Sweet corn (Zea mays L.) is a popular crop valued for its taste and versatile uses but is nutritionally limited, particularly in compounds such as beta-carotene and anthocyanins. This study aimed to develop sweet corn lines enriched with both beta-carotene and anthocyanins using marker-assisted backcross breeding. Chujak Red (rich in anthocyanins), a landrace from the North-Eastern Himalayan Region (NEHR), was crossed with a biofortified sweet corn line, DBT 17 (containing the crtRB1 allele and sh2 gene). The F2 and successive backcross generations (BC1F1 to BC2F4) were selected for the crtRB1 allele, kernel colour, and shrunken endosperm using molecular markers and phenotypic screening. Foreground selection employed allele-specific markers for crtRB1, while background selection used 92 polymorphic SSR markers across generations. A total of six stable BC2F4 lines exhibiting moderate red kernel colour, high beta-carotene, and anthocyanin contents were developed. Agronomic evaluation showed that these lines performed comparably to the recurrent parent DBT 17 for morphological traits. Biochemical analyses of these lines confirmed comparable total soluble solids (14.7–16.0 °Brix) and total sugar content (16.1–16.8 %). Furthermore, the beta-carotene content of these lines ranged from 8.30 to 10.23 μg/g, significantly higher than that of Chujak Red and comparable to DBT 17. Cyanidin-3-glucoside levels at 30 days after pollination reached up to 8.1 mg/100 g FW, close to the donor Chujak Red, thereby confirming successful pigment integration. Taken together, our study developed promising sweet corn lines rich in both anthocyanin and beta-carotene, and these lines are potential sources for functional food development and advancement of maize nutritional breeding programs.
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