Pub Date : 2021-12-12DOI: 10.21608/jbaar.2021.209005
Asmaa A Abdeltawab, Hassan I H Elsyyad, Karoline K Abdelaziz, A. E. El-Beltagy
Aim: To evaluate the potential role of quercetin against N-methyl nitrosourea (MNU)-induced hepatocellular carcinoma (HCC) in pregnant rats and their offspring. Material & Methods: Twenty-four female rats were used in this study. Six rats were preserved without treatment and the other eighteen female rats were induced by a single dose of MNU (50 mg /kg B wt). After confirmation of the positive tumor marker test, female rats were placed with the males for mating. The pregnant rats were divided into four groups (n=6). Group1: control rats, group2: Quercetin supplemented rats (20 mg/kg B.Wt, group3: MNU-induced rats, and group4: MNUtreated rats followed by supplementation with quercetin. At the end of the weaning period, the mothers and their offspring (at 21 days old postnatal) of all groups were sacrificed, the liver was removed immediately for histological and immunohistochemical investigations. Also, blood samples were collected, centrifuged, and processed for the estimation of antioxidants. Results: In the control and quercetin groups, the histological investigation of the liver of mother rats and their offspring appeared with normal architecture. In Group3 (MNU-induced group) the liver sections of mother's rats revealed degenerated hepatic lobules with pronounced cellular hyperplasia (HCC) especially around the central vein and portal area as well as numerous Kupffer cells and fat droplets. However, the liver sections of offspring displayed little cellular hyperplasia but the central and portal veins appeared damaged and congested with blood. Immunohistochemically, the liver sections of MNU-induced mother's rats and their offspring appeared strongly stained with α-FP antibody and negatively stained with caspase-3 antibody. Furthermore, the levels of serum antioxidants; SOD, CAT&GSH were significantly decreased however the levels of MDA and NO were significantly increased in MNU-induced mother rats and their offspring if compared with control. In group 4, quercetin was able to restore the histological and immunohistochemical changes in the liver induced by MNU. Also, the levels of antioxidants, as well as MDA and NO, were markedly restored near to the normal level as in control. Conclusion: Quercetin has a powerful therapeutic role against MNU-induced HCC in pregnant rats and their offspring.
目的:探讨槲皮素对n -甲基亚硝基脲(MNU)诱导的妊娠大鼠及其子代肝癌的潜在作用。材料与方法:选用24只雌性大鼠。保留6只大鼠，不处理，其余18只雌性大鼠用单剂量MNU (50 mg /kg B wt)诱导。肿瘤标志物检测确认阳性后，将雌性大鼠与雄性大鼠配对。将妊娠大鼠分为4组(n=6)。组1:对照组大鼠，组2:槲皮素添加大鼠(20 mg/kg B.Wt)，组3:mnu诱导大鼠，组4:mnu处理后补充槲皮素。断奶结束后，处死各组母鼠及其后代(出生后21日龄)，立即取肝进行组织学和免疫组织化学检查。同时，采集血样，离心，并处理抗氧化剂的估计。结果:对照组和槲皮素组大鼠及其子代肝脏组织学检查显示肝脏结构正常。第3组(mnu诱导组)母鼠肝脏切片显示肝小叶变性，肝细胞增生明显，特别是在中央静脉和门静脉区周围，并可见大量库普弗细胞和脂肪滴。然而，子代肝脏切片显示少量细胞增生，但中央静脉和门静脉出现损伤和充血。免疫组化结果显示，mnu诱导的母鼠及其子鼠肝脏组织出现α-FP抗体强染色，caspase-3抗体阴性染色。此外，血清抗氧化剂水平;与对照组相比，mnu诱导的母鼠及其子鼠的SOD、cat和gsh水平显著降低，而MDA和NO水平显著升高。在第4组，槲皮素能够恢复MNU诱导的肝脏组织学和免疫组化变化。此外，抗氧化剂水平，以及MDA和NO，明显恢复到接近正常水平的控制。结论:槲皮素对mnu诱导的妊娠大鼠及其子代肝癌有较强的治疗作用。
{"title":"Therapeutic Role of Quercetin against Experimentally Induced Hepatocellular Carcinoma in Female Albino Rats and their offspring","authors":"Asmaa A Abdeltawab, Hassan I H Elsyyad, Karoline K Abdelaziz, A. E. El-Beltagy","doi":"10.21608/jbaar.2021.209005","DOIUrl":"https://doi.org/10.21608/jbaar.2021.209005","url":null,"abstract":"Aim: To evaluate the potential role of quercetin against N-methyl nitrosourea (MNU)-induced hepatocellular carcinoma (HCC) in pregnant rats and their offspring. Material & Methods: Twenty-four female rats were used in this study. Six rats were preserved without treatment and the other eighteen female rats were induced by a single dose of MNU (50 mg /kg B wt). After confirmation of the positive tumor marker test, female rats were placed with the males for mating. The pregnant rats were divided into four groups (n=6). Group1: control rats, group2: Quercetin supplemented rats (20 mg/kg B.Wt, group3: MNU-induced rats, and group4: MNUtreated rats followed by supplementation with quercetin. At the end of the weaning period, the mothers and their offspring (at 21 days old postnatal) of all groups were sacrificed, the liver was removed immediately for histological and immunohistochemical investigations. Also, blood samples were collected, centrifuged, and processed for the estimation of antioxidants. Results: In the control and quercetin groups, the histological investigation of the liver of mother rats and their offspring appeared with normal architecture. In Group3 (MNU-induced group) the liver sections of mother's rats revealed degenerated hepatic lobules with pronounced cellular hyperplasia (HCC) especially around the central vein and portal area as well as numerous Kupffer cells and fat droplets. However, the liver sections of offspring displayed little cellular hyperplasia but the central and portal veins appeared damaged and congested with blood. Immunohistochemically, the liver sections of MNU-induced mother's rats and their offspring appeared strongly stained with α-FP antibody and negatively stained with caspase-3 antibody. Furthermore, the levels of serum antioxidants; SOD, CAT&GSH were significantly decreased however the levels of MDA and NO were significantly increased in MNU-induced mother rats and their offspring if compared with control. In group 4, quercetin was able to restore the histological and immunohistochemical changes in the liver induced by MNU. Also, the levels of antioxidants, as well as MDA and NO, were markedly restored near to the normal level as in control. Conclusion: Quercetin has a powerful therapeutic role against MNU-induced HCC in pregnant rats and their offspring.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74855806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-11DOI: 10.21608/jbaar.2021.208849
A. Guirgis, D. Habib, H. Hanna, R. Mahmoud
Molecular Biology Department, Genetic Engineering &Biotechnology Research Institute University of Sadat City, Egypt 2 Clinical Biochemistry Department, National Research Centre (NRC), Egypt 3 Chemical Pathology Department, Faculty of Physical Therapy, Pharos University in Alexandria, Egypt DOI: 10.21608/jbaar.2021.208849 Abstract Background: The number of individuals with diabetes mellitus (DM) worldwide has more than doubled over the past three decades, and it has been predicted that the number of diabetic patients would increase to 439 million by 2030, so many efforts are being made to find a new and effective treatment for diabetes mellitus. Objective: This work aims to study the biochemical and molecular effect of the Moringa oleifera MO and Ficus sycomorus FLE in streptozotocin-induced diabetic rats and compare them with the effect of metformin, by estimation of the expression of β-actin and glucose transporter GLUT2, GLUT4, and Insulin Receptors genes in studied groups, Determination of fasting blood glucose and insulin levels before and after induction of (STZ), Quantitative estimation serum cholesterol, TG levels, HDL, LDL, Some antioxidant enzyme activity Glutathione peroxidase, catalase and lipid peroxidation in plasma. Results: MO and FLE showed promising anti-diabetic potential in diabetic-bearing albino mice which can be attributed to its anti-inflammatory effect. This could serve as a stepping stone towards the discovery of newer safe and effective anti-diabetic treatment.
{"title":"Molecular and Biochemical Studies on Some Natural Compounds and Their Effect on the Streptozotocin-induced Diabetic Rats and Their Role in Treatment","authors":"A. Guirgis, D. Habib, H. Hanna, R. Mahmoud","doi":"10.21608/jbaar.2021.208849","DOIUrl":"https://doi.org/10.21608/jbaar.2021.208849","url":null,"abstract":"Molecular Biology Department, Genetic Engineering &Biotechnology Research Institute University of Sadat City, Egypt 2 Clinical Biochemistry Department, National Research Centre (NRC), Egypt 3 Chemical Pathology Department, Faculty of Physical Therapy, Pharos University in Alexandria, Egypt DOI: 10.21608/jbaar.2021.208849 Abstract Background: The number of individuals with diabetes mellitus (DM) worldwide has more than doubled over the past three decades, and it has been predicted that the number of diabetic patients would increase to 439 million by 2030, so many efforts are being made to find a new and effective treatment for diabetes mellitus. Objective: This work aims to study the biochemical and molecular effect of the Moringa oleifera MO and Ficus sycomorus FLE in streptozotocin-induced diabetic rats and compare them with the effect of metformin, by estimation of the expression of β-actin and glucose transporter GLUT2, GLUT4, and Insulin Receptors genes in studied groups, Determination of fasting blood glucose and insulin levels before and after induction of (STZ), Quantitative estimation serum cholesterol, TG levels, HDL, LDL, Some antioxidant enzyme activity Glutathione peroxidase, catalase and lipid peroxidation in plasma. Results: MO and FLE showed promising anti-diabetic potential in diabetic-bearing albino mice which can be attributed to its anti-inflammatory effect. This could serve as a stepping stone towards the discovery of newer safe and effective anti-diabetic treatment.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88695403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/jbaar.2021.209054
R. El-Leithy, A. Mohamed, Asmaa EL-refaay, Mohamed Omran
Imatinib mesylate and dipyridamole were antineoplastic targeted chemotherapeutic agents, and it is here evaluating the anticancer of imatinib mesylate and dipyridamole compounds against MDA-MB231 breast cancer cell line in-vitro and the related cell cycle and gene profile. MDA-MB231 cells showed more sensitivity to imatinib mesylate than to dipyridamole, with an IC50 value of 348 g/mL versus 494 g/mL for imatinib mesylate and dipyridamole, respectively (P-value < 0.05). The up/downregulation of Bax and Bcl2 and metastasis contributing gene (MMP-1) assured the anticancer activity. Also, the apoptotic potential of dipyridamole and imatinib mesylate was verified by arresting cells in the G2/M phase and increasing the percentage of the apoptotic cells in the pre-G1 phase. The antioxidant levels were drug dependent, as they were significantly higher(P<0.05) in cells treated with imatinib than in cells treated with Dipyridamole. Conclusion: Imatinib mesylate growth-inhibitory impact on breast cell lines, either alone or in combination with dipyridamole, may be mediated through up/downregulation of the Bax, Bcl-2, and MMP-1 genes. Imatinib is a promising treatment for breast cancer patients who require targeted therapy.
{"title":"Evaluation of the anticancer activity of dipyridamole and Imatinib mesylate compounds against breast cancer cell line and related biochemical and genetic changes","authors":"R. El-Leithy, A. Mohamed, Asmaa EL-refaay, Mohamed Omran","doi":"10.21608/jbaar.2021.209054","DOIUrl":"https://doi.org/10.21608/jbaar.2021.209054","url":null,"abstract":"Imatinib mesylate and dipyridamole were antineoplastic targeted chemotherapeutic agents, and it is here evaluating the anticancer of imatinib mesylate and dipyridamole compounds against MDA-MB231 breast cancer cell line in-vitro and the related cell cycle and gene profile. MDA-MB231 cells showed more sensitivity to imatinib mesylate than to dipyridamole, with an IC50 value of 348 g/mL versus 494 g/mL for imatinib mesylate and dipyridamole, respectively (P-value < 0.05). The up/downregulation of Bax and Bcl2 and metastasis contributing gene (MMP-1) assured the anticancer activity. Also, the apoptotic potential of dipyridamole and imatinib mesylate was verified by arresting cells in the G2/M phase and increasing the percentage of the apoptotic cells in the pre-G1 phase. The antioxidant levels were drug dependent, as they were significantly higher(P<0.05) in cells treated with imatinib than in cells treated with Dipyridamole. Conclusion: Imatinib mesylate growth-inhibitory impact on breast cell lines, either alone or in combination with dipyridamole, may be mediated through up/downregulation of the Bax, Bcl-2, and MMP-1 genes. Imatinib is a promising treatment for breast cancer patients who require targeted therapy.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88120864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/jbaar.2021.251237
A. Yameny
: Background: Blood leukocytes are an important part of the body’s defense system, and infection status can be predicted by measuring WBC levels . COVID-19 may involve many organ systems in its host. Studies suggest that hematological profiles change during SARS-CoV-2 illness. Patients and methods: This study included 504 mild infected patients with confirmed COVID-19 infection, these study subjects were randomly selected irrespective of the age group and both genders were included, EDTA blood sample was collected for performing total and differential white blood cells (Diagon D-cell 60 hematology analyzer Europe-Diagon Ltd. Hungary). Results: The present study included patients aged from14 years to 75 years mean age was 44.5 ±30.5 who were confirmed to have Covid-19 based on real-time reverse transcription-polymerase chain reaction, female gender was more frequent (n=280, 55.6%) than Male gender (n=224, 44.4%). This study reveals normal total WBCs count in 320 patients (63.5%), neutrophilia with a sensitivity of 77.8%, and lymphopenia with a sensitivity of 73%. Conclusion: Neutrophilia has a sensitivity of 77.8% and lymphopenia has a sensitivity of 73% for diagnosis or prognosis of mild infection of COVID-19 patients (Outpatients and patients under home observation). lymphopenia, biomarker.
{"title":"Characteristics of peripheral Leukocyte in moderate infection of COVID-19","authors":"A. Yameny","doi":"10.21608/jbaar.2021.251237","DOIUrl":"https://doi.org/10.21608/jbaar.2021.251237","url":null,"abstract":": Background: Blood leukocytes are an important part of the body’s defense system, and infection status can be predicted by measuring WBC levels . COVID-19 may involve many organ systems in its host. Studies suggest that hematological profiles change during SARS-CoV-2 illness. Patients and methods: This study included 504 mild infected patients with confirmed COVID-19 infection, these study subjects were randomly selected irrespective of the age group and both genders were included, EDTA blood sample was collected for performing total and differential white blood cells (Diagon D-cell 60 hematology analyzer Europe-Diagon Ltd. Hungary). Results: The present study included patients aged from14 years to 75 years mean age was 44.5 ±30.5 who were confirmed to have Covid-19 based on real-time reverse transcription-polymerase chain reaction, female gender was more frequent (n=280, 55.6%) than Male gender (n=224, 44.4%). This study reveals normal total WBCs count in 320 patients (63.5%), neutrophilia with a sensitivity of 77.8%, and lymphopenia with a sensitivity of 73%. Conclusion: Neutrophilia has a sensitivity of 77.8% and lymphopenia has a sensitivity of 73% for diagnosis or prognosis of mild infection of COVID-19 patients (Outpatients and patients under home observation). lymphopenia, biomarker.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80243011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-01DOI: 10.21608/jbaar.2021.251241
S. Alabd, Ahmed Mahmoud
: Background: Infection with viral agents causes upregulation of cytokines such as Tumor Necrosis Factor-alpha (TNF-α), which is considered an important mediator of inflammation. TNF-α has been associated with a poor prognosis in patients with the severe acute respiratory syndrome (SARS). Patients and methods: This study included 66 mild COVID-19 patients with confirmed COVID-19 infection and 22 healthy people as a control group, these study subjects were randomly selected irrespective of the age group and both genders were included, 1 ml blood sample was collected for performing serum TNF-α levels test, Reagents of EIAab is located at East Lake Hi-Tech Development Zone, Wuhan China. Human tumor necrosis factor ELISA kit TNF-α serum levels immunoassay test catalog number E0133h. Results: This study reveals that serum TNF-α levels for mild COVID-19 patients and healthy control people were non-significant with a p-value of 0.1191 between the two groups. Conclusion: the serum TNF-α level is not a significant biomarker for diagnosis or prognosis of mild COVID-19 patients (Outpatients and patients under home observation), while other studies reported patients with COVID-19 demonstrated significantly elevated levels of TNF- 𝛼 upon admission to hospitals.
{"title":"The association between Tumor Necrosis Factor-alpha level (TNF-α) and moderate COVID-19 patients in Egypt","authors":"S. Alabd, Ahmed Mahmoud","doi":"10.21608/jbaar.2021.251241","DOIUrl":"https://doi.org/10.21608/jbaar.2021.251241","url":null,"abstract":": Background: Infection with viral agents causes upregulation of cytokines such as Tumor Necrosis Factor-alpha (TNF-α), which is considered an important mediator of inflammation. TNF-α has been associated with a poor prognosis in patients with the severe acute respiratory syndrome (SARS). Patients and methods: This study included 66 mild COVID-19 patients with confirmed COVID-19 infection and 22 healthy people as a control group, these study subjects were randomly selected irrespective of the age group and both genders were included, 1 ml blood sample was collected for performing serum TNF-α levels test, Reagents of EIAab is located at East Lake Hi-Tech Development Zone, Wuhan China. Human tumor necrosis factor ELISA kit TNF-α serum levels immunoassay test catalog number E0133h. Results: This study reveals that serum TNF-α levels for mild COVID-19 patients and healthy control people were non-significant with a p-value of 0.1191 between the two groups. Conclusion: the serum TNF-α level is not a significant biomarker for diagnosis or prognosis of mild COVID-19 patients (Outpatients and patients under home observation), while other studies reported patients with COVID-19 demonstrated significantly elevated levels of TNF- 𝛼 upon admission to hospitals.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86451320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-26DOI: 10.21608/jbaar.2021.201445
Mohamed Omran, Yassmin Taha, M. Kadry, Fathy M Eltaweel, T. Emran, A. Tabll
Diagnostic values of a model based on B-type natriuretic peptide, C reactive protein, and neutrophil-lymphocyte ratio for diagnosis of diabetic heart diseases patients Mohamed M. Omran*, Yassmin Taha, Mohamed Kadry, Fathy M. Eltaweel, Tarek M. Emran, Ashraf A. Tabll 1 Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt. 2 Chemistry Department, Faculty of Science, Damietta University, Egypt. Laboratory Department, Gamasa Central Hospital, Gamasa, Egypt. Clinical Pathology Department, Faculty of Medicine, Al-Azhar University, New Damietta; Egypt Microbial Biotechnology Department, National Research Centre, Cairo, Egypt 6 Egypt Center for Research and Regenerative Medicine (ECRRM), Cairo, Egypt
Mohamed M. Omran*， Yassmin Taha, Mohamed Kadry, Fathy M. Eltaweel, Tarek M. Emran, Ashraf a .表1埃及Helwan大学化学系，开罗2埃及Damietta大学化学系，开罗埃及加马萨中心医院检验科。爱资哈尔大学医学院临床病理学系，新达米埃塔;6埃及研究与再生医学中心(ECRRM)，埃及开罗
{"title":"Diagnostic values of a model based on B-type natriuretic peptide, C reactive protein, and neutrophil-lymphocyte ratio for diagnosis of diabetic heart diseases patients","authors":"Mohamed Omran, Yassmin Taha, M. Kadry, Fathy M Eltaweel, T. Emran, A. Tabll","doi":"10.21608/jbaar.2021.201445","DOIUrl":"https://doi.org/10.21608/jbaar.2021.201445","url":null,"abstract":"Diagnostic values of a model based on B-type natriuretic peptide, C reactive protein, and neutrophil-lymphocyte ratio for diagnosis of diabetic heart diseases patients Mohamed M. Omran*, Yassmin Taha, Mohamed Kadry, Fathy M. Eltaweel, Tarek M. Emran, Ashraf A. Tabll 1 Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt. 2 Chemistry Department, Faculty of Science, Damietta University, Egypt. Laboratory Department, Gamasa Central Hospital, Gamasa, Egypt. Clinical Pathology Department, Faculty of Medicine, Al-Azhar University, New Damietta; Egypt Microbial Biotechnology Department, National Research Centre, Cairo, Egypt 6 Egypt Center for Research and Regenerative Medicine (ECRRM), Cairo, Egypt","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86729813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-24DOI: 10.21608/jbaar.2021.201103
H. El-Borm
To date, studies on the effects of prenatal exposure to diphenylamine on developing fetuses are sparse. Therefore, further investigation is required to determine the potential prenatal hazard of this compound and to introduce possible treatment for these hazards. This study aimed to assess the biochemical, histopathological, and ultrastructural changes induced by diphenylamine in the developing liver and kidney of rat fetuses and the role of vitamin E in alleviating these changes. Fifty pregnant rats were divided equally into five groups, the group I was administrated distilled water, group II was administrated corn oil, group III was administrated 100 mg/kg/b.wt. vitamin E, group IV was administrated approximately 400 mg/kg/b.wt diphenylamine and group V was administrated diphenylamine + vitamin E at the above-mentioned doses from the 6 to 15 day of pregnancy. Diphenylamine induced undesirable histopathological and ultrastructural changes in the fetal liver and kidney. These changes were in the form of vacuolation, congestions of central veins, hemorrhage, leucocytic infiltration, degenerated cytoplasm, pyknotic nuclei, and swollen mitochondria and rER of hepatocytes. While the degenerative changes in the kidney were represented by degenerated brush border, lumen dilation, tubular hyalinization, vacuolation, degenerated nuclei, and mitochondria. Also, there was a significant decrease in the antioxidant enzymes i.e., superoxide dismutase and catalase, and a significant increase in reactive oxygen radicals and malondialdehyde. Treatment with vitamins E after diphenylamine restored all biochemical, histopathological, and ultrastructural damage cited above. In conclusion, vitamin E has antioxidant effects which could be able to antagonize diphenylamine prenatal toxicity.
{"title":"Antioxidant effect of vitamin E on diphenylamine-induced hepato-renal oxidative stress and structural changes in rat fetuses","authors":"H. El-Borm","doi":"10.21608/jbaar.2021.201103","DOIUrl":"https://doi.org/10.21608/jbaar.2021.201103","url":null,"abstract":"To date, studies on the effects of prenatal exposure to diphenylamine on developing fetuses are sparse. Therefore, further investigation is required to determine the potential prenatal hazard of this compound and to introduce possible treatment for these hazards. This study aimed to assess the biochemical, histopathological, and ultrastructural changes induced by diphenylamine in the developing liver and kidney of rat fetuses and the role of vitamin E in alleviating these changes. Fifty pregnant rats were divided equally into five groups, the group I was administrated distilled water, group II was administrated corn oil, group III was administrated 100 mg/kg/b.wt. vitamin E, group IV was administrated approximately 400 mg/kg/b.wt diphenylamine and group V was administrated diphenylamine + vitamin E at the above-mentioned doses from the 6 to 15 day of pregnancy. Diphenylamine induced undesirable histopathological and ultrastructural changes in the fetal liver and kidney. These changes were in the form of vacuolation, congestions of central veins, hemorrhage, leucocytic infiltration, degenerated cytoplasm, pyknotic nuclei, and swollen mitochondria and rER of hepatocytes. While the degenerative changes in the kidney were represented by degenerated brush border, lumen dilation, tubular hyalinization, vacuolation, degenerated nuclei, and mitochondria. Also, there was a significant decrease in the antioxidant enzymes i.e., superoxide dismutase and catalase, and a significant increase in reactive oxygen radicals and malondialdehyde. Treatment with vitamins E after diphenylamine restored all biochemical, histopathological, and ultrastructural damage cited above. In conclusion, vitamin E has antioxidant effects which could be able to antagonize diphenylamine prenatal toxicity.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82578352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.21608/jbaar.2021.200859
A. Yameny
Ahmed A. Yameny (Email: dr.ahmedyameny@yahoo.com) Tel: (002)01222708665, (002)01002112248 DOI: 10.21608/jbaar.2021.200859 Abstract: Background: Thrombocytopenia is a common manifestation and also an indicator of poor prognosis of SARS, MERS, and COVID-19 according to previous researches, Some studies have found a relationship between thrombocytopenia and the severity of the COVID-19 and related mortality. Patients and methods: This study included 504 out hospitalized patients with confirmed COVID-19 infection in Alexandria, Egypt, these study subjects were randomly selected irrespective of the age group and both genders were included, EDTA blood sample was collected for performing complete blood count and platelet count (Diagon D-cell 60 hematology analyzer Europe-Diagon Ltd. Hungary). Results: The present study included patients aged from14 years to 75 years mean age was 44.5 ±30.5 who were confirmed to have Covid-19 based on real-time reverse transcriptionpolymerase chain reaction, the female gender was more frequent (n=280, 55.6%) than Male gender (n=224, 44.4%). This study reveals a normal platelet count in 456 patients (90.5%), and a mild low platelet count of 140150× 10/L in 48 patients(9.5%), with a p-value, is 0.415 which is more than 0.05 not significant. And no patients in this studied group recorded platelet count less than 140× 10/L. Conclusion: Platelet was not a significant biomarker for COVID-19 diagnosis or prognosis in out-hospitalized patients (Outpatients and patients under home observation).
{"title":"Association between thrombocytopenia and mild infection of COVID-19 patients","authors":"A. Yameny","doi":"10.21608/jbaar.2021.200859","DOIUrl":"https://doi.org/10.21608/jbaar.2021.200859","url":null,"abstract":"Ahmed A. Yameny (Email: dr.ahmedyameny@yahoo.com) Tel: (002)01222708665, (002)01002112248 DOI: 10.21608/jbaar.2021.200859 Abstract: Background: Thrombocytopenia is a common manifestation and also an indicator of poor prognosis of SARS, MERS, and COVID-19 according to previous researches, Some studies have found a relationship between thrombocytopenia and the severity of the COVID-19 and related mortality. Patients and methods: This study included 504 out hospitalized patients with confirmed COVID-19 infection in Alexandria, Egypt, these study subjects were randomly selected irrespective of the age group and both genders were included, EDTA blood sample was collected for performing complete blood count and platelet count (Diagon D-cell 60 hematology analyzer Europe-Diagon Ltd. Hungary). Results: The present study included patients aged from14 years to 75 years mean age was 44.5 ±30.5 who were confirmed to have Covid-19 based on real-time reverse transcriptionpolymerase chain reaction, the female gender was more frequent (n=280, 55.6%) than Male gender (n=224, 44.4%). This study reveals a normal platelet count in 456 patients (90.5%), and a mild low platelet count of 140150× 10/L in 48 patients(9.5%), with a p-value, is 0.415 which is more than 0.05 not significant. And no patients in this studied group recorded platelet count less than 140× 10/L. Conclusion: Platelet was not a significant biomarker for COVID-19 diagnosis or prognosis in out-hospitalized patients (Outpatients and patients under home observation).","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75422320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-29DOI: 10.21608/jbaar.2021.198735
Abeer S. El-Maghraby, Yasser B. M. Ali, Eman A. El‐maadawy, M. Elshal, I. Bassyouni, I. El-Garawani, R. Talaat
Zoology Department, Faculty of Science, Menoufia University, Shebin El-Kom 32511, Menoufia, Egypt; dr.garawani@science.menofia.edu.eg; Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.dr_abeer_elmaghraby@yahoo.com, eman.anwr@gebri.usc.edu.eg, yasser.ali@gebri.usc.edu.eg, mohamed.elshal@gebri.usc.edu.eg Rheumatology and Rehabilitation Department, Faculty of Medicine, Cairo University, Cairo, Egypt.iman.bassyouni@kasralainy.edu.eg Corresponding author: Dr. Roba M. Talaat. Prof. of Molecular Immunology, Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City (USC), Egypt. Fax: +2 048 26
Menoufia大学理学院动物学系，Shebin El-Kom 32511，埃及Menoufia;dr.garawani@science.menofia.edu.eg;萨达特市大学遗传工程与生物技术研究所(GEBRI)分子生物系，Egypt.dr_abeer_elmaghraby@yahoo.com, eman.anwr@gebri.usc.edu.eg, yasser.ali@gebri.usc.edu.eg, mohamed.elshal@gebri.usc.edu.eg开罗大学医学院风湿病与康复系，开罗，Egypt.iman.bassyouni@kasralainy.edu.eg通讯作者:Roba M. Talaat博士。埃及萨达特城大学(USC)基因工程与生物技术研究所(GEBRI)分子生物系分子免疫学教授。传真:+ 204826
{"title":"T-lymphocyte subsets (CD3+, CD4+, and CD8+) in Systemic Lupus Erythematosus (SLE): Correlation with Clinical Manifestation","authors":"Abeer S. El-Maghraby, Yasser B. M. Ali, Eman A. El‐maadawy, M. Elshal, I. Bassyouni, I. El-Garawani, R. Talaat","doi":"10.21608/jbaar.2021.198735","DOIUrl":"https://doi.org/10.21608/jbaar.2021.198735","url":null,"abstract":"Zoology Department, Faculty of Science, Menoufia University, Shebin El-Kom 32511, Menoufia, Egypt; dr.garawani@science.menofia.edu.eg; Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.dr_abeer_elmaghraby@yahoo.com, eman.anwr@gebri.usc.edu.eg, yasser.ali@gebri.usc.edu.eg, mohamed.elshal@gebri.usc.edu.eg Rheumatology and Rehabilitation Department, Faculty of Medicine, Cairo University, Cairo, Egypt.iman.bassyouni@kasralainy.edu.eg Corresponding author: Dr. Roba M. Talaat. Prof. of Molecular Immunology, Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City (USC), Egypt. Fax: +2 048 26","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80421879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-28DOI: 10.21608/jbaar.2021.198090
M. Fouda, Sara A Mekkawy, Mariam Ghabour, Radwa Othman, Nayera Ahmed, Nour Habbib, Salsabeel Elkholey, R. Soliman, M. Fouad, Ellen Ayad, Mayar Shaqran, Mariam Mohamed, Rokaia M Aljarwani, Khaled Aboul-Enein, M. Omran
Globally, colorectal cancer (CRC) is one of the most often diagnosed solid tumors, with a significant death and morbidity rate. CRC biomarkers are desperately needed for early detection. Traditional CRC tumor markers do not have the best diagnostic performance. The levels of leptin and vitamin D were evaluated. CRC patients before treatment (n=16), CRC patients after treatment (n=14), and 20 patients with benign tumors were included in this case-control study. ELISA was used to determine the levels of traditional tumor markers (CA19.9 and CEA) as well as candidate markers (leptin and vitamin D). Using area receiver-operating characteristic analysis (AUC), the diagnostic performance of single and combination markers was assessed (ROC). The levels of CEA and CA 19.9 in the three groups studied were not significantly different. Vitamin D and leptin were significantly decreased (p= 0.03 and p= 0.02; respectively) in CRC patients than after benign patients. A novel combination, based on the combination of vitamin D and leptin was developed for CRC diagnosis using stepwise multivariate discriminant analysis (MDA). The combination can be represented as = (4.65 – vitamin D ((ng/ml)) × 0.009 + Leptin (ng/ml) × 0.441). AUCs were reported when leptin was used as a single biomarker for distinguishing CRC from benign (0.78) and non-treated CRC from treated CRC (0.67). When leptin and vitamin D were combined, the AUCs increased to 0.84 and0.72, respectively. Conclusion: Leptin and vitamin D were shown to be promising diagnostic and follow-up indicators for CRC in our investigation.
{"title":"Evaluation of diagnostic performances of leptin and vitamin D for colorectal cancer diagnosis and follow-up","authors":"M. Fouda, Sara A Mekkawy, Mariam Ghabour, Radwa Othman, Nayera Ahmed, Nour Habbib, Salsabeel Elkholey, R. Soliman, M. Fouad, Ellen Ayad, Mayar Shaqran, Mariam Mohamed, Rokaia M Aljarwani, Khaled Aboul-Enein, M. Omran","doi":"10.21608/jbaar.2021.198090","DOIUrl":"https://doi.org/10.21608/jbaar.2021.198090","url":null,"abstract":"Globally, colorectal cancer (CRC) is one of the most often diagnosed solid tumors, with a significant death and morbidity rate. CRC biomarkers are desperately needed for early detection. Traditional CRC tumor markers do not have the best diagnostic performance. The levels of leptin and vitamin D were evaluated. CRC patients before treatment (n=16), CRC patients after treatment (n=14), and 20 patients with benign tumors were included in this case-control study. ELISA was used to determine the levels of traditional tumor markers (CA19.9 and CEA) as well as candidate markers (leptin and vitamin D). Using area receiver-operating characteristic analysis (AUC), the diagnostic performance of single and combination markers was assessed (ROC). The levels of CEA and CA 19.9 in the three groups studied were not significantly different. Vitamin D and leptin were significantly decreased (p= 0.03 and p= 0.02; respectively) in CRC patients than after benign patients. A novel combination, based on the combination of vitamin D and leptin was developed for CRC diagnosis using stepwise multivariate discriminant analysis (MDA). The combination can be represented as = (4.65 – vitamin D ((ng/ml)) × 0.009 + Leptin (ng/ml) × 0.441). AUCs were reported when leptin was used as a single biomarker for distinguishing CRC from benign (0.78) and non-treated CRC from treated CRC (0.67). When leptin and vitamin D were combined, the AUCs increased to 0.84 and0.72, respectively. Conclusion: Leptin and vitamin D were shown to be promising diagnostic and follow-up indicators for CRC in our investigation.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87006647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：