Pub Date : 2021-09-15DOI: 10.21608/jbaar.2021.195281
N. Almokhtar, N. Ali
Background: Most blood collection bags contain 63 mL CPDA anticoagulant which is sufficient to anticoagulant and ensures the viability of blood cells in 450 mL±10% blood for up to 28–35 days when the blood is stored at 2–8°C. Prolonged storage of blood leads to alteration in cells hematologically which may lose viability with time. Aim : The study was conducted to determine the effect of storage on CPDA-1 for varying periods on some hematological parameters. Materials and methods: The study was conducted on blood donated by 30 healthy volunteer donors. Effect of storage was analyzed at 1, 7, 14, 21and 28 days intervals. Hematological parameters were measured using Mindary PS 300 hematology analyzer. Results: There is a highly significant increase in hemoglobin concentration, packed cell volume (P.C.V. %), MCV, and also a decrease in lymphocyte, granulocyte, and platelet count. The results also showed an insignificant decrease in total white blood cell count. Conclusion: There are degenerative changes observed in blood parameters in samples collected in citrate phosphate dextrose adenine (CPDA-1).
背景:大多数采血袋含有63 mL CPDA抗凝剂，在450 mL±10%的血液中，当血液在2-8°C保存时，CPDA抗凝剂足以抗凝并保证血细胞的活力长达28-35天。血液的长期储存导致细胞血液学上的改变，可能随着时间的推移而失去活力。目的:研究不同贮存时间CPDA-1对血液学指标的影响。材料与方法:研究对象为30名健康志愿献血者。每隔1、7、14、21、28天分析贮藏效果。采用迈达PS 300血液学分析仪测定血液学参数。结果:血红蛋白浓度、堆积细胞体积(pcv %)、MCV显著升高，淋巴细胞、粒细胞和血小板计数降低。结果还显示，总白细胞计数明显下降。结论:枸橼酸磷酸葡萄糖腺嘌呤(CPDA-1)血液参数有退行性改变。
{"title":"CDPA-1 Stored Blood Induced Effect on some Haematological Parameters","authors":"N. Almokhtar, N. Ali","doi":"10.21608/jbaar.2021.195281","DOIUrl":"https://doi.org/10.21608/jbaar.2021.195281","url":null,"abstract":"Background: Most blood collection bags contain 63 mL CPDA anticoagulant which is sufficient to anticoagulant and ensures the viability of blood cells in 450 mL±10% blood for up to 28–35 days when the blood is stored at 2–8°C. Prolonged storage of blood leads to alteration in cells hematologically which may lose viability with time. Aim : The study was conducted to determine the effect of storage on CPDA-1 for varying periods on some hematological parameters. Materials and methods: The study was conducted on blood donated by 30 healthy volunteer donors. Effect of storage was analyzed at 1, 7, 14, 21and 28 days intervals. Hematological parameters were measured using Mindary PS 300 hematology analyzer. Results: There is a highly significant increase in hemoglobin concentration, packed cell volume (P.C.V. %), MCV, and also a decrease in lymphocyte, granulocyte, and platelet count. The results also showed an insignificant decrease in total white blood cell count. Conclusion: There are degenerative changes observed in blood parameters in samples collected in citrate phosphate dextrose adenine (CPDA-1).","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86740744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-01DOI: 10.21608/jbaar.2021.178505
A. Zaid, S. Merdas, Muntaha Y. Hayal
Corrosion is the most important and costly cause of severe operational problems encountered in oil and gas production systems. It may occur anywhere in the production system, from the oil/gas well bottom to the final transfer of produced gas or oil to the refinery. Internal corrosion of carbon steel pipelines is a common and serious problem in oil and gas production, which is designed for long-term operation. It involves an interaction between the metal wall and the flowing fluids. This problem has caused the consideration of many corrosion control programs and research in various oilfields around the world. There is global use of carbon steels as materials of construction for pipes in the oil and gas industries. Compounds triazoles and their derivatives have attracted huge attention due to their simple preparation and superior corrosion inhibition activity towards mild steel in acidic environments. Therefore, the present review aims to highlight the carried out using Compounds triazoles and their derivatives for steel protection in different corrosive environments.
{"title":"Heterocyclic Compounds Containing N atoms as Corrosion Inhibitors: A review","authors":"A. Zaid, S. Merdas, Muntaha Y. Hayal","doi":"10.21608/jbaar.2021.178505","DOIUrl":"https://doi.org/10.21608/jbaar.2021.178505","url":null,"abstract":"Corrosion is the most important and costly cause of severe operational problems encountered in oil and gas production systems. It may occur anywhere in the production system, from the oil/gas well bottom to the final transfer of produced gas or oil to the refinery. Internal corrosion of carbon steel pipelines is a common and serious problem in oil and gas production, which is designed for long-term operation. It involves an interaction between the metal wall and the flowing fluids. This problem has caused the consideration of many corrosion control programs and research in various oilfields around the world. There is global use of carbon steels as materials of construction for pipes in the oil and gas industries. Compounds triazoles and their derivatives have attracted huge attention due to their simple preparation and superior corrosion inhibition activity towards mild steel in acidic environments. Therefore, the present review aims to highlight the carried out using Compounds triazoles and their derivatives for steel protection in different corrosive environments.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82990524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-20DOI: 10.21608/JBAAR.2020.135612
S. Youssef, E. Abbas, Yasser Hamada Ahmed Aly, S. Seif
Objectives: To detect the correlation between the single nucleotide polymorphisms (SNPs) in MBOAT7 and PNPLA3 genes and hepatic fibrosis in hepatitis C virus (HCV) Egyptian patients, and to highlight the additive effect, if any, of MBOAT7 on the correlation of PNPLA3 polymorphism with liver fibrosis in HCV patients from Egypt. Methods: In this cross-sectional study, we recruited treatment-naive patients with chronic HCV. The rs738409 (PNPLA3) and rs641738 (MBOAT7) polymorphisms were assessed by Real-Time PCR. We utilized the METAVIR‐Score to classify the degree of hepatic fibrosis and necroinflammatory activity. Results: A total of 93 patients (mean age 42.72 ± 10.46; males = 49.5%) were included. Our analysis showed that 10.8% of the patients had GG genotype of the PNPLA3 gene and 46.2% had TT genotype of the MBOAT7 gene. Compared to combined CC and GC genotypes, carriers of GG genotype in PNPLA3 gene were more likely to be males (p =0.041), have higher fibrosis grade (p =0.043), have higher serum creatinine (p =0.036), higher TSH (p =0.017) and higher viral load (p =0.045). Notably, we found a significant association between TT genotype in MBOAT7 and advanced fibrosis only (but not with necroinflammation (p >0.05). Our multivariate analysis showed that the GG genotype in the PNPLA3 gene and TT genotype in the MBOAT7 gene were independent predictors of advanced fibrosis. Conclusion: PNPLA3 GG genotype and MBOAT7 TT genotype are independent predictors for hepatic fibrosis, and thus might be linked to faster disease progression.
{"title":"The Correlation between Single Nucleotide Polymorphism of MBOAT7 and PNPLA3 Genes to The Degree of Hepatic Fibrosis in HCV Patients: An Experience from Egypt","authors":"S. Youssef, E. Abbas, Yasser Hamada Ahmed Aly, S. Seif","doi":"10.21608/JBAAR.2020.135612","DOIUrl":"https://doi.org/10.21608/JBAAR.2020.135612","url":null,"abstract":"Objectives: To detect the correlation between the single nucleotide polymorphisms (SNPs) in MBOAT7 and PNPLA3 genes and hepatic fibrosis in hepatitis C virus (HCV) Egyptian patients, and to highlight the additive effect, if any, of MBOAT7 on the correlation of PNPLA3 polymorphism with liver fibrosis in HCV patients from Egypt. Methods: In this cross-sectional study, we recruited treatment-naive patients with chronic HCV. The rs738409 (PNPLA3) and rs641738 (MBOAT7) polymorphisms were assessed by Real-Time PCR. We utilized the METAVIR‐Score to classify the degree of hepatic fibrosis and necroinflammatory activity. Results: A total of 93 patients (mean age 42.72 ± 10.46; males = 49.5%) were included. Our analysis showed that 10.8% of the patients had GG genotype of the PNPLA3 gene and 46.2% had TT genotype of the MBOAT7 gene. Compared to combined CC and GC genotypes, carriers of GG genotype in PNPLA3 gene were more likely to be males (p =0.041), have higher fibrosis grade (p =0.043), have higher serum creatinine (p =0.036), higher TSH (p =0.017) and higher viral load (p =0.045). Notably, we found a significant association between TT genotype in MBOAT7 and advanced fibrosis only (but not with necroinflammation (p >0.05). Our multivariate analysis showed that the GG genotype in the PNPLA3 gene and TT genotype in the MBOAT7 gene were independent predictors of advanced fibrosis. Conclusion: PNPLA3 GG genotype and MBOAT7 TT genotype are independent predictors for hepatic fibrosis, and thus might be linked to faster disease progression.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88353993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/jbaar.2020.116134
S. El-Masry, S. Elsherbini, A. Abdelmoneam, A. Radwan
1-Molecular Biology department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Egypt, 32897 2Haemato-Oncology department, Faculty of Medicine, Banha University, Egypt, 13511 Corresponding author: Ahmed Gouda Ibrahim Radwan, Molecular Biology department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Egypt, 32897. dr.ahmed_goda88@yahoo.com Received: May 16, 2020. Accepted: June 9, 2020. Published: June 14, 2020 DOI: 10.21608/jbaar.2020.116134
通讯作者:Ahmed Gouda Ibrahim Radwan，埃及萨达特城市大学遗传工程与生物技术研究所分子生物系，埃及，32897dr.ahmed_goda88@yahoo.com收稿日期:2020年5月16日。录用日期:2020年6月9日。发布日期:2020年6月14日DOI: 10.21608/jbaar.2020.116134
{"title":"Role of Human Pituitary Tumor-Transforming Gene1 (HPTTG1) as a prognostic biomarker for metastasis in Egyptian patients breast, colon and Liver cancer","authors":"S. El-Masry, S. Elsherbini, A. Abdelmoneam, A. Radwan","doi":"10.21608/jbaar.2020.116134","DOIUrl":"https://doi.org/10.21608/jbaar.2020.116134","url":null,"abstract":"1-Molecular Biology department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Egypt, 32897 2Haemato-Oncology department, Faculty of Medicine, Banha University, Egypt, 13511 Corresponding author: Ahmed Gouda Ibrahim Radwan, Molecular Biology department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Egypt, 32897. dr.ahmed_goda88@yahoo.com Received: May 16, 2020. Accepted: June 9, 2020. Published: June 14, 2020 DOI: 10.21608/jbaar.2020.116134","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83013860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/jbaar.2020.116129
Y. N'guessan, W. Tiekpa, Mandé Adélaïde Doue, Cassime Tiémoko, Chérif Moustapha Karamoko, A. Bidie, D. Kone
Blighia sapida is a plant with many therapeutic virtues. In Ivory Coast, this plant is widespread in several regions with different climatic conditions. The aim of our study is to compare the effects of aqueous extracts of Blighia sapida harvested in three Ivorian regions on the biochemical parameters of rats made hypertensive by a fructose diet. Rats (20) were fed a high fructose diet (70% fructose, 10% fat, 20% protein) for 30 days. Then blood pressure was measured from an armband to the tail of the rats. Finally, biochemical and lipid parameters were measured before and after the fructose-enriched diet. All rats fed the high-fructose diet had similar rates of hypertension (SBP = 160.0 ± 0.07 mmHg; DBP = 141.5 ± 1.21 mmHg; HR = 325.7 ± 1.52 beats/min) and a change in dosing parameters. Aqueous extracts of Blighia sapida from Adzopé (EAA) and Korhogo (EAK) administered to hypertensive rats normalized the previously increased cardiovascular, biochemical, and lipid parameters compared to control rats (healthy and untreated diseased rats). However, the aqueous extract of Blighia sapida of Adzopé (EAA) showed the best effects on hypertension compared to the aqueous extract of Blighia sapida of Korhogo (EAK). Indeed, the more humid the region and the climate, the more effective the extract is. The regulating effect of aqueous extracts of Blighia sapida on blood pressure, therefore, differs from one region to another with different climatic conditions. This would explain its use in traditional medicine in certain climatic zones compared to others where its therapeutic effects would be unknown because less. Adzopé would, therefore, be the best region where Blighia sapida should be harvested for the optimal treatment of high blood pressure.
{"title":"Comparative effect of extracts of Blighia sapida (Sapindaceae) from three regions on the biochemical parameters of hypertensive rats","authors":"Y. N'guessan, W. Tiekpa, Mandé Adélaïde Doue, Cassime Tiémoko, Chérif Moustapha Karamoko, A. Bidie, D. Kone","doi":"10.21608/jbaar.2020.116129","DOIUrl":"https://doi.org/10.21608/jbaar.2020.116129","url":null,"abstract":"Blighia sapida is a plant with many therapeutic virtues. In Ivory Coast, this plant is widespread in several regions with different climatic conditions. The aim of our study is to compare the effects of aqueous extracts of Blighia sapida harvested in three Ivorian regions on the biochemical parameters of rats made hypertensive by a fructose diet. Rats (20) were fed a high fructose diet (70% fructose, 10% fat, 20% protein) for 30 days. Then blood pressure was measured from an armband to the tail of the rats. Finally, biochemical and lipid parameters were measured before and after the fructose-enriched diet. All rats fed the high-fructose diet had similar rates of hypertension (SBP = 160.0 ± 0.07 mmHg; DBP = 141.5 ± 1.21 mmHg; HR = 325.7 ± 1.52 beats/min) and a change in dosing parameters. Aqueous extracts of Blighia sapida from Adzopé (EAA) and Korhogo (EAK) administered to hypertensive rats normalized the previously increased cardiovascular, biochemical, and lipid parameters compared to control rats (healthy and untreated diseased rats). However, the aqueous extract of Blighia sapida of Adzopé (EAA) showed the best effects on hypertension compared to the aqueous extract of Blighia sapida of Korhogo (EAK). Indeed, the more humid the region and the climate, the more effective the extract is. The regulating effect of aqueous extracts of Blighia sapida on blood pressure, therefore, differs from one region to another with different climatic conditions. This would explain its use in traditional medicine in certain climatic zones compared to others where its therapeutic effects would be unknown because less. Adzopé would, therefore, be the best region where Blighia sapida should be harvested for the optimal treatment of high blood pressure.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88226755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-28DOI: 10.21608/jbaar.2019.248623
A. E. El-Beltagy, Bahgat Abdelaziz, Karoline K Abdelaziz, Mamdouh Elsawy, Reham Ghanem
Background: Cadmium (Cd) is an industrial and environmental pollutant that exerts adverse effects on different organs in humans and animals. Endocrine organs, such as the thyroid, kidneys, testis, and placenta, are sensitive to the toxic effects of Cd. Ziziphus spina-christi fruit has been claimed to exhibit potent anti-oxidative and free radical scavenging abilities. Aim : This work is mainly designed to evaluate the potential modulatory role of Z. spina-christi fruit extract against thyroid, kidney, and testicular impairments associated with cadmium chloride (Cd Cl2) exposure in rats. Material & Methods : Twenty-four male rats were divided into four groups (n = 6): group I served as control, group II was supplemented daily with Ziziphus fruit extract (200 mg/kg b.wt), group III was treated with CdCl2 (5 mg/kg b.wt) each other day, and group IV was administered with CdCl2 simultaneously with Ziziphus fruit extract. The experiment was conducted for 45 days. At the end of the experiment, the rats of all groups were weighed and sacrificed, and the thyroid, kidneys, and testes were immediately excised, and processed for histological and biochemical evaluation of antioxidants and apoptosis. Also, blood samples were collected and analyzed to estimate the appropriate biochemical parameters for each organ. Results: In CdCl2 treated rats, the levels of serum hormones (TSH, T3, T4, FSH, LH, and testosterone) were significantly decreased if compared with control. Additionally, the levels of calcium, phosphorus ions, and albumin were also decreased while the level of total cholesterol was significantly elevated compared with the control. Moreover, the levels SOD and GSH were significantly lowered while the level of MDA was elevated in the tissues of all target organs (thyroid, kidney, and testes). Also, the sections from the thyroid, kidney, and testes displayed pronounced deleterious histological changes. Furthermore, the mean % values of positively expressed cells for caspase-3 and annexin-v markers were significantly elevated in the thyroid and testicular tissues of CdCl2 received rats compared to control. Co-supplementation of Z. spina-christi fruit extract to CdCl2 exposed rats successfully restored the altered biochemical and histological and apoptotic changes induced by cadmium . Conclusion: It is concluded that Z. spina-christi fruit extract has a powerful protective role against Cd-induced deleterious biochemical and histological changes in the thyroid, kidneys, and testes of male rats.
{"title":"Adverse effects of cadmium on the thyroid, kidneys, and testes in Wistar albino rats and the possible modulatory role of Zizyphus spina-christi (Sidr) fruit extract (Histological and biochemical studies)","authors":"A. E. El-Beltagy, Bahgat Abdelaziz, Karoline K Abdelaziz, Mamdouh Elsawy, Reham Ghanem","doi":"10.21608/jbaar.2019.248623","DOIUrl":"https://doi.org/10.21608/jbaar.2019.248623","url":null,"abstract":"Background: Cadmium (Cd) is an industrial and environmental pollutant that exerts adverse effects on different organs in humans and animals. Endocrine organs, such as the thyroid, kidneys, testis, and placenta, are sensitive to the toxic effects of Cd. Ziziphus spina-christi fruit has been claimed to exhibit potent anti-oxidative and free radical scavenging abilities. Aim : This work is mainly designed to evaluate the potential modulatory role of Z. spina-christi fruit extract against thyroid, kidney, and testicular impairments associated with cadmium chloride (Cd Cl2) exposure in rats. Material & Methods : Twenty-four male rats were divided into four groups (n = 6): group I served as control, group II was supplemented daily with Ziziphus fruit extract (200 mg/kg b.wt), group III was treated with CdCl2 (5 mg/kg b.wt) each other day, and group IV was administered with CdCl2 simultaneously with Ziziphus fruit extract. The experiment was conducted for 45 days. At the end of the experiment, the rats of all groups were weighed and sacrificed, and the thyroid, kidneys, and testes were immediately excised, and processed for histological and biochemical evaluation of antioxidants and apoptosis. Also, blood samples were collected and analyzed to estimate the appropriate biochemical parameters for each organ. Results: In CdCl2 treated rats, the levels of serum hormones (TSH, T3, T4, FSH, LH, and testosterone) were significantly decreased if compared with control. Additionally, the levels of calcium, phosphorus ions, and albumin were also decreased while the level of total cholesterol was significantly elevated compared with the control. Moreover, the levels SOD and GSH were significantly lowered while the level of MDA was elevated in the tissues of all target organs (thyroid, kidney, and testes). Also, the sections from the thyroid, kidney, and testes displayed pronounced deleterious histological changes. Furthermore, the mean % values of positively expressed cells for caspase-3 and annexin-v markers were significantly elevated in the thyroid and testicular tissues of CdCl2 received rats compared to control. Co-supplementation of Z. spina-christi fruit extract to CdCl2 exposed rats successfully restored the altered biochemical and histological and apoptotic changes induced by cadmium . Conclusion: It is concluded that Z. spina-christi fruit extract has a powerful protective role against Cd-induced deleterious biochemical and histological changes in the thyroid, kidneys, and testes of male rats.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77282186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-11DOI: 10.21608/jbaar.2019.114536
M. Nanekely, F. Al-Faraj, M. Scholz
{"title":"Estimating Groundwater Balance in the Presence of Climate Change Impact: A Case Study of Semi-Arid Area","authors":"M. Nanekely, F. Al-Faraj, M. Scholz","doi":"10.21608/jbaar.2019.114536","DOIUrl":"https://doi.org/10.21608/jbaar.2019.114536","url":null,"abstract":"","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77130708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.21608/jbaar.2019.115570
M. Nasr, E. Hassan, GhadaS. Hassan, A. Ibrahim, Salwa E Mohamed, Amal M. Abdel-Aziz
Background: Globally Helicobacter Pylori (H. Pylori) infection and liver diseases are renowned with their high incidence rate. Recent studies have observed the relation between H. Pylori infection and liver disease progression to cirrhosis. This study aimed to determine the prevalence of H. pylori in liver cirrhotic patients. Patient and Methods: This study was conducted on 100 liver cirrhotic patients to detect H. Pylori infection based on UreA gene detection in ascites using nested polymerase chain reaction (nPCR). Biochemical analysis was performed on ascitic fluid, serum, complete blood count (CBC), and polymorph nuclear leukocytes count (PMN). Result: H. Pylori infection was detected in 75% of patients with liver cirrhosis. In ascitic fluid, H. pylori infection showed a highly significant association with albumin and PMN (P=0.0001) and also with other biochemical analyses including serum ascites albumin gradient (SAAG), specific gravity, and glucose (P=0.005, 0.0031, and 0.02, respectively). In serum only, the infection showed significant association with alkaline phosphatase, albumin, and hemoglobin with P values of 0.008, 0.014, and 0.008, respectively. Conclusion: The current study results shed light on the significant role of H. Pylori infection in ascites of patients with decompensated end-stage liver cirrhosis suggesting a prospective relation between H. pylori infection and liver diseases progression due to hepatic infections especially HCV.
{"title":"Molecular screening of Helicobacter pylori among Egyptian patients with decompensated liver cirrhosis","authors":"M. Nasr, E. Hassan, GhadaS. Hassan, A. Ibrahim, Salwa E Mohamed, Amal M. Abdel-Aziz","doi":"10.21608/jbaar.2019.115570","DOIUrl":"https://doi.org/10.21608/jbaar.2019.115570","url":null,"abstract":"Background: Globally Helicobacter Pylori (H. Pylori) infection and liver diseases are renowned with their high incidence rate. Recent studies have observed the relation between H. Pylori infection and liver disease progression to cirrhosis. This study aimed to determine the prevalence of H. pylori in liver cirrhotic patients. Patient and Methods: This study was conducted on 100 liver cirrhotic patients to detect H. Pylori infection based on UreA gene detection in ascites using nested polymerase chain reaction (nPCR). Biochemical analysis was performed on ascitic fluid, serum, complete blood count (CBC), and polymorph nuclear leukocytes count (PMN). Result: H. Pylori infection was detected in 75% of patients with liver cirrhosis. In ascitic fluid, H. pylori infection showed a highly significant association with albumin and PMN (P=0.0001) and also with other biochemical analyses including serum ascites albumin gradient (SAAG), specific gravity, and glucose (P=0.005, 0.0031, and 0.02, respectively). In serum only, the infection showed significant association with alkaline phosphatase, albumin, and hemoglobin with P values of 0.008, 0.014, and 0.008, respectively. Conclusion: The current study results shed light on the significant role of H. Pylori infection in ascites of patients with decompensated end-stage liver cirrhosis suggesting a prospective relation between H. pylori infection and liver diseases progression due to hepatic infections especially HCV.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75391388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.21608/jbaar.2019.115571
Johnson Olaleye Oladele, O. Oyewole, M. O. Oyeleke, O. Adewale, Oluwamuyiwa Emmanuel Adeloju
Cadmium is a known industrial toxicant that accumulates in the kidney and causes oxidative damage. The present study was carried out to evaluate the attenuating effects of Annona muricata seed extracts against cadmium mediated renal toxicity in Wistar rats. Twenty-eight (28) Wistar albino rats sorted into four groups of seven rats per group were used in this study. Group A serves as the control and received distilled water orally. Group B, C, and D were administered 5 mg/kg body weight cadmium chloride with Group C and D were treated daily with 200 and 500 mg/kg body weight of ethanolic seed extract of Annona muricata (ESEAM)respectively by oral administration while group B was left untreated for 14 days. Results obtained showed that cadmium significantly (P<0.05) increased the serum level of urea and creatinine. Oxidative stress was induced by cadmium with a significant increase in MDA level and decrease concentration in ascorbic acid, GSH, GPx, SOD, and CAT in the kidney. Efficacy of Annona muricata seed extracts against renal toxicity was evaluated in terms of biochemical estimation of enzymatic and non-enzymatic antioxidant activities, histopathological changes, and rate of lipid peroxidation. Treatment with ESEAM prevented deteriorative effects induced by Cd through a protective mechanism that involved the reduction of increased oxidative stress.
{"title":"Annona muricata Attenuates Cadmium-Induced Oxidative Stress and Renal Toxicity in Wistar Rats","authors":"Johnson Olaleye Oladele, O. Oyewole, M. O. Oyeleke, O. Adewale, Oluwamuyiwa Emmanuel Adeloju","doi":"10.21608/jbaar.2019.115571","DOIUrl":"https://doi.org/10.21608/jbaar.2019.115571","url":null,"abstract":"Cadmium is a known industrial toxicant that accumulates in the kidney and causes oxidative damage. The present study was carried out to evaluate the attenuating effects of Annona muricata seed extracts against cadmium mediated renal toxicity in Wistar rats. Twenty-eight (28) Wistar albino rats sorted into four groups of seven rats per group were used in this study. Group A serves as the control and received distilled water orally. Group B, C, and D were administered 5 mg/kg body weight cadmium chloride with Group C and D were treated daily with 200 and 500 mg/kg body weight of ethanolic seed extract of Annona muricata (ESEAM)respectively by oral administration while group B was left untreated for 14 days. Results obtained showed that cadmium significantly (P<0.05) increased the serum level of urea and creatinine. Oxidative stress was induced by cadmium with a significant increase in MDA level and decrease concentration in ascorbic acid, GSH, GPx, SOD, and CAT in the kidney. Efficacy of Annona muricata seed extracts against renal toxicity was evaluated in terms of biochemical estimation of enzymatic and non-enzymatic antioxidant activities, histopathological changes, and rate of lipid peroxidation. Treatment with ESEAM prevented deteriorative effects induced by Cd through a protective mechanism that involved the reduction of increased oxidative stress.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77963527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.21608/jbaar.2019.115437
Y. Adjovi, Prudence Agnandji, Lucie Ayi-fanou, A. Sanni
Legumes such as cowpea (Vigna ungiculata) and Bambara groundnut (Vigna subterranean) are the most sought in Benin and in the West Africa sub-region. These legumes are an important protein source because of their high content of essentials amino acids. But, like many dried agricultural and food products, they are subject to contamination by molds and mycotoxins during storage. In order to assess the contamination of Bambara groundnuts, by the most dangerous and widespread mycotoxins in Africa (aflatoxins), especially in Benin, this study was conducted in Cotonou (Benin) main markets. Thirty-five (35) samples of Bambara groundnuts were collected from six (6) different markets in Cotonou. The fungi are isolated and identified morphologically on different media after direct culture. The species of Aspergillus section Flavi aflatoxigenic potential are evaluated and the contamination of samples by aflatoxins was determined by Thin Layer Chromatography (TLC). The results of this study showed that the average water content of Bambara groundnuts samples ranged from 4.85 to 9.35%. The main species of mold isolated are Mucor spp., Rhizopus spp., Alternaria sp., and Aspergillus spp. Forty-six (46) strains of Aspergillus section Flavi were isolated on Potatoes Dextrose Agar (PDA). All of them have been identified as aflatoxigenic strains and were divided into different groups according to their morphological appearance and secondary metabolites. Twentytwo samples (62.85%) of Bambara groundnuts were contaminated by aflatoxins. This study showed that Bambara groundnuts which sold in the main markets of Cotonou constitute a health risk for populations.
{"title":"Isolation of Aspergillus section Flavi and determination of aflatoxins in Bambara groundnut sold in Cotonou main markets (Benin)","authors":"Y. Adjovi, Prudence Agnandji, Lucie Ayi-fanou, A. Sanni","doi":"10.21608/jbaar.2019.115437","DOIUrl":"https://doi.org/10.21608/jbaar.2019.115437","url":null,"abstract":"Legumes such as cowpea (Vigna ungiculata) and Bambara groundnut (Vigna subterranean) are the most sought in Benin and in the West Africa sub-region. These legumes are an important protein source because of their high content of essentials amino acids. But, like many dried agricultural and food products, they are subject to contamination by molds and mycotoxins during storage. In order to assess the contamination of Bambara groundnuts, by the most dangerous and widespread mycotoxins in Africa (aflatoxins), especially in Benin, this study was conducted in Cotonou (Benin) main markets. Thirty-five (35) samples of Bambara groundnuts were collected from six (6) different markets in Cotonou. The fungi are isolated and identified morphologically on different media after direct culture. The species of Aspergillus section Flavi aflatoxigenic potential are evaluated and the contamination of samples by aflatoxins was determined by Thin Layer Chromatography (TLC). The results of this study showed that the average water content of Bambara groundnuts samples ranged from 4.85 to 9.35%. The main species of mold isolated are Mucor spp., Rhizopus spp., Alternaria sp., and Aspergillus spp. Forty-six (46) strains of Aspergillus section Flavi were isolated on Potatoes Dextrose Agar (PDA). All of them have been identified as aflatoxigenic strains and were divided into different groups according to their morphological appearance and secondary metabolites. Twentytwo samples (62.85%) of Bambara groundnuts were contaminated by aflatoxins. This study showed that Bambara groundnuts which sold in the main markets of Cotonou constitute a health risk for populations.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72867856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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