Journal of biotechnology最新文献_第2页

Combinatorial metabolic engineering strategy of precursor pools for the yield improvement of spinosad in Saccharopolyspora spinosa 采用前体池组合代谢工程策略提高刺槐多糖的产量。

IF 4.1 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2024-11-02 DOI: 10.1016/j.jbiotec.2024.10.010

Li Cao , Xirong Liu , Danlu Yang , Ziyuan Xia , Zirui Dai , Lin Sun , Jing Fang , Zirong Zhu , Duo Jin , Jie Rang , Shengbiao Hu , Liqiu Xia

Spinosad is an insecticide produced by Saccharopolyspora spinosa, and its larvicidal activity is considered a promising approach to combat crop pests. The aim of this study was to enhance the synthesis of spinosad through increasing the supply of acyl-CoAs precursor by the following steps. (i) Engineering the β-oxidation pathway by overexpressing key genes within the pathway to promote the synthesis of spinosad. The results showed that the overexpression of fadD, fadE, and fadA1 genes, as well as the co-expression of fadA1 and fadE genes, increased the yield of spinosad by 0.36-fold, 0.89-fold, 0.75-fold and 1.25-fold respectively. (ii) Employing combinatorial engineering of the β-oxidation pathway and ACC/PCC pathway to promote the synthesis of spinosad. The results showed that the co-expression of fadE and pccA, as well as accC and fadE, resulted in a 1.77-fold and 1.43-fold increase in spinosad production respectively. (iii) When exogenous triacylglycerol was added to the fermentation medium, the solely engineering of the β-oxidation pathway increased the yield of spinosad by 7.13-fold, reaching 427.23 mg/L. While the combinatorial engineering of both the β-oxidation pathway and ACC/PCC pathway increased the yield of spinosad by 9.61-fold, reaching 625.17 mg/L, and further optimization of the culture medium resulted in an even higher yield of spinosad, reaching 1293.43 mg/L. The results of this study indicate that the above combination strategy can promote the efficient biosynthesis of spinosad.

刺五加是由刺五加孢属（Saccharopolyspora spinosa）产生的一种杀虫剂，其杀幼虫活性被认为是一种很有前景的防治农作物害虫的方法。本研究的目的是通过以下步骤增加酰基-CoAs 前体的供应，从而提高刺五加的合成。(i) 通过过表达β-氧化途径中的关键基因，对该途径进行工程化改造，以促进尖孢噻吨的合成。结果表明，过表达 fadD、fadE 和 fadA1 基因，以及共同表达 fadA1 和 fadE 基因，可使尖孢霉素的产量分别提高 0.36 倍、0.89 倍、0.75 倍和 1.25 倍。(ii) 采用β-氧化途径和 ACC/PCC 途径的组合工程来促进刺五加的合成。结果表明，当 fadE 和 pccA 以及 accC 和 fadE 共同表达时，尖孢苷的产量分别增加了 1.77 倍和 1.43 倍。(iii) 当发酵培养基中加入外源三酰甘油时，单纯的 β 氧化途径工程可使尖孢霉素的产量增加 7.13 倍，达到 427.23 毫克/升。而β-氧化途径和ACC/PCC途径的组合工程则使尖霉素的产量增加了9.61倍，达到625.17mg/L，进一步优化培养基后，尖霉素的产量更高，达到1293.43mg/L。该研究结果表明，上述组合策略可促进尖孢霉素的高效生物合成。

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引用次数: 0

An overview of engineering microbial production of nicotinamide mononucleotide 烟酰胺腺嘌呤单核苷酸微生物工程生产概述。

IF 4.1 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2024-11-02 DOI: 10.1016/j.jbiotec.2024.10.014

Boting Li, Xiangfeng Meng, Weifeng Liu

As the human body gradually ages, the cellular level of NAD⁺ will decline, which has been found to be related to a variety of age-related diseases. As a precursor of NAD⁺, NMN is able to effectively promote the synthesis of NAD⁺ with no significant side effects. Microbial production of NMN holds the potential to lower the production cost and facilitate its wide application. In this review, based on the metabolic pathway of NAD⁺, we summarize recent advances of metabolic engineering strategies for NMN biosynthesis. An outlook for future optimization to improve NMN production is also discussed.

随着人体逐渐衰老，细胞中的 NAD+ 水平会下降，这已被发现与多种老年相关疾病有关。作为 NAD+ 的前体，NMN 能够有效促进 NAD+ 的合成，且无明显副作用。微生物生产 NMN 有可能降低生产成本，促进其广泛应用。本综述以 NAD+ 的代谢途径为基础，总结了 NMN 生物合成代谢工程策略的最新进展。此外，还讨论了未来优化提高 NMN 产量的前景。

引用次数: 0

Analysis of population heterogeneity in CHO cells by genome-wide DNA methylation analysis and by multi-modal single-cell sequencing 通过全基因组 DNA 甲基化分析和多模式单细胞测序分析 CHO 细胞中的群体异质性。

IF 4.1 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2024-10-31 DOI: 10.1016/j.jbiotec.2024.10.012

Elias Böhl , Günter Raddatz , Suki Roy , Lingzhi Huang , Jasrene Kaur Sandhu , Emeka Ignatius Igwe , Manuel Rodríguez-Paredes , Florian Böhl , Frank Lyko

CHO cells are major hosts for the industrial production of therapeutic proteins and their production stability is of considerable economic significance. It is widely known that CHO cells can rapidly acquire genetic alterations, which affects their genetic homogeneity over time. However, the role of non-genetic mechanisms, including epigenetic mechanisms such as DNA methylation, remains poorly understood. We have now used whole-genome bisulfite sequencing to establish single-base methylation maps of eight independent CHO cell lines. Our results identify CpG islands and low-methylated regions as conserved elements with dynamic DNA methylation. Interestingly, methylation patterns were found to cluster clearly along the three main branches of CHO evolution, with no directional changes over short culture periods. Furthermore, multi-ome single-cell sequencing of 9833 nuclei from three independent cultures revealed dynamic subpopulation structures characterized by robust expression differences in pathways related to protein production. Our findings thus provide novel insights into the epigenetic landscape and heterogeneity of CHO cells and support the development of epigenetic biomarkers that trace the emergence of subpopulations in CHO cultures.

CHO 细胞是治疗蛋白质工业化生产的主要宿主，其生产稳定性具有重要的经济意义。众所周知，CHO 细胞会迅速发生基因改变，从而随着时间的推移影响其基因同质性。然而，人们对非遗传机制（包括 DNA 甲基化等表观遗传机制）的作用仍然知之甚少。现在，我们利用全基因组亚硫酸氢盐测序建立了八个独立 CHO 细胞系的单碱基甲基化图谱。我们的研究结果发现，CpG 岛和低甲基化区域是具有动态 DNA 甲基化的保守元素。有趣的是，我们发现甲基化模式沿着 CHO 进化的三个主要分支明显聚集，在短培养期内没有方向性变化。此外，对来自三个独立培养物的 9,833 个细胞核进行的多组单细胞测序揭示了动态亚群结构，其特点是与蛋白质生产相关的通路存在强大的表达差异。因此，我们的研究结果为了解 CHO 细胞的表观遗传景观和异质性提供了新的视角，并为开发可追踪 CHO 培养物中亚群出现的表观遗传生物标记物提供了支持。

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引用次数: 0

The transcription factor MfbHLH104 from Myrothamnus flabellifolia promotes drought tolerance of Arabidopsis thaliana by enhancing stability of the photosynthesis system 拟南芥转录因子MfbHLH104通过提高光合作用系统的稳定性促进拟南芥的耐旱性。

IF 4.1 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2024-10-29 DOI: 10.1016/j.jbiotec.2024.10.011

Zhuo Huang , Xiangying Xiang , Wenxin Xu , Li Song , Rong Tang , Duoer Chen , Qiao Li , Yujue Zhou , Cai-Zhong Jiang

The resurrection plant Myrothamnus flabellifolia can survive extreme drought and desiccation conditions, and quickly recover after rewatering. However, little is known about the mechanism underlying the drought tolerance of M. flabellifolia. In this study, MfbHLH104 was cloned and introduced into Arabidopsis thaliana due to the lack of a transgenic system for M. flabellifolia. MfbHLH104 is localized in the nucleus. Its N-terminal region has transactivation ability in yeast, and the C-terminal region may inhibit the transactivation ability. Overexpressing MfbHLH104 significantly increased drought and salt tolerance of A. thaliana at both seedling and adult stages. It enhanced leaf water retention capacity by decreasing water loss rate and increasing drought- and abscisic acid (ABA) -induced stomatal closure. Additionally, it boosted osmolyte accumulation and ROS scavenging ability by up-regulating genes associated with osmolyte biosynthesis and antioxidant enzymes, and enhancing antioxidant enzyme activities. The expression of ABA-responsive genes were also promoted by MfbHLH104. Remarkably, RNA-seq analysis indicated that MfbHLH104 significantly up-regulated 32 genes (FDR < 0.05 and fold change ≥1.5) involved in photosynthesis related pathways (KEGG pathway No: ko00195, ko00196) under drought, which account for 18.7 % of the total up-regulated genes and the most enriched KEGG pathways. This result suggested that it may help to maintain the stability of the photosynthesis system under drought conditions.

复活植物花叶蕨（Myrothamnus flabellifolia）能在极端干旱和干燥条件下存活，并在重新浇水后迅速恢复。然而，人们对M. flabellifolia的耐旱机制知之甚少。由于缺乏拟南芥的转基因系统，本研究克隆了 MfbHLH104 并将其导入拟南芥。MfbHLH104 定位于细胞核中。其 N 端区域在酵母中具有反式激活能力，而 C 端区域可能会抑制反式激活能力。过表达 MfbHLH104 能显著提高黄连木幼苗期和成株期的耐旱性和耐盐性。它通过降低失水率和增加干旱和脱落酸（ABA）诱导的气孔关闭来提高叶片的保水能力。此外，它还通过上调与渗透溶质生物合成和抗氧化酶相关的基因以及提高抗氧化酶活性，促进渗透溶质的积累和清除 ROS 的能力。MfbHLH104 还促进了 ABA 响应基因的表达。值得注意的是，RNA-seq分析表明，MfbHLH104显著上调了干旱条件下光合作用相关通路（KEGG通路编号：ko00195、ko00196）的32个基因（FDR<0.05，折合变化率≥1.5），占上调基因总数的18.7%，是KEGG通路中富集最多的通路。这一结果表明，它可能有助于维持干旱条件下光合作用系统的稳定性。

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引用次数: 0

Fractionation of lignin and fermentable sugars from wheat straw using an alkaline hydrogen peroxide/pentanol biphasic pretreatment 使用碱性过氧化氢/戊醇双相预处理法分馏小麦秸秆中的木质素和可发酵糖。

IF 4.1 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2024-10-18 DOI: 10.1016/j.jbiotec.2024.10.004

Yue Wu , Hui Zhang , Qixuan Lin , Ruonan Zhu , Lihong Zhao , Xingjie Wang , Junli Ren , Ling Meng

To breakthrough the delignification saturation point (DSP) of alkaline hydrogen peroxide (AHP) pretreatment, a biphasic AHP/pentanol (AHPP) pretreatment was proposed in this work. The temperature and H₂O₂ concentration were evaluated. Under the optimal conditions (110 °C, 2 h, 4 % H₂O₂), 70.73 % of lignin was removed, which was increased by 11.65 % than the traditional AHP pretreatment, indicating successful overcoming of the DSP by adding pentanol. 85.74 % and 88.62 % of glucan and xylan digestibility were achieved, respectively, which increased by 7.41 % and 5.87 % as compared to AHP pretreatment. Furthermore, the lignin extracted from the organic phase accounted for 38.51 % of the delignification, and it had a low molecular weight, effectively preserving the β-O-4 bonds. Finally, satisfied pentanol recovery (77.91 %) and delignification (57.19 %) along with excellent glucan (76.11 %) and xylan (77.52 %) digestibility were reached after fourth recycling of AHPP pretreatment. Therefore, AHPP pretreatment was a promising method for biomass valorization within biorefinery concept.

为了突破碱性过氧化氢（AHP）预处理的脱木素饱和点（DSP），本研究提出了一种双相 AHP/ 戊醇（AHPP）预处理方法。对温度和 H2O2 浓度进行了评估。在最佳条件下（110 °C、2 小时、4% H2O2），木质素的去除率为 70.73%，比传统的 AHP 预处理提高了 11.65%，表明添加戊醇成功克服了 DSP。葡聚糖和木聚糖的消化率分别达到 85.74% 和 88.62%，比 AHP 预处理提高了 7.41% 和 5.87%。此外，从有机相中提取的木质素占脱木质素的 38.51%，且分子量较低，有效地保留了 β-O-4 键。最后，经过第四次循环 AHPP 预处理后，戊醇回收率（77.91%）和木质素脱除率（57.19%）均达到了令人满意的水平，同时葡聚糖（76.11%）和木聚糖（77.52%）的消化率也很高。因此，在生物精炼概念中，AHPP 预处理是一种很有前景的生物质增值方法。

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引用次数: 0

Lipid analysis of human primary dermal fibroblasts and epidermal keratinocytes after near-infrared exposure using mass spectrometry imaging 利用质谱成像技术分析近红外照射后人原生真皮成纤维细胞和表皮角质细胞的脂质。

IF 4.1 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2024-10-18 DOI: 10.1016/j.jbiotec.2024.10.009

Laura van der Vloet , Zoé Ducarne , Ron M.A. Heeren , Anne C. Berends , Michiel Vandenbosch

Photobiomodulation (PBM) therapy is the application of near-infrared (NIR) exposure to injuries or lesions to (among others) improve wound healing, reduce inflammation, and decreases acute and chronic pain. However, the understanding of the molecular mechanism of PBM, more specifically the effects of NIR on skin cells is still lacking behind. Lipids are essential components of cellular membranes that are integral to skin structure and function. This study aims to elucidate the impact of NIR exposure on the skin’s lipidome by investigating the molecular effect of NIR exposure on single skin cells. Primary human dermal fibroblasts (NHDFa) and human epidermal keratinocytes (HEKa) were exposed to NIR (850 nm) with a dose of 6.5 J/cm² for 5 consecutive days between 09.00 and 12.00 am. A workflow utilizing matrix-assisted laser desorption/ionization mass spectrometry imaging combined with liquid chromatography tandem mass spectrometry for lipidomics analysis was performed. This study provides evidence that adequate exposure of NIR influences lipid metabolism in NHDFa, whereas no alterations were found in HEKa. This work lays the groundwork in explaining the beneficial properties on both skin-related effects and systemic health benefits as seen in clinical studies.

光生物调节（PBM）疗法是将近红外线（NIR）照射到损伤或病变部位，以（除其他外）改善伤口愈合、减轻炎症、减少急性和慢性疼痛。然而，人们对 PBM 的分子机制，特别是近红外对皮肤细胞的影响仍然缺乏了解。脂质是细胞膜的重要组成部分，是皮肤结构和功能不可或缺的组成部分。本研究旨在通过研究近红外照射对单个皮肤细胞的分子影响，阐明近红外照射对皮肤脂质体的影响。原代人类真皮成纤维细胞（NHDFa）和人类表皮角质细胞（HEKa）连续 5 天在上午 09:00 至 12:00 之间暴露于剂量为 6.5J/cm2 的近红外（850nm）。利用基质辅助激光解吸电离质谱成像与液相色谱串联质谱相结合的工作流程进行了脂质组学分析。这项研究提供了证据，证明适当的近红外照射会影响 NHDFa 的脂质代谢，而在 HEKa 中则未发现任何改变。这项工作为解释临床研究中发现的皮肤相关效应和全身健康益处的有益特性奠定了基础。

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引用次数: 0

In-line monitoring of bioreactor by Raman spectroscopy: Direct use of a standard-based model through cell-scattering correction 利用拉曼光谱对生物反应器进行在线监测：通过细胞散射校正直接使用基于标准的模型。

IF 4.1 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2024-10-18 DOI: 10.1016/j.jbiotec.2024.10.007

Ning Yang , Cédric Guerin , Ninel Kokanyan , Patrick Perré

Raman spectroscopy and machine learning have become popular in in-line monitoring of bioreactors. However, traditional modeling processes typically entail extensive fermentation batches to collect learning datasets, which are significantly time–consuming and laborious. In addition, these models are limited to configurations with the same conditions as the training batches. The present work proposes a reproducible and adaptable modeling approach by combining standard spectra as a training dataset, with a simple means of correcting for cell scattering. Alcoholic fermentation by Saccharomyces cerevisiae is used as a benchmark. Initially, a partial least squares (PLS) regression model was developed based on the spectra of pure solutions of glucose and ethanol. Then, a mathematical expression was defined to estimate yeast concentration, allowing the correction of Raman intensity attenuated by cell scattering. The corrected spectra demonstrate close alignment with reference spectra in both shape and intensity. Validation of the methodology was conducted across numerous batches and one fed–batch bioreactor. As a result, the developed method enables the simultaneous monitoring of glucose, ethanol, and yeast concentrations, effectively addressing the challenge of implementing an independent standards based PLS model to manage the intricate compositional dynamics in bio–processes. The conclusion underscores the effectiveness of the proposed method and offers new prospects in biotechnological industries.

拉曼光谱和机器学习已成为生物反应器在线监测的流行方法。然而，传统的建模过程通常需要大量的发酵批次来收集学习数据集，非常耗时耗力。此外，这些模型仅限于与训练批次条件相同的配置。本研究提出了一种可重复、可调整的建模方法，将标准光谱作为训练数据集，并采用简单的细胞散射校正方法。以酿酒酵母的酒精发酵为基准。最初，根据葡萄糖和乙醇纯溶液的光谱建立了偏最小二乘法（PLS）回归模型。然后，定义了一个数学表达式来估算酵母的浓度，从而校正因细胞散射而衰减的拉曼强度。校正后的光谱在形状和强度上都与参考光谱非常接近。在多个批次和一个喂料批次生物反应器中对该方法进行了验证。因此，所开发的方法能够同时监测葡萄糖、乙醇和酵母的浓度，有效地解决了实施基于独立标准的 PLS 模型来管理生物过程中错综复杂的成分动态的难题。结论强调了所建议方法的有效性，并为生物技术产业提供了新的前景。

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引用次数: 0

Investigation into optimizing fermentation processes to enhance uric acid degradation by probiotics 关于优化发酵过程以提高益生菌降解尿酸能力的研究。

IF 4.1 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2024-10-18 DOI: 10.1016/j.jbiotec.2024.10.008

Hanwei Zheng , Zhiqing Liu , Hao Zheng , Yuman Miao , Chenyu Liu , Minhua Zong , Wenyong Lou

To enhance the activity of Lactiplantibacillus plantarum FS4722 (L. plantarum FS4722) in reducing uric acid, this study optimized the culture medium components and fermentation conditions using response surface methodology. The results indicated that the optimal culture medium comprised glucose at 25.61 g/L, yeast extract powder (YEP) at 26.86 g/L, and nucleoside addition at 0.48 g/L. The optimal fermentation conditions were: a fermentation time of 10 h, an initial pH of 6.5, a fermentation temperature of 33 °C, and an inoculum size of 1.0 %. Under these conditions, the nucleoside degradation ability of L. plantarum FS4722 increased by 41.9 times, enhancing its potential applications in reducing uric acid and developing anti-gout foods, health supplements, and pharmaceuticals.

为提高植物乳杆菌 FS4722（L. plantarum FS4722）降低尿酸的活性，本研究采用响应面方法优化了培养基成分和发酵条件。结果表明，最佳培养基包括葡萄糖（25.61 克/升）、酵母提取物粉（26.86 克/升）和核苷（0.48 克/升）。最佳发酵条件是：发酵时间为 10 小时，初始 pH 值为 6.5，发酵温度为 33 °C，接种量为 1.0%。在这些条件下，植物乳杆菌 FS4722 的核苷降解能力提高了 41.9 倍，增强了其在降低尿酸和开发抗痛风食品、保健品和药品方面的潜在应用。

引用次数: 0

Metabolic engineering of Halomonas bluephagenesis for the production of ethylene glycol and glycolate from xylose 从木糖生产乙二醇和乙醇酸盐的蓝光单胞菌代谢工程。

IF 4.1 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2024-10-15 DOI: 10.1016/j.jbiotec.2024.10.006

Yuzhong Liu , Kai Huo , Biwei Tan , Xulin He , Qiong Wu , Zheng-Jun Li

Halophilic Halomonas bluephagenesis, a natural producer of poly-3-hydroxybutyrate (PHB), was metabolically engineered to synthesize ethylene glycol and glycolate from xylose. Xylose utilization was achieved by overexpressing either the xylonate pathway or the ribulose-1-phosphate pathway. The key genes encoding for xylonate dehydratase and 2-keto-3-deoxy-xylonate aldolase in the xylonate pathway were screened. With further overexpressing aldehyde reductase gene yjgB, ethylene glycol accumulation was improved to 0.91 g/L, accompanied with 1.48 g/L of PHB accumulation. The disruption of native glycolate oxidase was found to be essential for glycolate production, and the defective recombinant strain produced 0.80 g/L glycolate with 1.14 g/L PHB in shake flask cultures. These results indicated that H. bluephagenesis has the potential to produce diverse metabolic chemicals from xylose.

嗜卤卤单胞菌（Halomonas bluephagenesis）是聚-3-羟基丁酸（PHB）的天然生产者，经代谢改造后，可从木糖合成乙二醇和乙醇酸。木糖的利用是通过过量表达木糖酸途径或核酮糖-1-磷酸途径来实现的。筛选出了木糖途径中编码木糖脱水酶和 2-酮-3-脱氧木糖醛缩酶的关键基因。通过进一步过表达醛还原酶基因 yjgB，乙二醇积累量提高到 0.91 克/升，同时 PHB 积累量达到 1.48 克/升。在摇瓶培养中，有缺陷的重组菌株产生了 0.80g/L 乙二醇和 1.14g/L PHB。这些结果表明，H. bluephagenesis 具有利用木糖生产多种代谢化学品的潜力。

引用次数: 0

Recycling potential of Cupriavidus necator for life support in space: Production of SCPs from volatile fatty acid and urea mixture 用于太空生命支持的Cupriavidus necator的回收潜力：利用挥发性脂肪酸和尿素混合物生产 SCPs

IF 4.1 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2024-10-12 DOI: 10.1016/j.jbiotec.2024.10.001

P. Joris , E. Lombard , A. Paillet , G. Navarro , S.E. Guillouet , N. Gorret

The International Space Station currently requires four annual replenishments for food supply, a practice that won't be feasible for deep space missions due to the greater distances. Based on the design of closed ecological life support systems, two waste streams were identified: urea from the crew urine, volatile fatty acids (VFAs) from a first stage of anaerobic digestion of waste. The objective of this study was to assess the ability of bacterium Cupriavidus necator to produce single cell protein on urea and VFAs. Thus, the effect of carbon sources (glucose vs VFAs) and the dilution rate on the biomass composition was determined in continuous cultures. Complete transformation of the carbon source into protein-rich biomass was achieved up to 78 % cell dry weight (CDW). For both carbon sources, the protein content increased from 55.0 %CDW to 78 %CDW with a decrease in the dilution rate. Conversely, the nucleic acid and polyhydroxyalkanoate contents decreased with the dilution rate from 8.8 %CDW to 4.8 %CDW and 9.8 %CDW to 0.6 %CDW respectively. Working at a low dilution rate seems to be a good way to maximize protein content while minimizing unwanted nucleic acids and polyhydroxyalkanoates.

国际空间站目前需要每年补充四次食物，由于距离较远，这种做法在深空任务中并不可行。根据封闭式生态生命支持系统的设计，确定了两种废物流：来自乘员尿液的尿素和来自废物厌氧消化第一阶段的挥发性脂肪酸（VFAs）。本研究的目的是评估坏死杯状芽孢杆菌（Cupriavidus necator）利用尿素和挥发性脂肪酸产生单细胞蛋白质的能力。因此，在连续培养中确定了碳源（葡萄糖与 VFAs）和稀释率对生物质组成的影响。碳源完全转化为富含蛋白质的生物质的细胞干重（CDW）达到 78%。对于两种碳源，随着稀释率的降低，蛋白质含量从 55.0 %CDW 增加到 78 %CDW。相反，核酸和多羟基烷酸含量则随着稀释率的降低而减少，分别从 8.8 %CDW 降至 4.8 %CDW 和 9.8 %CDW 降至 0.6 %CDW。采用低稀释率似乎是一种很好的方法，既能最大限度地提高蛋白质含量，又能最大限度地减少不需要的核酸和多羟基烷酸。

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引用次数: 0