Journal of Dental Sciences最新文献_第2页

Fabricated citations in the age of AI: A wake-up call for editors, reviewers, and authors 人工智能时代的虚假引用：给编辑、审稿人和作者敲响的警钟

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.10.024

He Liu

引用次数: 0

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.07.031

Muhammad Usman Amanat , Hutcha Sriplung , Duangporn Kerdpon

Background/purpose

South Asia has a high burden of oral cancer (OC); however, delays in diagnosis remain under-researched. The study investigated delay intervals in OC diagnosis and the contributing factors in rural Pakistan, considering its unique sociocultural context.

Materials and methods

This multi-center cross-sectional study employed a structured questionnaire to interview 152 oral squamous cell carcinoma (OSCC) patients. The diagnostic intervals were determined using the Aarhus Statement. Logistic regression assessed the association between independent factors and delay types.

Results

Patient delays occurred in 76.3 % of cases, mostly due to appraisal delays (65 %), while diagnostic delays appeared in 51.3 %. Median durations for patient, diagnostic, and total delays were 3, 1, and over 4 months, respectively. Appraisal delay was associated with infrequent dental visits (adjusted odds ratio [AOR]: 11.04, confidence interval [CI]: 2.29–81.53), advanced stage OSCC (AOR: 5.42, CI: 2.35–13.03), and rural residence (AOR: 3.99, CI: 1.75–9.35). Help-seeking delay was linked to use of home remedies (AOR: 5.74, CI: 2.35–14.46) and homeopathy (AOR: 4.72, CI: 1.90–11.91). Patient delay associated with advanced stage OSCC (AOR: 7.73, CI: 3.28–19.12) and rural residence (AOR: 3.91, CI: 1.62–9.69). Diagnostic delay was influenced by patients’ lack of OC knowledge (AOR: 7.33, CI: 1.30–51.74), more than two visits before biopsy (AOR: 52.88, CI: 1.50–270.88), and initial treatment with analgesics (AOR: 13.37, CI: 3.68–60.99) or antimicrobials (AOR: 3.95, CI: 1.06–18.23).

Conclusion

Delays in OC diagnosis arise from inadequate patient awareness, rural residence, traditional and complementary medicine use, and health system challenges. Improving healthcare access and public awareness are crucial.

背景/目的南亚口腔癌（OC）负担高；然而，对诊断延误的研究仍然不足。考虑到巴基斯坦农村独特的社会文化背景，本研究调查了OC诊断的延迟间隔及其影响因素。材料与方法本研究采用结构化问卷对152例口腔鳞状细胞癌（OSCC）患者进行访谈。诊断间隔使用奥胡斯声明确定。Logistic回归评估独立因素与延迟类型之间的关系。结果有76.3%的病例出现延误，主要是由于评估延误（65%），而诊断延误（51.3%）。患者、诊断和总延迟的中位持续时间分别为3个月、1个月和4个月以上。评估延迟与不频繁牙科就诊（调整优势比[AOR]: 11.04，可信区间[CI]: 2.29-81.53）、晚期OSCC （AOR: 5.42, CI: 2.35-13.03）和农村居住（AOR: 3.99, CI: 1.75-9.35）相关。寻求帮助的延迟与使用家庭疗法（AOR: 5.74, CI: 2.35-14.46）和顺势疗法（AOR: 4.72, CI: 1.90-11.91）有关。患者延迟与晚期OSCC （AOR: 7.73, CI: 3.28-19.12）和农村居住（AOR: 3.91, CI: 1.62-9.69）相关。影响诊断延迟的因素有：患者对肿瘤缺乏了解（AOR: 7.33, CI: 1.30-51.74）、活检前两次以上就诊（AOR: 52.88, CI: 1.50-270.88）、初始治疗时使用止痛药（AOR: 13.37, CI: 3.68-60.99）或抗菌剂（AOR: 3.95, CI: 1.06-18.23）。结论慢性阻塞性肺疾病的诊断延误与患者认知不足、农村居住、传统和补充医学的使用以及卫生系统的挑战有关。改善医疗保健服务和提高公众意识至关重要。

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引用次数: 0

Assessment of osseointegration mechanisms in a novel zirconia implant: From experimental insights to clinical comparison 评估新型氧化锆种植体的骨整合机制：从实验见解到临床比较

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.08.018

Hung-Ying Lin , Yu-Ting Li , Ju-Hsuan Yang , Cheng-Han Chou , Hao-Hueng Chang , Chun-Pin Lin

Background/purpose

Zirconia has been proposed as a substitutive material for the next generation of implants. This study aimed to evaluate the osseointegration of a newly developed zirconia implant and compare its clinical parameters with titanium implants.

Materials and methods

Beagle dog animal model were used for evaluation of two groups of zirconia implants, with one group subjected to a newly developed sandblasting surface treatment technique and the other using a commercially available zirconia implant. The implants were randomly placed in bilateral edentulous mandibular sites and evaluated at 4, 9, and 13 weeks. The bone-to-implant contact (BIC) ratio was calculated and the fluorescence labelling for evaluation of osseointegration.

Clinical trials compared the peri-implant parameters of the zirconia implants with titanium implants to assess peri-implant tissue condition.

Results

Animal study indicated that zirconia implant osseointegration was observed between 4 weeks and 9 weeks, accompanied by osteoid deposition. A statistically significant difference was found between the 4-week and 13-week BIC ratio (P = 0.045). Clinical trials revealed that zirconia implants maintained minimal plaque postoperatively for up to three months, while titanium implants accumulated more plaque. In terms of plaque index, zirconia outperformed titanium implants.

Conclusion

This study provides comprehensive insights into zirconia implants. During early osseointegration, bone cells exhibited affinity for the implant surface, emphasizing the role of surface treatment. Clinical trials suggest that zirconia implants may offer a slight advantage in maintaining peri-implant periodontal conditions compared to titanium implants.

背景/目的氧化锆已被提出作为下一代植入物的替代材料。本研究旨在评估新开发的氧化锆种植体的骨整合性，并比较其与钛种植体的临床参数。材料与方法采用beagle犬动物模型对两组氧化锆种植体进行评价，一组采用新开发的喷砂表面处理技术，另一组采用市售氧化锆种植体。种植体随机放置在双侧无牙下颌部位，并在4、9和13周时进行评估。计算骨与种植体接触率（BIC），荧光标记评价骨整合。临床试验比较氧化锆种植体与钛种植体的种植体周围参数，评估种植体周围组织状况。结果动物实验表明，植入体氧化锆在4 ~ 9周出现骨整合，并伴有类骨沉积。4周与13周的BIC比值差异有统计学意义（P = 0.045）。临床试验显示，氧化锆种植体术后维持最小斑块长达3个月，而钛种植体积累更多斑块。在斑块指数方面，氧化锆优于钛种植体。结论本研究对氧化锆种植体提供了全面的认识。在早期骨整合过程中，骨细胞对种植体表面表现出亲和力，强调了表面处理的作用。临床试验表明，与钛种植体相比，氧化锆种植体在维持种植体周围牙周状况方面可能有轻微的优势。

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引用次数: 0

Oral and fecal microbiota in oral lichen planus and xerostomia patients: A preliminary study 口腔扁平苔藓和口干症患者的口腔和粪便微生物群：初步研究

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.09.018

Guan-Tiing Huang , Yu-Feng Huang

Background/purpose

The human microbiota constitutes a dynamic community of microorganisms inhabiting the body, with the gut and oral microbiotas being the most prominent. Previous studies have shown associations between oral microbiota disruption and various oral and systemic diseases, along with the involvement of the oral–gut microbiome axis. However, further investigation into the relationship between common oral conditions and microbiota changes remains needed. This study hypothesized that the distinct immune environments in oral lichen planus (OLP) and xerostomia patients result in recognizable microbiota compositions, with additional evaluation of fecal microbiota to explore the oral–gut axis.

Materials and methods

Gingival and fecal samples from 8 OLP patients, 19 xerostomia patients, and 10 healthy controls were collected and analyzed using 16S rRNA sequencing with bioinformatic analysis at the phylum level. Statistical comparisons between groups were performed using Student's T-test.

Results

Compared with healthy controls, OLP patients showed significant increases in Campylobacterota and Fusobacteria, and decreases in Actinobacteria and Proteobacteria. Xerostomia patients demonstrated a significant increase in Firmicutes. In fecal samples, both OLP and xerostomia patients exhibited significantly reduced Bacteroidetes compared with controls.

Conclusion

OLP and xerostomia are associated with distinct oral microbiota patterns, which may aid in early and non-invasive diagnosis. Fecal samples of both patient groups differed significantly from controls in Bacteroidetes, supporting the oral–gut microbiome axis and providing further evidence that oral conditions can influence systemic microbial communities. A major limitation of this study is the relatively small sample size.

背景/目的人体微生物群构成了一个动态的微生物群落，其中肠道和口腔微生物群最为突出。先前的研究表明，口腔微生物群破坏与各种口腔和全身疾病之间存在关联，以及口腔-肠道微生物群轴的参与。然而，对常见口腔状况和微生物群变化之间关系的进一步调查仍需进行。本研究假设口腔扁平苔藓（OLP）和口干症患者的不同免疫环境导致可识别的微生物群组成，并对粪便微生物群进行额外评估以探索口腔-肠道轴。材料与方法收集8例OLP患者、19例口干症患者和10例健康对照者的口腔和粪便样本，采用16S rRNA测序并进行门水平的生物信息学分析。组间比较采用Student’st检验。结果与健康对照组相比，OLP患者弯曲菌群和梭杆菌群明显增加，放线菌群和变形菌群明显减少。口干症患者表现出厚壁菌门的显著增加。在粪便样本中，与对照组相比，OLP和口干患者都显示出显着减少的拟杆菌群。结论olp和口干与不同的口腔菌群模式有关，有助于早期无创诊断。两组患者的粪便样本在拟杆菌门上与对照组有显著差异，这支持了口腔-肠道微生物组轴，并进一步证明口腔状况可以影响全身微生物群落。本研究的一个主要限制是样本量相对较小。

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引用次数: 0

Apoptosis-inducing factor mitochondria-associated 2 (AIFM2) promotes tumor progression and predicts poor prognosis in oral squamous cell carcinoma 凋亡诱导因子线粒体相关2 （AIFM2）促进口腔鳞状细胞癌的肿瘤进展并预测不良预后

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.11.007

Chung-Hsien Chou , Kuo-Wei Chang , Wan-Wen Hung , Miao-An Wei , Chung-Ji Liu , Shu-Chun Lin

Background/purpose

Head and neck squamous cell carcinoma (HNSCC), including oral squamous cell carcinoma (OSCC), remains a major malignancy with limited therapeutic efficacy. Apoptosis-inducing factor mitochondria-associated 2 (AIFM2), also known as ferroptosis suppressor protein 1, regulates ferroptosis and tumor progression. This study investigated the oncogenic function, clinical relevance, and regulation of AIFM2 in OSCC.

Materials and methods

Transcriptomic data from TCGA HNSCC and in-house OSCC RNA-Seq datasets were analyzed to assess AIFM2 expression and its association with clinicopathological features and outcomes. Functional assays evaluated the effects of AIFM2 knockdown or overexpression on OSCC cell proliferation, migration, invasion, and therapeutic response. MicroRNAs targeting AIFM2 were identified through bioinformatics, luciferase reporter, and mimic assays. A Light Gradient Boosting Machine (LGBM) model was used for prognostic prediction.

Results

AIFM2 overexpression was associated with advanced stage, poor tumor differentiation, and unfavorable survival in HNSCC/OSCC. AIFM2 knockdown suppressed, whereas its overexpression enhanced, OSCC cell proliferation, migration, and invasion, while exerting minimal effects on cisplatin, palbociclib, or cold atmospheric plasma sensitivity. miR-32-5p and miR-432-5p directly targeted AIFM2 and were downregulated in tumors. AIFM2-associated transcripts were enriched in pathways related to oxidative stress, lipid metabolism, and E2F targets. The LGBM-derived AIFM2 gene signature demonstrated strong prognostic predictive power.

Conclusion

AIFM2 acts as an oncogenic driver in OSCC, regulated by tumor-suppressive miR-32-5p and miR-432-5p, and serves as a potential prognostic biomarker and therapeutic target.

背景/目的头颈部鳞状细胞癌（HNSCC），包括口腔鳞状细胞癌（OSCC），仍然是一种治疗效果有限的主要恶性肿瘤。凋亡诱导因子线粒体相关2 (AIFM2)，也称为铁下垂抑制蛋白1，调节铁下垂和肿瘤进展。本研究探讨了AIFM2在OSCC中的致癌功能、临床意义及调控作用。材料和方法分析来自TCGA HNSCC和内部OSCC RNA-Seq数据集的转录组学数据，以评估AIFM2表达及其与临床病理特征和预后的关系。功能分析评估AIFM2敲低或过表达对OSCC细胞增殖、迁移、侵袭和治疗反应的影响。靶向AIFM2的microrna通过生物信息学、荧光素酶报告基因和模拟实验进行鉴定。采用光梯度增强机（LGBM）模型进行预后预测。结果aifm2过表达与HNSCC/OSCC的晚期、肿瘤分化差、生存不利相关。AIFM2敲低抑制OSCC细胞的增殖、迁移和侵袭，而其过表达增强，而对顺铂、帕博西尼或冷大气血浆敏感性的影响很小。miR-32-5p和miR-432-5p直接靶向AIFM2，在肿瘤中下调。aifm2相关转录本在氧化应激、脂质代谢和E2F靶点相关的途径中富集。lgbm衍生的AIFM2基因特征显示出很强的预后预测能力。结论aifm2在OSCC中是一种致癌驱动因子，受肿瘤抑制miR-32-5p和miR-432-5p的调控，可作为潜在的预后生物标志物和治疗靶点。

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引用次数: 0

APOBEC3B/ASF1B–TGF-β signaling axis promotes epithelial–mesenchymal transition in HPV-positive oropharyngeal cancer APOBEC3B/ ASF1B-TGF -β信号轴促进hpv阳性口咽癌上皮-间质转化

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.10.039

Ming-Shou Hsieh , Chih-Chi Sheen , Tung-Nien Hsu , Chi-Tai Yeh , Wei Jen Chang , Yang-Che Wu

Background/purpose

Human papillomavirus (HPV)-positive oropharyngeal cancer (OPC) generally shows better outcomes, yet a subset of patients develops aggressive lymphatic metastasis. The molecular determinants of this divergence remain unclear. This study explored the apolipoprotein B mRNA editing enzyme catalytic subunit 3B (APOBEC3B)/anti-silencing function 1B histone chaperone (ASF1B) axis as a potential mediator linking human papillomavirus (HPV) infection, DNA damage, and transforming growth factor beta (TGF-β) signaling in OPC.

Materials and methods

Transcriptomic and clinical datasets from The Cancer Genome Atlas (TCGA) were analyzed to examine correlations among APOBEC3B/ASF1B expression, HPV status, and TGF-β signaling activity. Functional assays using HPV-positive and HPV-negative cell models assessed APOBEC3B/ASF1B expression, replication protein A2 (RPA2) activation, and epithelial–mesenchymal transition (EMT) markers through quantitative polymerase chain reaction (qPCR), western blotting, and immunofluorescence.

Results

HPV infection markedly enhanced APOBEC3B and ASF1B expression, accompanied by RPA2 upregulation and EMT features. Silencing APOBEC3B reduced RPA2 and ASF1B levels and suppressed TGF-β signaling.

Conclusion

These findings identify APOBEC3B/ASF1B as a central pathway through which HPV infection activates TGF-β signaling and promotes EMT, offering potential biomarkers and therapeutic targets for high-risk HPV-positive OPC.

背景/目的人乳头瘤病毒（HPV）阳性口咽癌（OPC）通常表现出较好的预后，但一小部分患者会发生侵袭性淋巴转移。这种差异的分子决定因素尚不清楚。本研究探讨载脂蛋白B mRNA编辑酶催化亚基3B (APOBEC3B)/抗沉默功能1B组蛋白伴侣（ASF1B）轴在OPC中作为连接人乳头瘤病毒（HPV）感染、DNA损伤和转化生长因子β (TGF-β)信号传导的潜在介质。材料和方法对来自癌症基因组图谱（TCGA）的转录组学和临床数据集进行分析，以检验APOBEC3B/ASF1B表达、HPV状态和TGF-β信号活性之间的相关性。使用hpv阳性和hpv阴性细胞模型进行功能分析，通过定量聚合酶链反应（qPCR）、western blotting和免疫荧光检测APOBEC3B/ASF1B表达、复制蛋白A2 （RPA2）激活和上皮-间质转化（EMT）标志物。结果shpv感染显著增强APOBEC3B和ASF1B表达，并伴有RPA2上调和EMT特征。沉默APOBEC3B可降低RPA2和ASF1B水平，抑制TGF-β信号传导。结论APOBEC3B/ASF1B是HPV感染激活TGF-β信号并促进EMT的中心通路，为高危HPV阳性OPC提供了潜在的生物标志物和治疗靶点。

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引用次数: 0

A scientometric study on methylation modification and identification of related genes in oral potentially malignant disorders and oral cancer 口腔潜在恶性疾病和口腔癌中甲基化修饰和相关基因鉴定的科学计量学研究

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.07.024

Lifen Yin , Xiaoliang Luo , Jian Liu , Wei Liu , Liying Gu

Background/purpose

Accumulating evidence indicates that methylation modification alterations involve in the development and progression of oral cancer. The purpose of this study was to analyze the scientometric characteristics of methylation modification in oral potentially malignant disorders (OPMD) and oral squamous cell carcinoma (OSCC).

Materials and methods

All the papers on methylation research in OPMD/OSCC were comprehensively retrieved from the Scopus database with emphasis on the identification of methylation related genes.

Results

A total of 365 papers on methylation research in OPMD/OSCC were retrieved. The total citation count was 9998 and the h index was 53 for all the papers. The common keywords included prognosis, sensitivity and specificity, smoking, cohort analysis, saliva, receiver operating characteristic, follow up, risk factor, cancer diagnosis, tumor suppressor gene, biological marker. Among the 365 papers, a total of 542 methylated genes and 65 microRNAs were identified. The most common methylated gene was p16, followed by MGMT, DAPK/DAPK1, E-cadherin, RASSF1/RASSF1A, KIF1A, TIMP3, RUNX3, LINE1, CDH1, TERT, ZAP70, FLI1, GP1BB, and ZNF582. The most frequent methylation related microRNA was miR-296, followed by miR-193 and miR-137.

Conclusion

This study for the first time elucidated the scientometric characteristics of all the publications on methylation modification in oral carcinogenesis, and would provide new insights for researchers to comprehend the methylation specific gene profile related OPMD/OSCC.

背景/目的越来越多的证据表明甲基化修饰改变参与口腔癌的发生和进展。本研究的目的是分析甲基化修饰在口腔潜在恶性疾病（OPMD）和口腔鳞状细胞癌（OSCC）中的科学计量学特征。材料与方法综合检索Scopus数据库中所有关于OPMD/OSCC甲基化研究的论文，重点鉴定甲基化相关基因。结果共检索到OPMD/OSCC甲基化研究论文365篇。论文总被引次数9998次，h指数53。常见关键词包括预后、敏感性和特异性、吸烟、队列分析、唾液、受者操作特征、随访、危险因素、肿瘤诊断、肿瘤抑制基因、生物标志物。在这365篇论文中，共鉴定出542个甲基化基因和65个microrna。最常见的甲基化基因是p16，其次是MGMT、DAPK/DAPK1、E-cadherin、RASSF1/RASSF1A、KIF1A、TIMP3、RUNX3、LINE1、CDH1、TERT、ZAP70、FLI1、GP1BB和ZNF582。最常见的与甲基化相关的microRNA是miR-296，其次是miR-193和miR-137。结论本研究首次阐明了所有关于甲基化修饰在口腔癌变中的科学计量学特征，为研究人员理解与OPMD/OSCC相关的甲基化特异性基因谱提供了新的思路。

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引用次数: 0

Management of an endodontic lesion with secondary periodontal ligament changes in an adjacent tooth following root perforation: Two-year follow-up 根穿孔后邻牙伴继发性牙周韧带改变的根管病变处理：两年随访

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.09.002

Saeed Asgary

引用次数: 0

No evidence supporting a causal link between orthodontic premolar extraction and obstructive sleep apnea despite radiographic airway changes 没有证据支持正畸前磨牙拔牙和阻塞性睡眠呼吸暂停之间的因果关系，尽管x线片气道改变

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.08.033

Nia Ayu Ismaniati Noerhadi, Tsui-Hsein Huang, Chun-Te Ho, Chia-Tze Kao

引用次数: 0

Trimethyl chitosan: Antibacterial activity on Enterococcus faecalis biofilm and cytocompatibility on human periodontal ligament fibroblasts cells 三甲基壳聚糖：对粪肠球菌生物膜的抑菌活性及对人牙周韧带成纤维细胞的细胞相容性

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.06.022

Raras Ajeng Enggardipta , Minato Akizuki , Mika Bando , Yuji Inagaki , Kazumitsu Sekine , Kenichi Hamada , Tomoko Sumitomo , Kanta Sato , Hiromichi Yumoto

Background/purpose

Effective disinfection of the root canal system remains a major challenge due to complex anatomy and the persistence of biofilm-forming bacteria such as Enterococcus faecalis. Trimethyl chitosan (TMC), a quaternized chitosan derivative, has shown promising antimicrobial properties but has not been extensively studied for endodontic use. This study aimed to evaluate the antibacterial and antibiofilm efficacy of TMC against E. faecalis and assess its cytocompatibility with human periodontal ligament fibroblasts (HPdLFs).

Materials and methods

The E. faecalis biofilm formation in the presence of TMC and antibacterial activity of TMC against mature E. faecalis biofilms were evaluated using crystal violet staining, adenosine triphosphate assays, colony forming unit counting, scanning electron microscopy, and fluorescence microscopy. The expression of genes associated with E. faecalis biofilm formation, such as ace, esp, and gelE, was determined. Moreover, cytocompatibility of TMC with HPdLFs was assessed using a cell counting kit-8 assay.

Results

TMC significantly inhibited biofilm formation by E. faecalis and in the mature E. faecalis biofilm, TMC interfered with the total biofilm biomass, reduced bacterial numbers, weakened the biofilm structure, and upregulated ace, esp, and gelE expression. Furthermore, a lower concentration of TMC maintained HPdLFs viability.

Conclusion

This study highlights the potential of TMC as a novel irrigating material owing to its antibacterial and antibiofilm activities against E. faecalis and its cytocompatibility with HPdLFs.

背景/目的由于复杂的解剖结构和粪肠球菌等生物膜形成细菌的持续存在，根管系统的有效消毒仍然是一个主要挑战。三甲基壳聚糖（TMC）是一种季铵化壳聚糖衍生物，具有良好的抗菌性能，但在牙髓治疗中的应用还没有得到广泛的研究。本研究旨在评估TMC对粪肠杆菌的抗菌和抗生物膜作用，并评估其与人牙周韧带成纤维细胞（HPdLFs）的细胞相容性。材料与方法采用结晶紫染色、三磷酸腺苷测定、菌落形成单位计数、扫描电镜和荧光显微镜观察TMC对成熟粪肠球菌生物膜的抑菌活性及TMC对粪肠球菌生物膜形成的影响。测定与粪肠球菌生物膜形成相关的ace、esp、gelE等基因的表达。此外，使用细胞计数试剂盒-8测定TMC与HPdLFs的细胞相容性。结果TMC显著抑制粪肠球菌生物膜的形成，在成熟粪肠球菌生物膜中，TMC干扰生物膜总量，减少细菌数量，削弱生物膜结构，上调ace、esp和gelE的表达。此外，较低浓度的TMC维持了HPdLFs的活力。结论TMC对粪肠杆菌具有抗菌和抗生物膜活性，并具有与HPdLFs的细胞相容性，是一种新型的冲洗材料。

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引用次数: 0