Journal of biotechnology最新文献_第2页

Multilevel agro-physiological and biochemical alleviation of salt stress in Olea europaea via phenolic-rich extracts from olive mill waste 利用橄榄磨废料中富含酚类物质的提取物，多级缓解油橄榄盐胁迫的农业生理生化作用

IF 3.9 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2026-01-27 DOI: 10.1016/j.jbiotec.2026.01.013

Samia Abboud , Nada Ammar , Azhar Ouni , Mourad Jellali , Darine Tlili , Sahar Ben Abdelwaheb , Amani Bchir , Soumaya Dbara

Soil salinization poses a critical threat to agricultural productivity, particularly in the Mediterranean regions where olive cultivation is of major economic and ecological significance. This study investigates the potential of phenolic extracts derived from olive mill solid waste (OMSW) to enhance salt stress tolerance in Olea europaea L. cv. Koroneiki. One-year-old olive plants were subjected to six treatments: a non-stressed control (C), phenolic extract application under non-stress conditions (C + PE-OMSW), OMSW amendment under non-stress conditions (C + OMSW), salt stress induced by 100 mM NaCl (SS), salt stress combined with phenolic extract (SS + PE-OMSW), and salt stress combined with OMSW (SS + OMSW). A comprehensive physiological and biochemical evaluation was conducted, including measurements of vegetative growth, relative water content, membrane stability, chlorophyll fluorescence, photosynthetic pigments, oxidative stress markers (malondialdehyde and hydrogen peroxide), osmoprotectants (proline and soluble sugars), total polyphenols, flavonoids, and leaf mineral content. Application of phenolic extract under saline conditions (SS + PE-OMSW) markedly improved plant performance by enhancing water status, preserving membrane integrity, and increasing chlorophyll fluorescence efficiency. These plants also exhibited higher polyphenols and flavonoids accumulation, along with significant reductions in oxidative stress markers, suggesting enhanced antioxidant defenses. Elevated levels of proline and soluble sugars further indicated an adaptive osmotic adjustment to salinity. These results demonstrate the efficacy of OMSW-derived phenolic extracts as sustainable biostimulants capable of mitigating salt stress through integrated physiological and biochemical mechanisms. This valorization pathway offers a promising approach to recycling agro-industrial residues into high-value agricultural inputs, contributing to climate-resilient and circular bioeconomy-based crop production systems.

土壤盐碱化对农业生产力构成严重威胁，特别是在橄榄树种植具有重大经济和生态意义的地中海地区。本研究探讨了橄榄厂固体废物（OMSW）酚类提取物提高油橄榄（Olea europaea L. cv）耐盐性的潜力。Koroneiki。对1年生橄榄植株进行6个处理：无胁迫对照(C)、无胁迫条件下施用酚类提取物（C + PE-OMSW）、无胁迫条件下添加OMSW （C + OMSW）、100 mM NaCl诱导盐胁迫（SS）、盐胁迫+酚类提取物（SS + PE-OMSW）、盐胁迫+ OMSW （SS + OMSW）。进行了全面的生理生化评价，包括营养生长、相对含水量、膜稳定性、叶绿素荧光、光合色素、氧化应激标志物（丙二醛和过氧化氢）、渗透保护剂（脯氨酸和可溶性糖）、总多酚、类黄酮和叶片矿物质含量的测定。在生理盐水条件下施用酚提取物（SS + PE-OMSW），通过改善水分状态、保持膜完整性和提高叶绿素荧光效率，显著改善了植物的生产性能。这些植物还表现出更高的多酚和类黄酮积累，以及氧化应激标志物的显著减少，表明抗氧化防御能力增强。脯氨酸和可溶性糖水平的升高进一步表明了对盐度的适应性渗透调节。这些结果表明，omsw衍生的酚类提取物作为一种可持续的生物刺激剂，能够通过综合的生理和生化机制缓解盐胁迫。这一增值途径为将农业工业残留物循环利用为高价值农业投入物提供了一种有希望的方法，有助于建立适应气候变化和基于循环生物经济的作物生产系统。

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引用次数: 0

Bispecific nanobodies – promising engineered candidates with high therapeutic efficiency 双特异性纳米体-具有高治疗效率的有前途的工程候选物。

IF 3.9 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2026-01-25 DOI: 10.1016/j.jbiotec.2026.01.010

Sneha Banerjee, Sreeja Vangapally, Anna Mariya, Bhaskar Paidimuddala

Bispecific nanobodies are the emerging class of engineered nanobodies with great scope for targeted therapy due to their small size, high stability, extended half-life, and dual antigenic recognition properties. They can bind to two different antigens simultaneously which significantly improves the therapeutic index in the treatment of various diseases. To achieve enhanced binding avidity, specificity and minimal off-target effects, the bispecific nanobodies have been further subjected to advanced engineering strategies such as site-specific combinations, fusions and multivalent formats. The recombinant production of bispecific nanobodies has been tested in bacterial, yeast and mammalian expression platforms to facilitate their large-scale production and cost-effective clinical applications. This review exclusively focuses on recent progress in the design and development of bispecific nanobodies. It presents the engineering of nanobodies into bispecific formats, their design, expression strategies and therapeutic outcomes. It also discusses the recent preclinical and clinical developments of bispecific nanobodies focusing on tackling half-life extension problems, reducing immunogenicity and optimal delivery modalities. Overall, with the promising scope of bispecific nanobodies as targeted therapies, their acceptance into mainstream medicine holds great promise for precision and personalized therapeutic approaches for the effective treatment of various infections, cancers, autoimmune and neurodegenerative diseases.

双特异性纳米体是一类新兴的工程纳米体，由于其体积小、稳定性高、半衰期长和双重抗原识别特性，具有很大的靶向治疗范围。它们可以同时结合两种不同的抗原，显著提高了治疗各种疾病的治疗指标。为了实现更强的结合亲和性、特异性和最小的脱靶效应，双特异性纳米体进一步受到先进的工程策略的影响，如位点特异性组合、融合和多价格式。重组生产双特异性纳米体已经在细菌、酵母和哺乳动物表达平台上进行了测试，以促进其大规模生产和具有成本效益的临床应用。本文综述了双特异性纳米体的设计和开发的最新进展。它介绍了纳米体工程成双特异性格式及其设计和表达策略和治疗结果。它还讨论了最近双特异性纳米体的临床前和临床发展，重点是解决半衰期延长问题，降低免疫原性和最佳递送方式。总的来说，随着双特异性纳米体作为靶向治疗的前景广阔，它们被主流医学接受，为有效治疗各种感染、癌症、自身免疫和神经退行性疾病的精确和个性化治疗方法带来了巨大的希望。

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引用次数: 0

Dynamic regulation of ROS stress in the accumulation of L-lysine in Escherichia coli 活性氧胁迫对大肠杆菌赖氨酸积累的动态调控。

IF 3.9 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2026-01-24 DOI: 10.1016/j.jbiotec.2026.01.011

Li Zhang, Jianan Yang, Zhixuan Lv, Longjun Han, Zhiwei Zha, Zhijie Cheng, Xu Yang, Zhengyang Xu, Lei Yang, Jian Gao

Oxidative stress in Escherichia coli during chemical production, caused by reactive oxygen species (ROS), impairs cell viability and limits output. This study addressed product accumulation and ROS stress in L-lysine-producing strains. Genetic engineering created three variants: dynamically up-regulated R1, down-regulated R2, and temporally regulatable R3 to reduce ROS during fermentation. Under stress conditions, all engineered strains showed significantly lower ROS levels versus parental strain R0, with R3 exhibiting the greatest reduction. Fermentation confirmed R3's superior performance, yielding 85.8 % more L-lysine than R0. These results demonstrate that constructing ROS mitigator achieves precise ROS control and efficient L-lysine synthesis. This dynamic regulation strategy enhances cell viability and production performance under oxidative stress, providing a viable approach for improving engineered cell factories' stress resistance.

在化学生产过程中，由活性氧（ROS）引起的大肠杆菌氧化应激会损害细胞活力并限制产量。本研究研究了l -赖氨酸产生菌株的产物积累和ROS胁迫。基因工程创造了三种变体：动态上调的R1，下调的R2和暂时可调节的R3，以减少发酵过程中的ROS。在胁迫条件下，与亲本菌株R0相比，所有工程菌株的ROS水平均显著降低，其中R3的降幅最大。发酵证实了R3的优越性能，其l -赖氨酸产量比R0高出85.8%。这些结果表明，构建ROS缓解剂可以实现ROS的精确控制和l -赖氨酸的高效合成。这种动态调控策略提高了氧化胁迫下的细胞活力和生产性能，为提高工程细胞工厂的抗逆性提供了可行的途径。

引用次数: 0

Streptomyces bottropensis SY8-derived eumelanin exhibits skin wound healing activity and prevents H2O2 or UV-induced skin aging 肉毒杆菌链霉菌sy8衍生的真黑色素显示皮肤伤口愈合活性，并防止H2O2或紫外线诱导的皮肤老化

IF 3.9 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2026-01-21 DOI: 10.1016/j.jbiotec.2026.01.009

Sevval Yildirim , Buket Bakan , Meryem Doymus , Seydanur Elmas , Nazli Pinar Arslan , Mesut Taskin

Streptomyces-derived melanins exhibit diverse bioactive properties. This study aims to (1) produce melanin production from Streptomyces bottropensis SY8 (GenBank accesion number: PQ565816), (2) characterize the melanin, and (3) investigate its bioactive properties (toxicity and irritancy as well as invitro wound healing and anti-skin aging activities). Under optimized culture conditions, melanin production of 3.26 g/L was achieved in shaking flask cultures of the bacterium. The purified melanin was characterized as eumelanin. Compared to the control (Vitamin C), the purified melanin was found to have moderate antioxidant activity in radical scavenging assays. It did not cause toxicity on fibroblast cell line and irritancy in HET-CAM test (hen's egg test on chorioallantoic membrane test). The melanin reduced reactive oxygen species (ROS) accumulation and cell senescence induced by H₂O₂ or UV in fibroblast cells, indicating its anti-skin aging potential. When compared the control (melanin free), the melanin doses of 500 µg/mL and 1000 µg/mL caused increments of 17.59 % and 24.53 % in wound closure ratios at the end of the 24th hour, respectively. This is the first report on melanin production from S. bottropensis. Besides, in vitro wound healing and anti-skin aging activities of Streptomyces-derived melanins were investigated for the first time. Furthermore, HET-CAM test was used for the first time to analyze irritancy property of melanins. The results of this study indicate that S. bottropensis SY8-derived eumelanin can be exploited as an ingredient of anti-aging creams or wound dressings.

链霉菌衍生的黑色素具有多种生物活性。本研究旨在(1)产自肉毒杆菌链霉菌SY8 (GenBank加入编号：PQ565816),(2)表征黑色素，(3)研究其生物活性特性（毒性、刺激性、体外伤口愈合和抗皮肤衰老活性）。在优化的培养条件下，细菌摇瓶培养的黑色素产量为3.26 g/L。纯化后的黑色素被定性为真黑色素。与对照（维生素C）相比，在自由基清除试验中发现纯化黑色素具有中等的抗氧化活性。对成纤维细胞系无毒性作用，对绒毛膜-尿囊膜试验（HET-CAM）无刺激性。黑色素可减少成纤维细胞中活性氧（ROS）的积累和H2O2或UV诱导的细胞衰老，显示其抗皮肤衰老的潜力。与对照组（无黑色素）相比，500 µg/mL和1000 µg/mL的黑色素剂量在24小时结束时分别使伤口闭合率增加17.59 %和24.53 %。这是关于肉毒杆菌产生黑色素的第一篇报道。此外，我们还首次研究了链霉菌黑色素的体外创面愈合和抗皮肤衰老活性。首次采用ht - cam检测方法分析黑色素的刺激性。本研究结果表明，肉毒杆菌sy8衍生的真黑素可以作为抗衰老乳膏或伤口敷料的成分。

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引用次数: 0

Antibacterial nut grass cellulose reinforced polylactic acid nanocomposites: A holistic assessment for biomedical scaffolds 抗菌坚果草纤维素增强聚乳酸纳米复合材料：生物医学支架的整体评价。

IF 3.9 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2026-01-21 DOI: 10.1016/j.jbiotec.2026.01.008

Priya S.A., Nevaditha N.T.

Poly(lactic acid) (PLA) composites reinforced with various natural fibers have been extensively investigated for biomedical applications. However, the use of nut grass-derived cellulose nanofibers (CNFs) remains unexplored in the current literature. This study presents the development of PLA nanocomposites reinforced with CNFs extracted from nut grass for biomedical applications. The PLA matrix was chemically modified using ethylene glycol dimethacrylate (EGDMA) and plasticized with castor oil. SEM analysis revealed uniform dispersion of CNFs at lower concentrations, leading to improved film morphology. XRD analysis indicated a CNF particle size of approximately 26 nm. Mechanical testing showed significant enhancements in material strength, with tensile strength and Young’s modulus increasing by 97.1 % and 113.9 %, respectively. The nanocomposites showed greater degradation in alkaline than acidic medium due to accelerated hydrolysis. Antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa increased with CNF content. Hemolysis assays revealed reduced red blood cell damage compared to plain PLA, indicating favorable hemocompatibility. Additionally, MTT assay confirmed low cytotoxicity of the nanocomposites toward PBMC cells. These results demonstrate the potential of PLA nanocomposites as biocompatible, multifunctional materials suitable for applications in implants, wound healing and regenerative medicine.

以多种天然纤维为增强材料的聚乳酸（PLA）复合材料在生物医学领域的应用得到了广泛的研究。然而，使用坚果草衍生的纤维素纳米纤维（CNFs）在目前的文献中仍未被探索。本文介绍了以坚果草为原料提取CNFs增强聚乳酸纳米复合材料的研究进展。用乙二醇二甲基丙烯酸酯（EGDMA）对聚乳酸（PLA）基体进行化学改性，并用蓖麻油进行增塑。扫描电镜分析显示，低浓度CNFs分散均匀，导致膜形态改善。XRD分析表明CNF粒径约为26nm。力学试验表明，材料强度显著提高，抗拉强度和杨氏模量分别提高了97.1%和113.9%。由于水解加速，纳米复合材料在碱性介质中比在酸性介质中更容易降解。对金黄色葡萄球菌和铜绿假单胞菌的抑菌活性随CNF含量的增加而增加。溶血试验显示，与普通聚乳酸相比，红细胞损伤减少，表明良好的血液相容性。此外，MTT实验证实了纳米复合材料对PBMC细胞的低细胞毒性。这些结果证明了聚乳酸纳米复合材料作为生物相容性、多功能材料的潜力，适合应用于植入物、伤口愈合和再生医学。

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引用次数: 0

Integrative omics approaches for bioactive metabolite discovery in marine macroalgae: Recent advances and future perspectives 综合组学方法在海洋大型藻类生物活性代谢物的发现：最新进展和未来展望。

IF 3.9 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2026-01-19 DOI: 10.1016/j.jbiotec.2026.01.007

M. Marimuthu , M. Anitha , P. Prakash , Meivelu Moovendhan

Phlorotannins, bromophenols, sulfated polysaccharides, terpenoids, lipids and halogenated molecules exhibit potent antioxidant, anticancer, anti-inflammatory, and antimicrobial properties. Conventional discovery methods, such as solvent extraction and bioassay-guided fractionation, are limited by low throughput, specificity, and poor insight into biosynthetic mechanisms. High-throughput omics platforms including genomics, transcriptomics, proteomics, and metabolomics have transformed the discovery of bioactive metabolites in macroalgae by unraveling system-wide interpretation of biosynthetic pathways. This review outlines omics-driven strategies for macroalgal natural product research, highlighting genome sequencing and annotation efforts. Transcriptome-based biosynthetic gene cluster (BGC) mining, and gene regulation analysis via RNA-seq. Proteomic approaches such as 2D electrophoresis, LC-MS/MS, and MALDI-TOF are discussed for their role in elucidating enzyme functions and post-translational modifications. Metabolomics tools, including GC-MS, LC-MS, and NMR, paired with platforms like GNPS and MetaboAnalyst, have improved metabolite identification, dereplication and pathway analysis. Emerging integrative multi-omics frameworks combining machine learning and systems biology are paving the way for predictive, precision-driven bioprospecting. Nonetheless, challenges persist, including incomplete genome assemblies, taxonomic inconsistencies and limited reference databases. This review emphasizes how omics technologies are deciphering and mechanistically elucidating the untapped metabolic potential of marine macroalgae, accelerating their application in biotechnology and therapeutic innovation.

绿单宁、溴酚、磺化多糖、萜类、脂类和卤化分子表现出强大的抗氧化、抗癌、抗炎和抗菌特性。传统的发现方法，如溶剂萃取和生物测定引导分离，受到低通量、特异性和对生物合成机制缺乏了解的限制。包括基因组学、转录组学、蛋白质组学和代谢组学在内的高通量组学平台通过揭示生物合成途径的全系统解释，改变了大型藻类中生物活性代谢物的发现。本文概述了大藻天然产物研究的组学驱动策略，重点介绍了基因组测序和注释工作。基于转录组的生物合成基因簇（BGC）挖掘及RNA-seq基因调控分析。蛋白质组学方法，如二维电泳，LC-MS/MS和MALDI-TOF讨论了它们在阐明酶功能和翻译后修饰中的作用。代谢组学工具，包括GC-MS， LC-MS和NMR，与GNPS和MetaboAnalyst等平台配对，改进了代谢物鉴定，去复制和途径分析。结合机器学习和系统生物学的新兴综合多组学框架为预测、精确驱动的生物勘探铺平了道路。然而，挑战仍然存在，包括不完整的基因组组装，分类不一致和有限的参考数据库。本文着重介绍了组学技术如何破译和机械地阐明海洋巨藻未开发的代谢潜力，加速其在生物技术和治疗创新中的应用。

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引用次数: 0

Integrative experimental and computational approach for the development of novel plasminogen activators from truncated streptokinase fragments 从截断的链激酶片段中开发新型纤溶酶原激活剂的综合实验和计算方法。

IF 3.9 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2026-01-16 DOI: 10.1016/j.jbiotec.2026.01.004

Vijay Gunasekaran , Suthakaran Pichaimuthu , Vigneshwaran Namasivayam , V. Ponnusami

Streptokinase (SK), a widely used thrombolytic agent, activates human plasminogen, which in turn dissolves blood clots. However, its clinical application is limited because it elicits immunogenic responses in patients. The present study aims to develop novel plasminogen activators derived from truncated fragments of SK that retain activity comparable to the full-length SK enzyme. Based on the structure-function relationships and the previous literature, ten fragments of SK were designed by systematically truncating the N- and C-terminal regions and the central β-domain. These fragments included variants lacking N-terminal residues (amino acids 1–24 or 1–37), C-terminal truncations (beyond amino acid 300) and segments focused on the central domain (starting at amino acid 130 or ending at 173). Fragments were expressed in E. coli and their plasminogen activation activity was evaluated. The experimental results indicated that fragments containing an intact N1 domain retained a high level of plasminogen activation activity (fragments 1–300, 1–173), while deletion of key N-terminal residues significantly reduced it. C-terminal fragments partially compensated for N-terminal loss (fragment 130–414), but overall activity remained lower. Molecular modelling studies of protein–protein complex interface analyses revealed that the N1 domain (residues 1–173) of SK constitutes the principal binding interface with HPlg, with specific N-terminal residues serving as interaction hotspots. Residue-level contact mapping correlated strongly with experimental activity, underscoring the importance of precise interface residues rather than overall domain-level contact coverage. The study identifies SK fragments 1–300, 1–173 and 130–414 as having substantial activity comparable to full-length SK, suggesting that these constructs could serve as the basis for novel thrombolytic drug candidates pending further immunogenicity evaluation. These interesting findings provide both structural and functional insights for the rational design of improved streptokinase-derived therapeutics.

链激酶（SK），一种广泛使用的溶栓剂，激活人纤溶酶原，进而溶解血凝块。然而，它的临床应用受到限制，因为它会引起患者的免疫原性反应。目前的研究旨在开发新的纤溶酶原激活剂，从SK的截短片段中提取，保留与全长SK酶相当的活性。基于结构-功能关系和已有文献，通过系统截断N端和c端区域以及中心β域，设计了10个SK片段。这些片段包括缺乏n端残基（氨基酸1-24或1-37），c端截断（超过氨基酸300）和集中在中心结构域的片段（从氨基酸130开始或结束于173）的变体。片段在大肠杆菌中表达，并对其纤溶酶原活化活性进行了评价。实验结果表明，含有完整N1结构域的片段（片段1- 300,1 -173）保留了高水平的纤溶酶原激活活性，而关键n端残基的缺失显著降低了其活性。c端片段部分补偿了n端损失（片段130-414），但总体活性仍然较低。蛋白质-蛋白质复合物界面分析的分子模型研究表明，SK的N1结构域（残基1-173）是与HPlg的主要结合界面，特定的n端残基是相互作用的热点。残留物水平的接触映射与实验活动密切相关，强调了精确的界面残留物的重要性，而不是整个领域水平的接触覆盖。该研究确定SK片段1-300、1-173和130-414具有与全长SK相当的实质性活性，这表明这些结构可以作为新型溶栓药物候选药物的基础，有待进一步的免疫原性评估。这些有趣的发现为合理设计改进的链激酶衍生疗法提供了结构和功能上的见解。

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引用次数: 0

Couple action of some proteases from feather keratin-degrading bacterium B.licheniformis CP-16 羽毛角蛋白降解细菌B.licheniformis CP-16部分蛋白酶的偶联作用。

IF 3.9 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2026-01-14 DOI: 10.1016/j.jbiotec.2026.01.006

Shijin Luo , Deshan Wang , Kaige Yang , Hui Ye , Yingxia Sun , Zhiguo Qi , Jianjun Zuo , Tieying Zhang

A keratin-degrading bacterium, Bacillus licheniformis CP-16 (B.licheniformis CP-16), isolated from feather waste, was found to produce three extracellular proteins with protease activity. Following mass spectrometry sequencing and BLAST analysis, these proteins exhibited high similarity to γ-glutamyl transpeptidase, alkaline serine protease and thioredoxin-like protein YkuU, respectively. Genes of the three proteins and Ker A from B.licheniformis were successfully cloned into pET22b and expressed in Escherichia coli (E coli), and the four recombinant enzymes were named P-Glu, P-Alk, P-Trx, and P-Ker. P-ker showed great keratinase activity (4.9 kU/mg) and casein protease activity (6.5 kU/mg), as P-Alk showed keratinase activity (1.2 kU/mg) and casein protease activity (23 kU/mg). In contrast, both P-Glu and P-Trx displayed low levels of keratinase and casein protease activities. P-Trx had disulfide bond reductase activity (3 U/mg). When mixing with P-Trx, the other three proteases, P-Glu, P-Alk, P-Ker degraded feather keratin with keratinase activity promotion of 71 %, 40 % and 71 %. Commercial proteases (trypsin and chymotrypsin) also got keratinolytic capacity after mixing with P-Trx. These findings reveal a novel synergistic mechanism between P-Trx and other proteases in the degradation of feather keratin, leading to more efficient keratin breakdown.

从羽毛废弃物中分离到一种角蛋白降解细菌地衣芽孢杆菌CP-16 (B.licheniformis CP-16)，发现其可产生3种具有蛋白酶活性的细胞外蛋白。通过质谱测序和BLAST分析，这些蛋白分别与γ-谷氨酰转肽酶、碱性丝氨酸蛋白酶和硫氧还蛋白样蛋白YkuU具有高度的相似性。将地衣原体中3种蛋白和Ker A基因成功克隆到pET22b中，并在大肠杆菌中表达，4种重组酶分别命名为P-Glu、P-Alk、P-Trx和P-Ker。P-ker具有较高的角化酶活性（4.9 kU/mg）和酪蛋白蛋白酶活性（6.5 kU/mg）， P-Alk具有较高的角化酶活性（1.2 kU/mg）和酪蛋白蛋白酶活性（23 kU/mg）。相反，P-Glu和P-Trx的角化酶和酪蛋白蛋白酶活性均较低。P-Trx具有二硫键还原酶活性（3u /mg）。与P-Trx混合后，P-Glu、P-Alk、P-Ker降解羽毛角蛋白，角蛋白酶活性分别提高71%、40%和71%。商业蛋白酶（胰蛋白酶和凝乳胰蛋白酶）与P-Trx混合后也具有角化能力。这些发现揭示了P-Trx和其他蛋白酶在羽毛角蛋白降解中的一种新的协同机制，从而导致更有效的角蛋白分解。

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引用次数: 0

Marine-derived Streptomyces sp. isolated from the Persian Gulf as a novel source of melanin 从波斯湾分离的海产链霉菌属，作为黑色素的新来源。

IF 3.9 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2026-01-14 DOI: 10.1016/j.jbiotec.2026.01.005

Seyed Muhammad Hamid Malekpour , Moj Khaleghi , Alireza Akhtarpoor , Mostafa Pournamdari , Hoda KeshmiriNeghab , Farideh MohammadHosseinZadeh

In this study, the structure and biological properties of melanin pigment produced by Streptomyces strain SEA5 isolated from Persian Gulf sediments were investigated. This strain was cultured in an enriched culture medium (L-tyrosine, meat extract). Pigment production was also performed in culture media containing different sources of amino acid L-tyrosine such as soybean meal. Pigment extraction was performed using hydrochloric acid (6 M). In order to investigate its physicochemical nature, TLC, HPLC, UV-Vis spectroscopy, FTIR and 1 H NMR analysis was performed. On the other hand, strong UV absorption with SPF, antioxidant activity and cytotoxicity assay of this pigment were also studied. The results showed that strain SEA5 was well grown and melanized well (1.5 g/L) in medium containing L-tyrosine. It was 1.36 g/L when cultured in medium containing soybean meal. The UV–vis spectrum had an absorption peak at 225 nm. FTIR identified the functional groups of eumelanin with very high peaks at 3414.46 cm-1 (N-H group) and 1650–1540 cm-1 (CO and CN vibrations). Also, the ¹HNMR spectrum showed several peaks between 6.9 and 8.0 ppm, indicating aromatic protons. On the other hand, the extracted pigment had an SPF of 22.55 and showed significant antioxidant activity with 60–77 % reduction of DPPH radicals. The pigment in the formulation selectively inhibited the growth of A375 melanoma cells (IC50- 79.82 μg/mL), whilst it was non-toxic when tested against normal human dermal fibroblasts and HaCaT keratinocytes. Overall, these findings suggest the potential of SEA5-derived melanin as a human skin care product, pharmaceutical product and antioxidant material.

本研究对波斯湾沉积物中分离的链霉菌SEA5产黑色素的结构和生物学特性进行了研究。该菌株在富集培养基（l -酪氨酸，肉提取物）中培养。在含有不同来源的l -酪氨酸（如豆粕）的培养基中也进行了色素生产。用盐酸（6M）提取色素。为考察其理化性质，采用TLC、HPLC、UV-Vis、FTIR和1H NMR分析。另一方面，对该色素的强紫外吸收、抗氧化活性和细胞毒性进行了研究。结果表明，菌株SEA5在含有L-酪氨酸的培养基中生长良好，黑色素化良好（1.5g/L）。在含有豆粕的培养基中培养时为1.36g/L。紫外可见光谱在225nm处有一个吸收峰。FTIR鉴定了真黑素的官能团，在3414.46 cm-1 （N- h基团）和1650-1540 cm-1 （C=O和C=N振动）处有很高的峰。1HNMR谱在6.9 ~ 8.0 ppm之间有几个峰，表明存在芳香质子。另一方面，提取的色素的SPF值为22.55，具有显著的抗氧化活性，DPPH自由基降低60-77%。该配方中的色素选择性抑制A375黑色素瘤细胞的生长（IC50- 79.82μg/ml），而对正常人真皮成纤维细胞和HaCaT角质形成细胞无毒。总之，这些发现表明sea5衍生黑色素作为人类护肤品、医药产品和抗氧化材料的潜力。

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引用次数: 0

Corrigendum to “Structure–function analysis and exonuclease deletion yield an improved strand-displacing DNA polymerase from Aeribacillus pallidus for efficient recombinase polymerase amplification” [J. Biotechnol. 410 (2026) 321–330] “结构-功能分析和外切酶缺失产生改进的白芽孢杆菌置换链DNA聚合酶，用于高效扩增重组酶聚合酶”的更正[J]。生物工程学报，2010 (6):391 - 391 [j]

IF 3.9 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology

Pub Date : 2026-01-14 DOI: 10.1016/j.jbiotec.2026.01.003

Koki Nishi , Eisuke Inoue , Yuto Murakami , Yuri Ishii , Yukiko Nakura , Itaru Yanagihara , Kiyoshi Yasukawa , Shinsuke Fujiwara

引用次数: 0