This research thoroughly examined the impact of incorporating germinated oat flour on the characteristics of wheat dough and steamed buns. The results revealed that endogenous enzymes, specifically α-amylase and proteases, progressively degraded starch granules and disrupted the gluten network, resulting in a marked weakening of dough rheological properties, as evidenced by reductions in storage modulus (G′) and loss modulus (G″), decreased viscosity, and the formation of a softer, more viscous matrix. Structural analyses further confirmed a decline in starch crystallinity alongside alterations in protein secondary structure. In the steamed buns, the addition of germinated oat flour led to an increase in resistant starch content and antioxidant activity, which contributed to a lowered estimated glycemic index; however, this was accompanied by a reduction in specific volume and the occurrence of browning. A non-linear, dose-dependent effect was identified, with a critical incorporation threshold between 4 % and 6 %. The study concludes that germinated oat flour represents a promising functional ingredient for enhancing nutritional value, although its inclusion level must be carefully optimized to maintain a balance between health benefits and product quality.
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