Pub Date : 2024-10-05DOI: 10.1016/j.jcs.2024.104036
Chong Deng , Shunjing Luo , Xiuting Hu , Chengmei Liu
This work investigated the effect of treatment by sodium carbonate on grinding of wheat bran and the related mechanism. Specifically, wheat bran was treated by sodium carbonate solutions at the ratio of 1:2 to make addition of sodium carbonate at 0.0%, 4.0%, 8.0%, 12.0%, 15.0%, and 30.0% based on the mass of bran, dried, and ground using the universal high-speed grinder. It was found that treatment by sodium carbonate significantly reduced the particle size of ground bran and this effect was positively correlated with the sodium carbonate content, which suggested that treatment by sodium carbonate promoted the grinding of wheat bran. Besides, treatment by sodium carbonate improved the hardness of bran. On the other hand, treatment by sodium carbonate damaged the crystal structure, the cell wall and lignin of bran. Moreover, treatment by sodium carbonate converted bound phenolics, including ferulic acid, into free phenolics and insoluble dietary fiber, including unextractable arabinoxylan, into soluble dietary fiber. These results suggested that treatment by sodium carbonate had already damaged the structure of bran and altered the texture of bran before the mechanical stress during grinding was applied. As a result, treatment by sodium carbonate promoted the grinding of wheat bran.
{"title":"Promoting the grinding of wheat bran through treatment by sodium carbonate and its mechanism","authors":"Chong Deng , Shunjing Luo , Xiuting Hu , Chengmei Liu","doi":"10.1016/j.jcs.2024.104036","DOIUrl":"10.1016/j.jcs.2024.104036","url":null,"abstract":"<div><div>This work investigated the effect of treatment by sodium carbonate on grinding of wheat bran and the related mechanism. Specifically, wheat bran was treated by sodium carbonate solutions at the ratio of 1:2 to make addition of sodium carbonate at 0.0%, 4.0%, 8.0%, 12.0%, 15.0%, and 30.0% based on the mass of bran, dried, and ground using the universal high-speed grinder. It was found that treatment by sodium carbonate significantly reduced the particle size of ground bran and this effect was positively correlated with the sodium carbonate content, which suggested that treatment by sodium carbonate promoted the grinding of wheat bran. Besides, treatment by sodium carbonate improved the hardness of bran. On the other hand, treatment by sodium carbonate damaged the crystal structure, the cell wall and lignin of bran. Moreover, treatment by sodium carbonate converted bound phenolics, including ferulic acid, into free phenolics and insoluble dietary fiber, including unextractable arabinoxylan, into soluble dietary fiber. These results suggested that treatment by sodium carbonate had already damaged the structure of bran and altered the texture of bran before the mechanical stress during grinding was applied. As a result, treatment by sodium carbonate promoted the grinding of wheat bran.</div></div>","PeriodicalId":15285,"journal":{"name":"Journal of Cereal Science","volume":"120 ","pages":"Article 104036"},"PeriodicalIF":3.9,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-05DOI: 10.1016/j.jcs.2024.104033
Nikitha Modupalli, Ruslan Mehadi Galib, Rahul Sen, Scott Lafontaine, Md Mahfuzur Rahman
Rice bran is a milling byproduct and rice bran protein (RBP) is a promising source of plant-based protein. This study investigated the impact of alkaline and acid extractions followed by isoelectric point (IEP) and heat coagulation precipitations on the quality and functionalities of RBP. Alkaline extraction, followed by IEP and heat coagulation, showed the highest protein recovery of 48.77%, which is double that of conventional alkaline extraction and IEP. The FTIR data showed that β-sheet and α-helix content reduced; however, random coil and β-turn increased by acid and heat addition. Addition of heat coagulation to IEP after alkaline extraction increased solubility from 62.94% to 94.74%. However, the emulsification and surface hydrophobicity were decreased during heat and acid-assisted extraction. The in-vitro digestibility was the highest at 83.57% in alkaline-acid extraction, followed by heat and IEP. The aroma profile of RBP shows a complex mixture of ethanol-2-butoxy, 2-methyl-4-vinyl-phenol, 2,4—heptagonal, (E,E)-, Undecane, and 5-methyl to form a typical flavor with waxy and rancid undernotes. The total amount of volatile compounds in conventional extraction was 1.97 μg/g, while alkaline-acid extraction with IEP had only 0.87 μg/g. The RBP from alkaline-acid extraction with IEP showed the least volatile compounds and most neutral proteins.
{"title":"Improving yield, functional properties, and aroma profile of rice bran protein through innovative extraction and precipitation methods","authors":"Nikitha Modupalli, Ruslan Mehadi Galib, Rahul Sen, Scott Lafontaine, Md Mahfuzur Rahman","doi":"10.1016/j.jcs.2024.104033","DOIUrl":"10.1016/j.jcs.2024.104033","url":null,"abstract":"<div><div>Rice bran is a milling byproduct and rice bran protein (RBP) is a promising source of plant-based protein. This study investigated the impact of alkaline and acid extractions followed by isoelectric point (IEP) and heat coagulation precipitations on the quality and functionalities of RBP. Alkaline extraction, followed by IEP and heat coagulation, showed the highest protein recovery of 48.77%, which is double that of conventional alkaline extraction and IEP. The FTIR data showed that β-sheet and α-helix content reduced; however, random coil and β-turn increased by acid and heat addition. Addition of heat coagulation to IEP after alkaline extraction increased solubility from 62.94% to 94.74%. However, the emulsification and surface hydrophobicity were decreased during heat and acid-assisted extraction. The in-vitro digestibility was the highest at 83.57% in alkaline-acid extraction, followed by heat and IEP. The aroma profile of RBP shows a complex mixture of ethanol-2-butoxy, 2-methyl-4-vinyl-phenol, 2,4—heptagonal, (E,E)-, Undecane, and 5-methyl to form a typical flavor with waxy and rancid undernotes. The total amount of volatile compounds in conventional extraction was 1.97 μg/g, while alkaline-acid extraction with IEP had only 0.87 μg/g. The RBP from alkaline-acid extraction with IEP showed the least volatile compounds and most neutral proteins.</div></div>","PeriodicalId":15285,"journal":{"name":"Journal of Cereal Science","volume":"120 ","pages":"Article 104033"},"PeriodicalIF":3.9,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-04DOI: 10.1016/j.jcs.2024.104034
R. Kaasik, M. Tikhonova, R. Koppel, A. Ingver
In Estonian local mills, protein content (PC) and wet gluten content (WGC) are among the main indicators considered and evaluated when purchasing grains. Protein composition also significantly affects baking quality. The baking quality of ten winter wheat (WW) and spring wheat (SW) varieties was studied at the Center of Estonian Rural Research and Knowledge (METK). PC, WGC, gluten index (GI), and dough stability time (DST) were higher in SW varieties. PC was positively correlated with WGC and loaf volume (LV) in both wheat types. PC and WGC were negatively correlated with GI and positively correlated with DST only in WW. The viscoelastic properties of dough are linked to its high- and low molecular weight glutenin subunits (HMW-GS and LMW-GS). The allelic composition of the glutenin genes was determined using DNA markers. All the studied varieties had the allele Glu-D1d and additional allele Glu-D1a was found in the variety ‘Voore’. Fourteen varieties had Glu-A1a and b alleles associated with improved backing quality. Alleles Glu-B1с and Glu-B1b were found in WW varieties. SW varieties carried alleles Glu-B1al, b, с and h. The average QS was higher in SW. Eight varieties had null alleles Glu-A1c, Glu-A3e, and Glu-B3j, which negatively affected bread-making quality.
{"title":"The association of glutenin subunit composition to baking quality traits of spring and winter wheat in Estonia","authors":"R. Kaasik, M. Tikhonova, R. Koppel, A. Ingver","doi":"10.1016/j.jcs.2024.104034","DOIUrl":"10.1016/j.jcs.2024.104034","url":null,"abstract":"<div><div>In Estonian local mills, protein content (PC) and wet gluten content (WGC) are among the main indicators considered and evaluated when purchasing grains. Protein composition also significantly affects baking quality. The baking quality of ten winter wheat (WW) and spring wheat (SW) varieties was studied at the Center of Estonian Rural Research and Knowledge (METK). PC, WGC, gluten index (GI), and dough stability time (DST) were higher in SW varieties. PC was positively correlated with WGC and loaf volume (LV) in both wheat types. PC and WGC were negatively correlated with GI and positively correlated with DST only in WW. The viscoelastic properties of dough are linked to its high- and low molecular weight glutenin subunits (HMW-GS and LMW-GS). The allelic composition of the glutenin genes was determined using DNA markers. All the studied varieties had the allele <em>Glu-D1d</em> and additional allele <em>Glu-D1a</em> was found in the variety ‘Voore’. Fourteen varieties had <em>Glu-A1a</em> and <em>b</em> alleles associated with improved backing quality. Alleles <em>Glu-B1с</em> and <em>Glu-B1b</em> were found in WW varieties. SW varieties carried alleles <em>Glu-B1al</em>, <em>b</em>, <em>с</em> and h. The average QS was higher in SW. Eight varieties had null alleles <em>Glu-A1c, Glu-A3e,</em> and <em>Glu-B3j</em>, which negatively affected bread-making quality.</div></div>","PeriodicalId":15285,"journal":{"name":"Journal of Cereal Science","volume":"120 ","pages":"Article 104034"},"PeriodicalIF":3.9,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arbuscular mycorrhizal fungi (AMF) inoculation has important regulatory influence on plant growth and nitrogen uptake, which are intimately associated with soft wheat yield and quality. The study aimed to investigate the impact of AMF inoculation on soft wheat grain yield, protein compositions, protein quality, secondary structure of gluten, dough properties and biscuits baking quality. In this study, AMF inoculation significantly increased grain yield. AMF inoculation decreased grain protein content by 5.1%, glutenin content by 8.5%, GMP by 14.3%, and HMW-GS by 8.8% compared with CK. Conversely, AMF inoculation increased the ratio of gliadin/glutenin. Further analysis showed AMF treatment had a looser gluten network than CK, significantly reduced β-sheet and β-turn content, while α-helix content increased. The nutritional index of grain protein was slightly reduced under AMF inoculation, yet the biological value was improved. Reduced wet gluten content, gluten performance index and dough development time under AMF treatment through regulation of soft wheat protein composition and gluten protein structure. Therefore, biscuits in AMF treatment showed reduced thickness, hardness, significantly greater spread ratio and sensory score compared with CK. These indicate that AMF application would be a beneficial agronomic practice to synergistically optimize yield and grain quality in soft wheat.
接种丛枝菌根真菌(AMF)对植物生长和氮吸收具有重要的调节作用,而植物生长和氮吸收与软质小麦的产量和品质密切相关。本研究旨在探讨接种 AMF 对软质小麦籽粒产量、蛋白质组成、蛋白质质量、面筋次生结构、面团特性和饼干烘焙质量的影响。在这项研究中,接种 AMF 能显著提高谷物产量。与 CK 相比,接种 AMF 可使谷物蛋白质含量降低 5.1%,谷蛋白含量降低 8.5%,GMP 降低 14.3%,HMW-GS 降低 8.8%。相反,接种 AMF 会增加谷胶蛋白/谷蛋白的比例。进一步分析表明,与 CK 相比,AMF 处理的谷蛋白网络更松散,β-片和 β-匝含量显著减少,而 α-螺旋含量增加。接种 AMF 后,谷物蛋白质的营养指数略有降低,但生物价值有所提高。通过调节软质小麦蛋白质组成和面筋蛋白质结构,AMF 处理下的湿面筋含量、面筋性能指标和面团发育时间均有所降低。因此,与 CK 相比,AMF 处理的饼干厚度、硬度降低,铺展率和感官评分显著提高。这表明,施用 AMF 是一种有益的农艺措施,可协同优化软质小麦的产量和谷物品质。
{"title":"Arbuscular mycorrhizal fungi affected soft wheat quality properties and nutritional index by regulating the composition and secondary structure of protein","authors":"Jiawen Xue, Lanxin Mei, Xinru Wang, Yingxin Zhong, Mei Huang, Xiao Wang, Jian Cai, Tingbo Dai, Qin Zhou, Dong Jiang","doi":"10.1016/j.jcs.2024.104032","DOIUrl":"10.1016/j.jcs.2024.104032","url":null,"abstract":"<div><div>Arbuscular mycorrhizal fungi (AMF) inoculation has important regulatory influence on plant growth and nitrogen uptake, which are intimately associated with soft wheat yield and quality. The study aimed to investigate the impact of AMF inoculation on soft wheat grain yield, protein compositions, protein quality, secondary structure of gluten, dough properties and biscuits baking quality. In this study, AMF inoculation significantly increased grain yield. AMF inoculation decreased grain protein content by 5.1%, glutenin content by 8.5%, GMP by 14.3%, and HMW-GS by 8.8% compared with CK. Conversely, AMF inoculation increased the ratio of gliadin/glutenin. Further analysis showed AMF treatment had a looser gluten network than CK, significantly reduced β-sheet and β-turn content, while α-helix content increased. The nutritional index of grain protein was slightly reduced under AMF inoculation, yet the biological value was improved. Reduced wet gluten content, gluten performance index and dough development time under AMF treatment through regulation of soft wheat protein composition and gluten protein structure. Therefore, biscuits in AMF treatment showed reduced thickness, hardness, significantly greater spread ratio and sensory score compared with CK. These indicate that AMF application would be a beneficial agronomic practice to synergistically optimize yield and grain quality in soft wheat.</div></div>","PeriodicalId":15285,"journal":{"name":"Journal of Cereal Science","volume":"120 ","pages":"Article 104032"},"PeriodicalIF":3.9,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, the impact of varying particle size fractions—namely fine, medium, and coarse fractions—of banana peel powder (BPP) on the physicochemical properties and sensory profiles of transglutaminase-induced protein-crosslinked instant noodles was investigated. Gravimetric measurements revealed that BPP incorporation led to higher ash and total dietary fiber content. Tensile force evaluations of cooked noodles demonstrated that fine BPP crosslinked noodles exhibited superior tensile strength, while crosslinked noodles with coarse BPP maintained similar tensile force to non-crosslinked wheat noodles. Scanning electron microscopy revealed a denser surface within the noodle samples. Enzymatic crosslinking increased β-sheet protein secondary structures, while BPP incorporation reduced the short-range ordered starch structure. BPP also influenced color properties, with greater lightness reduction observed in medium and coarse BPP fractions. Sensory evaluations indicated that consumers favored noodles containing fine BPP particles over the medium and coarse BPP fractions, as revealed by hedonic sensory evaluation. Additionally, penalty analysis of sensory data obtained from the check-all-that-apply method highlighted favorable and unfavorable sensory attributes of the noodles. Overall, this study provides valuable insights for the development of healthier instant noodle products.
{"title":"Physicochemical evaluation and sensory profiling of protein-crosslinked instant noodles enriched with particle size-fractionated banana peel powder","authors":"Kurnia Ramadhan, Nasma Sadah, Nurul Asiah, Ardiansyah","doi":"10.1016/j.jcs.2024.104029","DOIUrl":"10.1016/j.jcs.2024.104029","url":null,"abstract":"<div><div>In this study, the impact of varying particle size fractions—namely fine, medium, and coarse fractions—of banana peel powder (BPP) on the physicochemical properties and sensory profiles of transglutaminase-induced protein-crosslinked instant noodles was investigated. Gravimetric measurements revealed that BPP incorporation led to higher ash and total dietary fiber content. Tensile force evaluations of cooked noodles demonstrated that fine BPP crosslinked noodles exhibited superior tensile strength, while crosslinked noodles with coarse BPP maintained similar tensile force to non-crosslinked wheat noodles. Scanning electron microscopy revealed a denser surface within the noodle samples. Enzymatic crosslinking increased β-sheet protein secondary structures, while BPP incorporation reduced the short-range ordered starch structure. BPP also influenced color properties, with greater lightness reduction observed in medium and coarse BPP fractions. Sensory evaluations indicated that consumers favored noodles containing fine BPP particles over the medium and coarse BPP fractions, as revealed by hedonic sensory evaluation. Additionally, penalty analysis of sensory data obtained from the check-all-that-apply method highlighted favorable and unfavorable sensory attributes of the noodles. Overall, this study provides valuable insights for the development of healthier instant noodle products.</div></div>","PeriodicalId":15285,"journal":{"name":"Journal of Cereal Science","volume":"120 ","pages":"Article 104029"},"PeriodicalIF":3.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tef, a nutritious gluten-free cereal essential for making Ethiopia's traditional injera, varies in quality due to varieties and growing conditions. This study examined 35 tef varieties, revealing how variety, location, and season affect grain attributes and flour quality. Insights from growing tef in diverse Ethiopian agro-ecologies enhance understanding of its adaptability and quality. Physical and functional properties, including grain size, density, and flour absorption capacities, were analyzed, offering a comprehensive view of tef's potential in varying environments. Variety significantly affects tef characteristics, with genotype playing a major role in trait variation, influencing grain size, weight, and color. Sieve analysis showed that up to 45.78% of grain mass remained on larger sieves (425–600μm). Color metrics for tef varied, with white tef being lighter and redder than brown. Environmental factors like location and season influenced sieve separation outcomes and grain characteristics, except for the smallest sieve size and color parameters. The study also noted a relationship between grain size and color, with smaller, brown grains having lower lightness and yellowness. This study provides essential data: for future improvement and management of the tef; for quality assurance for consumers, processors, regulators and traders; and for designing of effective cleaning and processing techniques.
{"title":"Grain and flour physical and functional characteristics of Tef [Eragrostis tef (Zucc) Trotter] varieties of Ethiopia","authors":"Tamrat Kore Menjaye , Kebebew Assefa , Oli Legese , Muhaba Seifu , Worku Kebede , Workineh Abebe , Lamsgen Yegrem , Deribe Mengistu , Semhal Marsha , Teshome Mesfin , Solomon Chanyalew","doi":"10.1016/j.jcs.2024.104030","DOIUrl":"10.1016/j.jcs.2024.104030","url":null,"abstract":"<div><div>Tef, a nutritious gluten-free cereal essential for making Ethiopia's traditional <em>injera,</em> varies in quality due to varieties and growing conditions. This study examined 35 tef varieties, revealing how variety, location, and season affect grain attributes and flour quality. Insights from growing tef in diverse Ethiopian agro-ecologies enhance understanding of its adaptability and quality. Physical and functional properties, including grain size, density, and flour absorption capacities, were analyzed, offering a comprehensive view of tef's potential in varying environments. Variety significantly affects tef characteristics, with genotype playing a major role in trait variation, influencing grain size, weight, and color. Sieve analysis showed that up to 45.78% of grain mass remained on larger sieves (425–600μm). Color metrics for tef varied, with white tef being lighter and redder than brown. Environmental factors like location and season influenced sieve separation outcomes and grain characteristics, except for the smallest sieve size and color parameters. The study also noted a relationship between grain size and color, with smaller, brown grains having lower lightness and yellowness. This study provides essential data: for future improvement and management of the tef; for quality assurance for consumers, processors, regulators and traders; and for designing of effective cleaning and processing techniques.</div></div>","PeriodicalId":15285,"journal":{"name":"Journal of Cereal Science","volume":"120 ","pages":"Article 104030"},"PeriodicalIF":3.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-18DOI: 10.1016/j.jcs.2024.104028
Nadia Calabriso , Marika Massaro , Egeria Scoditti , Stefano Quarta , Barbara Laddomada , Aurelia Scarano , Samuela Palombieri , Francesco Sestili , Maria Annunziata Carluccio
Newly developed high amylose durum wheat genotypes, characterized by a high content of resistant starch, is gaining interest as a healthy ingredient for functional food. The objective of the present study was to investigate the effects of Svevo high amylose phenolic extracts (HAPE) on endothelial activation, the first obligatory step of the atherosclerotic process, investigating the underlying mechanisms of action. To this aim, human microvascular endothelial cells were treated with HAPE (1–10 μg/mL) and then challenged with the pro-inflammatory and pro-atherogenic cytokine TNF-α. Endothelium-leukocyte adhesion, the expression of endothelial inflammatory mediators, intracellular reactive oxygen species (ROS) levels and the activation of nuclear factor (NF)-κB were evaluated by multiple assays. The results showed that, HAPE, already at 5 μg/mL, suppressed the TNF-α stimulated expression of endothelial adhesion molecules as well as the adhesion of leukocytes to endothelial cells by more than 50% in comparison with TNF-α alone. These effects were associated with a significant (p < 0.05) reduction of intracellular ROS levels and NF-κB activation. In summary, our findings underscore the potential of Svevo high amylose as a functional food able to blunt endothelial activation, by inhibiting the concerted expression of endothelial markers involved in leukocyte recruitment and early atherogenesis.
{"title":"Exploring the health benefits of high amylose wheat phenolic extract in human endothelial cell model: Inhibitory effects on endothelial activation","authors":"Nadia Calabriso , Marika Massaro , Egeria Scoditti , Stefano Quarta , Barbara Laddomada , Aurelia Scarano , Samuela Palombieri , Francesco Sestili , Maria Annunziata Carluccio","doi":"10.1016/j.jcs.2024.104028","DOIUrl":"10.1016/j.jcs.2024.104028","url":null,"abstract":"<div><div>Newly developed high amylose durum wheat genotypes, characterized by a high content of resistant starch, is gaining interest as a healthy ingredient for functional food. The objective of the present study was to investigate the effects of Svevo high amylose phenolic extracts (HAPE) on endothelial activation, the first obligatory step of the atherosclerotic process, investigating the underlying mechanisms of action. To this aim, human microvascular endothelial cells were treated with HAPE (1–10 μg/mL) and then challenged with the pro-inflammatory and pro-atherogenic cytokine TNF-α. Endothelium-leukocyte adhesion, the expression of endothelial inflammatory mediators, intracellular reactive oxygen species (ROS) levels and the activation of nuclear factor (NF)-κB were evaluated by multiple assays. The results showed that, HAPE, already at 5 μg/mL, suppressed the TNF-α stimulated expression of endothelial adhesion molecules as well as the adhesion of leukocytes to endothelial cells by more than 50% in comparison with TNF-α alone. These effects were associated with a significant (p < 0.05) reduction of intracellular ROS levels and NF-κB activation. In summary, our findings underscore the potential of Svevo high amylose as a functional food able to blunt endothelial activation, by inhibiting the concerted expression of endothelial markers involved in leukocyte recruitment and early atherogenesis.</div></div>","PeriodicalId":15285,"journal":{"name":"Journal of Cereal Science","volume":"120 ","pages":"Article 104028"},"PeriodicalIF":3.9,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-17DOI: 10.1016/j.jcs.2024.104027
Jiawei Tang , Shixin Song , Jingwei Zhang , Ye Li , Li Tao , Qi Wu , Yilin Zhao , Lei Yu
Buckwheat protein (BP) was studied in relation to oil phase volume fractions for the preparation of Antarctic krill oil (AKO) emulsions. The physicochemical properties and microstructure of BP-AKO emulsions were examined using zeta potential, droplet size, rheology, and laser confocal microscopy. The bioavailability of astaxanthin in AKO was assessed through in vitro digestion experiments. Among the emulsions tested, the BP-AKO (5–60%) emulsion, containing 5% protein and 60% oil phase, displayed the smallest particle size (1.86 μm), highest absolute zeta potential value (47.88 mV), and greatest interfacial protein adsorption rate (89.00%), indicating superior stability. Oxidative stability experiments revealed that the BP-AKO (5–60%) emulsion exhibited the lowest production of lipid hydroperoxides and malondialdehyde, reflecting excellent oxidative stability. Furthermore, the highest release of free fatty acids from the BP-AKO (5–60%) emulsion occurred during in vitro digestion, peaking at 70.17% after 120 min. The bioavailability of astaxanthin also reached a maximum value of 50.37%. This study provides experimental support for the use of macromolecular cereal proteins as emulsifiers to stabilize Antarctic krill oil.
在制备南极磷虾油(AKO)乳剂时,研究了荞麦蛋白(BP)与油相体积分数的关系。使用zeta电位、液滴大小、流变学和激光共聚焦显微镜检测了BP-AKO乳剂的理化性质和微观结构。通过体外消化实验评估了虾青素在 AKO 中的生物利用率。在测试的乳液中,含有5%蛋白质和60%油相的BP-AKO(5-60%)乳液显示出最小的粒径(1.86 μm)、最高的绝对ZETA电位值(47.88 mV)和最大的界面蛋白质吸附率(89.00%),表明其具有卓越的稳定性。氧化稳定性实验表明,BP-AKO(5-60%)乳液的脂质氢过氧化物和丙二醛生成量最低,反映了其出色的氧化稳定性。此外,在体外消化过程中,BP-AKO(5-60%)乳液中游离脂肪酸的释放量最高,120 分钟后达到 70.17%。虾青素的生物利用率也达到了 50.37% 的最高值。这项研究为使用大分子谷物蛋白作为乳化剂来稳定南极磷虾油提供了实验支持。
{"title":"Buckwheat protein as macromolecular emulsifier to stabilize antarctic krill oil-loaded emulsion: Study on physicochemical properties, microstructure, and functional properties","authors":"Jiawei Tang , Shixin Song , Jingwei Zhang , Ye Li , Li Tao , Qi Wu , Yilin Zhao , Lei Yu","doi":"10.1016/j.jcs.2024.104027","DOIUrl":"10.1016/j.jcs.2024.104027","url":null,"abstract":"<div><div>Buckwheat protein (BP) was studied in relation to oil phase volume fractions for the preparation of Antarctic krill oil (AKO) emulsions. The physicochemical properties and microstructure of BP-AKO emulsions were examined using zeta potential, droplet size, rheology, and laser confocal microscopy. The bioavailability of astaxanthin in AKO was assessed through in vitro digestion experiments. Among the emulsions tested, the BP-AKO (5–60%) emulsion, containing 5% protein and 60% oil phase, displayed the smallest particle size (1.86 μm), highest absolute zeta potential value (47.88 mV), and greatest interfacial protein adsorption rate (89.00%), indicating superior stability. Oxidative stability experiments revealed that the BP-AKO (5–60%) emulsion exhibited the lowest production of lipid hydroperoxides and malondialdehyde, reflecting excellent oxidative stability. Furthermore, the highest release of free fatty acids from the BP-AKO (5–60%) emulsion occurred during in vitro digestion, peaking at 70.17% after 120 min. The bioavailability of astaxanthin also reached a maximum value of 50.37%. This study provides experimental support for the use of macromolecular cereal proteins as emulsifiers to stabilize Antarctic krill oil.</div></div>","PeriodicalId":15285,"journal":{"name":"Journal of Cereal Science","volume":"120 ","pages":"Article 104027"},"PeriodicalIF":3.9,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-17DOI: 10.1016/j.jcs.2024.104023
Annegret Jannasch , Ya-Jane Wang , Sun-Ok Lee , Rohana Liyanage , Anna M. McClung
Pigmented and non-pigmented rice cultivars contain different polyphenol classes. Covalent bonding of polyphenols with glutelin, the major protein fraction in rice, can occur in pigmented rice. The effects of polyphenol class on glutelin molecular weight and size were studied via SDS-PAGE and HPSEC, respectively. The proteomic analysis of glutelin was performed via LC-MS/MS. Rice cultivars with brown (Neches), purple (HB-1) and red (Scarlett) bran colors mainly comprising phenolic acids, anthocyanins, and flavan-3-ols/proanthocyanidins, respectively, were used. Glutelin in milled rice exhibited similar molecular weight and size and proteomic identification due to the lower concentration of polyphenols. Covalent modification of glutelin by polyphenols decreased the amount of identified glutelin. Phenolic acids caused little or no structural changes of glutelin. Anthocyanins and flavan-3-ols/proanthocyanidins in whole grain rice and bran of HB-1 and Scarlett caused a more compact glutelin structure through intra-molecular bonds. Polyphenol-protein interactions may be utilized to tailor food ingredients from pigmented rice with desired rheological properties.
{"title":"Polyphenol-mediated covalent bonds on glutelin structural changes in rice with different bran colors","authors":"Annegret Jannasch , Ya-Jane Wang , Sun-Ok Lee , Rohana Liyanage , Anna M. McClung","doi":"10.1016/j.jcs.2024.104023","DOIUrl":"10.1016/j.jcs.2024.104023","url":null,"abstract":"<div><div>Pigmented and non-pigmented rice cultivars contain different polyphenol classes. Covalent bonding of polyphenols with glutelin, the major protein fraction in rice, can occur in pigmented rice. The effects of polyphenol class on glutelin molecular weight and size were studied via SDS-PAGE and HPSEC, respectively. The proteomic analysis of glutelin was performed via LC-MS/MS. Rice cultivars with brown (Neches), purple (HB-1) and red (Scarlett) bran colors mainly comprising phenolic acids, anthocyanins, and flavan-3-ols/proanthocyanidins, respectively, were used. Glutelin in milled rice exhibited similar molecular weight and size and proteomic identification due to the lower concentration of polyphenols. Covalent modification of glutelin by polyphenols decreased the amount of identified glutelin. Phenolic acids caused little or no structural changes of glutelin. Anthocyanins and flavan-3-ols/proanthocyanidins in whole grain rice and bran of HB-1 and Scarlett caused a more compact glutelin structure through intra-molecular bonds. Polyphenol-protein interactions may be utilized to tailor food ingredients from pigmented rice with desired rheological properties.</div></div>","PeriodicalId":15285,"journal":{"name":"Journal of Cereal Science","volume":"120 ","pages":"Article 104023"},"PeriodicalIF":3.9,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-16DOI: 10.1016/j.jcs.2024.104026
Yukinori Sato
{"title":"Image illumination to enhance the visual palatability and visual firmness of white bread crumbs","authors":"Yukinori Sato","doi":"10.1016/j.jcs.2024.104026","DOIUrl":"10.1016/j.jcs.2024.104026","url":null,"abstract":"","PeriodicalId":15285,"journal":{"name":"Journal of Cereal Science","volume":"120 ","pages":"Article 104026"},"PeriodicalIF":3.9,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}