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Hydrogen Sulfide in the Cardiovascular System: A Small Molecule with Promising Therapeutic Potential 心血管系统中的硫化氢:一种具有良好治疗潜力的小分子
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-11 DOI: 10.20944/PREPRINTS202101.0193.V1
I. Tikhomirova, A. Muravyov
this review summarizes current knowledge of the hydrogen sulfide role in cardiovascular system, the proposed mechanisms of its action and the prospects for its applicability in the treatment of cardiovascular diseases. Hydrogen sulfide was recently recognized as gasotransmitter – simple signaling molecule which freely penetrates the cell membrane and regulates a number of biological functions. In humans endogenous H2S is generated via enzymatic and non-enzymatic pathways and its content varies in different tissues and is strictly regulated. In cardiovascular system H2S is produced by myocardial, vascular and blood cells and regulates a number of vital functions. Numerous experimental data prove that endogenously generated as well as exogenously administered H2S exerts a wide range of actions in cardiovascular system, including vasodilator/vasoconstrictor effects, regulation of blood pressure, pro-apoptotic and anti-proliferative effects in the vascular smooth muscle cells, influence on angiogenesis and erythropoiesis, myocardial cytoprotection in ischemia-reperfusion injury, oxygen sensing, inhibition of platelet aggregation and blood coagulation, modification of erythrocyte microrheological properties (aggregability and deformability). Understanding of molecular mechanisms of H2S action and molecular crosstalk between H2S, NO, and CO is essential for the development of its diagnostic and therapeutic potential.
本文综述了硫化氢在心血管系统中的作用,提出了其作用机制,并对其在心血管疾病治疗中的应用前景进行了展望。硫化氢最近被认为是一种气体传输剂——一种简单的信号分子,可以自由穿透细胞膜并调节多种生物功能。在人类中,内源性H2S通过酶和非酶途径产生,其含量在不同组织中不同,并受到严格调控。在心血管系统中,H2S由心肌、血管和血细胞产生,并调节许多重要功能。大量实验数据证明,内源性和外源性给药的H2S在心血管系统中发挥广泛的作用,包括血管舒张/血管收缩作用、血压调节、血管平滑肌细胞中的促凋亡和抗增殖作用、对血管生成和红细胞生成的影响,缺血再灌注损伤中的心肌细胞保护、氧感应、抑制血小板聚集和凝血、改变红细胞微流变特性(聚集性和变形性)。了解H2S作用的分子机制以及H2S、NO和CO之间的分子串扰对于开发其诊断和治疗潜力至关重要。
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引用次数: 0
Comparative efficiency of three gasotransmitters (nitric oxide, hydrogen sulfide and carbon monoxide): Analysis on the model of red blood cell microrheological responses 三种气体传递器(一氧化氮、硫化氢和一氧化碳)的比较效率:红细胞微流变反应模型分析
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.3233/jcb-200023
A. V. Muravyov, I. Tikhomirova, P. Avdonin, S. Bulaeva, J. Malisheva
BACKGROUND: It is now known regulatory effect of gaseous mediators in many bodily functions. These mediators include nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S). However, detailed data on the regulatory role of each of these gasotransmitters (GTs) are still not sufficiently studied. OBJECTIVE: The aim of this study was to investigate on models of microrheological behaviour of intact red blood cells and their recovered ghosts of cellular responses to all three known gasotransmitters: NO, H2S, and CO. METHODS: In experiments with intact red blood cells (RBCs) and their recovered ghosts (filled with an isotonic solution of known viscosity), deformability (RBCD) and aggregation (RBCA) were recorded after incubation of cells with GT donors or stimulators of their endogenous synthesis. RESULTS: It was found that all three GT donors moderately increased the deformability of both intact RBCs and their recovered ghosts (by 5–10%, p < 0.05). In addition GT donors and substrates of their endogenous synthesis significantly reduced RBCA, from 20 to 37% (p < 0.01). Experiments with inhibition of soluble guanylate cyclase (s-GC) almost completely eliminated the RBCD increasing effect of GT donors CONCLUSION: In present study it was demonstrated that all three known gaseous mediators: NO, CO and H2S cause moderate statistically significant positive alterations in RBC deformability as well as a noticeable decrease in RBC aggregation. This was confirmed both in experiments with gasotransmitter donors and stimulators of GT endogenous synthesis. In addition, the data obtained suggest that RBC microrheological responses to the actions of all three GTs are associated with activation of the guanylate cyclase signalling cascade.
背景:目前已知气体介质在许多身体功能中的调节作用。这些介质包括一氧化氮(NO)、一氧化碳(CO)和硫化氢(H2S)。然而,关于这些气体递质(GTs)的调节作用的详细数据仍然没有得到充分的研究。目的:本研究的目的是研究完整红细胞的微流变行为模型及其对所有三种已知气体递质(NO, H2S和CO)的细胞反应的恢复鬼。在完整红细胞(rbc)及其恢复的鬼(充满已知粘度的等渗溶液)的实验中,在细胞与GT供体或其内源性合成刺激物孵育后,记录了细胞的可变形性(RBCD)和聚集性(RBCA)。结果:三种GT供体均能适度提高完整红细胞及其恢复后的残余红细胞的变形能力(5% ~ 10%,p < 0.05)。此外,GT供体及其内源性合成底物显著降低RBCA,从20%降至37% (p < 0.01)。抑制可溶性鸟苷酸环化酶(s-GC)的实验几乎完全消除了GT供体增加RBCD的作用。结论:本研究表明,所有三种已知的气体介质:NO, CO和H2S都能引起RBC变形性的中度统计学意义上的积极改变,以及RBC聚集性的显著降低。这在气体传递素供体和刺激剂的实验中都得到了证实。此外,获得的数据表明,红细胞对所有三种gt作用的微流变反应与鸟苷酸环化酶信号级联的激活有关。
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引用次数: 2
Tissue engineering in periodontics- A demystifying review 牙周病的组织工程-揭秘综述
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.3233/jcb-200028
Shivani Sachdeva, H. Saluja, Amit Mani, M. Phadnaik, U. Palekar
INTRODUCTION: Novel concept known as tissue engineering is for the betterment of human. The use of much advanced molecular science and cell biology in processing the tissues to regenerate even after the loss of inborn tendency of pluripotent cells to multiply is possible by this new therapy. CONTENT: Periodontal tissue regeneration in both height and function is attributed to a complete recovery of the periodontal structures, that is, the formation of alveolar bone, a new connective attachment through collagen fibers as well as functionally oriented on the newly formed cementum is regeneration. Cell based therapies including tissue regeneration is an alternative approach for the regeneration of tissues damaged by disease or trauma. SUMMARY: Though tissue engineering requires the fundamentals of all the three keys namely genomics, proteomics and biometrics to give the solutions to biological problems appearing in dentistry as well as medical sciences.
引言:组织工程是一种新的概念,旨在改善人类的健康。利用先进的分子科学和细胞生物学来处理组织再生,即使在多能细胞天生的繁殖倾向丧失之后,这种新疗法也成为可能。内容:牙周组织再生在高度和功能上都归因于牙周结构的完全恢复,即牙槽骨的形成,通过胶原纤维形成新的结缔组织附着体以及在功能上定向于新形成的牙骨质的再生。包括组织再生在内的细胞疗法是一种再生因疾病或创伤而受损的组织的替代方法。摘要:虽然组织工程需要基因组学、蛋白质组学和生物计量学这三个关键的基础知识来解决牙科和医学科学中出现的生物学问题。
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引用次数: 2
Practical evaluation of two commercial immunoassays for the quantification of steroid hormones in human saliva 两种商业免疫测定法定量人唾液中类固醇激素的实际评价
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.3233/jcb-209012
Jonas Schmidt, F. Wenzel, F. Blessing
BACKGROUND: There are certain issues using saliva as a sample for steroid hormone analysis in clinical diagnostics. Therefore, it is important to test whether currently available assays are suitable for this approach in a routine laboratory. OBJECTIVES: The analytical performance of two commercially available immunoassays was compared under routine conditions. In addition, salivary steroid hormone levels were compared with serum levels. METHODS: Saliva and serum samples were collected from healthy subjects. Afterwards, the concentration of cortisol, testosterone, progesterone and estradiol in saliva was measured by using the ADIVA Centaur® XP platform and commercially available manual ELISA kits. Serum levels of the selected steroid hormones were determined by using the ADVIA Centaur® platform. RESULTS: Currently, the ADVIA Centaur® XP platform cannot be used for the quantification of steroid hormones in saliva due to an insufficient calibration range. Commercially available ELISA kits on the other side are well suitable for this application. CONCLUSIONS: There are commercially available immunoassays which are sensitive enough for the determination of steroid hormones from saliva and can be used under routine conditions. However, the interpretation of salivary steroid hormone levels stays a challenging task due to the large variability of the results.
背景:在临床诊断中使用唾液作为样本进行类固醇激素分析存在一定的问题。因此,在常规实验室中测试当前可用的分析方法是否适用于这种方法是很重要的。目的:比较两种市售免疫测定法在常规条件下的分析性能。此外,将唾液类固醇激素水平与血清水平进行比较。方法:采集健康受试者的唾液和血清。随后,使用ADIVA Centaur®XP平台和市售手动ELISA试剂盒检测唾液中皮质醇、睾酮、孕酮和雌二醇的浓度。使用ADVIA Centaur®平台测定所选类固醇激素的血清水平。结果:目前,由于校准范围不足,ADVIA Centaur®XP平台不能用于唾液中类固醇激素的定量。另一方面,市售的ELISA试剂盒非常适合这种应用。结论:目前市售的免疫检测方法具有足够的灵敏度,可用于唾液中类固醇激素的检测,且可在常规条件下使用。然而,由于结果的巨大可变性,唾液类固醇激素水平的解释仍然是一项具有挑战性的任务。
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引用次数: 0
Immunomodulatory response and serum level of neutrophil gelatinase associated lipocalin (NGAL) as a marker of acute kidney injury in wistar rats exposed to pyrethroids 中性粒细胞明胶酶相关脂钙蛋白(NGAL)作为拟除虫菊酯暴露的wistar大鼠急性肾损伤标志物的免疫调节反应和血清水平
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2021-01-01 DOI: 10.3233/jcb-210038
A. Atere, Olumide Faith Ajani, Akinpelu Moronkeji, Victory Oluwaseun Ajibade, H. Osadolor
BACKGROUND: Insecticide usage has increased in the tropics and subtropics due to the high prevalence of vector-borne infections, even though insecticide use effectively reduces insect-borne diseases. Insecticide exposure can cause oxidative stress and have severe consequences for human health. The study was then designed to evaluate oxidative stress and its effects on immunomodulatory and renal integrity among Wistar rats exposed to pyrethroids. METHODS: Eighty-four Wistar rats were randomly selected and divided into two groups. Fifty-one rats were exposed to 1.2 %w/v pyrethroids, while the remaining thirty-three rats were grouped as non-exposed. The groups were divided into three different groups, each with 7, 21, and 41 days. After days of exposure, the animals in each group were anesthetized, and blood samples were collected from the inferior vena cava. Using standard spectrophotometric techniques, the levels of total antioxidant status (TAS), malondialdehyde (MDA), glutathione (GSH), hydrogen peroxide (H2O2), urea, creatinine and uric acid were determined. Blood activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) were determined. ELISA was used to determine levels of IgG, IgA, IgE, TNF-α, and NGAL. Data obtained were statistically compared. RESULTS: The serum mean levels of SOD, GPx, CAT, GSH, and TAS were significantly reduced (p <  0.05) while mean levels of MDA, H2O2, IgG, IgE, IgA, TNFα, neutrophil gelatinase-associated lipocalin (NGAL), urea, uric acid, and creatinine were significantly elevated (p <  0.05) from 7 to 41 days exposure in exposed groups. NGAL had a higher area under the ROC curve than urea and creatinine. CONCLUSIONS: This study observed that pyrethroids can cause oxidative stress, deplete antioxidant levels, nephrotoxicity, and may modulate both humoral and cellular immune functions. It also established NGAL as a sensitive diagnostic tool and early biomarker for acute kidney injury (AKI).
背景:尽管杀虫剂的使用有效地减少了虫媒疾病,但由于病媒传播感染的高发,热带和亚热带地区的杀虫剂使用量有所增加。接触杀虫剂可引起氧化应激,对人体健康造成严重后果。该研究旨在评估氧化应激及其对接触拟除虫菊酯的Wistar大鼠免疫调节和肾脏完整性的影响。方法:随机选取Wistar大鼠84只,分为两组。51只大鼠暴露于1.2% w/v拟除虫菊酯,其余33只大鼠未暴露。这些组被分为三个不同的组,每个组分别有7天、21天和41天。暴露数日后,各组动物麻醉,下腔静脉采血。采用标准分光光度法测定总抗氧化状态(TAS)、丙二醛(MDA)、谷胱甘肽(GSH)、过氧化氢(H2O2)、尿素、肌酐和尿酸水平。测定血中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)、过氧化氢酶(CAT)的活性。ELISA法检测IgG、IgA、IgE、TNF-α、NGAL水平。所得资料进行统计学比较。结果:暴露7 ~ 41 d时,血清SOD、GPx、CAT、GSH、TAS均值显著降低(p < 0.05), MDA、H2O2、IgG、IgE、IgA、TNFα、中性粒细胞明胶酶相关脂钙蛋白(NGAL)、尿素、尿酸、肌酐均值显著升高(p < 0.05)。NGAL在ROC曲线下的面积高于尿素和肌酐。结论:本研究发现拟除虫菊酯可引起氧化应激、降低抗氧化水平、肾毒性,并可能调节体液和细胞免疫功能。该研究还确立了NGAL作为急性肾损伤(AKI)的敏感诊断工具和早期生物标志物的地位。
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引用次数: 0
Convalescent plasma therapy: A promising solution for SARS-CoV-2 outbreak 恢复期血浆治疗:SARS-CoV-2爆发的一个有希望的解决方案
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2020-12-19 DOI: 10.3233/jcb-200024
A. Singh, A. Chaterjee, S. Sirohi, Nikhil Sharma, A. Kathuria
Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has caused more than 18 million confirmed cases and 700000 deaths worldwide after the outbreak in November 2019 (COVID-19). It has been considered as most pathogenic infection under this category. Till date none of the therapeutics or prophylaxis measures have been claimed by any researcher which could cure the patient suffering from the SARS-CoV-2 infection. There is an urgent need for any alternative and effective way of disease management for COVID-19. However, convalescent plasma therapy (CPT) has gain attention of researchers with significant improvement of patient survival rate. Interestingly, there are numerous examples where CPT has proven its potential upon post-exposure prophylaxis and/or treatment in various diseases including COVID-19. This review summarizes the essential elements related to CPT, its past clinical evidences and application of CPT for the management of SARS-CoV-2 infection during COVID-19. We used the published literatures from PubMed, EMBASE and Medline databases until 31st May 2020.
自2019年11月爆发(新冠肺炎)以来,严重急性呼吸综合征冠状病毒-2(SARS-CoV-2)已在全球范围内造成超过1800万例确诊病例和70万人死亡。它被认为是这一类别下最具致病性的感染。迄今为止,没有任何研究人员声称有任何治疗或预防措施可以治愈严重急性呼吸系统综合征冠状病毒2型感染的患者。迫切需要任何替代和有效的新冠肺炎疾病管理方法。然而,随着患者生存率的显著提高,恢复期血浆治疗(CPT)引起了研究人员的关注。有趣的是,有许多例子表明,CPT已证明其在包括新冠肺炎在内的各种疾病的暴露后预防和/或治疗中的潜力。本文综述了CPT的基本要素、其过去的临床证据以及CPT在新冠肺炎期间管理SARS-CoV-2感染中的应用。截至2020年5月31日,我们使用了PubMed、EMBASE和Medline数据库中已发表的文献。
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引用次数: 1
Whole blood based cytokine release assay assesses cellular immunity in hepatitis E resolver patients 全血细胞因子释放试验评估戊型肝炎消退患者的细胞免疫
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-16 DOI: 10.3233/JCB-189007
W. Dammermann, K. Bröker, F. Bentzien, K. Börner, Katharina Heinzel, S. Lüth
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引用次数: 0
Immersion technique for the preparation of protein gradients on Collagen Type I membranes to control cell migration 在I型胶原膜上制备蛋白质梯度以控制细胞迁移的浸没技术
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-16 DOI: 10.3233/JCB-180012
S. Berger, Jan Kiebist, Elisabeth Pötschke, K. Salchert
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引用次数: 0
Tyramide signal amplification as universal detection method on protein coated microbeads 酰胺信号放大作为蛋白包被微珠的通用检测方法
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-16 DOI: 10.3233/JCB-189003
Christoph Jurischka, F. Dinter, M. Sowa, J. Noack, Juliane Schiebel, D. Roggenbuck, P. Schierack, S. Rödiger
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引用次数: 2
Photoacids in biochemical applications 光酸在生物化学中的应用
Q3 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-16 DOI: 10.3233/JCB-189004
H. Kagel, M. Frohme, J. Glökler
BACKGROUND: After excitation with light photoacids can change the pH in a solution by release of a proton. They have been used mostly for excited state proton transfer studies. In this review the general functionality and mechanisms and the subdivision of photoacids is explained. STATE OF THE ART: Different uses of photoacids are described, covering a wide range of various biochemical topics, focusing on biochemical applications. Examples for the introduced subdivisions are covered. CONCLUSIONS AND OUTLOOK: The areas in which photoacids can be employed are diverse. Photoacids have a promising future in biotechnology and biochemistry and should be considered for upcoming applications, especially in non-invasive control of biochemical reactions.
背景:在用光激发后,光酸可以通过释放质子来改变溶液中的pH。它们主要用于激发态质子转移研究。在这篇综述中,解释了光酸的一般功能、机制和细分。最新技术:描述了光酸的不同用途,涵盖了广泛的各种生物化学主题,重点是生物化学应用。介绍了引入的细分的示例。结论和展望:光酸可用于的领域多种多样。光酸在生物技术和生物化学中有着广阔的前景,应考虑在即将到来的应用中,特别是在生物化学反应的非侵入性控制中。
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引用次数: 9
期刊
Journal of Cellular Biotechnology
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