{"title":"HepG2 cells with recombinant cytochrome P450 enzyme overexpression: Their use and limitation as in vitro liver model","authors":"Susanne Steinbrecht, S. Kammerer, J. Küpper","doi":"10.3233/JCB-189013","DOIUrl":"https://doi.org/10.3233/JCB-189013","url":null,"abstract":"","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-189013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70024601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Krüger-Genge, R. Sternitzky, G. Pindur, M. Rampling, R. Franke, F. Jung
{"title":"Erythrocyte aggregation in relation to plasma proteins and lipids","authors":"A. Krüger-Genge, R. Sternitzky, G. Pindur, M. Rampling, R. Franke, F. Jung","doi":"10.3233/JCB-189014","DOIUrl":"https://doi.org/10.3233/JCB-189014","url":null,"abstract":"","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-189014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70025172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Christin Neuber, M. Laube, C. Mamat, B. Belter, Jens Pietzsch
{"title":"Regulation of A375 melanoma cell adhesion and migration by EphB4 and EphrinB2 – insights from co-culture experiments","authors":"Christin Neuber, M. Laube, C. Mamat, B. Belter, Jens Pietzsch","doi":"10.3233/JCB-189011","DOIUrl":"https://doi.org/10.3233/JCB-189011","url":null,"abstract":"","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-189011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70024477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. V. Muravyov, I. Tikhomirova, P. Avdonin, S. V. Bulaeva, Yulia Malysheva, N. Kislov
{"title":"Cellular models of erythrocytes for studying the effect of gasotransmitters on their microrheology","authors":"A. V. Muravyov, I. Tikhomirova, P. Avdonin, S. V. Bulaeva, Yulia Malysheva, N. Kislov","doi":"10.3233/JCB-189009","DOIUrl":"https://doi.org/10.3233/JCB-189009","url":null,"abstract":"","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-189009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70024692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Spirulina platensis, a multicelluar, photosynthetic prokaryote (algae) contains a high amount of proteins, vitamins and minerals superior to many foods as e.g. soybeans. Thus, Spirulina platensis was recognized as nutritious food by the United Nations World Food Conference. Due to the high amount of nutritive ingredients Spirulina has a long history as dietary supplement. In addition, spirulina platensis is also efficiently used as forage with known effects on flesh, egg and plumage color, milk yield and fertility. The versatile utilization of the alga can be explained on the one hand with the nutrient levels and on the other hand with recognized effects as anti-viral, anti-bacterial, anti-oxidant, anti-diabetic, anti-cancer and anti-inflammatory substance. Therefore, this alga is named as “superfood”. Beyond, these algae convert carbon dioxide into organic substances and produce oxygen during their growth in alkaline and saline water thereby not wasting fresh water allowing the production in barren areas. Despite this diverse use of Spirulina platensis due to its beneficial properties, many basic mechanisms on a molecular and cellular level are not well understood and should be explored in future studies.
{"title":"Spirulina platensis, a super food?","authors":"F. Jung, A. Krüger-Genge, P. Waldeck, J. Küpper","doi":"10.3233/JCB-189012","DOIUrl":"https://doi.org/10.3233/JCB-189012","url":null,"abstract":"Spirulina platensis, a multicelluar, photosynthetic prokaryote (algae) contains a high amount of proteins, vitamins and minerals superior to many foods as e.g. soybeans. Thus, Spirulina platensis was recognized as nutritious food by the United Nations World Food Conference. Due to the high amount of nutritive ingredients Spirulina has a long history as dietary supplement. In addition, spirulina platensis is also efficiently used as forage with known effects on flesh, egg and plumage color, milk yield and fertility. The versatile utilization of the alga can be explained on the one hand with the nutrient levels and on the other hand with recognized effects as anti-viral, anti-bacterial, anti-oxidant, anti-diabetic, anti-cancer and anti-inflammatory substance. Therefore, this alga is named as “superfood”. Beyond, these algae convert carbon dioxide into organic substances and produce oxygen during their growth in alkaline and saline water thereby not wasting fresh water allowing the production in barren areas. Despite this diverse use of Spirulina platensis due to its beneficial properties, many basic mechanisms on a molecular and cellular level are not well understood and should be explored in future studies.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-189012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70024582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Ionova, V. B. Lantsova, E. Tkach, R. Ziganshin, V. Shender, E. Weaver, V. Paskova, N. Antonova, I. Strokov
{"title":"Protein spectrum of blood plasma in patients with diabetes mellitus type II","authors":"V. Ionova, V. B. Lantsova, E. Tkach, R. Ziganshin, V. Shender, E. Weaver, V. Paskova, N. Antonova, I. Strokov","doi":"10.3233/JCB-179009","DOIUrl":"https://doi.org/10.3233/JCB-179009","url":null,"abstract":"","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-179009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43748843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Krüger-Genge, F. Jung, J. Küpper, Christian Lehmann, R. Franke
{"title":"Actin type and distribution in erythrocytes","authors":"A. Krüger-Genge, F. Jung, J. Küpper, Christian Lehmann, R. Franke","doi":"10.3233/JCB-179014","DOIUrl":"https://doi.org/10.3233/JCB-179014","url":null,"abstract":"","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-179014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48725706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Müller, Miriam Hübner, S. Kuderer, Marie Prechtl, J. Rox, J. Fischer, F. Wenzel
Nowadays the importance of the peripheral blood stem cell (PBSC) donation is continuously increasing. It is therefore crucial to determine all side effects for donors. This clinical trial deals with the preventative therapy using Allopurinol (300 mg/d) in order to prohibit the increase in serum uric acid as a result of rhG-CSF injections (10 g/kg Lenograstim over 5d). It comprises 72 donors (m/f: 57/15) passing through 3 examinations. The initial measurement of uric acid serves as clue to categorize the donors into groups. Donors with high uric acid are summarized as group 1 (30: m/f: 27/3), which obtained Allopurinol, whereas donors with normal uric acid are part of group 4 (42: m/f: 30/12). This group is additionally divided into groups 2 and 3 (dependent on the way of G-CSF administration). The mean serum uric acid of group 1 decreased from 7.01 to 5.03 mg/dl. In contrast, the value of group 4 increased from 5.16 to 6.15 mg/dl.
{"title":"Effect of the preventative allopurinol therapy within the framework of the peripheral blood stem cell donation","authors":"E. Müller, Miriam Hübner, S. Kuderer, Marie Prechtl, J. Rox, J. Fischer, F. Wenzel","doi":"10.3233/JCB-179013","DOIUrl":"https://doi.org/10.3233/JCB-179013","url":null,"abstract":"Nowadays the importance of the peripheral blood stem cell (PBSC) donation is continuously increasing. It is therefore crucial to determine all side effects for donors. This clinical trial deals with the preventative therapy using Allopurinol (300 mg/d) in order to prohibit the increase in serum uric acid as a result of rhG-CSF injections (10 g/kg Lenograstim over 5d). It comprises 72 donors (m/f: 57/15) passing through 3 examinations. The initial measurement of uric acid serves as clue to categorize the donors into groups. Donors with high uric acid are summarized as group 1 (30: m/f: 27/3), which obtained Allopurinol, whereas donors with normal uric acid are part of group 4 (42: m/f: 30/12). This group is additionally divided into groups 2 and 3 (dependent on the way of G-CSF administration). The mean serum uric acid of group 1 decreased from 7.01 to 5.03 mg/dl. In contrast, the value of group 4 increased from 5.16 to 6.15 mg/dl.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-179013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42341951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ann-Kathrin Niebergall, S. Pfeiffer, C. Gruber, H. Sievers, A. Nies, A. Kehlen, B. Hiebl
{"title":"Influence of polymerized siloxane coating on growth and biofilm formation of aerobic grown nosocomial bacteria","authors":"Ann-Kathrin Niebergall, S. Pfeiffer, C. Gruber, H. Sievers, A. Nies, A. Kehlen, B. Hiebl","doi":"10.3233/JCB-179010","DOIUrl":"https://doi.org/10.3233/JCB-179010","url":null,"abstract":"","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-179010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41940928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Drug induced liver injury (DILI) is still the leading single cause of drug failure during clinical phases and after market approval. Currently, many laboratories aim to develop appropriate in vitro systems to predict drug hepatotoxicity. Primary human hepatocytes are still the gold standard, but they have substantial disadvantages such as rapid dedifferentiation in vitro and lack of cell proliferation. In addition to primary human hepatocytes, liver cancer-derived cell lines such as HepG2, cytochrome P450 (CYP450) overexpressing HepG2 cell clones and HepaRG were studied intensively. In contrast to HepG2, HepaRG show promising characteristics of differentiated primary human hepatocytes, but they represent only one donor. There is some hope that this lack of donor variability can be solved by the use of iPS-derived hepatocytes. However, iPS technology still seems to need some improvement to produce physiologically relevant hepatocytes. Upcyte hepatocytes represent the most recent technical advancement combining some features of primary human hepatocytes such as physiological activity and donor variability with the ability of cell lines for extended proliferation. Altogether, more work is needed to develop and validate appropriate in vitro systems for precise prediction of DILI risk.
{"title":"Human hepatocyte systems for in vitro toxicology analysis","authors":"S. Kammerer, J. Küpper","doi":"10.3233/JCB-179012","DOIUrl":"https://doi.org/10.3233/JCB-179012","url":null,"abstract":"Drug induced liver injury (DILI) is still the leading single cause of drug failure during clinical phases and after market approval. Currently, many laboratories aim to develop appropriate in vitro systems to predict drug hepatotoxicity. Primary human hepatocytes are still the gold standard, but they have substantial disadvantages such as rapid dedifferentiation in vitro and lack of cell proliferation. In addition to primary human hepatocytes, liver cancer-derived cell lines such as HepG2, cytochrome P450 (CYP450) overexpressing HepG2 cell clones and HepaRG were studied intensively. In contrast to HepG2, HepaRG show promising characteristics of differentiated primary human hepatocytes, but they represent only one donor. There is some hope that this lack of donor variability can be solved by the use of iPS-derived hepatocytes. However, iPS technology still seems to need some improvement to produce physiologically relevant hepatocytes. Upcyte hepatocytes represent the most recent technical advancement combining some features of primary human hepatocytes such as physiological activity and donor variability with the ability of cell lines for extended proliferation. Altogether, more work is needed to develop and validate appropriate in vitro systems for precise prediction of DILI risk.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-179012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49273795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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