Pub Date : 2024-08-06DOI: 10.1016/j.jds.2024.07.038
{"title":"Comment on: The relevance of dental management prior to radiation therapy with severe oral mucositis in head and neck cancer patients","authors":"","doi":"10.1016/j.jds.2024.07.038","DOIUrl":"10.1016/j.jds.2024.07.038","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1991790224002587/pdfft?md5=9f923b529ed0fa53d0f1195c972c3164&pid=1-s2.0-S1991790224002587-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bone reconstruction in the maxillofacial region typically relies on autologous bone grafting, which presents challenges, including donor site complications and graft limitations. Recent advances in tissue engineering have identified highly pure and proliferative dedifferentiated fat cells (DFATs) as promising alternatives. Herein, we explored the capacity for osteoblast differentiation and the osteoinductive characteristics of extracellular vesicles derived from DFATs (DFAT-EVs). DFATs were isolated from human buccal fat pads, cultured to confluency, and placed in either a standard or osteogenic induction medium. After culturing for 3 days, the conditioned medium was used to generate EVs using the size-exclusion chromatography and concentration filter method. Characterization of DFAT-EVs revealed typical EV morphology and positive markers (CD9 and CD63), with no differences between the two groups. assays demonstrated that EVs derived from the osteogenic induction medium (OI-EVs) significantly increased alkaline phosphatase activity and osteogenesis-related genes (Runx2 and collagen type I) compared to control EVs. Next-generation sequencing identified differentially expressed miRNAs, and gene ontology analysis suggested pathways involved in osteoblast differentiation. Isolating DFATs from buccal fat pads under osteogenic induction conditions offers a procedure confined to the oral cavity, eliminating the need for harvesting from other sites. Thus, DFAT-EVs hold promise for promoting bone regeneration in maxillofacial applications.
{"title":"Optimized human dedifferentiated fat cells from the buccal fat pad-derived osteoinductive extracellular vesicles promote osteoblast differentiation","authors":"Yusuke Nishiguchi, Mamoru Ueda, Hirohito Kubo, Jun-Ichiro Jo, Yoshiya Hashimoto, Toshihiko Takenobu","doi":"10.1016/j.jds.2024.07.028","DOIUrl":"https://doi.org/10.1016/j.jds.2024.07.028","url":null,"abstract":"Bone reconstruction in the maxillofacial region typically relies on autologous bone grafting, which presents challenges, including donor site complications and graft limitations. Recent advances in tissue engineering have identified highly pure and proliferative dedifferentiated fat cells (DFATs) as promising alternatives. Herein, we explored the capacity for osteoblast differentiation and the osteoinductive characteristics of extracellular vesicles derived from DFATs (DFAT-EVs). DFATs were isolated from human buccal fat pads, cultured to confluency, and placed in either a standard or osteogenic induction medium. After culturing for 3 days, the conditioned medium was used to generate EVs using the size-exclusion chromatography and concentration filter method. Characterization of DFAT-EVs revealed typical EV morphology and positive markers (CD9 and CD63), with no differences between the two groups. assays demonstrated that EVs derived from the osteogenic induction medium (OI-EVs) significantly increased alkaline phosphatase activity and osteogenesis-related genes (Runx2 and collagen type I) compared to control EVs. Next-generation sequencing identified differentially expressed miRNAs, and gene ontology analysis suggested pathways involved in osteoblast differentiation. Isolating DFATs from buccal fat pads under osteogenic induction conditions offers a procedure confined to the oral cavity, eliminating the need for harvesting from other sites. Thus, DFAT-EVs hold promise for promoting bone regeneration in maxillofacial applications.","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141969751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-03DOI: 10.1016/j.jds.2024.07.032
{"title":"Schwannoma of the tongue – A case report","authors":"","doi":"10.1016/j.jds.2024.07.032","DOIUrl":"10.1016/j.jds.2024.07.032","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1991790224002538/pdfft?md5=5190ae243088605645e06cd82100588a&pid=1-s2.0-S1991790224002538-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-03DOI: 10.1016/j.jds.2024.07.029
{"title":"The first dental clinic on a navy warship of Taiwan","authors":"","doi":"10.1016/j.jds.2024.07.029","DOIUrl":"10.1016/j.jds.2024.07.029","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1991790224002502/pdfft?md5=50bda404c4462cc94ccea647d1eeca74&pid=1-s2.0-S1991790224002502-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-03DOI: 10.1016/j.jds.2024.07.031
{"title":"Differential diagnoses for the bilateral multiple radiopaque materials superimposed in the panoramic and periapical radiographs","authors":"","doi":"10.1016/j.jds.2024.07.031","DOIUrl":"10.1016/j.jds.2024.07.031","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1991790224002526/pdfft?md5=e92869895af0f53daed8d52ed94bcf27&pid=1-s2.0-S1991790224002526-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-03DOI: 10.1016/j.jds.2024.07.030
Many people have associated e-cigarettes with adjectives such as trendy and cool, and regarded vaping as “a safer alternative to cigarette smoking”. Compared with the conventional cigarettes, the public is less familiar with the dangers of e-cigarettes. E-cigarettes produce an aerosol by heating the e-liquid (usually comprising nicotine, flavorings, propylene glycol, vegetable glycerin, and other additives). Harmful substances are generated during the vaporization, such as formaldehyde. Cellular damages and DNA hypermethylation are also found to be associated with vaping. We reviewed the clinical neoplastic cases of patients with vaping history (three oral squamous cell carcinomas, one conjunctival squamous intraepithelial neoplasia, and one thoracic NUT-midline carcinoma). These patients tend to be younger than the average cancer patients. Although the exact relations between the cellular damages or DNA hypermethylation and vaping still need further investigations, we should be very careful about the dangers of using e-cigarettes as aids for cigarette smoking cessation.
许多人将电子烟与时尚、酷等形容词联系在一起，认为电子烟是 "比吸烟更安全的替代品"。与传统香烟相比，公众对电子烟的危害不太了解。电子烟通过加热电子液体（通常包括尼古丁、香料、丙二醇、植物甘油和其他添加剂）产生气雾。气化过程中会产生甲醛等有害物质。细胞损伤和 DNA 高甲基化也与吸烟有关。我们回顾了有吸烟史患者的临床肿瘤病例（3 例口腔鳞状细胞癌、1 例结膜鳞状上皮内瘤变和 1 例胸部 NUT 中线癌）。这些患者往往比一般癌症患者年轻。虽然细胞损伤或DNA高甲基化与吸食电子烟之间的确切关系仍需进一步研究，但我们应该非常小心使用电子烟作为戒烟辅助工具的危险。
{"title":"Adverse effects of electronic cigarettes on human health","authors":"","doi":"10.1016/j.jds.2024.07.030","DOIUrl":"10.1016/j.jds.2024.07.030","url":null,"abstract":"<div><p>Many people have associated e-cigarettes with adjectives such as trendy and cool, and regarded vaping as “a safer alternative to cigarette smoking”. Compared with the conventional cigarettes, the public is less familiar with the dangers of e-cigarettes. E-cigarettes produce an aerosol by heating the e-liquid (usually comprising nicotine, flavorings, propylene glycol, vegetable glycerin, and other additives). Harmful substances are generated during the vaporization, such as formaldehyde. Cellular damages and DNA hypermethylation are also found to be associated with vaping. We reviewed the clinical neoplastic cases of patients with vaping history (three oral squamous cell carcinomas, one conjunctival squamous intraepithelial neoplasia, and one thoracic NUT-midline carcinoma). These patients tend to be younger than the average cancer patients. Although the exact relations between the cellular damages or DNA hypermethylation and vaping still need further investigations, we should be very careful about the dangers of using e-cigarettes as aids for cigarette smoking cessation.</p></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1991790224002514/pdfft?md5=5481f24f825a1709a7db3aa7feb01017&pid=1-s2.0-S1991790224002514-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-03DOI: 10.1016/j.jds.2024.07.034
{"title":"Clinical application of calcium sulfate for the augmentation of extraction socket with an oro-antral communication","authors":"","doi":"10.1016/j.jds.2024.07.034","DOIUrl":"10.1016/j.jds.2024.07.034","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1991790224002551/pdfft?md5=28bc582f4b07ee4832bd47d611cef56f&pid=1-s2.0-S1991790224002551-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-31DOI: 10.1016/j.jds.2024.07.024
{"title":"Medication-related osteonecrosis of the jaw in a patient with rheumatoid arthritis with suspected involvement of methotrexate and tocilizumab","authors":"","doi":"10.1016/j.jds.2024.07.024","DOIUrl":"10.1016/j.jds.2024.07.024","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1991790224002459/pdfft?md5=b2877a3e8c9689db0af1be612bcb7b94&pid=1-s2.0-S1991790224002459-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-29DOI: 10.1016/j.jds.2024.07.022
{"title":"Follicular dendritic cell sarcoma in the submandibular region: A report of a rare case","authors":"","doi":"10.1016/j.jds.2024.07.022","DOIUrl":"10.1016/j.jds.2024.07.022","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1991790224002435/pdfft?md5=2522f8ebc7e0f06a1209a73cf03819c3&pid=1-s2.0-S1991790224002435-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-26DOI: 10.1016/j.jds.2024.07.020
{"title":"Eradicating Enterococcus faecalis in root canal system: An update","authors":"","doi":"10.1016/j.jds.2024.07.020","DOIUrl":"10.1016/j.jds.2024.07.020","url":null,"abstract":"","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S199179022400240X/pdfft?md5=5bf0d5d0fcd5cfd67967c588e2c0ed3e&pid=1-s2.0-S199179022400240X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141849647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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