This study aims to investigate the effects of zinc-fortified germination on gamma-aminobutyric acid (GABA) content, zinc concentration, antioxidant activities, and flavor characteristics in brown rice (BR). Germination was conducted at 30 °C for 28 h using a 30 mg/L zinc sulfate solution. Germinated brown rice (GBR) was selected as the control sample for determining all parameters. Combining germination with zinc fortification in BR enhanced GABA content and zinc accumulation, reaching their peak around 28 and 34 h of germination, respectively. Antioxidant assays evaluated potential health benefits. Compared to conventional germination, zinc-fortified germination significantly increased the antioxidant activities of BR as germination time increased. Volatile compounds were compared in BR, GBR, and zinc-fortified germination brown rice (Zn-GBR). Among the 20 volatile compounds identified, 8 showed significant differences. The combined germination and zinc fortification treatment effectively reduced the content of volatile organic compounds (VOCs) that contribute to undesirable flavors. Germination significantly increased two ketone compounds in BR, while zinc fortification added sweet, nutty, caramel, and fruity-like aromas. The results in this study indicated that combined germination and zinc fortification could effectively increase the GABA content, zinc accumulation, and antioxidant activities. It would be a promising method to enhance the flavor of BR.
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