Journal of Dental Sciences最新文献_第6页

Adjuvant metronomic tegafur-uracil is associated with poorer outcomes and unfavorable histopathologic progression in oral squamous cell carcinomas 辅助节拍剂替加福-尿嘧啶与口腔鳞状细胞癌较差的预后和不利的组织病理学进展有关

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 Epub Date: 2025-11-12 DOI: 10.1016/j.jds.2025.10.023

Kai-Feng Hung , Yi-Ping Wang , Yi-Chen Sun , Zhi-Qian Lin , Cheng-Hsien Wu , Peter Mu-Hsin Chang , Julia Yu-Fong Chang

Background/purpose

Tegafur-uracil (UFUR) is widely prescribed as metronomic adjuvant chemotherapy for oral squamous cell carcinoma (OSCC) in East Asia, though its long-term benefit remains unclear. This study aimed to evaluate the oncologic and pathological impact of adjuvant UFUR in a large real-world OSCC cohort spanning three decades.

Materials and methods

This retrospective, single-institution cohort included 2048 patients with histopathologically confirmed OSCC treated at a tertiary medical center in Taiwan (1990–2020). All underwent curative-intent surgery with or without postoperative radiotherapy or concurrent chemoradiotherapy (CCRT). Among them, 878 patients received adjuvant metronomic UFUR for ≥12 months. Survival outcomes were analyzed using Kaplan–Meier and Cox proportional hazards models, and clinicopathologic associations were assessed using chi-square and Wilcoxon tests.

Results

Adjuvant UFUR did not improve disease-free survival (DFS) across cancer stages and was associated with significantly poorer DFS in early-stage diseases. Hazard ratios (HRs) for DFS in stages I–III were 1.523, 1.616, and 1.441, respectively (all P < 0.01). UFUR-treated patients also exhibited higher rates of recurrence, earlier onset of second primary cancers, and more frequent spindle-cell transformation, particularly among poorly-differentiated OSCCs.

Conclusion

Adjuvant metronomic UFUR provided no survival advantage and was associated with unfavorable histopathologic evolution in OSCCs. These findings warrant re-evaluation of UFUR as routine adjuvant therapy and support risk-adapted, molecularly guided postoperative strategies in oral cancer management.

背景/目的替加富-尿嘧啶（UFUR）在东亚地区被广泛用作口腔鳞状细胞癌（OSCC）的节律辅助化疗，但其长期疗效尚不清楚。本研究旨在评估辅助UFUR在30年真实世界大型OSCC队列中的肿瘤学和病理学影响。材料与方法本回顾性单机构队列研究纳入了2048例在台湾某三级医疗中心接受组织病理学证实的OSCC患者（1990-2020）。所有患者均接受了有或无术后放疗或同步放化疗（CCRT）的治疗目的手术。其中878例患者接受辅助节律性UFUR治疗≥12个月。使用Kaplan-Meier和Cox比例风险模型分析生存结果，使用卡方检验和Wilcoxon检验评估临床病理相关性。结果佐剂UFUR并没有改善癌症分期的无病生存（DFS），并且与早期疾病的无病生存（DFS）显著降低相关。I-III期DFS的风险比（hr）分别为1.523、1.616和1.441 （P均为0.01）。ufu治疗的患者也表现出更高的复发率，第二原发癌发病更早，纺锤细胞转化更频繁，特别是在低分化的OSCCs中。结论辅助节律性UFUR在OSCCs中没有生存优势，并与不利的组织病理演变有关。这些发现支持重新评估UFUR作为常规辅助治疗，并支持风险适应，分子指导的口腔癌术后治疗策略。

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引用次数: 0

Biocompatibility and cytotoxic effects of myofunctional appliance materials on human periodontal ligament fibroblasts 肌功能矫治器材料对人牙周韧带成纤维细胞的生物相容性及细胞毒性作用

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 Epub Date: 2025-09-24 DOI: 10.1016/j.jds.2025.09.011

Tsui-Hsien Huang , Tso-Hsun Yang , Chuan-Yi Kao , Chun-Te Ho , Peerapong Santiwong , Chia-Tze Kao

Background/purpose

Prefabricated myofunctional devices are widely used in children's dentistry and early orthodontics. This study investigated the effects of materials used in myofunctional appliances on the viability of human periodontal ligament fibroblasts (HPLFs), inflammatory responses, and bone remodeling under simulated oral conditions. The focus was on biocompatibility and cytotoxicity to ensure safety in clinical applications.

Materials and methods

Four materials—EF Line (EF), ProOrtho (PO), Myobrace (MB), and Invisalign (IV)—were tested under conditions with and without artificial saliva (AS). HPLFs were cultured and exposed to eluates from these materials for 24, 48, and 72 h. Cell viability was measured using the MTT assay, and protein expression of inflammatory and bone remodeling markers (COX-2, IL-1, IL-6, TNF-α, ALP, OPG, RANKL) was evaluated using Western blotting.

Results

A 30 % AS concentration had minimal impact on cell viability and was used in subsequent experiments. EF showed significant cytotoxicity and elevated inflammatory protein expression, particularly IL-6 and COX-2, peaking at 48 and 72 h. PO exhibited moderate effects, while IV and MB maintained higher cell viability and lower inflammatory responses, similar to the control group. For bone remodeling markers, EF demonstrated high RANKL expression and low ALP/OPG levels, indicating bone resorption potential. In contrast, IV and MB had minimal impact on bone remodeling, maintaining a favorable RANKL/OPG ratio.

Conclusion

MB and IV demonstrated higher biocompatibility, minimal inflammatory effects, and stable bone remodeling properties. EF and PO exhibited higher cytotoxicity and inflammatory potential, maybe needed further material modifications to improve properties.

背景/目的预制肌功能装置广泛应用于儿童牙科和早期正畸治疗。本研究探讨了在模拟口腔条件下，肌功能矫具材料对人牙周韧带成纤维细胞（HPLFs）活力、炎症反应和骨重塑的影响。重点是生物相容性和细胞毒性，以确保临床应用的安全性。材料与方法在有人工唾液（AS）和无人工唾液（AS）条件下，对EF Line （EF）、ProOrtho （PO）、Myobrace （MB）和Invisalign (IV) 4种材料进行检测。培养HPLFs并将其暴露于这些材料的洗脱液中24、48和72小时。使用MTT法测量细胞活力，使用Western blotting评估炎症和骨重塑标志物（COX-2、IL-1、IL-6、TNF-α、ALP、OPG、RANKL）的蛋白表达。结果sa30 % AS浓度对细胞活力影响最小，可用于后续实验。EF表现出明显的细胞毒性，炎症蛋白表达升高，尤其是IL-6和COX-2，在48和72 h达到峰值。PO表现出中等效果，而IV和MB保持较高的细胞活力和较低的炎症反应，与对照组相似。对于骨重塑标志物，EF表现出高RANKL表达和低ALP/OPG水平，表明骨吸收潜力。相比之下，IV和MB对骨重塑的影响最小，保持了良好的RANKL/OPG比率。结论mb和IV具有较高的生物相容性、较小的炎症作用和稳定的骨重塑特性。EF和PO表现出较高的细胞毒性和炎症潜力，可能需要进一步的材料修饰来改善性能。

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引用次数: 0

Evaluation of poly-ε-caprolactone/graphene oxide three-dimensional porous scaffolds for potential application in alveolar bone repair 聚ε-己内酯/氧化石墨烯三维多孔支架在牙槽骨修复中的应用前景评价

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 Epub Date: 2025-09-13 DOI: 10.1016/j.jds.2025.08.045

Yung-Kang Shen , Huei-Yu Huang , Jowy Tani , Hsuan Chen , Silvia Anitasari , Hendrik Setia Budi , Wei-Chun Lin

Background/purpose

Alveolar bone reconstruction or regeneration demands the interim porous scaffold. It possesses adequate mechanical properties to enable it to withstand injured bones. These scaffolds are used to regulate the cell growth that migrate from surrounding tissue or are implanted within porous scaffold. This study is devoted to the manufacturing of 3D porous scaffolds and application on repair and regeneration of alveolar bone.

Materials and methods

Graphene oxide (GO) was mixed with poly-ε-caprolactone (PCL) material to fabricate 3D porous scaffolds by a solvent-casting/particulate-leaching method. The effects of various concentrations of GO (0.05, 0.1, 0.5, 1, and 2 wt.%) in PCL/GO scaffolds were focused on biological and physical properties. The human osteosarcoma cell (MG-63) in vitro was determined the biocompatibility of PCL/GO scaffolds.

Results

The PCL/GO scaffolds had the large porosity (greater than 88 %) in this study (P < 0.05). The Young's modulus of PCL/GO scaffold matched with human alveolar cancellous bone and it could be employed as the repair and support on this bone. The degradation rate of PCL/GO scaffolds was much lower than that of PCL scaffolds. The MG-63 cell displayed excellent attachment and proliferation on the PCL/GO scaffolds.

Conclusion

The 3D porous scaffold had an interconnected structure and its pore diameter was from 250 to 400 μm. Graphene oxide changes the surface properties of the 3D porous scaffold from hydrophobic to hydrophilic. The ALP assay indicated that MG-63 cell differentiated better in PCL/GO scaffolds containing 1 wt.% GO than on other scaffolds.

背景/目的牙槽骨重建或再生需要多孔支架。它具有足够的机械性能，使其能够承受受伤的骨头。这些支架用于调节从周围组织迁移或植入多孔支架内的细胞生长。本研究致力于三维多孔支架的制备及其在牙槽骨修复再生中的应用。材料与方法将氧化石墨烯（GO）与聚ε-己内酯（PCL）材料混合，采用溶剂铸造/颗粒浸出法制备3D多孔支架。不同浓度的氧化石墨烯（0.05、0.1、0.5、1和2 wt.%）对PCL/GO支架的生物学和物理性能的影响。体外测定人骨肉瘤细胞MG-63与PCL/GO支架的生物相容性。结果本研究制备的PCL/GO支架具有较大的孔隙率（大于88%）（P < 0.05）。PCL/GO支架的杨氏模量与人牙槽骨松质骨匹配，可作为牙槽骨松质骨的修复和支撑。PCL/GO支架的降解率远低于PCL支架。MG-63细胞在PCL/GO支架上表现出良好的附着和增殖能力。结论制备的三维多孔支架具有相互连接的结构，孔径在250 ~ 400 μm之间。氧化石墨烯将3D多孔支架的表面性质从疏水性变为亲水性。ALP实验表明，MG-63细胞在含有1 wt.%氧化石墨烯的PCL/GO支架中分化较好。

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引用次数: 0

Laser-modified titanium surfaces induce sex-dimorphic secretion of angiogenic factors by gingiva-derived mesenchymal stromal cells 激光修饰钛表面诱导牙龈源性间充质间质细胞分泌血管生成因子

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 Epub Date: 2025-11-03 DOI: 10.1016/j.jds.2025.09.013

Ting-Han Chang , Hsin-Han Hou , Yi-Shing Shieh , Her-Hsiung Huang , Da-Yo Yuh

Background/purpose

Dental implants have been applied to restore missing dentition for more than fifty years. However, whether different surface modification on implant provide topographical cues that alter sex-specific effect on peri-implant angiogenesis remains unclear. This study aimed to investigate whether titanium surface modifications influence sex-specific chromatin accessibility, gene expression, and angiogenesis-related functions in human gingiva-derived mesenchymal stromal cells (GMSCs).

Materials and methods

We collected health gingival tissues from sixteen subjects (eight females and eight males) and isolated primary GMSCs. The primary GMSCs were cultured on three types of titanium surfaces: machined, lasered, and SLA (sand-blasted, large-grit, acid-etched). Chromatin accessibility and gene expression were assessed via ATAC-seq and RNA-seq, respectively, with further validation through qRT-PCR, ELISA, and western blot. Functional angiogenic potential was evaluated using both direct and indirect tube formation assays.

Results

Our findings revealed that titanium surface modifications induce sex-dimorphic effects on the secretion of angiogenic factors CCN1 and EDIL3. Laser-modified titanium significantly increased the secretion of extracellular vesicles enriched with both CCN1 and EDIL3 compared to machined surfaces. Interestingly, female GMSCs secreted higher levels of EDIL3, while male GMSCs secreted more CCN1 on lasered titanium surfaces. Moreover, lasered titanium demonstrated superior angiogenic potential in both direct and indirect tube formation assays compared with machined and SLA titanium.

Conclusion

This study suggests that laser-modified microchannels on titanium surfaces provide bioactive topographical cues that enhance the secretion of extracellular vesicles enriched with angiogenic factors—particularly CCN1 and EDIL3—by GMSCs, which may play key roles in promoting angiogenesis and supporting early-stage osseointegration.

背景/目的牙种植体用于修复缺失牙列已有50多年的历史。然而，不同的种植体表面修饰是否提供了改变种植体周围血管生成的性别特异性效应的地形线索尚不清楚。本研究旨在探讨钛表面修饰是否影响人牙龈源性间充质间质细胞（GMSCs）的性别特异性染色质可及性、基因表达和血管生成相关功能。材料和方法我们收集了16名受试者（男女各8名）的健康牙龈组织，并分离了原代GMSCs。原代GMSCs在三种钛表面培养：机械、激光和SLA（喷砂、大粒度、酸蚀）。分别通过ATAC-seq和RNA-seq评估染色质可及性和基因表达，并通过qRT-PCR、ELISA和western blot进一步验证。使用直接和间接的血管形成试验来评估功能性血管生成潜力。结果钛表面修饰可诱导血管生成因子CCN1和EDIL3分泌的性别二态效应。与加工表面相比，激光修饰钛显著增加了富含CCN1和EDIL3的细胞外囊泡的分泌。有趣的是，雌性GMSCs分泌更高水平的EDIL3，而雄性GMSCs在激光钛表面分泌更多的CCN1。此外，与机械钛和SLA钛相比，激光钛在直接和间接的血管生成实验中都表现出优越的血管生成潜力。结论激光修饰的钛表面微通道提供了生物活性的地形线索，可促进GMSCs分泌富含血管生成因子（特别是CCN1和edil3）的细胞外囊泡，这可能在促进血管生成和支持早期骨整合中发挥关键作用。

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引用次数: 0

Impact of female hormonal changes throughout life on oral health: A scoping review 一生中女性荷尔蒙变化对口腔健康的影响：范围综述

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 Epub Date: 2025-10-15 DOI: 10.1016/j.jds.2025.09.016

Inês Lopes Cardoso , Mayane Fryde , Maria Inês Guimarães , Fernanda Leal

This work looks at the impact of female hormonal fluctuations throughout life, during puberty, pregnancy and the menopause, on oral health. These physiological periods, marked by major variations in estrogen and progesterone levels, influence oral health, so the following question was formulated: to what extent do female hormonal fluctuations influence oral health? Through a scoping review of 37 scientific articles selected using a rigorous methodology (in particular using the Rayyan tool), it was possible to highlight clinical manifestations specific to each hormonal stage. In adolescent girls, puberty is frequently accompanied by exacerbated gingivitis, independent of dental plaque. During pregnancy, the increase in hormones leads to increased gingival inflammation and transient tooth mobility. Finally, the menopause is associated with a drop in female sex hormones, which can lead to dry mouth, altered salivary flora and accelerated bone loss, all of which contribute to the worsening of periodontal disease. These results underline the importance of a preventive and personalized approach to oral health care for patients, taking into account the hormonal context. They also highlight the collaboration between dentists, general practitioners and gynecologists, to optimize comprehensive care for women at every stage of their hormonal life.

这项研究着眼于女性荷尔蒙波动对口腔健康的影响，包括青春期、孕期和更年期。这些生理时期，以雌激素和黄体酮水平的主要变化为标志，影响口腔健康，因此提出了以下问题：女性荷尔蒙波动在多大程度上影响口腔健康？通过使用严格的方法（特别是使用Rayyan工具）对37篇科学文章进行范围审查，有可能突出每个激素阶段特有的临床表现。在青春期女孩中，青春期经常伴有牙龈炎的加重，与牙菌斑无关。在怀孕期间，荷尔蒙的增加会导致牙龈发炎和短暂的牙齿活动。最后，更年期与女性性激素下降有关，这会导致口干、唾液菌群改变和骨质流失加速，所有这些都会导致牙周病的恶化。这些结果强调了预防和个性化的方法对患者口腔保健的重要性，考虑到激素的背景。他们还强调了牙医、全科医生和妇科医生之间的合作，以优化妇女在荷尔蒙生活的每个阶段的综合护理。

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引用次数: 0

Plunging ranula with extension to the tongue base 下陷的ranula延伸到舌底

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 Epub Date: 2025-08-20 DOI: 10.1016/j.jds.2025.08.012

Satomi Sugiyama, Toshinori Iwai, Masaki Yoda, Kenji Mitsudo

引用次数: 0

Clinical decision support of advanced large language models in endodontic disease 高级大语言模型在牙髓疾病中的临床决策支持

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 Epub Date: 2025-06-20 DOI: 10.1016/j.jds.2025.06.007

Jiahe Li , Xian He , Yong Wang , Yanan Liu , Jie Liu , Mingxiao Liu , Tianyu Huang , Zeyu Huang

Background/purpose

Large language models (LLMs) exhibit significant potential for clinical decision support, yet their application in endodontic disease remains underexplored.

Materials and methods

This study assessed the decision-making capabilities of three advanced LLMs (GPT-4o, Claude 3.5, and Grok2) in specialized endodontic contexts. A question bank of 421 multiple-choice questions was constructed across 27 core endodontic topics, including theory, procedures, and 35 complex cases. The three LLMs were tested using standardized prompts, with performance evaluated via topic-stratified accuracy analysis.

Results

Claude 3.5 achieved the highest overall accuracy (73.39 %), followed by Grok2 (66.27 %) and GPT-4o (46.32 %). Grok2 excelled in complex case analysis (69.57 %). The models performed strongly in theoretical domains (e.g., clinical examination, structural function, pharmacology) but showed limitations in complex scenarios and procedural techniques.

Conclusion

LLMs hold promise as endodontic decision support tools, though domain-specific refinement is essential for effective clinical application.

背景/目的大型语言模型（LLMs）在临床决策支持方面显示出巨大的潜力，但它们在牙髓疾病中的应用仍未得到充分探索。材料和方法本研究评估了三种高级LLMs （gpt - 40, Claude 3.5和Grok2）在专业牙髓环境下的决策能力。题目库包含421道选择题，涉及27个牙髓学核心主题，包括理论、程序和35个复杂案例。三个llm使用标准化提示进行测试，并通过主题分层准确性分析评估性能。结果claude 3.5的总体准确率最高（73.39%），其次是Grok2（66.27%）和gpt - 40（46.32%）。Grok2在复杂病例分析方面表现突出（69.57%）。这些模型在理论领域（如临床检查、结构功能、药理学）表现良好，但在复杂场景和程序技术方面存在局限性。结论llm作为牙髓学决策支持工具具有良好的前景，但针对特定领域的改进对于有效的临床应用至关重要。

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引用次数: 0

Promoting dental students’ understanding of dental materials: A case study of dental materials laboratory education in Taiwan 促进牙科学生对牙科材料的了解：台湾牙科材料实验教育之个案研究

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 Epub Date: 2025-08-27 DOI: 10.1016/j.jds.2025.08.029

Ching-Shuen Wang , Feng-Chou Cheng , Wei-Chun Lin, Chun-Pin Chiang

引用次数: 0

Exploring existing methods for calculating dental arch form and curvature 探索计算牙弓形状和曲率的现有方法

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 Epub Date: 2025-09-13 DOI: 10.1016/j.jds.2025.08.046

Li-Tong Zheng, Sheng-Yang Lee, Yu-Chieh Lin, Wei-Chun Lin

引用次数: 0

A scientometric study on research trends and characteristics of mucoepidermoid carcinoma of salivary glands 唾液腺黏液表皮样癌研究趋势及特点的科学计量学研究

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 Epub Date: 2025-08-06 DOI: 10.1016/j.jds.2025.07.027

Xiuyin Wu , Yawen Ji , Ke Bi , Zhen Wang , Meili Sun

Background/purpose

Mucoepidermoid carcinoma (MEC) is the most common malignant tumor of the salivary glands. The purpose of this study was to analyze the scientometric characteristics and research trends of salivary MEC.

Materials and methods

All the papers on salivary MEC were comprehensively retrieved from the Scopus database. The years of publication were divided into before 2014 and Jan 2014–Jun 2025 in the analysis of research trends.

Results

There were 1308 papers on salivary MEC, with total citations of 24,598 and the h index of 70. The most frequent location of MEC involved was parotid gland, followed by minor saliva gland and submandibular gland. The keywords of differential diagnosis included adenosquamous carcinoma, pleomorphic adenoma, squamous cell carcinoma, Warthin tumor, and adenoid cystic carcinoma. Cancer surgery, radiotherapy, parotidectomy, neck dissection, chemotherapy, and cisplatin were the keywords of treatment. The research trend of has changed to cohort analysis, cancer prognosis, diagnostic imaging, positron emission tomography-computed tomography (PET-CT), perineural invasion, p63, gene mutation, gene rearrangement, and gene translocation after 2014. There have always been the common keywords such as pathology, tumor marker, immunohistochemistry, Ki-67, CRTC1, MAML2, gene fusion, and fluorescence in situ hybridization (FISH).

Conclusion

This study for the first time elucidated the current scenario and scientometric characteristics of MEC, and would help in improving in reciprocal collaboration and provide helpful guidance for further research.

背景/目的涎腺上皮样癌（MEC）是唾液腺最常见的恶性肿瘤。本研究的目的是分析唾液MEC的科学计量学特征和研究趋势。材料与方法从Scopus数据库中综合检索唾液MEC相关文献。在研究趋势分析中，将发表年份分为2014年以前和2014年1月- 2025年6月。结果共收录唾液MEC相关论文1308篇，总被引24598次，h指数为70。最常见的受累部位为腮腺，其次为小涎腺和下颌下腺。鉴别诊断关键词：腺鳞癌、多形性腺瘤、鳞状细胞癌、沃辛瘤、腺样囊性癌。肿瘤手术、放疗、腮腺切除术、颈部清扫、化疗、顺铂是治疗的关键词。2014年以后，研究趋势转变为队列分析、肿瘤预后、诊断影像、正电子发射断层扫描-计算机断层扫描（PET-CT）、神经周围侵袭、p63、基因突变、基因重排、基因易位。病理学、肿瘤标志物、免疫组织化学、Ki-67、CRTC1、MAML2、基因融合、荧光原位杂交（FISH）等常用关键词一直存在。结论本研究首次阐明了MEC的现状和科学计量学特征，有助于加强相互协作，为进一步的研究提供有益的指导。

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引用次数: 0