Journal of Biomaterials Science, Polymer Edition最新文献

Spinal cord injury (SCI) is one of the most complex diseases. After SCI, severe secondary injuries can cause intense inflammatory storms and oxidative stress responses, leading to extensive neuronal apoptosis. Effective regulation of inflammation and oxidative stress after SCI remains an unresolved challenge. In this study, resveratrol-loaded nanoparticles coated with neutrophil membranes (NMR) were prepared using the emulsion-solvent evaporation method and membrane encapsulation technology. Multifunctional biomimetic nanoparticles retain neutrophil membrane-related receptors and possess a strong adsorption capacity for inflammatory factors. As a drug carrier, NMR can sustainably release resveratrol for >72 h. Moreover, co-culture studies in vitro show that the NMR help regulate macrophage polarization to relieve inflammatory response, reduce intracellular reactive oxygen species by approximately 50%, and improve mitochondrial membrane potential to alleviate oxidative stress. After injecting NMR into the injury site, it reduces early apoptosis, inhibit scar formation, and promote neural network recovery to improve motor function. This study demonstrates the anti-inflammatory, antioxidant, and neuroprotective effects of NMR, thus providing a novel therapeutic strategy for SCI.

The aim of this study is to investigate the impact of sh-LncRNA ASB16-AS1 on doxorubicin (DOX) resistance in colorectal cancer (CRC). First, an in vitro study was conducted to investigate the effects of LncRNA ASB16-AS1, miR-185-5p, and TEAD1 on drug resistance in CRC cells. Subsequently, utilizing nanotechnology, poly(beta amino esters) (PBAE)/zeolitic imidazolate framework-8 (ZIF-8)@sh-LncRNA ASB16-AS1 nanoparticles (PZSNP) were synthesized and characterized, evaluating their cellular toxicity and hemolytic activity. Finally, a mouse subcutaneous tumor model was established by subcutaneous injection of SW480/DOX cell suspension to investigate the impact of PZSNP on the tumor. Under DOX treatment, downregulation of LncRNA ASB16-AS1, overexpression of miR-185-5p, or downregulation of TEAD1 suppressed the viability and proliferation of drug-resistant CRC cells while promoting apoptosis. Conversely, overexpression of LncRNA ASB16-AS1, inhibition of miR-185-5p, or overexpression of TEAD1 enhanced the viability and proliferation of drug-resistant CRC cells while inhibiting apoptosis. The synthesized PZSNP exhibited a spherical shape with an average particle size of 123.6 nm, possessed positive charge, displayed good stability. It effectively encapsulated shRNA and displayed low cellular toxicity and hemolytic activity. Under DOX treatment, significant tumor necrosis was observed in the PZSNP group, and tumor growth was suppressed without causing weight loss. LncRNA ASB16-AS1, miR-185-5p, and TEAD1 are involved in regulating cell viability, proliferation, and apoptosis, contributing to drug resistance in CRC cells. sh-LncRNA ASB16-AS1 enhances the sensitivity of CRC cells to DOX during treatment, and in vivo delivery of PZSNP may serve as an effective strategy to overcome chemotherapy resistance in CRC.

Diabetic retinopathy (DR) is a common microvascular complication of diabetes necessitating early intervention to impede progression, despite current clinical treatments focusing on advanced stages. Essential oils from Fructus Alpiniae zerumbet (EOFAZ) have demonstrated efficacy in protecting against high glucose (HG)-induced Müller cell activation and DR development. This study introduced a reactive oxidative species (ROS)-responsive drug delivery system (NPS_PHE@EOFAZ) targeting early DR stages and oxidative stress. Our engineered nanoparticles effectively deliver EOFAZ into HG-exposed Müller cells by detecting and responding to elevated oxidative stress levels. The NPS_PHE@EOFAZ significantly inhibited abnormal cell growth, reduced oxidative stress, and alleviated inflammation in vitro. In vivo experiments on diabetic mice with DR revealed that NPS_PHE@EOFAZ mitigated early pathological changes by reducing oxidative stress and inflammation while also alleviating organ damage in the heart, liver, spleen, lung, and kidney. These findings underscore the potential of NPS_PHE@EOFAZ as a promising antioxidant for early intervention in DR pathogenesis.

There have been studies published on the composition and coating uses of raw lacquers following enzymatic oxidative polymerization. The change of urushiol' thermal stability and biological activity following polymerization to create oligomer, however, has received little attention. This work using silica gel column chromatography to separate urushiol and urushiol oligomer from polymerized raw lacquer and assessed its antibacterial, antioxidant, and thermal stability in an effort to decrease the allergenicity of urushiol and increase its application. By using gel chromatography, the urushiol oligomer were discovered to be polymers with 2-5 degrees of polymerization. According to characterization results from techniques like UV, FT-IR, and ¹H NMR, urushiol was converted into urushiol oligomer by addition reactions, and C-C coupling. The findings demonstrated that the urushiol oligomer' IC₅₀ values for scavenging DPPH and ABTS free radicals were 40.8 and 27.4 μg/mL, respectively, and that their minimum inhibitory concentrations against Staphylococcus aureus and Staphylococcus epidermidis were 250 and 125 μg/mL. The urushiol oligomer's thermogravimetric differential curve peak temperature (461.8 °C) was higher than urushiol's (239.5 °C), indicating that urushiol undergoes polymerization with enhanced thermal stability. The study's findings establish a foundation for the use of polymerized urushiol and urushiol oligomer in applications including functional materials and additives.

Emphasizing the viscoelasticity of ophthalmic gels is crucial for understanding the residence time, structure, and stability of hydrogels. This study primarily aimed to propose an innovative rheology analysis method for ophthalmic gels, considering complex eye movements. This method was applied to select ophthalmic gels with favorable rheological characteristics. Additionally, the physical characteristics and in vitro release of the selected Panax notoginseng total saponins (PNS) gel were demonstrated. The selected PNS gel significantly increased the activities of SOD and decreased intracellular levels of MDA, TNF-α, and IL-1β in H₂O₂-treated ARPE-19 cells. Finally, the optimal formulation was selected as a suitable platform for ophthalmic delivery and was shown to significantly rescue ARPE-19 cells from oxidative cellular damage.

The treatment of degenerative pathologies affecting articular cartilage remains a significant clinical challenge. Non-invasive biophysical stimuli, such as electric fields, have demonstrated potential as therapeutic tools for cartilage tissue restoration. Previous studies have reported that electric fields enhance chondrocyte proliferation and the synthesis of key extracellular matrix components, such as glycosaminoglycans. However, inconsistencies in experimental designs have led to variable findings. This study examines the effects of capacitively coupled electric fields on chondrocytes cultured in gelatin hydrogels. Alternating voltages of 50 V (7.7 mV/cm) and 100 V (8.7 mV/cm) at a frequency of 60 kHz were applied for 21 days. Cell quantification and glycosaminoglycan analysis were performed on both stimulated and control samples. On day 7, exposure to the electric field resulted in a significant reduction in cell proliferation by 24.7% and 39.2% at 7.7 mV/cm and 8.7 mV/cm, respectively (p < 0.05). However, stimulation at 8.7 mV/cm led to a 35.7% increase in glycosaminoglycan synthesis compared to the control group (p < 0.05). These findings indicate that electric field stimulation can modulate the synthesis of essential extracellular matrix components, such as glycosaminoglycans, in hyaline cartilage. This highlights the potential of electric fields as a promising strategy to enhance outcomes in articular cartilage tissue engineering, particularly in hydrogel-based therapeutic approaches.

The study examined the potential applications of propolis, polyvinyl alcohol (PVA), and Alhagi maurorum extracts in drug delivery systems, utilizing both computer and lab methods. The study uses molecular docking probes along with DFT (density functional theory) to investigate molecular interactions and examine the binding of drugs to carrier materials. The HOMO (Highest Occupied Molecular Orbital)-LUMO (Lowest Occupied Molecular Orbital) gap for the mix of PVA, galangin, and triterpene glycoside is -0.07621 eV, which matches the experiment results. This small gap enhances responsiveness in drug delivery applications, which is crucial for successful interactions with biological targets. It's possible that a delivery system that combines galangin and triterpene glycosides would work better and be more compatible with living things.The experimental results of the Methyl Thiazole Tetrazolium (MTT) show consistent findings: The viability of MCF7, a human breast cancer cell line, significantly decreased at all concentrations of propolis and polyvinyl alcohol compared to WRL68, a fetal liver cell line. Within-group comparisons showed less viability in both groups at 400 µg/ml. Mean ± SD: 42.05267 ± 1.951655; 67.12533 ± 7.401263.In the positive control group, the average number of malignant cells was 47.06, but the average number of cells in the fourth treatment (Propolis + PVA) and the third combination (Propolis + Alhagi maurorum + PVA) were 42.05267 and 42.97800, respectively. The Sustainable Development Goals in Industry and Innovation are focusing on developing a new combination of alhagi and propolis using PVA as a polymer carrier.

The science of Bone tissue engineering (TE) is quickly progressing. Engineering bone usually applications a synthetic extracellular matrix, cells or osteoblasts that can convert to osteoblasts, and adjusting causes that boost adhesion, distinction, and mineralized bone construction of cells. Extremely porous scaffolds perform an important character in cell planting, propagation, and fresh 3D-tissue construction. Reformative medicine and tissue engineering track a multi-disciplinary approach for the novel substances' development and appliance, to the various tissue defects therapy. The presentation of polyhedral oligomeric silsesquioxane (POSS) in the bio-polymeric scaffold has been shown to develop the biotic attributes of the hybrid combinations. This review focuses on the influence of POSS within the Chitosan (CS), Hydroxyapatite (HA), and zeolite matrixes, scaffold drawing, and the advantages and limitations of the materials mentioned for tissue engineering of bone.

Infectious bone defects pose a significant challenge in orthopedics by hindering healing and vascularization. This study explored the impact of fibroin thermosensitive hydrogel on osteogenesis, inflammatory response, and angiogenesis as a potential biomaterial for bone regeneration in osteomyelitis treatment. The biocompatibility of the hydrogel by live/dead staining revealed a high number of viable osteoblast cells after 14 days. ALP activity was significantly increased in all hydrogel formulations, with F3 showing the highest levels of total protein content and calcium deposition, indicating more effective osteogenesis. Gene expression analysis of the osteogenesis-related genes demonstrated that RUNX2 was upregulated by day 7, followed by increased expressions of the OCN and COL-1 genes at later stages. The inflammatory response to F3 was assessed by measuring the nitric oxide (NO) production and pro-inflammatory gene expression in LPS-stimulated RAW 264.7 macrophages. The F3 formulation significantly reduced NO production and iNOS expression, suggesting selective inhibition of the inflammatory pathway. The VEGF-loaded F3 formulation exhibited substantial angiogenic potential, enhancing HUVEC cell proliferation by 140% over 48 h. The osteogenic, anti-inflammatory, and angiogenic effects shown by the F3 formulation were well-suited for applications in osteomyelitis treatment.

Cerebrospinal fluid (CSF) leakage caused by accidents or diseases resulting from traumatic brain injury, inflammation, tumor erosion and surgery can lead to many complications. In this study, a multifunctional composite double-layer hydrogel was designed by simulating the structure of native dura mater, which was composed of polyacrylic acid (PAA), polyethyleneimine (PEI), sodium alginate (SA), β-cyclodextrin (β-CD) and edaravone (Ed). The PAA/PEI layer had strong wet adhesion characteristics, while the PEI/SA@β-CD/Ed layer exhibited significant antioxidant, drug release and biocompatibility properties. By controlling the concentration of Ca²⁺, the gelation time can be adjusted rapidly within 95-215 s. Specifically, the final PAA/PEI/SA@β-CD/Ed composite hydrogel exhibited a porous network structure with high porosity and low swelling rate, improved tensile strength, sufficient biodegradability, favourable adhesion performance, enhanced DPPH and ABTS radicals scavenging abilities, and sustained Ed release capacity. In addition, the resulting hydrogel also showed excellent biocompatibility and protective effect on H₂O₂-induced oxidative damage in SH-SY5Y cells. These results preliminarily suggested that the PAA/PEI/SA@β-CD/Ed composite hydrogel would appear to be a promising candidate for dural repair.