Introduction. The concept of targeted drug delivery is nowadays based on nanoparticle transporters. Such drug delivery systems for cancer cells should follow the requirements like: efficient drug release, selective binding and internalization to cancer cells. The anticancer drug selectivity can be achieved by attachment of cancer cell-recognizing molecules, like biotin. Among nanosized carriers the PAMAM dendrimers are tested intensely, especially they can be modified by covalent attachment of prodrug molecules and biotin as targeting molecule. Aim. We aimed at construction and characterization of a conjugate formed between PAMAM and biotin (Biot). The nuclear magnetic resonances is powerful tool to determine both the structure and stoichiometry of the conjugate. Material and methods. PAMAM G0 has been synthesized and functionalized with biotin by reaction with N-hydroxysuccinimide ester of biotin to obtain G0 double-substituted with biotin. All the compound were thoroughly characterized by the NMR spectroscopy. Results. The conjugate of PAMAM G0 dendrimer with two amide-bonded biotin molecules was obtained and fully characterized by NMR spectroscopy. Conclusion. N-hydroxysuccinimide ester of biotin spontaneously reacts with PAMAM G0 to obtain the conjugate of 2:1 biotin: G0 stoichiometry. The latter was designed as a targeting molecule in formation of megameric multidrug delivery system.
{"title":"Low generation polyamidoamine dendrimers (PAMAM) and biotin-PAMAM conjugate – the detailed structural studies by 1H and 13C nuclear magnetic resonance spectroscopy","authors":"K. Wróbel, S. Wołowiec","doi":"10.15584/EJCEM.2020.4.4","DOIUrl":"https://doi.org/10.15584/EJCEM.2020.4.4","url":null,"abstract":"Introduction. The concept of targeted drug delivery is nowadays based on nanoparticle transporters. Such drug delivery systems for cancer cells should follow the requirements like: efficient drug release, selective binding and internalization to cancer cells. The anticancer drug selectivity can be achieved by attachment of cancer cell-recognizing molecules, like biotin. Among nanosized carriers the PAMAM dendrimers are tested intensely, especially they can be modified by covalent attachment of prodrug molecules and biotin as targeting molecule. Aim. We aimed at construction and characterization of a conjugate formed between PAMAM and biotin (Biot). The nuclear magnetic resonances is powerful tool to determine both the structure and stoichiometry of the conjugate. Material and methods. PAMAM G0 has been synthesized and functionalized with biotin by reaction with N-hydroxysuccinimide ester of biotin to obtain G0 double-substituted with biotin. All the compound were thoroughly characterized by the NMR spectroscopy. Results. The conjugate of PAMAM G0 dendrimer with two amide-bonded biotin molecules was obtained and fully characterized by NMR spectroscopy. Conclusion. N-hydroxysuccinimide ester of biotin spontaneously reacts with PAMAM G0 to obtain the conjugate of 2:1 biotin: G0 stoichiometry. The latter was designed as a targeting molecule in formation of megameric multidrug delivery system.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73725497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.15584/EJCEM.2020.4.10
Łukasz Gałuszka, Natalia Leksa, Dawid Leksa, A. Uryniak, D. Aebisher, D. Bartusik-Aebisher
Introduction. Sciatic vessels most often accompany the sciatic nerve. Sciatic vessels are very rare. Aim. In this paper we determine the procedure in sciatic vessels surgical treatment. Description of the case. We present the case of a 75-year-old patient with symptoms of acute right lower limb ischemia. The patient was discharged home in good condition, and remains in outpatient control to this day. Conclusion. The popliteal artery proved to be available, but much deeper than usual.
{"title":"Sciatic vessels – a case report","authors":"Łukasz Gałuszka, Natalia Leksa, Dawid Leksa, A. Uryniak, D. Aebisher, D. Bartusik-Aebisher","doi":"10.15584/EJCEM.2020.4.10","DOIUrl":"https://doi.org/10.15584/EJCEM.2020.4.10","url":null,"abstract":"Introduction. Sciatic vessels most often accompany the sciatic nerve. Sciatic vessels are very rare. Aim. In this paper we determine the procedure in sciatic vessels surgical treatment. Description of the case. We present the case of a 75-year-old patient with symptoms of acute right lower limb ischemia. The patient was discharged home in good condition, and remains in outpatient control to this day. Conclusion. The popliteal artery proved to be available, but much deeper than usual.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88943730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Truszkiewicz, D. Aebisher, Zuzanna Bober, Ł. Ożóg, D. Bartusik-Aebisher
Introduction. Magnetic Resonance Imaging (MRI) coils technology is a powerful improvement for clinical diagnostics. This includes opportunities for mathematical and physical research into coil design. Aim. Here we present the method applied to MRI coil array designs. Material and methods. Analysis of literature and self-research. Results. The coils that emit the radiofrequency pulses are designed similarly. As much as possible, they deliver the same strength of radiofrequency to all voxels within their imaging volume. Surface coils on the other hand are usually not embedded in cylindrical surfaces relatively close to the surface of the body. Conclusion. The presented here results relates to the art of magnetic resonance imaging (MRI) and RF coils design. It finds particular application of RF coils in conjunction with bore type MRI scanners.
{"title":"Radio Frequency MRI coils","authors":"A. Truszkiewicz, D. Aebisher, Zuzanna Bober, Ł. Ożóg, D. Bartusik-Aebisher","doi":"10.15584/ejcem.2020.1.5","DOIUrl":"https://doi.org/10.15584/ejcem.2020.1.5","url":null,"abstract":"Introduction. Magnetic Resonance Imaging (MRI) coils technology is a powerful improvement for clinical diagnostics. This includes opportunities for mathematical and physical research into coil design. Aim. Here we present the method applied to MRI coil array designs. Material and methods. Analysis of literature and self-research. Results. The coils that emit the radiofrequency pulses are designed similarly. As much as possible, they deliver the same strength of radiofrequency to all voxels within their imaging volume. Surface coils on the other hand are usually not embedded in cylindrical surfaces relatively close to the surface of the body. Conclusion. The presented here results relates to the art of magnetic resonance imaging (MRI) and RF coils design. It finds particular application of RF coils in conjunction with bore type MRI scanners.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80565760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction. The goals of health care provision include that it be accessible, acceptable, affordable and adequate. Discharge against medical advice (DAMA) is a failure of proper health care provision as there is disagreement arising from dissatisfaction with provided health care. DAMA is common in our sub-region because of many reasons; these includes ignorance, financial constraint of the patient, beliefs in unorthodox care and patients feeling that they are well when their caregivers do not think so. Aim. The objectives of this study are to determine the incidence, method of documentation of DAMA in the case notes and patients reasons for DAMA in our tertiary health institution. The A&E of any hospital in our environment attracts public criticism when there is dissatisfaction with services and DAMA when not handled well can lead to justifiable criticisms and/or litigations. Material and methods. This is a retrospective study. It was carried out at the adult accident and emergency department of Enugu state university of technology teaching hospital Enugu. Duration of the study was from January 2017 to December 2018. Results. A total of 8,152 patients were seen in the accident and emergency during this period. One hundred and seventy one (171) case notes were retrieved and reviewed for the study, DAMA rate of 2.1% was obtained. Fifty one folders (29.8%) did not have reason for the DAMA documented in them. The commonest reason for the DAMA was to seek traditional medical care with frequency of 17.5%. This was closely followed by financial constraint with 15.8%. Documentation for DAMA was done directly in the case notes. Conclusion. The incidence of DAMA from this study is similar to what is obtainable from other local studies, financial constraint on the patients and seeking alternative medical treatment were the commonest reasons for DAMA in our sub-region. Also, the documentation for the DAMA in this study was poorly done.
{"title":"Discharge against medical advice at the adult accident and emergency department in a tertiary hospital of a developing nation","authors":"H. Ekwedigwe, A. Edeh, A. Nevo, R. Ekwunife","doi":"10.15584/ejcem.2020.2.2","DOIUrl":"https://doi.org/10.15584/ejcem.2020.2.2","url":null,"abstract":"Introduction. The goals of health care provision include that it be accessible, acceptable, affordable and adequate. Discharge against medical advice (DAMA) is a failure of proper health care provision as there is disagreement arising from dissatisfaction with provided health care. DAMA is common in our sub-region because of many reasons; these includes ignorance, financial constraint of the patient, beliefs in unorthodox care and patients feeling that they are well when their caregivers do not think so. Aim. The objectives of this study are to determine the incidence, method of documentation of DAMA in the case notes and patients reasons for DAMA in our tertiary health institution. The A&E of any hospital in our environment attracts public criticism when there is dissatisfaction with services and DAMA when not handled well can lead to justifiable criticisms and/or litigations. Material and methods. This is a retrospective study. It was carried out at the adult accident and emergency department of Enugu state university of technology teaching hospital Enugu. Duration of the study was from January 2017 to December 2018. Results. A total of 8,152 patients were seen in the accident and emergency during this period. One hundred and seventy one (171) case notes were retrieved and reviewed for the study, DAMA rate of 2.1% was obtained. Fifty one folders (29.8%) did not have reason for the DAMA documented in them. The commonest reason for the DAMA was to seek traditional medical care with frequency of 17.5%. This was closely followed by financial constraint with 15.8%. Documentation for DAMA was done directly in the case notes. Conclusion. The incidence of DAMA from this study is similar to what is obtainable from other local studies, financial constraint on the patients and seeking alternative medical treatment were the commonest reasons for DAMA in our sub-region. Also, the documentation for the DAMA in this study was poorly done.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81831691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.15584/ejcem.2020.1.10
K. Akhtar, Saquib Alam, A. Rab, R. Sherwani
Introduction. Primary extrahepatic hydatid cysts are rare, and primary splenic hydatid cysts even rarer. Splenic hydatidosis constitutes 2% to 3.5% of all hydatid cysts. Aim. To present a case report of splenic hydatidosis with abdominal pain. Description of the case. We report here a case of isolated splenic hydatid cysts in a 23 year old female, who presented with dull dragging pain in the left hypochondrium. Diagnosis was made on computed tomography imaging of the abdomen and microscopic examination of the laminated hydatid cyst wall and supplemented with positive enzyme linked immunosorbent assay for hydatid antibodies. Conclusion. The incidence of splenic involvement by hydatid cysts is very low. Man is an accidental intermediate host, as entry of the larval forms into humans represents an end stage in its life cycle. Until recently the gold standard treatment for splenic hydatidosis was splenectomy, as medical therapy seems to be ineffective. However, the last two decades have shown a tendency towards splenic conservative surgery in suitable cases, to reduce opportunistic post splenectomy infection.
{"title":"Splenic hydatidosis with abdominal pain – a rare presentation in a developing nation","authors":"K. Akhtar, Saquib Alam, A. Rab, R. Sherwani","doi":"10.15584/ejcem.2020.1.10","DOIUrl":"https://doi.org/10.15584/ejcem.2020.1.10","url":null,"abstract":"Introduction. Primary extrahepatic hydatid cysts are rare, and primary splenic hydatid cysts even rarer. Splenic hydatidosis constitutes 2% to 3.5% of all hydatid cysts. Aim. To present a case report of splenic hydatidosis with abdominal pain. Description of the case. We report here a case of isolated splenic hydatid cysts in a 23 year old female, who presented with dull dragging pain in the left hypochondrium. Diagnosis was made on computed tomography imaging of the abdomen and microscopic examination of the laminated hydatid cyst wall and supplemented with positive enzyme linked immunosorbent assay for hydatid antibodies. Conclusion. The incidence of splenic involvement by hydatid cysts is very low. Man is an accidental intermediate host, as entry of the larval forms into humans represents an end stage in its life cycle. Until recently the gold standard treatment for splenic hydatidosis was splenectomy, as medical therapy seems to be ineffective. However, the last two decades have shown a tendency towards splenic conservative surgery in suitable cases, to reduce opportunistic post splenectomy infection.","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80486737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.15584/ejcem.2020.2.10
Dominika Przetacznik, Natalia Leksa, D. Aebisher, Sabina Galiniak, Seweryn Bartosz, Dawid Leksa, D. Bartusik-Aebisher
Introduction. The paper presents epidemiology, routes of infection, forms of the disease, diagnostic and treatment methods, and prophylaxis of tick-borne encephalitis. Aim. In this paper, we present two descriptions of the cases of tick-borne encephalitis. Description of the cases. Case 1. A 60-year-old man with fever up to 39 degrees for 3 days, multi-site headache and other body aches, as well as an earstuck feeling. The day before hospitalization, there was a feeling of numbness on the right side of the face from eye level to the chin and speech distortion. Case 2. A 60-year-old patient with headaches and an increase in temperature to 39 degrees for 3 days, who, approximately, three weeks earlier was ticked by a tick in the lower parts of the back. Conclusion. It is also important for doctors to take a broader view and to make society aware of that Lyme disease is not associated only with Lyme disease. Further work is also needed towards effective treatments for Tick-borne encephalitis (TBE).
{"title":"Diagnostic and therapeutic difficulties of tick-borne encephalitis – a two case reports","authors":"Dominika Przetacznik, Natalia Leksa, D. Aebisher, Sabina Galiniak, Seweryn Bartosz, Dawid Leksa, D. Bartusik-Aebisher","doi":"10.15584/ejcem.2020.2.10","DOIUrl":"https://doi.org/10.15584/ejcem.2020.2.10","url":null,"abstract":"Introduction. The paper presents epidemiology, routes of infection, forms of the disease, diagnostic and treatment methods, and prophylaxis of tick-borne encephalitis. Aim. In this paper, we present two descriptions of the cases of tick-borne encephalitis. Description of the cases. Case 1. A 60-year-old man with fever up to 39 degrees for 3 days, multi-site headache and other body aches, as well as an earstuck feeling. The day before hospitalization, there was a feeling of numbness on the right side of the face from eye level to the chin and speech distortion. Case 2. A 60-year-old patient with headaches and an increase in temperature to 39 degrees for 3 days, who, approximately, three weeks earlier was ticked by a tick in the lower parts of the back. Conclusion. It is also important for doctors to take a broader view and to make society aware of that Lyme disease is not associated only with Lyme disease. Further work is also needed towards effective treatments for Tick-borne encephalitis (TBE).","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86627208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.15584/ejcem.2019.4.16
Rafal M. Dziedzic, Natalia Leksa, D. Aebisher, D. Bartusik-Aebisher
{"title":"Granular cell tumor of the neurohypophysis – a case report","authors":"Rafal M. Dziedzic, Natalia Leksa, D. Aebisher, D. Bartusik-Aebisher","doi":"10.15584/ejcem.2019.4.16","DOIUrl":"https://doi.org/10.15584/ejcem.2019.4.16","url":null,"abstract":"","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75985233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Romanova, O. Zolotukhina, S. Shnaider, L. Kravchenko, N. Noneva
{"title":"A new local therapy of periodontitis in the course of stomach pathology and tobacco smoke intoxication","authors":"I. Romanova, O. Zolotukhina, S. Shnaider, L. Kravchenko, N. Noneva","doi":"10.15584/ejcem.2019.4.3","DOIUrl":"https://doi.org/10.15584/ejcem.2019.4.3","url":null,"abstract":"","PeriodicalId":15378,"journal":{"name":"临床和实验医学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91207408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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