Journal of Dental Sciences最新文献_第5页

Metformin alleviates the progression of oral submucous fibrosis through downregulation of metastasis associated lung adenocarcinoma transcript 1 (MALAT1) 二甲双胍通过下调转移相关肺腺癌转录物1 （MALAT1）缓解口腔黏膜下纤维化的进展

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.07.001

Pei-Ling Hsieh , Fang-Yu Chang , Po-Hsuan Tang , Yi-Wen Liao , Cheng-Chia Yu , Chia-Ming Liu

Background

/purpose: Emerging evidence demonstrates the anti-fibrotic properties of metformin, a first-line anti-diabetic drug; however, its inhibitory effects on oral fibrogenesis warrant further evaluation.

Materials and methods

The cytotoxic effects of metformin on normal and fibrotic buccal mucosal fibroblasts (fBMFs) derived from OSF tissues were evaluated using the MTT assay. Collagen gel contraction, wound healing, and transwell migration assays were carried out to assess myofibroblast features. In addition, the expression levels of alpha-1 type I collagen (COL1A1), alpha-smooth muscle actin (α-SMA) and Smad2 were measured. Moreover, RNA sequencing was performed to explore potential targets participating in the anti-fibrotic effects of metformin.

Results

A lower dose of metformin was sufficient to inhibit the proliferation of fBMFs without affecting normal BMFs, and to attenuate various myofibroblast characteristics, including collagen gel contraction, wound healing, transwell migration capacities, and the expression of fibrosis markers. Additionally, we showed that administration of metformin prevented the arecoline-induced myofibroblast activation. Most importantly, our results suggest that metformin may exert suppressive effects on myofibroblast activities by inhibiting ROS accumulation through the downregulation of MALAT1.

Conclusion

These findings indicate that metformin may serve as a preventive agent against OSF progression.

背景/目的：新出现的证据证明了一线降糖药二甲双胍的抗纤维化特性；然而，其对口腔纤维生成的抑制作用有待进一步评估。材料和方法采用MTT法观察二甲双胍对口腔粘膜正常及纤维化成纤维细胞的细胞毒作用。胶原凝胶收缩、伤口愈合和跨井迁移试验用于评估肌成纤维细胞的特征。同时检测α- 1型胶原蛋白（COL1A1）、α-平滑肌肌动蛋白（α-SMA）、Smad2的表达水平。此外，进行RNA测序以探索参与二甲双胍抗纤维化作用的潜在靶点。结果低剂量二甲双胍足以抑制fBMFs的增殖，而不影响正常BMFs，并减弱肌成纤维细胞的各种特征，包括胶原凝胶收缩、伤口愈合、跨井迁移能力和纤维化标志物的表达。此外，我们发现二甲双胍可以阻止槟榔碱诱导的肌成纤维细胞活化。最重要的是，我们的研究结果表明，二甲双胍可能通过下调MALAT1抑制ROS积累，从而对肌成纤维细胞活性产生抑制作用。结论二甲双胍可作为预防OSF进展的药物。

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引用次数: 0

Ferritin promotes monocyte osteoclastogenic differentiation to aggravate alveolar bone resorption in periodontitis 铁蛋白促进单核细胞破骨分化，加重牙周炎患者牙槽骨吸收

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.05.034

Wenxue Huang , Jie Zhang , Menglong Hu , Zhaoguo Yue , Ye Han , Xiaochi Chang , Jianxia Hou

Background/purpose

Alveolar bone resorption in periodontitis critically impairs masticatory function and tooth stability, yet the mechanistic drivers linking immune dysregulation to osteolytic pathology remain incompletely characterized. This study elucidates how ferritin-an inflammation responsive iron chaperone secreted by activated monocytes-orchestrates monocyte-to-osteoclast differentiation to exacerbate bone destruction in periodontitis.

Materials and methods

Using a multidisciplinary approach, we combined: Immunohistochemical profiling of cluster of differentiation 68-positive (CD68⁺) cells and cluster of differentiation 4-positive (CD4⁺) cells in human periodontal tissues (periodontitis vs. healthy controls); Murine experimental periodontitis induced by maxillary molar ligation (8-week model); In vitro mechanistic studies where THP-1 monocytes (human acute monocytic leukemia cell line) primed with Porphyromonas gingivalis lipopolysaccharide (P. gingivalis-LPS) were analyzed for ferritin/receptor activator of nuclear factor-κB ligand (RANKL) secretion, and RAW264.7 pre-osteoclasts (murine macrophage-like cell line) were treated with apoferritin ± RANKL to assess proliferation, osteoclast differentiation, and osteolytic gene expression.

Results

Inflamed periodontal tissues exhibited elevated CD68⁺ and CD4⁺ immune cells, reflecting heightened inflammatory activity. P. gingivalis-LPS stimulation increased ferritin and RANKL secretion in THP-1 cells. Mice with experimental periodontitis showed increased osteoclast density at alveolar bone surfaces. Apoferritin synergized with RANKL to enhance RAW264.7 proliferation, multinucleation, and expression of matrix metallopeptidase 9 (MMP-9), cathepsin K (CTSK), and tartrate-resistant acid phosphatase (TRAP) in a dose-dependent manner.

Conclusion

Ferritin and RANKL synergistically promote monocyte osteoclastogenesis, driving alveolar bone resorption in periodontitis. Targeting ferritin signaling may offer therapeutic potential to mitigate bone loss.

背景/目的牙周炎患者的牙槽骨吸收严重损害咀嚼功能和牙齿稳定性，但免疫失调与溶骨病理之间的机制驱动因素尚未完全确定。本研究阐明了铁蛋白（一种由活化单核细胞分泌的炎症反应性铁伴侣）如何协调单核细胞向破骨细胞的分化，从而加剧牙周炎的骨破坏。材料和方法采用多学科方法，我们结合了：人类牙周组织（牙周炎与健康对照）中分化68阳性（CD68+）细胞簇和分化4阳性（CD4+）细胞簇的免疫组织化学分析；上颌磨牙结扎致小鼠实验性牙周炎（8周模型）在体外机制研究中，分析了以牙龈卟啉单胞菌脂多糖（P. gingivalis- lps）引发的THP-1单核细胞（人急性单核白血病细胞系）分泌铁蛋白/核因子-κB配体受体激活剂（RANKL），并以载铁蛋白±RANKL处理RAW264.7前破骨细胞（小鼠巨噬细胞样细胞系），以评估其增殖、破骨细胞分化和溶骨基因表达。结果牙周组织中CD68+和CD4+免疫细胞升高，炎症活性增强。P. gingivalis-LPS刺激增加THP-1细胞铁蛋白和RANKL分泌。实验性牙周炎小鼠牙槽骨表面破骨细胞密度增加。载铁蛋白与RANKL协同作用，以剂量依赖性方式增强RAW264.7增殖、多核以及基质金属肽酶9 （MMP-9）、组织蛋白酶K （CTSK）和酒石酸盐抗性酸性磷酸酶（TRAP）的表达。结论铁蛋白与RANKL协同促进单核细胞破骨生成，促进牙周炎患者牙槽骨吸收。针对铁蛋白信号可能提供治疗潜力，以减轻骨质流失。

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引用次数: 0

Evaluating thymol vapor for biofilm removal and biocompatibility in curved root canal models in vitro 评价百里香酚蒸气对体外弯曲根管模型生物膜去除及生物相容性

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.09.001

Maria Paulene Dee Manuel , Yin-Hwa Shih , Shih-Min Hsia , Tong-Hong Wang , Yu-Hsin Tseng , Ming-Gene Tu , Tzong-Ming Shieh

Background/purpose

Conventional irrigants such as chlorhexidine and sodium hypochlorite have strong antimicrobial properties but high cytotoxicity, limiting their use in regenerative endodontics. We hypothesized that thymol vapor could provide effective antibacterial activity with lower cytotoxicity.

Materials and methods

The antimicrobial activity of thymol in both liquid and vapor phases was tested against Enterococcus faecalis, Streptococcus mutans, and Aggregatibacter actinomycetemcomitans using a resin block model simulating curved root canals. The effect of thymol vapor, alone or with mechanical instrumentation, was tested on early-stage biofilms removal. Cytotoxicity was assessed using MTT assays in L-929 fibroblasts and MG-63 osteoblast-like cells, and pro-inflammatory cytokine gene expression (IL-1β, TNF-α, IL-6) was measured via qRT-PCR.

Results

Thymol exhibited minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.8–1.0 mg/mL in planktonic cultures. In early-stage biofilms models, 10–100 mg/mL liquid thymol and 5.0 mg/mL thymol vapor significantly reduced bacterial viability. Combining 1.0 mg/mL thymol vapor with mechanical instrumentation enhanced early-stage biofilms removal, particularly against E. faecalis. Direct exposure to thymol and chlorhexidine caused significant cytotoxicity, while 1.0 mg/mL vapor showed lower cytotoxic and did not significantly induce pro-inflammatory cytokine genes in L-929 cells. At higher concentrations, MG-63 cells exhibited increased cytokine expression.

Conclusion

This study is the first to propose thymol vapor for biofilm removal in curved root canal models and to demonstrate its antibacterial activity with lower cytotoxicity than conventional irrigants. Its potential as an adjunct in regenerative endodontics merits further investigation, particularly in relation to immunomodulatory effects.

背景/目的氯己定和次氯酸钠等常规冲洗剂具有很强的抗菌性能，但细胞毒性高，限制了它们在再生牙髓学中的应用。我们假设百里香酚蒸气具有较低的细胞毒性和有效的抗菌活性。材料与方法采用模拟弯曲根管的树脂块模型，测定百里香酚在液相和气相对粪肠球菌、变形链球菌和放线菌聚集菌的抑菌活性。研究了百里香酚蒸汽单独或配合机械仪器对早期生物膜去除的影响。采用MTT法检测L-929成纤维细胞和MG-63成骨细胞样细胞的细胞毒性，采用qRT-PCR法检测促炎细胞因子（IL-1β、TNF-α、IL-6）的基因表达。结果百里香酚对浮游生物的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）为0.8 ~ 1.0 mg/mL。在早期生物膜模型中，10-100 mg/mL液体百里香酚和5.0 mg/mL百里香酚蒸气显著降低细菌活力。1.0 mg/mL百里酚蒸汽与机械仪器结合可增强早期生物膜的去除，特别是对粪肠球菌的去除。直接暴露于百里香酚和氯己定对L-929细胞具有显著的细胞毒性，而1.0 mg/mL蒸气对L-929细胞的细胞毒性较低，且对促炎细胞因子基因的诱导作用不显著。在较高浓度下，MG-63细胞的细胞因子表达增加。结论本研究首次提出了百里香酚蒸汽用于弯曲根管模型生物膜的去除，并证明其抗菌活性低于常规冲洗剂的细胞毒性。它作为再生牙髓学辅助材料的潜力值得进一步研究，特别是在免疫调节作用方面。

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引用次数: 0

Systematic evaluation of genetic polymorphisms in carcinogen metabolizing enzymes associated with the risk of oral potentially malignant disorders occurrence 与口腔潜在恶性疾病发生风险相关的致癌物代谢酶遗传多态性的系统评价

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.09.010

Chao Lou , Yuhan Zhu , Linjun Shi , Wei Liu

Background/purpose

Accumulated studies investigate the association of single-nucleotide polymorphisms (SNPs) in carcinogen metabolizing enzymes with oral potentially malignant disorders (OPMD) risk. However, these results were inconsistent and conflicting. The purpose of this pooled analysis was to systematically evaluate the associations of SNPs in 6 enzymes (CYP1A1, CYP2E1, GSTM1, GSTM3, GSTT1 and GSTP1) with risk of OPMD occurrence.

Materials and methods

A systematic evaluation was performed to identify all eligible case ndash;control studies on the association between SNPs in 6 enzymes and OPMD onset. Odds ratios (ORs) and 95 % confidence intervals (CIs) were pooled to estimate association strength.

Results

A significant association of CYP2E1 PstI polymorphism with OPMD was found (OR, 1.46; 95%CI, 1.07–2.00) in 430 cases and 818 health controls. A significant association of GSTM1 null genotype with OPMD, especially oral leukoplakia, was found (OR, 1.72; 95%CI, 1.24–2.37) in 2228 cases and 4425 controls. A significant association of GSTT1 null genotype with OPMD, particularly oral submucous fibrosis, was found (OR, 1.50; 95%CI, 1.01–2.22) in 1798 cases and 3934 controls. A marginally significant association of GSTM3 polymorphism with OPMD was found (OR, 1.41; 95%CI, 1.00–1.98) in 321 cases and 622 controls. There was no significant association of polymorphisms in CYP1A1, CYP2E1 RsaI variant, and GSTP1 with OPMD.

Conclusion

This analysis for the first time investigated SNPs in carcinogen metabolizing enzymes with OPMD, suggesting that polymorphisms in CYP2E1 PstI, GSTM1, GSTM3 and GSTT1 play roles in OPMD occurrence and highlighting their potential in risk stratification and early detection strategies.

背景/目的积累的研究探讨了致癌物代谢酶的单核苷酸多态性（snp）与口腔潜在恶性疾病（OPMD）风险的关系。然而，这些结果是不一致和相互矛盾的。本合并分析的目的是系统评估6种酶（CYP1A1、CYP2E1、GSTM1、GSTM3、GSTT1和GSTP1）的snp与OPMD发生风险的关系。材料与方法系统评价所有符合条件的病例；6种酶snp与OPMD发病关系的对照研究。比值比（ORs）和95%置信区间（CIs）合并估计关联强度。结果430例患者和818例健康对照中发现CYP2E1 PstI多态性与OPMD有显著相关性（OR为1.46；95%CI为1.07 ~ 2.00）。在2228例病例和4425例对照中发现GSTM1零基因型与OPMD，特别是口腔白斑有显著相关性（OR, 1.72; 95%CI, 1.24-2.37）。在1798例病例和3934例对照中发现GSTT1零基因型与OPMD，特别是口腔黏膜下纤维化有显著相关性（OR, 1.50; 95%CI, 1.01-2.22）。在321例病例和622例对照中，GSTM3多态性与OPMD存在显著相关性（OR, 1.41; 95%CI, 1.00-1.98）。CYP1A1、CYP2E1 RsaI变异和GSTP1多态性与OPMD无显著相关性。结论本研究首次研究了OPMD致癌物代谢酶的snp，提示CYP2E1 PstI、GSTM1、GSTM3和GSTT1的多态性在OPMD的发生中发挥作用，并强调了其在风险分层和早期检测策略中的潜力。

{"title":"Systematic evaluation of genetic polymorphisms in carcinogen metabolizing enzymes associated with the risk of oral potentially malignant disorders occurrence","authors":"Chao Lou , Yuhan Zhu , Linjun Shi , Wei Liu","doi":"10.1016/j.jds.2025.09.010","DOIUrl":"10.1016/j.jds.2025.09.010","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Accumulated studies investigate the association of single-nucleotide polymorphisms (SNPs) in carcinogen metabolizing enzymes with oral potentially malignant disorders (OPMD) risk. However, these results were inconsistent and conflicting. The purpose of this pooled analysis was to systematically evaluate the associations of SNPs in 6 enzymes (CYP1A1, CYP2E1, GSTM1, GSTM3, GSTT1 and GSTP1) with risk of OPMD occurrence.</div></div><div><h3>Materials and methods</h3><div>A systematic evaluation was performed to identify all eligible case ndash;control studies on the association between SNPs in 6 enzymes and OPMD onset. Odds ratios (ORs) and 95 % confidence intervals (CIs) were pooled to estimate association strength.</div></div><div><h3>Results</h3><div>A significant association of CYP2E1 PstI polymorphism with OPMD was found (OR, 1.46; 95%CI, 1.07–2.00) in 430 cases and 818 health controls. A significant association of GSTM1 null genotype with OPMD, especially oral leukoplakia, was found (OR, 1.72; 95%CI, 1.24–2.37) in 2228 cases and 4425 controls. A significant association of GSTT1 null genotype with OPMD, particularly oral submucous fibrosis, was found (OR, 1.50; 95%CI, 1.01–2.22) in 1798 cases and 3934 controls. A marginally significant association of GSTM3 polymorphism with OPMD was found (OR, 1.41; 95%CI, 1.00–1.98) in 321 cases and 622 controls. There was no significant association of polymorphisms in CYP1A1, CYP2E1 RsaI variant, and GSTP1 with OPMD.</div></div><div><h3>Conclusion</h3><div>This analysis for the first time investigated SNPs in carcinogen metabolizing enzymes with OPMD, suggesting that polymorphisms in CYP2E1 PstI, GSTM1, GSTM3 and GSTT1 play roles in OPMD occurrence and highlighting their potential in risk stratification and early detection strategies.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 604-609"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145904243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term stability of implant site-switching technique and early orthodontic loading: A 16-year case report 种植体位置转换技术的长期稳定性与早期正畸负荷：16年病例报告

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.08.009

Tony Shing-Zeng Dung, Yuan-Yang Hsu, Cheng-Shan Li

引用次数: 0

The health screening measure for ionizing radiation workers under the labor insurance in Taiwan: A case study of dental assistant health management in a dental clinic 台湾劳动保险下电离辐射工作人员健康检查措施：以某牙科诊所牙科助理健康管理为例

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.08.030

Feng-Chou Cheng , Ling-Hsia Wang , Yu-Ling Wu, Chun-Pin Chiang

引用次数: 0

The evolution and future prospects of ionizing radiation protection training for dentists in Taiwan 台湾牙医电离辐射防护培训之发展与展望

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.08.036

Feng-Chou Cheng , Yu-Min Lin , Mu-Hsiung Chen, Chun-Pin Chiang

引用次数: 0

Applications and potential of ChatGPT in dentistry: Scoping review of research perspectives ChatGPT在牙科中的应用和潜力：研究前景的范围综述

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.08.016

Masakazu Hamada , Sumire Kikuchi , Tatsuya Akitomo , Satoru Kusaka , Yuko Iwamoto , Ryota Nomura

Since ChatGPT was released in 2022, it has been used in various settings and has rapidly been adopted worldwide. The main objective of this review was to provide a comprehensive assessment of the accuracy, reliability, usefulness, and limitations of ChatGPT in dentistry. PubMed, Scopus, and Web of Science databases were searched using the terms “ChatGPT” and “dentistry”, and 63 papers were extracted. Of the 63 records identified, medical consultation and assistance were the most common, accounting for 35 of 63 records (53.8 %), followed by study assistance and confirmation tests with 16 records (24.6 %), national and specialist dental examinations with 8 records (12.3 %), and dental research with 4 records (6.2 %). Although ChatGPT can offer many possibilities in the dental field, researchers and dentists should evaluate and use it carefully because ChatGPT answers are not always reliable, and there is a possibility of information leakage. Although its limitations need to be considered, ChatGPT has the potential to revolutionize dental diagnosis, treatment, education, and research.

自ChatGPT于2022年发布以来，它已在各种环境中使用，并在全球范围内迅速采用。本综述的主要目的是对ChatGPT在牙科中的准确性、可靠性、有用性和局限性进行全面评估。检索PubMed、Scopus和Web of Science数据库，检索词为“ChatGPT”和“dentistry”，共提取63篇论文。在确定的63份记录中，最常见的是医疗咨询和协助，占63份记录中的35份（53.8%），其次是学习协助和确认测试16份（24.6%），国家和专科牙科检查8份（12.3%），牙科研究4份（6.2%）。虽然ChatGPT在牙科领域提供了许多可能性，但研究人员和牙医应该仔细评估和使用它，因为ChatGPT的答案并不总是可靠的，并且存在信息泄露的可能性。虽然它的局限性需要考虑，ChatGPT有可能彻底改变牙科诊断，治疗，教育和研究。

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引用次数: 0

Biocompatibility and cytotoxic effects of myofunctional appliance materials on human periodontal ligament fibroblasts 肌功能矫治器材料对人牙周韧带成纤维细胞的生物相容性及细胞毒性作用

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.09.011

Tsui-Hsien Huang , Tso-Hsun Yang , Chuan-Yi Kao , Chun-Te Ho , Peerapong Santiwong , Chia-Tze Kao

Background/purpose

Prefabricated myofunctional devices are widely used in children's dentistry and early orthodontics. This study investigated the effects of materials used in myofunctional appliances on the viability of human periodontal ligament fibroblasts (HPLFs), inflammatory responses, and bone remodeling under simulated oral conditions. The focus was on biocompatibility and cytotoxicity to ensure safety in clinical applications.

Materials and methods

Four materials—EF Line (EF), ProOrtho (PO), Myobrace (MB), and Invisalign (IV)—were tested under conditions with and without artificial saliva (AS). HPLFs were cultured and exposed to eluates from these materials for 24, 48, and 72 h. Cell viability was measured using the MTT assay, and protein expression of inflammatory and bone remodeling markers (COX-2, IL-1, IL-6, TNF-α, ALP, OPG, RANKL) was evaluated using Western blotting.

Results

A 30 % AS concentration had minimal impact on cell viability and was used in subsequent experiments. EF showed significant cytotoxicity and elevated inflammatory protein expression, particularly IL-6 and COX-2, peaking at 48 and 72 h. PO exhibited moderate effects, while IV and MB maintained higher cell viability and lower inflammatory responses, similar to the control group. For bone remodeling markers, EF demonstrated high RANKL expression and low ALP/OPG levels, indicating bone resorption potential. In contrast, IV and MB had minimal impact on bone remodeling, maintaining a favorable RANKL/OPG ratio.

Conclusion

MB and IV demonstrated higher biocompatibility, minimal inflammatory effects, and stable bone remodeling properties. EF and PO exhibited higher cytotoxicity and inflammatory potential, maybe needed further material modifications to improve properties.

背景/目的预制肌功能装置广泛应用于儿童牙科和早期正畸治疗。本研究探讨了在模拟口腔条件下，肌功能矫具材料对人牙周韧带成纤维细胞（HPLFs）活力、炎症反应和骨重塑的影响。重点是生物相容性和细胞毒性，以确保临床应用的安全性。材料与方法在有人工唾液（AS）和无人工唾液（AS）条件下，对EF Line （EF）、ProOrtho （PO）、Myobrace （MB）和Invisalign (IV) 4种材料进行检测。培养HPLFs并将其暴露于这些材料的洗脱液中24、48和72小时。使用MTT法测量细胞活力，使用Western blotting评估炎症和骨重塑标志物（COX-2、IL-1、IL-6、TNF-α、ALP、OPG、RANKL）的蛋白表达。结果sa30 % AS浓度对细胞活力影响最小，可用于后续实验。EF表现出明显的细胞毒性，炎症蛋白表达升高，尤其是IL-6和COX-2，在48和72 h达到峰值。PO表现出中等效果，而IV和MB保持较高的细胞活力和较低的炎症反应，与对照组相似。对于骨重塑标志物，EF表现出高RANKL表达和低ALP/OPG水平，表明骨吸收潜力。相比之下，IV和MB对骨重塑的影响最小，保持了良好的RANKL/OPG比率。结论mb和IV具有较高的生物相容性、较小的炎症作用和稳定的骨重塑特性。EF和PO表现出较高的细胞毒性和炎症潜力，可能需要进一步的材料修饰来改善性能。

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引用次数: 0

Evaluation of poly-ε-caprolactone/graphene oxide three-dimensional porous scaffolds for potential application in alveolar bone repair 聚ε-己内酯/氧化石墨烯三维多孔支架在牙槽骨修复中的应用前景评价

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.08.045

Yung-Kang Shen , Huei-Yu Huang , Jowy Tani , Hsuan Chen , Silvia Anitasari , Hendrik Setia Budi , Wei-Chun Lin

Background/purpose

Alveolar bone reconstruction or regeneration demands the interim porous scaffold. It possesses adequate mechanical properties to enable it to withstand injured bones. These scaffolds are used to regulate the cell growth that migrate from surrounding tissue or are implanted within porous scaffold. This study is devoted to the manufacturing of 3D porous scaffolds and application on repair and regeneration of alveolar bone.

Materials and methods

Graphene oxide (GO) was mixed with poly-ε-caprolactone (PCL) material to fabricate 3D porous scaffolds by a solvent-casting/particulate-leaching method. The effects of various concentrations of GO (0.05, 0.1, 0.5, 1, and 2 wt.%) in PCL/GO scaffolds were focused on biological and physical properties. The human osteosarcoma cell (MG-63) in vitro was determined the biocompatibility of PCL/GO scaffolds.

Results

The PCL/GO scaffolds had the large porosity (greater than 88 %) in this study (P < 0.05). The Young's modulus of PCL/GO scaffold matched with human alveolar cancellous bone and it could be employed as the repair and support on this bone. The degradation rate of PCL/GO scaffolds was much lower than that of PCL scaffolds. The MG-63 cell displayed excellent attachment and proliferation on the PCL/GO scaffolds.

Conclusion

The 3D porous scaffold had an interconnected structure and its pore diameter was from 250 to 400 μm. Graphene oxide changes the surface properties of the 3D porous scaffold from hydrophobic to hydrophilic. The ALP assay indicated that MG-63 cell differentiated better in PCL/GO scaffolds containing 1 wt.% GO than on other scaffolds.

背景/目的牙槽骨重建或再生需要多孔支架。它具有足够的机械性能，使其能够承受受伤的骨头。这些支架用于调节从周围组织迁移或植入多孔支架内的细胞生长。本研究致力于三维多孔支架的制备及其在牙槽骨修复再生中的应用。材料与方法将氧化石墨烯（GO）与聚ε-己内酯（PCL）材料混合，采用溶剂铸造/颗粒浸出法制备3D多孔支架。不同浓度的氧化石墨烯（0.05、0.1、0.5、1和2 wt.%）对PCL/GO支架的生物学和物理性能的影响。体外测定人骨肉瘤细胞MG-63与PCL/GO支架的生物相容性。结果本研究制备的PCL/GO支架具有较大的孔隙率（大于88%）（P < 0.05）。PCL/GO支架的杨氏模量与人牙槽骨松质骨匹配，可作为牙槽骨松质骨的修复和支撑。PCL/GO支架的降解率远低于PCL支架。MG-63细胞在PCL/GO支架上表现出良好的附着和增殖能力。结论制备的三维多孔支架具有相互连接的结构，孔径在250 ~ 400 μm之间。氧化石墨烯将3D多孔支架的表面性质从疏水性变为亲水性。ALP实验表明，MG-63细胞在含有1 wt.%氧化石墨烯的PCL/GO支架中分化较好。

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引用次数: 0