Journal of Dental Sciences最新文献_第3页

Digital integration in dental education: A novel self-directed learning model using intraoral scanners for tooth preparation training

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.jds.2024.10.030

Chia-Ming Liu , Min-Hsun Hsu , Min Yee Ng , Chuan-Hang Yu

Background/purpose

Traditional dental education faces challenges, such as high student-to-faculty ratios and disruptions like the COVID-19 pandemic, which limit hands-on learning opportunities. Digital technologies, including intraoral scanners, offer potential solutions by improving accuracy and efficiency in clinical practice. This study explored the integration of digital tools in a self-directed learning model for the fixed prosthodontic tooth preparation.

Materials and methods

This study, conducted with 81 fourth-year dental students, incorporated digital tools like intraoral scanners into practical training. Students completed a pre-intervention evaluation, followed by training on digital analysis tools, and concluded with a self-directed learning protocol. The study assessed students' theoretical knowledge and practical skills using pre- and post-intervention tests, digital scans, and feedback questionnaires. Statistical analyses, including paired t-tests, evaluated the effectiveness of the intervention.

Results

Significant improvements were observed in both theoretical knowledge (pre-test 86 %, post-test 98 %, P = 0.012) and practical skills, with the percentage of “perfect” crown preparations rising from 14 % to 73 % (P < 0.0001). Occlusal reduction showed improvement but remained challenging for some students. Digital tools reduced student anxiety, with 77 % of students reporting decreased anxiety during practical exercises.

Conclusion

Integrating digital scanning technology with traditional teaching enhanced student competence in tooth preparation, reduced anxiety, and provided objective evaluation criteria. The self-directed learning model supported skill development and independent problem-solving, indicating the potential for broader application in dental education. Future research should explore long-term impacts on clinical performance and optimize digital tool integration throughout the curriculum.

背景/目的：传统的牙科教育面临着各种挑战，例如学生与教师的比例过高，以及 COVID-19 大流行等干扰因素，这些都限制了实践学习的机会。包括口内扫描仪在内的数字技术通过提高临床实践的准确性和效率提供了潜在的解决方案。本研究探讨了在固定义齿预备的自主学习模式中整合数字化工具的问题：本研究有 81 名四年级牙科学生参加，将口内扫描仪等数字化工具纳入了实践培训。学生们完成了干预前的评估，随后接受了数字化分析工具的培训，最后完成了自主学习协议。研究通过干预前后的测试、数字扫描和反馈问卷来评估学生的理论知识和实践技能。统计分析（包括配对 t 检验）评估了干预的效果：结果：理论知识（测试前为 86%，测试后为 98%，P = 0.012）和实践技能均有显著提高，牙冠准备 "完美 "的比例从 14% 提高到 73%（P 结论：通过对数字扫描技术与传统教学法的整合，牙科医师的理论知识和实践技能均有显著提高，牙冠准备 "完美 "的比例从 14% 提高到 73%：将数字扫描技术与传统教学相结合，提高了学生的备牙能力，减少了焦虑，并提供了客观的评价标准。自主学习模式支持技能发展和独立解决问题，这表明它有可能在牙科教育中得到更广泛的应用。未来的研究应探索对临床表现的长期影响，并优化整个课程中的数字工具整合。

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引用次数: 0

Sodium butyrate activates the extrinsic and intrinsic apoptotic processes in murine cementoblasts

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.jds.2024.11.006

Shih-Kai Lo , Ni-Yu Su , Chun-Chuan Su , Yu-Chao Chang

Background

/purpose: The metabolic by-product butyric acid of Gram-negative anaerobic bacteria can invoke pathological effects on periodontal cells resulting in inflammation and further destruction of periodontium. However, limited researches on the effects of butyric acid on cementoblasts were reported. Therefore, this study aimed to investigate the type of cell death in murine cementoblast (OCCM.30) caused by adding the different concentrations of sodium butyrate to the cell culture.

Materials and methods

OCCM.30 cells were exposed to sodium butyrate (0, 2, 4, 8, 16 mM) for 48 h. Cell viability was determined by microculture tetrazolium assay. Cell cycle distribution and cell death were analyzed by flow cytometry. Caspase-mediated apoptotic cascade was evaluated by Western blot.

Results

The concentrations of sodium butyrate≧4 mM were found to inhibit cell viability of OCCM.30 cells in a dose-dependent manner (P < 0.05). Sodium butyrate elevated sub-G1 cell population which exhibited cell apoptosis in OCCM.30 cells (P < 0.05). In addition, early and later apoptotic cells were found in sodium butyrate-induced cell death. Sodium butyrate significantly stimulated the degradation of procaspases-3, -8, and -9 levels, respectively (P < 0.05). Simultaneously, sodium butyrate corresponded to augment the levels of cleaved forms of caspases-3, -8, and -9, respectively (P < 0.05).

Conclusion

Taken together, sodium butyrate is a cytotoxic agent and can induce apoptosis on cementoblasts. The pathway involved in apoptosis is activated by caspase family signaling pathways. These evidences may provide a new mechanistic insight into the mechanism of damage of cementoblasts during the development and progression of periodontitis.

背景/目的：革兰氏阴性厌氧菌的代谢副产物丁酸会对牙周细胞产生病理影响，导致炎症和牙周进一步破坏：/目的：革兰氏阴性厌氧菌的代谢副产物丁酸会对牙周细胞产生病理影响，导致炎症和牙周的进一步破坏。然而，有关丁酸对骨水泥母细胞的影响的研究报道有限。因此，本研究旨在探讨向细胞培养物中添加不同浓度的丁酸钠对小鼠骨水泥母细胞（OCCM.30）造成的细胞死亡类型：将 OCCM.30 细胞置于丁酸钠（0、2、4、8、16 mM）中培养 48 小时。流式细胞术分析了细胞周期分布和细胞死亡情况。通过 Western 印迹对 Caspase 介导的细胞凋亡级联进行评估：结果：丁酸钠≧4 mM的浓度以剂量依赖性的方式抑制了OCCM.30细胞的活力（P P P P结论）：综上所述，丁酸钠是一种细胞毒剂，可诱导水泥母细胞凋亡。参与凋亡的途径是由 caspase 家族信号通路激活的。这些证据可能为牙周炎发生和发展过程中骨水泥母细胞的损伤机制提供了新的机制认识。

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引用次数: 0

Comparison of computed tomographic findings for radiolucent lesions of the mandibular ameloblastoma, odontogenic keratocyst, dentigerous cyst, and simple bone cyst 下颌骨釉母细胞瘤、牙源性角化囊肿、牙性囊肿和单纯骨囊肿放射状病变的计算机断层扫描结果比较

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.jds.2024.04.013

Tomoki Sueyoshi , Junsei Sameshima , Naoki Kaneko , Toru Chikui , Hu Chen , Shiho Yokomizo , Haruki Nagano , Taiki Sakamoto , Shintaro Kawano

Background/purpose

Radiolucent lesions of the mandible, including ameloblastoma, odontogenic keratocyst (OKC), dentigerous cyst (DC) and simple bone cyst (SBC), are frequently encountered in clinical practice. However, they vary in type and occasionally in appearance. Each lesion needs a different treatment and approach; therefore, accurate diagnosis is crucial before treatment. However, the radiographic findings, including computed tomography (CT), are often similar. This study aimed to compare the CT findings of 41 ameloblastomas, 74 OKCs, 87 DCs, and 13 SBCs in the mandible.

Materials and methods

Patients were evaluated on initial CT images focusing on features such as long/short diameters, relationship with adjacent teeth, cortex appearance, locularity, scalloped margins, and sclerotic rims. Multivariate logistic regression analysis was performed to determine the most useful features for differential diagnosis. Criteria for differential diagnosis were finally established for three lesions, excluding SBC, which had a relatively small number of cases.

Results

The relationship with the affected tooth, short diameters, sclerotic rims, and locularity were found to be significant features in the differential diagnosis. Ameloblastomas were characterized by relatively larger short diameters, frequently accompanied by root resorption and adjacent tooth displacement, while SBCs lacked these features. Sclerotic rims were commonly observed in OKCs and DCs, and most DCs were unilocular, containing a crown within the lesion. Based on these results, criteria were established for differential diagnosis with a diagnostic accuracy of 84.2%.

Conclusion

This is the first study to attempt to characterize each lesion's radiological features, and these criteria are likely to assist in clinical diagnosis.

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引用次数: 0

Beta-adrenergic receptor antagonist propranolol prevents bisphosphonate-related osteonecrosis of the jaw by promoting osteogenesis β-肾上腺素能受体拮抗剂普萘洛尔通过促进骨生成预防与双膦酸盐相关的颌骨坏死

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.jds.2024.04.016

Qianxin Du , Qizhang Wang , Yuhao Wang , Chengzhi Zhao , Jian Pan

Background/purpose

Bisphosphonate-related osteonecrosis of the jaw (BRONJ), a complication arising from the use of bisphosphonates (BPs), inflicts long-term suffering on patients. Currently, there is still a lack of effective treatments. This study aimed to explore the preventive effects of propranolol (PRO) on BRONJ in vitro and in vivo, given PRO's potential in bone health enhancement.

Materials and methods

In vitro, effect of PRO on zoledronic acid (ZA)-pretreated bone marrow mesenchymal stem cells (BMSCs) was detected by cell counting kit-8, alkaline phosphatase (ALP) staining, alizarin red staining, real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. In vivo, forty mice were divided into four groups: control, ZA, PRO, and ZA-PRO. The maxillary extraction sockets sides were analyzed with micro-CT and histomorphometry. Hematoxylin-eosin (H&E), Masson staining, immunofluorescence staining of ALP, bone morphogenetic protein 2 (BMP2), runt-related transcription factor 2 (RUNX2) and TUNEL staining were performed.

Results

PRO increased proliferation and osteogenic differentiation of BMSCs. PRO stimulated bone formation and facilitated the healing process in zoledronic acid-induced osteonecrosis of jaw in mouse model. Compared with ZA group, control and PRO group showed more BMP2⁺, RUNX2⁺, and ALP⁺ cells (P < 0.05). However, PRO rescued the decreased expression of ALP, RUNX2, BMP2 due to ZA and decreased the expression of TUNEL (P < 0.05).

Conclusion

The findings suggest that propranolol may offer a promising preventive strategy against BRONJ by enhancing bone regeneration. This research contributes to the understanding of the pathogenesis of BRONJ and opens avenues for potential treatments of BRONJ focusing on β-adrenergic signaling.

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引用次数: 0

A comparative analysis of older patients with chewing difficulties due to temporomandibular disorders and tooth loss 对因颞下颌关节紊乱和牙齿脱落导致咀嚼困难的老年患者进行比较分析

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.jds.2024.07.001

Hye-Mi Jeon , So-Yeun Kim , Yong-Woo Ahn , Soo-Min Ok , Sung-Hee Jeong , Hye-Min Ju

Background/purpose

Chewing difficulty can contribute to psychological stress, which reduces the quality of life for older adults. The purpose of this study was to investigate and analyze the severity of masticatory discomfort, stress response, and sleep disturbance in older patients experiencing masticatory discomfort due to tooth loss or temporomandibular disorders (TMD), to find the further treatment direction for these patients.

Materials and methods

A total of 392 patients aged 50 years and older with mastication difficulties were analyzed. Two group of patients, those seeking prosthetic treatment due to tooth loss (n = 193) and those who were referred due to TMD-related pain discomfort (n = 199), were identified. Numeric rating scale (NRS), stress response inventory (SRI), and insomnia severity index (ISI) were used as tools to measure patients’ subjective chewing discomfort, stress response, and sleep status.

Results

Chewing discomfort and age were found to be much higher in the prosthetic group than in the TMD group. The TMD group had significantly higher scores in five SRI parameters (tension, anger, depression, fatigue, and frustration) as well as total score and a higher percentage of clinical insomnia compared to prosthetic patients.

Conclusion

Since the number of remaining teeth has a significant impact on the masticatory discomfort severity, restoring the occlusion through prosthodontic treatment is important to improve masticatory function. In patients with TMD, it is necessary to assess stress response and sleep quality, and a multidisciplinary treatment approach may be necessary to effectively address masticatory discomfort.

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引用次数: 0

Evaluating the image recognition capabilities of GPT-4V and Gemini Pro in the Japanese national dental examination 评估 GPT-4V 和 Gemini Pro 在日本全国牙科考试中的图像识别能力

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.jds.2024.06.015

Hikaru Fukuda , Masaki Morishita , Kosuke Muraoka , Shino Yamaguchi , Taiji Nakamura , Izumi Yoshioka , Shuji Awano , Kentaro Ono

Background/purpose

OpenAI's GPT-4V and Google's Gemini Pro, being Large Language Models (LLMs) equipped with image recognition capabilities, have the potential to be utilized in future medical diagnosis and treatment, ands serve as valuable educational support tools for students. This study compared and evaluated the image recognition capabilities of GPT-4V and Gemini Pro using questions from the Japanese National Dental Examination (JNDE) to investigate their potential as educational support tools.

Materials and methods

We analyzed 160 questions from the 116th JNDE, administered in March 2023, using ChatGPT-4V, and Gemini Pro, which have image recognition functions. Standardized prompts were used for all LLMs, and statistical analysis was conducted using Fisher's exact test and the Mann–Whitney U test.

Results

For the 160 JNDE questions, the accuracy rates of GPT-4V and Gemini Pro were 35.0% and 28.1%, respectively, with GPT-4V being the highest, although not statistically significant. Across dental specialties, the accuracy rates of the GPT-4V were generally higher than those of the Gemini Pro, with some areas showing equal accuracy. Accuracy rates tended to decrease with an increased number of images within a question, suggesting that the number of images influenced the correctness of the responses.

Conclusion

The overall superior performance of GPT-4V compared to Gemini Pro may be attributed to the continuous updates in OpenAI's model. This research demonstrates the potential of LLMs as educational support tools in dentistry, while also highlighting areas that require further technological development.

{"title":"Evaluating the image recognition capabilities of GPT-4V and Gemini Pro in the Japanese national dental examination","authors":"Hikaru Fukuda , Masaki Morishita , Kosuke Muraoka , Shino Yamaguchi , Taiji Nakamura , Izumi Yoshioka , Shuji Awano , Kentaro Ono","doi":"10.1016/j.jds.2024.06.015","DOIUrl":"10.1016/j.jds.2024.06.015","url":null,"abstract":"<div><h3>Background/purpose</h3><div>OpenAI's GPT-4V and Google's Gemini Pro, being Large Language Models (LLMs) equipped with image recognition capabilities, have the potential to be utilized in future medical diagnosis and treatment, ands serve as valuable educational support tools for students. This study compared and evaluated the image recognition capabilities of GPT-4V and Gemini Pro using questions from the Japanese National Dental Examination (JNDE) to investigate their potential as educational support tools.</div></div><div><h3>Materials and methods</h3><div>We analyzed 160 questions from the 116th JNDE, administered in March 2023, using ChatGPT-4V, and Gemini Pro, which have image recognition functions. Standardized prompts were used for all LLMs, and statistical analysis was conducted using Fisher's exact test and the Mann–Whitney U test.</div></div><div><h3>Results</h3><div>For the 160 JNDE questions, the accuracy rates of GPT-4V and Gemini Pro were 35.0% and 28.1%, respectively, with GPT-4V being the highest, although not statistically significant. Across dental specialties, the accuracy rates of the GPT-4V were generally higher than those of the Gemini Pro, with some areas showing equal accuracy. Accuracy rates tended to decrease with an increased number of images within a question, suggesting that the number of images influenced the correctness of the responses.</div></div><div><h3>Conclusion</h3><div>The overall superior performance of GPT-4V compared to Gemini Pro may be attributed to the continuous updates in OpenAI's model. This research demonstrates the potential of LLMs as educational support tools in dentistry, while also highlighting areas that require further technological development.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 368-372"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141698741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COVID-19 vaccination patterns among oral cancer patients: A comprehensive analysis in a medical center in Taiwan 口腔癌患者的 COVID-19 疫苗接种模式：台湾一家医疗中心的综合分析

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.jds.2024.06.020

Chi-Sheng Cheng , Han-Pang Liu , Li-Tzu Lee

Background/purpose

COVID-19 vaccines are supplied at no-cost to residents as a measure to prevent comorbidities, fatalities, and the increased risk of community transmission, thus protecting public health systems. However, vaccine acceptance among cancer patients remained uncertain. This study aimed to elucidate the vaccination rates among oral cancer patients at a medical center in Taiwan.

Materials and methods

We included Individuals who attended for routine follow-ups from January 2021 to December 2022, with a total of 1448 patients subjected to comprehensive analysis. Medical records were reviewed to identify factors influencing vaccine acceptance.

Results

Our findings indicate that 1,264 patients received vaccinations, while 184 remained unvaccinated. The vaccination rates among patients in advanced disease stages and those with neck lymph node metastasis (N+) were significantly lower (both P < 0.001). Furthermore, a notable lower vaccination rate was evident among patients receiving active treatment modalities (P < 0.01). Conversely, age, and comorbidities (evaluated using the New Charlson Comorbidity Index), didn't show a significant correlation with vaccination rates. The risk of death caused by oral cancer among vaccinated patients was significantly lower compared to non-vaccinated group (P < 0.001). Logistic regression showed the risk of COVID infection was significant lower in vaccinated group than non-vaccinated group (OR = 0.31, P = 0.034).

Conclusion

The risk of COVID-19 infection in oral cancer patients was lower among vaccinated group, and the vaccination was not associated with more mortality. Identifying the characteristics of non-vaccinated individuals and understanding factors influencing vaccine hesitancy in oral cancer patients is vital for developing targeted strategies to improve vaccine uptake.

{"title":"COVID-19 vaccination patterns among oral cancer patients: A comprehensive analysis in a medical center in Taiwan","authors":"Chi-Sheng Cheng , Han-Pang Liu , Li-Tzu Lee","doi":"10.1016/j.jds.2024.06.020","DOIUrl":"10.1016/j.jds.2024.06.020","url":null,"abstract":"<div><h3>Background/purpose</h3><div>COVID-19 vaccines are supplied at no-cost to residents as a measure to prevent comorbidities, fatalities, and the increased risk of community transmission, thus protecting public health systems. However, vaccine acceptance among cancer patients remained uncertain. This study aimed to elucidate the vaccination rates among oral cancer patients at a medical center in Taiwan.</div></div><div><h3>Materials and methods</h3><div>We included Individuals who attended for routine follow-ups from January 2021 to December 2022, with a total of 1448 patients subjected to comprehensive analysis. Medical records were reviewed to identify factors influencing vaccine acceptance.</div></div><div><h3>Results</h3><div>Our findings indicate that 1,264 patients received vaccinations, while 184 remained unvaccinated. The vaccination rates among patients in advanced disease stages and those with neck lymph node metastasis (N+) were significantly lower (both P < 0.001). Furthermore, a notable lower vaccination rate was evident among patients receiving active treatment modalities (P < 0.01). Conversely, age, and comorbidities (evaluated using the New Charlson Comorbidity Index), didn't show a significant correlation with vaccination rates. The risk of death caused by oral cancer among vaccinated patients was significantly lower compared to non-vaccinated group (P < 0.001). Logistic regression showed the risk of COVID infection was significant lower in vaccinated group than non-vaccinated group (OR = 0.31, P = 0.034).</div></div><div><h3>Conclusion</h3><div>The risk of COVID-19 infection in oral cancer patients was lower among vaccinated group, and the vaccination was not associated with more mortality. Identifying the characteristics of non-vaccinated individuals and understanding factors influencing vaccine hesitancy in oral cancer patients is vital for developing targeted strategies to improve vaccine uptake.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 335-341"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141705906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matrix metalloproteinase-9 upregulation in keratinocytes of oral lichen planus via c-Jun N-terminal kinase signaling pathway activation 通过 c-Jun N 端激酶信号通路激活口腔扁平苔藓角质细胞中基质金属蛋白酶-9 的上调

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.jds.2024.07.010

Xinke Jiang , Yiwen Deng , Yirao Lai , Guanhuan Du , Xiye Li , Xiaojie Yang , Mingya Li , Lei Sun , Yufeng Wang , Guoyao Tang

Background/purpose

Oral lichen planus (OLP) is a chronic inflammatory disorder characterized by basement membrane disruption, which plays a crucial role in its pathogenesis. Matrix metalloproteinases (MMPs), a group of proteolytic enzymes, contribute to the degradation of the basement membrane. The specific MMPs secreted by keratinocytes in OLP lesions and relevant regulatory mechanisms are not fully understood. This study aimed to investigate the involvement of MMPs in OLP pathogenesis, focusing on their expression in keratinocytes and regulatory mechanisms.

Materials and methods

MMP mRNA expression in OLP epithelium was analyzed using RNA sequencing data obtained from the Gene Expression Omnibus (GEO) database. Mucosa samples from 30 OLP patients and 30 healthy controls were collected to observe the expression and regulation of MMPs in keratinocytes. The involvement of the mitogen-activated protein kinase (MAPK) pathway in MMP regulation was studied using HaCaT cells.

Results

RNA sequencing analysis revealed upregulation of MMP1 and MMP9 in OLP epithelium. MMP9 expression was predominantly observed in basal keratinocytes of OLP lesions. Elevated levels of phosphorylated c-Jun N-terminal kinase (JNK), a component of the MAPK pathway, were detected in OLP samples and co-localized with MMP9 in keratinocytes. Activation of the JNK pathway in HaCaT cells induced MMP9 expression, implicating JNK signaling in MMP9 regulation.

Conclusion

Keratinocytes contribute to OLP pathogenesis by secreting MMP9 through JNK pathway activation. This understanding may provide insights into targeted therapeutic interventions for this chronic recurrent disease.

{"title":"Matrix metalloproteinase-9 upregulation in keratinocytes of oral lichen planus via c-Jun N-terminal kinase signaling pathway activation","authors":"Xinke Jiang , Yiwen Deng , Yirao Lai , Guanhuan Du , Xiye Li , Xiaojie Yang , Mingya Li , Lei Sun , Yufeng Wang , Guoyao Tang","doi":"10.1016/j.jds.2024.07.010","DOIUrl":"10.1016/j.jds.2024.07.010","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Oral lichen planus (OLP) is a chronic inflammatory disorder characterized by basement membrane disruption, which plays a crucial role in its pathogenesis. Matrix metalloproteinases (MMPs), a group of proteolytic enzymes, contribute to the degradation of the basement membrane. The specific MMPs secreted by keratinocytes in OLP lesions and relevant regulatory mechanisms are not fully understood. This study aimed to investigate the involvement of MMPs in OLP pathogenesis, focusing on their expression in keratinocytes and regulatory mechanisms.</div></div><div><h3>Materials and methods</h3><div><em>MMP</em> mRNA expression in OLP epithelium was analyzed using RNA sequencing data obtained from the Gene Expression Omnibus (GEO) database. Mucosa samples from 30 OLP patients and 30 healthy controls were collected to observe the expression and regulation of MMPs in keratinocytes. The involvement of the mitogen-activated protein kinase (MAPK) pathway in MMP regulation was studied using HaCaT cells.</div></div><div><h3>Results</h3><div>RNA sequencing analysis revealed upregulation of <em>MMP1</em> and <em>MMP9</em> in OLP epithelium. MMP9 expression was predominantly observed in basal keratinocytes of OLP lesions. Elevated levels of phosphorylated c-Jun N-terminal kinase (JNK), a component of the MAPK pathway, were detected in OLP samples and co-localized with MMP9 in keratinocytes. Activation of the JNK pathway in HaCaT cells induced MMP9 expression, implicating JNK signaling in MMP9 regulation.</div></div><div><h3>Conclusion</h3><div>Keratinocytes contribute to OLP pathogenesis by secreting MMP9 through JNK pathway activation. This understanding may provide insights into targeted therapeutic interventions for this chronic recurrent disease.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 302-309"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141848711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-precision and non-invasive measurement of crestal bone level by optical coherence tomography

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.jds.2024.09.005

Wei-Ting Chang , Dong-Yuan Lyu , Yu-Lin Lai , Jui-Ying Yen , Ya-Chi Chen , Shyh-Yuan Lee

Background/purpose

Identifying crestal bone level (CBL) on the buccal and lingual aspects poses challenges in conventional dental radiographs. Given that optical coherence tomography (OCT) has the capability to non-invasively provide in-depth information about the periodontium, this in vitro study aimed to assess whether OCT can effectively identify periodontal landmarks and measure CBL in the presence of gingiva.

Materials and methods

An in-house handheld scanning probe connected to a 1310-nm swept-source OCT (SS-OCT) system, along with self-developed algorithms were employed to measure the CBL in dental models with artificial gingiva. Markers were positioned 0.5 mm above the artificial gingival margin (SG) and the crestal bone (SC) on both the mid-buccal and mid-lingual sides of 28 plastic teeth. The distances between the paired SG and SC were measured in the OCT images after correcting for the optical path through the covering artificial gingiva. These measurements were subsequently compared to the ground truth values obtained using a 2.5D inspection system.

Results

The mean difference in CBL measured by SS-OCT and 2.5D was 0.008 mm (95 % CI: −0.092 to 0.108 mm). Statistical analysis using a three-way ANOVA indicated that the measurement differences were not significant across maxillary/mandible, anterior/posterior, and buccal/lingual dimensions. Furthermore, these differences were not associated with gingival thickness (α = 0.05).

Conclusion

The proposed SS-OCT system demonstrated its capability to accurately and non-invasively assess CBL through artificial gingiva. Moreover, it facilitated the semi-automatic delineation of critical periodontal landmarks on OCT en face images, highlighting its potential for clinical applications.

{"title":"High-precision and non-invasive measurement of crestal bone level by optical coherence tomography","authors":"Wei-Ting Chang , Dong-Yuan Lyu , Yu-Lin Lai , Jui-Ying Yen , Ya-Chi Chen , Shyh-Yuan Lee","doi":"10.1016/j.jds.2024.09.005","DOIUrl":"10.1016/j.jds.2024.09.005","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Identifying crestal bone level (CBL) on the buccal and lingual aspects poses challenges in conventional dental radiographs. Given that optical coherence tomography (OCT) has the capability to non-invasively provide in-depth information about the periodontium, this in vitro study aimed to assess whether OCT can effectively identify periodontal landmarks and measure CBL in the presence of gingiva.</div></div><div><h3>Materials and methods</h3><div>An in-house handheld scanning probe connected to a 1310-nm swept-source OCT (SS-OCT) system, along with self-developed algorithms were employed to measure the CBL in dental models with artificial gingiva. Markers were positioned 0.5 mm above the artificial gingival margin (SG) and the crestal bone (SC) on both the mid-buccal and mid-lingual sides of 28 plastic teeth. The distances between the paired SG and SC were measured in the OCT images after correcting for the optical path through the covering artificial gingiva. These measurements were subsequently compared to the ground truth values obtained using a 2.5D inspection system.</div></div><div><h3>Results</h3><div>The mean difference in CBL measured by SS-OCT and 2.5D was 0.008 mm (95 % CI: −0.092 to 0.108 mm). Statistical analysis using a three-way ANOVA indicated that the measurement differences were not significant across maxillary/mandible, anterior/posterior, and buccal/lingual dimensions. Furthermore, these differences were not associated with gingival thickness (α = 0.05).</div></div><div><h3>Conclusion</h3><div>The proposed SS-OCT system demonstrated its capability to accurately and non-invasively assess CBL through artificial gingiva. Moreover, it facilitated the semi-automatic delineation of critical periodontal landmarks on OCT en face images, highlighting its potential for clinical applications.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 147-153"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphotungstic acid-enhanced micro-computed tomography and RNA sequencing provide a new perspective on temporomandibular joint arthritis induced by complete Freund's adjuvant and collagen-induced arthritis in rat models

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.jds.2024.08.014

Ding-Han Wang , Kim-Xuyen Nguyen , Trang Thi-Ngoc Tran , Po-Han Wu , Guang Hong , Yu-Min Lin , Yi-Chen Hsu , Cheng-Chieh Yang , Yu-Cheng Lin , Wun-Eng Hsu , Ming-Lun Hsu , Mu-Chen Yang

Background/Purpose

Temporomandibular joint (TMJ) arthritis causes inflammation and degradation of the mandibular condylar cartilage and subchondral bone. Complete Freund's adjuvant (CFA) and collagen-induced arthritis (CIA) are models for studying TMJ arthritis. While micro-computed tomography (micro-CT) is crucial for three-dimensional (3D) bone analysis, it has limitations in imaging nonmineralized tissues. Phosphotungstic acid (PTA) enhances soft tissue contrast. However, research on the 3D imaging of mandibular condylar cartilage and the molecular mechanisms of CFA- and CIA-induced arthritis remains unclear. This study aimed to investigate the bone and PTA-stained cartilage in the mandibular condyle using 3D reconstruction and explore the characteristics of enriched gene ontology terms underlying CFA- and CIA-induced TMJ arthritis in rat models.

Materials and methods

Rat mandibular condyles were collected from control, CFA, and CIA groups. Live micro-CT created 3D bone structures, and PTA-enhanced micro-CT constructed 3D mandibular condylar cartilage. Gene ontology enrichment analysis identified enriched gene ontology terms from differentially expressed genes through RNA sequencing.

Results

Major deformities in cartilage volume and bone morphology were observed in the arthritis-induced groups. The CIA group exhibited significant correlations between cartilage volume and bone parameters changes. Gene ontology enrichment analysis indicated fewer terms with upregulated differentially expressed genes related to inflammation and immune response in the CIA group than in the CFA group.

Conclusion

This study reveals distinct responses between CFA- and CIA-induced TMJ arthritis models. The CIA group exhibited strong correlations between cartilage volume and bone parameter changes and had less pronounced inflammation and immune response than the CFA group.

背景/目的：颞下颌关节（TMJ）关节炎会导致下颌骨髁状突软骨和软骨下骨的炎症和退化。完全弗氏佐剂（CFA）和胶原诱导关节炎（CIA）是研究颞下颌关节炎的模型。虽然显微计算机断层扫描（micro-CT）对三维（3D）骨分析至关重要，但它在非矿化组织成像方面存在局限性。磷钨酸（PTA）可增强软组织对比度。然而，下颌骨髁状突软骨的三维成像以及CFA和CIA诱发关节炎的分子机制研究仍不清楚。本研究旨在利用三维重建技术研究下颌骨髁状突软骨和PTA染色软骨，并探索大鼠模型中CFA和CIA诱导颞下颌关节炎的富集基因本体术语的特征：从对照组、CFA组和CIA组收集大鼠下颌骨髁突。活体显微 CT 绘制了三维骨结构，PTA 增强显微 CT 绘制了三维下颌骨髁状突软骨。基因本体富集分析通过 RNA 测序确定了差异表达基因的富集基因本体术语：结果：在关节炎诱发组中观察到软骨体积和骨形态的重大畸形。CIA组软骨体积和骨参数变化之间存在明显的相关性。基因本体富集分析表明，与 CFA 组相比，CIA 组中与炎症和免疫反应相关的差异表达基因上调的术语较少：本研究揭示了CFA和CIA诱导的颞下颌关节炎模型之间不同的反应。结论：本研究揭示了 CFA 和 CIA 诱导的颞下颌关节炎模型之间不同的反应，CIA 组软骨体积和骨参数变化之间具有很强的相关性，其炎症和免疫反应不如 CFA 组明显。

{"title":"Phosphotungstic acid-enhanced micro-computed tomography and RNA sequencing provide a new perspective on temporomandibular joint arthritis induced by complete Freund's adjuvant and collagen-induced arthritis in rat models","authors":"Ding-Han Wang , Kim-Xuyen Nguyen , Trang Thi-Ngoc Tran , Po-Han Wu , Guang Hong , Yu-Min Lin , Yi-Chen Hsu , Cheng-Chieh Yang , Yu-Cheng Lin , Wun-Eng Hsu , Ming-Lun Hsu , Mu-Chen Yang","doi":"10.1016/j.jds.2024.08.014","DOIUrl":"10.1016/j.jds.2024.08.014","url":null,"abstract":"<div><h3>Background/Purpose</h3><div>Temporomandibular joint (TMJ) arthritis causes inflammation and degradation of the mandibular condylar cartilage and subchondral bone. Complete Freund's adjuvant (CFA) and collagen-induced arthritis (CIA) are models for studying TMJ arthritis. While micro-computed tomography (micro-CT) is crucial for three-dimensional (3D) bone analysis, it has limitations in imaging nonmineralized tissues. Phosphotungstic acid (PTA) enhances soft tissue contrast. However, research on the 3D imaging of mandibular condylar cartilage and the molecular mechanisms of CFA- and CIA-induced arthritis remains unclear. This study aimed to investigate the bone and PTA-stained cartilage in the mandibular condyle using 3D reconstruction and explore the characteristics of enriched gene ontology terms underlying CFA- and CIA-induced TMJ arthritis in rat models.</div></div><div><h3>Materials and methods</h3><div>Rat mandibular condyles were collected from control, CFA, and CIA groups. Live micro-CT created 3D bone structures, and PTA-enhanced micro-CT constructed 3D mandibular condylar cartilage. Gene ontology enrichment analysis identified enriched gene ontology terms from differentially expressed genes through RNA sequencing.</div></div><div><h3>Results</h3><div>Major deformities in cartilage volume and bone morphology were observed in the arthritis-induced groups. The CIA group exhibited significant correlations between cartilage volume and bone parameters changes. Gene ontology enrichment analysis indicated fewer terms with upregulated differentially expressed genes related to inflammation and immune response in the CIA group than in the CFA group.</div></div><div><h3>Conclusion</h3><div>This study reveals distinct responses between CFA- and CIA-induced TMJ arthritis models. The CIA group exhibited strong correlations between cartilage volume and bone parameter changes and had less pronounced inflammation and immune response than the CFA group.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"20 1","pages":"Pages 189-200"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0