Journal of Equine Veterinary Science最新文献_第8页

The tipping point: Exploring the influence of rider presence and posture on gait parameters in horses working in mounted equine-assisted services 临界点：探索骑手在场和姿势对马在骑马辅助服务中工作的步态参数的影响。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-09-10 DOI: 10.1016/j.jevs.2025.105699

B.H. Baxley , A. Stellato , N.C. Anderson

Background

Horses involved in equine-assisted services (EAS) support the psychological and physiological health of individuals through mounted activities; however, limited research exists on how these sessions affect the horse’s movement.

Aim

This study aimed to investigate the influence of rider presence and posture on horses’ gait parameters in EAS settings.

Methods

Horses (n = 22) were observed during walk and trot, both with and without a mounted rider, in four EAS centers in the United States. Distal limb-mounted inertial measurement units collected peak ground reaction force (pGRF), stride duration, stride symmetry, stride length, and stationary proportion. Rider posture angular metrics were calculated using static images. Mixed linear regression models assessed effects of rider presence, posture, life stage, and horse-related factors (e.g., age, breed) on each gait parameter.

Results

Rider presence increased pGRF (walk: +2.36 N, P < 0.01; trot: +6.69 N, P < 0.001) increased and stride length (walk: +0.13 m, P < 0.001; trot: +0.23 m, P < 0.001). Stationary proportion increased during the walk (+2.2 %, P = 0.03). Rider left trunk lean increased forelimb pGRF (walk: +0.39, P = 0.03) and stationary proportion (trot: +0.68, P = 0.02). Draft cross breeds (vs. cobs) had increased pGRF (walk: +14.04, P < 0.01; trot: +27.74, P = 0.02), and stride length (trot: +0.82, P = 0.02). Child (vs. adult) riders increased stationary proportion (trot: +4.47, P = 0.04) and reduced stride duration (trot:52.41, P < 0.01).

Conclusion

Rider presence, posture, and rider life stage influenced horse gait in EAS contexts. These findings highlight the importance of considering rider-horse interactions in EAS when assessing horse welfare and longevity.

背景：参与马辅助服务（EAS）的马通过骑马活动支持个体的心理和生理健康；然而，关于这些训练如何影响马的运动的研究有限。目的：探讨骑手在场和姿态对EAS环境下马的步态参数的影响。方法：在美国的四个EAS中心，观察了马（n=22）在步行和小跑时的情况，包括有和没有骑马的骑手。安装在远端肢体上的惯性测量单元收集了峰值地面反作用力（pGRF）、步幅持续时间、步幅对称性、步幅长度和静止比例。使用静态图像计算骑手姿态角度量。混合线性回归模型评估骑手存在、姿势、生命阶段和马相关因素（如年龄、品种）对每个步态参数的影响。结果：骑手在场增加了马的pGRF（步行：+2.36 N, p）。结论：骑手在场、骑手姿势和骑手生命阶段影响EAS环境下马的步态。这些发现强调了在评估马的福利和寿命时，考虑EAS中骑手与马的相互作用的重要性。

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引用次数: 0

Preliminary insights on the daily rhythm of CRP and IL-6 in athletic horses 对运动马CRP和IL-6日常节律的初步认识。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-09-10 DOI: 10.1016/j.jevs.2025.105702

F. Aragona , M. Rizzo , F. Arfuso , F. Arrigo , F. Fazio , E. Giudice , C. Faggio , G. Piccione , C. Giannetto

Background

Homeostasis and inflammation are two opposing physiological processes that are driven by the circadian clock.

Aims/objectives

The study aim was to investigate the biological rhythm of the C-reactive protein (CRP) and Interleukin-6 (IL-6) in horses.

Methods

Eight clinically healthy Italian saddle gelding horses, housed in individual boxes and subjected to a natural 12/12 L/D cycle, were enrolled. Blood samples were performed every 4 hours over a 48-hour period.

Results

A positive correlation was found between CRP and IL-6 on both days of monitoring. Both parameters showed a daily rhythm; CRP acrophase was in the middle of the scotophase, IL-6 acrophase at the beginning of the light phase, and both parameters had a high percentage of robustness (>75%).

Conclusion

This preliminary information improves the knowledge about the daily rhythm and possible correlation of some inflammatory biomarkers in horses. Further studies are necessary to investigate how different environmental conditions, management, and physical exercise might influence this rhythmicity.

背景：体内平衡和炎症是由生物钟驱动的两个相反的生理过程。目的：研究马体内c反应蛋白（CRP）和白细胞介素-6 （IL-6）的生物节律。方法：选取8匹临床健康的意大利马鞍骟马，分别饲养于单独的马箱内，进行自然的12/12 L/D循环。在48小时内每4小时采集一次血样。结果：CRP与IL-6在监测的2天均呈正相关。这两个参数都显示出每日节律；CRP顶相在暗相中期，IL-6顶相在光相开始，两个参数的稳健性百分比都很高（>75%）。结论：这一初步信息提高了对马的日常节律和一些炎症生物标志物可能的相关性的认识。需要进一步研究不同的环境条件、管理和体育锻炼如何影响这种节律性。

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引用次数: 0

Intrinsic characteristics of donkeys that affect drug treatment 影响药物治疗的驴的内在特征。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-09-10 DOI: 10.1016/j.jevs.2025.105700

Ignacio Lizarraga , Fernanda Castillo-Alcala

Donkeys have contributed to the development and livelihoods of humans for almost 7,000 years. They have been used for traction and transportation, kept as pets, utilized in animal-assisted therapies, or farmed as food-producing animals. Over the past two decades, significant progress has been made in bridging the knowledge gap in veterinary medicine between donkeys and horses. Veterinary practitioners are now more aware that donkeys have important behavioral, anatomical, and physiological characteristics that distinguish them from horses. These peculiarities should be considered when treating donkeys. For instance, donkeys are highly efficient in compartmentalizing and conserving water and metabolizing xenobiotics via cytochrome P450 enzymes. This affects the selection of drugs and their dosages. Estimating live weight and recognising and assessing pain accurately are also important factors when instituting pharmacological treatments for donkeys. Therapeutic regimens have been established for some drugs in donkeys, but many are still directly extrapolated from horses. Because donkeys are classified as a minor species, there are relatively few medications approved for their use. In addition, some cultures also consider donkeys as to have lower economic value than horses, which may preclude donkeys from receiving appropriate veterinary care. Veterinary education on donkey-specific topics would improve the health and ultimately the welfare of these animals. This review explores the characteristics of donkeys and offers guidance on their care and treatment, aiming to enhance their overall quality of life.

近7000年来，驴子为人类的发展和生计做出了贡献。它们被用来牵引和运输，作为宠物饲养，用于动物辅助治疗，或作为生产食物的动物养殖。在过去二十年中，在弥合驴和马在兽医学方面的知识差距方面取得了重大进展。兽医从业人员现在更加意识到驴子具有重要的行为、解剖和生理特征，这些特征使它们区别于马。在治疗驴的时候应该考虑到这些特点。例如，驴通过细胞色素P450酶在划分和保存水分和代谢异种生物方面效率很高。这影响了药物的选择和剂量。在对驴进行药物治疗时，估计活重和准确识别和评估疼痛也是重要因素。一些药物已经在驴身上建立了治疗方案，但许多药物仍然是直接从马身上推断出来的。由于驴被归类为次要物种，因此批准用于它们的药物相对较少。此外，一些文化还认为驴的经济价值低于马，这可能会使驴得不到适当的兽医照顾。以驴为主题的兽医教育将改善这些动物的健康，并最终改善它们的福利。这篇综述探讨了驴子的特点，并为它们的护理和治疗提供了指导，旨在提高它们的整体生活质量。

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引用次数: 0

Effects of sand, asphalt, and a three degrees hind toe or heel elevation on equine cervical and thoracolumbosacral kinematics at walk and trot 沙子、沥青和脚趾或脚跟后抬高3度对马在步行和小跑时颈椎和胸腰骶骨运动学的影响。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-09-04 DOI: 10.1016/j.jevs.2025.105697

S. Caure , K. Miljak , Ph. Dendaw , L. Thesée , E. Villedey , A. Malinvaud , M. Cousty , V. Prié , K. Horan , R. Weller

Background

Effects of ground surface and hoof angles on equine cervical and thoracolumbosacral kinematics are poorly understood. However, the equine cervical and thoracolumbosacral areas present frequent lesions and he management of factors that might improve treatment and rehabilitation outcomes, such as ground surface and hoof angles, requires more investigation.

Aims

Our objectives were to determine the influence of ground surface (asphalt versus sand) and a 3 degrees hind toe or heel elevation on cervical and thoracolumbosacral kinematics during walking and trotting.

Methods

Six saddle horses were equipped with reflective markers and shod with reference shoes (REF), consisting of fore aluminium (REF F) and hind steel racehorse (REF H) shoes. Two-dimensional videos, performed with a camera placed perpendicularly to the horse, were used to compare kinematic parameters when walking and trotting in hand on asphalt and sand. On asphalt, REF was also compared with REF F and a modified REF H using additional three degrees hind toe or heel wedges.

Results

During walking or trotting, horses showed increased mid-cervical flexion (2±0.8°), cervicothoracic extension (5±1.5°) and thoracolumbar flexion (2.3±0.8°), and a reduction in lumbosacral range of motion (3±0.8°) on asphalt versus sand. Hind heel or toe elevation increased both adaptative cervicothoracic flexion (5±1.6°) and lumbosacral extension (3±0.9°) at walk, relative to REF. An increased plantar hoof angle induced more lumbosacral extension (3±1.3°) at walk. At trot, toe elevation reduced thoracothoracic flexion (2.7±1.1°) and heel elevation reduced thoracothoracic extension (2.9±1.1°).

Conclusion

These observations could be useful for clinical, rehabilitation, training, and performance assessments.

背景：地面表面和蹄角对马颈椎和胸腰骶骨运动学的影响尚不清楚。然而，马的颈椎和胸腰骶骨区域经常出现病变，如何处理可能改善治疗和康复结果的因素，如地面和蹄角，需要更多的研究。目的：我们的目的是确定地面表面（沥青与沙子）和3度后趾或脚跟抬高对行走和小跑期间颈椎和胸腰骶运动学的影响。方法：在6匹马鞍上安装反射标志，并穿参考蹄（REF），参考蹄由前铝蹄（REF）和后钢蹄（REF）组成。用垂直放置在马身上的摄像机拍摄二维视频，用来比较马在沥青和沙子上走路和小跑时的运动学参数。在沥青路面上，REF还与REF F和改良REF H进行了比较，REF F和REF H使用了额外的三度后趾或后跟楔。结果：在步行或小跑时，马在沥青路面上比在沙地上表现出颈中屈（2±0.8°）、颈胸伸（5±1.5°）和胸腰屈（2.3±0.8°）的增加，腰骶活动范围（3±0.8°）的减少。与REF相比，后脚跟或脚趾抬高可增加步行时的适应性颈胸屈曲（5±1.6°）和腰骶伸展（3±0.9°）。足底蹄角增加可增加步行时的腰骶伸展（3±1.3°）。小跑时，脚趾抬高可降低胸廓屈曲度（2.7±1.1°），脚跟抬高可降低胸廓伸度（2.9±1.1°）。结论：这些观察结果可用于临床、康复、训练和绩效评估。

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引用次数: 0

Readers Information 读者信息

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-09-04 DOI: 10.1016/S0737-0806(25)00349-1

引用次数: 0

Time-activity budget in horses and ponies: A systematic review and meta-analysis on feeding dynamics and management implications 马和矮种马的时间-活动预算：对喂养动力学和管理意义的系统回顾和荟萃分析。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-09-03 DOI: 10.1016/j.jevs.2025.105684

M. Lamanna , G. Buonaiuto , R. Colleluori , F. Raspa , E. Valle , D. Cavallini

Background

The time-activity budget is a key indicator of animal welfare. This meta-analysis integrated 14 studies (1979-2020) with 364 horses under wild, natural-living, and stabled conditions to evaluate feeding, resting, standing, and locomotion.

Aim

The study aimed to categorize daily behavioural patterns in equines, with emphasis on feeding behaviour.

Methods

Fixed and random effects models, forest plots, and ANOVA assessed the influence of management, sociality, sex, age, body size, and feeding type. Results: Feeding was greater in free-ranging (56 %) than stabled horses (38 %; p < 0.0001), in grouped (54 %) vs. isolated (39 %; p = 0.0052), in females (64 %) vs. males (48 %; p = 0.0253), and in grazing (56 %) vs. hay-fed horses (39 %; p = 0.0003). Resting was higher in young, small, grouped, and grazing horses. Standing was commoner in stabled, isolated, adult, larger, and hay-fed animals. Locomotion increased in free-ranging, grouped, and grazing horses.

Conclusion

Management promoting foraging, social interaction, and locomotion enhances equine welfare.

时间-活动预算是动物福利的一个关键指标。这项荟萃分析整合了14项研究（1979-2020）的数据，涉及364匹马在不同的管理条件下（野生、自然生活和马厩），以评估喂养、休息、站立和运动行为。采用固定效应和随机效应模型、森林样地和方差分析来评估管理制度、社会性、性别、年龄、体型和饲养类型的影响。饲养时间在管理（散养：56%，马厩：38%，p < 0.0001）、社会住房（群居：54%，隔离：39%，p = 0.0052）、性别（雌性：64%，雄性：48%，混合：46%，p = 0.0253）和饲养方式（放牧：56%，干草喂养：39%，p = 0.0003）方面存在显著差异。休息时间受年龄（3岁：6%；p < 0.0001）、体型（小马：24%，马：9%；p = 0.0005）、群居（群居：20%，孤立：6%；p = 0.0120）和饲喂类型（牧草：20%，干草：9%；p = 0.0418）的影响。站在稳定的更常见(47%)比自由放养的马(25%;p = 0.0013),在孤立的(51%)和分组(25%;p = 0.0002),成人(44%)和年轻的马(24%;p = 0.0047),在马(41%)和小马(23%;p = 0.0088),和hay-fed(47%)和放牧马(24%;p = 0.0004)。自由放养马的运动能力（7.5%）高于圈养马（4.5%;p = 0.0001），分组马（6.6%）高于隔离马（4.5%;p = 0.0220），放牧马（7.5%）高于干草喂养马（4.5%;p = 0.0001）。这项研究强调了促进觅食、社会互动和运动的管理实践的重要性，从而支持马的福利。

{"title":"Time-activity budget in horses and ponies: A systematic review and meta-analysis on feeding dynamics and management implications","authors":"M. Lamanna , G. Buonaiuto , R. Colleluori , F. Raspa , E. Valle , D. Cavallini","doi":"10.1016/j.jevs.2025.105684","DOIUrl":"10.1016/j.jevs.2025.105684","url":null,"abstract":"<div><h3>Background</h3><div>The time-activity budget is a key indicator of animal welfare. This meta-analysis integrated 14 studies (1979-2020) with 364 horses under wild, natural-living, and stabled conditions to evaluate feeding, resting, standing, and locomotion.</div></div><div><h3>Aim</h3><div>The study aimed to categorize daily behavioural patterns in equines, with emphasis on feeding behaviour.</div></div><div><h3>Methods</h3><div>Fixed and random effects models, forest plots, and ANOVA assessed the influence of management, sociality, sex, age, body size, and feeding type. <em>Results:</em> Feeding was greater in free-ranging (56 %) than stabled horses (38 %; <em>p</em> < 0.0001), in grouped (54 %) vs. isolated (39 %; <em>p</em> = 0.0052), in females (64 %) vs. males (48 %; <em>p</em> = 0.0253), and in grazing (56 %) vs. hay-fed horses (39 %; <em>p</em> = 0.0003). Resting was higher in young, small, grouped, and grazing horses. Standing was commoner in stabled, isolated, adult, larger, and hay-fed animals. Locomotion increased in free-ranging, grouped, and grazing horses.</div></div><div><h3>Conclusion</h3><div>Management promoting foraging, social interaction, and locomotion enhances equine welfare.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105684"},"PeriodicalIF":1.6,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ionic composition of gastric reflux and small intestinal fluid in horses: implications for fluid replacement 马胃反流和小肠液的离子组成：对液体置换的影响

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-09-02 DOI: 10.1016/j.jevs.2025.105683

G.C. Paganelli, H.C. Schott II

Background

Small intestinal (SI) distension and gastric reflux (GR) are common in horses with mechanical and functional ileus. Removal of GR results in fluid/ion losses.

Aims/objectives

1) Determine the capacity of healthy SI; 2) measure ion concentrations in normal SI fluid, GR, or fluid from SI undergoing resection. The authors hypothesized that Na⁺ and Cl^- concentrations would be lower and higher, respectively, in GR as compared to SI fluid from healthy horses, while there would be little difference in K⁺ concentration.

Methods

Observational study: two to 15 meters of SI from 15 horses were distended with water to 6-7 cm in diameter. Volume drained was measured to determine capacity. Ion concentrations were measured in SI fluid from healthy horses (n=10), GR (n=11), and strangulated bowel (n=2).

Results

Normal SI had a fluid capacity of 2.8 ± 0.6 L/m. Na⁺, K⁺, and Cl^- concentrations were 100 ± 6, 22 ± 5, and 66 ± 30 mmol/L, respectively, in normal SI fluid; 77 ± 16, 16 ± 11, and 96 ± 25 mmol/L, respectively, in GR; and 116 ± 11, 12 ± 5, and 43 ± 27 mmol/L, respectively, in fluid from strangulated SI. Concentrations of Na⁺ were lower (P<0.01) and Cl^- greater (P<0.03) in GR than in normal SI fluid or fluid from strangulated SI, while all fluid K⁺ concentrations were 3-5-fold greater than serum K⁺ concentration.

Conclusion

These data can be used to estimate water and ion losses that occur with SI disorders to implement appropriate fluid therapy plans.

背景：小肠（SI）膨胀和胃反流（GR）在机械和功能性肠梗阻的马中很常见。去除GR会导致流体/离子损失。目的/目标：1)确定健康SI的能力；2)测量正常SI液、GR或切除SI液中的离子浓度。作者假设，与健康马的SI液相比，GR中的Na+和Cl-浓度分别较低和较高，而K+浓度差异不大。方法：观察研究：取15匹马2 ~ 15米的SI，加水膨胀至直径6 ~ 7cm。测量排干的体积以确定容量。在健康马（n=10）、GR马（n=11）和绞窄肠（n=2）的SI液中测量离子浓度。结果：正常SI的液体容量为2.8±0.6 L/m。正常SI液中Na+、K+和Cl-浓度分别为100±6、22±5和66±30 mmol/L；GR分别为77±16、16±11、96±25 mmol/L；和116±11、12±5和43±27 mmol/L的绞窄性SI液。Na+浓度较低（P-高），P+浓度为血清K+浓度的3-5倍。结论：这些数据可用于估计SI疾病发生的水和离子损失，以实施适当的液体治疗计划。

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引用次数: 0

Stable microclimates and autonomic modulation in horses housed in different stable architectures during monsoon in a tropical environment 稳定的小气候和热带环境中不同稳定建筑中马的自主调节。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-09-01 DOI: 10.1016/j.jevs.2025.105685

Chanoknun Poochipakorn , Kanokpan Sanigavatee , Kansuda Leelehapongsathon , Thita Wonghanchao , Metha Chanda

Background

The impact of climate conditions and stable design on horses housed in individual stalls plays a significant role in their well-being, especially in tropical climates. Limited information exists regarding their conditions during the monsoon season.

Objective

This study focused on the stable microclimate and autonomic regulation of horses kept in different stable architectures during the monsoon in a tropical setting.

Methods

Twenty-two horses were assigned to one of three stable designs, each offering unique microclimates, including relative humidity, air temperature, and various levels of noxious gases. Autonomic modulation was assessed by observing multiple heart rate variability (HRV) metrics at one-hour intervals for 24 consecutive hours.

Results

The findings revealed significant variations in stable microclimates across the different designs, with notable differences between day and night. Horses housed in stables with solid external walls and a lower volume-to-horse ratio exhibited lower HRV (p < 0.05-0.0001), indicating more significant stress, compared to those in stables with solid walls and a higher volume-to-horse ratio or those without solid external walls. Furthermore, the study established a correlation between stable microclimates and HRV modulation based on stable design.

Conclusion

These results indicate that autonomic responses vary among horses in different stable designs, suggesting that stable architecture, microclimates, and the volume-to-horse ratio significantly influence horses’ stress responses. Overall, these findings offer important insights for improving housing management to enhance the welfare of horses during the monsoon season in tropical environments.

背景：气候条件和马厩设计对马的影响在它们的健康中起着重要的作用，特别是在热带气候下。有关它们在季风季节的情况的资料有限。目的：研究热带季风季节不同马厩马的稳定小气候及自主调节。方法：22匹马被分配到三个马厩设计中的一个，每个马厩提供独特的小气候，包括相对湿度，空气温度和不同程度的有害气体。通过连续24小时每隔1小时观察多个心率变异性（HRV）指标来评估自主调节。结果：研究结果显示，在不同的设计中，稳定的微气候存在显著差异，白天和黑夜之间存在显著差异。外墙坚固、体马比较低的马厩的马HRV较低（p < 0.05 ~ 0.0001），表明与外墙坚固、体马比较高或无外墙坚固的马厩相比，压力更大。在稳定设计的基础上，建立了稳定小气候与HRV调制的相关性。结论：不同马厩设计条件下马的自主神经反应存在差异，表明马厩结构、小气候和马体比对马的应激反应有显著影响。总的来说，这些发现为改善住房管理提供了重要的见解，以提高热带季风季节马的福利。

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引用次数: 0

In vitro and in vivo effects of Acacia mearnsii De Wild extract for cyathostomin control in horses 野生金合欢提取物对马胞口蛋白的体内外抑制作用。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-09-01 DOI: 10.1016/j.jevs.2025.105686

G.L.S. Silva , U.A. Curcio , J.A. Boufleur , D.G.L. Borges , M.T.M. Nakatani , M.G. Freitas , F.A. Borges , F.J.C. Faria , R.H.P. Silva , G.L. Franco

Introduction

Anthelmintic resistance has led to the use of organic extracts as alternative methods of parasite control.

Objectives

The study aimed to assess the effects of Acacia mearnsii extract (tannin) on the control of cyathostomins in horses, both in vitro and in vivo.

Materials and methods

Thirty Pantaneiro horses naturally infected with cyathostomins were sourced from two distinct farms, designated as Farms A and B. At the start of the study, all third-stage larvae were cyathostomins, and an egg hatchability test (EHT) was performed using fecal samples from horses on both farms. Two randomized 28-day field studies were conducted for the fecal egg count reduction test (FECRT) with evaluations on days 0, 7, 14, 21, and 28. On days 0 and 28, body weight (BW), body condition score (BCS), and blood samples for hematological and biochemical analysis were also collected. The horses diet included panicum grass pasture, 1 kg of concentrate, 40 g of liquid sugarcane molasses (Control), and 25 g of A. mearnsii extract.

Results

The EC50 values were 13.2 mg/mL (95 % confidence interval [CI]: 11.8–14.7 mg/mL) and 14.9 mg/mL (95 % CI: 13.3–16.9 mg/mL) for Farms A and B, respectively. The FECRT showed no effect (P > 0.05) between the control and treated groups, with mean values of 3.09% and 3.18%, respectively. Blood and hematological parameters also showed no differences (P > 0.05) between treatments.

Conclusion

The tannin extract reduced egg hatchability in vitro, but did not lower fecal egg counts in naturally infected horses during the in vivo trial.

导言：驱虫虫的耐药性已经导致使用有机提取物作为寄生虫控制的替代方法。目的：研究金合欢提取物（单宁）在体外和体内对马胞口蛋白的控制作用。材料和方法：30匹天然感染cyathostomins的Pantaneiro马来自两个不同的农场，分别被指定为A农场和b农场。在研究开始时，所有的第三期幼虫都是cyathostomins，并使用两个农场马的粪便样本进行蛋孵化试验（EHT）。对粪卵计数减少试验（FECRT）进行了两项为期28天的随机现场研究，分别在第0、7、14、21和28天进行评估。第0天和第28天采集体重（BW）、体况评分（BCS），并采集血液进行血液学和生化分析。马饲粮为：马尾草、浓缩物1 kg、甘蔗液体糖蜜（对照）40 g、马尾草提取物25 g。结果：A场EC50值为13.2 mg/mL(95%可信区间[CI]: 11.8 ~ 14.7 mg/mL)， B场EC50值为14.9 mg/mL（95%可信区间：13.3 ~ 16.9 mg/mL）。对照组与治疗组FECRT差异无统计学意义（P < 0.05），平均值分别为3.09和3.18。两组间血液及血液学指标差异无统计学意义（P < 0.05）。结论：单宁提取物在体外降低了卵的孵化率，但在体内试验中并未降低自然感染马的粪便卵数。

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引用次数: 0

Clinical evaluation of the ADVIA Centaur XPT chemiluminescent immunoassay for equine insulin measurement. ADVIA Centaur XPT化学发光免疫分析法测定马胰岛素的临床评价。

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science

Pub Date : 2025-09-01 Epub Date: 2025-07-31 DOI: 10.1016/j.jevs.2025.105661

R Rey-Conejo, R E Toribio, S Möller, E Müller, P Fores-Jackson

Background: Insulin dysregulation is a key component of equine metabolic syndrome and is commonly assessed using basal insulin concentrations. Different analyzers may yield variable insulin results, limiting comparability.

Aims/objectives: To assess the analytical performance of the ADVIA Centaur XPT chemiluminescent immunoassay (CLIA) for equine insulin measurement and to establish adapted decision thresholds based on comparison with a previously used CLIA method.

Methods: Precision, linearity, and dilution recovery were assessed for the ADVIA Centaur XPT. A total of 89 equine serum samples submitted for basal insulin testing were measured using the ADVIA Centaur XPT and Immulite 2000 XPi analyzers. Results were compared using Deming regression, Bland-Altman analysis, and weighted kappa statistics to evaluate agreement in classification.

Results: Intra-assay coefficients of variation were 3.56 %, 2.01 %, and 1.92 % for low, medium, and high insulin concentrations, respectively; inter-assay variation was 5.19 %, 5.78 %, and 5.68 %. Deming regression showed a proportional bias, with the ADVIA Centaur XPT consistently measuring lower insulin concentrations compared to the Immulite 2000 XPi. Bland-Altman analysis confirmed this bias across the measurement range. Spearman correlation between the two methods was 0.92 (95 % CI: 0.87-0.94; P < 0.0001), indicating a strong association in rank order. Classification agreement using adapted decision limits yielded a weighted kappa of 0.82, indicating strong agreement.

Conclusion: The ADVIA Centaur XPT demonstrated acceptable precision. When insulin concentrations were classified using adapted thresholds, the agreement between the two CLIA analyzers was strong, suggesting that the ADVIA Centaur XPT may be suitable for measurement of equine insulin concentration.

背景：胰岛素失调是马代谢综合征的一个关键组成部分，通常用基础胰岛素浓度来评估。不同的分析仪可能产生不同的胰岛素结果，限制了可比性。目的/目的：评估ADVIA Centaur XPT化学发光免疫分析法（CLIA）用于马胰岛素测量的分析性能，并根据与先前使用的CLIA方法的比较建立适应的决策阈值。方法：对ADVIA Centaur XPT的精密度、线性度和稀释回收率进行评价。使用ADVIA Centaur XPT和Immulite 2000 XPi分析仪对提交的89份马血清样本进行基础胰岛素测试。比较结果采用Deming回归、Bland-Altman分析和加权kappa统计来评估分类的一致性。结果：低、中、高胰岛素浓度的测定内变异系数分别为3.56%、2.01%、1.92%；测定间变异率分别为5.19%、5.78%和5.68%。Deming回归显示出比例偏差，与Immulite 2000 XPi相比，ADVIA Centaur XPT持续测量较低的胰岛素浓度。Bland-Altman分析在测量范围内证实了这种偏差。两种方法的Spearman相关性为0.92 (95% CI: 0.87-0.94；P < 0.0001)，表明在等级顺序上有很强的相关性。使用适应决策限制的分类一致产生0.82的加权kappa，表明高度一致。结论：ADVIA Centaur XPT具有可接受的精度。当使用适应阈值对胰岛素浓度进行分类时，两种CLIA分析仪之间的一致性很强，这表明ADVIA Centaur XPT可能适用于测量马胰岛素浓度。

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