Pub Date : 2021-01-01DOI: 10.15584/EJCEM.2021.1.11
Berrin Erok, K. Kıbıcı
Introduction. Rhabdomyosarcoma (RMS) is the most common pediatric soft tissue sarcoma with 10 % of the cases occuring in the orbit. Patients often present with a rapidly developing proptosis and globe displacement. Aim. We aimed to present a very rare presentation of orbital RMS, with a giant exophytic orbital mass, a very rare presentation occuring in more advanced cases. Description of the case. A 3-year old girl presented to our hospital with a rapidly enlarging tissue like ulcerative mass. Her past medical history was remarkable with the diagnosis of embryonal rhabdomyosarcoma (RMS) and treatment with chemoradiotherapy at the age of 15 months. On magnetic resonance imaging (MRI), there was a giant heterogenously enhancing mass filling the right orbit and extending to the intracranial region. Li Fraumeni syndrome (LFS) was considered due to her sister death from neuroblastoma at an early age. Cytogenetic analysis revealed mutations of p53 gene, which supported our consideration. Conclusion. RMS is a highly malignant tumor which usually occurs sporadiacally. However, some rare syndromes are associated with increased incidence of RMS, such as LFS.
{"title":"An aggressive locoregional orbital rhabdomyosarcoma and Li Fraumeni syndrome","authors":"Berrin Erok, K. Kıbıcı","doi":"10.15584/EJCEM.2021.1.11","DOIUrl":"https://doi.org/10.15584/EJCEM.2021.1.11","url":null,"abstract":"Introduction. Rhabdomyosarcoma (RMS) is the most common pediatric soft tissue sarcoma with 10 % of the cases occuring in the orbit. Patients often present with a rapidly developing proptosis and globe displacement. Aim. We aimed to present a very rare presentation of orbital RMS, with a giant exophytic orbital mass, a very rare presentation occuring in more advanced cases. Description of the case. A 3-year old girl presented to our hospital with a rapidly enlarging tissue like ulcerative mass. Her past medical history was remarkable with the diagnosis of embryonal rhabdomyosarcoma (RMS) and treatment with chemoradiotherapy at the age of 15 months. On magnetic resonance imaging (MRI), there was a giant heterogenously enhancing mass filling the right orbit and extending to the intracranial region. Li Fraumeni syndrome (LFS) was considered due to her sister death from neuroblastoma at an early age. Cytogenetic analysis revealed mutations of p53 gene, which supported our consideration. Conclusion. RMS is a highly malignant tumor which usually occurs sporadiacally. However, some rare syndromes are associated with increased incidence of RMS, such as LFS.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78235018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.15584/EJCEM.2021.1.13
A. Pliszka, M. Klos, Klaudia Możdżeń, Krzysztof Balawender
Introduction. Variations in the urogenital vascular anomalies in the abdomen are common. However, they warrant attention due to their importance in operative, diagnostic, and endovascular procedures. Aim. The aim of this article is to show an example of a patient with rare kidney and vessels anomalies. We want to prove that those anomalies contributed to development of urolithiasis in this case. Description of the case. During dissection of abdomen in a female cadaver, unique vascular anomalies and a position disorder of both kidneys were observed. Conclusion. Vessel abnormalities were congenital and appeared simultaneously with renal dystopia. Both anomalies could contribute to stone formation and nephritis. Understanding of the urogenital anatomical variations and their relations to adjacent structures is significant during surgical and radiological procedures.
{"title":"Urolithiasis due to renal dystopia and vascular anomalies","authors":"A. Pliszka, M. Klos, Klaudia Możdżeń, Krzysztof Balawender","doi":"10.15584/EJCEM.2021.1.13","DOIUrl":"https://doi.org/10.15584/EJCEM.2021.1.13","url":null,"abstract":"Introduction. Variations in the urogenital vascular anomalies in the abdomen are common. However, they warrant attention due to their importance in operative, diagnostic, and endovascular procedures. Aim. The aim of this article is to show an example of a patient with rare kidney and vessels anomalies. We want to prove that those anomalies contributed to development of urolithiasis in this case. Description of the case. During dissection of abdomen in a female cadaver, unique vascular anomalies and a position disorder of both kidneys were observed. Conclusion. Vessel abnormalities were congenital and appeared simultaneously with renal dystopia. Both anomalies could contribute to stone formation and nephritis. Understanding of the urogenital anatomical variations and their relations to adjacent structures is significant during surgical and radiological procedures.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86326624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.15584/EJCEM.2020.4.13
Joanna Gustalik, D. Aebisher, E. Kaznowska, D. Bartusik-Aebisher
Introduction. Mixed adenocarcinoma-neuroendocrine cancer (MANEC) is a rare cancer that is characterized by aggressive course and poor prognosis. Aim. A case report and literature review. Description of the case. This article presents the case of a 63-year-old patient who was hospitalized due to the occurrence of neurological symptoms such as nausea, dizziness and headache as well as double vision and numbness of the hands. Suspected ischemic stroke, meningitis with bacterial etiology or brainstem pathology. Conclusion. MANEC composed of large neuroendocrine cells have better survival and clinical behavior than patients with small, intermediate or mixed large and intermediate cells.
{"title":"Mixed adenocarcinoma-neuroendocrine cancer – a case report","authors":"Joanna Gustalik, D. Aebisher, E. Kaznowska, D. Bartusik-Aebisher","doi":"10.15584/EJCEM.2020.4.13","DOIUrl":"https://doi.org/10.15584/EJCEM.2020.4.13","url":null,"abstract":"Introduction. Mixed adenocarcinoma-neuroendocrine cancer (MANEC) is a rare cancer that is characterized by aggressive course and poor prognosis. Aim. A case report and literature review. Description of the case. This article presents the case of a 63-year-old patient who was hospitalized due to the occurrence of neurological symptoms such as nausea, dizziness and headache as well as double vision and numbness of the hands. Suspected ischemic stroke, meningitis with bacterial etiology or brainstem pathology. Conclusion. MANEC composed of large neuroendocrine cells have better survival and clinical behavior than patients with small, intermediate or mixed large and intermediate cells.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79720555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.15584/EJCEM.2021.1.15
M. Osuchowski, D. Bartusik-Aebisher, E. Kaznowska, D. Aebisher
Introduction. Metaplastic breast carcinoma is expressing epithelial and/or mesenchymal tissue within the same tumor. Aim. The aim of this study is to evaluate metaplastic breast carcinoma in a case report and literature review. Description of the case. The presented case desribes metaplastic carcinoma of the breast in 65 years old female patient. Conclusion. Fibromatosis-cell metaplastic carcinoma of the breast presents a particularly large diagnostic challenge. Malignant variants of this disease have been described in the literature.
{"title":"Fibromatosis-like spindle-cell metaplastic carcinoma of the breast – a case report","authors":"M. Osuchowski, D. Bartusik-Aebisher, E. Kaznowska, D. Aebisher","doi":"10.15584/EJCEM.2021.1.15","DOIUrl":"https://doi.org/10.15584/EJCEM.2021.1.15","url":null,"abstract":"Introduction. Metaplastic breast carcinoma is expressing epithelial and/or mesenchymal tissue within the same tumor. Aim. The aim of this study is to evaluate metaplastic breast carcinoma in a case report and literature review. Description of the case. The presented case desribes metaplastic carcinoma of the breast in 65 years old female patient. Conclusion. Fibromatosis-cell metaplastic carcinoma of the breast presents a particularly large diagnostic challenge. Malignant variants of this disease have been described in the literature.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74707213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.15584/EJCEM.2020.4.11
Piotr Przyczyna, Elżbieta Trojnar, D. Bartusik-Aebisher, D. Aebisher
Introduction. Gastrointestinal stromal tumor (GIST) is most often locate in the region of the stomach and the proximal part of the small intestine. Aim. The multiple histopathological examination is described. Description of the case. Multiple GISTs are rare neoplasms that originate from the interstitial cells are described. Conclussion. GIST can occur in any part of the gut, they are most common in the stomach and small intestine, and less frequent in the colorectum and esophagus. Although their pathogenesis and clinical manifestations are different, these tumor syndromes confer a high risk for developing multiple neoplasms.
{"title":"Numerous gastrointestinal tumors","authors":"Piotr Przyczyna, Elżbieta Trojnar, D. Bartusik-Aebisher, D. Aebisher","doi":"10.15584/EJCEM.2020.4.11","DOIUrl":"https://doi.org/10.15584/EJCEM.2020.4.11","url":null,"abstract":"Introduction. Gastrointestinal stromal tumor (GIST) is most often locate in the region of the stomach and the proximal part of the small intestine. Aim. The multiple histopathological examination is described. Description of the case. Multiple GISTs are rare neoplasms that originate from the interstitial cells are described. Conclussion. GIST can occur in any part of the gut, they are most common in the stomach and small intestine, and less frequent in the colorectum and esophagus. Although their pathogenesis and clinical manifestations are different, these tumor syndromes confer a high risk for developing multiple neoplasms.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90941053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction. Estrogen receptors (ER) are members of nuclear receptors that act in the ER signaling pathway regulating the pathophysiology of hormone-responsive target cells including breast tissue. Aim. This detailled review literature was written on the pathophysiology of ER signaling as well as the effect altered ERα and associated pathway derangement in the oncogenesis of breast cancer. Material and methods. This review was performed according to systematic literature search of three major bibliographic databases (Scopus, PubMed, and Cochran). Analysis of the literature. In this pathway, estrogen receptor alpha (ERα) is a key estradiol-17β (E2) induced transcription factor that has been implicated in the initiation and development of the major fraction of breast cancers. Hence understanding the ERα-mediated ER signaling that results in alterations from normal phenotypic features of breast tissue to the oncogenic features of breast cancer is important. The oncogenic effect of ERα in ER signaling is driven by combinations of molecular assets within the cancer cells. Normally, the transcriptional activity of ERα is controlled by tight regulation of its protein level inside the cells. Altered stability and activity of ERα due to its phosphorylation, ubiquitination, glycosylation, sumoylation, and acetylation events can trigger oncogenic ER signaling. Conclusion. The function and activity of ERα is also modulated by its interaction with coregulators as well as crosstalk with oncogenic factors from other oncogenic pathways. These all events increase the complexity of the progression of ER+ breast cancer and its response to endocrine therapy.
{"title":"Pathophysiological roles of ERα in the ER signaling mediated oncogenesis of breast cancer","authors":"Sultan Abda Neja","doi":"10.15584/EJCEM.2020.4.6","DOIUrl":"https://doi.org/10.15584/EJCEM.2020.4.6","url":null,"abstract":"Introduction. Estrogen receptors (ER) are members of nuclear receptors that act in the ER signaling pathway regulating the pathophysiology of hormone-responsive target cells including breast tissue. Aim. This detailled review literature was written on the pathophysiology of ER signaling as well as the effect altered ERα and associated pathway derangement in the oncogenesis of breast cancer. Material and methods. This review was performed according to systematic literature search of three major bibliographic databases (Scopus, PubMed, and Cochran). Analysis of the literature. In this pathway, estrogen receptor alpha (ERα) is a key estradiol-17β (E2) induced transcription factor that has been implicated in the initiation and development of the major fraction of breast cancers. Hence understanding the ERα-mediated ER signaling that results in alterations from normal phenotypic features of breast tissue to the oncogenic features of breast cancer is important. The oncogenic effect of ERα in ER signaling is driven by combinations of molecular assets within the cancer cells. Normally, the transcriptional activity of ERα is controlled by tight regulation of its protein level inside the cells. Altered stability and activity of ERα due to its phosphorylation, ubiquitination, glycosylation, sumoylation, and acetylation events can trigger oncogenic ER signaling. Conclusion. The function and activity of ERα is also modulated by its interaction with coregulators as well as crosstalk with oncogenic factors from other oncogenic pathways. These all events increase the complexity of the progression of ER+ breast cancer and its response to endocrine therapy.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88275296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yaprak Donmez Cakil, Z. Ozunal, D. Kayalı, R. Aktas
Introduction. Cancer is a leading cause of mortality. Hepatocellular cancer is one of the malignancies associated with poor outcome and resistance to pharmacotherapy. Cancer stem cells (CSCs) contribute to resistance to therapy and hence lead to the treatment failure of tumors. Aim. This study aims to explore the expression of CSCs in response to cisplatin treatment in HepG2 hepatocellular cancer cell line. Material and methods. Cell proliferation test, CCK-8, was used to evaluate the cell proliferation following cisplatin treatment for 72 hours. The expressions of CSC markers CD44, CD90, and CD133 were assessed by flow cytometric analysis. Results. The results showed a dose-dependent decrease in cell proliferation and increased expression of CSC markers CD44 and CD90 in response to cisplatin. Conclusion. Understanding the roles of CSC markers may point to new targets and therapeutic strategies to predict and overcome cisplatin resistance.
{"title":"The expression of CD44, CD90 and CD133 in response to cisplatin in hepatocellular cancer cells","authors":"Yaprak Donmez Cakil, Z. Ozunal, D. Kayalı, R. Aktas","doi":"10.15584/EJCEM.2021.1.3","DOIUrl":"https://doi.org/10.15584/EJCEM.2021.1.3","url":null,"abstract":"Introduction. Cancer is a leading cause of mortality. Hepatocellular cancer is one of the malignancies associated with poor outcome and resistance to pharmacotherapy. Cancer stem cells (CSCs) contribute to resistance to therapy and hence lead to the treatment failure of tumors. Aim. This study aims to explore the expression of CSCs in response to cisplatin treatment in HepG2 hepatocellular cancer cell line. Material and methods. Cell proliferation test, CCK-8, was used to evaluate the cell proliferation following cisplatin treatment for 72 hours. The expressions of CSC markers CD44, CD90, and CD133 were assessed by flow cytometric analysis. Results. The results showed a dose-dependent decrease in cell proliferation and increased expression of CSC markers CD44 and CD90 in response to cisplatin. Conclusion. Understanding the roles of CSC markers may point to new targets and therapeutic strategies to predict and overcome cisplatin resistance.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86600945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Olaniyi, G. J. Emeka, A. Onifade, A. Adeyanju, S. Rahamon
Introduction. Reports have shown that there is a rise in beta-2-microglobulin (β2M) concentration in patients with HIV infection and that the degree of elevation correlates well with the extent of disease burden and could be an independent prognostic marker for death. However, there is the dearth of information on the interplay between alteration in haematological profile, a common cause of morbidity and mortality in HIV, and β2M. Aim. Changes in selected haematological parameters and β2M in Nigerian HIV patients stratified based on CD4+ T-cells counts were thus assessed in this study. Material and methods. Forty-eight asymptomatic, drug naive HIV patients were enrolled into this cross-sectional study. Haemoglobin concentration (Hb), packed cell volume (PCV), total and differential white blood cell count, platelet count and CD4+ T-cells count were determined using standard methods while serum levels of β2M were determined using ELISA. Thereafter, the patients were stratified into three groups based on the CD4+ T-cells count. Results. Hb and lymphocyte counts increased with increasing CD4+ T-cells count. In contrast, neutrophils percentage, MCV and MCH reduced with increasing CD4+ T-cells count. The mean lymphocytes percentage was significantly higher while the mean neutrophils percentage was significantly lower in patients with CD4+ T-cells count of 500–800 cells/μl compared with the patients with CD4+ T-cells count <200 cells/μl. Similarly, the mean MCV was significantly lower in patients with CD4+ T-cells count of 500–800 cells/μl compared with patients with CD4+ T-cells count of 200–499 cells/μl and patients with CD4+ T-cells count <200 cells/μl. β2M had significant positive correlation with WBC and neutrophils percentage but had a significant negative correlation with lymphocytes percentage and MCH in patients with CD4+ T-cells count <200 cells/μl. However, β2M had significant positive correlation with PCV, Hb, monocytes and morphology in patients with CD4+ T-cells count of 500–800 cells/μl. Conclusion. It could be concluded from this study that HIV infection is associated with alteration in haematological profile and the alteration is CD4+ T-cells count-dependent. Also, elevation in β2M concentration appears to be a marker of lymphopaenia in patients with low CD4+ T-cells count.
{"title":"Changes in haematological parameters and serum beta-2-microglobulin levels in CD4+ T-cells-stratified Nigerian HIV patients","authors":"J. Olaniyi, G. J. Emeka, A. Onifade, A. Adeyanju, S. Rahamon","doi":"10.15584/EJCEM.2021.1.5","DOIUrl":"https://doi.org/10.15584/EJCEM.2021.1.5","url":null,"abstract":"Introduction. Reports have shown that there is a rise in beta-2-microglobulin (β2M) concentration in patients with HIV infection and that the degree of elevation correlates well with the extent of disease burden and could be an independent prognostic marker for death. However, there is the dearth of information on the interplay between alteration in haematological profile, a common cause of morbidity and mortality in HIV, and β2M. Aim. Changes in selected haematological parameters and β2M in Nigerian HIV patients stratified based on CD4+ T-cells counts were thus assessed in this study. Material and methods. Forty-eight asymptomatic, drug naive HIV patients were enrolled into this cross-sectional study. Haemoglobin concentration (Hb), packed cell volume (PCV), total and differential white blood cell count, platelet count and CD4+ T-cells count were determined using standard methods while serum levels of β2M were determined using ELISA. Thereafter, the patients were stratified into three groups based on the CD4+ T-cells count. Results. Hb and lymphocyte counts increased with increasing CD4+ T-cells count. In contrast, neutrophils percentage, MCV and MCH reduced with increasing CD4+ T-cells count. The mean lymphocytes percentage was significantly higher while the mean neutrophils percentage was significantly lower in patients with CD4+ T-cells count of 500–800 cells/μl compared with the patients with CD4+ T-cells count <200 cells/μl. Similarly, the mean MCV was significantly lower in patients with CD4+ T-cells count of 500–800 cells/μl compared with patients with CD4+ T-cells count of 200–499 cells/μl and patients with CD4+ T-cells count <200 cells/μl. β2M had significant positive correlation with WBC and neutrophils percentage but had a significant negative correlation with lymphocytes percentage and MCH in patients with CD4+ T-cells count <200 cells/μl. However, β2M had significant positive correlation with PCV, Hb, monocytes and morphology in patients with CD4+ T-cells count of 500–800 cells/μl. Conclusion. It could be concluded from this study that HIV infection is associated with alteration in haematological profile and the alteration is CD4+ T-cells count-dependent. Also, elevation in β2M concentration appears to be a marker of lymphopaenia in patients with low CD4+ T-cells count.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86613270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction. Microtiter plate assay (MPA) remains one of workhorses of in vitro biofilm research but it requires optimization of experimental conditions to fulfill the biofilm formation requirements of different bacterial pathogens. Aim. The aim was to determine the effect of TSB and RPMI1640 culture media and selected culture variables (O2 vs. 5% CO2, extended incubation time) on the biofilm production by bacteria commonly involved in biofilm-related infections: Enterococcus faecalis (EF), Escherichia coli (EC), Staphylococcus aureus (SA), Pseudomonas aeruginosa (PA), Klebsiella pneumoniae (KP). Material and methods. The investigation was performed using the MPA with crystal violet. Results. Statistically significant (p<0.05) increase in biofilm production between 24h and 72h time points was observed for EF (TSB o2, RPMIo2 and RPMIco2), EC (TSBo2), SA (TSBo2, TSBco2), KP (TSBo2, TSBco2), PA (RPMIco2, TSBco2). The TSB caused a significantly greater stimulation of biofilm production compared to RPM1640. It outcompeted RPMI1640 irrespective of the atmospheric conditions for SA and KP and under aerobic conditions for EF. Conclusion. Although the TSB provided the most optimal conditions for biofilm production, the process was influenced by the strain type, atmospheric conditions and period of cultivation which limits the ability to design a single universal model of the in vitro biofilm investigation.
{"title":"Analysis of the bacterial biofilm formation in different models of the in vitro culture","authors":"Agnieszka Bogut, A. Magryś","doi":"10.15584/EJCEM.2021.1.6","DOIUrl":"https://doi.org/10.15584/EJCEM.2021.1.6","url":null,"abstract":"Introduction. Microtiter plate assay (MPA) remains one of workhorses of in vitro biofilm research but it requires optimization of experimental conditions to fulfill the biofilm formation requirements of different bacterial pathogens. Aim. The aim was to determine the effect of TSB and RPMI1640 culture media and selected culture variables (O2 vs. 5% CO2, extended incubation time) on the biofilm production by bacteria commonly involved in biofilm-related infections: Enterococcus faecalis (EF), Escherichia coli (EC), Staphylococcus aureus (SA), Pseudomonas aeruginosa (PA), Klebsiella pneumoniae (KP). Material and methods. The investigation was performed using the MPA with crystal violet. Results. Statistically significant (p<0.05) increase in biofilm production between 24h and 72h time points was observed for EF (TSB o2, RPMIo2 and RPMIco2), EC (TSBo2), SA (TSBo2, TSBco2), KP (TSBo2, TSBco2), PA (RPMIco2, TSBco2). The TSB caused a significantly greater stimulation of biofilm production compared to RPM1640. It outcompeted RPMI1640 irrespective of the atmospheric conditions for SA and KP and under aerobic conditions for EF. Conclusion. Although the TSB provided the most optimal conditions for biofilm production, the process was influenced by the strain type, atmospheric conditions and period of cultivation which limits the ability to design a single universal model of the in vitro biofilm investigation.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89684368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction. Lipid is an essential component of the cell and its organelles membrane. The uniqueness and selectivity of lipids to specific functions and asymmetry of lipid distribution in the organelle’s membrane give the cell ability of being highly qualified and specified. Aim. The paper provides a comprehensive review of membrane lipids in different tissues and organelles of the cell in norm and disease. Material and methods. The paper analyzed the present literature data on membrane lipids behavior in physiology and pathology. Analysis of the literature. The major structural and functional lipids of the cell membrane are phosphatidylcholine > phosphatidylethanolamine. The absence/deficiency or augmentation of a specific type of lipid results in serious defects and usually life-threatening with a permanent disability. The observations discussed here suggest, the lipid peroxidation severity depends on the membrane lipid composition of the cell. Some tissue cells can handle lipoperoxidation and protect themselves from the peroxidation damaging products better, while other cells cannot compensate. Therefore, some organs are highly sensitive to peroxidation and irreversible changes occur rapidly. Conclusion. To sum up, the understanding of lipid’s role in norm and disease is clinically crucial to evaluate a novel therapeutic target to treat many metabolic disorders such as metabolic syndrome and some lysosomal storage disorders via targeting specific new signaling pathways, lipid molecules, and enzymes.
{"title":"Membrane lipids under norm and pathology","authors":"B. Marzoog, T. I. Vlasova","doi":"10.15584/EJCEM.2021.1.9","DOIUrl":"https://doi.org/10.15584/EJCEM.2021.1.9","url":null,"abstract":"Introduction. Lipid is an essential component of the cell and its organelles membrane. The uniqueness and selectivity of lipids to specific functions and asymmetry of lipid distribution in the organelle’s membrane give the cell ability of being highly qualified and specified. Aim. The paper provides a comprehensive review of membrane lipids in different tissues and organelles of the cell in norm and disease. Material and methods. The paper analyzed the present literature data on membrane lipids behavior in physiology and pathology. Analysis of the literature. The major structural and functional lipids of the cell membrane are phosphatidylcholine > phosphatidylethanolamine. The absence/deficiency or augmentation of a specific type of lipid results in serious defects and usually life-threatening with a permanent disability. The observations discussed here suggest, the lipid peroxidation severity depends on the membrane lipid composition of the cell. Some tissue cells can handle lipoperoxidation and protect themselves from the peroxidation damaging products better, while other cells cannot compensate. Therefore, some organs are highly sensitive to peroxidation and irreversible changes occur rapidly. Conclusion. To sum up, the understanding of lipid’s role in norm and disease is clinically crucial to evaluate a novel therapeutic target to treat many metabolic disorders such as metabolic syndrome and some lysosomal storage disorders via targeting specific new signaling pathways, lipid molecules, and enzymes.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73887783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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