Journal of Dental Sciences最新文献_第5页

Potential effect of topical antibiotics administration in the oral cavity on the reduced number of bacteria entering the lower respiratory tract after oral cancer surgery 口腔局部使用抗生素对减少口腔癌手术后进入下呼吸道的细菌数量的潜在影响

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.06.021

Akira Imakiire, S. Soutome, Kei-ichiro Miura, Nanako Ito, M. Higaki, F. Obayashi, Koichi Koizumi, Souichi Yanamoto, Tomohiro Yamada, Masahiro Umeda

引用次数: 0

The expression of immune co-stimulators as a prognostic predictor of head and neck squamous cell carcinomas and oral squamous cell carcinomas 免疫共刺激因子的表达作为头颈部鳞状细胞癌和口腔鳞状细胞癌的预后预测指标

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.05.004

Shi-Rou Chang , Chung-Hsien Chou , Hsi-Feng Tu , Chung-Ji Liu , Kuo-Wei Chang , Shu-Chun Lin

Background/purpose

T cells require second immune checkpoint molecules for activation and immune memory after antigen presentation. We found that inducible co-stimulator (ICOS) has been a favorable prognostic factor amongst B7 immune checkpoint co-stimulators (ICSs) families in head and neck squamous cell carcinoma (HNSCC) and oral SCC (OSCC).

Materials and methods

This study analyzed the expression of non-B7 tumor necrosis factor (TNF) superfamily ICSs in the Cancer Genome Atlas (TCGA) HNSCC cohort, our OSCC cohort, and TCGA pan-cancer datasets. The correlation in expression, prognosis, and immune status was assessed.

Results

The higher expression of CD27, CD30, CD40L, death domain 3 (DR3), and OX40, presumably on the T cell surface, defined better overall survival of HNSCC patients. Besides, CD27, CD30, CD40L, and OX40 were highly correlated with ICOS expression in tumors. CD27, CD40L, and DR3 expression are higher in HPV+ HNSCC tumors than in HPV- tumors. The combined expression level of CD27/OX40 or CD27/CD40L/OX40 enables the potent survival prediction of small, less nodal involvement, early stage, and HPV + tumor subsets. Tumors expressing high CD27, CD30, CD40L, ICOS, and OX40 exhibited enhanced immune cell infiltration. The high correlation in the expression of these ICSs was also noted in the vast majority of tumor types in TCGA datasets.

Conclusion

The findings of this study not only confirm the potential of the concordant stimulation of CD27, CD30, CD40L, ICOS, and OX40 as a crucial strategy in cancer immunotherapy but also inspire further exploration into the field, highlighting the promising future of cancer treatment.

背景/目的T细胞在抗原呈递后需要第二个免疫检查点分子来激活和免疫记忆。我们发现，在头颈部鳞状细胞癌（HNSCC）和口腔 SCC（OSCC）的 B7 免疫检查点辅助刺激因子（ICSs）家族中，诱导性辅助刺激因子（ICOS）是一个有利的预后因素。材料与方法本研究分析了癌症基因组图谱（TCGA）HNSCC队列、我们的OSCC队列和TCGA泛癌症数据集中非B7肿瘤坏死因子（TNF）超家族ICSs的表达。结果CD27、CD30、CD40L、死亡结构域3（DR3）和OX40（可能在T细胞表面）的表达量越高，HNSCC患者的总生存率越高。此外，CD27、CD30、CD40L和OX40与肿瘤中ICOS的表达高度相关。CD27、CD40L和DR3在HPV+ HNSCC肿瘤中的表达高于HPV-肿瘤。CD27/OX40或CD27/CD40L/OX40的联合表达水平能有效预测小肿瘤、少结节累及肿瘤、早期肿瘤和HPV+肿瘤亚群的生存率。高表达 CD27、CD30、CD40L、ICOS 和 OX40 的肿瘤表现出更强的免疫细胞浸润。结论本研究的发现不仅证实了 CD27、CD30、CD40L、ICOS 和 OX40 的协同刺激作为癌症免疫疗法关键策略的潜力，而且还激发了对该领域的进一步探索，彰显了癌症治疗的美好前景。

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引用次数: 0

Course content analysis of the last dental course for the medical students of National Taiwan University in 2011 2011 年台湾大学医科学生最后一门牙科课程的课程内容分析

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.05.023

Feng-Chou Cheng, Ling-Hsia Wang, Yin-Lin Wang, Chun-Pin Chiang

引用次数: 0

Effect of oral function and postoperative eating patterns on salivary bacterial counts in gastrointestinal tract surgery patients: A preliminary study 口腔功能和术后饮食方式对胃肠道手术患者唾液细菌计数影响的初步研究

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2023.11.007

Yuki Sakamoto , Makiko Moriyama , Arisa Tanabe , Madoka Funahara , Sakiko Soutome , Akira Imakiire , Masahiro Umeda , Yuka Kojima

Background/purpose

Perioperative oral care is widely provided to prevent postoperative pneumonia and surgical site infections in patients undergoing surgery under general anesthesia. However, there is a lack of clarity regarding the kind of oral care that should be provided for different patients. The purpose of this study was to clarify the factors that influence the increase in salivary bacterial counts before and after gastrointestinal surgery to identify patients with a particular need for oral care.

Materials and methods

Twenty patients undergoing gastrointestinal surgery were examined before surgery for denture use, number of remaining teeth, regular dental care, Oral Hygiene Index-Simplfied tongue coating, tongue pressure, bite pressure, masticatory efficiency, and dry mouth. Saliva samples were collected before surgery, in the fasting period after surgery, and in the oral feeding period. Total bacterial counts were determined by real-time PCR, and factors associated with bacterial counts were investigated.

Results

Patients with decreased oral functions, such as tongue pressure, bite pressure, and masticatory efficiency, tended to have higher salivary bacterial counts in the preoperative, fasting, and oral feeding periods. Regarding the pre- and postoperative changes, salivary bacterial counts increased in the fasting period compared to the pre-operative period and returned to preoperative values in the oral feeding period.

Conclusion

Perioperative oral care is important for patients with reduced oral function because the number of bacteria in saliva tends to be higher in such patients. As the number of bacteria in saliva increases during the fasting period, oral care is important, and oral feeding should be restarted as soon as possible.

背景/目的围手术期口腔护理被广泛用于预防全麻手术患者术后肺炎和手术部位感染。然而，对于应该为不同的患者提供何种口腔护理，目前尚不清楚。本研究的目的是澄清影响胃肠道手术前后唾液细菌计数增加的因素，以确定有特殊口腔护理需求的患者。材料与方法对20例胃肠手术患者术前进行义齿使用情况、剩余牙数、常规口腔保健、口腔卫生指数-简化舌苔、舌压、咬压、咀嚼效率、口干等检查。分别于术前、术后禁食期及口服喂养期采集唾液样本。采用实时荧光定量PCR法测定细菌总数，并分析影响细菌总数的因素。结果舌压、咬压、咀嚼效率等口腔功能下降的患者在术前、禁食和口服喂养期唾液细菌计数较高。在术前和术后变化方面，禁食期唾液细菌计数较术前增加，而口服喂养期唾液细菌计数恢复到术前水平。结论口腔功能减退患者唾液中细菌数量较多，围手术期口腔护理十分重要。由于禁食期间唾液中的细菌数量增加，口腔护理很重要，应尽快重新开始口服喂养。

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引用次数: 0

The histone deacetylase inhibitor MS-275 enhances the matrix mineralization of dental pulp stem cells by inducing fibronectin expression 组蛋白去乙酰化酶抑制剂MS-275通过诱导纤维连接蛋白表达增强牙髓干细胞基质矿化

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2023.11.019

Shigeki Suzuki, Kento Sasaki, Rahmad Rifqi Fahreza, Eiji Nemoto, Satoru Yamada

Background/purpose

The acetylation of histone H3 proteins keeps local chromatin regions open and accessible, thereby facilitating transcriptional events. We recently reported integrative epigenomic and transcriptome analyses of differentiating dental pulp stem cells (DPSCs). A significant increase in the number of super-enhancers, which are local genomic locations marked by condensed open chromatin peaks that facilitate transcriptional events, in differentiating DPSCs were observed. However, it is still unclear whether histone deacetylase (HDACs) inhibitors (HDACis) have beneficial effects on the odontogenic differentiation of DPSCs and on the matrix mineralization-inducing ability of DPSCs.

Materials and methods

DPSCs were cultured in an odontogenic induction medium for a prolonged period in the presence of HDACis, MS-275 and Trichostatin A (TSA). ATAC-seq and RNA-seq samples were collected from differentiating DPSCs to explore the epigenomic and transcriptomic alterations induced by HDACis and identify key target proteins that mediate HDACis-induced phenotypic changes.

Results

MS-275 and TSA did not change whole-genome open chromatin accessibility or increase odontogenic differentiation, as assessed by alkaline phosphate activity. However, the matrix mineralization-inducing ability assessed by calcified nodule formation was significantly increased by MS-275 but not by TSA. FN1, which encodes fibronectin, was identified as upregulated by MS-275. The knockdown of fibronectin evidently suppressed MS-275-induced calcified nodule formation.

Conclusion

MS-275 induced calcified nodule formation by the mechanistic upregulation of FN1, independent of epigenomic alterations. Hence, the application of MS-275 as direct capping materials has therapeutic potential for promoting reparative dentin formation by constructing a fibronectin-organizing physiological extracellular matrix environment that is adequate for matrix mineralization.

背景/目的组蛋白H3的乙酰化使局部染色质区域保持开放和可达性，从而促进转录事件。我们最近报道了分化牙髓干细胞(DPSCs)的综合表观基因组和转录组分析。在分化的DPSCs中，观察到超增强子的数量显著增加，超增强子是由浓缩开放染色质峰标记的局部基因组位置，促进转录事件。然而，组蛋白去乙酰化酶(HDACs)抑制剂(HDACis)是否对DPSCs的成牙性分化和DPSCs的基质矿化诱导能力有有益影响尚不清楚。材料和方法sdpscs在HDACis、MS-275和Trichostatin a (TSA)存在下的成牙诱导培养基中长时间培养。从分化的DPSCs中收集ATAC-seq和RNA-seq样本，探索HDACis诱导的表观基因组和转录组改变，并鉴定介导HDACis诱导的表型变化的关键靶蛋白。结果sm -275和TSA没有改变全基因组开放染色质可及性或增加牙源性分化，通过碱性磷酸盐活性评估。然而，通过钙化结节形成来评估基质矿化诱导能力，MS-275显著增加，而TSA没有。编码纤维连接蛋白的FN1被MS-275上调。纤维连接蛋白的下调明显抑制ms -275诱导的钙化结节形成。结论ms -275通过上调FN1蛋白的机制诱导钙化结节形成，不依赖于表观基因组的改变。因此，MS-275作为直接封盖材料的应用具有促进修复性牙本质形成的治疗潜力，它通过构建一个纤维连接蛋白组织的生理细胞外基质环境来促进基质矿化。

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引用次数: 0

Surface strain at the cervical area and fracture strength of flared root canals reinforced using a zirconia tube and glass-fiber post 使用氧化锆管和玻璃纤维桩加固的扩口根管颈部表面应变和折断强度

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2023.12.012

Daiki Kondo, Wataru Komada, Shinya Oishi, Kenji Fueki

Background/purpose

Recently, an effective core build-up system for teeth with flared root canals is needed. This research aimed to evaluate the effect of foundation restorations using a composite resin core with a fiber post reinforced with a zirconia tube for the surface strain at the cervical area and the fracture load of teeth with flared root canals.

Materials and methods

Bovine teeth were shaped to mimic human premolars with flared root canals and restored using three types of composite resin foundation restorations with each materials described below: a fiber post (FC), a zirconia tube (ZC), a fiber post and zirconia tube (ZFC). Each specimen was restored with a zirconia crown. The surface strains of the specimens at the cervical area and fracture loads were analyzed using a one-way analysis of variance (ANOVA), followed by Tukey's honest significant difference test.

Results

The surface strains of Groups ZFC and ZC were significantly lower than that of Group FC in the buccal root. The fracture strengths of Groups ZFC and ZC were significantly higher than that of Group FC. The strength of Group ZFC was significantly higher than that of Group ZC.

Conclusion

The use of a composite resin core with a zirconia tube for the simulated premolar with flared root canals reduced surface strain at the cervical area and provided higher fracture strength compared to using a composite resin core with a fiber post. And the zirconia tubes provided even higher fracture strength when used with a fiber post.

背景/目的近来，需要一种有效的根管外翻牙齿的核心建立系统。本研究旨在评估使用带有纤维桩和氧化锆管增强的复合树脂核心的基础修复体对根管外翻的牙齿的颈部表面应变和折断负荷的影响。材料和方法模拟带有根管外翻的人类前磨牙，使用三种类型的复合树脂基础修复体进行修复，每种材料如下所述：纤维桩（FC）、氧化锆管（ZC）、纤维桩和氧化锆管（ZFC）。每个试样都用氧化锆冠修复。采用单因素方差分析（ANOVA）对试样颈部的表面应变和断裂载荷进行分析，然后进行 Tukey's 诚实显著性差异检验。结果 ZFC 和 ZC 组在颊根部的表面应变明显低于 FC 组。ZFC 和 ZC 组的断裂强度明显高于 FC 组。结论与使用带有纤维桩的复合树脂牙核相比，使用带有氧化锆管的复合树脂牙核来治疗根管外翻的模拟前磨牙可以减少牙颈部的表面应变，并提供更高的折断强度。而氧化锆管与纤维桩一起使用时，断裂强度更高。

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引用次数: 0

In vitro evaluation of the impact of a bioceramic root canal sealer on the mechanical properties of tooth roots 体外评估生物陶瓷根管封闭剂对牙根机械性能的影响

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2023.10.013

Ya-Hui Wang, Si-Yi Liu, Yan-Mei Dong

Bacground/purpose

Endodontically treated teeth are more prone to vertical root fracture with the mechanical property changes to some extent during root canal treatment. This study aimed to investigate the effects of a bioceramic sealer on the mechanical properties of tooth roots.

Materials and methods

Dentin discs were dried by two different methods (ethanol drying and paper points drying) and then filled with a BC sealer named iRoot SP. SEM and EDS were used to analyze the newly formed minerals in dentin tubules. Elastic modulus and hardness of the secondary dentin in areas proximal to the primary dentin (PD-SD) and areas proximal to canal or iRoot SP (SD-C/SD-iRoot SP) were measured using nanoindentation technique. The compressive strength of roots filled with iRoot SP were tested by compressive loading test.

Results

(1) Penetration and mineralization: Paper points drying was more conducive to iRoot SP adhesion, spreading and penetration into the dentin tubules than 95% ethanol drying. (2) Micromechanical properties: After filling root canal with iRoot SP, the elastic modulus and hardness of SD-iRoot SP were higher than those of PD-SD (P = 0.001 and P = 0.000). (3) Fracture resistance: The compressive strength of the roots filled with iRoot SP was not significantly different from that of the roots unprepared and unfilled (P = 0.957), but was higher than that of the roots prepared and unfilled (P = 0.009).

Conclusion

Excessive drying (95% ethanol drying method) is not conducive to the penetration and mineralization of the BC sealer iRoot SP into dentin tubules. The good bioactivity of iRoot SP was responsible for increasing the elastic modulus and hardness of dentin, which strengthened the prepared roots.

背景/目的根管治疗的牙齿更容易发生垂直根折，在根管治疗过程中牙根的机械性能会发生一定程度的变化。本研究旨在探讨生物陶瓷封闭剂对牙根机械性能的影响。材料和方法用两种不同的方法（乙醇烘干和纸点烘干）烘干牙本质圆片，然后填充一种名为 iRoot SP 的生物陶瓷封闭剂。使用 SEM 和 EDS 分析牙本质小管中新形成的矿物质。使用纳米压痕技术测量了主牙本质近端区域（PD-SD）和根管或 iRoot SP 近端区域（SD-C/SD-iRoot SP）次生牙本质的弹性模量和硬度。结果（1）渗透和矿化：纸点干燥比 95% 乙醇干燥更有利于 iRoot SP 黏附、扩散和渗透到牙本质小管中。(2）微机械性能：用 iRoot SP 填充根管后，SD-iRoot SP 的弹性模量和硬度均高于 PD-SD （P = 0.001 和 P = 0.000）。(3) 抗折性：结论过度干燥（95% 乙醇干燥法）不利于 BC 封闭剂 iRoot SP 在牙本质小管中的渗透和矿化。iRoot SP 良好的生物活性提高了牙本质的弹性模量和硬度，从而强化了制备好的牙根。

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引用次数: 0

Ethnicity, gender, and geographical distribution of dentists in Taiwan in 1939 (Showa 14) 1939 年台湾牙医的种族、性别和地理分布（昭和 14 年）

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.03.020

Feng-Chou Cheng , Ling-Hsia Wang , Tzu-Chiang Lin , Julia Yu-Fong Chang , Chun-Pin Chiang

Background/purpose

Taiwan's dentistry entered into a new era of modernization and flourished during the Japanese colonial period. However, we know very little about the composition of dentists at that time. This study attempted to analyze the ethnicity, gender, and geographical distribution of dentists in Taiwan in 1939 (Showa 14).

Materials and methods

The methods of documentary analysis and secondary data analysis were adopted to find the composition of dentists during the late Japanese colonial period through a name list of contract dentists for the Postal Savings Insurance published in August 1939 (Showa 14) by the Taiwan Government Transportation Department Information Bureau.

Results

The total number of contract dentists was 368, accounting for 86.79% of the 424 practicing dentists in Taiwan in 1939 (Showa 14). Of the 368 contract dentists (328 males and 40 females), 225 (61.14%) were Taiwanese and 143 (38.86%) were Japanese. Among the 8 prefectures in Taiwan, Tainan Prefecture had the largest number of dentists (97), followed by Taipei (84) and Taichung (78) prefectures. The number of contract dentists per 100,000 people was 6.24, equivalent to 16,021 people served by each contract dentist in 1939 (Showa 14). The chi-square test for the trend analysis of gender distribution indicated a significantly higher proportion of male than female contract dentists in either Taiwanese or Japanese ethnic group.

Conclusion

In the late Japanese colonial period, the number of Taiwanese dentists exceeded that of Japanese dentists. Furthermore, there were more male than female dentists in either the Taiwanese or the Japanese population.

背景/目的在日本殖民时期，台湾的牙科进入了现代化的新时代，并蓬勃发展。然而，我们对当时牙科医生的构成知之甚少。本研究试图分析 1939 年（昭和 14 年）台湾牙医的种族、性别和地理分布。材料和方法采用文献分析和二手数据分析的方法，通过台湾政府交通部新闻局 1939 年 8 月（昭和 14 年）公布的邮政储蓄保险签约牙医名单，了解日本殖民地后期台湾牙医的构成。在 368 名合同牙医中（男性 328 人，女性 40 人），台湾人 225 人（占 61.14%），日本人 143 人（占 38.86%）。在台湾的 8 个县中，台南县的牙医人数最多（97 人），其次是台北县（84 人）和台中县（78 人）。每 10 万人中的签约牙医人数为 6.24 人，相当于 1939 年（昭和 14 年）每名签约牙医服务了 16 021 人。性别分布趋势分析的卡方检验表明，无论是台湾族群还是日本族群，男性签约牙医的比例都明显高于女性。此外，无论是台湾人还是日本人，男性牙医的人数都多于女性。

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引用次数: 0

Increased expression of keratin 17 in oral lichen planus and its correlation with disease severity 口腔扁平苔藓中角蛋白 17 的表达增加及其与疾病严重程度的关系

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.01.016

Xinyi Han , Ran Zhao , Qianqian Zhang, Xuemin Shen, Kai Sun

Background/purpose

Oral lichen planus (OLP) is a chronic inflammatory disease with unknown mechanisms of pathogenesis. Keratin 17 (KRT17) is a protein that regulates numerous cellular processes. This study aimed to explore the expression of KRT17 in OLP and its correlation with the severity of OLP.

Materials and methods

RNA sequencing using epithelium from 5 OLP patients and 5 health control (HC) was performed, followed by functional analysis. The validation cohort of 20 OLP and 20 HC tissues were used to investigate positive area value of KRT17 by immunohistochemical analysis. Reticular, erosive and ulcerative (REU) scores were used for measuring the severity of OLP.

Results

A total of 15493 genes were detected, of which 1492 genes were significantly up-regulated in OLP and 622 were down-regulated. The mRNA expression of KRT17 was elevated by 13.09-fold in OLP compared to that in HC. Pathway analysis demonstrated high KRT17 expression was associated with multiple biological processes. The median of percentage of KRT17 positive area value was 19.30 % in OLP and 0.01 % in HC (P < 0.001). Percentage of KRT17 positive area value was higher in erosive OLP patients (27.25 %) compared to that in non-erosive patients (15.02 %, P = 0.006). REU scores were positively correlated with percentage of KRT17 positive area value (r = 0.628, P = 0.003).

Conclusion

The mRNA expression of KRT17 was elevated in OLP tissues compared to that in HC. KRT17 was positively correlated with the severity of OLP, indicating KRT17 might play a vital role in the pathogenesis of OLP.

背景/目的口腔扁平苔藓（OLP）是一种慢性炎症性疾病，发病机制不明。角蛋白17（KRT17）是一种调节多种细胞过程的蛋白质。本研究旨在探讨KRT17在OLP中的表达及其与OLP严重程度的相关性。材料与方法利用5名OLP患者和5名健康对照者（HC）的上皮细胞进行RNA测序，然后进行功能分析。对 20 例 OLP 和 20 例 HC 组织进行验证，通过免疫组化分析研究 KRT17 的阳性面积值。结果共检测到 15493 个基因，其中 1492 个基因在 OLP 中显著上调，622 个基因下调。与HC相比，KRT17的mRNA表达在OLP中升高了13.09倍。通路分析表明，KRT17的高表达与多个生物学过程相关。KRT17阳性面积百分比的中位数在OLP中为19.30%，在HC中为0.01%（P < 0.001）。侵蚀性OLP患者的KRT17阳性面积值百分比（27.25%）高于非侵蚀性患者（15.02%，P = 0.006）。REU评分与KRT17阳性面积百分比呈正相关（r = 0.628, P = 0.003）。KRT17与OLP的严重程度呈正相关，表明KRT17可能在OLP的发病机制中发挥重要作用。

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引用次数: 0

Managing the second mesiobuccal canal of maxillary first molars 上颌第一磨牙第二中颊面管的管理

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2024-07-01 DOI: 10.1016/j.jds.2024.05.011

Kaveh Nasiri, Karl-Thomas Wrbas, Maryam Kuzekanani

引用次数: 0