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Investigation of Mechanical and Corrosion Properties of New Mg-Zn-Ga Amorphous Alloys for Biomedical Applications. 用于生物医学应用的新型镁锌镓非晶合金的机械和腐蚀特性研究
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-20 DOI: 10.3390/jfb15090275
Viacheslav E Bazhenov, Mikhail V Gorobinskiy, Andrey I Bazlov, Vasiliy A Bautin, Andrey V Koltygin, Alexander A Komissarov, Denis V Ten, Anna V Li, Alexey Yu Drobyshev, Yoongu Kang, In-Ho Jung, Kwang Seon Shin

Magnesium alloys are considered as promising materials for use as biodegradable implants due to their biocompatibility and similarity to human bone properties. However, their high corrosion rate in bodily fluids limits their use. To address this issue, amorphization can be used to inhibit microgalvanic corrosion and increase corrosion resistance. The Mg-Zn-Ga metallic glass system was investigated in this study, which shows potential for improving the corrosion resistance of magnesium alloys for biodegradable implants. According to clinical tests, it has been demonstrated that Ga ions are effective in the regeneration of bone tissue. The microstructure, phase composition, and phase transition temperatures of sixteen Mg-Zn-Ga alloys were analyzed. In addition, a liquidus projection of the Mg-Zn-Ga system was constructed and validated through the thermodynamic calculations based on the CALPHAD-type database. Furthermore, amorphous ribbons were prepared by rapid solidification of the melt for prospective alloys. XRD and DSC analysis indicate that the alloys with the most potential possess an amorphous structure. The ribbons exhibit an ultimate tensile strength of up to 524 MPa and a low corrosion rate of 0.1-0.3 mm/year in Hanks' solution. Therefore, it appears that Mg-Zn-Ga metallic glass alloys could be suitable for biodegradable applications.

镁合金因其生物相容性和与人体骨骼相似的特性,被认为是有希望用作生物可降解植入体的材料。然而,镁合金在体液中的高腐蚀率限制了其使用。为了解决这个问题,可以利用非晶化来抑制微电化学腐蚀并提高耐腐蚀性。本研究对 Mg-Zn-Ga 金属玻璃系统进行了研究,结果表明该系统具有提高可生物降解植入体镁合金耐腐蚀性的潜力。临床试验证明,镓离子能有效促进骨组织再生。本文分析了 16 种 Mg-Zn-Ga 合金的微观结构、相组成和相变温度。此外,通过基于 CALPHAD 型数据库的热力学计算,构建并验证了 Mg-Zn-Ga 系统的液相投影。此外,还通过熔体的快速凝固制备了未来合金的无定形带。XRD 和 DSC 分析表明,最具潜力的合金具有非晶态结构。这些带材的极限拉伸强度高达 524 兆帕,在汉克斯溶液中的腐蚀速率低至 0.1-0.3 毫米/年。因此,Mg-Zn-Ga 金属玻璃合金似乎适合生物降解应用。
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引用次数: 0
Electrospun Silk-ICG Composite Fibers and the Application toward Hemorrhage Control. 电纺丝-ICG 复合纤维及其在止血方面的应用
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-19 DOI: 10.3390/jfb15090272
Ayesha Siddiqua, Elwin Clutter, Olga Garklavs, Hemalatha Kanniyappan, Rong R Wang

In trauma and surgery, efficient hemorrhage control is crucial to avert fatal blood loss and increase the likelihood of survival. There is a significant demand for novel biomaterials capable of promptly and effectively managing bleeding. This study aimed to develop flexible biocomposite fibrous scaffolds with an electrospinning technique using silk fibroin (SF) and indocyanine green (ICG). The FDA-approved ICG dye has unique photothermal properties. The water permeability, degradability, and biocompatibility of Bombyx mori cocoon-derived SF make it promising for biomedical applications. While as-spun SF-ICG fibers were dissolvable in water, ethanol vapor treatment (EVT) effectively induced secondary structural changes to promote β-sheet formation. This resulted in significantly improved aqueous stability and mechanical strength of the fibers, thereby increasing their fluid uptake capability. The enhanced SF-ICG interaction effectively prevented ICG leaching from the composite fibers, enabling them to generate heat under NIR irradiation due to ICG's photothermal properties. Our results showed that an SF-ICG 0.4% fibrous matrix can uptake 473% water. When water was replaced by bovine blood, a 25 s NIR irradiation induced complete blood coagulation. However, pure silk did not have the same effect. Additionally, NIR irradiation of the SF-ICG fibers successfully stopped the flow of blood in an in vitro model that mimicked a damaged blood vessel. This novel breakthrough offers a biotextile platform poised to enhance patient outcomes across various medical scenarios, representing a significant milestone in functional biomaterials.

在创伤和外科手术中,有效控制出血对于避免致命失血和提高存活率至关重要。对能够及时有效控制出血的新型生物材料的需求量很大。本研究旨在利用丝纤维蛋白(SF)和吲哚菁绿(ICG),通过电纺丝技术开发柔性生物复合纤维支架。美国 FDA 批准的 ICG 染料具有独特的光热特性。从蚕茧中提取的蚕丝纤维素具有透水性、可降解性和生物相容性,因此在生物医学应用中大有可为。虽然原纺丝 SF-ICG 纤维可溶于水,但乙醇蒸汽处理(EVT)可有效诱导二次结构变化,促进 β 片形成。这大大提高了纤维的水稳定性和机械强度,从而增强了其吸液能力。SF-ICG 相互作用的增强有效地防止了 ICG 从复合纤维中浸出,使其能够在近红外照射下利用 ICG 的光热特性产生热量。我们的研究结果表明,SF-ICG 含量为 0.4% 的纤维基质可吸收 473% 的水分。当用牛血代替水时,25 秒的近红外照射可诱导血液完全凝固。然而,纯丝却没有同样的效果。此外,在模拟受损血管的体外模型中,近红外照射 SF-ICG 纤维可成功阻止血液流动。这一新颖的突破提供了一个生物纺织平台,有望在各种医疗场景中提高病人的治疗效果,是功能性生物材料领域的一个重要里程碑。
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引用次数: 0
Application of the Self-Assembling Peptide Hydrogel RADA16 for Hemostasis during Tonsillectomy: A Feasibility Study. 应用自组装多肽水凝胶 RADA16 在扁桃体切除术中止血:可行性研究
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-18 DOI: 10.3390/jfb15090271
Joshua Michaels, Anna I Kaleva, Laura Bateman, Oliver Wakelam, Joanna Stephens

Tonsillectomy is a common surgical procedure but carries a high risk of readmission for secondary bleeding and pain. This study evaluated the feasibility and effectiveness of using the hemostatic self-assembling peptide hydrogel RADA16 (PuraBond, 3-D Matrix SAS; Caluire et Cuire, France) to control bleeding from the tonsillectomy wound bed. Readmission/re-operation rates were compared between a prospective case series of 21 primarily adult tonsillectomy patients treated with topical RADA16 and an untreated historical Control group of 164 patients who underwent tonsillectomy by 10 surgeons at a single tertiary hospital in the UK between March 2019 and June 2022. Cumulative readmission rates for any reason were 2-fold elevated in Control subjects (18.9%; n = 31/164 subjects) compared to patients treated intra-operatively with RADA16 hemostatic hydrogel (9.5%; n = 2/21) (p = 0.378). Readmission rates for postoperative bleeding were 3-fold higher in Controls (14.6%; n = 24/164 subjects) than in the RADA16-treated group (4.8%; n = 1/21) (p = 0.317). A similar rate of retreatment for pain was recorded in the Control (4.3%; n = 7/164) and RADA16 (4.8%; n = 1/21) groups (p = 0.999). Two Control subjects (1.2%) required re-operation for recalcitrant bleeding; no RADA16 subject (0.0%) required re-operation for any reason. No device-related adverse events occurred in the RADA16 group. Surgeons were pleased with the easy learning curve and technical feasibility associated with intra-operatively administering RADA16 hemostatic hydrogel. Intra-operative hemostasis using RADA16 peptide hydrogel was straightforward and was associated with a trend of 3-fold lower rates of readmission for postoperative bleeding events than untreated Control subjects.

扁桃体切除术是一种常见的外科手术,但因继发性出血和疼痛而再次入院的风险很高。本研究评估了使用止血自组装肽水凝胶 RADA16(PuraBond,3-D Matrix SAS;法国 Caluire et Cuire)控制扁桃体切除术伤口床出血的可行性和有效性。前瞻性病例系列包括 21 名接受局部 RADA16 治疗的成人扁桃体切除术患者,未接受治疗的历史对照组包括 164 名患者,这些患者于 2019 年 3 月至 2022 年 6 月期间在英国一家三甲医院接受了由 10 名外科医生主刀的扁桃体切除术。与术中接受 RADA16 止血水凝胶治疗的患者(9.5%;n = 2/21)相比,对照组患者因任何原因的累计再入院率(18.9%;n = 31/164)高出 2 倍(p = 0.378)。对照组术后出血再入院率(14.6%;n = 24/164)是 RADA16 治疗组(4.8%;n = 1/21)的 3 倍(p = 0.317)。对照组(4.3%;n = 7/164)和 RADA16 治疗组(4.8%;n = 1/21)的疼痛再治疗率相似(p = 0.999)。两名对照组受试者(1.2%)因顽固性出血需要再次手术;没有一名 RADA16 受试者(0.0%)因任何原因需要再次手术。RADA16 组未发生与设备相关的不良事件。外科医生对术中使用 RADA16 止血水凝胶的易学性和技术可行性表示满意。使用 RADA16 多肽水凝胶进行术中止血非常简单,而且术后出血事件的再入院率比未经处理的对照组低 3 倍。
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引用次数: 0
Regeneration of Critical Calvarial Bone Defects Using Bovine Xenograft, Magnesium-Enriched Bovine Xenograft and Autologous Dentin in Rats: Micro-CT, Gene Expression and Immunohistochemical Analysis. 使用牛异种移植、高镁牛异种移植和自体牙本质再生大鼠钙骨关键缺损:显微 CT、基因表达和免疫组化分析。
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-18 DOI: 10.3390/jfb15090270
Marija Čandrlić, Ana Terezija Jerbić Radetić, Hrvoje Omrčen, Barbara Franović, Lara Batičić, Tamara Gulić, Tea Čaljkušić-Mance, Sanja Zoričić Cvek, Lucija Malešić, Željka Perić Kačarević, Olga Cvijanović Peloza

The aim of this study was to evaluate the efficacy of autologous dentin (AD), bovine xenograft (BX) and magnesium-enriched bovine xenograft (BX + Mg) in the healing of critical cranial bone defects (CCBDs) in rats. Eighty male Wistar rats were divided into four groups: BX, BX + Mg, AD and the control group (no intervention). Eight mm CCBDs were created and treated with the respective biomaterials. Healing was assessed 7, 15, 21 and 30 days after surgery by micro-computed tomography (micro-CT), real-time polymerase chain reaction (RT-PCR) and immunohistochemical analysis. Micro-CT analysis showed that AD had the highest bone volume and the least amount of residual biomaterial at day 30, indicating robust bone formation and efficient resorption. BX + Mg showed significant bone volume but had more residual biomaterial compared to AD. RT-PCR showed that the expression of osteocalcin (OC), the receptor activator of nuclear factor κB (RANK) and sclerostin (SOST), was highest in the AD group at day 21 and vascular endothelial growth factor (VEGF) at day 15, indicating increased osteogenesis and angiogenesis in the AD group. Immunohistochemical staining confirmed intense BMP-2/4 and SMAD-1/5/8 expression in the AD group, indicating osteoinductive properties. The favorable gene expression profile and biocompatibility of AD and BX + Mg make them promising candidates for clinical applications in bone tissue engineering. Further research is required to fully exploit their potential in regenerative surgery.

本研究旨在评估自体牙本质(AD)、牛异种移植(BX)和富镁牛异种移植(BX + Mg)对大鼠危重颅骨缺损(CCBD)愈合的疗效。80 只雄性 Wistar 大鼠被分为四组:BX组、BX + Mg组、AD组和对照组(无干预)。大鼠的CCBD为8毫米,用相应的生物材料进行治疗。通过微型计算机断层扫描(micro-CT)、实时聚合酶链反应(RT-PCR)和免疫组化分析,对术后 7、15、21 和 30 天的愈合情况进行评估。显微计算机断层扫描分析表明,AD 的骨量最高,而在第 30 天时残留的生物材料最少,这表明骨形成强劲而吸收有效。与AD相比,BX + Mg显示出显著的骨量,但有更多的残留生物材料。RT-PCR显示,骨钙素(OC)、核因子κB受体激活剂(RANK)和硬骨素(SOST)的表达在第21天的AD组最高,血管内皮生长因子(VEGF)的表达在第15天最高,表明AD组的骨形成和血管生成增加。免疫组化染色证实,AD 组中 BMP-2/4 和 SMAD-1/5/8 表达旺盛,表明其具有诱导骨生成的特性。AD 和 BX + Mg 具有良好的基因表达谱和生物相容性,有望在骨组织工程中得到临床应用。要充分挖掘它们在再生手术中的潜力,还需要进一步的研究。
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引用次数: 0
Impact of Calcium Lactate Pretreatment on Enamel Fluoride Uptake: A Comparative In Vitro Study of Different Fluoride Types and Concentrations. 乳酸钙预处理对牙釉质摄氟量的影响:不同氟化物类型和浓度的体外比较研究
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-16 DOI: 10.3390/jfb15090269
Fjolla Kullashi Spahija, Ivana Sutej, Kresimir Basic, Kreshnik Spahija, Kristina Peros

(1) Background: This study aimed to establish the effect of calcium lactate enamel pretreatment related to different fluoride types and concentrations on the enamel uptake of alkali-soluble fluorides. (2) Materials: In a blind and randomized in vitro study, a total of 60 teeth are used. The first 30 teeth were cut and randomly allocated into one of the following treatments: (A) calcium lactate pretreatment followed by three different fluoride solutions; (B) the "Fluoride only" group, with slabs treated with three different fluoride solutions; (C) the "Calcium only" group, with slabs treated with calcium lactate solution; (D) slabs treated with deionized water (negative control group). The next 30 teeth underwent all the above described group procedures but were treated with lower fluoride concentrations. Fluoride was extracted from enamel using 1 M KOH solution and analyzed using a fluoride ion-specific electrode. (3) Results: The findings revealed that slabs treated with NaF following calcium lactate pretreatment exhibited significantly greater enamel uptake of alkali-soluble fluoride compared to other substrates. This significant effect was not observed at lower fluoride concentrations. (4) Conclusion: The study demonstrates that pretreatment with calcium lactate followed by treatment with NaF at 226 ppm F significantly enhances the uptake of alkali-soluble fluoride in enamel compared to other fluoride types.

(1) 背景:本研究旨在确定不同氟化物类型和浓度的乳酸钙釉质预处理对釉质吸收碱溶性氟化物的影响。(2)材料:在一项盲法随机体外研究中,共使用了 60 颗牙齿。切取前 30 颗牙齿,随机分配到以下处理中:(A)乳酸钙预处理,然后用三种不同的氟化物溶液处理;(B)"仅氟化物 "组,用三种不同的氟化物溶液处理牙板;(C)"仅钙 "组,用乳酸钙溶液处理牙板;(D)用去离子水处理牙板(阴性对照组)。接下来的 30 颗牙齿进行了上述所有分组程序,但使用的氟浓度较低。使用 1 M KOH 溶液从牙釉质中提取氟,并使用氟离子特异性电极进行分析。(3) 结果:研究结果表明,与其他基质相比,经乳酸钙预处理后再用 NaF 处理的牙板的釉质对碱溶性氟化物的吸收率明显更高。在氟浓度较低的情况下,没有观察到这种明显的效果。(4) 结论:该研究表明,与其他氟化物类型相比,用乳酸钙预处理后再用百万分之 226 的 NaF 处理,能显著提高釉质对碱溶性氟化物的吸收。
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引用次数: 0
Functional Biomaterials and Biomaterial Composites with Antimicrobial Properties. 具有抗菌特性的功能性生物材料和生物材料复合材料。
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-14 DOI: 10.3390/jfb15090267
John H T Luong

AMR occurs when bacteria, viruses, fungi, and parasites no longer respond to antimicrobial medicines, including antibiotics, antivirals, antifungals, and antiparasitics [...].

当细菌、病毒、真菌和寄生虫不再对抗菌药物(包括抗生素、抗病毒药、抗真菌药和抗寄生虫药)产生反应时,就会出现 AMR [...]。
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引用次数: 0
Stress Distribution of Pediatric Zirconia and Stainless Steel Crowns after Pulpotomy Procedure under Vertical Loading: A Patient-Specific Finite Element Analysis. 垂直载荷下牙髓切断术后小儿氧化锆和不锈钢牙冠的应力分布:针对患者的有限元分析。
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-14 DOI: 10.3390/jfb15090268
Özgür Doğan

Aim: With modern dentistry advancements, children and parents have significantly raised aesthetic expectations in pediatric dentistry. Pediatric zirconia crowns (PZCs) provide a superior aesthetic appearance compared with stainless steel crowns (SSCs), making them a popular treatment option. However, a comparison of the compressive stresses caused by these crowns on the roots of primary teeth and alveolar bones has not been conducted. Materials and Methods: Cone beam computed tomography (CBCT) images of an eight-year-old female patient who experienced premature loss of a primary mandibular left second molar were obtained from a dental hospital database. Rhinoceros 4.0 software was used to process and simulate images. Under simulated chewing forces, stress on the PZC, SSC, and intact primary first molars as control groups, as well as their roots and alveolar bone structures, was assessed with finite element analysis. Statistical Analyses: Depending on whether the descriptive data were normally distributed, the Student t-test and Mann-Whitney U test were used. Quantitative variables differ between the two categories of qualitative variables. One-way ANOVA and Kruskal-Wallis H tests were used depending on standard distribution assumptions. p < 0.05 indicates statistical significance differences. Results: PZCs, SSCs, and cement layers were stressed according to von Mises values, while roots and alveolar bones were stressed according to maximum and minimum stress values. When assessing crowns, SSCs exhibited the highest von Mises stress values, followed by PZCs and control groups (p < 0.001). In the cement layer, SSCs obtained significantly higher values (p = 0.003). In the root area, minimum principal stress values are more critical. The highest values were obtained from the intact tooth, PZC, and SSC, respectively (p < 0.001). Alveolar bones did not differ significantly in minimum principal stress (p = 0.950). Conclusions: Restorative full-coverage crowns exhibited higher von Mises values than intact teeth, as per current research findings. The von Mises values were highest in SSC, while lowest in PZC. As a result of this condition, the cement layer and root areas had higher von Mises stress and compressive stress. Alveolar bones were not affected regardless of restoration type. PZC transmits higher stress due to its properties.

目的:随着现代牙科技术的进步,儿童和家长对儿童牙科的审美要求也大大提高。与不锈钢冠(SSC)相比,儿童氧化锆冠(PZC)具有更优越的美学外观,因此成为一种流行的治疗选择。然而,关于这些牙冠对基牙牙根和牙槽骨造成的压应力的比较还没有进行过。材料和方法:从一家牙科医院的数据库中获取了一名八岁女性患者的锥形束计算机断层扫描(CBCT)图像,该患者的下颌左侧第二磨牙过早脱落。使用 Rhinoceros 4.0 软件处理和模拟图像。在模拟咀嚼力的作用下,通过有限元分析评估了作为对照组的 PZC、SSC 和完整的初级第一磨牙及其牙根和牙槽骨结构的应力。统计分析:根据描述性数据是否呈正态分布,采用学生 t 检验和 Mann-Whitney U 检验。定量变量在两类定性变量之间存在差异。根据标准分布假设,采用单因子方差分析和 Kruskal-Wallis H 检验。结果PZC、SSC和骨水泥层的应力是根据von Mises值计算的,而牙根和牙槽骨的应力是根据最大和最小应力值计算的。在评估牙冠时,SSCs 的 von Mises 应力值最高,其次是 PZCs 和对照组(p < 0.001)。在水泥层中,SSCs 的应力值明显更高(p = 0.003)。在根部区域,最小主应力值更为关键。完整牙齿、PZC 和 SSC 分别获得了最高值(p < 0.001)。牙槽骨在最小主应力方面没有明显差异(p = 0.950)。结论根据目前的研究结果,全覆盖修复冠的 von Mises 值高于完整牙齿。SSC 的 von Mises 值最高,而 PZC 的最低。在这种情况下,骨水泥层和牙根区域具有更高的 von Mises 应力和压缩应力。无论哪种修复类型,牙槽骨都没有受到影响。PZC 因其特性可传递更高的应力。
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引用次数: 0
In Vitro Wound-Healing Potential of Phenolic and Polysaccharide Extracts of Aloe vera Gel. 芦荟凝胶酚类和多糖提取物的体外伤口愈合潜力。
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-13 DOI: 10.3390/jfb15090266
Andreea Iosageanu, Elena Mihai, Ana-Maria Seciu-Grama, Elena Utoiu, Alexandra Gaspar-Pintiliescu, Florentina Gatea, Anisoara Cimpean, Oana Craciunescu

The present study aimed to conduct a comparative investigation of the biological properties of phenolic and polysaccharide extracts obtained using an ultrasound-assisted technique from Aloe vera gel and their effects on each stage of the wound healing process in in vitro experimental models. HPLC analysis showed that the phenolic extract contained aloin, ferulic, and caffeic acid, as well as quercetin dihydrate, as major compounds. Capillary zone electrophoresis indicated the prevalence of mannose and glucose in the polysaccharide extract. Cell culture testing revealed the anti-inflammatory properties of the phenolic extract at a concentration of 0.25 mg/mL through significant inhibition of pro-inflammatory cytokines-up to 28% TNF-α and 11% IL-8 secretion-in inflamed THP-1-derived macrophages, while a pro-inflammatory effect was observed at 0.5 mg/mL. The phenolic extract induced 18% stimulation of L929 fibroblast proliferation at a concentration of 0.5 mg/mL, enhanced the cell migration rate by 20%, and increased collagen type I synthesis by 18%. Moreover, the phenolic extract exhibited superior antioxidant properties by scavenging free DPPH (IC50 of 2.50 mg/mL) and ABTS (16.47 mM TE/g) radicals, and 46% inhibition of intracellular reactive oxygen species (ROS) production was achieved. The polysaccharide extract demonstrated a greater increase in collagen synthesis up to 25%, as well as antibacterial activity against Staphylococcus aureus with a bacteriostatic effect at 25 mg/mL and a bactericidal one at 50 mg/mL. All these findings indicate that the phenolic extract might be more beneficial in formulations intended for the initial phases of wound healing, such as inflammation and proliferation, while the polysaccharide extract could be more suitable for use during the remodeling stage. Moreover, they might be combined with other biomaterials, acting as efficient dressings with anti-inflammatory, antioxidant, and antibacterial properties for rapid recovery of chronic wounds.

本研究旨在对利用超声辅助技术从芦荟凝胶中提取的酚类和多糖类提取物的生物特性及其对体外实验模型中伤口愈合过程各阶段的影响进行比较研究。高效液相色谱分析显示,酚类提取物中含有芦荟素、阿魏酸、咖啡酸以及二水槲皮素等主要化合物。毛细管区带电泳显示,多糖提取物中普遍含有甘露糖和葡萄糖。细胞培养测试表明,浓度为 0.25 mg/mL 的酚类提取物具有抗炎特性,能显著抑制促炎细胞因子--28% 的 TNF-α 和 11% 的 IL-8 在发炎的 THP-1 衍生巨噬细胞中的分泌,而浓度为 0.5 mg/mL 时则有促炎作用。在 0.5 mg/mL 浓度下,酚提取物对 L929 成纤维细胞增殖的刺激率为 18%,细胞迁移率提高了 20%,I 型胶原蛋白的合成增加了 18%。此外,酚类提取物通过清除游离 DPPH(IC50 为 2.50 mg/mL)和 ABTS(16.47 mM TE/g)自由基表现出卓越的抗氧化性,对细胞内活性氧(ROS)产生的抑制率达到 46%。多糖提取物显示胶原蛋白合成增加了 25%,并对金黄色葡萄球菌具有抗菌活性,25 毫克/毫升时具有抑菌作用,50 毫克/毫升时具有杀菌作用。所有这些发现都表明,酚类提取物可能更适合用于伤口愈合初期的配方,如炎症和增殖阶段,而多糖提取物可能更适合用于重塑阶段。此外,它们还可以与其他生物材料相结合,作为具有消炎、抗氧化和抗菌特性的高效敷料,用于慢性伤口的快速恢复。
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引用次数: 0
Comparison of Key Properties of Ag-TiO2 and Hydroxyapatite-Ag-TiO2 Coatings on NiTi SMA. 镍钛 SMA 上的 Ag-TiO2 涂层和羟基磷灰石-Ag-TiO2 涂层的关键性能比较。
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-12 DOI: 10.3390/jfb15090264
Karolina Dudek, Aleksandra Strach, Daniel Wasilkowski, Bożena Łosiewicz, Julian Kubisztal, Anna Mrozek-Wilczkiewicz, Patryk Zioła, Adrian Barylski

To functionalize the NiTi alloy, multifunctional innovative nanocoatings of Ag-TiO2 and Ag-TiO2 doped with hydroxyapatite were engineered on its surface. The coatings were thoroughly characterized, focusing on surface topography and key functional properties, including adhesion, surface wettability, biocompatibility, antibacterial activity, and corrosion resistance. The electrochemical corrosion kinetics in a simulated body fluid and the mechanisms were analyzed. The coatings exhibited hydrophilic properties and were biocompatible with fibroblast and osteoblast cells while also demonstrating antibacterial activity against E. coli and S. epidermidis. The coatings adhered strongly to the NiTi substrate, with superior adhesion observed in the hydroxyapatite-doped layers. Conversely, the Ag-TiO2 layers showed enhanced corrosion resistance.

为了使镍钛合金功能化,在其表面设计了 Ag-TiO2 和掺杂羟基磷灰石的 Ag-TiO2 多功能创新纳米涂层。对涂层进行了全面的表征,重点是表面形貌和关键功能特性,包括附着力、表面润湿性、生物相容性、抗菌活性和耐腐蚀性。还分析了模拟体液中的电化学腐蚀动力学及其机理。涂层具有亲水性,与成纤维细胞和成骨细胞具有生物相容性,同时对大肠杆菌和表皮葡萄球菌具有抗菌活性。涂层与镍钛基底的附着力很强,掺羟基磷灰石的涂层附着力更强。相反,Ag-TiO2 涂层则显示出更强的耐腐蚀性。
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引用次数: 0
Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation. 探索纤维蛋白凝胶在促进细胞迁移以形成血管方面的作用
IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-12 DOI: 10.3390/jfb15090265
Joana A Moura, Hugh J Barlow, Shareen H Doak, Karl Hawkins, Iris Muller, Martin J D Clift

A hallmark of angiogenesis is the sprouting of endothelial cells. To replicate this event in vitro, biomaterial approaches can play an essential role in promoting cell migration. To study the capacity of a scaffold of fibrin (fibrinogen:thrombin mix) to support the movement of the endothelial cells, the migration area of spheroids formed with the HULEC cell line was measured. The cells were first allowed to form a spheroid using the hanging drop technique before being encapsulated in the fibrin gel. The cells' migration area was then measured after two days of embedding in the fibrin gel. Various conditions affecting fibrin gel polymerization, such as different concentrations of fibrinogen and thrombin, were evaluated alongside rheology, porosity, and fiber thickness analysis to understand how these factors influenced cell behavior within the composite biomaterial. Data point toward thrombin's role in governing fibrin gel polymerization; higher concentrations result in less rigid gels (loss tangent between 0.07 and 0.034) and increased cell migration (maximum concentration tested: 5 U/mL). The herein presented method allows for a more precise determination of the crosslinking conditions of fibrin gel that can be used to stimulate angiogenic sprouting.

血管生成的一个标志是内皮细胞的萌发。要在体外复制这一过程,生物材料方法可在促进细胞迁移方面发挥重要作用。为了研究纤维蛋白支架(纤维蛋白原:凝血酶混合物)支持内皮细胞迁移的能力,我们测量了用 HULEC 细胞系形成的球形细胞的迁移面积。在将细胞包裹在纤维蛋白凝胶中之前,首先使用悬滴技术让细胞形成球形。然后在纤维蛋白凝胶中包埋两天后测量细胞的迁移面积。除了流变学、孔隙率和纤维厚度分析外,还对影响纤维蛋白凝胶聚合的各种条件(如不同浓度的纤维蛋白原和凝血酶)进行了评估,以了解这些因素如何影响细胞在复合生物材料中的行为。数据表明凝血酶在调节纤维蛋白凝胶聚合中的作用;浓度越高,凝胶硬度越低(损失正切在 0.07 和 0.034 之间),细胞迁移率越高(测试的最大浓度:5 U/mL)。本文介绍的方法可以更精确地确定纤维蛋白凝胶的交联条件,从而用于刺激血管新生萌芽。
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Journal of Functional Biomaterials
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