Micronutrient malnutrition, particularly iron (Fe) and zinc (Zn) deficiencies, remains a major nutritional concern in rice-consuming regions. This study aimed to evaluate the genotypic variation in Fe, Zn, and Phytic acid (PA) content, as well as their influence on mineral bioavailability, across 25 popular rice genotypes developed at the ICAR-Central Rice Research Institute (CRRI), Cuttack, India. This emphasizes the role of phytate-to-iron (PA: Fe) and phytate-to-zinc (PA: Zn) molar ratios on mineral bioavailability. Brown and milled rice samples were analyzed to quantify mineral loss during milling and to evaluate the relationships among Fe, Zn, and PA contents. Results revealed a significant variation in mineral and PA content across genotypes, where milling drastically reduced the Fe (83–90 %) and Zn (38–55 %) concentrations, highlighting the detrimental effect of milling on the nutritional quality of rice. A strong inverse correlation was observed between PA content and percentage Fe/Zn bioavailability (r = −0.89 and −0.92, respectively). Among such genotypes, CR Dhan 312 and CR Dhan 1014 exhibited low PA levels, favourable PA: Fe and PA: Zn molar ratios, and high mineral bioavailability. Therefore, the study demonstrates that genotypic variation strongly influences the mineral-phytate balance and hence bioavailability in rice. Identification of low-PA, high mineral genotypes with optimal PA: Fe and PA: Zn ratios could support the development of biofortified rice varieties with improved mineral bioavailability to alleviate micronutrient malnutrition in rice-dependent population.
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