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Comparison of the Accuracy and Efficiency of Two Dynamic Navigation System Workflow for Fiber-post Removal: Small versus Large Field-of-view Registration Workflows 比较两种动态导航系统工作流程在纤维桩移除方面的准确性和效率:小视野与大视野注册工作流程。
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.joen.2024.06.011

Introduction

This study investigates the feasibility of a dynamic navigation system (DNS) small field of view workflow (DNS-SFOVw) for fiber-post removal and compares its accuracy and efficiency to the conventional large field of view workflow (DNS-LFOVw).

Methods

Fifty-six extracted human maxillary molars were divided into DNS-SFOVw (n = 28) and DNS-LFOVw (n = 28). The palatal canal was restored with an intraradicular RelyX fiber post and luted with RelyX Unicem; a core buildup was used. Teeth were mounted in a 3D-printed surgical jaw. A preoperative cone-beam computed tomography (CBCT) scan was taken with a 40 × 40 mm FOV for the DNS-SFOVw and a single arch CBCT scan for the DNS-LFOVw. The drilling entry point, trajectory, angle, and depth were planned in the X-guide software. The DNS registration method for the DNS-SFOVw was virtual-based registration on teeth, and the marker point-based method was used for the DNS-LFOVw. The fiber posts were drilled out under DNS guidance. A postoperative CBCT scan was taken. Three-dimensional deviations, angular deflection, number of mishaps, registration, and total operation time were calculated.

Results

The DNS-SFOVw was as accurate as DNS-LFOVw (P > .05). The DNS-LFOVw registration time was less than DNS-SFOVw (P < .05). There was no difference in the number of mishaps (P > .05). Both DNS-SFOVw and DNS-LFOVw were time-efficient, with DNS-LFOVw taking less total operational time (P < .05).

Conclusion

Within the limitations of this in-vitro study, the DNS-SFOVw was as accurate as the DNS-LFOVw for fiberpost removal. Both DNS-LFOVw and DNS-SFOVw were time-efficient in removing fiber-posts.
导言:本研究探讨了动态导航系统(DNS)小视野工作流程(DNS-SFOVw)用于纤维桩拔除的可行性,并将其准确性和效率与传统的大视野工作流程(DNS-LFOVw)进行了比较:56颗拔出的人类上颌磨牙被分为DNS-SFOVw(28颗)和DNS-LFOVw(28颗)。用 RelyX 纤维桩修复腭管,并用 RelyX Unicem 树脂灌注;使用核心堆积。牙齿安装在 3D 打印的手术颌中。术前进行锥束计算机断层扫描(CBCT),DNS-SFOVw 的扫描范围为 40 x 40 mm,DNS-LFOVw 的扫描范围为单弓 CBCT。钻孔的切入点、轨迹、角度和深度均在 X-guide 软件中进行了规划。DNS-SFOVw 的 DNS 套准方法是基于牙齿的虚拟套准,而 DNS-LFOVw 则是基于标记点的方法。纤维桩是在 DNS 引导下钻出的。术后进行 CBCT 扫描。计算了三维(3D)偏差、角度偏差、失误次数、注册和总手术时间:结果:DNS-SFOVw 与 DNS-LFOVw 一样精确(P>.05)。DNS-LFOVw 登记时间少于 DNS-SFOVw(P.05)。DNS-SFOVw 和 DNS-LFOVw 都很省时,DNS-LFOVw 的总操作时间更短(p 结论:在这项体外研究的限制条件下,DNS-SFOVw 与 DNS-LFOVw 在纤维桩移除方面的精确度相当。DNS-LFOVw 和 DNS-SFOVw 在清除纤维桩方面都很省时。
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引用次数: 0
Use of a Penrose Drain for Decompression of a Large Periapical Lesion: A Case Report With 4.5-Year Follow-up 使用 Penrose 引流管为大面积根尖周病变减压:随访 4.5 年的病例报告
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.joen.2024.07.005
Feng-Ming Wang DDS, PhD , Hui Liang BDS, MS, PhD , Gerald N. Glickman DDS, MS, MBA, JD , James L. Gutmann DDS, PhD, FACD, FICD, FADI, FAHD, IBE, FDSRCSEd
Managing large cyst-like periapical lesions poses significant challenges, especially when nonsurgical treatment or retreatment options are ineffective. Despite its efficacy, decompression remains an underutilized minimally invasive alternative in modern dentistry. This case report describes the use of a Penrose drain for decompression following aspiration and irrigation to manage a large periapical lesion associated with a 56-year-old man's maxillary lateral incisor. The lesion had caused thinning and perforation of the facial and palatal cortical plates, as well as the inferior border of the nasal fossa. Cone-beam computed tomography was used to evaluate the lesion preoperatively and to assess the healing progress postoperatively. Complete 3-dimensional healing was observed after a subsequent root-end surgery performed 3.5 years post-decompression. This report suggests that decompression using a Penrose drain in combination with aspiration and irrigation could be a simple but effective modality for managing large cyst-like periapical lesions when nonsurgical endodontics are attempted and deemed ineffective.
治疗大型根尖囊肿样病变是一项巨大的挑战,尤其是在非手术治疗或再治疗方案无效的情况下。尽管减压术效果显著,但在现代牙科中仍是一种未得到充分利用的微创替代方法。本病例报告描述了在对一名 56 岁男性的上颌侧切牙进行抽吸和冲洗后,使用 Penrose 引流管进行减压,以处理与该患者上颌侧切牙相关的巨大根尖周病变。该病变导致面部和腭部皮质板以及鼻窝下缘变薄和穿孔。锥形束计算机断层扫描用于术前评估病变情况和术后评估愈合进度。在减压术后 3.5 年再次进行根端手术后,观察到了完全的三维愈合。该报告表明,在尝试非手术根管治疗无效的情况下,使用彭罗斯引流管减压并结合抽吸和冲洗,是治疗大型根尖周囊肿样病变的一种简单而有效的方法。
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引用次数: 0
Effect of the Time Interval of Functional Loading on the Periapical Healing of Endodontically Treated Mandibular Molar Teeth with Pulp Necrosis and Asymptomatic Apical Periodontitis: A Split-mouth Randomized Clinical Study 功能负荷时间间隔对牙髓坏死和无症状根尖牙周炎下颌磨牙根管治疗后根尖周愈合的影响:分口随机临床研究。
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.joen.2024.07.013
Gunadhar Singh Kangjam MDS , Ajay Logani MDS , Veena Jain MDS , Vijay Kumar MDS , Amrita Chawla MDS , Babina Chirom MDS

Introduction

This prospective study evaluated the effect of immediate functional loading (IFL) of a full-coverage prosthesis on the clinical and radiographic outcome of nonsurgical endodontic therapy (NSET) performed on mandibular molar teeth with pulp necrosis and asymptomatic apical periodontitis.

Methods

In 20 subjects, standardized 2-visit NSET was performed in bilateral mandibular first molar teeth (split-mouth model) with a diagnosis of pulp necrosis and asymptomatic apical periodontitis exhibiting a radiographic periapical index (PAI) score ≥3. In each subject, the nonvital bilateral mandibular first molar teeth were randomized to 1 of 2 groups: the IFL group or the functional loading after a time interval of 6 months group. Provisional full-coverage prostheses were provided within 7 days after the completion of endodontic therapy. The cases were followed up clinically and radiographically at 6 and 12 months. Radiographs were assessed for periapical healing based on PAI scores, which were dichotomized as healed (PAI score ≤2) or nonhealed (PAI score ≥3). The data were compared using chi-square and Fisher exact tests.

Results

A recall rate of 100% was achieved at the end of 12 months. All teeth in the IFL group and the functional loading after a time interval of 6 months group were clinically asymptomatic. According to an intergroup comparison between the proportion of teeth healed and nonhealed (radiographic), there was a significant difference (P < .05).

Conclusions

IFL of a full-coverage prosthesis in endodontically treated mandibular molar teeth with pulp necrosis and asymptomatic apical periodontitis delayed periapical healing.
导言:这项前瞻性研究评估了对牙髓坏死和无症状根尖牙周炎的下颌第一磨牙进行非手术牙髓治疗(NSET)后,全覆盖修复体的即刻功能性加载(IFL)对临床和影像学结果的影响:在 20 名受试者中,对诊断为牙髓坏死和无症状根尖牙周炎的双侧下颌第一磨牙(分口模型)进行了两次标准化的非手术根管治疗(NSET),其放射根尖周指数(PAI)得分≥3。每名受试者的双侧下颌第一磨牙均无髓质,随机分为两组,即 IFL 组(即刻功能负荷)或 NFL 组(间隔 6 个月后功能负荷)。牙髓治疗完成后七天内提供临时全覆盖修复体。分别在 6 个月和 12 个月后对病例进行临床和影像学随访。根据 PAI 评分评估根尖周愈合情况,并将其分为愈合(PAI 评分≤2)和未愈合(PAI 评分≥3)两种。数据比较采用卡方检验(Chi-square)和费舍尔精确检验(Fischer's exact tests):结果:12 个月后的复诊率为 100%。IFL 组和 NFL 组的所有牙齿均无临床症状。在组间比较中,痊愈和未痊愈的牙齿比例(影像学)有显著差异(p):对牙髓坏死和无症状根尖牙周炎的下颌磨牙进行根管治疗后,全覆盖修复体的即刻功能性加载延迟了根尖周愈合。
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引用次数: 0
Comparison of Accuracy and Operation Time in Robotic, Dynamic, and Static-Assisted Endodontic Microsurgery: An In Vitro Study 机器人、动态和静态辅助根管显微手术的精确度和操作时间比较:体外研究
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.joen.2024.05.018

Introduction

This study aimed to compare the accuracy and operation time (OT) of robotic-assisted endodontic microsurgery (RA-EMS), dynamic navigation-guided (DN-guided) EMS, and static navigation-guided (SN-guided) EMS.

Methods

Seventy-two teeth from three sets of standardized jaw models (TrueTooth, DELendo, Santa Barbara, CA) randomly assigned into 3 groups underwent osteotomy and root-end resection. Preoperative plans and postoperative cone-beam computed tomography images were imported into an accuracy analysis system and aligned based on the anatomical structures to assess accuracy. The OT was recorded from the moment the foot pedal was pressed down until the bur reached the target depth. Statistical analyses were conducted using Kruskal–Wallis and Scheirer–Ray–Hare tests, with significance set at P < .05.

Results

RA-EMS exhibited significantly higher accuracy than DN- and SN-guided EMS in terms of platform, angular, and resection angular deviations (P < .05). Additionally, RA-EMS exhibited significantly higher accuracy than DN-guided EMS in resection length deviation (P < .05). Significant differences were also observed in OTs between the 3 approaches, with SN-guided EMS showing the shortest OT, followed by RA-EMS and DN-guided EMS. Differences in jaw types within the DN-guided EMS group were observed in terms of angular deviation (P < .05).

Conclusions

All 3 treatment approaches demonstrated acceptable clinical accuracy and OT. RA-EMS exhibited superior accuracy, suggesting its potential application prospects in endodontics. Further high-quality clinical studies are warranted.
导言:本研究旨在比较机器人辅助牙髓显微手术(RA-EMS)、动态导航引导(DN-guided)EMS和静态导航引导(SN-guided)EMS的准确性和手术时间(OT):从三套标准化颌骨模型(TrueTooth, DELendo, Santa Barbara, CA)中随机分配出 72 颗牙齿,分成三组进行截骨术和根端切除术。术前计划和术后锥束计算机断层扫描图像被导入精确度分析系统,并根据解剖结构进行对齐以评估精确度。从踩下脚踏板开始记录 OT,直到钻针到达目标深度。统计分析采用 Kruskal-Wallis 和 Scheirer-Ray-Hare 检验,显著性设定为 p <0.05:在平台偏差、角度偏差和切除角度偏差方面,RA-EMS 的准确性明显高于 DN 和 SN 引导的 EMS(p < 0.05)。此外,在切除长度偏差方面,RA-EMS 的准确性明显高于 DN 引导的 EMS(P < 0.05)。三种方法的OT也存在显著差异,SN引导的EMS显示出最短的OT,其次是RA-EMS和DN引导的EMS。在角度偏差方面,DN引导的EMS组的颌骨类型也存在差异(P < 0.05):所有三种治疗方法都表现出了可接受的临床准确性和 OT。结论:三种治疗方法都表现出了可接受的临床准确性和OT,RA-EMS表现出了更高的准确性,这表明它在牙髓病学中具有潜在的应用前景。有必要进一步开展高质量的临床研究。
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引用次数: 0
Effect of Novel and Traditional Intracanal Medicaments on Biofilm Viability and Composition 新型和传统龈内药物对生物膜活力和组成的影响
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.joen.2024.07.003
Shuk Yi Siu BDS , Akhila Pudipeddi BSc, MSc , Vijetha Vishwanath MDS , Angeline Hui Cheng Lee MDS , Amelia Wan Tin Cheung MDS , Gary Shun Pan Cheung MDS, PhD , Prasanna Neelakantan MDS, PhD

Introduction

The aim of this study was to test the hypothesis that a combination of D-amino acids (DAAs) and trans-cinnamaldehyde (TC) demonstrates superior antibiofilm activity to calcium hydroxide (CH) and untreated controls.

Methods

In this 3-part in vitro study, the concentration of DAAs (D-methionine, D-leucine, D-tyrosine, and D-tryptophan) that would significantly decrease Enterococcus faecalis and Actinomyces naeslundii biofilm biomass was first determined. Then, the effect of TC + selected DAAs on polymicrobial biofilms was characterized by quantifying the biomass and biofilm viability. Finally, the antibiofilm effects of TC + DAA was compared with CH and untreated controls by (i) determining bacterial viability and (ii) quantifying biofilm matrix composition using selective fluorescence-binding analysis. Statistical analysis was performed using one-way ANOVA and appropriate multiple comparisons test, with P < .05 considered as statistically significant.

Results

TC (0.06%) + D-tyrosine (1 mM) + D-tryptophan (25 mM) significantly reduced the biomass and biofilm viability compared to the control (P < .05). While no significant difference was observed between TC + DAA and CH in the cultivable bacterial counts (P > .05), confocal microscopy demonstrated a significantly greater percentage of dead bacteria in TC + DAA-treated biofilms compared to CH and the control (P < .05). TC + DAA significantly decreased the biovolume and all the examined components of the biofilm matrix quantity compared to the control, while CH significantly reduced only the exopolysaccharide quantity (P < .05).

Conclusion

The combination of TC + D-tyrosine + D-tryptophan demonstrated superior antibiofilm activity (biofilm bacterial killing and reduction of matrix quantity) to CH and has potential to be developed as an intracanal medicament.
简介:本研究旨在验证一种假设,即 D-氨基酸(DAA)和反式肉桂醛(TC)的组合比氢氧化钙(CH)和未处理的对照组具有更强的抗生物膜活性:在这项由三部分组成的体外研究中,首先确定了能显著降低粪肠球菌和奈氏放线菌生物膜生物量的 DAA(D-蛋氨酸、D-亮氨酸、D-酪氨酸和 D-色氨酸)浓度。然后,通过量化生物量和生物膜活力,确定了 TC + 所选 DAAs 对多微生物生物膜的影响。最后,通过(i)确定细菌存活率和(ii)使用选择性荧光结合分析量化生物膜基质组成,将 TC+DAA 的抗生物膜效果与 CH 和未处理的对照组进行比较。统计分析采用单因素方差分析和适当的多重比较检验,结果如下:与对照组相比,TC(0.06%)+ D-酪氨酸(1 mM)+ D-色氨酸(25 mM)显著降低了生物量和生物膜活力(P.05),共聚焦显微镜显示,与 CH 和对照组相比,TC+DAA 处理的生物膜中死亡细菌的比例显著增加(PConclusion:TC + D-酪氨酸 + D-色氨酸的抗生物膜活性(杀灭生物膜细菌和减少基质数量)优于 CH,具有开发为龋内药物的潜力。
{"title":"Effect of Novel and Traditional Intracanal Medicaments on Biofilm Viability and Composition","authors":"Shuk Yi Siu BDS ,&nbsp;Akhila Pudipeddi BSc, MSc ,&nbsp;Vijetha Vishwanath MDS ,&nbsp;Angeline Hui Cheng Lee MDS ,&nbsp;Amelia Wan Tin Cheung MDS ,&nbsp;Gary Shun Pan Cheung MDS, PhD ,&nbsp;Prasanna Neelakantan MDS, PhD","doi":"10.1016/j.joen.2024.07.003","DOIUrl":"10.1016/j.joen.2024.07.003","url":null,"abstract":"<div><h3>Introduction</h3><div>The aim of this study was to test the hypothesis that a combination of D-amino acids (DAAs) and <em>trans</em>-cinnamaldehyde (TC) demonstrates superior antibiofilm activity to calcium hydroxide (CH) and untreated controls.</div></div><div><h3>Methods</h3><div>In this 3-part <em>in vitro</em> study, the concentration of DAAs (D-methionine, D-leucine, D-tyrosine, and D-tryptophan) that would significantly decrease <em>Enterococcus faecalis</em> and <em>Actinomyces naeslundii</em> biofilm biomass was first determined. Then, the effect of TC + selected DAAs on polymicrobial biofilms was characterized by quantifying the biomass and biofilm viability. Finally, the antibiofilm effects of TC + DAA was compared with CH and untreated controls by (i) determining bacterial viability and (ii) quantifying biofilm matrix composition using selective fluorescence-binding analysis. Statistical analysis was performed using one-way ANOVA and appropriate multiple comparisons test, with <em>P</em> &lt; .05 considered as statistically significant.</div></div><div><h3>Results</h3><div>TC (0.06%) + D-tyrosine (1 mM) + D-tryptophan (25 mM) significantly reduced the biomass and biofilm viability compared to the control (<em>P</em> &lt; .05). While no significant difference was observed between TC + DAA and CH in the cultivable bacterial counts (<em>P</em> &gt; .05), confocal microscopy demonstrated a significantly greater percentage of dead bacteria in TC + DAA-treated biofilms compared to CH and the control (<em>P</em> &lt; .05). TC + DAA significantly decreased the biovolume and all the examined components of the biofilm matrix quantity compared to the control, while CH significantly reduced only the exopolysaccharide quantity (<em>P</em> &lt; .05).</div></div><div><h3>Conclusion</h3><div>The combination of TC + D-tyrosine + D-tryptophan demonstrated superior antibiofilm activity (biofilm bacterial killing and reduction of matrix quantity) to CH and has potential to be developed as an intracanal medicament.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 10","pages":"Pages 1412-1419"},"PeriodicalIF":3.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141633618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Compatibility of Dual Rinse 1-Hydroxyethane-1,1-Diphosphonic Acid with Sodium Hypochlorite Solutions Containing Proprietary Additives 双冲洗 HEDP 与含专利添加剂的次氯酸钠溶液的兼容性。
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.joen.2024.07.007
Honey Kottathil MDent , Matthias Zehnder PhD , Andrea Gubler , Thomas Attin PhD , Rajkumar Narkedamalli MDS , Nidambur Vasudev Ballal MDS, PhD

Introduction

A salt of 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP) was commercialized some years ago. This so-called Dual Rinse (DR) HEDP has been tested for its combined actions and interactions with pure sodium hypochlorite (NaOCl) solutions. The aim of this study was to assess DR interactions with NaOCl solutions containing proprietary additives.

Methods

Designated endodontic NaOCl solutions that come at 2 different strengths (2% and 5.25%), and without or with additives (Chloraxid and Chloraxid Extra, respectively) were tested. Some of their key chemical and physical properties were determined in the presence or absence of HEDP (9%) in the solution. In addition, soft tissue dissolution efficacy of test and control solutions was assessed in extracted teeth (n = 10 per group, 90 teeth in total).

Results

The NaOCl content in the 2% Chloraxid solutions was slightly above, that in the 5.25% solutions below the labeled concentration. The additives in the Chloraxid Extra solutions neither altered their surface tension nor their viscosity. The addition of HEDP to any of the tested NaOCl solutions reduced their pH, and increased their surface tension and viscosity. HEDP-induced available chlorine loss over the first hour was similar between all NaOCl solutions. Soft tissue dissolution in the root canals was affected by NaOCl concentration, but neither by any proprietary NaOCl additive in the Chloraxid solutions, nor the addition of DR HEDP.

Conclusions

Proprietary additives had no influence on any of the tested parameters, including surface tension of the "Extra" solutions. The increased viscosity of combined NaOCl/HEDP solutions deserves further attention.
简介1-hydroxyethylidene-1,1-diphosphonic acid(1-羟基亚乙基-1,1-二膦酸)的一种盐已于几年前商品化。已对这种所谓的双冲洗 HEDP 的综合作用以及与纯 NaOCl 溶液的相互作用进行了测试。本研究的目的是评估双冲洗剂与含有专利添加剂的 NaOCl 溶液之间的相互作用:对指定的牙髓NaOCl溶液进行了测试,这些溶液有两种不同的浓度(2% 和 5.25%),分别不含或含添加剂(Chloraxid 和 Chloraxid Extra)。在溶液中存在或不存在 HEDP(9%)的情况下,对它们的一些关键化学和物理特性进行了测定。此外,还在拔出的牙齿(每组 10 颗,共 90 颗牙齿)中评估了测试溶液和对照溶液的软组织溶解功效:结果:2% Chloraxid 溶液中的 NaOCl 含量略高于标示浓度,而 5.25% 溶液中的 NaOCl 含量低于标示浓度。特级氯消毒剂溶液中的添加剂既不会改变其表面张力,也不会改变其粘度。在任何一种测试过的 NaOCl 溶液中加入 HEDP 都会降低其 pH 值,增加其表面张力和粘度。所有 NaOCl 溶液在第一小时内由 HEDP 引起的可用氯损失相似。根管中软组织的溶解受 NaOCl 浓度的影响,但既不受 Chloraxid 溶液中任何 NaOCl 专有添加剂的影响,也不受添加 Dual Rinse HEDP 的影响:结论:专有添加剂对任何测试参数都没有影响,包括 "额外 "溶液的表面张力。NaOCl/HEDP 混合溶液的粘度增加值得进一步关注。
{"title":"Compatibility of Dual Rinse 1-Hydroxyethane-1,1-Diphosphonic Acid with Sodium Hypochlorite Solutions Containing Proprietary Additives","authors":"Honey Kottathil MDent ,&nbsp;Matthias Zehnder PhD ,&nbsp;Andrea Gubler ,&nbsp;Thomas Attin PhD ,&nbsp;Rajkumar Narkedamalli MDS ,&nbsp;Nidambur Vasudev Ballal MDS, PhD","doi":"10.1016/j.joen.2024.07.007","DOIUrl":"10.1016/j.joen.2024.07.007","url":null,"abstract":"<div><h3>Introduction</h3><div>A salt of 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP) was commercialized some years ago. This so-called Dual Rinse (DR) HEDP has been tested for its combined actions and interactions with pure sodium hypochlorite (NaOCl) solutions. The aim of this study was to assess DR interactions with NaOCl solutions containing proprietary additives.</div></div><div><h3>Methods</h3><div>Designated endodontic NaOCl solutions that come at 2 different strengths (2% and 5.25%), and without or with additives (Chloraxid and Chloraxid Extra, respectively) were tested. Some of their key chemical and physical properties were determined in the presence or absence of HEDP (9%) in the solution. In addition, soft tissue dissolution efficacy of test and control solutions was assessed in extracted teeth (<em>n</em> = 10 per group, 90 teeth in total).</div></div><div><h3>Results</h3><div>The NaOCl content in the 2% Chloraxid solutions was slightly above, that in the 5.25% solutions below the labeled concentration. The additives in the Chloraxid Extra solutions neither altered their surface tension nor their viscosity. The addition of HEDP to any of the tested NaOCl solutions reduced their pH, and increased their surface tension and viscosity. HEDP-induced available chlorine loss over the first hour was similar between all NaOCl solutions. Soft tissue dissolution in the root canals was affected by NaOCl concentration, but neither by any proprietary NaOCl additive in the Chloraxid solutions, nor the addition of DR HEDP.</div></div><div><h3>Conclusions</h3><div>Proprietary additives had no influence on any of the tested parameters, including surface tension of the \"Extra\" solutions. The increased viscosity of combined NaOCl/HEDP solutions deserves further attention.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 10","pages":"Pages 1472-1477"},"PeriodicalIF":3.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Surface Modification of Dentin Powder With Alginate and Evaluation of Its Effects on the Viability and Proliferation of Dental Pulp Stem Cells (In Vitro), Its Biocompatibility (In Vivo) 用海藻酸盐对牙本质粉进行表面改性,并评估其对牙髓干细胞的活力和增殖(体外)及其生物相容性(体内)的影响。
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.joen.2024.07.015
Melika Manzarpour DDS , Mohammad Reza Mousavi DDS , Yas Mahdavinaderi DDS , Mohammadali Najimi DDS , Amin Ghalambor DDS , Sadegh Hasannia PhD , Sarah Rajabi PhD , Mohamad Pezeshki-Modaress PhD , Amir Kamali PhD , Hengameh Bakhtiar DDS, MSc, FRCD

Introduction

This study aimed to synthesize dentin powder surface modified with alginate, a potential substance for dental pulp regeneration, and evaluate its effects on the viability and proliferation of human dental pulp stem cells in vitro and its biocompatibility in vivo.

Methods

In the in vitro phase, dentin powder was synthesized in 3 size groups (150–250 μm, 250–500 μm, and 500–1000 μm) after demineralization and atelopeptidization which is used to remove dentin collagen telopeptides and eliminate host immune response. Surface modification with alginate was performed and followed by field-emission scanning electron microscopy, energy dispersive X-ray spectroscopy, and cell viability and proliferation testing for 14 days with human dental pulp stem cells studied. In the in vivo phase, dentin powders were implanted in rat calvarial defects for 8 weeks, and histologic analysis was conducted. All nonparametric data were analyzed with the Kruskal–Wallis test, and all the quantitative data were analyzed by 1-way analysis of variance using SPSS, and P < .05 was considered statistically significant.

Results

Demineralization and atelopeptidization were successful in all groups. Cell viability was optimal and equal (P > .05) in all groups. The 500- to 1000-μm group exhibited significantly higher cell proliferation (P < .05). Histologic assessment shows acceptable biocompatibility in all groups; the angiogenesis score was significantly greater in both 250–500 and 500–1000, and minimal inflammatory response was noted in the 500- to 1000-μm group, and the amount of newly formed bone in this group was higher than other groups.

Conclusions

Surface modification of demineralized and atelopeptidized dentin powder with alginate enhanced surface physical properties and cell proliferation while showing great biocompatibility within tissue and reducing the host immune response. These findings hold promise for dentin-pulp complex regeneration.
简介:本研究旨在合成海藻酸盐表面修饰的牙本质粉末(一种潜在的牙髓再生物质),并评估其在体外对人牙髓干细胞(hDPSCs)的活力和增殖的影响及其在体内的生物相容性:在体外阶段,将牙本质粉末合成为三个尺寸组(150-250 μm、250-500 μm、500-1000 μm),然后进行脱矿和羚肽化处理,以去除牙本质胶原端肽并消除宿主免疫反应。用海藻酸盐进行表面改性后,用场发射扫描电子显微镜(FE-SEM)、能量色散 X 射线光谱(EDX)和细胞活力与增殖测试对 hDPSCs 进行了 14 天的研究。在体内阶段,将牙本质粉植入大鼠腓骨缺损处 8 周,并进行组织学分析。所有非参数数据均采用 Kruskal- Wallis 检验进行分析,所有定量数据均采用 SPSS 和 PResults 进行单因素方差分析:各组脱钙和脱肽均成功。各组细胞存活率最佳且相同(P>0.05)。500-1000 μm 组的细胞增殖率明显更高(PC结论:去矿物质和阿特肽的表面改性在所有组中都是成功的:用海藻酸盐对脱矿和阿特肽化的牙本质粉进行表面修饰,可增强表面物理性质和细胞增殖,同时在组织内显示出良好的生物相容性,并降低宿主免疫反应。这些发现为牙本质-牙髓复合体的再生带来了希望。
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引用次数: 0
Radiographic Evaluation of Regenerative Endodontic Procedures and Apexification Treatments with the Assessment of External Root Resorption 通过评估外根吸收对牙髓再生程序和根尖化治疗进行放射学评估。
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.joen.2024.06.003

Introduction

This multicentered cohort study evaluated the radiographic outcomes of regenerative endodontic procedures (REPs) and apexification treatments (APEX) of immature teeth with endodontic disease.

Materials and Methods

This cohort study included a retrospective record review and prospective data collection of pediatric patients with teeth treated with REPs or APEX between 2005 and 2014. Data including the presence of a periapical lesion, external root resorption, obliteration, apical hard tissue, apical closure, intracanal calcifications, and radiographic root area (RRA) change based on measurements were collected/measured from radiographic images. Univariate and multivariate analyses were conducted.

Results

The cohort included 190 subjects (204 teeth (92 REPs; 112 APEX)). The frequency of pretreatment periapical pathology was similar between cases in which the clinical treatment failed versus successful treatment cases. However, the frequency of pretreatment external root resorption was higher in failed cases than in successful cases (P = .007). The mean RRA change was greater than twenty percent in 21% of the REPs cases. In traumatized teeth, REPs resulted in less hard tissue formation than other endodontic disease etiologies measured by RRA (P = .001). Fifty-three percent of cases with ERR (16/30) showed signs of healing/arrest and were mostly treated with REPs (11/16).

Conclusions

The presence of ERR negatively affected the treatment outcome. There was significant variability in RRA change in REPs. Signs of healing/arrest of the resorptive lesion were radiographically visible in many cases treated with REPs.
简介:这项多中心队列研究评估了对患有牙髓病的未成熟牙齿进行再生根管治疗(REPs)和顶点化治疗(APEX)的影像学结果:这项队列研究包括对 2005-2014 年间接受 REP 或 APEX 治疗的儿童患者进行回顾性记录审查和前瞻性数据收集。数据包括根尖周病变的存在、外根吸收(ERR)、阻塞、根尖硬组织、根尖闭合、根管内钙化以及根据放射影像测量的放射根面积(RRA)变化。进行了单变量和多变量分析:研究对象包括 190 名受试者(204 颗牙齿(92 颗 REP;112 颗 APEX))。临床治疗失败的病例与治疗成功的病例在治疗前出现根尖周病变的频率相似。但是,治疗失败病例治疗前出现ERR的频率高于治疗成功病例(P=0.007)。在 21% 的 REP 病例中,平均 RRA 变化超过 20%。在受创伤的牙齿中,REP 治疗导致的硬组织形成少于 RRA 测量的其他牙髓病病因(p=0.001)。53%的ERR病例(16/30)显示出愈合/停滞的迹象,大部分病例接受了REP治疗(11/16):结论:ERR的存在对治疗效果有负面影响。结论:ERR的存在对治疗结果产生了负面影响,REPs的RRA变化差异很大。在许多使用 REP 治疗的病例中,吸收性病变的愈合/恢复迹象在影像学上是可见的。
{"title":"Radiographic Evaluation of Regenerative Endodontic Procedures and Apexification Treatments with the Assessment of External Root Resorption","authors":"","doi":"10.1016/j.joen.2024.06.003","DOIUrl":"10.1016/j.joen.2024.06.003","url":null,"abstract":"<div><h3>Introduction</h3><div>This multicentered cohort study evaluated the radiographic outcomes of regenerative endodontic procedures (REPs) and apexification treatments (APEX) of immature teeth with endodontic disease.</div></div><div><h3>Materials and Methods</h3><div>This cohort study included a retrospective record review and prospective data collection of pediatric patients with teeth treated with REPs or APEX between 2005 and 2014. Data including the presence of a periapical lesion, external root resorption, obliteration, apical hard tissue, apical closure, intracanal calcifications, and radiographic root area (RRA) change based on measurements were collected/measured from radiographic images. Univariate and multivariate analyses were conducted.</div></div><div><h3>Results</h3><div>The cohort included 190 subjects (204 teeth (92 REPs; 112 APEX)). The frequency of pretreatment periapical pathology was similar between cases in which the clinical treatment failed versus successful treatment cases. However, the frequency of pretreatment external root resorption was higher in failed cases than in successful cases (<em>P</em> = .007). The mean RRA change was greater than twenty percent in 21% of the REPs cases. In traumatized teeth, REPs resulted in less hard tissue formation than other endodontic disease etiologies measured by RRA (<em>P</em> = .001). Fifty-three percent of cases with ERR (16/30) showed signs of healing/arrest and were mostly treated with REPs (11/16).</div></div><div><h3>Conclusions</h3><div>The presence of ERR negatively affected the treatment outcome. There was significant variability in RRA change in REPs. Signs of healing/arrest of the resorptive lesion were radiographically visible in many cases treated with REPs.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 10","pages":"Pages 1420-1428.e1"},"PeriodicalIF":3.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141327551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dens invaginatus Type II in an Immature Lateral Incisor With Apical Periodontitis Treated With Regenerative and Strategic Antimicrobial Procedures: A Case Report 用再生和策略性抗菌疗法治疗一颗患有根尖牙周炎的未成熟侧切牙的Ⅱ型牙槽窝:一份病例报告
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.joen.2024.07.004
Gabriela Martin DDS, MSc, PhD , Florencia Cires DDS , Jorge Alberdi DDS, PhD , Cecilia I. Rourera DDS , Claudia Bonnin DDS, PhD , Isabela N. Rôças DDS, MSc, PhD , José F. Siqueira Jr DDS, MSc, PhD
Dens invaginatus is a developmental dental anomaly that can predispose the tooth to pulp and periradicular disease. Management of this condition can be challenging because of anatomic and microbiologic issues. This case report describes the regenerative endodontic treatment using a strategic antimicrobial protocol for management of an immature maxillary lateral incisor with type-II dens invaginatus associated with apical periodontitis in a 13-year-old patient. The tooth presented with a complex anatomy and was associated with an active sinus tract. Because the true root canal was not negotiable in its coronal part due to the presence of the dens invaginatus, the closed end of the invagination (pseudocanal) was perforated to permit access to the apical segment of the true root canal for cleaning and disinfection. Both the invagination and the true canal were treated using an antimicrobial regimen based on chemomechanical preparation with sodium hypochlorite irrigation, supplementary disinfection with passive ultrasonic irrigation and interappointment calcium hydroxide medication. After 2 exchanges of calcium hydroxide medication, the sinus tract did not disappear, then the antimicrobial protocol was changed to include an antibiotic solution for irrigation and antibiotic paste for intracanal medication. After signs and symptoms disappeared, regenerative endodontic treatment was performed by inducing blood clot formation within the root canal. The coronal canal segment including the invagination was filled with Biodentine. Follow-up including cone-beam computed tomography examination showed complete healing of the apical periodontitis lesion and mineralized tissue formation at the apical portion of the true root canal.
内陷牙是一种牙齿发育异常,容易导致牙髓和根周疾病。由于解剖学和微生物学方面的问题,这种情况的治疗可能具有挑战性。本病例报告描述了采用策略性抗菌方案进行牙髓再生治疗的情况,该方案用于治疗一名 13 岁患者的上颌未成熟侧切牙 II 型窝洞伴根尖牙周炎。这颗牙齿的解剖结构复杂,并伴有活跃的窦道。由于内陷窝的存在,真根管的冠状部分无法进行切削,因此对内陷窝的封闭端(假根管)进行了穿孔,以便进入真根管的根尖部分进行清洁和消毒。对内陷和真根管都采用了抗菌治疗方案,包括次氯酸钠灌洗的化学机械准备、被动超声波灌洗的辅助消毒以及穿插氢氧化钙药物。换用氢氧化钙药物 2 次后,窦道症状仍未消失,于是改变抗菌方案,将抗生素溶液用于冲洗,抗生素糊剂用于窦内用药。症状和体征消失后,通过诱导根管内血凝块的形成进行了牙髓再生治疗。用 Biodentine 填充了包括内陷在内的冠状根管段。随访包括锥形束计算机断层扫描检查显示,根尖牙周炎病变完全愈合,真根管的根尖部分形成了矿化组织。
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引用次数: 0
A Fluorescence-based Method to Reaccess Root Canals in Endodontically Treated Teeth: A Micro–Computed Tomography Tridimensional Assessment 基于荧光的牙髓治疗牙根管再通路方法:MicroCT 三维评估。
IF 3.5 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.joen.2024.07.009
Iago Ramirez DDS, MSc , Lautaro Gallardo Altube DDS, MSc , Rafael Verardino Camargo DDS, MSc , Alice Corrêa Silva-Sousa DDS, MSc, PhD , Aline Evangelista Souza-Gabriel DDS, MSc, PhD , Liviu Steier DDS, MSc, PhD , Fabiane Carneiro Lopes-Olhê DDS, MSc, PhD , Manoel Damião Sousa-Neto DDS, MSc, PhD , Jardel Francisco Mazzi-Chaves DDS, MSc, PhD

Introduction

The aim of this study was to evaluate the volume of dentin removal and the volume of remnants of restorative material after the removal of an esthetic restorative coronal set and cervical barrier in endodontically treated mandibular molars with the aid of different magnification methods using 3-dimensional (3D) micro–computed tomographic (micro-CT) morphometric analysis.

Methods

A sample of 30 mandibular first molars (N = 30) was used. All teeth were endodontically treated, and the specimens were initially scanned using micro-CT imaging and reconstructed. The molars were filled by a single-cone technique, and immediately the material at the initial 2-mm cervical level was removed. Cervical barriers were confected using ionomer glass cement with fluorescein 0.1%, filling the 2 mm at the cervical level of the canals and an additional 2 mm as the base. The coronal restoration set was performed using esthetic resin composites. A simulated tooth aging process was performed with 20,000 thermocycling cycles. The sample was distributed into the following 3 groups (n = 10) for the removal of the restoration set and cervical barrier with diamond burs based on the magnification aid: no magnification aid (naked eye), operative microscope aid, and REVEAL device (Design for Vision Inc, Bohemia, NY) aid. After removal, the final 3D micro-CT scanning and reconstruction were conducted with the same parameters as the initial scanning, and superposition of the final and initial scanning was performed. Morphometric analysis was conducted using CTAn software (Bruker microCT, Kontich, Belgium) to assess the volume of remnant restorative material (mm³), the volume of dentin removal (mm³), and the direction and site of dentin removal. Data were analyzed using 1-Way analysis of variance (P < .05).

Results

The REVEAL group showed better results regarding the volume of remnant material (3.17 ± 1.65) and the percentage of dentin removal (2.56 ± 1.34). The microscope group showed no statistical difference compared with the REVEAL group regarding dentin removal (3.30 ± 1.48) and was statistically similar to the naked eye group in the volume of remnant material (9.63 ± 4.33). The naked eye group showed the worst results for the volume of remnant material (7.60 ± 2.68) and the percentage of dentin removal (6.60 ± 3.70).

Conclusions

The use of fluorescence associated with magnification was the method that presented the best results, with lower percentages of dentin removal and smaller volumes of remaining restorative material. This is an innovative technology in endodontics that shows potential to overcome the challenge of reaccessing root canals in the context of endodontic retreatment.
目的:利用三维显微CT形态计量分析法,借助不同的放大方法,评估牙髓治疗下颌磨牙在去除美观修复冠套和牙颈部屏障后的牙本质去除量和修复材料残留量:方法:取样 30 颗下颌第一磨牙(n=30)。方法:使用 30 颗下颌第一磨牙样本(n=30),所有牙齿均经过牙髓治疗,标本在 MicroCT 中进行了初步扫描和重建。臼齿采用单锥技术充填,并立即去除最初 2 毫米颈水平的材料。使用添加了 0.1%荧光素的离子玻璃粘固剂制作牙颈部屏障,充填牙颈部 2 毫米的牙槽,并以另外 2 毫米作为基底。冠状修复使用美学树脂复合材料。模拟牙齿老化过程的热循环次数为 20,000 次。将样本分为三组(n=10),根据放大辅助工具,用金刚石车针去除修复体和牙颈部屏障:无放大辅助设备(肉眼);手术显微镜辅助设备;REVEAL™ 设备辅助设备。取出后,以与初始扫描相同的参数进行最终 3D MicroCT 扫描和重建,并对最终扫描和初始扫描进行叠加。使用 CTAn 进行形态计量分析,以评估残余修复材料的体积(立方毫米)、牙本质去除体积(立方毫米)以及牙本质去除的方向和部位。数据采用单向方差分析(pResults:REVEAL™ 组在残余材料量(3.17 ± 1.65)和牙本质去除率(2.56 ± 1.34)方面表现更好。显微镜组与 REVEAL™ 组在牙本质去除率(3.30 ± 1.48)方面没有统计学差异,在残余材料量(9.63 ± 4.33)方面与裸眼组在统计学上相似。裸眼组的残余材料量(7.60 ± 2.68)和牙本质去除百分比(6.60 ± 3.70)结果最差:结论:使用荧光和放大镜的方法效果最好,牙本质去除率较低,剩余修复材料较少。这是牙髓病学中的一项创新技术,它显示出在牙髓再治疗中克服根管再通路挑战的潜力。
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引用次数: 0
期刊
Journal of endodontics
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