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Randomized Clinical Trial: Bone Bioactive Liquid Improves Implant Stability and Osseointegration. 随机临床试验:骨生物活性液能改善种植体的稳定性和骨结合。
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-01 DOI: 10.3390/jfb15100293
Ashraf Al Madhoun, Khaled Meshal, Neus Carrió, Eduard Ferrés-Amat, Elvira Ferrés-Amat, Miguel Barajas, Ana Leticia Jiménez-Escobar, Areej Said Al-Madhoun, Alaa Saber, Yazan Abou Alsamen, Carles Marti, Maher Atari

Implant stability can be compromised by factors such as inadequate bone quality and infection, leading to potential implant failure. Ensuring implant stability and longevity is crucial for patient satisfaction and quality of life. In this multicenter, randomized, double-blind clinical trial, we assessed the impact of a bone bioactive liquid (BBL) on the Galaxy TS implant's performance, stability, and osseointegration. We evaluated the impact stability, osseointegration, and pain levels using initial stability quotient (ISQ) measurements, CBCT scans, and pain assessment post-surgery. Surface analysis was performed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). In vitro studies examined the BBL's effects on dental pulp pluripotent stem cells' (DPPSCs') osteogenesis and inflammation modulation in human macrophages. All implants successfully osseointegrated, as demonstrated by the results of our clinical and histological studies. The BBL-treated implants showed significantly lower pain scores by day 7 (p < 0.00001) and improved stability by day 30 (ISQ > 62.00 ± 0.59, p < 8 × 10-7). By day 60, CBCT scans revealed an increased bone area ratio in BBL-treated implants. AFM images demonstrated the BBL's softening and wettability effect on implant surfaces. Furthermore, the BBL promoted DPPSCs' osteogenesis and modulated inflammatory markers in human primary macrophages. This study presents compelling clinical and biological evidence that BBL treatment improves Galaxy TS implant stability, reduces pain, and enhances bone formation, possibly through surface tension modulation and immunomodulatory effects. This advancement holds promise for enhancing patient outcomes and implant longevity.

骨质不足和感染等因素会影响种植体的稳定性,从而导致种植失败。确保种植体的稳定性和使用寿命对患者的满意度和生活质量至关重要。在这项多中心、随机、双盲临床试验中,我们评估了骨生物活性液体(BBL)对 Galaxy TS 种植体的性能、稳定性和骨结合的影响。我们通过初始稳定商数(ISQ)测量、CBCT 扫描和术后疼痛评估来评估植入物的稳定性、骨结合性和疼痛程度。我们使用扫描电子显微镜(SEM)和原子力显微镜(AFM)进行了表面分析。体外研究检验了 BBL 对牙髓多能干细胞(DPPSCs)成骨和人类巨噬细胞炎症调节的影响。我们的临床和组织学研究结果表明,所有种植体都成功实现了骨结合。经 BBL 处理的种植体在第 7 天时疼痛评分明显降低(p < 0.00001),在第 30 天时稳定性提高(ISQ > 62.00 ± 0.59,p < 8 × 10-7)。到第 60 天,CBCT 扫描显示 BBL 处理过的种植体的骨面积比有所增加。原子力显微镜图像显示了 BBL 对种植体表面的软化和润湿效果。此外,BBL 还促进了 DPPSCs 的成骨过程,并调节了人类原代巨噬细胞中的炎症标志物。这项研究提供了令人信服的临床和生物学证据,证明 BBL 治疗可改善 Galaxy TS 种植体的稳定性、减轻疼痛并促进骨形成,这可能是通过表面张力调节和免疫调节作用实现的。这一进步有望提高患者的治疗效果和种植体的使用寿命。
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引用次数: 0
The Effect of Erosive Media on the Mechanical Properties of CAD/CAM Composite Materials. 腐蚀介质对 CAD/CAM 复合材料机械性能的影响。
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-10-01 DOI: 10.3390/jfb15100292
Marwa M Alnsour, Rasha A Alamoush, Nikolaos Silikas, Julian D Satterthwaite

This study aimed to investigate the effect of acidic media storage (gastric acid and Coca-Cola) on the mechanical properties of CAD/CAM materials. Three types of materials were tested: a polymer-infiltrated ceramic network (PICN) (Vita Enamic (En), VITA Zahnfabrik, Germany), a resin composite block (RCB) (Cerasmart (Cs), GC Corp, Japan), and a conventional resin-based composite (Gradia direct (Gr), GC Corp, Japan), which was used as a control. Beam-shaped specimens of each material, with dimensions of 16 mm × 4 mm × 1.5 mm, were prepared (90 in total). The specimens were divided into subgroups (10 each) and stored for 96 h in either gastric acid, Coca-Cola, or distilled water. Flexural strength and elastic modulus were evaluated using a three-point flexural strength test with acoustic emission (AE) monitoring. Vickers microhardness was measured before and after storage in gastric acid and Coca-Cola. Data were statistically analysed using two-way and one-way ANOVA, the Tukey's post hoc, and independent t-test at a significance level of 0.05. The results showed that Cs and En maintained their flexural strength and elastic modulus after acidic media exposure, while Gr experienced a significant decrease in flexural strength following gastric acid storage (p < 0.01). Initial crack detection was not possible using the AE system, impacting the determination of flexural strength. Exposure to acidic media decreased all materials' microhardness, with Gr showing the most notable reduction (p < 0.0001). Gastric acid had a greater impact on the microhardness of all tested materials compared to Coca-Cola (p < 0.0001). In conclusion, storage in erosive media did not notably affect the flexural strength or elastic modulus of CAD/CAM composites but it did affect hardness. CAD/CAM composite blocks demonstrated superior mechanical properties compared to the conventional composite.

本研究旨在探讨酸性介质储存(胃酸和可口可乐)对 CAD/CAM 材料机械性能的影响。测试了三种材料:聚合物浸润陶瓷网络(PICN)(Vita Enamic (En),德国 VITA Zahnfabrik 公司)、树脂复合材料块(RCB)(Cerasmart (Cs),日本 GC 公司)和作为对照的传统树脂复合材料(Gradia direct (Gr),日本 GC 公司)。每种材料都制备了尺寸为 16 mm × 4 mm × 1.5 mm 的梁状试样(共 90 个)。将试样分成若干组(每组 10 个),在胃酸、可口可乐或蒸馏水中存放 96 小时。采用声发射(AE)监测的三点抗弯强度测试评估抗弯强度和弹性模量。在胃酸和可口可乐中储存前后测量了维氏硬度。数据采用双向和单向方差分析、Tukey 后检验和独立 t 检验进行统计分析,显著性水平为 0.05。结果表明,Cs 和 En 在接触酸性介质后保持了抗折强度和弹性模量,而 Gr 在胃酸储存后抗折强度显著下降(p < 0.01)。使用 AE 系统无法进行初始裂纹检测,这影响了抗弯强度的测定。暴露在酸性介质中会降低所有材料的显微硬度,其中 Gr 的降低最为明显(p < 0.0001)。与可口可乐相比,胃酸对所有测试材料显微硬度的影响更大(p < 0.0001)。总之,在侵蚀性介质中储存不会明显影响 CAD/CAM 复合材料的抗弯强度或弹性模量,但会影响硬度。与传统复合材料相比,CAD/CAM 复合材料砌块表现出更优越的机械性能。
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引用次数: 0
Preparation, Mechanical Properties, and Degradation Behavior of Zn-1Fe-xSr Alloys for Biomedical Applications. 用于生物医学应用的 Zn-1Fe-xSr 合金的制备、机械性能和降解行为。
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-30 DOI: 10.3390/jfb15100289
Wen Peng, Zehang Lu, Enyang Liu, Wenteng Wu, Sirong Yu, Jie Sun

As biodegradable materials, zinc (Zn) and zinc-based alloys have attracted wide attention owing to their great potential in biomedical applications. However, the poor strength of pure Zn and binary Zn alloys limits their wide application. In this work, a stir casting method was used to prepare the Zn-1Fe-xSr (x = 0.5, 1, 1.5, 2 wt.%) ternary alloys, and the phase composition, microstructure, tensile properties, hardness, and degradation behavior were studied. The results indicated that the SrZn13 phase was generated in the Zn matrix when the Sr element was added, and the grain size of Zn-1Fe-xSr alloy decreased with the increase in Sr content. The ultimate tensile strength (UTS) and Brinell hardness increased with the increase in Sr content. The UTS and hardness of Zn-1Fe-2Sr alloy were 141.65 MPa and 87.69 HBW, which were 55.7% and 58.4% higher than those of Zn-1Fe alloy, respectively. As the Sr content increased, the corrosion current density of Zn-1Fe-xSr alloy increased, and the charge transfer resistance decreased significantly. Zn-1Fe-2Sr alloy had a degradation rate of 0.157 mg·cm-2·d-1, which was 118.1% higher than the degradation rate of Zn-1Fe alloy. Moreover, the degradation rate of Zn-1Fe-xSr alloy decreased significantly with the increase in immersion time.

作为可生物降解材料,锌(Zn)和锌基合金因其在生物医学应用中的巨大潜力而受到广泛关注。然而,纯锌和二元锌合金的强度较低,限制了它们的广泛应用。本研究采用搅拌铸造法制备了 Zn-1Fe-xSr(x = 0.5、1、1.5、2 wt.%)三元合金,并对其相组成、显微结构、拉伸性能、硬度和降解行为进行了研究。结果表明,加入 Sr 元素后,Zn 基体中生成了 SrZn13 相,Zn-1Fe-xSr 合金的晶粒尺寸随 Sr 含量的增加而减小。极限拉伸强度(UTS)和布氏硬度随 Sr 含量的增加而增加。Zn-1Fe-2Sr 合金的 UTS 和硬度分别为 141.65 MPa 和 87.69 HBW,比 Zn-1Fe 合金分别高 55.7% 和 58.4%。随着 Sr 含量的增加,Zn-1Fe-xSr 合金的腐蚀电流密度增大,电荷转移电阻明显下降。Zn-1Fe-2Sr 合金的降解率为 0.157 mg-cm-2-d-1,比 Zn-1Fe 合金的降解率高 118.1%。此外,随着浸泡时间的延长,Zn-1Fe-xSr 合金的降解率显著下降。
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引用次数: 0
The Integration of Gold Nanoparticles into Dental Biomaterials as a Novel Approach for Clinical Advancement: A Narrative Review. 将纳米金粒子融入牙科生物材料是促进临床发展的新方法:叙述性综述。
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-30 DOI: 10.3390/jfb15100291
Saharat Jongrungsomran, Dakrong Pissuwan, Apichai Yavirach, Chaiy Rungsiyakull, Pimduen Rungsiyakull

Gold nanoparticles (AuNPs) have gained significant attention in the biomedical field owing to their versatile properties. AuNPs can be customized by modifying their size, shape and surface characteristics. In recent years, extensive research has explored the integration of AuNPs into various dental materials, including titanium, polymethylmethacrylate (PMMA) and resin composites. This review aims to summarize the advancements in the application of modified AuNPs in dental materials and to assess their effects on related cellular processes in the dental field. Relevant articles published in English on AuNPs in association with dental materials were identified through a systematic search of the PubMed/MEDLINE, Embase, Scopus and ScienceDirect databases from January 2014 to April 2024. Future prospects for the utilization of AuNPs in the field of dentistry are surveyed.

金纳米粒子(AuNPs)因其多功能特性在生物医学领域备受关注。AuNPs 可通过改变其大小、形状和表面特征进行定制。近年来,人们对 AuNPs 与各种牙科材料(包括钛、聚甲基丙烯酸甲酯(PMMA)和树脂复合材料)的结合进行了广泛的研究。本综述旨在总结改性 AuNPs 在牙科材料中的应用进展,并评估其对牙科领域相关细胞过程的影响。通过系统检索PubMed/MEDLINE、Embase、Scopus和ScienceDirect数据库,确定了2014年1月至2024年4月期间发表的有关AuNPs与牙科材料相关的英文文章。对金纳米粒子在牙科领域的应用前景进行了调查。
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引用次数: 0
Correction: Immich et al. Evaluation of Antimicrobial Properties, Cell Viability, and Metalloproteinase Activity of Bioceramic Endodontic Materials Used in Vital Pulp Therapy. J. Funct. Biomater. 2024, 15, 70. 更正:Immich等人.评估活力牙髓疗法中使用的生物陶瓷牙髓材料的抗菌特性、细胞活力和金属蛋白酶活性.J. Funct.Biomater.2024, 15, 70.
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-30 DOI: 10.3390/jfb15100290
Felipe Immich, Durvalino de Oliveira, Juliana Silva Ribeiro de Andrade, Andressa da Silva Barboza, Carlos Enrique Cuevas-Suárez, Adriana Fernandes da Silva, Wellington Luiz de Oliveira da Rosa, Álvaro Henrique Borges, Neftali Lenin Villarreal Carreno, Evandro Piva, Rafael Guerra Lund

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图 3 中的错误 [...].
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引用次数: 0
Evaluation of the Peri-Implant Tissues of Patients with Severe Bone Atrophy Treated with a New Short and Extra-Short Implant System-A Pilot Study. 使用新型短型和超短型种植体系统治疗严重骨质萎缩患者的种植体周围组织评估--一项试点研究。
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-29 DOI: 10.3390/jfb15100288
Kely Cristina de Moraes, Geninho Thomé, Flávia Noemy Gasparini Kiatake Fontão, Carolina Accorsi Cartelli, Rosemary Adriana Chierici Marcantonio, Carolina Mendonça de Almeida Malzoni, Elcio Marcantonio Junior

This study aimed to assess clinical and radiographic outcomes, including implant survival, marginal bone loss, and patient satisfaction, in individuals with severe bone atrophy treated using a newly developed system of short and extra-short implants. A total of 44 implants (37 short and 7 extra-short) were placed with immediate loading in 11 patients. The patients were followed up at between 6 and 24 months. Bone changes, keratinized mucosa, bleeding on probing, probing depth, crown-to-implant ratio, and patient satisfaction were evaluated. An implant survival and success rate of 100% was observed. The peri-implant bone condition showed no significant associations between marginal bone loss (MBL) and gingival recession. In extra-short implants, the crown-to-implant ratio did not affect MBL in the evaluated times. However, short implants showed a statistically significant inverse correlation between mesial measurement and crown-to-implant ratio (p = 0.006) and between distal measurement and crown-to-implant ratio (p = 0.004) over six months. Plaque was present in the mesiobuccal regions in 38.64% of the implants, with extra-short implants having the highest relative frequency (71.4%). Bleeding was observed in 18.9% of the short implants in the mesiolingual region and 14.3% of the extra-short implants. There was a statistically significant association between bleeding on probing in the mesiobuccal region and the type of implant (p = 0.026). The analysis of probing depth showed no difference between the types of implants. Within the limits of this study, short and extra-short implants presented similar clinical and radiographic behavior of soft and hard tissues in the evaluated times.

这项研究旨在评估使用新开发的短种植体和超短种植体系统治疗严重骨萎缩患者的临床和影像学效果,包括种植体存活率、边缘骨损失和患者满意度。共为 11 名患者植入了 44 个种植体(37 个短种植体和 7 个超短种植体),并进行了即刻加载。对患者进行了 6 至 24 个月的随访。对骨质变化、角化粘膜、探查出血、探查深度、牙冠与种植体比例以及患者满意度进行了评估。结果显示,种植体的存活率和成功率均为 100%。种植体周围的骨质状况显示,边缘骨质流失(MBL)和牙龈退缩之间没有明显的关联。在超短种植体中,牙冠与种植体的比例在评估时间内不会影响骨质疏松。但是,短种植体在 6 个月内的中轴测量值与牙冠与种植体比值之间(p = 0.006)以及远轴测量值与牙冠与种植体比值之间(p = 0.004)存在统计学意义上的显著负相关。38.64%的种植体的中颊面区域存在牙菌斑,其中超短种植体出现牙菌斑的频率最高(71.4%)。18.9%的短种植体在中叶区域出现出血,14.3%的超短种植体出现出血。中颊面区探诊出血与种植体类型之间有统计学意义(p = 0.026)。对探诊深度的分析表明,不同类型的种植体之间没有差异。在本研究的范围内,短种植体和超短种植体在软组织和硬组织的临床和影像学表现在评估时间上相似。
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引用次数: 0
Antibacterial Biocomposite Based on Chitosan/Pluronic/Agarose Noncovalent Hydrogel: Controlled Drug Delivery by Alginate/Tetracycline Beads System. 基于壳聚糖/丙酮/琼脂糖非共价水凝胶的抗菌生物复合材料:藻酸/四环素珠系统的可控药物输送
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-28 DOI: 10.3390/jfb15100286
Hossein Abdollahi, Saber Amiri, Farzaneh Amiri, Somayeh Moradi, Payam Zarrintaj

Designing a wound dressing with controlled uptake, antibacterial, and proper biocompatibility is crucial for the appropriate wound healing process. In this study, alginate/tetracycline (Alg/TC) beads were produced and embedded into chitosan/pluronic/agarose semi-interpenetrating polymer network hydrogel, which serves as a potential biocompatible dressing for treating skin wounds. The effect of pluronic content on the porosity, swelling, mechanical characteristics, and degradation of the hydrogel was investigated. Furthermore, the impact of Alg beads on TC release was subsequently examined. In the absence of Alg beads, faster release was observed. However, after incorporating beads into the hydrogels, the release was sustained. Particularly, the hydrogel containing Alg beads exhibited a nearly linear release, reaching 74% after 2 days in acidic media. The antimicrobial activity and biocompatibility of the hydrogel were also evaluated to assess the capability of the TC-loaded hydrogels for wound dressing applications. The hydrogel demonstrated efficient antibacterial features against Gram-positive and Gram-negative bacteria. Additionally, the sample behavior was evaluated against exposure to yeast. Furthermore, based on biocompatibility studies using HFF2 cells, the TC-loaded hydrogel exhibited remarkable biocompatibility. Overall, this novel composite hydrogel shows remarkable biocompatibility and antibacterial activities which can be used as a great potential wound dressing to prevent wound infections due to its effective inhibition of bacterial growth.

设计一种具有可控吸收、抗菌和适当生物相容性的伤口敷料对于伤口的适当愈合过程至关重要。本研究制作了海藻酸/四环素(Alg/TC)微珠,并将其嵌入壳聚糖/pluronic/琼脂糖半互穿聚合物网络水凝胶中,作为一种潜在的生物相容性敷料用于治疗皮肤伤口。研究了pluronic含量对水凝胶的孔隙率、膨胀、机械特性和降解的影响。此外,还研究了 Alg 珠对 TC 释放的影响。在没有 Alg 珠的情况下,观察到的释放速度更快。但是,在水凝胶中加入珠子后,释放就会持续。特别是含有 Alg 珠子的水凝胶表现出近乎线性的释放,在酸性介质中 2 天后释放量达到 74%。此外,还对水凝胶的抗菌活性和生物相容性进行了评估,以评估负载 TC 的水凝胶在伤口敷料应用中的能力。该水凝胶对革兰氏阳性和革兰氏阴性细菌具有高效的抗菌特性。此外,还对样品暴露于酵母菌的行为进行了评估。此外,根据使用 HFF2 细胞进行的生物相容性研究,TC 负载水凝胶表现出显著的生物相容性。总之,这种新型复合水凝胶具有出色的生物相容性和抗菌活性,能有效抑制细菌生长,因此可用作一种极具潜力的伤口敷料,防止伤口感染。
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引用次数: 0
Chemically Pretreated Densification of Juniper Wood for Potential Use in Osteosynthesis Bone Implants. 化学预处理桧木致密化,用于骨合成骨植入物的潜力。
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-28 DOI: 10.3390/jfb15100287
Laura Andze, Vadims Nefjodovs, Martins Andzs, Marite Skute, Juris Zoldners, Martins Kapickis, Arita Dubnika, Janis Locs, Janis Vetra

The aim of the study was to perform treatment of juniper wood to obtain wood material with a density and mechanical properties comparable to bone, thus producing a potential material for use in osteosynthesis bone implants. In the first step, partial delignification of wood sample was obtained by Kraft cooking. The second step was extraction with ethanol, ethanol-water mixture, saline, and water to prevent the release of soluble compounds and increase biocompatibility. In the last step, the thermal densification at 100 °C for 24 h was implemented. The results obtained in the dry state are equivalent to the properties of bone. The swelling of chemically pre-treated densified wood was reduced compared to chemically untreated densified wood. Samples showed no cytotoxicity by in vitro cell assays. The results of the study showed that it is possible to obtain noncytotoxic wood samples with mechanical properties equivalent to bones by partial delignification, extraction, and densification. However, further research is needed to ensure the material's shape stability, water resistance, and reduced swelling.

这项研究的目的是对桧木进行处理,以获得密度和机械性能与骨骼相当的木质材料,从而生产出一种可用于骨合成骨植入物的材料。第一步,通过牛皮纸蒸煮法对木材样本进行部分脱木质处理。第二步是用乙醇、乙醇-水混合物、生理盐水和水进行提取,以防止可溶性化合物的释放,提高生物相容性。最后一步是在 100 °C 的温度下进行 24 小时的热致密化处理。在干燥状态下获得的结果与骨的特性相当。与未经化学处理的致密化木材相比,经过化学预处理的致密化木材的膨胀程度有所降低。通过体外细胞检测,样品没有显示出细胞毒性。研究结果表明,通过部分脱木质、提取和致密化处理,可以获得无细胞毒性的木材样本,其机械性能相当于骨骼。不过,要确保材料的形状稳定性、防水性和减少膨胀,还需要进一步的研究。
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引用次数: 0
Customizable Lyophilized Agent for Radiotherapy Imaging and TherapY (CLARITY). 用于放射治疗成像和治疗的可定制冻干制剂(CLARITY)。
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-27 DOI: 10.3390/jfb15100285
Michele Moreau, Debarghya China, Gnagna Sy, Kai Ding, Wilfred Ngwa

Smart radiotherapy biomaterials (SRBs) include seed and liquid biomaterials designed to be employed as fiducial markers during radiotherapy while also delivering therapeutic drug payloads to enhance treatment outcomes. In this study, we investigate a novel Customizable Lyophilized Agent for Radiotherapy Imaging and TherapY (CLARITY) biomaterial, which can be loaded with immunoadjuvants (anti-CD40 monoclonal antibody or Caflanone (FBL-03G)) at the point of care. The CLARITY biomaterial was investigated in an animal model of pancreatic cancer using C57BL6 mice. Mice were imaged before and at different points of time post-treatment to evaluate the potential of CLARITY biomaterial to provide imaging contrast similar to fiducials. This study also used cadavers to assess CLARITY's potential to provide imaging contrast in humans. Results showed imaging contrast from computed tomography (CT) and magnetic resonance imaging (MRI) modalities for up to 30 days post-treatment, demonstrating potential for use as fiducials. A significant increase in survival (***, p = 0.0006) was observed for mice treated with CLARITY biomaterial loaded with immunoadjuvant for up to 10 weeks post-treatment compared to those without treatment. These initial results demonstrate the potential of CLARITY biomaterial to serve as a smart multifunctional radiotherapy biomaterial and provide the impetus for further development and optimization as a point-of-care technology for combination radiotherapy and immunotherapy.

智能放疗生物材料(SRBs)包括种子和液体生物材料,其设计目的是在放疗过程中用作靶标,同时输送治疗药物载荷以提高治疗效果。在本研究中,我们研究了一种新型可定制的用于放疗成像和治疗的冻干制剂(CLARITY)生物材料,它可以在治疗点装载免疫佐剂(抗CD40单克隆抗体或卡夫酮(FBL-03G))。在胰腺癌动物模型中使用 C57BL6 小鼠对 CLARITY 生物材料进行了研究。在治疗前和治疗后的不同时间点对小鼠进行成像,以评估 CLARITY 生物材料提供类似于靶标的成像对比度的潜力。这项研究还使用尸体来评估 CLARITY 提供人体成像对比度的潜力。结果显示,治疗后长达 30 天的计算机断层扫描 (CT) 和核磁共振成像 (MRI) 显示出成像对比度,证明了作为靶标使用的潜力。与未接受治疗的小鼠相比,接受装载免疫佐剂的CLARITY生物材料治疗的小鼠在治疗后10周内的存活率明显提高(***,p = 0.0006)。这些初步结果表明,CLARITY 生物材料具有作为智能多功能放疗生物材料的潜力,并为进一步开发和优化这种用于放疗和免疫疗法联合治疗的护理点技术提供了动力。
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引用次数: 0
Evaluating the Effects of BSA-Coated Gold Nanorods on Cell Migration Potential and Inflammatory Mediators in Human Dermal Fibroblasts. 评估 BSA 涂层金纳米棒对人真皮成纤维细胞的细胞迁移潜能和炎症介质的影响
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-26 DOI: 10.3390/jfb15100284
Nouf N Mahmoud, Ayat S Hammad, Alaya S Al Kaabi, Hend H Alawi, Summaiya Khatoon, Maha Al-Asmakh

Albumin-coated gold nanoparticles display potential biomedical applications, including cancer research, infection treatment, and wound healing; however, elucidating their interaction with normal cells remains an area with limited exploration. In this study, gold nanorods (GNR) were prepared and coated with bovine serum albumin (BSA) to produce GNR-BSA. The functionalized nanoparticles were characterized based on their optical absorption spectra, morphology, surface charge, and quantity of attached protein. The interaction between GNR-BSA and BSA with normal cells was investigated using human dermal fibroblasts. The cytotoxicity test indicated cell viability between ~63-95% for GNR-BSA over concentrations from 30.0 to 0.47 μg/mL and ~85-98% for BSA over concentrations from 4.0 to 0.0625 mg/mL. The impact of the GNR-BSA and BSA on cell migration potential and wound healing was assessed using scratch assay, and the modulation of cytokine release was explored by quantifying a panel of cytokines using Multiplex technology. The results indicated that GNR-BSA, at 10 μg/mL, delayed the cell migration and wound healing 24 h post-treatment compared to the BSA or the control group with an average wound closure percentage of 6% and 16% at 6 and 24 h post-treatment, respectively. Multiplex analysis revealed that while GNR-BSA reduced the release of the pro-inflammatory marker IL-12 from the activated fibroblasts 24 h post-treatment, they significantly reduced the release of IL-8 (p < 0.001), and CCL2 (p < 0.01), which are crucial for the inflammation response, cell adhesion, proliferation, migration, and angiogenesis. Although GNR-BSA exhibited relatively high cell viability towards human dermal fibroblasts and promising therapeutic applications, toxicity aspects related to cell motility and migration must be considered.

白蛋白涂层金纳米粒子具有潜在的生物医学应用价值,包括癌症研究、感染治疗和伤口愈合;然而,阐明它们与正常细胞的相互作用仍然是一个探索有限的领域。本研究制备了金纳米棒(GNR),并用牛血清白蛋白(BSA)包覆,生成了 GNR-BSA。根据其光学吸收光谱、形态、表面电荷和附着蛋白质的数量对功能化纳米粒子进行了表征。利用人体真皮成纤维细胞研究了 GNR-BSA 和 BSA 与正常细胞的相互作用。细胞毒性测试表明,在 30.0 至 0.47 μg/mL 的浓度范围内,GNR-BSA 的细胞存活率约为 63-95%;在 4.0 至 0.0625 mg/mL 的浓度范围内,BSA 的细胞存活率约为 85-98%。使用划痕试验评估了 GNR-BSA 和 BSA 对细胞迁移潜力和伤口愈合的影响,并使用多重技术量化了一系列细胞因子,从而探讨了细胞因子释放的调节作用。结果表明,与 BSA 或对照组相比,10 μg/mL 的 GNR-BSA 会延缓处理后 24 小时的细胞迁移和伤口愈合,处理后 6 小时和 24 小时的平均伤口闭合率分别为 6% 和 16%。多重分析表明,虽然 GNR-BSA 减少了处理后 24 小时活化成纤维细胞中促炎标志物 IL-12 的释放,但它们显著减少了 IL-8 (p < 0.001)和 CCL2 (p < 0.01)的释放,而这两种标志物对炎症反应、细胞粘附、增殖、迁移和血管生成至关重要。尽管 GNR-BSA 对人真皮成纤维细胞表现出较高的细胞活力,并具有良好的治疗应用前景,但必须考虑与细胞运动和迁移相关的毒性问题。
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Journal of Functional Biomaterials
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