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IF 1.6 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2025-08-15 DOI: 10.1016/S0737-0806(25)00325-9
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引用次数: 0
Butylated hydroxytoluene (BHT) improved semen quality and sperm DNA of frozen-thawed Arabian stallions preserved in modified INRA-82 extender 丁基羟基甲苯(BHT)改善了在改良INRA-82扩展器中保存的冻融阿拉伯种马的精液质量和精子DNA。
IF 1.6 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2025-08-14 DOI: 10.1016/j.jevs.2025.105675
Amal M. Aboelmaaty , Islam El-Sayed El-Seadawy , Mohamed Saeed Kotp , Alaa A. Mohamed , Hazem Ahmed El-Debaky , Diya A. El-Badry , Abeer M. Anwer , Tsvetan Tsvetkov , Teodora Daneva , Mahmoud A. Elgabry

Background

Alpha-tocopherol is one of the non-enzymatic lipophilic antioxidants. Butylated hydroxytoluene (BHT) is a synthetic analog with similar modes of action in protecting the cryopreserved sperms.

Aims/objectives

This study hypothesized that a certain concentration of any antioxidant is suitable for improving the post-thaw semen quality of stallions.

Methods

To determine the optimum BHT concentration, a synthetic antioxidant similar to vitamin E in potency and scavenging oxidative stress power in concentrations of 0.0, 0.25, 0.50, 1.0, 2.0, and 4.0 mM/ml were added to semen extender. The post-thaw sperm progressive motility at 0, 1 h, 2 h, and 3 h, the sperm viability index, the plasma membrane integrity tested by the hypo-osmotic swelling test (HOST), the acrosome integrity, non-fragmented DNA, % of DNA in the comet head, % of DNA in the comet tail, comet tail length, and comet tail moment were compared.

Results

According to our hypothesis, 1.0 mM BHT was the most suitable concentration that preserved the highest (P < 0.0001) post-thaw sperm progressive motility at 0.0, 1 h, 2 h, 3 h., the highest viability index (P < 0.0001), plasma membrane and acrosome integrities (P < 0.0001),non-fragmented DNA (P < 0.001), and % of DNA in the comet head (P < 0.001), the lowest DNA % in the comet tail (P < 0.001) and comet tail moment (P < 0.0001), and the shortest (P < 0.0001) comet tail length.

Conclusions

The concentrations of BHT around 1.0 mM/ml (0.5, 2.0mM/ml) proved better post-thaw semen characteristics, but increasing BHT concentrations from >2.0mM/ml to 4.0 mM/ml started to deteriorate semen quality than non-supplemented control. The optimum BHT concentration for modified INRA-82 is 1.0 mM/ml.
背景:α -生育酚是一种非酶促亲脂抗氧化剂。丁基羟基甲苯(BHT)是一种合成类似物,在保护冷冻保存精子方面具有类似的作用模式。目的:本研究假设一定浓度的抗氧化剂可改善种马解冻后精液质量。方法:在精液膨化剂中分别添加浓度为0.0、0.25、0.50、1.0、2.0和4.0 mM/ml的合成抗氧化剂,以确定BHT的最佳浓度。比较解冻后精子0、1h、2h、3h的进展活力、精子活力指数、低渗透膨胀试验(HOST)检测的质膜完整性、顶体完整性、非片段化DNA、彗头DNA百分比、彗尾DNA百分比、彗尾长度和彗尾瞬间。结论:1.0mM /ml(0.5、2.0mM/ml)前后BHT浓度较好,但当BHT浓度从0 ~ 2.0mM/ml增加到4.0 mM/ml时,精液质量较未添加BHT的对照组开始恶化。改性INRA-82的最佳BHT浓度为1.0 mM/ml。
{"title":"Butylated hydroxytoluene (BHT) improved semen quality and sperm DNA of frozen-thawed Arabian stallions preserved in modified INRA-82 extender","authors":"Amal M. Aboelmaaty ,&nbsp;Islam El-Sayed El-Seadawy ,&nbsp;Mohamed Saeed Kotp ,&nbsp;Alaa A. Mohamed ,&nbsp;Hazem Ahmed El-Debaky ,&nbsp;Diya A. El-Badry ,&nbsp;Abeer M. Anwer ,&nbsp;Tsvetan Tsvetkov ,&nbsp;Teodora Daneva ,&nbsp;Mahmoud A. Elgabry","doi":"10.1016/j.jevs.2025.105675","DOIUrl":"10.1016/j.jevs.2025.105675","url":null,"abstract":"<div><h3>Background</h3><div>Alpha-tocopherol is one of the non-enzymatic lipophilic antioxidants. Butylated hydroxytoluene (BHT) is a synthetic analog with similar modes of action in protecting the cryopreserved sperms.</div></div><div><h3>Aims/objectives</h3><div>This study hypothesized that a certain concentration of any antioxidant is suitable for improving the post-thaw semen quality of stallions.</div></div><div><h3>Methods</h3><div>To determine the optimum BHT concentration, a synthetic antioxidant similar to vitamin E in potency and scavenging oxidative stress power in concentrations of 0.0, 0.25, 0.50, 1.0, 2.0, and 4.0 mM/ml were added to semen extender. The post-thaw sperm progressive motility at 0, 1 h, 2 h, and 3 h, the sperm viability index, the plasma membrane integrity tested by the hypo-osmotic swelling test (HOST), the acrosome integrity, non-fragmented DNA, % of DNA in the comet head, % of DNA in the comet tail, comet tail length, and comet tail moment were compared.</div></div><div><h3>Results</h3><div>According to our hypothesis, 1.0 mM BHT was the most suitable concentration that preserved the highest (<em>P</em> &lt; 0.0001) post-thaw sperm progressive motility at 0.0, 1 h, 2 h, 3 h., the highest viability index (<em>P</em> &lt; 0.0001), plasma membrane and acrosome integrities (<em>P</em> &lt; 0.0001),non-fragmented DNA (<em>P</em> &lt; 0.001), and % of DNA in the comet head (<em>P</em> &lt; 0.001), the lowest DNA % in the comet tail (<em>P</em> &lt; 0.001) and comet tail moment (<em>P</em> &lt; 0.0001), and the shortest (<em>P</em> &lt; 0.0001) comet tail length.</div></div><div><h3>Conclusions</h3><div>The concentrations of BHT around 1.0 mM/ml (0.5, 2.0mM/ml) proved better post-thaw semen characteristics, but increasing BHT concentrations from &gt;2.0mM/ml to 4.0 mM/ml started to deteriorate semen quality than non-supplemented control. The optimum BHT concentration for modified INRA-82 is 1.0 mM/ml.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"153 ","pages":"Article 105675"},"PeriodicalIF":1.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144862249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Photodynamic therapy in the treatment of cutaneous habronemosis in a horse: Case report 光动力疗法治疗马皮肤线虫病一例报告。
IF 1.6 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2025-08-13 DOI: 10.1016/j.jevs.2025.105674
I. Ferreira , J.G. Pinto , E.G.L. Oliveira , M.C.B.S. de Azevedo , L.M.C. Marcolino , J. Ferreira-Strixino
Habronema muscae is a parasitic nematode that causes habronemiasis in horses, leading to chronic skin lesions. The parasite is transmitted via flies, and larvae deposited in wounds result in difficult-to-heal “summer sores”. This case study involved treating a 9-year-old gelding male horse with recurrent habronemiasis using photodynamic therapy (PDT) concurrent with conventional treatments. The horse presented with ulcerated, exudative wounds on its limbs and face, unresponsive to traditional treatments. PDT was applied over six months, using methylene blue (MB) as a photosensitizer, combined with LED irradiation. Additionally, an ivermectin-based ointment and other healing agents were used. The results showed a reduction in lesion size after two months, complete healing of the thoracic wounds by month four, and facial wound healing after six months. In this case, PDT was an effective adjunct treatment for managing habronemiasis.
肌肉Habronema muscae是一种寄生虫,可引起马的Habronema病,导致慢性皮肤损伤。这种寄生虫通过苍蝇传播,幼虫沉积在伤口上导致难以愈合的“夏季疮”。本病例研究涉及使用光动力疗法(PDT)结合常规治疗治疗一匹复发性habronemasis的9岁公马。这匹马的四肢和面部出现溃烂、渗出的伤口,对传统治疗无效。PDT应用超过6个月,使用亚甲基蓝(MB)作为光敏剂,结合LED照射。此外,还使用了伊维菌素软膏和其他愈合剂。结果显示,2个月后病变大小减小,4个月后胸部伤口完全愈合,6个月后面部伤口愈合。在这种情况下,PDT是一种有效的辅助治疗管理血红素病。
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引用次数: 0
Emergence of a new alveolar dental element after extraction of a molar tooth in a horse 拔除马的一颗臼齿后出现新的牙槽。
IF 1.6 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2025-08-11 DOI: 10.1016/j.jevs.2025.105673
L.L.V. Oliveira , M.C.A.R. Caldas , L.M.E. Marval , C.A. Marval , J.G.S. Carvalho , J.J.R.T. Matos , B.D. Malacarne , C.F.R. Paz
A 2-year-old mixed-breed mare was examined at the property due to swelling and fistula on the right mandible. Oral examination and radiographs revealed the presence of an apical abscess involving the 410 tooth. The tooth was extracted entirely and confirmed radiographically. One year later, the owner reported a reoccurrence of clinical signs. A new radiographic evaluation revealed a radiopaque area in the alveolar socket of the 410 tooth filled with spongy-looking bone. An extraoral approach to the alveolar socket was performed, and the content removed had the appearance of a dental element.
一匹2岁的混血母马因右下颌骨肿胀和瘘管而被检查。口腔检查及x光片显示410颗牙齿有根尖脓肿。整颗牙齿被拔了出来并做了放射检查。一年后,业主报告临床症状再次出现。新的x线片评估显示410牙的牙槽窝有一个不透射线的区域,里面充满了海绵状的骨头。通过口外入路到达牙槽窝,取出的内容物具有牙体的外观。
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引用次数: 0
Management of retained fetal membranes by umbilical vessel infusion in mares submitted to elective C-section 母马择期剖宫产时脐带血管灌注处理胎膜残留。
IF 1.6 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2025-08-06 DOI: 10.1016/j.jevs.2025.105662
Marcos Eduardo Neto , Bruna R. Curcio , Leandro A. Rafael , Giovana M. Pivato , Gabriela C. Silva , Rafaela P. Souza , Mariana A. Mousquer , Carlos E.W. Nogueira
The cesarian section (C-section) is a potential risk factor for retained fetal membranes (RFM) in mares, requiring prompt diagnosis and treatment to enhance outcomes. This study aimed to evaluate the use of umbilical vessel infusion for the treatment of RFM after elective C-section in mares. Ten pregnant criollo type-mares at 315 days of gestation were monitored daily and underwent elective C-section upon readiness for birth. All mares exhibited RFM, with placentas retained beyond three hours careful extraction of the foal by C-section. Those were randomly assigned into two groups: Umbilical vessel infusion (n = 5): using of low-pressure 0.9 % saline solution infusion into the umbilical vessel; or Counterweight (n = 5): involving a light counterweight (1 kg) attached to the exposed fetal membrane. Mares that presented time from onset treatment to full separation and expulsion of the fetal membrane by 40 min were classified as responsive to treatment. There were no differences in mares’ age (8 ± 2, 3-20 years), gestational length (333 ± 3, 328-360 d), foal weight (38 ± 2, 30-50 Kg), the surgical time to C-section (124 ± 5, 90-150 min), and time of uterine manipulation during C-section (57 ± 3, 45-70 min) between groups. Results showed that 80 % (n = 4/5) of mares in the Umbilical Vessel Infusion group achieved membrane expulsion within 40 minutes, while none (0 %, n = 0/5) in the Counterweight group responded within this timeframe (P < 0.05). Mares receiving the umbilical vessel infusion method have not experienced severe signs of discomfort, pain, or additional secondary side effects after the procedure. In conclusion, umbilical vessel infusion is an effective, safe, and practical method for the treatment of retained fetal membranes after C-section in mares.
剖宫产(c -剖宫产)是母马保留胎膜(RFM)的潜在危险因素,需要及时诊断和治疗以提高预后。本研究旨在评估脐带血管输注治疗母马择期剖腹产后RFM的应用。每天监测10匹怀孕315天的克里奥罗型母马,并在准备分娩时进行选择性剖腹产。所有的母马都表现出RFM,胎盘保留超过三小时,小心地通过剖腹产提取马驹。随机分为两组:脐管输注组(n=5):脐管输注0.9%低压生理盐水;或配重(n=5):包括附着在暴露的胎膜上的轻配重(1kg)。从开始治疗到胎膜完全分离和排出40分钟的母马被归类为对治疗有反应。母马年龄(8±2,3 ~ 20岁)、妊娠期(333±3,328 ~ 360 d)、马驹体重(38±2,30 ~ 50 Kg)、剖宫产手术时间(124±5,90 ~ 150 min)、剖宫产术中操作子宫时间(57±3,45 ~ 70 min)组间差异无统计学意义。结果显示,脐管输注组80% (n=4/5)的母马在40分钟内出现排膜现象,而配重组没有(0%,n=0/5)的母马在40分钟内出现排膜现象(P < 0.05)。接受脐带血管输注方法的母马在手术后没有出现严重的不适、疼痛或其他次要副作用的迹象。综上所述,脐带血管灌注是一种有效、安全、实用的治疗母马剖宫产后胎膜残留的方法。
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引用次数: 0
Advanced equine duodenoscopy technique 先进的马十二指肠镜技术
IF 1.6 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2025-07-30 DOI: 10.1016/j.jevs.2025.105659
R. Agrícola , G. Ribeiro , J. Borges , L. Carvalho , I. Roquet , L.M. Carvalho , J. Prazeres
Duodenal pathologies are underreported in equine medicine, probably not due to little occurrence, but due to the limitations of current diagnostic techniques. The aim of this report is to describe a novel duodenoscopic approach, that allows a complete visualization of a region inaccessible via standard techniques. Gastroduodenoscopy was performed in a clinical setting in 60 adult horses referred to us due to complaints suspected to be related to gastroduodenal diseases. The initial steps of the procedure were performed using previously published techniques. Once the video-endoscope reached the cranial duodenum and before direct visualization of the major duodenal papilla, a slow and controlled retroflexion maneuver was performed, allowing a complete examination of the duodenal face of the pyloric sphincter and the whole cranial aspect of the duodenum. The technique described allowed better duodenoscopy examination, without risking lesions of the adjacent mucosa and allowed a wide field of view avoiding blind spots.
十二指肠病变在马医学中被低估,可能不是因为很少发生,而是由于当前诊断技术的限制。本报告的目的是描述一种新的十二指肠镜方法,允许通过标准技术无法实现的区域的完整可视化。胃十二指肠镜检查是在临床设置在60成年马转介给我们,由于投诉怀疑与胃十二指肠疾病有关。该过程的最初步骤是使用先前发表的技术进行的。一旦视频内窥镜到达颅十二指肠,在直接看到主要十二指肠乳头之前,进行缓慢和控制的后旋操作,允许对幽门括约肌的十二指肠面和十二指肠的整个颅面进行完整检查。所描述的技术可以更好地进行十二指肠镜检查,而不会危及邻近粘膜的病变,并允许宽视野避免盲点。
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引用次数: 0
Plasma Activin A concentrations are not a useful biomarker for detecting insulin dysregulation and predicting laminitis risk in ponies 血浆激活素A浓度不是检测胰岛素失调和预测小马板炎风险的有用生物标志物。
IF 1.6 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2025-07-29 DOI: 10.1016/j.jevs.2025.105660
C.J. McGuire , E.J. Knowles , P.A. Harris , N.J. Menzies-Gow

Background

Circulating insulin concentrations are used to quantify risk of laminitis in currently non-laminitic ponies. Basal serum activin A (AA) concentration has previously been positively correlated with serum insulin concentrations 60 minutes (T60) following an oral sugar test (OST) in ponies with equine metabolic syndrome. Therefore, circulating AA might be a useful marker for insulin dysregulation (ID) and laminitis risk.

Objectives

To explore the relationship between circulating AA concentrations at baseline (T0) and T60 in ponies that developed laminitis within six months and non-laminitic ponies.

Methods

Case-control study. Forty-three ponies that developed laminitis (PLP) during a four-year surveillance period were selected from a larger cohort; 43 ponies from the same cohort that remained non-laminitic (NLP) were selected as controls. Plasma AA concentrations were measured using a validated ELISA at T0 and T60 and compared between groups at both time points using a Wilcoxon signed ranks test. Correlations with other previously measured metabolic markers were investigated using Spearman‘s rank correlation coefficients.

Results

There was a significant difference between T0 plasma AA (median 8.8; interquartile range [6.2, 13.1]ng/mL) and T60 (13.1; [8.8, 20.1]ng/mL) within the NLP group (P= <0.001) and between plasma AA at T0 (7.4 [5.5, 12.4]ng/mL) and T60 (12.3 [6.9, 16.6]ng/ml) in the PLP group (P= <0.001). There were no significant correlations between plasma AA (T0 or T60) and any other measured metabolic marker. Single timepoints and retrospective analysis were the main limitations.

Conclusion

Plasma AA concentration is not a useful marker for ID or predicting laminitis development in ponies.
背景:循环胰岛素浓度用于量化目前非板层炎小马板层炎的风险。在患有马代谢综合征的小马中,基础血清激活素A (AA)浓度与口服糖试验(OST)后60分钟(T60)的血清胰岛素浓度呈正相关。因此,循环AA可能是胰岛素失调(ID)和板炎风险的有用标记。目的:探讨6个月内发生板层炎的小马和非板层炎小马的基线循环AA浓度(T0)和T60之间的关系。方法:病例-对照研究。在四年的监测期间,从一个更大的队列中选择了43匹患板部炎(PLP)的小马;从同一队列中选择43匹非带状疱疹(NLP)小马作为对照。在T0和T60时使用有效的ELISA测定血浆AA浓度,并在两个时间点使用Wilcoxon符号秩检验进行组间比较。与其他先前测量的代谢标志物的相关性使用秩相关系数进行研究。结果:T0血浆AA(中位8.8;四分位间距[6.2,13.1]ng/mL)和T60 (13.1;[8.8, 20.1]ng/mL) (P=)结论:血浆AA浓度不能作为马体ID或预测蹄板炎发展的有效指标。
{"title":"Plasma Activin A concentrations are not a useful biomarker for detecting insulin dysregulation and predicting laminitis risk in ponies","authors":"C.J. McGuire ,&nbsp;E.J. Knowles ,&nbsp;P.A. Harris ,&nbsp;N.J. Menzies-Gow","doi":"10.1016/j.jevs.2025.105660","DOIUrl":"10.1016/j.jevs.2025.105660","url":null,"abstract":"<div><h3>Background</h3><div>Circulating insulin concentrations are used to quantify risk of laminitis in currently non-laminitic ponies. Basal serum activin A (AA) concentration has previously been positively correlated with serum insulin concentrations 60 minutes (T60) following an oral sugar test (OST) in ponies with equine metabolic syndrome. Therefore, circulating AA might be a useful marker for insulin dysregulation (ID) and laminitis risk.</div></div><div><h3>Objectives</h3><div>To explore the relationship between circulating AA concentrations at baseline (T0) and T60 in ponies that developed laminitis within six months and non-laminitic ponies.</div></div><div><h3>Methods</h3><div>Case-control study. Forty-three ponies that developed laminitis (PLP) during a four-year surveillance period were selected from a larger cohort; 43 ponies from the same cohort that remained non-laminitic (NLP) were selected as controls. Plasma AA concentrations were measured using a validated ELISA at T0 and T60 and compared between groups at both time points using a Wilcoxon signed ranks test. Correlations with other previously measured metabolic markers were investigated using Spearman‘s rank correlation coefficients.</div></div><div><h3>Results</h3><div>There was a significant difference between T0 plasma AA (median 8.8; interquartile range [6.2, 13.1]ng/mL) and T60 (13.1; [8.8, 20.1]ng/mL) within the NLP group (P= &lt;0.001) and between plasma AA at T0 (7.4 [5.5, 12.4]ng/mL) and T60 (12.3 [6.9, 16.6]ng/ml) in the PLP group (P= &lt;0.001). There were no significant correlations between plasma AA (T0 or T60) and any other measured metabolic marker. Single timepoints and retrospective analysis were the main limitations.</div></div><div><h3>Conclusion</h3><div>Plasma AA concentration is not a useful marker for ID or predicting laminitis development in ponies.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"153 ","pages":"Article 105660"},"PeriodicalIF":1.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Population structure and signature of selection for body size in some Iranian and exotic horse breeds 一些伊朗和外来马品种的种群结构和体型选择特征。
IF 1.6 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2025-07-29 DOI: 10.1016/j.jevs.2025.105658
Banafsheh Bazvand , Amir Rashidi , Mohammad Bagher Zandi , Mohammad Hossein Moradi , Jalal Rostamzadeh , Peyman Mahmoudi

Background

Iran is home to genetically distinct horse breeds shaped by geography and history. Breeds like the Kurdish, Caspian pony, and Turkmen reflect deep-rooted diversity and unique adaptive traits.

Aims/Objectives

This study aimed to identify genomic regions exhibiting signatures of selection and to detect candidate genes and biological pathways related to body size that may influence performance in various Iranian and exotic horse breeds.

Methods

Samples were collected from three Iranian native breeds (Caspian pony, Turkmen, and Kurdish) and three exotic breeds (Fell pony, Arabian, and Akhal-Teke) originating from England, the Middle East, and Turkmenistan, respectively. Genotyping data included 50 K SNPs for the exotic breeds and 70 K SNPs for the Iranian breeds. To explore population structure and genetic differentiation, Discriminant Analysis of Principal Components (DAPC) was conducted.

Results

Population structure analysis showed clear separation between pony and light horse breeds, with Akhal-Teke and Turkmen clustering together and Fell ponies forming a distinct group. Using FLK and hapFLK tests, we identified 124 and 127 candidate genes under selection, respectively. Several genes were linked to body size traits, including those involved in bone mineralization, osteoblast differentiation, growth factor signaling, and tissue development. Notably, we identified novel genes including SAMD7, SPARC1, IBSP, MEPE, DEUP12, PLAAT1, and SPP1 that not previously reported in horse studies, offering new insights into equine morphology.

Conclusion

Results revealed selection signatures linked to genes influencing body size and development, including several novel candidates. These findings deepen our understanding of equine morphology and warrant further investigation using larger sample sizes.
背景:伊朗是由地理和历史形成的遗传上独特的马品种的家园。像库尔德马、里海马和土库曼马这样的品种反映了根深蒂固的多样性和独特的适应特征。目的/目的:本研究旨在确定表现出选择特征的基因组区域,并检测可能影响各种伊朗和外来马品种表现的与体型相关的候选基因和生物学途径。方法:分别采集来自英国、中东和土库曼斯坦的3个伊朗本土品种(里海马、土库曼马和库尔德马)和3个外来品种(费尔马、阿拉伯马和阿克哈尔-特克马)。基因分型数据包括外来品种的50K个snp和伊朗品种的70K个snp。为探究种群结构和遗传分化,采用主成分判别分析(DAPC)方法。结果:种群结构分析显示,矮马和轻马品种之间存在明显的分离,阿克哈-特克马和土库曼马聚集在一起,费尔马形成一个独立的群体。通过FLK和hapFLK测试,我们分别鉴定出124个和127个候选基因。有几个基因与体型特征有关,包括那些与骨矿化、成骨细胞分化、生长因子信号传导和组织发育有关的基因。值得注意的是,我们发现了新的基因,包括SAMD7、SPARC1、IBSP、MEPE、DEUP12、PLAAT1和SPP1,这些基因以前没有在马的研究中报道过,为马的形态学提供了新的见解。结论:结果揭示了与影响体型和发育的基因相关的选择特征,包括一些新的候选基因。这些发现加深了我们对马形态的理解,并保证了进一步的研究使用更大的样本量。
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引用次数: 0
Clinical safety of intrauterine infusion of a uterine sanitizer in mares 母马宫内输注子宫消毒液的临床安全性。
IF 1.6 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2025-07-29 DOI: 10.1016/j.jevs.2025.105657
Lorenzo Segabinazzi , Fernando P. Lisboa , Bruna Zutter

Background

Limited response to conventional therapies and rising antibiotic resistance emphasize the need for alternative treatments for infectious endometritis. A recent in vitro study showed promising antimicrobial activity of a commercial uterine cleaning sanitizer (UCS) against endometritis pathogens. However, its safety and efficacy in mares remain untested.

Aims/objectives

This study aimed to assess the clinical safety of intrauterine infusion of UCS in reproductively sound mares.

Methods

Exp. 1. Six healthy mares with endometrial biopsies classified as I or IIA had two intrauterine infusions of UCS performed during estrus. Biopsies were taken 3 days post-treatment and 8- and 21-days post-ovulation. Exp. 2. Eleven reproductive sound mares had two estrous cycles randomly assigned to receive two intrauterine infusions of UCS or placebo (lactate ringer) at the beginning of estrus. Mares were inseminated 24 h after the last treatment. Embryo flushing was performed 8 days post-ovulation.

Results

Exp. 1. There was a slight change in endometrial biopsy scores, primarily due to an increased inflammatory infiltrate in the endometrium, three days post-treatment with complete recovery to baselines at 8- and 21-days post-ovulation. Exp. 2. Embryo recovery rates were similar between UCS (9/11) and placebo (8/11) cycles.

Conclusion

In conclusion, intrauterine infusion of UCS appears to be a safe therapy in broodmare practice and may offer a promising adjuvant therapy for endometritis in mares.
背景:对常规治疗的有限反应和不断上升的抗生素耐药性强调了感染性子宫内膜炎替代治疗的必要性。最近的一项体外研究表明,一种商业子宫清洁消毒剂(UCS)对子宫内膜炎病原体具有良好的抗菌活性。然而,它在母马身上的安全性和有效性尚未得到检验。目的:本研究旨在评估生殖健全母马宫内输注UCS的临床安全性。方法:实验1。6匹子宫内膜活检分类为I或IIA的健康母马在发情期间两次宫内输注UCS。治疗后3天、排卵后8天和21天分别行活检。Exp。2。11匹生殖健全的母马有两个发情周期,在发情开始时随机分配两次宫内输注UCS或安慰剂(乳酸林格)。末次给药后24h进行人工授精。排卵后8天进行胚胎冲洗。结果:实验1。子宫内膜活检评分略有变化,主要是由于子宫内膜炎症浸润增加,治疗后3天,排卵后8天和21天完全恢复到基线。Exp。2。胚胎恢复率在UCS(9/11)和安慰剂(8/11)周期之间相似。结论:综上所述,子宫内灌注UCS是一种安全的治疗方法,可能是治疗母马子宫内膜炎的一种有前景的辅助治疗方法。
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引用次数: 0
How accurate are riders in assessing saddle position? 骑手对马鞍位置的判断有多准确?
IF 1.3 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2025-07-21 DOI: 10.1016/j.jevs.2025.105655
M. Douglas , J. Williams , H. Randle

Background

Recent research has highlighted the detrimental impact of an ill-fitting saddle on equine health, welfare and performance. A correctly fitting saddle may not fit if positioned incorrectly.

Aims

This study assessed riders’ ability to visually evaluate saddle position and examine factors that influenced accuracy of assessment.

Methods

Survey participants were provided with photographs of a series of saddles on horses obtained from the lateral aspect of the left side of the horse. Saddles were either correctly positioned (n = 5), too far forward (n = 5) or too far back (n = 2). This was independently confirmed by three accredited saddle fitting professionals. The survey was distributed via snowball sampling on social media over an 3-week period in 2021.

Results

There were 401 participants, who, on average, correctly identified 58.25 % of the saddle positions. Riders’ ability to correctly assess saddle position (mean 58.25 % correct) was not related to rider age, experience and discipline (all Kruskal-Wallis tests P > 0.05). However, participants who had previously had their own saddle fitted professionally were better able to correctly assess saddle position (60.8 ± 19.0 % correct) compared to those who had not (52.2 ± 19.0 %; KW (1) = 12.59, P < 0.001).

Conclusion

Given a substantial proportion of riders in this study were unable to identify correct saddle position these results are concerning due to a likely increased risk of causing the horse discomfort and compromising welfare. Given the increasing focus on ethical and sustainable equitation practice, this lack of knowledge regarding a fundamental piece of horse-riding equipment must be addressed as a priority.
背景:最近的研究强调了一个不合适的马鞍对马的健康,福利和性能的不利影响。如果位置不正确,一个正确的鞍座可能不合适。目的:本研究评估骑手对鞍位的视觉评估能力,并探讨影响评估准确性的因素。方法:向调查参与者提供了从马左侧侧面获得的一系列马鞍的照片。马鞍位置正确(n=5),太靠前(n=5)或太靠后(n=2)。这是由三名认可的马鞍安装专业人员独立确认的。该调查于2021年在社交媒体上以滚雪球抽样的方式进行,为期三周。结果:共有401名参与者,平均正确识别出58.25%的马鞍位置。骑手正确评估马鞍位置的能力(平均58.25%的正确率)与骑手的年龄、经验和纪律无关(所有Kruskal-Wallis检验P < 0.05)。然而,先前专业安装过马鞍的参与者能够更好地正确评估马鞍位置(60.8±19.0%正确率),而没有安装马鞍的参与者(52.2±19.0%;Kw(1) = 12.59,p < 0.001)。结论:考虑到本研究中相当大比例的骑手无法识别正确的马鞍位置,这些结果令人担忧,因为这可能会增加导致马匹不适和损害福利的风险。鉴于对道德和可持续马术实践的日益关注,必须优先解决关于骑马基本装备知识缺乏的问题。
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引用次数: 0
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Journal of Equine Veterinary Science
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