negative Control groups. These findings suggest that the developed strategy enhanced sensory and functional recovery compared to the negative control and polymer-only scaffold groups.