Journal of Cancer Science & Therapy最新文献

英文中文

The Effects of Synthesized Rhenium Acetylsalicylate Compounds on Human Astrocytoma Cell Lines. 合成乙酰水杨酸铼化合物对人星形细胞瘤细胞系的影响。

Journal of Cancer Science & Therapy

Pub Date : 2018-01-01 Epub Date: 2018-02-26 DOI: 10.4172/1948-5956.1000512

Hirendra N Banerjee, Deidre Vaughan, Ava Boston, Gabriel Thorne, Gloria Payne, Josiah Sampson, Vinod Manglik, Pola Olczak, Brent V Powell, Angela Winstead, Roosevelt Shaw, Santosh K Mandal

Purpose: Because of the scarcity of suitable brain cancer drugs, researchers are frantically trying to discover novel and highly potent drugs free of side effects and drug-resistance. Rhenium compounds are known to be nontoxic and exhibit no drug resistance. For that reason, we have developed a series of novel rhenium acetylsalicylato (RAC or ASP) complexes to test their cytotoxicity on brain cancer cells. Also we have attempted to explore the DNAbinding properties of these compounds because many drugs either directly or indirectly bind to DNA.

Methods: We have treated the RAC series compounds on human astrocytoma brain cancer cell lines and rat normal brain astrocyte cells and determined the efficacy of these complexes through in vitro cytotoxicity assay. We carried out the DNA-binding study through UV titrations of a RAC compound with DNA. Also we attempted to determine the planarity of the polypyridyl ligands of the RAC series compounds using DFT calculations.

Results: RAC6 is more potent than any other RAC series compounds on HTB-12 human astrocytoma cancer cells as well as on Glioblastoma Multiforme D54 cell lines. In fact, The IC-50 value of RAC6 on HTB-12 cancer cells is approximately 2 μM. As expected, the RAC series compounds were not active on normal cells. The DFT calculations on the RAC series compounds were done and suggest that the polypyridyl ligands in the complexes are planar. The UV-titrations of RAC9 with DNA were carried out. It suggests that RAC9 and possibly all RAC series compounds bind to minor grooves of the DNA.

Conclusion: Because of the very low activity of RAC6 on normal cells and low lC₅₀ value of on astrocytoma (HTB-12) cell lines, it is possible that RAC6 and its derivatives may potentially find application in the treatment of brain cancers. The DFT calculations and UV titrations suggest that RAC series compounds either bind to DNA intercalatively or minor grooves of the DNA or both. However, it is highly premature to make any definite statement in the absence of other techniques.

目的:由于合适的脑癌药物的稀缺，研究人员正疯狂地试图发现新的和高效的药物，无副作用和耐药。铼化合物是无毒的，没有抗药性。因此，我们开发了一系列新的乙酰水杨酸铼(RAC或ASP)复合物来测试它们对脑癌细胞的细胞毒性。此外，由于许多药物直接或间接与DNA结合，我们也试图探索这些化合物的DNA结合特性。方法:将RAC系列化合物分别作用于人星形细胞瘤脑癌细胞株和大鼠正常脑星形细胞，并通过体外细胞毒实验测定其作用效果。我们通过紫外滴定RAC化合物与DNA进行了DNA结合研究。我们还尝试用DFT计算来确定RAC系列化合物的多吡啶配体的平面度。结果:RAC6对HTB-12人星形细胞瘤癌细胞和多形性胶质母细胞瘤D54细胞系的作用强于其他任何RAC系列化合物。事实上，RAC6对HTB-12癌细胞的IC-50值约为2 μM。正如预期的那样，RAC系列化合物对正常细胞没有活性。对RAC系列化合物进行了DFT计算，结果表明配合物中的多吡啶基配体是平面的。用DNA对RAC9进行紫外滴定。这表明RAC9和可能所有RAC系列化合物与DNA的小凹槽结合。结论:由于RAC6对正常细胞的活性很低，对星形细胞瘤(HTB-12)细胞株的lC50值很低，RAC6及其衍生物可能在脑癌的治疗中有潜在的应用前景。DFT计算和紫外滴定表明，RAC系列化合物要么与DNA插入性结合，要么与DNA的小凹槽结合，要么两者兼而有之。然而，在缺乏其他技术的情况下，做出任何明确的声明都是非常不成熟的。

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引用次数: 4

A Curious Umbilical Fistula: An Unexpected Onset of Urachal Mucinous Cystic Tumour 一个奇怪的脐瘘:一个意外发作的尿管粘液囊性肿瘤

Journal of Cancer Science & Therapy

Pub Date : 2018-01-01 DOI: 10.4172/1948-5956.1000518

F. Petrelli, R. Rossi, M. Fianchini, L. Cardinali, D. Marrelli, C. Marmorale

Background: The urachus is an embryonic remnant, which is obliterated in most of the adults. Residual of it persists in 32% of adults and neoplasms, which can arise from this structure, are extremely rare. These are usually diagnosed through incidental findings or for urinary symptoms. Their natural history is characterized by an early metastatization through the peritoneal cavity. Due to their rarity, there are no unanimous consensus for their management and therapy. Radical excision, associated or not with intraperitoneal hypertermic chemotherapy linked to cytoreductive surgery, was suggested over time.Case report: A black woman came to our attention for a gelatinous secretion from the umbilicus. After a MRIstudy, a parietal neoformation of the urachal remnants was diagnosed. This neoformation revealed a cutaneous fistulization and it was excised surgically. Its pathologic exam described the presence of a cystic mucinous urachal tumor. During the follow-up, a cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) was performed, due to the occurence of free intraperitoneal liquid and multiple nodules on the abdominal parenchimas, After the last surgery, the patient underwent radiological and serological follow up and, after 6 months, there are no evidence of pathological recurrence.Discussion and conclusion: In the diagnostic pathway, the MRI has a pivotal role for the study of the peritoneal cavity. Surgically, the complete excision is preferred. The role of the conventional chemotherapy is still under debate, however CRS in association with HIPEC, is certainly useful.

背景:urachus是胚胎的残余，在大多数成虫中是消失的。在32%的成年人中，它的残留仍然存在，而由这种结构引起的肿瘤极为罕见。这些通常是通过偶然发现或泌尿系统症状诊断出来的。其自然史的特点是通过腹膜腔早期转移。由于其罕见性，对其管理和治疗没有一致的共识。随着时间的推移，人们建议根治性切除，无论是否与与细胞减少手术相关的腹腔内高温化疗有关。病例报告:一名黑人妇女因脐部胶质分泌物引起我们的注意。mri检查后，诊断为尿路残余的顶叶新生物。该新生病变显示为皮肤瘘管化，手术切除。病理检查显示出现囊性粘液性尿管肿瘤。随访期间，因腹腔内游离积液及腹腔实质多发结节，行细胞减缩术(CRS)及腹腔内高热化疗(HIPEC)，术后行放射学及血清学随访，6个月后未见病理性复发。讨论与结论:在诊断途径中，MRI对腹膜腔的研究具有举足轻重的作用。手术上，完全切除是首选。传统化疗的作用仍在争论中，但与HIPEC相关的CRS肯定是有用的。

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引用次数: 0

A Rare Presentation of Neutrophilic Eccrine Hidradenitis in the Management of Acute Myeloid Leukaemia: A Case Report 急性髓性白血病治疗中罕见的中性粒细胞性汗腺炎一例报告

Journal of Cancer Science & Therapy

Pub Date : 2018-01-01 DOI: 10.4172/1948-5956.1000548

Nusrat Sanober, S. Talal, S. Ulahannan, J. Holter, A. Asch, M. Malla, Shannie Frisbie, S. Boddupalli

We present an unusual case of a 62-year-old male who was diagnosed with acute myeloid leukaemia and developed bilateral periocular skin dermatosis, concerning for neutrophilic eccrine hidradenitis, after receiving induction chemotherapy with Idarubicin and Cytarabine. On day seven of induction therapy, the patient developed swelling and discomfort around his right eye, which quickly progressed to involve the left side. Patient improved with supportive care with topical steroids and anti-inflammatory medications. Neutrophilic eccrine hidradenitis is typically a self-limited process. It does not appear to be associated with a worse prognosis for the underlying malignancy when occurring in that setting. The purpose of this report is to bring to light the importance of having a broad differential for dermal lesions in neutropenic patients and the avoidance of inappropriate therapy to prevent subsequent adverse events.

我们报告一个罕见的62岁男性病例，他被诊断为急性髓性白血病，并在接受伊达柔比星和阿糖胞苷诱导化疗后，发展为双侧眼周皮肤皮肤病，涉及中性粒细胞外分泌汗腺炎。在诱导治疗的第7天，患者右眼周围出现肿胀和不适，并迅速发展到左眼。患者在局部类固醇和抗炎药物的支持下得到改善。中性粒细胞分泌性汗腺炎是一种典型的自限性疾病。在这种情况下，它似乎与潜在恶性肿瘤的预后较差无关。本报告的目的是阐明对中性粒细胞减少患者皮肤病变进行广泛鉴别的重要性，以及避免不适当的治疗以防止随后的不良事件。

引用次数: 1

The Role of TGF-andbeta; in Gastrointestinal Cancers tgf -和β的作用胃肠道癌症

Journal of Cancer Science & Therapy

Pub Date : 2018-01-01 DOI: 10.4172/1948-5956.1000566

Hong Wang, Xiang Gong, Jingyu Xu, R. Xie

Transforming growth factor-beta (TGF-β) is a multifunctional cytokines of biologically active peptides. Recently years, the TGF-β was confirmed highly expressed in various types of human digestive system tumors. With the improvement of medical technology, the cure rate of early-stage tumor is obviously improved, but that of middleand advanced-stage tumors is still very low with poor prognosis. Therefore, it is important to explore a new targets, and increase the cure rate of malignant tumors and improve the life quality of patients. People have found that the tumor microenvironment (TME) can influence the tumors growth and evolution. The TME constituents such as pH, leukocytes, cytokines, extracellular matrix and humor, and contribute to the tumor cell proliferation, invasion and metastasis. There is plenty of evidence that TGF-β and their signaling play an important role in cell differentiation, inflammatory response, immunologic function and carcinogenesis in gastrointestinal cancers. The aims of this review is to provide a comprehensive view of TGF-β and its receptors and their function in the physiological and pathology mechanisms of the gastrointestinal cancers.

转化生长因子-β (TGF-β)是一种生物活性多肽的多功能细胞因子。近年来，TGF-β被证实在各种类型的人类消化系统肿瘤中高表达。随着医疗技术的进步，早期肿瘤的治愈率明显提高，但中晚期肿瘤的治愈率仍然很低，预后较差。因此，探索新的靶点，提高恶性肿瘤的治愈率，改善患者的生活质量具有重要意义。人们已经发现肿瘤微环境(tumor microenvironment, TME)可以影响肿瘤的生长和进化。TME成分如pH、白细胞、细胞因子、细胞外基质和幽默，参与肿瘤细胞的增殖、侵袭和转移。大量证据表明，TGF-β及其信号转导在胃肠道肿瘤的细胞分化、炎症反应、免疫功能和癌变过程中发挥重要作用。本文就TGF-β及其受体在胃肠道肿瘤的生理病理机制中的作用进行综述。

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引用次数: 2

Oculocutaneous Albinism in Pakistan: A Review 巴基斯坦皮肤白化病:综述

Journal of Cancer Science & Therapy

Pub Date : 2018-01-01 DOI: 10.4172/1948-5956.1000552

S. A. Shah, A. Saeed, M. Irshad, M. Babar, T. Hussain, A. Wajid, N. H. Memon, M. Idrees

Oculocutaneous albinism (OCA) is an autosomal recessive disorder of abnormal melanin biosynthesis characterized by hypopigmentation of skin, hair and eyes. The patients with OCA have high risk of skin cancer, actinic injury and nystagmus. Oculocutaneous albinism is further classified into non-syndromic OCA and syndromic OCA. Autosomal recessive disorders like oculocutaneous albinism are more common in Pakistani population due to cousin marriages and large consanguineous families. This review paper includes updated data on the different research work done in Pakistani population on the four types OCA1, OCA2, OCA3 and OCA4 of oculocutaneous albinism and the mutations reported, also little information about the new forms OCA5, OCA6 and OCA7of oculocutaneous albinism.

眼皮肤白化病(OCA)是一种常染色体隐性遗传病的异常黑色素生物合成的特点是皮肤，头发和眼睛色素沉着。OCA患者发生皮肤癌、光化损伤和眼球震颤的风险较高。眼皮肤白化病又分为非综合征性OCA和综合征性OCA。常染色体隐性遗传病，如皮肤白化病，在巴基斯坦人群中更为常见，因为近亲结婚和大的近亲家庭。本文综述了巴基斯坦人群中关于皮肤白化病OCA1、OCA2、OCA3和OCA4四种类型的不同研究工作及其突变报道的最新数据，以及关于皮肤白化病OCA5、OCA6和oca7新形式的信息。

引用次数: 1

Genes Correlated with Gemcitabine Efficacy in Non-small Cell Lung Cancer 与吉西他滨治疗非小细胞肺癌疗效相关的基因

Journal of Cancer Science & Therapy

Pub Date : 2018-01-01 DOI: 10.4172/1948-5956.1000540

Chunhong Li, Meiyan Liu, Hailing Lu, Wei Liu, L. Cai, Xiaoqun Dong

Objective: Gemcitabine in combination with platinum improves survival of patients with non-small cell lung cancer (NSCLC). The purpose of the study was to explore genes related to gemcitabine efficacy. Methods: The sensitivity of NSCLC cell lines to anticancer drugs was tested via MTT assay. Gene expression analysis was performed by cDNA microarray, and qRT-PCR was used for verification of the microarray results on highly sensitive genes. Fluorouracil (5-Fu) was used as the negative control of gemcitabine. Results: Gemcitabine-related and fluorouracil-related genes were pooled into different clusters. Genes negatively related to 5-Fu sensitivity were positively related to gemcitabine efficacy. Metallothionein, Cathepsin B, TIMP1 and Galectin-1 genes which were resisted to certain anticancer drugs were sensitive to gemcitabine (P<0.05). Conclusion: Metallothionein, Cathepsin B, TIMP1 and Galectin-1 can be considered as the predictors for gemcitabine sensitivity.

目的:吉西他滨联合铂可提高非小细胞肺癌(NSCLC)患者的生存率。该研究的目的是探索与吉西他滨疗效相关的基因。方法:采用MTT法检测非小细胞肺癌细胞株对抗癌药物的敏感性。基因表达分析采用cDNA芯片，高敏感基因采用qRT-PCR对芯片结果进行验证。以氟尿嘧啶(5-Fu)作为吉西他滨阴性对照。结果:吉西他滨相关基因和氟尿嘧啶相关基因汇集成不同的簇。与5-Fu敏感性负相关的基因与吉西他滨疗效正相关。对部分抗癌药物耐药的金属硫蛋白、组织蛋白酶B、TIMP1、半凝集素-1基因对吉西他滨敏感(P<0.05)。结论:金属硫蛋白、组织蛋白酶B、TIMP1和半凝集素-1可作为吉西他滨敏感性的预测因子。

引用次数: 1

Zoledronate Enhances the Cytotoxicity of Gamma Delta T Cell Immunotherapy in an Orthotopic Mouse Model of Osteolytic Osteosarcoma 唑来膦酸钠增强γ δ T细胞免疫治疗对骨溶解性骨肉瘤小鼠模型的细胞毒性

Journal of Cancer Science & Therapy

Pub Date : 2018-01-01 DOI: 10.4172/1948-5956.1000554

A. Zysk, M. DeNichilo, Irene Zinonos, S. Hay, Vasilios Liapis, V. Ponomarev, A. Zannettino, A. Evdokiou, V. Panagopoulos

Objective: Osteosarcoma is the most common primary tumor of the bone, predominantly affecting children and adolescents. While localized osteosarcoma can be readily treated with the use of pre-operative chemotherapy in combination with surgery, patients who develop metastatic disease and tumor-induced osteolysis continue to have a poor prognosis. Many cancer cells express tumor-specific antigens, rendering them vulnerable to immune effector T cell killing. There is increasing evidence that highly cytotoxic gamma delta (Vγ9Vδ2) T cells together with the bone anti-resorptive drug zoledronate may hold significant clinical benefit in the treatment of a variety of tumor types. Methods: Ex vivo expanded Vγ9Vδ2 T cells were used to assess effector-mediated killing of osteosarcoma cells (BTK-143 and K-HOS) in response to zoledronate pre-treatment. An orthotopic mouse model of osteolytic osteosarcoma was used to verify Vγ9Vδ2 T cell cytotoxicity in combination with zoledronate on tumor growth, osteolysis and metastasis. Results: Pre-treatment of osteosarcoma cells with zoledronate enhanced Vγ9Vδ2 T cell rapid killing compared to untreated cells in vitro via blockade of the mevalonate pathway. When adoptively transferred into osteosarcoma bearing NOD/SCID mice in vivo, Vγ9Vδ2 T cells in combination with zoledronate potentiated the anti-cancer efficacy of Vγ9Vδ2T cells and inhibited tumor induced osteolysis. Importantly, Vγ9Vδ2 T cells alone reduced both the incidence and burden of lung metastases. Conclusion: This study demonstrated the dual-action of zoledronate to enhance the immunogenicity of osteosarcoma cells to Vγ9Vδ2 T cell cytotoxicity and provide protection against tumor-induced osteolysis.

目的:骨肉瘤是最常见的骨原发肿瘤，主要影响儿童和青少年。虽然局部骨肉瘤可以很容易地通过术前化疗结合手术治疗，但发生转移性疾病和肿瘤诱导的骨溶解的患者预后仍然很差。许多癌细胞表达肿瘤特异性抗原，使它们容易受到免疫效应T细胞的杀伤。越来越多的证据表明，高细胞毒性γ δ (v γ γ 9v δ2) T细胞与骨抗吸收药物唑来膦酸盐一起治疗多种肿瘤类型可能具有显著的临床疗效。方法:利用体外扩增的Vγ9Vδ2 T细胞，评估效应介导的骨肉瘤细胞(BTK-143和K-HOS)对唑来膦酸钠预处理的杀伤作用。采用原位小鼠骨溶解性骨肉瘤模型，验证了唑来膦酸钠联合使用v γ - 9v - δ2 T细胞对肿瘤生长、骨溶解和转移的毒性作用。结果:与未处理骨肉瘤细胞相比，用唑来膦酸钠预处理骨肉瘤细胞通过阻断甲羟戊酸途径增强了Vγ9Vδ2 T细胞的体外快速杀伤。将Vγ9Vδ2T细胞在体内过继转移至NOD/SCID小鼠骨肉瘤后，与唑来膦酸钠联合使用可增强Vγ9Vδ2T细胞的抗癌作用，抑制肿瘤诱导的骨溶解。重要的是，单独使用Vγ9Vδ2 T细胞可以降低肺转移的发生率和负担。结论:唑来膦酸钠具有增强骨肉瘤细胞免疫原性的双重作用，对v - γ - 9v - δ2 T细胞毒性具有保护作用。

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引用次数: 0

Evaluation of the Effect of Lactobacillus planetarium Probiotics Produced from Broad Bean Seed in Prevention of Helicobacter pylori in Stomach Tissue of C57BL/6 Mice 蚕豆种子产行星乳杆菌益生菌对C57BL/6小鼠胃组织幽门螺杆菌预防作用的评价

Journal of Cancer Science & Therapy

Pub Date : 2018-01-01 DOI: 10.4172/1948-5956.1000524

A. Afsahi, Hassan Mahmoudi, A. Ebrahimi, Z. Aeini, D. Esmaeili

Background and aims: Helicobacter pylori is one of the most common human infections, which colonizes more than half of the world’s population. This causes chronic stomach inflammation diseases without clinical syndromes, gastric and duodenal ulcer, and stomach cancer. Nowadays, the use of probiotics has received much consideration as one of the common therapeutic methods, which prevents bacterial colonization by creating a balance in the microbial gastrointestinal tract.Methods: This experimental study was conducted on 30 rats in five groups from August 2016 to June 2017 in the Microbiology and Animal Laboratory of Shahrekord University. First, the rats were infected with H. pylori bacteria. PCR method was used to confirm the presence of bacteria in the stomach to ensure that the rats were inoculated with H. pylori. After inoculation, the infected rats were treated with probiotic product, and then gastric tissue of the infected group was evaluated by haematoxylin and eosin stain.Results: The absence of Cag A and Ure C genes in fecal specimens of the group receiving probiotic products before and after H. pylori incubation showed a positive effect for this product on the prevention and treatment of H. pylori infection. Also, in stomach histology specimens, the effects of mild inflammation were observed in treated group with the probiotic product before and after H. pylori inoculation compared to the control group.Conclusion: The results of this study showed that the addition of probiotic to a non-dairy product (broad bean extract) can be effective in preventing and treating H. pylori infection in the animal model.

背景和目的:幽门螺杆菌是最常见的人类感染之一，世界上一半以上的人口都有幽门螺杆菌。导致无临床证候的慢性胃炎疾病、胃和十二指肠溃疡、胃癌。如今，使用益生菌作为一种常见的治疗方法已经得到了广泛的考虑，它通过在微生物胃肠道中创造平衡来防止细菌定植。方法:本实验研究于2016年8月至2017年6月在Shahrekord大学微生物与动物实验室分5组30只大鼠进行。首先，老鼠感染了幽门螺杆菌。采用PCR法确认胃内细菌的存在，确保大鼠接种了幽门螺杆菌。接种后用益生菌制剂对感染大鼠进行处理，然后用苏木精和伊红染色对感染组胃组织进行评价。结果:在幽门螺杆菌孵育前后接受益生菌产品组的粪便标本中，Cag A和Ure C基因缺失，表明该产品对预防和治疗幽门螺杆菌感染有积极作用。同时，在胃组织学标本中，与对照组相比，接种幽门螺杆菌前后使用益生菌产品治疗组对轻度炎症的影响。结论:本研究结果表明，在非乳制品(蚕豆提取物)中添加益生菌可有效预防和治疗动物模型的幽门螺杆菌感染。

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引用次数: 5

A Case Report on the Right Cervical Lymph Node Metastasis of Hepatocellular Carcinoma 肝细胞癌右颈淋巴结转移1例报告

Journal of Cancer Science & Therapy

Pub Date : 2018-01-01 DOI: 10.4172/1948-5956.1000563

Zheng-Jun Zhao, J. Maia, Xin-rui Zhang, Honghong Yan, Xiaoqi Chen, Xuekui Liu

引用次数: 2

Advances in NKT cell Immunotherapy for Glioblastoma. NKT细胞免疫治疗胶质母细胞瘤的研究进展。

Journal of Cancer Science & Therapy

Pub Date : 2018-01-01 Epub Date: 2018-06-18 DOI: 10.4172/1948-5956.1000533

Kalyani Pyaram, Viveka Nand Yadav

Type I or invariant natural killer T cells belong to a unique lineage of innate T cells, which express markers of both T lymphocytes and NK cells, namely T cell receptor (TCR) and NK1.1 (CD161C), respectively. Thus, apart from direct killing of target cells like NK cells, and they also produce a myriad of cytokines which modulate the adaptive immune responses. Unlike traditional T cells which carry a conventional αβ TCR, NKT cells express semi-invariant TCR - Vα14-Jα18, coupled with Vβ8, Vβ7 and Vβ2 in mice. In humans, the invariant TCR is composed of Vα24-Jα18, coupled with Vβ11.

I型或不变自然杀伤T细胞属于一种独特的先天T细胞谱系，它表达T淋巴细胞和NK细胞的标记物，即T细胞受体(TCR)和NK1.1 (CD161C)。因此，除了直接杀伤NK细胞等靶细胞外，它们还产生无数调节适应性免疫反应的细胞因子。与传统的携带αβ TCR的T细胞不同，NKT细胞在小鼠中表达半不变的TCR - Vα14-Jα18，偶联Vβ8、Vβ7和Vβ2。在人类中，不变的TCR由Vα24-Jα18与Vβ11偶联组成。

引用次数: 6

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