Pub Date : 2016-05-31DOI: 10.4172/2329-6631.1000E147
C. Soave, Maricela Viola-Rhenals, Y. Elghoul, Tao Peng, Q. Dou
Disulfiram (tetra ethyl thiuram disulfide, or DSF), is a disulfide derivative of N, N-diethyl dithiocarbamate (DEDTC). DSF is an FDA approved drug for the treatment of alcoholism, and at low doses continues to be a useful treatment option for those suffering from the disease. DSF is a member of the dithiocarbamate family, and is thus a metal chelator. Many cancer cells have elevated levels of copper, a metal to which DSF can bind. Thus, DSF is of great interest in cancer research as an antitumor agent, as studies suggest the DSF-copper complex may be cytotoxic to cancer cells. This could allow for targeting of some types of cancer cells with elevated copper levels by DSF.
{"title":"Repositioning an Old Anti-Alcoholism Drug: Disulfiram as a Selective,Effective and Economical Anticancer Agent","authors":"C. Soave, Maricela Viola-Rhenals, Y. Elghoul, Tao Peng, Q. Dou","doi":"10.4172/2329-6631.1000E147","DOIUrl":"https://doi.org/10.4172/2329-6631.1000E147","url":null,"abstract":"Disulfiram (tetra ethyl thiuram disulfide, or DSF), is a disulfide derivative of N, N-diethyl dithiocarbamate (DEDTC). DSF is an FDA approved drug for the treatment of alcoholism, and at low doses continues to be a useful treatment option for those suffering from the disease. DSF is a member of the dithiocarbamate family, and is thus a metal chelator. Many cancer cells have elevated levels of copper, a metal to which DSF can bind. Thus, DSF is of great interest in cancer research as an antitumor agent, as studies suggest the DSF-copper complex may be cytotoxic to cancer cells. This could allow for targeting of some types of cancer cells with elevated copper levels by DSF.","PeriodicalId":15589,"journal":{"name":"Journal of Developing Drugs","volume":"75 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2016-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88542240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-05-11DOI: 10.4172/2329-6631.C1.014
Reham F. El-Kased
S relationship is a key to the understanding of functioning of bioactive molecules, as well as a prerequisite for predictive analysis and design of novel molecules with desirable bioactive properties. The authors group had analysed various in silico methodologies (Ganguli and Sharma, Computer Society of India Communication, January 2016, pp 26—28) and the use of open source software packages. Use of open source software and databases permit molecular modeling and predictive design of molecules and of the active site in the protein by homolgy. Stability of a protein molecule in vivo determines the effectiveness of its function under physilogical conditions. Pathological conditions can be traced to malfunctioning of the proteins due the alteration in primary structure by mutation or by alteration in the post-translational modifications affecting functioning of the molecule. This review catalogues few of the better studied protein structures and describe their characteristic peculiarities with the hope that one would be able to develop predictive heuristics.
{"title":"Antibacterial activity of raw honey versus simulated honey solution","authors":"Reham F. El-Kased","doi":"10.4172/2329-6631.C1.014","DOIUrl":"https://doi.org/10.4172/2329-6631.C1.014","url":null,"abstract":"S relationship is a key to the understanding of functioning of bioactive molecules, as well as a prerequisite for predictive analysis and design of novel molecules with desirable bioactive properties. The authors group had analysed various in silico methodologies (Ganguli and Sharma, Computer Society of India Communication, January 2016, pp 26—28) and the use of open source software packages. Use of open source software and databases permit molecular modeling and predictive design of molecules and of the active site in the protein by homolgy. Stability of a protein molecule in vivo determines the effectiveness of its function under physilogical conditions. Pathological conditions can be traced to malfunctioning of the proteins due the alteration in primary structure by mutation or by alteration in the post-translational modifications affecting functioning of the molecule. This review catalogues few of the better studied protein structures and describe their characteristic peculiarities with the hope that one would be able to develop predictive heuristics.","PeriodicalId":15589,"journal":{"name":"Journal of Developing Drugs","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76336132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-05-11DOI: 10.4172/2329-6631.C1.013
Abdulaziz Saddique
{"title":"Pharmaceutical biotechnology manufacturing the future of medicine","authors":"Abdulaziz Saddique","doi":"10.4172/2329-6631.C1.013","DOIUrl":"https://doi.org/10.4172/2329-6631.C1.013","url":null,"abstract":"","PeriodicalId":15589,"journal":{"name":"Journal of Developing Drugs","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88272809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-04-09DOI: 10.4172/2329-6631.1000152
Sreelakshmy, Deepa Mk, P. Mridula
Glycyrrhiza glabra is a traditional herb which grows in various parts of the world, which have been used for the treatment of various diseases like gastric ulcer. Many reports have been published about the biogenesis of silver nanoparticles using Glycyrrhiza glabra, but green synthesized silver nanoparticles from Glycyrrhiza glabra has not yet been investigate the in-vitro anti-ulcer activity against H. pylori. In the present study was aimed to investigate the in-vitro anti-ulcer activity of green-synthesised silver nanoparticles (AgNPs) from Glycyrrhiza glabra root extract. The green synthesized of silver nanoparticles were characterized by UV-Visible Spectroscopy, X-ray diffraction (XRD), TEM, and FT-IR Analysis. UV-VIS Spectral analysis of the green synthesised nanoparticles was observed a sharp peak at 404 nm indicates the formation of silver nanoparticles. We successfully synthesized uniformly dispersed silver nanoparticles with a uniform size and shape in the range of 7 nm to 45 nm with an average size of 19 nm. The crystalline natures of Ag nanoparticles were confirmed from the XRD analysis. FTIR analysis was carried out to identify the possible biomolecules in Glycyrrhiza glabra root responsible for capping leading to efficient stabilization of the silver nanoparticles. The in-vitro antiulcer activities of synthesized silver nanoparticles were studied by Agar disc diffusion method and Micro broth dilution method. In Agar disc diffusion method showed the activity against H. pylori at the concentration of 500 μg/ml, which exhibit the most potent concentration of silver nanoparticles of gastric Cytoprotective anti-ulcer. In micro broth dilution method, The Minimum Inhibitory Concentration (MIC) of silver nanoparticles by visual examination was found to be 250 μg/ml.
{"title":"Green Synthesis of Silver Nanoparticles from Glycyrrhiza glabra Root Extract for the Treatment of Gastric Ulcer","authors":"Sreelakshmy, Deepa Mk, P. Mridula","doi":"10.4172/2329-6631.1000152","DOIUrl":"https://doi.org/10.4172/2329-6631.1000152","url":null,"abstract":"Glycyrrhiza glabra is a traditional herb which grows in various parts of the world, which have been used for the treatment of various diseases like gastric ulcer. Many reports have been published about the biogenesis of silver nanoparticles using Glycyrrhiza glabra, but green synthesized silver nanoparticles from Glycyrrhiza glabra has not yet been investigate the in-vitro anti-ulcer activity against H. pylori. In the present study was aimed to investigate the in-vitro anti-ulcer activity of green-synthesised silver nanoparticles (AgNPs) from Glycyrrhiza glabra root extract. The green synthesized of silver nanoparticles were characterized by UV-Visible Spectroscopy, X-ray diffraction (XRD), TEM, and FT-IR Analysis. UV-VIS Spectral analysis of the green synthesised nanoparticles was observed a sharp peak at 404 nm indicates the formation of silver nanoparticles. We successfully synthesized uniformly dispersed silver nanoparticles with a uniform size and shape in the range of 7 nm to 45 nm with an average size of 19 nm. The crystalline natures of Ag nanoparticles were confirmed from the XRD analysis. FTIR analysis was carried out to identify the possible biomolecules in Glycyrrhiza glabra root responsible for capping leading to efficient stabilization of the silver nanoparticles. The in-vitro antiulcer activities of synthesized silver nanoparticles were studied by Agar disc diffusion method and Micro broth dilution method. In Agar disc diffusion method showed the activity against H. pylori at the concentration of 500 μg/ml, which exhibit the most potent concentration of silver nanoparticles of gastric Cytoprotective anti-ulcer. In micro broth dilution method, The Minimum Inhibitory Concentration (MIC) of silver nanoparticles by visual examination was found to be 250 μg/ml.","PeriodicalId":15589,"journal":{"name":"Journal of Developing Drugs","volume":"38 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78503925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-04-08DOI: 10.4172/2329-6631.1000153
M. Karthikeyan, T. Balasubramanian, Pawan Kumar
Diabetes mellitus is a group of metabolic disorder, is characterized by absolute by lack of insulin and resulting in hyperglycemia. About 2.8% of global populations are affected by Diabetes mellitus. The search for new drug with new properties to treat the disease is still in progress. The current review have made an attempt to bring together all reported models and advanced techniques. Experimentally diabetes mellitus is generally induced in laboratory animals by several methods that include: chemical, surgical and genetic manipulations. The various in vitro techniques includes: In-vitro studies on insulin secretion, In-vitro studies on glucose uptake, Studies using isolated pancreatic islet cell lines, Assay of Amylase Inhibition and Inhibition of α-Glucosidase Activity. Experimental induction of diabetes mellitus in animal models and in vitro techniques are essentials for the advancement of our knowledge, clear understanding of pathogenesis and for finding new therapy. The animal models and in vitro techniques are essentials for developing a new drug for the treatment diabetes. More animal models, advanced techniques have to be developed for advances in diabetes research.
{"title":"In-vivo Animal Models and In-vitro Techniques for Screening Antidiabetic Activity","authors":"M. Karthikeyan, T. Balasubramanian, Pawan Kumar","doi":"10.4172/2329-6631.1000153","DOIUrl":"https://doi.org/10.4172/2329-6631.1000153","url":null,"abstract":"Diabetes mellitus is a group of metabolic disorder, is characterized by absolute by lack of insulin and resulting in hyperglycemia. About 2.8% of global populations are affected by Diabetes mellitus. The search for new drug with new properties to treat the disease is still in progress. The current review have made an attempt to bring together all reported models and advanced techniques. Experimentally diabetes mellitus is generally induced in laboratory animals by several methods that include: chemical, surgical and genetic manipulations. The various in vitro techniques includes: In-vitro studies on insulin secretion, In-vitro studies on glucose uptake, Studies using isolated pancreatic islet cell lines, Assay of Amylase Inhibition and Inhibition of α-Glucosidase Activity. Experimental induction of diabetes mellitus in animal models and in vitro techniques are essentials for the advancement of our knowledge, clear understanding of pathogenesis and for finding new therapy. The animal models and in vitro techniques are essentials for developing a new drug for the treatment diabetes. More animal models, advanced techniques have to be developed for advances in diabetes research.","PeriodicalId":15589,"journal":{"name":"Journal of Developing Drugs","volume":"9 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2016-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81379851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-24DOI: 10.4172/2329-6631.1000146
M. Stoicescu
The main objective of this clinical-case-presentation is to attract attention that the therapy with Gabaran in high dosage and per long period of time without a careful control of function of pancreas (common dosage of amilasemia and amilasuria level) can develop unexpected severe acute pancreatitis and may put the patient’s life in danger.
{"title":"Acute Pancreatitis after Therapy with GABARAN","authors":"M. Stoicescu","doi":"10.4172/2329-6631.1000146","DOIUrl":"https://doi.org/10.4172/2329-6631.1000146","url":null,"abstract":"The main objective of this clinical-case-presentation is to attract attention that the therapy with Gabaran in high dosage and per long period of time without a careful control of function of pancreas (common dosage of amilasemia and amilasuria level) can develop unexpected severe acute pancreatitis and may put the patient’s life in danger.","PeriodicalId":15589,"journal":{"name":"Journal of Developing Drugs","volume":"18 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2015-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75485679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-17DOI: 10.4172/2329-6631.1000145
Somia Gul, Sidra Sajid
Current study emphasis on development of an improved formulation of NFX HCl tablets that must be acceptable with reasonable limits of standards required for tablet. Central premise of research conducted was to evaluate and compare two available forms of Norfloxacin raw material, NFX base and another it’s acidic salt NFX HCl in tablet formulation and establish the effect of acidic and basic form of Norfloxacin. NFX base has been widely used in tablet formulation while Norfloxacin HCl is newly available form in market. After tablet preparation from each form, physical and chemical characterization was performed. Tablets formulated with Norfloxacin base complies with all specifications of physicochemical analysis whereas Norfloxacin HCl containing tablets exhibited twisted results in physical testing, increased hardness and disintegration. Thus an improved formulation was developed that could not interfere with physical as well as chemical characteristics. Result reveals that improved formulation with NFX HCl complies with standard of QC assessment.
{"title":"Formulation of Improved Norfloxacin HCl Tablets: Quality Control Assessment and Comparison Study of Acidic and Basic Form of Norfloxacin in Tablet Formulation","authors":"Somia Gul, Sidra Sajid","doi":"10.4172/2329-6631.1000145","DOIUrl":"https://doi.org/10.4172/2329-6631.1000145","url":null,"abstract":"Current study emphasis on development of an improved formulation of NFX HCl tablets that must be acceptable with reasonable limits of standards required for tablet. Central premise of research conducted was to evaluate and compare two available forms of Norfloxacin raw material, NFX base and another it’s acidic salt NFX HCl in tablet formulation and establish the effect of acidic and basic form of Norfloxacin. NFX base has been widely used in tablet formulation while Norfloxacin HCl is newly available form in market. After tablet preparation from each form, physical and chemical characterization was performed. Tablets formulated with Norfloxacin base complies with all specifications of physicochemical analysis whereas Norfloxacin HCl containing tablets exhibited twisted results in physical testing, increased hardness and disintegration. Thus an improved formulation was developed that could not interfere with physical as well as chemical characteristics. Result reveals that improved formulation with NFX HCl complies with standard of QC assessment.","PeriodicalId":15589,"journal":{"name":"Journal of Developing Drugs","volume":"20 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2015-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78550682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-07DOI: 10.4172/2329-6631.1000144
A. Hamza, T. Ksiksi, Obaid Ali Al Shamsi, Salem Abobakr Balfaqh
Inhibition of α-glucosiase and the associated reduction of glucose absorption is an attractive approach for decreasing postprandial hyperglycemia and for the discovery of potent antidiabetic agents. One of the most important sources of potential α-glucosiase inhibitors represents the class of polyphenols. This paper aims to evaluate previous herbal polyphenol-rich extracts plan on the management of diabetes mellitus, to address their α-glucosidase inhibitory activity. Polyphenol-rich extracts from thirteen widely used traditionally anti-diabetic plants in Asia and Mediterranean regions were evaluated for their potential α-glucosidaseinhibitory activity. Among these evaluated plants, 10 were much stronger than that of acarbose standard. Punica granatum manifested the highest inhibitory activity with IC50 at 3.59 ± 0.11 μg/mL, followed by Psidium guajava with IC50 at 8.08 ± 0.10 μg/mL and Cinnamomum zeylanicum with IC50 at 9.87.08 ± 0.14 μg/mLA. A high correlation (r=0.65, p<0.001) was observed between α-glucosidase inhibition and total phenolic content of all plants. Punica granatum, P. guajava, C. zeylanicum and Ziziphus spina-christi had also the highest total phenolic content. Extracts for the above studied plant species may potentially replace acarbose in its current clinical use in improving post-prandial glycaemic control in type 2 diabetics. As a result, these polyphenol-rich extracts potentially offer a complementary approach to develop functional food and potential antidiabetic agents.
{"title":"ñ-Glucosidase Inhibitory Activity of Common Traditional Medicinal PlantsUsed for Diabetes Mellitus","authors":"A. Hamza, T. Ksiksi, Obaid Ali Al Shamsi, Salem Abobakr Balfaqh","doi":"10.4172/2329-6631.1000144","DOIUrl":"https://doi.org/10.4172/2329-6631.1000144","url":null,"abstract":"Inhibition of α-glucosiase and the associated reduction of glucose absorption is an attractive approach for decreasing postprandial hyperglycemia and for the discovery of potent antidiabetic agents. One of the most important sources of potential α-glucosiase inhibitors represents the class of polyphenols. This paper aims to evaluate previous herbal polyphenol-rich extracts plan on the management of diabetes mellitus, to address their α-glucosidase inhibitory activity. Polyphenol-rich extracts from thirteen widely used traditionally anti-diabetic plants in Asia and Mediterranean regions were evaluated for their potential α-glucosidaseinhibitory activity. Among these evaluated plants, 10 were much stronger than that of acarbose standard. Punica granatum manifested the highest inhibitory activity with IC50 at 3.59 ± 0.11 μg/mL, followed by Psidium guajava with IC50 at 8.08 ± 0.10 μg/mL and Cinnamomum zeylanicum with IC50 at 9.87.08 ± 0.14 μg/mLA. A high correlation (r=0.65, p<0.001) was observed between α-glucosidase inhibition and total phenolic content of all plants. Punica granatum, P. guajava, C. zeylanicum and Ziziphus spina-christi had also the highest total phenolic content. Extracts for the above studied plant species may potentially replace acarbose in its current clinical use in improving post-prandial glycaemic control in type 2 diabetics. As a result, these polyphenol-rich extracts potentially offer a complementary approach to develop functional food and potential antidiabetic agents.","PeriodicalId":15589,"journal":{"name":"Journal of Developing Drugs","volume":"47 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2015-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76866115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-24DOI: 10.4172/2329-6631.1000143
Mária Äurišová
Objectives: The main objective of the current study was to present a further example which showed that a nontraditional mathematical modeling method can be advantageously used for the development of mathematical models in pharmacokinetics. An additional objective was to motivate researchers working in the field of pharmacokinetics to use a modeling method based on the theory of dynamic systems in their research work. Method: The current study is a companion piece of the earlier study by Kramer et al. Therefore the data published in the study cited here were used. As stated above, an advanced modeling method based on the theory of dynamic systems was employed. Conclusions: All mathematical models developed, successfully described the data of all volunteers investigated in the previous study by Kramer et al. and in the current study. The modeling method used in the current study is universal, comprehensive, and flexible. Therefore, it can be used to developed mathematical models not only in pharmacokinetics but also in several other scientific and practical fields.
{"title":"Mathematical Models of the Pharmacokinetic Behavior of Digoxin in FiveHealthy Subjects following Rapid Intravenous Injection of 1 mg of Digoxin","authors":"Mária Äurišová","doi":"10.4172/2329-6631.1000143","DOIUrl":"https://doi.org/10.4172/2329-6631.1000143","url":null,"abstract":"Objectives: The main objective of the current study was to present a further example which showed that a nontraditional mathematical modeling method can be advantageously used for the development of mathematical models in pharmacokinetics. An additional objective was to motivate researchers working in the field of pharmacokinetics to use a modeling method based on the theory of dynamic systems in their research work. Method: The current study is a companion piece of the earlier study by Kramer et al. Therefore the data published in the study cited here were used. As stated above, an advanced modeling method based on the theory of dynamic systems was employed. Conclusions: All mathematical models developed, successfully described the data of all volunteers investigated in the previous study by Kramer et al. and in the current study. The modeling method used in the current study is universal, comprehensive, and flexible. Therefore, it can be used to developed mathematical models not only in pharmacokinetics but also in several other scientific and practical fields.","PeriodicalId":15589,"journal":{"name":"Journal of Developing Drugs","volume":"22 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2015-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84520763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-20DOI: 10.4172/2329-6631.C1.011
Khalid Khan, Mohi Durakshan
Q based DOE approach was explored to study the effect of various factors influencing the optimisation of HPLC method for the simultaneous estimation of the four drugs viz. Ofloxacin (OFX), Ornidazole (ORN), Terbinafine Hydrochloride (TBH) and Clobetasol Propionate (CBP) in bulk drug and cream formulation. A full factorial design was employed to study the factors such as pH of the mobile phase, initial percentage of organic content for gradient elution (%BI) and gradient time (tG). The optimal conditions obtained after applying the principles of QbD with good system suitability parameters for all four drugs were found to be at pH 2.6, %BI as 24% of acetonitrile and gradient time of 4 min. The optimal conditions were found to be in a good agreement with the experimental results. The HPLC method thus developed was validated using ICH guidelines and was applied for the assay of cream formulation. The percentage recoveries were found to be 99.74±0.39 for OFX, 98.72±0.71 for ORN, 98.19±0.23 for TBH and 99.05±0.76 for CBP. The HPLC method was successfully applied to study the in vitro permeability of cream formulation in rat skin using Franz diffusion cell.
{"title":"HPLC method development and validation as per ICH guidelines","authors":"Khalid Khan, Mohi Durakshan","doi":"10.4172/2329-6631.C1.011","DOIUrl":"https://doi.org/10.4172/2329-6631.C1.011","url":null,"abstract":"Q based DOE approach was explored to study the effect of various factors influencing the optimisation of HPLC method for the simultaneous estimation of the four drugs viz. Ofloxacin (OFX), Ornidazole (ORN), Terbinafine Hydrochloride (TBH) and Clobetasol Propionate (CBP) in bulk drug and cream formulation. A full factorial design was employed to study the factors such as pH of the mobile phase, initial percentage of organic content for gradient elution (%BI) and gradient time (tG). The optimal conditions obtained after applying the principles of QbD with good system suitability parameters for all four drugs were found to be at pH 2.6, %BI as 24% of acetonitrile and gradient time of 4 min. The optimal conditions were found to be in a good agreement with the experimental results. The HPLC method thus developed was validated using ICH guidelines and was applied for the assay of cream formulation. The percentage recoveries were found to be 99.74±0.39 for OFX, 98.72±0.71 for ORN, 98.19±0.23 for TBH and 99.05±0.76 for CBP. The HPLC method was successfully applied to study the in vitro permeability of cream formulation in rat skin using Franz diffusion cell.","PeriodicalId":15589,"journal":{"name":"Journal of Developing Drugs","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87782678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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