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Nitrogen topdressing at panicle initiation modulated nitrogen allocation between storage proteins and free nitrogenous compounds in grains of japonica rice 粳稻谷粒中贮藏蛋白和游离氮化合物之间的氮分配调控
IF 3.9 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-01 DOI: 10.1016/j.jcs.2024.104010
Luqi Wang , Lei Yang , Luoluo Hu , Yanyan Ren , Ganghua Li , Yanfeng Ding , Matthew J. Paul , Zhenghui Liu

Nitrogen (N) topdressing often leads to a decline in cooking and eating quality due to alterations in grain chemical composition. Investigation into the response of N compounds in grains, such as protein-bound amino acids (PAA), free amino acids (FAA), and other residue N compounds (Nresidue), would be beneficial for understanding the impact of N on rice quality. The present study selected five japonica rice cultivars and conducted a two-year field experiment employing two N fertilization treatments: the CK treatment, where all N fertilizer was applied as basal fertilizer, and the topdressing treatment, where 50% of the total N was applied as topdressing at the panicle initiation stage. Averaging across years and cultivars, N topdressing resulted in a 16.3% increase in PAA and an 8.65% decrease in FAA, with no significant impact on Nresidue. N topdressing resulted in a decrease in the proportion of essential amino acids found in prolamins, as well as a reduction in the proportion of amino acids belonging to the aspartate family and arginine. Significant genotypic variations were observed in the response of nitrogen components to topdressing. Furthermore, elite cultivars with premium quality exhibited greater stability in amylose content compared to protein content.

由于谷物化学成分的改变,氮(N)的上层施用通常会导致烹饪和食用品质的下降。调查谷物中氮化合物的反应,如蛋白质结合氨基酸(PAA)、游离氨基酸(FAA)和其他残留氮化合物(Nresidue),将有助于了解氮对稻米品质的影响。本研究选取了五个粳稻栽培品种,进行了为期两年的田间试验,采用了两种氮肥处理方法:CK 处理(所有氮肥均作为基肥施用)和上施处理(总氮肥量的 50%作为上施氮肥在稻穗初生期施用)。从不同年份和品种的平均值来看,氮肥上施导致 PAA 增加了 16.3%,FAA 减少了 8.65%,而对 Nresidue 没有显著影响。氮上覆会导致脯氨酸中必需氨基酸比例的下降,以及属于天冬氨酸家族的氨基酸和精氨酸比例的下降。在氮成分对表层施肥的反应方面,观察到了显著的基因型差异。此外,与蛋白质含量相比,优质栽培品种的直链淀粉含量表现出更大的稳定性。
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引用次数: 0
Corrigendum to “Ultrasonication assisted pearl millet starch-germ complexing: Evaluation of starch characteristics and its influence on glycaemic index of bread” [J. Cereal. Sci. 112 (July 2023) 103686] 超声波辅助珍珠粟淀粉-胚芽复合:淀粉特性评估及其对面包血糖指数的影响" [J. Cereal.
IF 3.9 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-09-01 DOI: 10.1016/j.jcs.2024.104000
R. Vidhyalakshmi , Pichan Prabhasankar , M.S. Meera
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引用次数: 0
Breeding wheat for organic farming: Can the high grain protein gene Gpc-B1 help to tackle challenges in view of end-use quality? 为有机农业培育小麦:高谷粒蛋白基因 Gpc-B1 能否帮助应对最终使用质量方面的挑战?
IF 3.9 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-28 DOI: 10.1016/j.jcs.2024.104011
Heinrich Grausgruber , Hayusha Naresh Pancholi , Matilda Ciucă , Daniel Cristina , Marianna Mayer , Denisse Bender

Organic farming contributes to sustain healthy ecosystems, but challenges such as lower crop yields and supply of the nitrogen needs of crops remain. Wheat is the most important organic arable crop in Europe and grain protein content is the main quality trait also for grading organic wheat despite that high baking quality can be also realized at lower protein contents. Hence, breeding of organic wheat varieties that realize a stable high grain protein content even at lower nitrogen availability is of utmost importance to guarantee income of organic wheat growers. A major QTL for high grain protein content was identified in wild emmer and transferred into bread wheat. We tested six different wheat genetic backgrounds varying for the presence/absence of the functional Gpc-B1 allele in multi-location trials in Central Europe for their performance under organic growing. The increase in grain protein content caused by the functional Gpc-B1 allele was present in almost all genetic backgrounds, however, was not consistent across all tested quality traits. None the less, the functional Gpc-B1 allele may play a major role to increase the stability of organic wheat to reach minimum requirements by traders and processors with respect to grain protein content.

有机耕作有助于维持健康的生态系统,但作物产量较低和作物氮需求供应等挑战依然存在。小麦是欧洲最重要的有机耕地作物,谷物蛋白质含量也是有机小麦分级的主要质量性状,尽管较低的蛋白质含量也能实现较高的烘焙质量。因此,培育即使在氮含量较低的情况下也能实现稳定的高谷物蛋白质含量的有机小麦品种,对于保证有机小麦种植者的收入至关重要。我们在野生小麦中发现了一个主要的高谷物蛋白质含量 QTL,并将其转移到面包小麦中。我们在中欧的多地试验中测试了六种不同的小麦遗传背景,这些背景因存在/不存在功能性 Gpc-B1 等位基因而有所不同,以测试它们在有机种植条件下的表现。功能性 Gpc-B1 等位基因导致的谷物蛋白质含量增加几乎存在于所有遗传背景中,但在所有测试的质量性状中并不一致。尽管如此,功能性 Gpc-B1 等位基因可能在提高有机小麦的稳定性,使其达到贸易商和加工商对谷物蛋白质含量的最低要求方面发挥重要作用。
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引用次数: 0
The structural-mechanical damage behavior of rice bran layer under tensile processing for moderate milling 适度碾磨拉伸加工下米糠层的结构-机械损伤行为
IF 3.9 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-20 DOI: 10.1016/j.jcs.2024.104007
Zilong Xu , Liu Yang , Pingan Huang , Bo Cui , Can Shu , Yang Luo , Xuan Xiao , Shaoyun Song , Houchang Pei , Yonglin Zhang

Bran layer moderate removal during rice milling is a crucial process for reducing rice broken rate and nutrient loss. The bran layer structural-cracking behavior is analyzed using a self-developed in-situ observation tensile tester with high-speed image acquisition. The rice bran tissue microstructure, chemical composition, cracking morphology are in-depth investigated. The results show that rice bran pericarp cells are of reticulate network, cell shape with rectangular or elliptical.significant differences in cell distribution and density across rice varieties, with Japonica rice (NJ5055) with a denser and more uniform structure, exhibiting higher ductility and tensile strength. The rice bran exhibited linear elastic rheological behavior. The bran tensile property is different under tensing directions, the tensile strength shows longitudinal (Y) > radial (X). Cracking along the cell long axis is Y direction, showing orderly line cracking path. Cracking along cell short axis is X direction, showing zigzag cracking path. Pericarp cells geometrical structure determines the mechanical strength and cracking-removal resistance ability of rice bran. The pericarp layer significantly enhances the ductility of the bran layer, allowing it to stretch and deform under stress, thereby improving overall mechanical performance. Rice bran structural-mechanical properties are affected by micro-structure and components.

在碾米过程中适度去除糠层是降低碎米率和营养损失的关键工序。利用自主研发的高速图像采集原位观察拉伸试验机分析了米糠层的结构-开裂行为。深入研究了米糠组织的微观结构、化学成分和开裂形态。结果表明,米糠果皮细胞呈网状,细胞形状为矩形或椭圆形,不同水稻品种的细胞分布和密度存在显著差异,粳稻(NJ5055)的结构更致密、更均匀,表现出更高的延展性和抗拉强度。米糠表现出线性弹性流变行为。米糠的拉伸特性在拉伸方向上有所不同,拉伸强度表现为纵向(Y)> 径向(X)。沿细胞长轴的开裂方向为 Y 向,呈现有序的线状开裂路径。沿细胞短轴的裂纹为 X 向,呈 "之 "字形裂纹。果皮细胞的几何结构决定了米糠的机械强度和抗开裂能力。果皮层可明显增强米糠层的延展性,使其在应力作用下伸展变形,从而改善整体机械性能。米糠的结构机械性能受到微观结构和成分的影响。
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引用次数: 0
Quality and microstructural analysis of chickpea-enriched wheat semolina pasta using X-ray micro-computed tomography 利用 X 射线显微计算机断层扫描技术分析富含鹰嘴豆的小麦粗面的质量和微观结构
IF 3.9 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-20 DOI: 10.1016/j.jcs.2024.104009
Obasi Ukpai Ukoji , Muhammad Mudassir Arif Chaudhry , Lindsey Boyd , Elaine Sopiwnyk , Jitendra Paliwal

The microstructure of manufactured foods largely governs palatability qualities such as crunchiness, crispness, mouthfeel and texture. While enriching cereal flour with leguminous flour increases its nutritional value, it should not compromise the palatability of the end products. This study investigated the flour blend properties as well as the physical and microstructural characteristics of durum wheat semolina pasta enriched with 50% chickpea flour obtained from single-stage (Ferkar) and multi-stage (roller) mills. Results of the flour blend analysis indicated the influence of milling on the protein, ash and particle size distribution of pasta. High protein chickpea blend showed minimal impact on the microstructure of the pasta. Also, the textural analysis of the pasta samples showed that the hardness and firmness of pasta made with roller-milled flours, specifically reduction and straight-grade flours, were not statistically significantly different (p < 0.05). However, correlating the texture and total porosity indicated that flour from roller mill reduction rolls resulted in pasta with a firmer texture along with a more compact internal and external microstructure. The findings of this study provide useful insight into the quality attributes of chickpea-enriched wheat semolina pasta as influenced by the mill type and stream blend of the chickpea flour.

人造食品的微观结构在很大程度上决定了其口感质量,如松脆度、脆度、口感和质地。在谷物面粉中添加豆科植物面粉可提高其营养价值,但不应影响最终产品的适口性。本研究调查了通过单级(费卡)和多级(辊筒)磨粉机获得的富含 50%鹰嘴豆面粉的硬质小麦粉面条的混合面粉特性以及物理和微观结构特征。混合面粉的分析结果表明,研磨对意大利面的蛋白质、灰分和粒度分布有影响。高蛋白鹰嘴豆混合粉对意大利面微观结构的影响最小。此外,面食样品的质地分析表明,用辊磨面粉(特别是还原级面粉和直级面粉)制成的面食的硬度和紧实度在统计学上没有显著差异(p <0.05)。然而,质地和总孔隙率的相关性表明,辊磨还原辊面粉制成的意大利面质地更硬,内部和外部微观结构更紧凑。这项研究的结果为了解鹰嘴豆富集小麦粉面食的质量属性受磨粉机类型和鹰嘴豆面粉混合流的影响提供了有益的启示。
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引用次数: 0
Effect of ultrasound combined with exogenous protein treatment on the flushing characteristics of puffed corn flour 超声波结合外源蛋白质处理对膨化玉米粉冲洗特性的影响
IF 3.9 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-19 DOI: 10.1016/j.jcs.2024.104008
Yang Ye, Xiaoqin Liu, Guixiang Jia, Yidan Zheng, Xinyue Gou, Yang Wang

To address issues such as clumping, spoon adherence, and the formation of powdery packets in puffed corn flour (PCF), this study incorporated three exogenous proteins—egg white powder (EWP), soybean protein isolate (SPI), and whey protein isolate (WPI)—and applied ultrasonication. This treatment improved PCF's physicochemical properties and microstructure. Ultrasonication increased the starch resistance and reduced the short-range ordering of the samples, as evidenced by in vitro digestion results, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR) data. Unexpectedly, the sonicated samples exhibited an A + V crystal structure with diffraction peaks near 13°,17°, and 19.9°. Furthermore, by using ultrasonication, the addition of EWP, SPI, and WPI raised the samples' water solubility index by 2.97%, 1.05%, and 9.87%, respectively; however, light transmittance decreased by 34.91%, 42.60%, and 25.33%. The agglomerate rate was reduced to zero; ultrasonic treatment also resulted in microparticles that were smaller and exhibited more holes and gaps on their surfaces. Additionally, the samples' breakdown and setback values increased, while their stability and anti-aging qualities decreased. These findings suggest that ultrasound combined with protein treatment significantly enhances the flushing properties of puffed cereal flours and supports their expanded use in the food industry.

为了解决膨化玉米粉(PCF)中的结块、勺子附着力和粉末包的形成等问题,本研究加入了三种外源蛋白质--鸡蛋白粉(EWP)、大豆分离蛋白(SPI)和乳清分离蛋白(WPI)--并采用超声波处理。这种处理方法改善了 PCF 的理化特性和微观结构。体外消化结果、X 射线衍射(XRD)和傅立叶变换红外光谱(FT-IR)数据证明,超声处理增加了淀粉抗性,降低了样品的短程有序性。出乎意料的是,超声处理后的样品呈现出 A + V 晶体结构,衍射峰靠近 13°、17° 和 19.9°。此外,通过超声处理,添加 EWP、SPI 和 WPI 后,样品的水溶性指数分别提高了 2.97%、1.05% 和 9.87%,但透光率分别降低了 34.91%、42.60% 和 25.33%。结块率降至零;超声波处理还导致微颗粒变小,表面出现更多的孔洞和间隙。此外,样品的分解值和后退值增加了,而稳定性和抗老化性却降低了。这些研究结果表明,超声波与蛋白质处理相结合可显著提高膨化谷物粉的冲洗性能,并支持其在食品工业中的广泛应用。
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引用次数: 0
Energy-size relationship and starch modification in planetary ball milling of quinoa 藜麦行星球磨中的能量-粒度关系和淀粉改性
IF 3.9 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-15 DOI: 10.1016/j.jcs.2024.104004
Yamila G. Sánchez , MaríaA. Loubes , Luciana C. González , Marcela P. Tolaba

The effects of speed (250–450 rpm) and time (10–50 min) on milling energy (E), particle size distribution (PSD), crystallinity degree (CD), damaged starch (DS), microstructure, hydration and pasting properties of flours obtained in a planetary ball mill (PBM) were investigated. As increasing milling severity quinoa flours showed polydispersed PSD (peaks at 417, 40 and 2 μm) with a shift towards smaller size and greater dispersion (Span: 3.0–5.2) as well as particles with rounded edges and polished surfaces (SEM images). The relationship E − D50 were satisfactorily predicted by the Walker's equation. The hydration tests as function of temperature and milling conditions revealed a complex flour behavior due to differences in PSD, composition, and DS content. The increasing milling energy caused a decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of up to 36%, 29%, and 25%, respectively. Significant correlations between flour attributes were found (DS-CD, r = −0.83, p < 0.01; PV-D50, r = 0.92, p < 0.01; TV-D50, r = 0.94, p < 0.01; FV-D50, r = 0.90, p < 0.01) denoted the increase of starch degradation as milling severity rises. These results can be used to improve the manufacture and the selection criteria of quinoa flour with specific functional attributes.

研究了转速(250-450 转/分钟)和时间(10-50 分钟)对行星式球磨机(PBM)中获得的面粉的研磨能量(E)、粒度分布(PSD)、结晶度(CD)、受损淀粉(DS)、微观结构、水合作用和糊化性能的影响。随着研磨程度的增加,藜麦粉显示出多分散的 PSD(峰值分别为 417、40 和 2 μm),并向更小的尺寸和更大的分散度(Span:3.0-5.2)以及具有圆形边缘和抛光表面的颗粒转变(SEM 图像)。沃克方程对 E - D50 关系的预测令人满意。由于 PSD、成分和 DS 含量的不同,水合测试与温度和研磨条件的函数关系揭示了面粉的复杂行为。研磨能量的增加导致峰值粘度 (PV)、谷值粘度 (TV) 和最终粘度 (FV) 下降,降幅分别高达 36%、29% 和 25%。面粉属性之间存在显著的相关性(DS-CD,r = -0.83,p <0.01;PV-D50,r = 0.92,p <0.01;TV-D50,r = 0.94,p <0.01;FV-D50,r = 0.90,p <0.01),表明淀粉降解会随着研磨强度的增加而增加。这些结果可用于改进具有特定功能特性的藜麦粉的生产和选择标准。
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引用次数: 0
Effect of exogenous protein substitution in glutinous rice cake: Batter rheology, structure, and retrogradation behavior 糯米糕中外源性蛋白质替代物的影响:面糊流变学、结构和逆变行为
IF 3.9 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-14 DOI: 10.1016/j.jcs.2024.104006
Yingnan Liu , Shulei Yu , Pengfei Jiang , Baoshang Fu , Libo Qi , Shan Shang

The investigation focused on the batter properties, baking performance, and retrogradation behavior of gluten-free sponge cakes after partial (15%, 30%, and 45%) substitution of glutinous rice flour with exogenous protein, including whey protein isolates (WPI), egg white protein (EWP), and soy protein isolate (SPI). The viscoelastic properties and apparent viscosity of batter positively correlated with substitution levels, with SPI superior to WPI and EWP. WPI could produce batter with smaller and more evenly distributed cells, contributing to an enhanced specific volume of the cake due to its stabilizing effect of gas cells to form viscoelastic films. The gelatinization of starch granules proved to be constrained by the incorporation of WPI and SPI, as evidenced by elevated gelatinization temperature and reduced enthalpy change values. The restriction could be attributed to the strengthened hydrogen bonding between the starch and protein, which subsequently alleviated the retrogradation and water redistribution of cake during storage. An unpleasant eggy or beany flavor of cake was generated at 45% substitutions of EWP and SPI. In comparison, 30% WPI contributed to an appealing aroma and mouthfeel, making it a nutritional supplement to enhance its protein content while maintaining its fine sensory attributes of gluten-free sponge cake.

研究的重点是部分(15%、30% 和 45%)用外源蛋白(包括乳清蛋白分离物(WPI)、蛋清蛋白(EWP)和大豆蛋白分离物(SPI))替代糯米粉后无麸质海绵蛋糕的面糊特性、烘焙性能和逆变行为。面糊的粘弹性能和表观粘度与替代水平呈正相关,其中 SPI 优于 WPI 和 EWP。WPI 能生产出细胞更小、分布更均匀的面糊,由于其稳定气孔形成粘弹性薄膜的作用,有助于提高蛋糕的比容。事实证明,加入 WPI 和 SPI 后,淀粉颗粒的糊化受到限制,具体表现为糊化温度升高,焓变值降低。这种限制可归因于淀粉和蛋白质之间氢键的加强,从而缓解了贮藏过程中蛋糕的逆降解和水分再分布。当 EWP 和 SPI 的替代率达到 45% 时,蛋糕会产生令人不愉快的蛋味或豆腥味。相比之下,30% 的 WPI 能带来诱人的香味和口感,使其成为一种营养补充剂,在提高蛋白质含量的同时保持无筋海绵蛋糕的优良感官特性。
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引用次数: 0
Ultrasound-assisted process of dough for noodle production: Textural properties, moisture distribution, microstructure and cooking quality 超声波辅助面团生产工艺:纹理特性、水分分布、微观结构和烹饪质量
IF 3.9 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-14 DOI: 10.1016/j.jcs.2024.104005
Xingguo Li , Pengfei Liu , Zhouya Bai , Denglin Luo , Chonghui Yue , Yanhui Wu , Peiyan Li , Libo Wang , Sihai Han

To address certain issues in noodle processing, a self-designed vibrating plate ultrasound equipment was implemented in dough processing. This study investigated the effects of ultrasound on the disulfide bond, secondary structures, and microstructures of noodles, as well as their qualities including texture, tensile strength, and cooking characteristics. The objective was to uncover the underlying mechanisms by which ultrasound assistance enhances dough processing. The findings indicated that ultrasonic treatment enhanced noodle quality, especially under the action of medium-intensity ultrasound treatment (MUS, 330 W; 30 s). After undergoing MUS, the noodles exhibited a notable enhancement in tensile force and stretching distance by 21.2% and 49.3% compared with the control group. MUS treatment facilitated the transformation of loosely bound water to tightly bound water during dough processing. LUS, MUS, HUS treatment led to a notable increase in β-turn and β-sheet contents. Moreover, the microstructure of noodles exhibited enhanced stability and orderliness, characterized by reduced exposed starch particles and smaller pore size. Noodles treated with MUS received the highest sensory scores. Overall, the vibrating plate ultrasonic-assisted dough processing technology demonstrated improvement in the quality of noodles, offering an innovative strategy for enhancing flour product production.

为了解决面条加工中的某些问题,在面团加工中采用了自行设计的振动板超声设备。本研究调查了超声波对面条的二硫键、次生结构和微观结构的影响,以及面条的质地、拉伸强度和烹饪特性。研究的目的是揭示超声波辅助增强面团加工的内在机制。研究结果表明,超声波处理可提高面条质量,尤其是在中等强度超声波处理(MUS,330 瓦;30 秒)的作用下。与对照组相比,经过中等强度超声波处理的面条在拉伸力和拉伸距离方面分别显著提高了 21.2% 和 49.3%。MUS处理促进了面团加工过程中松散结合水向紧密结合水的转化。LUS、MUS和HUS处理显著提高了β-匝数和β-片含量。此外,面条的微观结构表现出更高的稳定性和有序性,其特点是裸露的淀粉颗粒减少,孔径变小。用 MUS 处理的面条感官评分最高。总之,振动盘超声波辅助面团加工技术改善了面条的质量,为提高面粉产品的生产提供了一种创新策略。
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引用次数: 0
Alleles at the GLI loci are reliable genetic markers with great resolution GLI 基因座上的等位基因是可靠的遗传标记,具有很高的分辨率
IF 3.9 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-08-13 DOI: 10.1016/j.jcs.2024.104003
E. Metakovsky , L. Pascual , V.A. Melnik , L.V. Dedova , P. Vaccino , C.W. Wrigley

The use of multiple allelism at the GLI loci encoding the grain storage proteins, gliadins, makes it possible, theoretically, to distinguish between half a billion of common wheat homozygous genotypes differing among them at least at one GLI locus. No one case of the identity of gliadin genotype was observed among unrelated cultivars in our study of about 1000 registered world-wide cultivars (seed-by-seed analysis). Three well-distinguished types of intra-varietal non-uniformity were observed in the material studied: authentic biotypes (morphologically identical lines appearing as a result of segregation of heterozygous genotype produced by breeder), single foreign seeds (admixtures), and different types of mutant seeds carrying mutations at the GLI loci. Another finding made using gliadin alleles for wheat genotype identification is that wheat polymorphism is not proportionally distributed around the world. Groups of cultivars bred in different countries and even in regions of the same country might differ strongly in the frequency of specific alleles at the GLI loci. The possibility of a role of some alleles at the GLI loci in determining dough quality is discussed.

利用编码谷物贮藏蛋白--麦胶蛋白的 GLI 基因座上的多等位基因,理论上可以区分 5 亿个普通小麦同源基因型,它们之间至少在一个 GLI 基因座上存在差异。在我们对全球约 1000 个注册栽培品种的研究中(逐种子分析),没有发现一个不相关栽培品种的麦胶蛋白基因型相同。在所研究的材料中,我们观察到了三种明显的变异内不一致性:真实的生物型(由于育种者产生的杂合基因型的分离而出现的形态相同的品系)、单一的外来种子(混种)和携带 GLI 基因座突变的不同类型的突变种子。利用麦胶蛋白等位基因鉴定小麦基因型的另一个发现是,小麦的多态性在世界各地的分布并不均衡。在不同国家甚至同一国家的不同地区培育出的栽培品种组,其 GLI 基因座上特定等位基因的频率可能会有很大差异。本文讨论了 GLI 基因座上的某些等位基因在决定面团质量方面发挥作用的可能性。
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引用次数: 0
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Journal of Cereal Science
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