Pub Date : 2020-03-03DOI: 10.1080/03601234.2019.1685318
A. A. Rigo, A. M. Cezaro, D. K. Muenchen, Janine Martinazzo, A. Manzoli, J. Steffens, C. Steffens
Abstract Heavy metals can be highly toxic depending on the dose and the chemical form. In this context, sensing devices such as nanobiosensors have been presented as a promising tool to monitor contaminants at micro and nanoscale. In this work, cantilever nanobiosensors with phosphatase alkaline were developed and applied to detect heavy metals (Pb, Ni, Cd, Zn, Co, and Al) in river water. The nanobiosensor surface was functionalized by the self-assembled monolayers (SAM) technique using 16-mercaptohexadecanoic acid, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) and N- hydroxysuccinimide (NHS), and phosphatase alkaline enzyme. The sensing layer deposited on the cantilever surface presented a uniform morphology, at nanoscale, with 80 nm of thickness. The nanobiosensor showed a detection limit in the ppb range and high sensitivity, with a stability of fifteen days. The developed cantilever nanobiosensor is a simple tool, suitable for the direct detection of contaminants in river water.
{"title":"Heavy metals detection in river water with cantilever nanobiosensor","authors":"A. A. Rigo, A. M. Cezaro, D. K. Muenchen, Janine Martinazzo, A. Manzoli, J. Steffens, C. Steffens","doi":"10.1080/03601234.2019.1685318","DOIUrl":"https://doi.org/10.1080/03601234.2019.1685318","url":null,"abstract":"Abstract Heavy metals can be highly toxic depending on the dose and the chemical form. In this context, sensing devices such as nanobiosensors have been presented as a promising tool to monitor contaminants at micro and nanoscale. In this work, cantilever nanobiosensors with phosphatase alkaline were developed and applied to detect heavy metals (Pb, Ni, Cd, Zn, Co, and Al) in river water. The nanobiosensor surface was functionalized by the self-assembled monolayers (SAM) technique using 16-mercaptohexadecanoic acid, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) and N- hydroxysuccinimide (NHS), and phosphatase alkaline enzyme. The sensing layer deposited on the cantilever surface presented a uniform morphology, at nanoscale, with 80 nm of thickness. The nanobiosensor showed a detection limit in the ppb range and high sensitivity, with a stability of fifteen days. The developed cantilever nanobiosensor is a simple tool, suitable for the direct detection of contaminants in river water.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"5 1","pages":"239 - 249"},"PeriodicalIF":0.0,"publicationDate":"2020-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91008283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-03DOI: 10.1080/03601234.2019.1675406
Jing Nie, P. Yaro, Kaifeng He, Hai-hong Hu, S. Zeng
Abstract The purpose of this study was to study the excretion stereoselectivity of triticonazole enantiomers in rat urine and faeces. Six male Sprague-Dawley (SD) rats were administrated 50 mg/kg rac-triticonazole. Rats urine and faeces were separately and quantitatively collected at the following intervals: 0–3, 3–6, 6–9, 9–12, 12–24, 24–36 and 36–48 h. The faeces samples were homogenized in an aqueous solution containing 0.2% DMSO at the ratio of 1 g: 40 mL. An aliquot of 100 μL rats urine or faeces homogenate was spiked and mixed with 6.0 μL of 1.00 μg/mL flusilazole as an internal standard. The triticonazole enantiomers in urine and faeces were determined by using an HPLC/MS–MS after samples preparation. The excreted amounts of enantiomers in the urine showed a significant difference (P < 0.05) except for 3–6 h. The cumulative excretion rate (Xu0→24) in urine was 26.43 ± 0.08% and 37.58 ± 0.11% for R-(−)- and S-(+)-triticonazole, respectively, indicating high enantioselectivity (P < 0.001). The cumulative excretion rate (Xu0→72) in faeces was 6.93 ± 0.03% and 6.77 ± 0.03% for R-(−)- and S-(+)-triticonazole, respectively, without a difference. The results showed that the total cumulative percentage of triticonazole enantiomers accounted for in urine and faeces was 64.00 ± 0.13% and 13.70 ± 0.32%, the urinary excretion of R-(−)- and S-(+)-triticonazole were significantly different and S-(+)-triticonazole was preferentially excreted. However, the faecal excretion of the enantiomers showed no difference.
{"title":"Excretion stereoselectivity of triticonazole in rat urine and faeces","authors":"Jing Nie, P. Yaro, Kaifeng He, Hai-hong Hu, S. Zeng","doi":"10.1080/03601234.2019.1675406","DOIUrl":"https://doi.org/10.1080/03601234.2019.1675406","url":null,"abstract":"Abstract The purpose of this study was to study the excretion stereoselectivity of triticonazole enantiomers in rat urine and faeces. Six male Sprague-Dawley (SD) rats were administrated 50 mg/kg rac-triticonazole. Rats urine and faeces were separately and quantitatively collected at the following intervals: 0–3, 3–6, 6–9, 9–12, 12–24, 24–36 and 36–48 h. The faeces samples were homogenized in an aqueous solution containing 0.2% DMSO at the ratio of 1 g: 40 mL. An aliquot of 100 μL rats urine or faeces homogenate was spiked and mixed with 6.0 μL of 1.00 μg/mL flusilazole as an internal standard. The triticonazole enantiomers in urine and faeces were determined by using an HPLC/MS–MS after samples preparation. The excreted amounts of enantiomers in the urine showed a significant difference (P < 0.05) except for 3–6 h. The cumulative excretion rate (Xu0→24) in urine was 26.43 ± 0.08% and 37.58 ± 0.11% for R-(−)- and S-(+)-triticonazole, respectively, indicating high enantioselectivity (P < 0.001). The cumulative excretion rate (Xu0→72) in faeces was 6.93 ± 0.03% and 6.77 ± 0.03% for R-(−)- and S-(+)-triticonazole, respectively, without a difference. The results showed that the total cumulative percentage of triticonazole enantiomers accounted for in urine and faeces was 64.00 ± 0.13% and 13.70 ± 0.32%, the urinary excretion of R-(−)- and S-(+)-triticonazole were significantly different and S-(+)-triticonazole was preferentially excreted. However, the faecal excretion of the enantiomers showed no difference.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"3 1","pages":"175 - 183"},"PeriodicalIF":0.0,"publicationDate":"2020-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75624374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-03DOI: 10.1080/03601234.2019.1684747
Haochang Su, Xiao-juan Hu, Linglong Wang, Wujie Xu, Yu Xu, Guo-liang Wen, Zhuo-jia Li, Yucheng Cao
Abstract Although the prevalence and concentrations of antibiotic resistance genes (ARGs) in aquaculture is receiving increasing scientific interest, there is little understanding of the direct sources and dissemination pathways of ARGs in marine aquaculture-reared organisms. This study investigated the dynamics of ARGs and the bacterial community throughout the rearing period in a typical marine aquaculture farm in South China. The results demonstrated that sul1 and qnrD were predominant in the sediment, and qnrD and qnrA were predominant in the intestinal tracts of shrimps. Network analysis showed that the chemical oxygen demand, total organic carbon, dissolved organic carbon, suspended solids, and total phosphorus were positively correlated with the predominant ARGs. The results of the network and source tracking analyses indicate that environmental factors and the bacterial community may drive the dissemination of ARGs dissemination in the environment and in shrimp reared by marine aquaculture, and sediment is the most direct and important medium in this dissemination. These results aid in improving our understanding of the sources, level, and dissemination of ARGs in marine aquaculture.
{"title":"Contamination of antibiotic resistance genes (ARGs) in a typical marine aquaculture farm: source tracking of ARGs in reared aquatic organisms","authors":"Haochang Su, Xiao-juan Hu, Linglong Wang, Wujie Xu, Yu Xu, Guo-liang Wen, Zhuo-jia Li, Yucheng Cao","doi":"10.1080/03601234.2019.1684747","DOIUrl":"https://doi.org/10.1080/03601234.2019.1684747","url":null,"abstract":"Abstract Although the prevalence and concentrations of antibiotic resistance genes (ARGs) in aquaculture is receiving increasing scientific interest, there is little understanding of the direct sources and dissemination pathways of ARGs in marine aquaculture-reared organisms. This study investigated the dynamics of ARGs and the bacterial community throughout the rearing period in a typical marine aquaculture farm in South China. The results demonstrated that sul1 and qnrD were predominant in the sediment, and qnrD and qnrA were predominant in the intestinal tracts of shrimps. Network analysis showed that the chemical oxygen demand, total organic carbon, dissolved organic carbon, suspended solids, and total phosphorus were positively correlated with the predominant ARGs. The results of the network and source tracking analyses indicate that environmental factors and the bacterial community may drive the dissemination of ARGs dissemination in the environment and in shrimp reared by marine aquaculture, and sediment is the most direct and important medium in this dissemination. These results aid in improving our understanding of the sources, level, and dissemination of ARGs in marine aquaculture.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"13 1","pages":"220 - 229"},"PeriodicalIF":0.0,"publicationDate":"2020-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78634836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-01DOI: 10.1080/03601234.2019.1673613
M. Požgajová, A. Navrátilová, J. Árvay, H. Ďúranová, A. Trakovická
Abstract Toxicity of heavy metals to living organisms is a worldwide research topic. Although, much has been discovered about cadmium and nickel impact on biological systems, a lot still remains unclear. We used inductively coupled plasma – optical emission spectroscopy to address the question of the effect of two different heavy metals nickel, and cadmium on intracellular ion balance. Increase or decrease of the content of several essential cations including Ca2+, Na+, K+, Mg2+, Cu2+, Fe3+ in the yeast Schizosaccharomyces pombe was determined. Our results revealed that the cell exposure to high nickel and cadmium concentrations led to significant elevation of Ca2+, Na+, Mg2+, Cu2+, Fe3+ levels in the yeast cell, while the content of K+ decreased. Correlation analyses showing in the presence of nickel and cadmium strong positive correlation among each tested element (Ca2+, Na+, Cu2+, Mg2+ and Fe3+) except for K+, demonstrate the significant impact of heavy metal treatment to ion homeostasis of the cell. Our data indicate that acute nickel and cadmium contamination leads to substantial ionome misbalance in yeast.
{"title":"Impact of cadmium and nickel on ion homeostasis in the yeast Schizosaccharomyces pombe","authors":"M. Požgajová, A. Navrátilová, J. Árvay, H. Ďúranová, A. Trakovická","doi":"10.1080/03601234.2019.1673613","DOIUrl":"https://doi.org/10.1080/03601234.2019.1673613","url":null,"abstract":"Abstract Toxicity of heavy metals to living organisms is a worldwide research topic. Although, much has been discovered about cadmium and nickel impact on biological systems, a lot still remains unclear. We used inductively coupled plasma – optical emission spectroscopy to address the question of the effect of two different heavy metals nickel, and cadmium on intracellular ion balance. Increase or decrease of the content of several essential cations including Ca2+, Na+, K+, Mg2+, Cu2+, Fe3+ in the yeast Schizosaccharomyces pombe was determined. Our results revealed that the cell exposure to high nickel and cadmium concentrations led to significant elevation of Ca2+, Na+, Mg2+, Cu2+, Fe3+ levels in the yeast cell, while the content of K+ decreased. Correlation analyses showing in the presence of nickel and cadmium strong positive correlation among each tested element (Ca2+, Na+, Cu2+, Mg2+ and Fe3+) except for K+, demonstrate the significant impact of heavy metal treatment to ion homeostasis of the cell. Our data indicate that acute nickel and cadmium contamination leads to substantial ionome misbalance in yeast.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"29 1","pages":"166 - 173"},"PeriodicalIF":0.0,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84447096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-01DOI: 10.1080/03601234.2019.1673612
F. Granados-Chinchilla, M. Arias-Andres, María Laura Fernández Montes de Oca, C. Rodríguez
Abstract Monensin (MON) is a coccidiostat used as a growth promoter that can reach the environment through fertilization with manure from farm animals. To verify whether field-relevant concentrations of this drug negatively influence the structure and activity of tropical soil bacteria, plate counts, CO2 efflux measurements, phospholipid fatty acids (PLFA) and community-level physiological profiling (CLPP) profiles were obtained for soil microcosms exposed to 1 or 10 mg kg−1 of MON across 11 days. Although 53% (1 mg kg−1) to 40% (10 mg kg−1) of the MON concentrations added to the microcosms dissipated within 5 days, a subtle concentration-dependent decrease in the number of culturable bacteria (<1 log CFU g−1), reduced (−20 to −30%) or exacerbated (+25%) soil CO2 effluxes, a marked shift of non-bacterial fatty acids, and altered respiration of amines (1.22-fold decrease) and polymers (1.70-fold increase) were noted in some of the treatments. These results suggest that MON quickly killed some microorganisms and that the surviving populations were selected and metabolically stimulated. Consequently, MON should be monitored in agronomic and environmental systems as part of One Health efforts.
摘要莫能菌素是一种生长促进剂，可通过动物粪便的施肥进入环境。为了验证该药物的田间相关浓度是否会对热带土壤细菌的结构和活性产生负面影响，研究人员对暴露于1或10 mg kg - 1 MON的土壤微生物进行了11天的平板计数、二氧化碳外排测量、磷脂脂肪酸(PLFA)和社区水平生理谱(CLPP)谱分析。虽然添加到微环境中的MON浓度在5天内消散了53% (1 mg kg - 1)至40% (10 mg kg - 1)，但在某些处理中，可培养细菌数量(<1 log CFU g - 1)出现了细微的浓度依赖性减少，土壤CO2外流量减少(- 20至- 30%)或加剧(+25%)，非细菌脂肪酸发生了显著变化，胺类呼吸(减少1.22倍)和聚合物(增加1.70倍)发生了改变。这些结果表明，单胞菌能迅速杀死一些微生物，幸存的种群被选择和代谢刺激。因此，应在农艺和环境系统中监测MON，作为“同一个健康”努力的一部分。
{"title":"Effect of the veterinary ionophore monensin on the structure and activity of a tropical soil bacterial community","authors":"F. Granados-Chinchilla, M. Arias-Andres, María Laura Fernández Montes de Oca, C. Rodríguez","doi":"10.1080/03601234.2019.1673612","DOIUrl":"https://doi.org/10.1080/03601234.2019.1673612","url":null,"abstract":"Abstract Monensin (MON) is a coccidiostat used as a growth promoter that can reach the environment through fertilization with manure from farm animals. To verify whether field-relevant concentrations of this drug negatively influence the structure and activity of tropical soil bacteria, plate counts, CO2 efflux measurements, phospholipid fatty acids (PLFA) and community-level physiological profiling (CLPP) profiles were obtained for soil microcosms exposed to 1 or 10 mg kg−1 of MON across 11 days. Although 53% (1 mg kg−1) to 40% (10 mg kg−1) of the MON concentrations added to the microcosms dissipated within 5 days, a subtle concentration-dependent decrease in the number of culturable bacteria (<1 log CFU g−1), reduced (−20 to −30%) or exacerbated (+25%) soil CO2 effluxes, a marked shift of non-bacterial fatty acids, and altered respiration of amines (1.22-fold decrease) and polymers (1.70-fold increase) were noted in some of the treatments. These results suggest that MON quickly killed some microorganisms and that the surviving populations were selected and metabolically stimulated. Consequently, MON should be monitored in agronomic and environmental systems as part of One Health efforts.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"10 1","pages":"127 - 134"},"PeriodicalIF":0.0,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85448882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-01DOI: 10.1080/03601234.2019.1674103
V. Balderas-Hernández, J. T. Ornelas-Salas, A. P. Barba de la Rosa, A. De León-Rodríguez
Abstract The presence of diethyl-phthalate (DEP), dibutyl-phthalate (DBP), butylbenzyl-phthalate (BBP), diethylhexyl-phthalate (DEHP) and diisononyl-phthalate (DINP) was determined in 295 tequila samples. They were grouped by age of maturation (white, aged, extra aged or ultra aged) and year of production (between 2013 and 2018). Gas Chromatography coupled with Mass Spectrometry was used for identification and quantification. The results showed that 65 samples (22% of the total) were phthalate free. DEP (0.13-0.27 mg/kg), BBP (0.05–2.91 mg/kg) and DINP (1.64–3.43 mg/kg) were detected in 11 (3.73%), 37 (12.54%) and 5 (1.69%) samples, respectively. But, these concentrations did not exceed the maximum permitted limits (MPL) of phthalates for alcoholic beverages. DBP (0.01–2.20 mg/kg) and DEHP (0.03–4.64 mg/kg) were detected in 96 (32.54%) and 224 (75.93%) samples, from them only 10 (3.39%) and 15 (5.08%) samples, respectively, exceeded the MPL for alcoholic beverages and they were few tequilas produced in the year 2014 or before. DEHP was the most frequent phthalate found in tequila and observed DEHP concentrations were 2-times higher in ultra aged tequilas compared to those in white tequilas. We concluded that all tequilas produced in 2015 and after, satisfied the international standards for these compounds.
{"title":"Diminution of migration of phthalic acid esters in tequila beverage by the year of production","authors":"V. Balderas-Hernández, J. T. Ornelas-Salas, A. P. Barba de la Rosa, A. De León-Rodríguez","doi":"10.1080/03601234.2019.1674103","DOIUrl":"https://doi.org/10.1080/03601234.2019.1674103","url":null,"abstract":"Abstract The presence of diethyl-phthalate (DEP), dibutyl-phthalate (DBP), butylbenzyl-phthalate (BBP), diethylhexyl-phthalate (DEHP) and diisononyl-phthalate (DINP) was determined in 295 tequila samples. They were grouped by age of maturation (white, aged, extra aged or ultra aged) and year of production (between 2013 and 2018). Gas Chromatography coupled with Mass Spectrometry was used for identification and quantification. The results showed that 65 samples (22% of the total) were phthalate free. DEP (0.13-0.27 mg/kg), BBP (0.05–2.91 mg/kg) and DINP (1.64–3.43 mg/kg) were detected in 11 (3.73%), 37 (12.54%) and 5 (1.69%) samples, respectively. But, these concentrations did not exceed the maximum permitted limits (MPL) of phthalates for alcoholic beverages. DBP (0.01–2.20 mg/kg) and DEHP (0.03–4.64 mg/kg) were detected in 96 (32.54%) and 224 (75.93%) samples, from them only 10 (3.39%) and 15 (5.08%) samples, respectively, exceeded the MPL for alcoholic beverages and they were few tequilas produced in the year 2014 or before. DEHP was the most frequent phthalate found in tequila and observed DEHP concentrations were 2-times higher in ultra aged tequilas compared to those in white tequilas. We concluded that all tequilas produced in 2015 and after, satisfied the international standards for these compounds.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"56 1","pages":"148 - 154"},"PeriodicalIF":0.0,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84390548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-01DOI: 10.1080/03601234.2019.1665408
Yuqing Shen, Jianyun Zhang, Jingqian Xie, Jing Liu
Abstract Information regarding the enantioselective endocrine disruption of chiral herbicides is scarce. This study assessed the disrupting effects of eight typical chiral herbicides on corticosteroids (including glucocorticoids and mineralocorticoids). Enantioselectivity of eight chiral herbicides were evaluated for their agonistic/antagonistic effects on glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) with CHOK1 cell line using reporter gene assay. Their influence on the production of corticosteroids were further investigated in H295R cell line using enzyme-linked immunosorbent assay (ELISA). None of the racemates or enantiomers of eight chiral herbicides exhibited GR or MR agonistic activity at non-cytotoxic concentrations. However, rac-propisochlor and S-imazamox antagonized cortisol-induced transactivation of GR by 21.79% and 38.73% at the concentration of 1.0 × 10−7 M and 1.0 × 10−6 M, respectively, and R-napropamide remarkably attenuated aldosterone-induced MR transactivation by 68.78% at 1.0 × 10−6 M. The secretion of cortisol was significantly restrained after treated with 1.0 × 10−6 M rac-propisochlor and rac-/R-napropamide at the concentration of 1.0 × 10−6 M by 26.49%, 30.10% and 35.27%, respectively, while this glucocorticoid was remarkably induced by 1.0 × 10−5 M rac-diclofop-methyl and its two enantiomers at the concentration of 1.0 × 10−5 M by 75.60%, 100.1% and 68.78%, respectively. Exposure to rac-propisochlor (1.0 × 10−6 M), S-diclofop-methyl (1.0 × 10−5 M) or rac-/S-/R- acetochlor (1.0 × 10−6 M) and rac-/S-/R-lactofen (1.0 × 10−6 M) inhibited the secretion of aldosterone by approximately 40%. Our findings suggested that chiral herbicides disrupted corticosteroid homeostasis in an enantioselective way. Therefore, more comprehensive screening is required to better understand the ecological and health risks of chiral pesticides.
{"title":"In vitro assessment of corticosteroid effects of eight chiral herbicides","authors":"Yuqing Shen, Jianyun Zhang, Jingqian Xie, Jing Liu","doi":"10.1080/03601234.2019.1665408","DOIUrl":"https://doi.org/10.1080/03601234.2019.1665408","url":null,"abstract":"Abstract Information regarding the enantioselective endocrine disruption of chiral herbicides is scarce. This study assessed the disrupting effects of eight typical chiral herbicides on corticosteroids (including glucocorticoids and mineralocorticoids). Enantioselectivity of eight chiral herbicides were evaluated for their agonistic/antagonistic effects on glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) with CHOK1 cell line using reporter gene assay. Their influence on the production of corticosteroids were further investigated in H295R cell line using enzyme-linked immunosorbent assay (ELISA). None of the racemates or enantiomers of eight chiral herbicides exhibited GR or MR agonistic activity at non-cytotoxic concentrations. However, rac-propisochlor and S-imazamox antagonized cortisol-induced transactivation of GR by 21.79% and 38.73% at the concentration of 1.0 × 10−7 M and 1.0 × 10−6 M, respectively, and R-napropamide remarkably attenuated aldosterone-induced MR transactivation by 68.78% at 1.0 × 10−6 M. The secretion of cortisol was significantly restrained after treated with 1.0 × 10−6 M rac-propisochlor and rac-/R-napropamide at the concentration of 1.0 × 10−6 M by 26.49%, 30.10% and 35.27%, respectively, while this glucocorticoid was remarkably induced by 1.0 × 10−5 M rac-diclofop-methyl and its two enantiomers at the concentration of 1.0 × 10−5 M by 75.60%, 100.1% and 68.78%, respectively. Exposure to rac-propisochlor (1.0 × 10−6 M), S-diclofop-methyl (1.0 × 10−5 M) or rac-/S-/R- acetochlor (1.0 × 10−6 M) and rac-/S-/R-lactofen (1.0 × 10−6 M) inhibited the secretion of aldosterone by approximately 40%. Our findings suggested that chiral herbicides disrupted corticosteroid homeostasis in an enantioselective way. Therefore, more comprehensive screening is required to better understand the ecological and health risks of chiral pesticides.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"44 1","pages":"102 - 91"},"PeriodicalIF":0.0,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81587918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-01DOI: 10.1080/03601234.2019.1667190
John Kasumba, Keerthi Appala, G. Agga, John H. Loughrin, E. Conte
Abstract We investigated the anaerobic degradation of tetracycline antibiotics (tetracycline [TC], oxytetracycline [OTC] and chlortetracycline [CTC]) in swine, cattle, and poultry manures. The manures were anaerobically digested inside polyvinyl chloride batch reactors for 64 days at room temperature. The degradation rate constants and half-lives of the parent tetracyclines were determined following first-order kinetics. For CTC the fastest degradation rate was observed in swine manure (k = 0.016 ± 0.001 d−1; half-life = 42.8 days), while the slowest degradation rate was observed in poultry litter (k = 0.0043 ± 0.001 d−1; half-life = 161 days). The half-lives of OTC ranged between 88.9 (cattle manure) and 99.0 days (poultry litter), while TC persisted the longest of the tetracycline antibiotics studied with half-lives ranging from 92.4 days (cattle manure) to 330 days (swine manure). In general, the tetracyclines were found to degrade faster in cattle manure, which had the lowest concentrations of organic matter and metals as compared to swine and poultry manures. Our results demonstrate that tetracycline antibiotics persist in the animal manure after anaerobic digestion, which can potentially lead to emergence and persistence of antibiotic resistant bacteria in the environment when anaerobic digestion byproducts are land applied for crop production.
{"title":"Anaerobic digestion of livestock and poultry manures spiked with tetracycline antibiotics","authors":"John Kasumba, Keerthi Appala, G. Agga, John H. Loughrin, E. Conte","doi":"10.1080/03601234.2019.1667190","DOIUrl":"https://doi.org/10.1080/03601234.2019.1667190","url":null,"abstract":"Abstract We investigated the anaerobic degradation of tetracycline antibiotics (tetracycline [TC], oxytetracycline [OTC] and chlortetracycline [CTC]) in swine, cattle, and poultry manures. The manures were anaerobically digested inside polyvinyl chloride batch reactors for 64 days at room temperature. The degradation rate constants and half-lives of the parent tetracyclines were determined following first-order kinetics. For CTC the fastest degradation rate was observed in swine manure (k = 0.016 ± 0.001 d−1; half-life = 42.8 days), while the slowest degradation rate was observed in poultry litter (k = 0.0043 ± 0.001 d−1; half-life = 161 days). The half-lives of OTC ranged between 88.9 (cattle manure) and 99.0 days (poultry litter), while TC persisted the longest of the tetracycline antibiotics studied with half-lives ranging from 92.4 days (cattle manure) to 330 days (swine manure). In general, the tetracyclines were found to degrade faster in cattle manure, which had the lowest concentrations of organic matter and metals as compared to swine and poultry manures. Our results demonstrate that tetracycline antibiotics persist in the animal manure after anaerobic digestion, which can potentially lead to emergence and persistence of antibiotic resistant bacteria in the environment when anaerobic digestion byproducts are land applied for crop production.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"1 1","pages":"135 - 147"},"PeriodicalIF":0.0,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89909534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-01DOI: 10.1080/03601234.2019.1674593
Q. Binh, D. Tungtakanpoung, P. Kajitvichyanukul
Abstract In this study, the similarities and differences of the adsorption mechanisms between dichlorvos and pymetrozine and coconut fiber biowaste sorbent (CF-BWS) were investigated. CF-BWS was produced using the slow pyrolysis process at 600 °C for 4 h. HCl acid modification was used to improve the specific surface area. The properties of CF-BWS were analyzed by SEM, FT-IR, BET, and pHpzc. The adsorption kinetics of dichlorvos and pymetrozine on the CF-BWS were well explained by the pseudo-second-order model. The adsorption isotherms for both insecticides were followed the Langmuir isotherm. The difference in molecular structures and surface chemistry caused the difference in adsorption mechanisms of both insecticides. The pore-filling and the hydrophobic interactions were the key mechanisms for both insecticide adsorptions. However, the π–π electron donor–acceptor interaction played the major role in the pymetrozine adsorption but hardly impacted on the adsorption of dichlorvos. The hydrogen bonding mechanism was pronounced in the pymetrozine adsorption, but it had little influence on the dichlorvos adsorption. The CF-BWS is exhibited as an excellent material for the removal of both pollutants and has high potential to be used further as the adsorbent in water treatment process.
{"title":"Similarities and differences in adsorption mechanism of dichlorvos and pymetrozine insecticides with coconut fiber biowaste sorbent","authors":"Q. Binh, D. Tungtakanpoung, P. Kajitvichyanukul","doi":"10.1080/03601234.2019.1674593","DOIUrl":"https://doi.org/10.1080/03601234.2019.1674593","url":null,"abstract":"Abstract In this study, the similarities and differences of the adsorption mechanisms between dichlorvos and pymetrozine and coconut fiber biowaste sorbent (CF-BWS) were investigated. CF-BWS was produced using the slow pyrolysis process at 600 °C for 4 h. HCl acid modification was used to improve the specific surface area. The properties of CF-BWS were analyzed by SEM, FT-IR, BET, and pHpzc. The adsorption kinetics of dichlorvos and pymetrozine on the CF-BWS were well explained by the pseudo-second-order model. The adsorption isotherms for both insecticides were followed the Langmuir isotherm. The difference in molecular structures and surface chemistry caused the difference in adsorption mechanisms of both insecticides. The pore-filling and the hydrophobic interactions were the key mechanisms for both insecticide adsorptions. However, the π–π electron donor–acceptor interaction played the major role in the pymetrozine adsorption but hardly impacted on the adsorption of dichlorvos. The hydrogen bonding mechanism was pronounced in the pymetrozine adsorption, but it had little influence on the dichlorvos adsorption. The CF-BWS is exhibited as an excellent material for the removal of both pollutants and has high potential to be used further as the adsorbent in water treatment process.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"121 1","pages":"103 - 114"},"PeriodicalIF":0.0,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89047510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-02DOI: 10.1080/03601234.2019.1655376
E. C. Martins, V. Melo, G. Abate
Abstract The sorption and desorption of diuron by soil samples from Horizons A and B (HA and HB) and by their different clay fractions were investigated, using two soil samples, classified as Typic Argiudoll and Oxic Argiudoll. The sorption and desorption curves were adjusted to the Freundlich model and evaluated by parameters Kf, Kd and Koc. Based on the data of groundwater ubiquity score (GUS), leachability index (LIX) and hysteresis index (HI), the risk of groundwater pollution was evaluated. The Kd values obtained for soil samples were between 4.5 mL g−1 (Oxic Argiudoll – HB) and 15.9 mL g−1 (Typic Argiudoll – HA) and between 1.13 and 14.0 mL g−1 for the different mineral fractions, whereas the Koc values varied between 276 (Oxic Argiudoll – HB) and 462 (Typic Argiudoll – HA). According to the parameter GUS, only Oxic Argiudoll – HB presented leaching potential, and based on the LIX index this same soil presented the highest leaching potential. Some samples presented low LIX and GUS values, indicating no leaching potential, but none presented HI results indicative of hysteresis, suggesting weak bonds between diuron and the soil samples and, hence, the risk of groundwater pollution by diuron.
以典型阿吉多尔土和氧性阿吉多尔土为研究对象，研究了地平线A和地平线B土壤样品(HA和HB)及其不同粘土组分对迪乌隆的吸附和解吸作用。将吸附和解吸曲线调整为Freundlich模型，并通过参数Kf、Kd和Koc进行评价。基于地下水泛在性评分(GUS)、可浸性指数(LIX)和滞后指数(HI)数据，对地下水污染风险进行了评价。土壤样品的Kd值在4.5 mL g−1(氧基Argiudoll - HB)和15.9 mL g−1(典型Argiudoll - HA)之间，不同矿物组分的Kd值在1.13 ~ 14.0 mL g−1之间，而Koc值在276(氧基Argiudoll - HB)和462(典型Argiudoll - HA)之间变化。根据GUS参数，只有氧基Argiudoll - HB具有浸出电位，而根据LIX指数，该土壤的浸出电位最高。一些样品的LIX和GUS值较低，表明没有淋出潜力，但没有一个样品的HI结果表明存在滞后，表明狄乌隆与土壤样品之间的结合较弱，因此存在狄乌隆污染地下水的风险。
{"title":"Sorption and desorption of diuron on Typic Argiudoll, Oxic Argiudoll and on their clay fractions: environmental aspects","authors":"E. C. Martins, V. Melo, G. Abate","doi":"10.1080/03601234.2019.1655376","DOIUrl":"https://doi.org/10.1080/03601234.2019.1655376","url":null,"abstract":"Abstract The sorption and desorption of diuron by soil samples from Horizons A and B (HA and HB) and by their different clay fractions were investigated, using two soil samples, classified as Typic Argiudoll and Oxic Argiudoll. The sorption and desorption curves were adjusted to the Freundlich model and evaluated by parameters Kf, Kd and Koc. Based on the data of groundwater ubiquity score (GUS), leachability index (LIX) and hysteresis index (HI), the risk of groundwater pollution was evaluated. The Kd values obtained for soil samples were between 4.5 mL g−1 (Oxic Argiudoll – HB) and 15.9 mL g−1 (Typic Argiudoll – HA) and between 1.13 and 14.0 mL g−1 for the different mineral fractions, whereas the Koc values varied between 276 (Oxic Argiudoll – HB) and 462 (Typic Argiudoll – HA). According to the parameter GUS, only Oxic Argiudoll – HB presented leaching potential, and based on the LIX index this same soil presented the highest leaching potential. Some samples presented low LIX and GUS values, indicating no leaching potential, but none presented HI results indicative of hysteresis, suggesting weak bonds between diuron and the soil samples and, hence, the risk of groundwater pollution by diuron.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"31 1","pages":"11 - 18"},"PeriodicalIF":0.0,"publicationDate":"2020-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89322364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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