Journal of Endocrinology and Reproduction最新文献

{"title":"Transient Neonatal-Onset Hypothyroidism Boosts Estradiol Synthesis in the Testis of Adult Wistar Rat","authors":"N. Venkatesh, M. K. Kumar, S. Sambavi, N. Kalpana, A. M. S. John, J. Anbalagan, R. Ilangovan, M. Aruldhas","doi":"10.18519/JER/2014/V18/67732","DOIUrl":"https://doi.org/10.18519/JER/2014/V18/67732","url":null,"abstract":"Transient neonatal hypothyroidism is known to boost Sertoli cell (SC), Leydig cell (LC) and germ cell (GC) number at adult age. Existing reports attribute decreased steroidogenic potential of LCs to unaltered/decreased serum testosterone in such rats, despite a boost in the cell number. However, these studies have ignored the status of estradiol in such conditions. In this present study, we tested the hypothesis \"transient neonatal-onset hypothyroidism may lead to a temporal shift in adult rat testicular steroidogenesis towards estradiol production\". Hypothyroidism was induced in neonates by providing methimazole (MMI) in drinking water (0.05%) to lactating mothers and pups for a transient period from postnatal day 1 (PND 1) to PND 14 or from PND 1 to PND 29. After the experimental period, the pups were provided drinking water free of MMI and sacrificed on PND 91. Coeval rats without MMI exposure served as control. Radioimmunoassay revealed decreased serum titres of luteinizing hormone (LH), follicle stimulating hormone (FSH), growth hormone (GH) and prolactin (PRL); while serum testosterone remained unaltered, its level in testicular interstitial fluid (TIF) decreased. Between the two major metabolites of testosterone, 5α-dihydrotestosterone (DHT) concentration decreased in serum and TIF, whereas estradiol recorded a significant increase in both. Transient neonatal-onset hypothyroidism decreased 3β-hydroxy steroid dehydrogenase (3β-HSD) in LCs but augmented 17β-HSD activity. Radio-receptor assay revealed decreased concentration of LHR and ER in LCs of hypothyroid groups, whereas AR and PRLR increased. While aromatase activity decreased in LCs, it increased along with FSHR in SCs, when compared to control rats. The changes in LHR and FSHR levels and aromatase were consistent with the expression level of the respective genes. The present study supports the proposed hypothesis and suggests that transient neonatal-onset hypothyroidism-induced boost in estradiol in adult rat testis may be due to augmented expression and activity of aromatase, and FSHR content in SCs of these rats.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"48 1","pages":"95-110"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91259019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Trigonella foenum Graecum (Fenugreek)-Loaded PLGA Nanoparticles on Non-Specific Esterase Enzyme Activity in Salivary Glands of Aging-Accelerated Male Mice","authors":"V. Deshmukh, M. Walvekar, S. S. Desai","doi":"10.18519/JER/2015/V19/86058","DOIUrl":"https://doi.org/10.18519/JER/2015/V19/86058","url":null,"abstract":"During oxidative stress, the reactive oxygen species (ROS) generally produce structural and biochemical alterations in cells and cell organelles. Impairment of lysosomal and mitochondrial function is the key for understanding the degenerative changes due to aging. Nonspecific esterase enzyme is particularly present in lysosomes and microsomes. In order to find the antioxidant property of fenugreek, non-specific esterase activity was measured to study the lysosomal membrane integrity in submandibular and sublingual salivary glands of D-galactose-induced aging male mice without and with supplementation of fenugreek. Adult male albino mice ( Mus musculus ) were divided in to six groups of five each and treated as follows: a) control for D-galactose (Dg) treatment; b) Dgtreated; c) Dg + fenugreek seed extract (FSE) treated concurrently; d) treated with Dg first, followed by treatment of FSE; e) Dg + FSE-loaded PLGA nanoparticle treated concurrently; and f) treated with Dg first followed by treatment of FSE-loaded PLGA nanoparticle. A significant decrease in non-specific esterase activity was observed in D-galactose treated mice, whereas FSE and FSE-PGLA groups of mice indicated protection against Dg-induced aging related oxidative stress in salivary glands. Thus, it is shown that fenugreek seed possesses antioxidant property, whereby non-specific esterase activity in salivary glands is increased but in a manner dependent on the treatment pattern, and the best result is obtained when FSE is administered post-treatment as loaded in PGLA nanoparticles.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"20 1","pages":"34-39"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82106840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuropeptide Y (NPY) Distribution in the Forebrain of Adult Spiny Eel, Macrognathus pancalus","authors":"M. Zahid, S. Malik, S. Rani","doi":"10.18519/JER/2014/V18/67734","DOIUrl":"https://doi.org/10.18519/JER/2014/V18/67734","url":null,"abstract":"In the present study, the distribution of neuropeptide Y (NPY)-immunoreactive neurons and fibers in the forebrain of adult spiny eel, Macrognathus pancalus , which is a bottom-dwelling nocturnal fish, was investigated. Serial Nissl-stained brain sections were used to demarcate forebrain regions and neuronal structures. NPY peptidecontaining cell bodies and fibers localized immunocytochemically were found widely distributed throughout the forebrain. The brain areas showing NPY distribution included predominant cell groups in the telencephalon (nucleus entopeduncularis, NE; nucleus of area ventralis telencephali, Vn), diencephalon (nucleus preopticus, pars parvocellularis, NPOp; nucleus preopticus, pars magnocellularis, NPOm; nucleus lateralis tuberis, NLT) and mesencephalon (midbrain tegmentum, MT). The important areas with only NPY-immunoreactive (-ir) fibers included olfactory bulb (OB), area dorsalis telencephali pars anterioris (Da), dorsal part of Dmd (Dmdd), ventral subdivision of Dl (Dlv), anterior subdivision of Dl (Dla), preoptic area (POA), optic tectum (OTec) and nucleus recessi lateralis (NRL). The pattern of NPY distribution in the forebrain of M. pancalus suggests its role in processing of many physiological functions (viz., feeding, daily activities, reproduction and other metabolic processes). The basic information on anatomical localization of NPY in eel will help to understand better the seasonal variations of NPY and its interaction with other reproductive hormones.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"95 1","pages":"75-86"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86361680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-Term in Situ Action of Melatonin on Ion Transport in Mice Kept at Restraint Stress","authors":"A. Khan, M. Peter","doi":"10.18519/JER/2015/V19/86052","DOIUrl":"https://doi.org/10.18519/JER/2015/V19/86052","url":null,"abstract":"Melatonin, a pleiotropic hormone, is involved in many physiological functions including combating oxidative stress. However, its role in ion transport during stress response is not yet understood. The dose-dependent effect of in situ melatonin was examined in Swiss albino mice. Perfusion of melatonin at 10 -7 M for 20 minutes produced a significant decrease in Na + , K + -ATPase activity in the kidney, liver, stomach and intestinal tissues. A dose-responsive decrease in cytosolic and mitochondrial H + ATPase activity was found in these tissues after melatonin perfusion. Likewise, the cytosolic and mitochondrial Ca 2+ ATPase activities decreased in the kidney, liver, stomach and intestine. The mitochondrial Mg 2+ ATPase activity decreased in the tested tissues in a dose-responsive manner. Subjecting mice to restraint stress for seven days increased the Na + , K + -ATPase, H + ATPase, Ca 2+ ATPase and Mg 2+ ATPase activities to significant levels in kidney, liver, stomach and intestinal tissues. On the contrary, in-situ perfusion of melatonin to stressed mice at 10 -9 M caused decrease in the stress-induced hyperactivity of these transmembrane ion transporters. These results provide evidence for a role of melatonin in ion transporter activity and point to a protective role of melatonin in ion transport during stress response in mice.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"36 1","pages":"20-33"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85209934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Constitutive Androstane Receptor (CAR): A Nuclear Receptor in Health and Disease","authors":"A. K. Dash, A. Yende, Sudhir Kumar, Shashi Singh, Deepak Kotiya, Manjul Rana, R. Tyagi","doi":"10.18519/JER/2014/V18/67731","DOIUrl":"https://doi.org/10.18519/JER/2014/V18/67731","url":null,"abstract":"Constitutive Androstane Receptor (CAR, NR1I3), a member of the nuclear receptor superfamily of transcription factors, has emerged as one of the key regulators of the drug and xenobiotic metabolism. The unique feature that separates CAR from other members of the superfamily is that it remains active in the absence of ligand and is further regulated by activators. From its first isolation in 1994, a number of studies related to its distribution, characteristics, functions, and relation to other members of the superfamily have been conducted that place it centrally, governing many key events of the body. Human CAR is expressed relatively higher in liver and epithelial cells of the small intestine villi and less in heart, muscle, kidney, brain and lung. Though there are some controversies regarding its subcellular localization in different cell lines, in general, the subcellular localization of CAR is reported to be predominantly cytoplasmic, in complex with co-chaperone partners HSP90 and CCRP (cytoplasmic CAR retention protein). To execute transcription functions, nuclear translocation is a prerequisite event for a NR, including CAR. In this context, existence of two pathways is suggested, i) direct mechanism of action; and ii) indirect mechanism of action that is governed via nuclear translocation of CAR. Additionally, existence of species-specific differences in its modulation with ligands acting either as an agonist, antagonist or inverse agonist is also apparent. Like the other xenobiotic receptor PXR, CAR also functions as an alternative 'xenosensor' to defend the body against persistent chemical insults. It responds to diverse array of chemically distinct compounds, including endobiotics and xenobiotics, to regulate the clearance of noxious chemicals and toxic metabolites in liver and intestine via induction of genes involved in their metabolism. The usefulness of targeting CAR in metabolic diseases including bilirubinemia, obesity, type 2 diabetes mellitus, atherosclerosis, preeclampsia, hypertension, cholestasis and also in liver cancer is being extensively studied in animal models. However, to determine the human relevance it requires further investigation. Though a large number of natural and synthetic compounds act as modulators of CAR, designing new derivatives with defined therapeutic benefit need to be investigated. The purpose of this review is to highlight the general aspects of nuclear receptor CAR, its mechanism of action and importance in human health and disease.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"33 1","pages":"59-74"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88265820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Gonadotropins and Gonadal Steroids (17β-Estradiol and Testosterone) on Harderian Gland Porphyrin Content and Circulatory Hormones in Indian Palm Squirrel Funambulus pennanti","authors":"S. Dubey, R. Verma, S. Rastogi, C. Haldar","doi":"10.18519/JER/2014/V18/71547","DOIUrl":"https://doi.org/10.18519/JER/2014/V18/71547","url":null,"abstract":"Mostly studied in the hamster, the Harderian gland (HG, an extra-orbital gland) has been shown to be capable of melatonin (MEL) synthesis and to possess steroid receptor binding sites. Sexual dimorphism and ability to respond to steroid hormones have been reported for HG of some temperate zone mammals. However, to date there is no report on functional relation of HG with pineal, pituitary and gonad in any rodent. Hence, we tested the effect of gonadotropins [FSH (10μg) + LH (10μg)] and steroids [testosterone (100μg) and 17-β estradiol (50μg)] on HG function in relation to the activities of the pineal gland and gonad in both sexes of Indian palm squirrel Funambulus pennanti during the sexually quiescent phase. In general, squirrels treated with gonadotropins and gonadal steroids showed a significant increase in gonadal weight and decrease in pineal weight and plasma melatonin level in LH- and testosterone-treated groups only. There was no change in the weight of HG on FSH treatment. Porphyrin content of HG decreased after testosterone and estradiol treatment. Pineal gland weight invariably decreased after treatment with gonadotropins and testosterone. Plasma testosterone and estradiol levels increased but melatonin level decreased in all treated groups. Our data suggest that in this tropical rodent gonadotropins have an indirect effect on HG and that gonadal steroids have significant effect on HG through the influence on gonadal and pineal functions.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"46 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86877238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Exogenous Gonadal Steroids on Reproductive Functions of the Indian Pygmy Field Mouse Mus terricolor","authors":"S. Arora, P. Singh, P. Basu, C. Haldar","doi":"10.18519/JER/2014/V18/71440","DOIUrl":"https://doi.org/10.18519/JER/2014/V18/71440","url":null,"abstract":"Different thresholds of gonadal steroids exert stimulatory or inhibitory effects on GnRH and gonadotropin release. During the reproductively active phase (RAP), the concentration of endogenous gonadal steroids remains high while, during reproductively inactive phase (RIP), it remains low. During RIP the HPG axis is sensitive to gonadal steroids but the circulatory levels of testosterone and estradiol remain low. During this phase one can observe conveniently the effects of gonadal steroids on the HPG axis in male or female rodents. Therefore, the aim of the present study was to find the effects of testosterone and estradiol on the reproductive functions of male and female Indian pygmy field mouse, Mus terricolor , during RIP. The male mice were injected aquaviron (commercially available testosterone, 1mg/100g body weight) while the female mice received estradiol-benzoate (25μg/100g body weight) for 15 consecutive days during the RIP. After completion of the treatment, a significant increase in the weights of gonads and accessory sex organs was noted in both the sexes. The biochemical constituents of accessory sex organs such as epididymal sialic acid, seminal vesicular fructose and uterine protein content reflected significant elevation accompanied by increased levels of plasma testosterone, estradiol and progesterone. Histologically, the gonads and accessory sex organs exhibited increased cellular activity. However, the gonadal cholesterol was significantly decreased in both the sexes. Over all, administration of gonadal steroids to both male and female mice accelerated the gonadal recrudescence but did not inhibit the reproductive functions when administered during the RIP. Therefore, it can be inferred from the present study that during the RIP the HPG axis is sensitive to gonadal steroids and, hence, exogenous gonadal steroids induce gonadal activity.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"20 1","pages":"29-36"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85912793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fertility Suppression by the Fruit Extract of Opuntia elatior in the Male Rat: Possible Extragonadal Action","authors":"Ramya M. Chamundaiah, Shivabasavaiah, T. Shivanandappa","doi":"10.18519/JER/2014/V18/71441","DOIUrl":"https://doi.org/10.18519/JER/2014/V18/71441","url":null,"abstract":"Plant - derived male antifertility agents need to address the basic requirements of reversibility and fertility suppression without affecting libido. This study was undertaken to evaluate the male antifertility potential of the fruit extract of Opuntia elatior in the male rat and its reversibility. The methanolic extract was administered through oral route (300, 900 mg/kg bw) to male rats for 30 and 60 days, and fertility was assessed by analysing spermatogenesis, sperm count and testosterone levels. Reversibility of fertility suppression was studied by withdrawal of treatment for two weeks and mating with normally cycling virgin females. Sperm count and motility were markedly reduced in both the groups of treated rats, without commensurate decline in serum testosterone levels and testicular hydroxysteroid dehydrogenase (HSDH) activity. Fertility of the treated rats was suppressed when mated with normally cycling virgin female rats without affecting libido. Withdrawal of treatment for two weeks restored the sperm count, testicular HSDH activity, serum testosterone levels and fertility. The methanolic extract of the fruit of O. elatior produces reversible male antifertility effect without affecting the testosterone levels and libido.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"15 1","pages":"7-16"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76691684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of L-tryptophan feeding on brain mitochondrial ion transport in net- confined climbing perch (Anabas testudineus Bloch)","authors":"S. Anupriya, V. S. Peter, M. Peter","doi":"10.18519/JER/2014/V18/71439","DOIUrl":"https://doi.org/10.18519/JER/2014/V18/71439","url":null,"abstract":"Serotonin (5-HT), a neurohormone with many physiological actions, is synthesized from the dietary essential amino acid tryptophan (TRP). However, the effects of TRP on neuronal ion transporters and its role in stress response have not yet been identified in the teleost fish. The effects of varied doses of TRP on the activities of mitochondrial (m) and cytosolic (c) ion transporters were examined in the forebrain (FB), midbrain (MB) and hindbrain (HB) segments of an air-breathing fish Anabas testudineus Bloch kept either in non-stressed or in stressed condition. Feeding the fish with varied doses of TRP (1, 2 and 4 mg g -1 feed) for seven days produced dose-dependent effects on Na + , K + -ATPase and H + -ATPase activities in different regions of fish brain. A decrease in (P<0.001) Na + , K + -ATPase activity was found in FB and MB after seven days of TRP treatment. TRP decreased (P<0.001) H + -ATPase activity in the FB but increased Na + , K + ATPase activity in all the three regions of the brain. In non-stressed fish, feeding 20 mg g -1 TRP for two days produced a substantial rise (P<0.001) in cH + -ATPase activity in the FB and HB of the fish. But mH + -ATPase showed a reversed response to TRP feeding. On the contrary, TRP treatment in net-confined fish showed a decrease (P<0.001) in the cH + -ATPase activity in the FB and HB, whereas it produced an increase (P<0.001) in the MB. In non-stressed fish, cytosolic and mitochondrial Ca 2+ -ATPase activities in FB and MB decreased (P<0.001) after TRP feeding. Feeding TRP in stressed fish reduced (P<0.001) cCa 2+ - ATPase activity in the MB but produced an increase (P<0.001) in its activity in mitochondria. In non-stressed fish, TRP feeding decreased (P<0.001) mMg 2+ -ATPase activity in the FB and MB segments. TRP treatment, in stressed fish, however decreased (P<0.001) Mg 2+ -ATPase activity in the MB but not in other brain segments. The data indicate that TRP can regulate brain mitochondrial ion transport, and induction of stress may modify the TRP-induced mitochondrial ion transport response of air-breathing fish.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"43 1","pages":"17-28"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82370743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-Term in Situ Effects of Prolactin and Insulin on Ion Transport in Liver and Intestine of Freshwater Climbing Perch ( Anabas testudineus Bloch)","authors":"M. Peter, V. Mini, D. S. Bindulekha, V. S. Peter","doi":"10.18519/JER/2014/V18/71719","DOIUrl":"https://doi.org/10.18519/JER/2014/V18/71719","url":null,"abstract":"The short-term in situ effects of prolactin and insulin on the transport of monovalent and divalent cations were examined in climbing perch Anabas testudineus to infer upon how these hormones regulate ion transport in the metabolic and osmoregualtory tissues of freshwater fish. Varied doses (10 -9 , 10 -8 and 10 -7 M) of ovine prolactin (oPRL) and insulin were infused in these fish for 20 min and the specific activities of ion-specific ATPases were analyzed. The Na + , K + -ATPase activity in the intestine and liver showed significant increase after oPRL infusion. Infusion of oPRL significantly decreased the cytosolic H + -ATPase activity in the intestine and liver, but increased the cytosolic Ca 2+ -ATPase activity in these tissues. Infusion of oPRL decreased the mitochondrial Mg 2+ -ATPase and H + -ATPase activities in the liver but the activities of these transporters increased in the intestine. Similar to oPRL, insulin infusion produced dose-dependent effects on the transporter activities in the liver and intestinal tissues. The mitochondrial Mg 2+ -ATPase activity in the liver significantly decreased at 10 -9 and 10 -7 M insulin infusion whereas its activity increased significantly in intestine at 10 -8 M insulin. Likewise, insulin infusion produced significant increases in the activities of all tested transporters except the cytosolic H + -ATPase activity in the intestine. A direct action of insulin on ion transport was found in both liver and intestinal tissues, as evident in the activity patterns of ion-specific ATPases. Taken together the results point to vital roles of PRL and insulin in ion transport in both liver and intestinal tissues of climbing perch.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"16 1","pages":"47-58"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88750669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}